ARTHUR SPECTOR

Summary

Affiliation: University of Iowa
Country: USA

Publications

  1. ncbi request reprint Essentiality of fatty acids
    A A Spector
    Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
    Lipids 34:S1-3. 1999
  2. ncbi request reprint Epoxyeicosatrienoic acids (EETs): metabolism and biochemical function
    Arthur A Spector
    Department of Biochemistry, 4 403 Bowen Science Building, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Prog Lipid Res 43:55-90. 2004
  3. ncbi request reprint Action of epoxyeicosatrienoic acids on cellular function
    Arthur A Spector
    Dept of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Am J Physiol Cell Physiol 292:C996-1012. 2007
  4. pmc Arachidonic acid cytochrome P450 epoxygenase pathway
    Arthur A Spector
    Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    J Lipid Res 50:S52-6. 2009
  5. ncbi request reprint Plasma free fatty acid and lipoproteins as sources of polyunsaturated fatty acid for the brain
    A A Spector
    Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
    J Mol Neurosci 16:159-65; discussion 215-21. 2001
  6. ncbi request reprint Role of peroxisomal oxidation in the conversion of arachidonic acid to eicosatrienoic acid in human skin fibroblasts
    A A Spector
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Prostaglandins Leukot Essent Fatty Acids 60:377-82. 1999
  7. ncbi request reprint Omega-oxidation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4
    Xixuan H Collins
    Department of Biochemistry, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 280:33157-64. 2005
  8. ncbi request reprint Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production
    Shawn D Harmon
    Department of Biochemistry, Carver College of Medicine, 4 403 BSB, University of Iowa, Iowa City, IA 52242, USA
    Prostaglandins Leukot Essent Fatty Acids 75:169-77. 2006
  9. ncbi request reprint 20-carboxy-arachidonic acid is a dual activator of peroxisome proliferator-activated receptors alpha and gamma
    Xiang Fang
    Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States
    Prostaglandins Other Lipid Mediat 82:175-84. 2007
  10. ncbi request reprint Metabolism of anandamide in cerebral microvascular endothelial cells
    Ping Chen
    Department of Biochemistry, University of Iowa College of Medicine, 4 403 BSB, Iowa City, IA 52242, USA
    Prostaglandins Other Lipid Mediat 73:59-72. 2004

