Research Topics
Genomes and Genes | MALCOLM M SMITHSummaryAffiliation: University of Virginia Country: USA Publications
Research Grants
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Detail Information
Publications
Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexesM S Santisteban
Department of Microbiology and Cancer Center, University of Virginia, Charlottesville 22908, USA
Cell 103:411-22. 2000..These results describe a novel pathway for regulating transcription using variant histones to modulate chromatin structure...
A unified phylogeny-based nomenclature for histone variantsPaul B Talbert
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA, 98109, USA
Epigenetics Chromatin 5:7. 2012..For clarity and searchability, we encourage the use of descriptors that are separate from the phylogeny-based variant name to indicate developmental and other properties of variants that may be independent of structure...- Histone variant H2A.Z and RNA polymerase II transcription elongationMaria Soledad Santisteban
Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
Mol Cell Biol 31:1848-60. 2011.... - Genetic dissection of histone functionM M Smith
Department of Microbiology, University of Virginia, Charlottesville, USA
Methods 15:269-81. 1998..The conservation of histone and nucleosome structure throughout evolution means that the principles discovered through genetic studies in yeast will be broadly applicable to the chromatin of more complex eukaryotes... - Centromeres and variant histones: what, where, when and why?M Mitchell Smith
Department of Microbiology and University of Virginia Cancer Center, Jordan Building, Room 7223, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA
Curr Opin Cell Biol 14:279-85. 2002..Finally, a new report provides a glimpse into the potential regulation of CENP-A through specific post-translational phosphorylation, suggesting a broad level of control through histone tail modifications... - Histone octamer function in vivo: mutations in the dimer-tetramer interfaces disrupt both gene activation and repressionM S Santisteban
Department of Microbiology and University of Virginia Cancer Center, School of Medicine, Charlottesville 22908, USA
EMBO J 16:2493-506. 1997..These results demonstrate the critical role of histone dimer-tetramer interactions in vivo, and define their essential role in the expression of genes regulating G1 cell cycle progression... - Histone-histone interactions and centromere functionL Glowczewski
Department of Microbiology and Cancer Center, University of Virginia, Charlottesville, Virginia 22908, USA
Mol Cell Biol 20:5700-11. 2000..However, the relative resistance of the Cse4p-H4 pathway to H3 interference argues that centromere chromatin assembly must be highly regulated... - Cyclic AMP signaling is required for function of the N-terminal and CR1 domains of adenovirus E1A in Saccharomyces cerevisiaeM E Miller
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908, USA
Oncogene 11:1623-30. 1995..These results suggest that a genetic analysis of the yeast model system will provide relevant new insights into mechanisms of gene regulation by E1A proteins... - Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodesJ Y Hsu
Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville 22908, USA
Cell 102:279-91. 2000..Our findings reveal an enzyme system that regulates chromosome dynamics and controls histone phosphorylation that is conserved among diverse eukaryotes... - A novel histone H4 mutant defective in nuclear division and mitotic chromosome transmissionM M Smith
Department of Microbiology, University of Virginia Cancer Center, Charlottesville, 22908, USA
Mol Cell Biol 16:1017-26. 1996..These genetic, biochemical, and phenotypic results indicate that this novel histone H4 mutant defines one or more chromatin-dependent steps in chromosome segregation... - Influence of the adenovirus 5 E1A oncogene on chromatin remodellingJ S Mymryk
Department of Microbiology and University of Virginia Cancer Center, School of Medicine, University of Virginia, Charlottesville 22908, USA
Biochem Cell Biol 75:95-102. 1997..This review focuses on the unexpected effects of E1A on cellular processes that remodel chromatin in relation to its transcriptional and transforming activities... - Histone variants and nucleosome deposition pathwaysM Mitchell Smith
Department of Microbiology and University of Virginia Cancer Center, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA 22901, USA
Mol Cell 9:1158-60. 2002..These results have important implications for chromatin remodeling and epigenetic imprinting... - Nonhistone Scm3 and histones CenH3-H4 assemble the core of centromere-specific nucleosomesGaku Mizuguchi
Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Building 37, Room 6068, Bethesda, MD 20892 4255, USA
Cell 129:1153-64. 2007..Our findings indicate that nonhistone Scm3 serves to assemble and maintain Cse4-H4 at centromeres and may replace histone H2A-H2B dimers in a centromere-specific nucleosome core... - Adenovirus E1A requires the yeast SAGA histone acetyltransferase complex and associates with SAGA components Gcn5 and Tra1Caroline A Kulesza
