Harold C Smith

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. doi request reprint Functions and regulation of the APOBEC family of proteins
    Harold C Smith
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
    Semin Cell Dev Biol 23:258-68. 2012
  2. pmc APOBEC3G: a double agent in defense
    Harold C Smith
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
    Trends Biochem Sci 36:239-44. 2011
  3. pmc APOBEC3G subunits self-associate via the C-terminal deaminase domain
    Ryan P Bennett
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 283:33329-36. 2008
  4. pmc Functional characterization of APOBEC-1 complementation factor phosphorylation sites
    David M Lehmann
    Environmental Health Sciences Center, Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
    Biochim Biophys Acta 1773:408-18. 2007
  5. pmc Metabolic regulation of APOBEC-1 complementation factor trafficking in mouse models of obesity and its positive correlation with the expression of ApoB protein in hepatocytes
    Chad A Galloway
    University of Rochester, Department of Biochemistry and Biophysics, 601 Elmwood Ave Rochester, NY 14642, USA
    Biochim Biophys Acta 1802:976-85. 2010
  6. doi request reprint Nuclear Exclusion of the HIV-1 host defense factor APOBEC3G requires a novel cytoplasmic retention signal and is not dependent on RNA binding
    Ryan P Bennett
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 283:7320-7. 2008
  7. ncbi request reprint Structural phylogenetic analysis of activation-induced deaminase function
    H Travis Ichikawa
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 177:355-61. 2006
  8. pmc APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic
    Ryan P Bennett
    Department of Biochemistry, Box 712, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 350:214-9. 2006
  9. ncbi request reprint The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors
    Mark P Sowden
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Cell Sci 115:1027-39. 2002
  10. pmc Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factor
    David M Lehmann
    Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
    Nucleic Acids Res 34:3299-308. 2006

Collaborators

  • Joseph E Wedekind
  • David M Lehmann
  • Benjamin L Miller
  • Yan Yang
  • Adrian R Krainer
  • Jacques Robert
  • James H Miller
  • Ryan P Bennett
  • Mark P Sowden
  • Chad A Galloway
  • William M McDougall
  • Jason D Salter
  • Geoffrey S C Dance
  • Leslie O Ofori
  • H Travis Ichikawa
  • Andrew T Torelli
  • Kefang Xie
  • Nathaniel W Brown
  • Thomas A Hilimire
  • Chinelo Okany
  • Robert A Mooney
  • John Ashton
  • Janet D Sparks
  • Jolanta Krucinska
  • Jay Raina
  • Vladimir Presnyak
  • Xiang Liu
  • Elie Diner
  • Shauna H Marr
  • Joshua A Lees
  • Jurgen Bachl
  • Pinwei Huang
  • Andrea Bottaro
  • Charles O Smith
  • Xiaoyan Lin
  • Karen L de Mesy Jensen
  • Nazzareno Ballatori
  • Ellen Cooper
  • Luca Cartegni
  • Lakesha Hamilton Reed