Research Grants

  1. Biochemical and Metabolic Properties of 10-HETE
    ARTHUR SPECTOR; Fiscal Year: 2006

Collaborators

Detail Information

Publications32

  1. ncbi request reprint Essentiality of fatty acids
    A A Spector
    Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
    Lipids 34:S1-3. 1999
    ..Thus, the essentiality of n-3 PUFA may be due to their ability to supply enough 22-carbon PUFA for optimal membrane function rather than to a unique biochemical property of DHA...
  2. ncbi request reprint Epoxyeicosatrienoic acids (EETs): metabolism and biochemical function
    Arthur A Spector
    Department of Biochemistry, 4 403 Bowen Science Building, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    Prog Lipid Res 43:55-90. 2004
    ..Because of the diversity of biochemical and functional responses produced by EETs, it is doubtful that a single mechanism or signal transduction pathway can account for all of their actions...
  3. ncbi request reprint Action of epoxyeicosatrienoic acids on cellular function
    Arthur A Spector
    Dept of Biochemistry, University of Iowa, Iowa City, IA 52242, USA
    Am J Physiol Cell Physiol 292:C996-1012. 2007
    ..Therefore, sEH is considered a potential therapeutic target for enhancing the beneficial functions of EETs...
  4. pmc Arachidonic acid cytochrome P450 epoxygenase pathway
    Arthur A Spector
    Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
    J Lipid Res 50:S52-6. 2009
    ..Because this attenuates many of the functional effects of EETs, sEH inhibition is being evaluated as a mechanism for increasing and prolonging the beneficial actions of EETs...
  5. ncbi request reprint Plasma free fatty acid and lipoproteins as sources of polyunsaturated fatty acid for the brain
    A A Spector
    Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
    J Mol Neurosci 16:159-65; discussion 215-21. 2001
    ..Alternatively, if the brain requires large amounts of preformed DHA, the phospholipids contained in plasma lipoproteins are the most likely source...
  6. ncbi request reprint Role of peroxisomal oxidation in the conversion of arachidonic acid to eicosatrienoic acid in human skin fibroblasts
    A A Spector
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Prostaglandins Leukot Essent Fatty Acids 60:377-82. 1999
    ....
  7. ncbi request reprint Omega-oxidation of 20-hydroxyeicosatetraenoic acid (20-HETE) in cerebral microvascular smooth muscle and endothelium by alcohol dehydrogenase 4
    Xixuan H Collins
    Department of Biochemistry, Carver College of Medicine, The University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 280:33157-64. 2005
    ..Although ADH4 and ADH3 are expressed in MSMC and MEC, the inhibition produced by N-heptylformamide suggests that ADH4 is primarily responsible for 20-COOH-AA formation in the cerebral microvasculature...
  8. ncbi request reprint Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production
    Shawn D Harmon
    Department of Biochemistry, Carver College of Medicine, 4 403 BSB, University of Iowa, Iowa City, IA 52242, USA
    Prostaglandins Leukot Essent Fatty Acids 75:169-77. 2006
    ..These findings suggest that some functional effects of omega-3 fatty acid supplementation may be due to inhibition of 20-HETE formation or the conversion of EPA to the corresponding omega-oxidized product...
  9. ncbi request reprint 20-carboxy-arachidonic acid is a dual activator of peroxisome proliferator-activated receptors alpha and gamma
    Xiang Fang
    Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, United States
    Prostaglandins Other Lipid Mediat 82:175-84. 2007
    ..87+/-0.12 microM) and PPARgamma (Kd=1.7+/-0.5 microM). These findings suggest that 20-COOH-AA, a relatively stable metabolite of 20-HETE, might function as an endogenous dual activator of PPARalpha and PPARgamma...
  10. ncbi request reprint Metabolism of anandamide in cerebral microvascular endothelial cells
    Ping Chen
    Department of Biochemistry, University of Iowa College of Medicine, 4 403 BSB, Iowa City, IA 52242, USA
    Prostaglandins Other Lipid Mediat 73:59-72. 2004
    ....
  11. ncbi request reprint Effect of soluble epoxide hydrolase inhibition on epoxyeicosatrienoic acid metabolism in human blood vessels
    Xiang Fang
    Dept of Biochemistry, 4 403 BSB, Univ of Iowa College of Medicine, Iowa City, IA 52242, USA
    Am J Physiol Heart Circ Physiol 287:H2412-20. 2004
    ..These findings in intact human blood vessels indicate that conversion to DHET is the predominant pathway for 11,12- and 14,15-EET metabolism and that sEH inhibition can modulate EET metabolism in vascular tissue...
  12. pmc Neuroticism but not omega-3 fatty acid levels correlate with early responsiveness to escitalopram
    Jess G Fiedorowicz
    Departments of Psychiatry and Epidemiology, Carver College of Medicine, The University of Iowa, Iowa City, IA, USA
    Ann Clin Psychiatry 22:157-63. 2010
    ..We sought to study the utility of O3FA levels and dimensional personality measures as predictors of early improvement with escitalopram...
  13. pmc Activation of peroxisome proliferator-activated receptor alpha by substituted urea-derived soluble epoxide hydrolase inhibitors
    Xiang Fang
    Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    J Pharmacol Exp Ther 314:260-70. 2005
    ..Further development of these compounds could lead to a class of agents with hypotensive and lipid-lowering properties that may be valuable for the prevention and treatment of cardiovascular disease...
  14. ncbi request reprint 14,15-Dihydroxyeicosatrienoic acid activates peroxisome proliferator-activated receptor-alpha
    Xiang Fang
    Dept of Biochemistry, Univ of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Am J Physiol Heart Circ Physiol 290:H55-63. 2006
    ..These findings suggest that 14,15-DHET, produced from 14,15-EET by the action of SEH, may function as an endogenous activator of PPARalpha...
  15. ncbi request reprint 20-hydroxyeicosatetraenoic acid (20-HETE) metabolism in coronary endothelial cells
    Terry L Kaduce
    Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA
    J Biol Chem 279:2648-56. 2004
    ..These results suggest that the incorporation of 20-HETE and its subsequent conversion to 20-COOH-AA in the endothelium may be important in modulating coronary vascular function...
  16. ncbi request reprint Fatty acid-binding proteins inhibit hydration of epoxyeicosatrienoic acids by soluble epoxide hydrolase
    Richard L Widstrom
    Department of Biochemistry, University of Iowa, College of Medicine, Iowa City, Iowa 52242, USA
