Research Topics
Genomes and Genes
| Harold C SmithSummaryAffiliation: University of Rochester Country: USA Publications
| Collaborators
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Detail Information
Publications
Functions and regulation of the APOBEC family of proteinsHarold C Smith
Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
Semin Cell Dev Biol 23:258-68. 2012....
APOBEC3G: a double agent in defenseHarold C Smith
Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, Rochester, NY 14642, USA
Trends Biochem Sci 36:239-44. 2011..Future research should focus on understanding how cellular and viral regulatory mechanisms enable the antiviral function of A3G, and on the development of novel research reagents to explore these pathways...- APOBEC3G subunits self-associate via the C-terminal deaminase domainRyan P Bennett
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Biol Chem 283:33329-36. 2008..These findings corroborate the small angle x-ray scattering structural model and are instructive for development of high throughput screens that target specific domains and their functions to identify HIV/AIDS therapeutics... - Functional characterization of APOBEC-1 complementation factor phosphorylation sitesDavid M Lehmann
Environmental Health Sciences Center, Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
Biochim Biophys Acta 1773:408-18. 2007..These data suggest that phosphorylation of ACF by PKC may be a key regulatory mechanism of apoB mRNA editing in rat hepatocytes... - Metabolic regulation of APOBEC-1 complementation factor trafficking in mouse models of obesity and its positive correlation with the expression of ApoB protein in hepatocytesChad A Galloway
University of Rochester, Department of Biochemistry and Biophysics, 601 Elmwood Ave Rochester, NY 14642, USA
Biochim Biophys Acta 1802:976-85. 2010.... 
Structural phylogenetic analysis of activation-induced deaminase functionH Travis Ichikawa
Department of Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
J Immunol 177:355-61. 2006....- Nuclear Exclusion of the HIV-1 host defense factor APOBEC3G requires a novel cytoplasmic retention signal and is not dependent on RNA bindingRyan P Bennett
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Biol Chem 283:7320-7. 2008..The CRS lies in a region involved in both Gag and Vif interactions; therefore, identification of this motif has important implications for the design of therapeutics that target HIV-1 while maintaining antiviral and cellular functions... - APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot trafficRyan P Bennett
Department of Biochemistry, Box 712, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
Biochem Biophys Res Commun 350:214-9. 2006..We demonstrate that hA3G is not a nucleo-cytoplasmic shuttling protein like APOBEC-1 and AID, but is strongly retained in the cytoplasm through a mechanism that involves both the N and C-terminal regions of the protein... - Metabolic regulation of apoB mRNA editing is associated with phosphorylation of APOBEC-1 complementation factorDavid M Lehmann
Department of Toxicology, University of Rochester, Rochester, NY 14642, USA
Nucleic Acids Res 34:3299-308. 2006.... - Messenger RNA editing in mammals: new members of the APOBEC family seeking roles in the family businessJoseph E Wedekind
Department of Biochemistry and Biophysics, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14623, USA
Trends Genet 19:207-16. 2003..In light of the hypothesis that these proteins might represent novel mRNA editing systems that could affect proteome diversity, we consider their structure, expression and relevance to biomedically significant processes or pathologies... - The editosome for cytidine to uridine mRNA editing has a native complexity of 27S: identification of intracellular domains containing active and inactive editing factorsMark P Sowden
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
J Cell Sci 115:1027-39. 2002..It is proposed that the heterogeneity in size of complexes containing editing factors is functionally significant and reflects functionally engaged editosomes in the nucleus and an inactive cytoplasmic pool of factors... - The expression of apoB mRNA editing factors is not the sole determinant for the induction of editing in differentiating Caco-2 cellsChad A Galloway
Department of Biochemistry and Biophysics, University of Rochester School of Medicine, 601 Elmwood Ave, Rochester, NY 14642, USA
Biochem Biophys Res Commun 391:659-63. 2010..The data suggested that additional regulatory mechanism(s) were induced by differentiation that controlled the functional activity of editing factors... - The structure of a yeast RNA-editing deaminase provides insight into the fold and function of activation-induced deaminase and APOBEC-1Kefang Xie
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
