S R Sprang

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc A method to determine 18 O kinetic isotope effects in the hydrolysis of nucleotide triphosphates
    Xinlin Du
    Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Anal Biochem 372:213-21. 2008
  2. ncbi request reprint G proteins, effectors and GAPs: structure and mechanism
    S R Sprang
    Howard Hughes Medical Institute, University of Texas, Southwestern Medical Center, Dallas 75235 9050, USA
    Curr Opin Struct Biol 7:849-56. 1997
  3. ncbi request reprint G protein mechanisms: insights from structural analysis
    S R Sprang
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Annu Rev Biochem 66:639-78. 1997
  4. ncbi request reprint Structure of the GDP-Pi complex of Gly203-->Ala gialpha1: a mimic of the ternary product complex of galpha-catalyzed GTP hydrolysis
    A M Berghuis
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75235 9050, USA
    Structure 4:1277-90. 1996
  5. ncbi request reprint Two-metal-Ion catalysis in adenylyl cyclase
    J J Tesmer
    Howard Hughes Medical Institute, Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9050, USA
    Science 285:756-60. 1999
  6. ncbi request reprint Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis
    J J Tesmer
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 89:251-61. 1997
  7. ncbi request reprint Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS
    J J Tesmer
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235 9050, USA
    Science 278:1907-16. 1997
  8. ncbi request reprint The A326S mutant of Gialpha1 as an approximation of the receptor-bound state
    B A Posner
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 273:21752-8. 1998
  9. ncbi request reprint Crystal structure of the adenylyl cyclase activator Gsalpha
    R K Sunahara
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9041, USA
    Science 278:1943-7. 1997
  10. ncbi request reprint Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis
    D E Coleman
    Howard Hughes Medical Institute, Dallas, TX
    Science 265:1405-12. 1994

Collaborators

  • J J Tesmer
  • E Lee
  • S C Sinha
  • R A Johnson
  • Z Chen
  • B Beutler
  • Jürgen U Linder
  • Roland Seifert
  • Xinlin Du
  • Anirban Adhikari
  • Andreas Gille
  • A G Gilman
  • Tung Chung Mou
  • Tara L Davis
  • William W Ja
  • D E Coleman
  • Celestine J Thomas
  • Tsan Xiao
  • B A Posner
  • T Xiao
  • Kurt Ferguson
  • Robert Gregory
  • M A Wall
  • A M Berghuis
  • R B Sutton
  • C W Dessauer
  • Richard W Roberts
  • Alan V Smrcka
  • Tabetha M Bonacci
  • David A Fancy
  • Ryan J Austin
  • PiLong Li
  • R K Sunahara
  • Ying Wang
  • Fred P Abramson
  • Paolo Lecchi
  • Gavin E Black
  • Elliott M Ross
  • Hui Yu Liu
  • Kevin H Gardner
  • P Towb
  • S A Wasserman
  • M B Mixon
  • M J Eck
  • A S Raw
  • J A Iñiguez-Lluhí
  • M E Linder
  • E Rinderknecht
  • J D Zhang
  • M Ultsch
  • A M de Vos
  • J P Merryweather
  • G Kuo
  • P J Barr
  • L S Cousens

Detail Information

Publications36

  1. pmc A method to determine 18 O kinetic isotope effects in the hydrolysis of nucleotide triphosphates
    Xinlin Du
    Department of Biochemistry, University of Texas, Southwestern Medical Center, Dallas, TX 75390, USA
    Anal Biochem 372:213-21. 2008
    ..1%, although the method using the ratio of isotope ratios of GDP and GTP gives superior precision (<0.1%). A single KIE measurement can be conducted in 25 min with less than 5 microg nucleotide reaction product...
  2. ncbi request reprint G proteins, effectors and GAPs: structure and mechanism
    S R Sprang
    Howard Hughes Medical Institute, University of Texas, Southwestern Medical Center, Dallas 75235 9050, USA
    Curr Opin Struct Biol 7:849-56. 1997
    ....
