M D Sobsey

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. pmc Virus transfer from personal protective equipment to healthcare employees' skin and clothing
    Lisa Casanova
    University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Emerg Infect Dis 14:1291-3. 2008
  2. doi request reprint Where future emerging pathogens will come from and what approaches can be used to find them, besides VFARs
    Mark D Sobsey
    Department of Environmental Sciences and Engineering, University of North Carolina, CB 7431, Rosenau Hall, Room 148, Chapel Hill, NC 27599 7431, USA
    J Water Health 7:S75-93. 2009
  3. ncbi request reprint Factors influencing faecal contamination in coastal marinas
    M D Sobsey
    University of North Carolina, Dept of Environmental Sciences and Engineering, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:199-204. 2003
  4. ncbi request reprint Chlorination and safe storage of household drinking water in developing countries to reduce waterborne disease
    M D Sobsey
    Dept of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:221-8. 2003
  5. ncbi request reprint Drinking water and health research: a look to the future in the United States and globally
    Mark D Sobsey
    University of North Carolina, CB 7431, McGavran Greenberg Hall, Room 4114a, Chapel Hill, NC 27599 7431, USA
    J Water Health 4:17-21. 2006
  6. pmc Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR
    K J Schwab
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7400, USA
    Appl Environ Microbiol 62:2086-94. 1996
  7. pmc Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR
    K J Schwab
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7400, USA
    Appl Environ Microbiol 61:531-7. 1995
  8. doi request reprint A simple and novel method for recovering adenovirus 41 in small volumes of source water
    J Wu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC, USA
    J Appl Microbiol 110:1332-40. 2011
  9. doi request reprint Reductions of E. coli, echovirus type 12 and bacteriophages in an intermittently operated household-scale slow sand filter
    M A Elliott
    University of North Carolina, CB 7431, Chapel Hill, NC 27599 7431, USA
    Water Res 42:2662-70. 2008
  10. doi request reprint Virus attenuation by microbial mechanisms during the idle time of a household slow sand filter
    M A Elliott
    The Water Institute at UNC, Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Campus Box 7431, Chapel Hill, NC 27599, USA
    Water Res 45:4092-102. 2011

Detail Information

Publications71

  1. pmc Virus transfer from personal protective equipment to healthcare employees' skin and clothing
    Lisa Casanova
    University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Emerg Infect Dis 14:1291-3. 2008
    ..Following this protocol often resulted in virus transfer to hands and clothing. An altered protocol or other measures are needed to prevent healthcare worker contamination...
  2. doi request reprint Where future emerging pathogens will come from and what approaches can be used to find them, besides VFARs
    Mark D Sobsey
    Department of Environmental Sciences and Engineering, University of North Carolina, CB 7431, Rosenau Hall, Room 148, Chapel Hill, NC 27599 7431, USA
    J Water Health 7:S75-93. 2009
    ..Understanding the microbial metagenome of the human body can also lead to a better understanding of how we define and characterize pathogens, commensals and opportunists...
  3. ncbi request reprint Factors influencing faecal contamination in coastal marinas
    M D Sobsey
    University of North Carolina, Dept of Environmental Sciences and Engineering, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:199-204. 2003
    ..Therefore, greater consideration of human health risks from enteric microbes in marina waters and shellfish is recommended...
  4. ncbi request reprint Chlorination and safe storage of household drinking water in developing countries to reduce waterborne disease
    M D Sobsey
    Dept of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:221-8. 2003
    ..Widespread use of this simple treatment and storage system for non-piped domestic water has the potential to dramatically reduce the global burden of waterborne diarrhoeal disease...
  5. ncbi request reprint Drinking water and health research: a look to the future in the United States and globally
    Mark D Sobsey
    University of North Carolina, CB 7431, McGavran Greenberg Hall, Room 4114a, Chapel Hill, NC 27599 7431, USA
    J Water Health 4:17-21. 2006
    ....
  6. pmc Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR
    K J Schwab
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7400, USA
    Appl Environ Microbiol 62:2086-94. 1996
    ..The results of enteric viruses were compared with those for standard bacterial and coliphage indicators of fecal contamination...