    Biochemistry 42:11762-7. 2003
    ..However, the effectiveness of this process may depend on metabolic conditions that regulate the levels of competing FABP ligands...
  17. ncbi request reprint Human coronary endothelial cells convert 14,15-EET to a biologically active chain-shortened epoxide
    Xiang Fang
    Department of Biochemistry, University of Iowa, Iowa City 52242, USA
    Am J Physiol Heart Circ Physiol 283:H2306-14. 2002
    ..These findings implicate beta-oxidation as a major pathway of 14,15-EET metabolism in HCEC and provide the first evidence that EET-derived chain-shortened epoxy fatty acids are biologically active...
  18. ncbi request reprint Evidence for a membrane site of action for 14,15-EET on expression of aromatase in vascular smooth muscle
    Gary D Snyder
    Department of Biochemistry, 4 403 BSB, University of Iowa, Iowa City, IA 52242, USA
    Am J Physiol Heart Circ Physiol 283:H1936-42. 2002
    ....
  19. ncbi request reprint Reactive oxygen species mediate arachidonic acid-induced dilation in porcine coronary microvessels
    Christine L Oltman
    Department of Internal Medicine and the Cardiovascular Research Center, University of Iowa and Department of Veterans Affairs Medical Center, Iowa City, 52246, USA
    Am J Physiol Heart Circ Physiol 285:H2309-15. 2003
    ..We conclude that in porcine coronary microvessels, COX-derived ROS contribute to AA-induced vasodilation...
  20. ncbi request reprint Differences in positional esterification of 14,15-epoxyeicosatrienoic acid in phosphatidylcholine of porcine coronary artery endothelial and smooth muscle cells
    Xiang Fang
    Department of Biochemistry, University of Iowa College of Medicine, 4 403 BSB, Iowa City, IA 52242, USA
    Prostaglandins Other Lipid Mediat 71:33-42. 2003
    ....
  21. ncbi request reprint Effect of the delta6-desaturase inhibitor SC-26196 on PUFA metabolism in human cells
    Shawn D Harmon
    Departmentof Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
    Lipids 38:469-76. 2003
    ..Furthermore, they provide additional evidence that the conversion of 22:5n-3 to DHA involves delta6-desaturation...
  22. ncbi request reprint Very long chain n-3 and n-6 polyunsaturated fatty acids bind strongly to liver fatty acid-binding protein
    Andrew W Norris
    Department of Pediatrics, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    J Lipid Res 43:646-53. 2002
    ....
  23. ncbi request reprint Activation of NAD(P)H oxidase by lipid hydroperoxides: mechanism of oxidant-mediated smooth muscle cytotoxicity
    Wei Gen Li
    Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA 52242, USA
    Free Radic Biol Med 34:937-46. 2003
    ..These findings suggest that activation of NAD(P)H oxidase, and production of O2*-, play an important role in lipid hydroperoxide-induced smooth muscle cytotoxicity...
  24. ncbi request reprint Induction of cyclooxygenase-2 by anandamide in cerebral microvascular endothelium
    Ping Chen
    Department of Biochemistry, 4 403 BSB, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA
    Microvasc Res 69:28-35. 2005
    ..These results indicate that AEA increased COX-2 expression at the transcriptional level through, at least in part, a cannabinoid receptor-1-mediated mechanism in cerebral microvascular endothelium...
  25. pmc Characterization of transgenic mice with neuron-specific expression of soluble epoxide hydrolase
    Robert A Bianco
    Department of Internal Medicine, The University of Iowa, Iowa City, IA 52242, USA
    Brain Res 1291:60-72. 2009
    ..Despite a 3-fold increase in sEH activity in the brain, there was no change in arterial pressure. This data provides new information required for studying the central roles of the cytochrome P450 epoxygenase pathway...
  26. ncbi request reprint 20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenase
    Xiang Fang
    Dept of Medicine, Harbor Hospital Center, 3001 S Hanover St, Baltimore MD 21225, USA
    Am J Physiol Heart Circ Physiol 291:H2301-7. 2006
    ..Whereas the conversion of 20-HETE to 20-OH-PGE(2) and production of endogenous prostaglandins probably are primarily responsible for vasodilation, the production of 20-COOH-AA also may contribute to this process...
  27. pmc The antiinflammatory effect of laminar flow: the role of PPARgamma, epoxyeicosatrienoic acids, and soluble epoxide hydrolase
    Yi Liu
    Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 102:16747-52. 2005
    ..Blocking PPARgamma by GW9662 abolished the EET/AUDA-mediated antiinflammatory effect, which indicates that PPARgamma is an effector of EETs...
  28. ncbi request reprint Inhibition of PGI2 signaling by miconazole in vascular smooth muscle cells
    Wei Zhou
    Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, United States
    Prostaglandins Other Lipid Mediat 80:28-34. 2006
    ..These results indicate that in addition to its CYP inhibition properties, miconazole inhibits PGI(2) signaling. Hence, experiments using miconazole as a CYP inhibitor should be interpreted with caution...
  29. ncbi request reprint LDL cholesteryl oleate: a biomarker for atherosclerosis?
    Arthur A Spector
    Arterioscler Thromb Vasc Biol 27:1228-30. 2007
  30. ncbi request reprint Impaired arachidonic acid-mediated activation of large-conductance Ca2+-activated K+ channels in coronary arterial smooth muscle cells in Zucker Diabetic Fatty rats
    Tong Lu
    Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St, SW, Rochester, MN 55905, USA
    Diabetes 54:2155-63. 2005
    ..We conclude that PGI2 is the major AA metabolite in lean coronaries, and AA-mediated BK channel activation is impaired in ZDF coronaries due to reduced PGIS activity...
  31. ncbi request reprint Plaque rupture, lysophosphatidic acid, and thrombosis
    Arthur A Spector
    Circulation 108:641-3. 2003
  32. ncbi request reprint Impaired arachidonic acid-mediated dilation of small mesenteric arteries in Zucker diabetic fatty rats
    Wei Zhou
    Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    Am J Physiol Heart Circ Physiol 288:H2210-8. 2005
    ..Increased oxidative stress and nitration of 12-lipoxygenase may underlie the impairment of AA-mediated relaxation in small mesenteric arteries of diabetic rats...

Research Grants4

  1. Biochemical and Metabolic Properties of 10-HETE
    ARTHUR SPECTOR; Fiscal Year: 2006
    ..The ultimate goal of this project is to provide the additional mechanistic insight necessary to develop new therapeutic approaches to overcome the pathological actions of 20-HETE. ..