Affiliation: University of North Carolina
Ito Y, Okuda Shimazaki J, Tsugawa W, Loew N, Shitanda I, Lin C, et al
. Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase. Biosens Bioelectron. 2019;129:189-197 pubmed publisher
..02-0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe. ..
Lee I, Loew N, Tsugawa W, Ikebukuro K, Sode K. Development of a third-generation glucose sensor based on the open circuit potential for continuous glucose monitoring. Biosens Bioelectron. 2019;124-125:216-223 pubmed publisher
Hatada M, Tran T, Tsugawa W, Sode K, Mulchandani A. Affinity sensor for haemoglobin A1c based on single-walled carbon nanotube field-effect transistor and fructosyl amino acid binding protein. Biosens Bioelectron. 2019;129:254-259 pubmed publisher
..We propose that the modulation of the surface charges on the SWNTs caused by the conformational change in SocA upon ligand binding leads to the proportionate changes in the number of carriers in the SWNT channel. ..
Hatada M, Loew N, Inose Takahashi Y, Okuda Shimazaki J, Tsugawa W, Mulchandani A, et al
. Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference. Bioelectrochemistry. 2018;121:185-190 pubmed publisher
..5th generation principle can be developed for various target molecules. ..
Yamashita Y, Suzuki N, Hirose N, Kojima K, Tsugawa W, Sode K. Mutagenesis Study of the Cytochrome c Subunit Responsible for the Direct Electron Transfer-Type Catalytic Activity of FAD-Dependent Glucose Dehydrogenase. Int J Mol Sci. 2018;19: pubmed publisher
..However, if the enzyme complex is immobilized on the electrode and is used as electron acceptors, electrons are passed to the electrode from heme 2. ..
Miyazaki R, Yamazaki T, Yoshimatsu K, Kojima K, Asano R, Sode K, et al
. Elucidation of the intra- and inter-molecular electron transfer pathways of glucoside 3-dehydrogenase. Bioelectrochemistry. 2018;122:115-122 pubmed publisher
..Furthermore, we demonstrated that CYTc mediate the electron transfer from G3DH to electrode without the artificial electron mediator. ..
Okurita M, Suzuki N, Loew N, Yoshida H, Tsugawa W, Mori K, et al
. Engineered fungus derived FAD-dependent glucose dehydrogenase with acquired ability to utilize hexaammineruthenium(III) as an electron acceptor. Bioelectrochemistry. 2018;123:62-69 pubmed publisher
..Utilization of this new, improved fungal FADGDH should lead to the development of sensor strips for blood glucose monitoring with increased accuracy and less stringent packing requirements. ..
Ito K, Okuda Shimazaki J, Mori K, Kojima K, Tsugawa W, Ikebukuro K, et al
. Designer fungus FAD glucose dehydrogenase capable of direct electron transfer. Biosens Bioelectron. 2019;123:114-123 pubmed publisher
..These relevant and consistent findings provide us with a novel strategic approach for the improvement of the DET properties of designer FADGDH. (241 words). ..