  7. pmc Concentration and purification of beef extract mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse transcription-PCR
    K J Schwab
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7400, USA
    Appl Environ Microbiol 61:531-7. 1995
    ..As little as 3 PFU of poliovirus type 1 in an initial 1 liter of mock eluate was detected by the RT-PCR...
  8. doi request reprint A simple and novel method for recovering adenovirus 41 in small volumes of source water
    J Wu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC, USA
    J Appl Microbiol 110:1332-40. 2011
    ..A new procedure was developed to recover adenovirus 41 in small volumes (1 l) of water samples based on adsorption, elution and evaporation...
  9. doi request reprint Reductions of E. coli, echovirus type 12 and bacteriophages in an intermittently operated household-scale slow sand filter
    M A Elliott
    University of North Carolina, CB 7431, Chapel Hill, NC 27599 7431, USA
    Water Res 42:2662-70. 2008
    ..3 log10 (95%) with mean reductions of only 0.5 log10 (70%). These data indicate that virus reduction by BSF may differ substantially depending upon the specific viral agent...
  10. doi request reprint Virus attenuation by microbial mechanisms during the idle time of a household slow sand filter
    M A Elliott
    The Water Institute at UNC, Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Campus Box 7431, Chapel Hill, NC 27599, USA
    Water Res 45:4092-102. 2011
    ..Implications of these findings for BSF design and operation and their relevance to other biological filtration technologies are discussed...
  11. pmc A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization
    L A Jaykus
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill 27599 7400, USA
    Appl Environ Microbiol 62:2074-80. 1996
    ..The procedure developed in this study is rapid, sensitive, and effective for the direct detection of enteric viruses in oysters by RT-PCR...
  12. ncbi request reprint Fecal contamination of agricultural soils before and after hurricane-associated flooding in North Carolina
    Michael J Casteel
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
    J Environ Sci Health A Tox Hazard Subst Environ Eng 41:173-84. 2006
    ....
  13. pmc Evaluation of F+ RNA and DNA coliphages as source-specific indicators of fecal contamination in surface waters
    Dana Cole
    Department of Environmental Sciences and Engineering, The University of North Carolina School of Public Health, Chapel Hill, North Carolina 27599, USA
    Appl Environ Microbiol 69:6507-14. 2003
    ..Monitoring of F+ coliphage groups can indicate the presence and major sources of microbial inputs to surface waters, but environmental effects on the relative occurrence of different groups need to be considered...
  14. ncbi request reprint Development and evaluation of methods to detect coliphages in large volumes of water
    M D Sobsey
    Dept of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Water Sci Technol 50:211-7. 2004
    ..Overall, the enrichment method was preferred because of its ability to easily and rapidly detect low levels of coliphages in large sample volumes by either presence-absence or most probable number quantification...
  15. ncbi request reprint Comparative survival of enteric viruses and bacteria in Atlantic Ocean seawater
    D A Wait
    Dept of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599-7400, USA
    Water Sci Technol 43:139-42. 2001
    ..The lower survival of E. coli compared to the bacterial and viral pathogens under laboratory conditions raises concerns because it is a key microbial indicator of faecal contamination...
  16. ncbi request reprint Removal of Salmonella and microbial indicators in constructed wetlands treating swine wastewater
    V R Hill
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina at Chapel Hill, 27599, USA
    Water Sci Technol 44:215-22. 2001
    ..These results show that SF constructed wetlands can be effective for reducing enteric pathogens in swine wastewater and that greater removals can be achieved using SSF designs and lower TKN loading rates...
  17. pmc Male-specific coliphages as indicators of thermal inactivation of pathogens in biosolids
    Sharon P Nappier
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Appl Environ Microbiol 72:2471-5. 2006
    ..Further studies should focus on the group III F+ RNA coliphages as potential indicators of reductions of heat-resistant pathogens in thermal processes for sludge treatment...
  18. pmc Recovery and sequence analysis of hepatitis a virus from springwater implicated in an outbreak of acute viral hepatitis
    Lindsay A Tallon
    North Carolina Center for Public Health Preparedness, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Appl Environ Microbiol 74:6158-60. 2008
    ..Detection of HAV and fecal indicators in the water provided useful and timely information to assist with public health prevention and control measures...
