Research Topics
| ARTHUR SPECTORSummaryAffiliation: University of Iowa Country: USA Publications
Research Grants
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Detail Information
Publications
Essentiality of fatty acidsA A Spector
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Lipids 34:S1-3. 1999..Thus, the essentiality of n-3 PUFA may be due to their ability to supply enough 22-carbon PUFA for optimal membrane function rather than to a unique biochemical property of DHA...- Epoxyeicosatrienoic acids (EETs): metabolism and biochemical functionArthur A Spector
Department of Biochemistry, 4 403 Bowen Science Building, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Prog Lipid Res 43:55-90. 2004..Because of the diversity of biochemical and functional responses produced by EETs, it is doubtful that a single mechanism or signal transduction pathway can account for all of their actions... - Action of epoxyeicosatrienoic acids on cellular functionArthur A Spector
Dept of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
Am J Physiol Cell Physiol 292:C996-1012. 2007..Therefore, sEH is considered a potential therapeutic target for enhancing the beneficial functions of EETs... 
Arachidonic acid cytochrome P450 epoxygenase pathwayArthur A Spector
Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
J Lipid Res 50:S52-6. 2009..Because this attenuates many of the functional effects of EETs, sEH inhibition is being evaluated as a mechanism for increasing and prolonging the beneficial actions of EETs...- Plasma free fatty acid and lipoproteins as sources of polyunsaturated fatty acid for the brainA A Spector
Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
J Mol Neurosci 16:159-65; discussion 215-21. 2001..Alternatively, if the brain requires large amounts of preformed DHA, the phospholipids contained in plasma lipoproteins are the most likely source... - Role of peroxisomal oxidation in the conversion of arachidonic acid to eicosatrienoic acid in human skin fibroblastsA A Spector
Department of Biochemistry, University of Iowa, Iowa City 52242, USA
Prostaglandins Leukot Essent Fatty Acids 60:377-82. 1999.... - Omega-oxidation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4Xixuan H Collins
Department of Biochemistry, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 280:33157-64. 2005..Although ADH4 and ADH3 are expressed in MSMC and MEC, the inhibition produced by N-heptylformamide suggests that ADH4 is primarily responsible for 20-COOH-AA formation in the cerebral microvasculature... - Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid productionShawn D Harmon
Department of Biochemistry, Carver College of Medicine, 4-403 BSB, University of Iowa, Iowa City, IA 52242, USA
Prostaglandins Leukot Essent Fatty Acids 75:169-77. 2006..These findings suggest that some functional effects of omega-3 fatty acid supplementation may be due to inhibition of 20-HETE formation or the conversion of EPA to the corresponding omega-oxidized product... - 20-carboxy-arachidonic acid is a dual activator of peroxisome proliferator-activated receptors alpha and gammaXiang Fang
Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States
Prostaglandins Other Lipid Mediat 82:175-84. 2007..87+/-0.12 microM) and PPARgamma (Kd=1.7+/-0.5 microM). These findings suggest that 20-COOH-AA, a relatively stable metabolite of 20-HETE, might function as an endogenous dual activator of PPARalpha and PPARgamma... - Metabolism of anandamide in cerebral microvascular endothelial cellsPing Chen
Department of Biochemistry, University of Iowa College of Medicine, 4-403 BSB, Iowa City, IA 52242, USA
Prostaglandins Other Lipid Mediat 73:59-72. 2004.... - Effect of soluble epoxide hydrolase inhibition on epoxyeicosatrienoic acid metabolism in human blood vesselsXiang Fang
Dept of Biochemistry, 4 403 BSB, Univ of Iowa College of Medicine, Iowa City, IA 52242, USA
Am J Physiol Heart Circ Physiol 287:H2412-20. 2004..These findings in intact human blood vessels indicate that conversion to DHET is the predominant pathway for 11,12- and 14,15-EET metabolism and that sEH inhibition can modulate EET metabolism in vascular tissue... - Neuroticism but not omega-3 fatty acid levels correlate with early responsiveness to escitalopramJess G Fiedorowicz
Departments of Psychiatry and Epidemiology, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA
Ann Clin Psychiatry 22:157-63. 2010..We sought to study the utility of O3FA levels and dimensional personality measures as predictors of early improvement with escitalopram... - Activation of peroxisome proliferator-activated receptor alpha by substituted urea-derived soluble epoxide hydrolase inhibitorsXiang Fang
Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
J Pharmacol Exp Ther 314:260-70. 2005..Further development of these compounds could lead to a class of agents with hypotensive and lipid-lowering properties that may be valuable for the prevention and treatment of cardiovascular disease... - 14,15-Dihydroxyeicosatrienoic acid activates peroxisome proliferator-activated receptor-alphaXiang Fang
Dept of Biochemistry, Univ of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
Am J Physiol Heart Circ Physiol 290:H55-63. 2006..These findings suggest that 14,15-DHET, produced from 14,15-EET by the action of SEH, may function as an endogenous activator of PPARalpha... - 20-hydroxyeicosatetraenoic acid (20-HETE) metabolism in coronary endothelial cellsTerry L Kaduce
Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
J Biol Chem 279:2648-56. 2004..These results suggest that the incorporation of 20-HETE and its subsequent conversion to 20-COOH-AA in the endothelium may be important in modulating coronary vascular function... - Fatty acid-binding proteins inhibit hydration of epoxyeicosatrienoic acids by soluble epoxide hydrolaseRichard L Widstrom
Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, Iowa 52242, USA