  3. ncbi request reprint G protein mechanisms: insights from structural analysis
    S R Sprang
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Annu Rev Biochem 66:639-78. 1997
    ..G protein-coupled receptors are expected to operate by a somewhat different mechanism, given that the GDP-bound form of many G protein alpha subunits does not contain bound Mg2+...
  4. ncbi request reprint Structure of the GDP-Pi complex of Gly203-->Ala gialpha1: a mimic of the ternary product complex of galpha-catalyzed GTP hydrolysis
    A M Berghuis
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75235 9050, USA
    Structure 4:1277-90. 1996
    ..The structure of the Gly203-->Ala mutant of Gialpha1 (G203AGialpha1) bound to the slowly hydrolyzing analog of GTP (GTPgammaS) has been determined in order to elucidate the structural changes that take place during hydrolysis...
  5. ncbi request reprint Two-metal-Ion catalysis in adenylyl cyclase
    J J Tesmer
    Howard Hughes Medical Institute, Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9050, USA
    Science 285:756-60. 1999
    ..The similarity of the active site of AC to those of DNA polymerases suggests that the enzymes catalyze phosphoryl transfer by the same two-metal-ion mechanism and likely have evolved from a common ancestor...
  6. ncbi request reprint Structure of RGS4 bound to AlF4--activated G(i alpha1): stabilization of the transition state for GTP hydrolysis
    J J Tesmer
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 89:251-61. 1997
    ..The binding site for RGS4 on G(i alpha1) is also consistent with the activity of RGS proteins as antagonists of G(alpha) effectors...
  7. ncbi request reprint Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS
    J J Tesmer
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75235 9050, USA
    Science 278:1907-16. 1997
    ..On the basis of these and other structures, a molecular mechanism is proposed for the activation of adenylyl cyclase by Gsalpha...
  8. ncbi request reprint The A326S mutant of Gialpha1 as an approximation of the receptor-bound state
    B A Posner
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 273:21752-8. 1998
    ....
  9. ncbi request reprint Crystal structure of the adenylyl cyclase activator Gsalpha
    R K Sunahara
    Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9041, USA
    Science 278:1943-7. 1997
    ....
  10. ncbi request reprint Structures of active conformations of Gi alpha 1 and the mechanism of GTP hydrolysis
    D E Coleman
    Howard Hughes Medical Institute, Dallas, TX
    Science 265:1405-12. 1994
    ..The amino-terminal 33 residues are disordered in GTP gamma S-Gi alpha 1, suggesting a mechanism that may promote release of the beta gamma subunit complex when the alpha subunit is activated by GTP...
  11. ncbi request reprint Crystal structures of the G protein Gi alpha 1 complexed with GDP and Mg2+: a crystallographic titration experiment
    D E Coleman
    Howard Hughes Medical Institute, Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Biochemistry 37:14376-85. 1998
    ..Mg2+ binding also induces binding of an SO42- molecule to the active site in a manner which may mimic a Gi alpha 1.GDP.PO42-.Mg2+ product complex. Implications of these findings are discussed...
  12. ncbi request reprint Identification of a Gialpha binding site on type V adenylyl cyclase
    C W Dessauer
    Department of Pharmacology, Dallas, Texas 75235, USA
    J Biol Chem 273:25831-9. 1998
    ..The pseudosymmetrical structure of adenylyl cyclase permits bidirectional regulation of activity by homologous G protein alpha subunits...
  13. ncbi request reprint The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2
    M A Wall
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235, USA
    Cell 83:1047-58. 1995
    ..Repeated WD motifs in beta form a circularized sevenfold beta propeller. The conserved cores of these motifs are a scaffold for display of their more variable linkers on the exterior face of each propeller blade...
  14. ncbi request reprint Three-dimensional structure of a complex between the death domains of Pelle and Tube
    T Xiao
    The Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas 75235 9050, USA
    Cell 99:545-55. 1999
    ..In vivo assays of Pelle and Tube mutants confirmed that the integrity of the major heterodimer interface is critical to the activity of these molecules...