  19. pmc Concentration and detection of cryptosporidium oocysts in surface water samples by method 1622 using ultrafiltration and capsule filtration
    O D Simmons
    School of Public Health, University of North Carolina Chapel Hill, Chapel Hill, North Carolina 27599 7400, USA
    Appl Environ Microbiol 67:1123-7. 2001
    ..parvum oocysts from seeded surface waters with significantly greater efficiency and reliability than the filter suggested for use in the version of method 1622 tested...
  20. ncbi request reprint Characterisation of the biosand filter for E. coli reductions from household drinking water under controlled laboratory and field use conditions
    C E Stauber
    University of North Carolina Chapel Hill, Dept of Environmental Sciences and Engineering, 27599 Chapel Hill, NC, USA
    Water Sci Technol 54:1-7. 2006
    ..Further study is needed to determine the factors contributing to microbial reductions in BSFs and why reductions are lower than those of conventional SSFs...
  21. pmc Bacterial indicators of risk of diarrhoeal disease from drinking-water in the Philippines
    C L Moe
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill
    Bull World Health Organ 69:305-17. 1991
    ....
  22. doi request reprint Comparing the partitioning behavior of Giardia and Cryptosporidium with that of indicator organisms in stormwater runoff
    Adrienne R Cizek
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Water Res 42:4421-38. 2008
    ..The total loadings of both indicators and pathogens were also estimated over the course of individual storms...
  23. pmc Evaluation of liquid- and fog-based application of Sterilox hypochlorous acid solution for surface inactivation of human norovirus
    Geun woo Park
    Department of Environmental Sciences and Engineering, Univerisity of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Appl Environ Microbiol 73:4463-8. 2007
    ..9% (3 log10), regardless of carrier location and orientation. We conclude that HOCl solution as a liquid or fog is likely to be effective in disinfecting common settings to reduce NV exposures and thereby control virus spread via fomites...
  24. ncbi request reprint Evaluation of RT-PCR and reverse line blot hybridization for detection and genotyping F+ RNA coliphages from estuarine waters and molluscan shellfish
    D C Love
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    J Appl Microbiol 104:1203-12. 2008
    ....
  25. doi request reprint Inactivation of murine norovirus, feline calicivirus and echovirus 12 as surrogates for human norovirus (NoV) and coliphage (F+) MS2 by ultraviolet light (254 nm) and the effect of cell association on UV inactivation
    G W Park
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC, USA
    Lett Appl Microbiol 52:162-7. 2011
    ..To determine inactivation profiles of three human norovirus (NoV) surrogate viruses and coliphage MS2 by ultraviolet (UV) irradiation and the protective effect of cell association on UV inactivation...
  26. ncbi request reprint Inactivation of enteric microbes in water by electro-chemical oxidant from brine (NaCl) and free chlorine
    L V Venczel
    Dept of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Water Sci Technol 50:141-6. 2004
    ..However, the effectiveness of such oxidants for inactivating C. parvum oocysts was variable and sometimes ineffective...
  27. ncbi request reprint Evaluation of positively charged alumina nanofibre cartridge filters for the primary concentration of noroviruses, adenoviruses and male-specific coliphages from seawater
    C D Gibbons
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    J Appl Microbiol 109:635-41. 2010
    ..To evaluate the electropositive, alumina nanofibre (NanoCeram) cartridge filter as a primary concentration method for recovering adenovirus, norovirus and male-specific coliphages from natural seawater...
  28. pmc Low-pressure UV inactivation and DNA repair potential of Cryptosporidium parvum oocysts
    G A Shin
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599 7400, USA
    Appl Environ Microbiol 67:3029-32. 2001
    ..These results indicate that C. parvum oocysts are very sensitive to inactivation by low doses of monochromatic LP UV radiation and that there is no phenotypic evidence of either light or dark repair of UV-induced DNA damage...
  29. ncbi request reprint Comparative reduction of Norwalk virus, poliovirus type 1, F+ RNA coliphage MS2 and Escherichia coli in miniature soil columns
    J S Meschke
    Dept of Environmental Sciences and Engineering, School of Public Health, University of North Carolina Chapel Hill, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:85-90. 2003
    ..Elution of viruses, in particular PV1, from the columns was gradual. After cessation of microbe dosing, E. coli was less detectable than viruses in column effluents and, therefore, unreliable as a virus indicator...