Department of Microbiology and Cancer Center, University of Virginia School of Medicine, Charlottesville, Virginia, VA 22908, USA
Oncogene 21:1411-22. 2002..These results indicate a strong requirement for intact SAGA complex for E1A to function in yeast, and suggest a role for SAGA-like complexes in mammalian cell transformation... - Catalytic-site mutations in the MYST family histone Acetyltransferase Esa1Peter V Decker
Department of Microbiology, University of Virginia Cancer Center, University of Virginia Health System, Charlottesville, Virginia 22908, USA
Genetics 178:1209-20. 2008..We propose that the essential function of Esa1 may be to bind acetyl-CoA or lysine substrates and positively regulate the activities of NuA4 and Piccolo NuA4... - Hbo1 Links p53-dependent stress signaling to DNA replication licensingMasayoshi Iizuka
Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
Mol Cell Biol 28:140-53. 2008..These results reveal a direct regulatory connection between p53-responsive stress signaling and Hbo1-dependent chromatin pathways... - Regulation of replication licensing by acetyltransferase Hbo1Masayoshi Iizuka
Department of Microbiology, University of Virginia Health System, P O Box 800734, Charlottesville, VA 22908 0734, USA
Mol Cell Biol 26:1098-108. 2006..The binding of Mcm2-7 to chromatin in XHbo1-depleted extracts could be restored by the addition of recombinant Cdt1... - Sterile 20 kinase phosphorylates histone H2B at serine 10 during hydrogen peroxide-induced apoptosis in S. cerevisiaeSung Hee Ahn
Laboratory of Chromatin Biology, The Rockefeller University, Box 78, New York, NY 10021, USA
Cell 120:25-36. 2005.... - Yaf9, a novel NuA4 histone acetyltransferase subunit, is required for the cellular response to spindle stress in yeastIvan Le Masson
Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, France
Mol Cell Biol 23:6086-102. 2003..These results strongly suggest that acetylation of histone H4 by NuA4 is required for the cellular resistance to spindle stress... - Functional consequences of histone modificationsMasayoshi Iizuka
Department of Microbiology, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
Curr Opin Genet Dev 13:154-60. 2003..Finally, exciting research has identified new modifications, complex modification cascades, and functional links to DNA methylation and RNA interference pathways... - Global and specific transcriptional repression by the histone H3 amino terminus in yeastNevin Sabet
Laboratory of Developmental Genetics, Wadsworth Center, New York State Department of Health, Albany, NY 12201 2002, USA
Proc Natl Acad Sci U S A 100:4084-9. 2003..We also present results of a microarray experiment showing that the H3 amino terminus has a substantial repressive effect on a genome-wide scale... - Acetylation of histone H4 by Esa1 is required for DNA double-strand break repairAlexander W Bird
Department of Microbiology, University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, Virginia 22908, USA
Nature 419:411-5. 2002..Together, our data show that histone tail acetylation is required directly for DNA repair and suggest that a related human HAT complex may function similarly...
Research Grants
- THE ROLE OF HISTONE H4 IN GENOME STABILITYM Smith; Fiscal Year: 2003..Our observation that reversible histone H4 acetylation is required for DNA repair represents a previously unrecognized role for this important chromatin modification. ..
- THE ROLE OF HISTONE H4 IN GENOME STABILITYM Smith; Fiscal Year: 2007..abstract_text> ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 2007..To understand the rules and mechanisms for these assembly patterns, we will engineer mutations in specific cis- and trans-acting factors to test alternative models of Htz1 recruitment. ..
- Epigenetic Regulation of Gene Expression During Early Mouse EmbryogenesisM Smith; Fiscal Year: 2007..The results of these studies will have a significant impact on how we think about, and manage, a wide range of human health issues including assisted reproductive techniques, somatic nuclear transfer, and stem cell therapy. ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1999..We propose that histone H4 and CENP-A form a specialized nucleosome at the centromere and interact with other kinetochore proteins. This model will be tested both genetically and biochemically. ..
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1991..Both the transcription of these related genes and the chromatin structure of the mating type genes will be assayed...
- HISTONE GENE EXPRESSION IN YEASTM Smith; Fiscal Year: 1993..A mutational analysis of the UAS binding protein will be carried out to determine its molecular function, and define the signal transduction pathway for cell cycle position information...
- The Role of MYST Histone Acetyltransferase in Genome StabilityM Mitchell Smith; Fiscal Year: 2010..Little is known about their range of functions and target pathways. The research proposed in this application is designed to uncover new principles in how these enzymes work, what they do, and how they do it. ..