Detail Information

Publications26

  1. doi request reprint Functions and regulation of the APOBEC family of proteins
    Harold C Smith
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
    Semin Cell Dev Biol 23:258-68. 2012
    ....
  2. pmc APOBEC3G: a double agent in defense
    Harold C Smith
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
    Trends Biochem Sci 36:239-44. 2011
    ..Future research should focus on understanding how cellular and viral regulatory mechanisms enable the antiviral function of A3G, and on the development of novel research reagents to explore these pathways...
  3. pmc APOBEC3G subunits self-associate via the C-terminal deaminase domain
    Ryan P Bennett
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 283:33329-36. 2008
    ..These findings corroborate the small angle x-ray scattering structural model and are instructive for development of high throughput screens that target specific domains and their functions to identify HIV/AIDS therapeutics...
  4. pmc Functional characterization of APOBEC-1 complementation factor phosphorylation sites
    David M Lehmann
    Environmental Health Sciences Center, Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
    Biochim Biophys Acta 1773:408-18. 2007
    ..These data suggest that phosphorylation of ACF by PKC may be a key regulatory mechanism of apoB mRNA editing in rat hepatocytes...
  5. pmc Metabolic regulation of APOBEC-1 complementation factor trafficking in mouse models of obesity and its positive correlation with the expression of ApoB protein in hepatocytes
    Chad A Galloway
    University of Rochester, Department of Biochemistry and Biophysics, 601 Elmwood Ave Rochester, NY 14642, USA
    Biochim Biophys Acta 1802:976-85. 2010
    ....
  6. doi request reprint Nuclear Exclusion of the HIV-1 host defense factor APOBEC3G requires a novel cytoplasmic retention signal and is not dependent on RNA binding
    Ryan P Bennett
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 283:7320-7. 2008
    ..The CRS lies in a region involved in both Gag and Vif interactions; therefore, identification of this motif has important implications for the design of therapeutics that target HIV-1 while maintaining antiviral and cellular functions...
  7. ncbi request reprint Structural phylogenetic analysis of activation-induced deaminase function
    H Travis Ichikawa
    Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Immunol 177:355-61. 2006
    ....
  8. pmc APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic
    Ryan P Bennett
    Department of Biochemistry, Box 712, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 350:214-9. 2006
    ..We demonstrate that hA3G is not a nucleo-cytoplasmic shuttling protein like APOBEC-1 and AID, but is strongly retained in the cytoplasm through a mechanism that involves both the N and C-terminal regions of the protein...
  9. ncbi request reprint The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factors
    Mark P Sowden
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Cell Sci 115:1027-39. 2002
    ..It is proposed that the heterogeneity in size of complexes containing editing factors is functionally significant and reflects functionally engaged editosomes in the nucleus and an inactive cytoplasmic pool of factors...
  10. pmc Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factor
    David M Lehmann
    Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
    Nucleic Acids Res 34:3299-308. 2006
    ....
  11. ncbi request reprint Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family business
    Joseph E Wedekind
    Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14623, USA
    Trends Genet 19:207-16. 2003
    ..In light of the hypothesis that these proteins might represent novel mRNA editing systems that could affect proteome diversity, we consider their structure, expression and relevance to biomedically significant processes or pathologies...
  12. pmc The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cells
    Chad A Galloway
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Ave, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 391:659-63. 2010
    ..The data suggested that additional regulatory mechanism(s) were induced by differentiation that controlled the functional activity of editing factors...
  13. pmc The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1
    Kefang Xie
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 101:8114-9. 2004
    ..Most importantly, the results suggested both AID and APOBEC-1 are equally likely to bind single-stranded DNA or RNA, which has implications for the identification of natural AID targets...
  14. ncbi request reprint Identification of novel alternative splice variants of APOBEC-1 complementation factor with different capacities to support apolipoprotein B mRNA editing
    Mark P Sowden
    Department of Biochemistry and Biophysics, University of Rochester, NY 14642, USA
    J Biol Chem 279:197-206. 2004
    ....
  15. pmc The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G
    James H Miller
    OyaGen, Inc, 601 Elmwood Ave, Rochester, NY 14642, USA
    Retrovirology 4:81. 2007
    ..Vif dimerization has been reported to be essential for viral infectivity but the mechanistic requirement for Vif multimerization is unknown...
  16. ncbi request reprint Measuring editing activity and identifying cytidine-to-uridine mRNA editing factors in cells and biochemical isolates
    Harold C Smith
    Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Methods Enzymol 424:389-416. 2007
    ..Practical approaches will be described for the measurement of editing activity and the analysis of proteins involved in C-to-U and dC-to-dU editing...
  17. pmc A hydrodynamic analysis of APOBEC3G reveals a monomer-dimer-tetramer self-association that has implications for anti-HIV function
    Jason D Salter
    Department of Biochemistry and Biophysics and Center for RNA Biology, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, New York 14642, USA
    Biochemistry 48:10685-7. 2009
    ..Overall, the results provide physical restraints for the A3G quaternary structure that have implications for modulating antiviral function...
  18. pmc Deaminase activity on single-stranded DNA (ssDNA) occurs in vitro when APOBEC3G cytidine deaminase forms homotetramers and higher-order complexes
    William M McDougall
    Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 286:30655-61. 2011
    ..8 S or 16 S complexes. We propose that deaminase-dependent antiviral activity of A3G in vivo may require a critical concentration of A3G in viral particles that will promote oligomerization on ssDNA during reverse transcription...
  19. pmc Nanostructures of APOBEC3G support a hierarchical assembly model of high molecular mass ribonucleoprotein particles from dimeric subunits
    Joseph E Wedekind
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    J Biol Chem 281:38122-6. 2006
    ..These observations imply that the disruption of cellular HMM particles may require regulation of protein-RNA, as well as protein-protein interactions, which has implications for therapeutic development...
  20. pmc Direct evidence that RNA inhibits APOBEC3G ssDNA cytidine deaminase activity
    William M McDougall
    Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
    Biochem Biophys Res Commun 412:612-7. 2011
    ..The data provided direct evidence that A3G binding to cellular RNAs constituted a substantial impediment to the enzyme's ability to interact with ssDNA...
  21. ncbi request reprint Activation induced deaminase: the importance of being specific
    Harold C Smith
    Department of Biochemistry and Biophysics, The James P Wilmot Cancer Center, USA
    Trends Genet 20:224-7. 2004
    ..Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate...
  22. ncbi request reprint A course director's perspectives on problem-based learning curricula in biochemistry
    Harold C Smith
    Medical school biochemistry, Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
    Acad Med 77:1189-98. 2002
    ..At the same time, complementary lectures greatly enhance uniformity in the quality and, importantly, the accuracy of the students' learning...
  23. ncbi request reprint Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase
    Chad A Galloway
    Department of Biochemistry and Biophysics, University of Rochester, 610 Elmwood Ave, Rochester, NY 14642, USA
    Biotechniques 34:524-6, 528, 530. 2003
    ..The principle factor that increased protein yield was the induction of protein expression in a late log phase culture, although reduced temperature during the induction and a low IPTG concentration also contributed to a higher yield...
  24. doi request reprint High-Affinity Recognition of HIV-1 Frameshift-Stimulating RNA Alters Frameshifting in Vitro and Interferes with HIV-1 Infectivity
    Leslie O Ofori
    Departments of Chemistry, Biochemistry and Biophysics, and Dermatology, University of Rochester, Rochester, New York 14642, United States
    J Med Chem 57:723-32. 2014
    ..These compounds are able to enhance frameshifting more than 50% in a dual-luciferase assay in human embryonic kidney cells, and they strongly inhibit the infectivity of pseudotyped HIV-1 virions. ..
  25. ncbi request reprint Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicing
    Geoffrey S C Dance
    Departments of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, USA
    J Biol Chem 277:12703-9. 2002
    ..The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression...
  26. ncbi request reprint Apolipoprotein B mRNA editing and the reduction in synthesis and secretion of the atherogenic risk factor, apolipoprotein B100 can be effectively targeted through TAT-mediated protein transduction
    Yan Yang
    Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    Mol Pharmacol 61:269-76. 2002
    ..These results suggested that apoB mRNA editing should be re-evaluated as a LDL-lowering therapeutic target in the new context of protein transduction therapy...