  30. pmc Efficacy of hospital germicides against adenovirus 8, a common cause of epidemic keratoconjunctivitis in health care facilities
    William A Rutala
    Department of Hospital Epidemiology, University of North Carolina Health Care System, Chapel Hill, NC 27514, USA
    Antimicrob Agents Chemother 50:1419-24. 2006
    ....
  31. ncbi request reprint Intra-storm variability in microbial partitioning and microbial loading rates
    Leigh Anne H Krometis
    Department of Environmental Sciences and Engineering, School of Public Health, Rosenau Hall CB 7431, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Water Res 41:506-16. 2007
    ..g., detention basins). Estimates of cumulative storm-induced microbial loading suggested that one day's worth of storm loading can be the equivalent of months, or even years, of dry-weather loading...
  32. ncbi request reprint Inactivation of Escherichia coli O157:H7 during thermophilic anaerobic digestion of manure from dairy cattle
    Michael D Aitken
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Water Res 41:1659-66. 2007
    ..Therefore, more specific methods should be used to evaluate the extent of thermal inactivation of both pathogenic and non-pathogenic E. coli in manure treatment systems...
  33. pmc Simple and rapid F+ coliphage culture, latex agglutination, and typing assay to detect and source track fecal contamination
    David C Love
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599, USA
    Appl Environ Microbiol 73:4110-8. 2007
    ....
  34. ncbi request reprint Effect of specialized bathing systems on resident cleanliness and water quality in nursing homes: a randomized controlled trial
    Philip D Sloane
    Cecil G Sheps Center for Health Services Research, The University of North Carolina at Chapel Hill, 725 Airport Road, Chapel Hill NC 27599, USA
    J Water Health 5:283-94. 2007
    ..In addition, staff reported that the ultrasound tub using enhanced skin cleansers made bathing residents easier and faster than the same tub using standard cleansers...
  35. pmc Effects of air temperature and relative humidity on coronavirus survival on surfaces
    Lisa M Casanova
    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA
    Appl Environ Microbiol 76:2712-7. 2010
    ..TGEV and MHV could serve as conservative surrogates for modeling exposure, the risk of transmission, and control measures for pathogenic enveloped viruses, such as SARS-CoV and influenza virus, on health care surfaces...
  36. ncbi request reprint Point of use household drinking water filtration: A practical, effective solution for providing sustained access to safe drinking water in the developing world
    Mark D Sobsey
    Department of Environmental Sciences and Engineering, CB 7431, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Environ Sci Technol 42:4261-7. 2008
    ....
  37. doi request reprint Survival of surrogate coronaviruses in water
    Lisa Casanova
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    Water Res 43:1893-8. 2009
    ..Coronaviruses can remain infectious for long periods in water and pasteurized settled sewage, suggesting contaminated water is a potential vehicle for human exposure if aerosols are generated...
  38. ncbi request reprint Laboratory evaluation of thermophilic-anaerobic digestion to produce Class A biosolids. 2. Inactivation of pathogens and indicator organisms in a continuous-flow reactor followed by batch treatment
    Michael D Aitken
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7431, USA
    Water Environ Res 77:3028-36. 2005
    ..Over the range of temperatures evaluated, the maximum time required to meet the Class A criteria by batch treatment of the continuous-reactor effluent was 1 hour for Ascaris suum and Salmonella spp. and 2 hours for fecal coliforms...
  39. ncbi request reprint Inactivation of Ascaris suum and poliovirus in biosolids under thermophilic anaerobic digestion conditions
    Michael D Aitken
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Environ Sci Technol 39:5804-9. 2005
    ..Such a grossly conservative approach can hinder full-scale implementation of thermophilic anaerobic digestion...
  40. ncbi request reprint Isolation and characterization of circulating type 1 vaccine-derived poliovirus from sewage and stream waters in Hispaniola
    Jan Vinje
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    J Infect Dis 189:1168-75. 2004
    ..Our findings demonstrate a useful role for environmental surveillance of neurovirulent polioviruses in the overall polio eradication program...
