A P Somlyo

Summary

Affiliation: University of Virginia
Country: USA

Publications

  1. ncbi Cooperativity between the Ras-ERK and Rho-Rho kinase pathways in urokinase-type plasminogen activator-stimulated cell migration
    Minji Jo
    Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:12479-85. 2002
  2. ncbi Myosin light chain kinase knockout
    A V Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA
    J Muscle Res Cell Motil 25:241-2. 2004
  3. ncbi Smooth muscle differentiation marker gene expression is regulated by RhoA-mediated actin polymerization
    C P Mack
    Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:341-7. 2001
  4. ncbi Site-specific phosphorylation and point mutations of telokin modulate its Ca2+-desensitizing effect in smooth muscle
    L A Walker
    Department of Molecular Physiology and Biological Physics, Health System, Charlottesville, VA 22908-0736, USA
    J Biol Chem 276:24519-24. 2001
  5. pmc Smooth muscle of telokin-deficient mice exhibits increased sensitivity to Ca2+ and decreased cGMP-induced relaxation
    A S Khromov
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 103:2440-5. 2006
  6. ncbi How RhoGDI binds Rho
    K Longenecker
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906, USA
    Acta Crystallogr D Biol Crystallogr 55:1503-15. 1999
  7. ncbi Regulation by GDI of RhoA/Rho-kinase-induced Ca2+ sensitization of smooth muscle myosin II
    M C Gong
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Am J Physiol Cell Physiol 281:C257-69. 2001
  8. ncbi Rho-kinase inhibitor retards migration and in vivo dissemination of human prostate cancer cells
    A V Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, Virginia 22906, USA
    Biochem Biophys Res Commun 269:652-9. 2000
  9. ncbi Localization of ryanodine receptors in smooth muscle
    R E Lesh
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville 22906 0011, USA
    Circ Res 82:175-85. 1998
  10. pmc Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin
    Alexander S Khromov
    Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Biophys J 86:2318-28. 2004