  15. ncbi request reprint Structural basis for the activation of glycogen phosphorylase b by adenosine monophosphate
    S R Sprang
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050
    Science 254:1367-71. 1991
    ..The structure also reveals previously unobserved interactions with the nucleotide that accounts for the specificity of the nucleotide binding site for AMP in preference to inosine monophosphate...
  16. ncbi request reprint Structure of the rgRGS domain of p115RhoGEF
    Z Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390 9050, USA
    Nat Struct Biol 8:805-9. 2001
    ..Mutagenesis experiments show that rgRGS may form interactions with G alpha(13) that are analogous to those in complexes of RGS proteins with their G alpha substrates...
  17. ncbi request reprint The structure, catalytic mechanism and regulation of adenylyl cyclase
    J J Tesmer
    Howard Hughes Medical Institute Department of Biochemistry University of Texas Southwestern Medical Center 5323 Harry Hines Boulevard Dallas TX 75235 9050 USA
    Curr Opin Struct Biol 8:713-9. 1998
    ....
  18. ncbi request reprint Crystallization of trimeric recombinant human tumor necrosis factor (cachectin)
    M J Eck
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235 9050
    J Biol Chem 263:12816-9. 1988
    ..08, c = 117.49. The asymmetric unit contains one trimer; the crystals are stable in the x-ray beam and diffract to beyond 3 A...
  19. ncbi request reprint Structure of the protein kinase Cbeta phospholipid-binding C2 domain complexed with Ca2+
    R B Sutton
    Howard Hughes Medical Institute Department of Biochemistry The University of Texas Southwestern Medical Center 5323 Harry Hines Blvd Dallas, TX 75235 9050, USA
    Structure 6:1395-405. 1998
    ..Neither the structural basis for cooperativity between phosphatidylserine and Ca2+, nor the binding site for phosphatidylserine are known...
  20. ncbi request reprint Structures, mechanism, regulation and evolution of class III nucleotidyl cyclases
    S C Sinha
    University of Texas Southwestern Medical Center, Division of Infectious Diseases, Department of Internal Medicine, 5323 Harry Hines Blvd, Dallas 75390 9113, USA
    Rev Physiol Biochem Pharmacol 157:105-40. 2006
    ..Many of these regulators act by altering the interface of the catalytic domains and therefore the integrity of the catalytic site(s). This review focuses on both conserved and divergent mechanisms of class III NC function and regulation...
  21. ncbi request reprint Conformational display: a role for switch polymorphism in the superfamily of regulatory GTPases
    S R Sprang
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA
    Sci STKE 2000:pe1. 2000
    ..Thus, rather than a simplistic on/off two-conformation model, these signaling proteins exist in several differing conformations that may be related to activation and effector protein binding...
  22. pmc Three-dimensional structure of human basic fibroblast growth factor, a structural homolog of interleukin 1 beta
    J D Zhang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235 9050
    Proc Natl Acad Sci U S A 88:3446-50. 1991
    ..Analysis of the three-dimensional structure in light of functional studies of bFGF suggests that the receptor binding site and the positively charged heparin binding site correspond to adjacent but separate loci on the beta-barrel...
  23. ncbi request reprint The structure of human lymphotoxin (tumor necrosis factor-beta) at 1.9-A resolution
    M J Eck
    Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9050
    J Biol Chem 267:2119-22. 1992
    ....
  24. ncbi request reprint Mapping the Galpha13 binding interface of the rgRGS domain of p115RhoGEF
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:9912-9. 2003
    ..This suggests that the rgRGS domain may serve a structural or allosteric role in the regulation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13)...
  25. pmc Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit
    Celestine J Thomas
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 101:7560-5. 2004
    ..In (K180P)G alpha(i1), the two events are decoupled kinetically, whereas in the native protein they are concerted...