  41. ncbi request reprint Development and application of a capsid VP1 (region D) based reverse transcription PCR assay for genotyping of genogroup I and II noroviruses
    Jan Vinje
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    J Virol Methods 116:109-17. 2004
    ..In summary, we successfully developed and evaluated a broadly reactive RT-PCR assay for reliable genotyping of GI and GII noroviruses...
  42. pmc Molecular detection and genotyping of male-specific coliphages by reverse transcription-PCR and reverse line blot hybridization
    Jan Vinje
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    Appl Environ Microbiol 70:5996-6004. 2004
    ..In summary, we developed a novel method for standardized genotyping of F+ coliphages as a useful tool for large-scale MST studies...
  43. ncbi request reprint Comparative efficacy of hand hygiene agents in the reduction of bacteria and viruses
    Emily E Sickbert-Bennett
    Department of Hospital Epidemiology, University of North Carolina Health Care System, North Carolina, USA
    Am J Infect Control 33:67-77. 2005
    ..Health care-associated infections most commonly result from person-to-person transmission via the hands of health care workers...
  44. pmc Detection of infectious adenovirus in cell culture by mRNA reverse transcription-PCR
    Gwangpyo Ko
    The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA
    Appl Environ Microbiol 69:7377-84. 2003
    ..These results suggest that mRNA detection by RT-PCR assay in inoculated cell cultures is a very sensitive, specific, and rapid method by which to detect infectious Ads in water and other environmental samples...
  45. ncbi request reprint Microbial partitioning to settleable particles in stormwater
    Gregory W Characklis
    Department of Environmental Sciences and Engineering, School of Public Health, Rosenau Hall CB7431, University of North Carolina, Chapel Hill, NC 27599 7431, USA
    Water Res 39:1773-82. 2005
    ..These estimates should be valuable in surface water quality modeling efforts, many of which currently assume that all microbes exist as free (unattached) organisms...
  46. pmc Reduction of Norwalk virus, poliovirus 1, and bacteriophage MS2 by ozone disinfection of water
    Gwy Am Shin
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7400, USA
    Appl Environ Microbiol 69:3975-8. 2003
    ....
  47. ncbi request reprint Use of viral pathogens and indicators to differentiate between human and non-human fecal contamination in a microbial source tracking comparison study
    Rachel T Noble
    UNC Chapel Hill Institute of Marine Sciences, Morehead City, NC 28557, USA
    J Water Health 1:195-207. 2003
    ..Overall, virus-based source tracking methods are an important approach to include in the microbial source tracking 'toolbox'...
  48. doi request reprint Methods for the recovery of a model virus from healthcare personal protective equipment
    L Casanova
    Department of Environmental Sciences and Engineering, University of North Carolina Chapel Hill, 27599, USA
    J Appl Microbiol 106:1244-51. 2009
    ..To develop methods for recovering a model virus (bacteriophage MS2) from healthcare personal protective equipment (PPE)...
  49. ncbi request reprint Detection and occurrence of antimicrobially resistant E. coli in groundwater on or near swine farms in eastern North Carolina
    M E Anderson
    University of North Carolina at Chapel Hill, School of Public Health, Rosenau Hall CB 7400, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 54:211-8. 2006
    ..The results of this study demonstrate that antibiotic-resistant E. coli strains are present in groundwaters of swine farms with a typical lagoon and land application system for waste management...
  50. ncbi request reprint A chemiluminescent immunofocus assay (CIFA) for non-microscopic enumeration of Cryptosporidium parvum infectivity in cell culture
    O D Simmons
    University of North Carolina Chapel Hill, School of Public Health, Rosenau Hall, CB 7400, Chapel Hill, NC 27599 7400, USA
    Water Sci Technol 47:137-42. 2003
    ..These objects are directly countable with the unaided eye and their identity can be further confirmed or verified by microscopic examination...
  51. ncbi request reprint Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
    E Suñén
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599 7400, USA
    J Virol Methods 77:179-87. 1999
    ..Both methods gave similar efficiency for virus detection in samples seeded with low virus levels. The procedure developed in this study is effective for enteric viruses detection in hardshell clams by RT-PCR...