Collaborators

Detail Information

Publications35

  1. ncbi Cooperativity between the Ras-ERK and Rho-Rho kinase pathways in urokinase-type plasminogen activator-stimulated cell migration
    Minji Jo
    Department of Pathology, University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA
    J Biol Chem 277:12479-85. 2002
    ..This study supports a model in which the Ras-ERK and Rho-Rho kinase pathways cooperate to promote cell migration. Neutralizing either pathway is sufficient to block the response to agents that stimulate cell migration by activating ERK...
  2. ncbi Myosin light chain kinase knockout
    A V Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA
    J Muscle Res Cell Motil 25:241-2. 2004
  3. ncbi Smooth muscle differentiation marker gene expression is regulated by RhoA-mediated actin polymerization
    C P Mack
    Department of Molecular Physiology and Biological Physics, University of Virginia Medical School, Charlottesville, Virginia 22908, USA
    J Biol Chem 276:341-7. 2001
    ..The results also suggest that RhoA signaling may serve as a convergence point for the multiple signaling pathways that regulate SMC differentiation...
  4. ncbi Site-specific phosphorylation and point mutations of telokin modulate its Ca2+-desensitizing effect in smooth muscle
    L A Walker
    Department of Molecular Physiology and Biological Physics, Health System, Charlottesville, VA 22908-0736, USA
    J Biol Chem 276:24519-24. 2001
    ..We conclude that site-specific modification of the N terminus modulates the Ca2+ -desensitizing effect of telokin on force...
  5. pmc Smooth muscle of telokin-deficient mice exhibits increased sensitivity to Ca2+ and decreased cGMP-induced relaxation
    A S Khromov
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Proc Natl Acad Sci U S A 103:2440-5. 2006
    ..Results indicate that a primary role of telokin is to modulate force through increasing MLCP activity and that this effect is further potentiated through phosphorylation by cGMP in telokin-rich smooth tissues...
  6. ncbi How RhoGDI binds Rho
    K Longenecker
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, VA 22906, USA
    Acta Crystallogr D Biol Crystallogr 55:1503-15. 1999
    ..The entrance of the hydrophobic pocket of RhoGDI is 25 A from the last residue in the RhoA model, with its C-terminus oriented to accommodate the geranylgeranyl group without conformational change in RhoA...
  7. ncbi Regulation by GDI of RhoA/Rho-kinase-induced Ca2+ sensitization of smooth muscle myosin II
    M C Gong
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908, USA
    Am J Physiol Cell Physiol 281:C257-69. 2001
    ..RhoA/GDI can precede translocation of RhoA to the membrane; 3) activation of Rho-kinase exposes a hydrophobic domain; and 4) Cdc42 and Rac1 can inhibit Ca2+ sensitization by activated GTP. RhoA...
  8. ncbi Rho-kinase inhibitor retards migration and in vivo dissemination of human prostate cancer cells
    A V Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia Health System, Charlottesville, Virginia 22906, USA
    Biochem Biophys Res Commun 269:652-9. 2000
    ..We conclude that invasiveness of human prostate cancer is facilitated by the Rho/Rho-kinase pathway, and exploration of selective Rho-kinase inhibitors for limiting invasive progress of prostate cancer is warranted...
  9. ncbi Localization of ryanodine receptors in smooth muscle
    R E Lesh
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville 22906 0011, USA
    Circ Res 82:175-85. 1998
    ....
  10. pmc Myosin regulatory light chain phosphorylation and strain modulate adenosine diphosphate release from smooth muscle Myosin
    Alexander S Khromov
    Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908, USA
    Biophys J 86:2318-28. 2004
    ..ADP state(s) and contributes to the high economy of force...
  11. pmc Signal transduction by G-proteins, rho-kinase and protein phosphatase to smooth muscle and non-muscle myosin II
    A P Somlyo
    Departments of Molecular Physiology and Biological Physics, Medicine Cardiology and Pathology, University of Virginia Health System, PO Box 800736, Charlottesville, VA 22908 0736, USA
    J Physiol 522:177-85. 2000
    ....
  12. pmc Photolytic release of MgADP reduces rigor force in smooth muscle
    A S Khromov
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22906, USA
    Biophys J 80:1905-14. 2001
    ....
  13. ncbi Optimizing the window size for multiple least squares fitting of electron energy loss spectra
    R Ho
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    J Microsc 197:46-51. 2000
    ..In the presence of a non-slowly varying background, common in difference spectra, the minimum window size depends on the fine structure of the signal and the background...
  14. ncbi Crossbridge cycle in smooth muscle: kinetics assessed with flash photolysis and fluorescent probes
    Avril Somlyo
    Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908, USA
    J Muscle Res Cell Motil 25:611-2. 2004
  15. ncbi Telokin mediates Ca2+-desensitization through activation of myosin phosphatase in phasic and tonic smooth muscle
    Nandini Choudhury
    Department of Molecular Physiology, University of Virginia, Health Sciences System, Charlottesville, VA 22908 0736, USA
    J Muscle Res Cell Motil 25:657-65. 2004
    ....
  16. ncbi Uncoupling of GPCR and RhoA-induced Ca2+-sensitization of chicken amnion smooth muscle lacking CPI-17
    Andra S Stevenson
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA
    FEBS Lett 578:73-9. 2004
    ..Together, these data suggest a role for CPI-17 upstream of RhoA activation possibly through activation of another PP1 family member targeted by CPI-17...
  17. pmc Thiophosphorylation of myosin light chain increases rigor stiffness of rabbit smooth muscle
    A S Khromov
    Department of Molecular Physiology and Biological Physics, University of Virginia Health Sciences Center, PO Box 10011, Charlottesville, VA 22906 0011, USA
    J Physiol 512:345-50. 1998
    ....
  18. pmc Smooth muscle myosin: regulation and properties
    Avril V Somlyo
    Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, 1300 Jefferson Park Avenue, Charlottesville, VA 22908 0736, USA