  26. ncbi request reprint Structural basis for the inhibition of mammalian membrane adenylyl cyclase by 2 '(3')-O-(N-Methylanthraniloyl)-guanosine 5 '-triphosphate
    Tung Chung Mou
    Department of Biochemistry, Howard Hughes Medical Institute, The University of Texas Southwestern Medical, Dallas, Texas 75390 9050, USA
    J Biol Chem 280:7253-61. 2005
    ..Mutational analysis of two non-conserved amino acids in the MANT-binding pocket suggests that residues outside of the binding site influence isoform selectivity toward MANT-GTP...
  27. ncbi request reprint A peptide core motif for binding to heterotrimeric G protein alpha subunits
    William W Ja
    Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, 91125, USA
    J Biol Chem 280:32057-60. 2005
    ....
  28. ncbi request reprint Distinct interactions of GTP, UTP, and CTP with G(s) proteins
    Andreas Gille
    Department of Pharmacology and Toxicology, University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045 7582, USA
    J Biol Chem 277:34434-42. 2002
    ..G protein activation by UTP and CTP could be of particular importance in pathological conditions such as cholera and Lesch-Nyhan syndrome...
  29. ncbi request reprint Structural basis of effector regulation and signal termination in heterotrimeric Galpha proteins
    Stephen R Sprang
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Adv Protein Chem 74:1-65. 2007
    ..Unlike GAPs for small GTPases, Galpha GAPs supply no catalytic residues, but rather appear to reduce the activation energy for catalytic activation of the Galpha catalytic site...
  30. ncbi request reprint Structural and molecular characterization of a preferred protein interaction surface on G protein beta gamma subunits
    Tara L Davis
    Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, MC 9050, Dallas, Texas 75390 9050, USA
    Biochemistry 44:10593-604. 2005
    ....
  31. pmc Origin of asymmetry in adenylyl cyclases: structures of Mycobacterium tuberculosis Rv1900c
    Sangita C Sinha
    Howard Hughes Medical Institute and Department of Biochemistry, The University of Texas Southwestern Medical Center, Dallas, TX, USA
    EMBO J 24:663-73. 2005
    ..Such a mechanism of half-of-sites-reactivity suggests that mammalian heterodimeric adenylyl cyclases may have evolved from gene duplication of a primitive prokaryote-type cyclase, followed by loss of function in one active site...
  32. ncbi request reprint Structure of the p115RhoGEF rgRGS domain-Galpha13/i1 chimera complex suggests convergent evolution of a GTPase activator
    Zhe Chen
    Department of Biochemistry, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75390, USA
    Nat Struct Mol Biol 12:191-7. 2005
    ..The interface between the RGS module of rgRGS and Galpha is similar to that of a Galpha-effector complex, suggesting a role for the rgRGS domain in the stimulation of the GEF activity of p115RhoGEF by Galpha13...
  33. pmc Kinetic isotope effects in Ras-catalyzed GTP hydrolysis: evidence for a loose transition state
    Xinlin Du
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Proc Natl Acad Sci U S A 101:8858-63. 2004
    ..The KIE effects, interpreted in light of structural and spectroscopic data, suggest that Ras promotes a loose transition state by stabilizing negative charge in the beta-gamma bridge and beta nonbridge oxygens of GTP...
  34. ncbi request reprint Thermodynamic characterization of the binding of activator of G protein signaling 3 (AGS3) and peptides derived from AGS3 with G alpha i1
    Anirban Adhikari
    Department of Biochemistry and Molecular Biophysics Graduate Program, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    J Biol Chem 278:51825-32. 2003
    ....
  35. pmc Cosolvent-induced transformation of a death domain tertiary structure
    Tsan Xiao
    The Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 99:11151-6. 2002
    ..Generally, crystallographers should be aware that high concentrations of MPD or related cosolvents can alter the tertiary structure of susceptible proteins...
  36. ncbi request reprint Structural biology: a receptor unlocked
    Stephen R Sprang
    Nature 450:355-6. 2007