  52. ncbi request reprint A randomized controlled trial of the concrete biosand filter and its impact on diarrheal disease in Bonao, Dominican Republic
    Christine E Stauber
    Department of Environmental Science and Engineering, School of Public Health, The University of North Carolina, Chapel Hill, North Carolina 27599 7431, USA
    Am J Trop Med Hyg 80:286-93. 2009
    ..001). Based on random intercepts logistic regression, BSF households had 0.53 times the odds of diarrheal disease as control households, indicating a significant protective effect of the BSF against waterborne diarrheal disease...
  53. doi request reprint Methods for recovery of hepatitis A virus (HAV) and other viruses from processed foods and detection of HAV by nested RT-PCR and TaqMan RT-PCR
    David C Love
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States
    Int J Food Microbiol 126:221-6. 2008
    ....
  54. doi request reprint Chlorine disinfection of produce to inactivate hepatitis A virus and coliphage MS2
    Michael J Casteel
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Int J Food Microbiol 125:267-73. 2008
    ....
  55. ncbi request reprint Development of a L-rhamnose and D-arabitol supplemented MacConkey agar to identify pathogenic Yersinia enterocolitica among environmental Yersinias in swine production wastes
    M W Shehee
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill, NC 27599, USA
    J Microbiol Methods 57:289-92. 2004
    ..This medium has a sensitivity of 100% and a specificity of 97.4%...
  56. ncbi request reprint Isolation, detection and characterization of swine hepatitis E virus from herds in Costa Rica
    Julie A Kase
    Department of Environmental Sciences and Engineering, The School of Public Health, University of North Carolina, Chapel Hill, North Carolina, USA
    Int J Environ Health Res 18:165-76. 2008
    ..By comparison of swine and human HEV strains representing all four genotypes and phylogenetic analysis, our isolates closely resembled the US swine and human and other Genotype III strains, with 85-93% nucleic acid identity...
  57. doi request reprint Microbiological effectiveness of locally produced ceramic filters for drinking water treatment in Cambodia
    Joe Brown
    Department of Environmental Sciences and Engineering, University of North Carolina School of Public Health, CB 7431 Rosenau Hall, Chapel Hill, NC 27599 7431, USA
    J Water Health 8:1-10. 2010
    ..Increased effectiveness was not observed in filters with an AgNO3 amendment. At under US$10 per filter, locally produced ceramic filters may be a promising option for drinking water treatment and safe storage at the household level...
  58. ncbi request reprint Water quality and health in the new millennium: the role of the World Health Organization Guidelines for Drinking-Water Quality
    Mark D Sobsey
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina, Chapel Hill, USA
    Forum Nutr 56:396-405. 2003
    ..quot;..
  59. ncbi request reprint Enteric bacteriophages as potential fecal indicators in ground beef and poultry meat
    F C Hsu
    Department of Environmental Sciences and Engineering, University of North Carolina, Chapel Hill 27599, USA
    J Food Prot 65:93-9. 2002
    ..The bacteriophage concentration method developed provides a simple, rapid, and practical tool for the evaluation of fecal contamination levels in ground beef and processed chicken meat...
  60. pmc Development and evaluation of a broadly reactive TaqMan assay for rapid detection of hepatitis A virus
    N Jothikumar
    Department of Environmental Sciences and Engineering, University of North Carolina at Chapel Hill, 27599 7431, USA
    Appl Environ Microbiol 71:3359-63. 2005
    ..The assay detected 0.5 infectious units of HAV and 40 copies of a synthetic transcript and provides an important screening tool for rapid quantitative HAV detection in clinical or environmental samples...
  61. ncbi request reprint Photoinactivation of hepatitis A virus by synthetic porphyrins
    Michael J Casteel
    Department of Environmental Sciences and Engineering, School of Public Health, University of North Carolina Chapel Hill, Chapel Hill, NC, USA
    Photochem Photobiol 80:294-300. 2004
    ..Disinfection by photoactivated synthetic porphyrins therefore can offer an effective and relatively safe approach to removal of nonenveloped viruses from aqueous media...