    Philos Trans R Soc Lond B Biol Sci 359:1921-30. 2004
    ..RLC thiophosphorylation led to structural changes in smooth muscle cross-bridges consistent with our findings that thiophosphorylation and strain modulate product release...
  19. ncbi Rho kinase and matrix metalloproteinase inhibitors cooperate to inhibit angiogenesis and growth of human prostate cancer xenotransplants
    Avril V Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia 22908 0736, USA
    FASEB J 17:223-34. 2003
    ....
  20. ncbi Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase
    Andrew P Somlyo
    Dept of Molecular Physiology and Biological Physics, Univ of Virginia, PO Box 800736, Charlottesville, VA 22908 0736
    Physiol Rev 83:1325-58. 2003
    ..It is a potentially important therapeutic target and a subject for translational research...
  21. ncbi LPP expression during in vitro smooth muscle differentiation and stent-induced vascular injury
    I Gorenne
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA, USA
    Circ Res 98:378-85. 2006
    ..In conclusion, LPP appears to be a myocardin-, RhoA/ROK-dependent SMC differentiation marker that plays a role in regulating SMC migration...
  22. ncbi LPP, a LIM protein highly expressed in smooth muscle
    Isabelle Gorenne
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Am J Physiol Cell Physiol 285:C674-85. 2003
    ....
  23. ncbi Signal transduction through the RhoA/Rho-kinase pathway in smooth muscle
    Andrew P Somlyo
    Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, USA
    J Muscle Res Cell Motil 25:613-5. 2004
  24. ncbi L-type voltage-gated Ca2+ channels modulate expression of smooth muscle differentiation marker genes via a rho kinase/myocardin/SRF-dependent mechanism
    B R Wamhoff
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    Circ Res 95:406-14. 2004
    ....
  25. pmc Contractile properties and proteins of smooth muscles of a calponin knockout mouse
    J D Matthew
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22906 0011, USA
    J Physiol 529:811-24. 2000
    ..Our results show no detectable role of calponin in the signal transduction of the Ca2+-sensitization pathways in smooth muscle...
  26. ncbi Visualizing filamentous actin on lipid bilayers by atomic force microscopy in solution
    D Shi
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Box 800736, Charlottesville, VA 22908-0736
    J Microsc 201:377-82. 2001
    ..As such specimens are relatively easy to prepare without specialized equipment, this method may allow the study of the thin filaments in which F-actin-associated proteins are also present...
  27. ncbi Improved precision of quantitating calcium in biological electron probe analysis
    R Ho
    Department of Molecular Physiology and Biological Physics, University of Virginia, P O Box 800736, Charlottesville, VA 22908 0736, USA
    J Microsc 204:61-8. 2001
    ..6 for a given electron dose. The influence of non-statistical, instrumental sources of measurement errors is considered, and we show a simple method for reducing the effect of these sources of error in the directly fitted spectra...
  28. ncbi A system for acquiring simultaneous electron energy-loss and X-ray spectrum-images
    J Feng
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville 22908, USA
    J Microsc 215:92-9. 2004
    ..Several advanced features are implemented for real-time and/or off-line quantitative analysis, and the performance of the system is illustrated with practical applications to compositional imaging of cardiac muscle...
  29. ncbi RhoA-mediated Ca2+ sensitization in erectile function
    Hua Wang
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908 0736, USA
    J Biol Chem 277:30614-21. 2002
    ....
  30. ncbi The sarcoplasmic reticulum: then and now
    Andrew P Somlyo
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville 22908 0736, USA
    Novartis Found Symp 246:258-68; discussion 268-71, 272-6. 2002
    ..Electron energy loss spectroscopy for high-resolution Ca2+ imaging and flash photolysis of caged agonists for exploration of the rapid kinetics of Ca2+ release from the SR are currently being explored...
  31. ncbi Calcium content of peripheral and central mitochondria in the guinea pig myocardium: electron probe analysis
    Ruoya Ho
    Department of Molecular Physiology and Biological Physics, University of Virginia, PO Box 800736, Jordan Hall 4 7, Charlottesville, VA 22908 0736, USA
    Cell Calcium 33:247-56. 2003
    ....
  32. ncbi Cryo-atomic force microscopy of unphosphorylated and thiophosphorylated single smooth muscle myosin molecules
    Sitong Sheng
    Department of Molecular Physiology and Biological Physics, University of Virginia School of Medicine, Charlottesville, Virginia 22908 0736, USA
    J Biol Chem 278:39892-6. 2003
    ....
  33. ncbi The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle
    Urszula Derewenda
    Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, VA 22908, USA
    Structure 12:1955-65. 2004
    ..The DH domain makes several specific interactions with RhoA residues not conserved among other Rho family members, suggesting the molecular basis for the observed specificity...
  34. ncbi Intracellular chloride accumulation and subcellular elemental distribution during atrial fibrillation
    Joseph G Akar
    Department of Molecular Physiology and Biological Physics and the Cardiovascular Division, University of Virginia Health Sciences Center, Charlottesville, USA
    Circulation 107:1810-5. 2003
    ..Electron probe microanalysis was used to determine the total (free+bound) in vivo subcellular concentration of these elements during AF...
  35. ncbi Modulation of Ca2+ sensitivity by cyclic nucleotides in smooth muscle from protein kinase G-deficient mice
    Johan Bonnevier
    Department of Physiological Sciences, Martinsried, Munich 82152, Germany
    J Biol Chem 279:5146-51. 2004
    ..Relaxation by cAMP can occur independently of PKG. Long term deficiency of PKG does not lead to an apparent up-regulation of the cAMP-dependent pathways or changes in Ca2+ sensitivity...