Biochemistry 42:11762-7. 2003..However, the effectiveness of this process may depend on metabolic conditions that regulate the levels of competing FABP ligands... - Human coronary endothelial cells convert 14,15-EET to a biologically active chain-shortened epoxideXiang Fang
Department of Biochemistry, University of Iowa, Iowa City 52242, USA
Am J Physiol Heart Circ Physiol 283:H2306-14. 2002..These findings implicate beta-oxidation as a major pathway of 14,15-EET metabolism in HCEC and provide the first evidence that EET-derived chain-shortened epoxy fatty acids are biologically active... - Evidence for a membrane site of action for 14,15-EET on expression of aromatase in vascular smooth muscleGary D Snyder
Department of Biochemistry, 4 403 BSB, University of Iowa, Iowa City, IA 52242, USA
Am J Physiol Heart Circ Physiol 283:H1936-42. 2002.... - Reactive oxygen species mediate arachidonic acid-induced dilation in porcine coronary microvesselsChristine L Oltman
Department of Internal Medicine and the Cardiovascular Research Center, University of Iowa and Department of Veterans Affairs Medical Center, Iowa City, 52246, USA
Am J Physiol Heart Circ Physiol 285:H2309-15. 2003..We conclude that in porcine coronary microvessels, COX-derived ROS contribute to AA-induced vasodilation... - Differences in positional esterification of 14,15-epoxyeicosatrienoic acid in phosphatidylcholine of porcine coronary artery endothelial and smooth muscle cellsXiang Fang
Department of Biochemistry, University of Iowa College of Medicine, 4 403 BSB, Iowa City, IA 52242, USA
Prostaglandins Other Lipid Mediat 71:33-42. 2003.... - Effect of the delta6-desaturase inhibitor SC-26196 on PUFA metabolism in human cellsShawn D Harmon
Departmentof Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
Lipids 38:469-76. 2003..Furthermore, they provide additional evidence that the conversion of 22:5n-3 to DHA involves delta6-desaturation... - Very long chain n-3 and n-6 polyunsaturated fatty acids bind strongly to liver fatty acid-binding proteinAndrew W Norris
Department of Pediatrics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
J Lipid Res 43:646-53. 2002.... - Activation of NAD(P)H oxidase by lipid hydroperoxides: mechanism of oxidant-mediated smooth muscle cytotoxicityWei-Gen Li
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA 52242, USA
Free Radic Biol Med 34:937-46. 2003..These findings suggest that activation of NAD(P)H oxidase, and production of O2*-, play an important role in lipid hydroperoxide-induced smooth muscle cytotoxicity... - Induction of cyclooxygenase-2 by anandamide in cerebral microvascular endotheliumPing Chen
Department of Biochemistry, 4-403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
Microvasc Res 69:28-35. 2005..These results indicate that AEA increased COX-2 expression at the transcriptional level through, at least in part, a cannabinoid receptor-1-mediated mechanism in cerebral microvascular endothelium... - Characterization of transgenic mice with neuron-specific expression of soluble epoxide hydrolaseRobert A Bianco
Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USA
Brain Res 1291:60-72. 2009..Despite a 3-fold increase in sEH activity in the brain, there was no change in arterial pressure. This data provides new information required for studying the central roles of the cytochrome P450 epoxygenase pathway... - 20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenaseXiang Fang
Dept of Medicine, Harbor Hospital Center, 3001 S Hanover St, Baltimore MD 21225, USA
Am J Physiol Heart Circ Physiol 291:H2301-7. 2006..Whereas the conversion of 20-HETE to 20-OH-PGE(2) and production of endogenous prostaglandins probably are primarily responsible for vasodilation, the production of 20-COOH-AA also may contribute to this process... - The antiinflammatory effect of laminar flow: the role of PPARgamma, epoxyeicosatrienoic acids, and soluble epoxide hydrolaseYi Liu
Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
Proc Natl Acad Sci U S A 102:16747-52. 2005..Blocking PPARgamma by GW9662 abolished the EET/AUDA-mediated antiinflammatory effect, which indicates that PPARgamma is an effector of EETs... - Inhibition of PGI2 signaling by miconazole in vascular smooth muscle cellsWei Zhou
Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, United States
Prostaglandins Other Lipid Mediat 80:28-34. 2006..These results indicate that in addition to its CYP inhibition properties, miconazole inhibits PGI(2) signaling. Hence, experiments using miconazole as a CYP inhibitor should be interpreted with caution... - LDL cholesteryl oleate: a biomarker for atherosclerosis?Arthur A Spector
Arterioscler Thromb Vasc Biol 27:1228-30. 2007 - Impaired arachidonic acid-mediated activation of large-conductance Ca2+-activated K+ channels in coronary arterial smooth muscle cells in Zucker Diabetic Fatty ratsTong Lu
Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St, SW, Rochester, MN 55905, USA
Diabetes 54:2155-63. 2005..We conclude that PGI2 is the major AA metabolite in lean coronaries, and AA-mediated BK channel activation is impaired in ZDF coronaries due to reduced PGIS activity... - Plaque rupture, lysophosphatidic acid, and thrombosisArthur A Spector
Circulation 108:641-3. 2003 - Impaired arachidonic acid-mediated dilation of small mesenteric arteries in Zucker diabetic fatty ratsWei Zhou
Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
Am J Physiol Heart Circ Physiol 288:H2210-8. 2005..Increased oxidative stress and nitration of 12-lipoxygenase may underlie the impairment of AA-mediated relaxation in small mesenteric arteries of diabetic rats...
Research Grants
- Biochemical and Metabolic Properties of 10-HETEARTHUR SPECTOR; Fiscal Year: 2006..The ultimate goal of this project is to provide the additional mechanistic insight necessary to develop new therapeutic approaches to overcome the pathological actions of 20-HETE. ..