  62. ncbi request reprint UV inactivation of adenovirus type 41 measured by cell culture mRNA RT-PCR
    Gwangpyo Ko
    University of Texas School of Public Health, Houston, TX 77225, USA
    Water Res 39:3643-9. 2005
    ..Results were more similar to those found for Ad 40 using CPE as a measure of infectivity in another previous study...
  63. ncbi request reprint Presence, infectivity, and stability of enteric viruses in seawater: relationship to marine water quality in the Florida Keys
    Jennifer Jarrell Wetz
    College of Oceanic and Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, USA
    Mar Pollut Bull 48:698-704. 2004
    ....
  64. ncbi request reprint Reduction of enteric microbes in flushed swine wastewater treated by a biological aerated filter and UV irradiation
    Vincent R Hill
    School of Public Health, University of North Carolina at Chapel Hill, USA
    Water Environ Res 74:91-9. 2002
    ....
  65. ncbi request reprint Local drinking water filters reduce diarrheal disease in Cambodia: a randomized, controlled trial of the ceramic water purifier
    Joe Brown
    University of Alabama, Department of Biological Sciences, Tuscaloosa, AL 35487 0229, USA
    Am J Trop Med Hyg 79:394-400. 2008
    ..51 (95% confidence interval [CI]: 0.41-0.63); CWP-Fe: 0.58 (95% CI: 0.47-0.71), an effect that was observed in all age groups and both sexes after controlling for clustering within households and within individuals over time...
  66. pmc Sequence variation among group III F-specific RNA coliphages from water samples and swine lagoons
    Jill R Stewart
    National Oceanic and Atmospheric Administration, 219 Ft Johnson Rd, Charleston, SC 29412, USA
    Appl Environ Microbiol 72:1226-30. 2006
    ..A similar use of phage genomic sequence information to track fecal pollution promises more reliable results than phage typing by nucleic acid hybridization and may hold more potential for field applications...
  67. ncbi request reprint A comparison of the survival of F+RNA and F+DNA coliphages in lake water microcosms
    Sharon C Long
    Civil and Environmental Engineering, University of Massachusetts, Amherst, MA 01003, USA
    J Water Health 2:15-22. 2004
    ..These results suggest that differences in F+ phage survival may influence their prevalence in environmental waters and the ability to attribute their prevalence to specific human and animal sources of faecal contamination...
  68. ncbi request reprint Rapid detection of infectious adenoviruses by mRNA real-time RT-PCR
    Gwangpyo Ko
    University of Texas Health Science Center, School of Public Health, 1200 Herman Pressler Dr RAS W 634, Houston, TX 77225, USA
    J Virol Methods 127:148-53. 2005
    ..These results suggest that mRNA detection by real-time RT-PCR is a sensitive and specific method to detect low levels of infectious adenoviruses in water and other environmental media within 1-3 days...
  69. pmc Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41
    Narayanan Jothikumar
    Centers for Disease Control and Prevention, 4770 Buford Highway, Mailstop F 36, Atlanta, Georgia 30341, USA
    Appl Environ Microbiol 71:3131-6. 2005
    ..Application of these assays for detection of adenoviruses and type-specific identification of AdV40 and AdV41 will be useful for identifying these viruses in environmental and clinical samples...
  70. ncbi request reprint UV disinfection of Giardia lamblia cysts in water
    Karl G Linden
    Department of Civil and Environmental Engineering, Duke University, Durham, North Carolina 27708, USA
    Environ Sci Technol 36:2519-22. 2002
    ..We conclude that UV disinfection at practical doses achieves appreciable (much greater than 4 log) inactivation of G. lamblia cysts in water with no evidence of DNA repair leading to infectivity reactivation...
  71. pmc Effects of seeding procedures and water quality on recovery of Cryptosporidium oocysts from stream water by using U.S. Environmental Protection Agency Method 1623
    Donna S Francy
    U S Geological Survey, Water Resources Discipline, 6480 Doubletree Ave, Columbus, OH 43229, USA
    Appl Environ Microbiol 70:4118-28. 2004
    ..802) and were not significantly different. Recoveries by using modified oocysts, therefore, were comparable to recoveries by using conventional seeding procedures...