Proc Natl Acad Sci U S A 101:8114-9. 2004..Most importantly, the results suggested both AID and APOBEC-1 are equally likely to bind single-stranded DNA or RNA, which has implications for the identification of natural AID targets... - Identification of novel alternative splice variants of APOBEC-1 complementation factor with different capacities to support apolipoprotein B mRNA editingMark P Sowden
Department of Biochemistry and Biophysics, University of Rochester, NY 14642, USA
J Biol Chem 279:197-206. 2004.... - The dimerization domain of HIV-1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3GJames H Miller
OyaGen, Inc, 601 Elmwood Ave, Rochester, NY 14642, USA
Retrovirology 4:81. 2007..Vif dimerization has been reported to be essential for viral infectivity but the mechanistic requirement for Vif multimerization is unknown... - Measuring editing activity and identifying cytidine-to-uridine mRNA editing factors in cells and biochemical isolatesHarold C Smith
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Methods Enzymol 424:389-416. 2007..Practical approaches will be described for the measurement of editing activity and the analysis of proteins involved in C-to-U and dC-to-dU editing... - A hydrodynamic analysis of APOBEC3G reveals a monomer-dimer-tetramer self-association that has implications for anti-HIV functionJason D Salter
Department of Biochemistry and Biophysics and Center for RNA Biology, University of Rochester, 601 Elmwood Avenue, Box 712, Rochester, New York 14642, USA
Biochemistry 48:10685-7. 2009..Overall, the results provide physical restraints for the A3G quaternary structure that have implications for modulating antiviral function... - Deaminase activity on single-stranded DNA (ssDNA) occurs in vitro when APOBEC3G cytidine deaminase forms homotetramers and higher-order complexesWilliam M McDougall
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, New York 14642, USA
J Biol Chem 286:30655-61. 2011..8 S or 16 S complexes. We propose that deaminase-dependent antiviral activity of A3G in vivo may require a critical concentration of A3G in viral particles that will promote oligomerization on ssDNA during reverse transcription... - Nanostructures of APOBEC3G support a hierarchical assembly model of high molecular mass ribonucleoprotein particles from dimeric subunitsJoseph E Wedekind
Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
J Biol Chem 281:38122-6. 2006..These observations imply that the disruption of cellular HMM particles may require regulation of protein-RNA, as well as protein-protein interactions, which has implications for therapeutic development... - Activation induced deaminase: the importance of being specificHarold C Smith
Department of Biochemistry and Biophysics, The James P Wilmot Cancer Center, USA
Trends Genet 20:224-7. 2004..Recently, the biological relevance of assaying mammalian enzymes for DNA deaminase activity using Escherichia coli DNA as a reporter has been questioned, representing another round in the ongoing debate... - Direct evidence that RNA inhibits APOBEC3G ssDNA cytidine deaminase activityWilliam M McDougall
Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA
Biochem Biophys Res Commun 412:612-7. 2011..The data provided direct evidence that A3G binding to cellular RNAs constituted a substantial impediment to the enzyme's ability to interact with ssDNA... - A course director's perspectives on problem-based learning curricula in biochemistryHarold C Smith
Medical school biochemistry, Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York, USA
Acad Med 77:1189-98. 2002..At the same time, complementary lectures greatly enhance uniformity in the quality and, importantly, the accuracy of the students' learning... - Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phaseChad A Galloway
Department of Biochemistry and Biophysics, University of Rochester, 610 Elmwood Ave, Rochester, NY 14642, USA
Biotechniques 34:524-6, 528, 530. 2003..The principle factor that increased protein yield was the induction of protein expression in a late log phase culture, although reduced temperature during the induction and a low IPTG concentration also contributed to a higher yield... - Two proteins essential for apolipoprotein B mRNA editing are expressed from a single gene through alternative splicingGeoffrey S C Dance
Departments of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, USA
J Biol Chem 277:12703-9. 2002..The data account for the expression of two editing factors and provide a possible explanation for their different levels of expression... - Apolipoprotein B mRNA editing and the reduction in synthesis and secretion of the atherogenic risk factor, apolipoprotein B100 can be effectively targeted through TAT-mediated protein transductionYan Yang
Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
Mol Pharmacol 61:269-76. 2002..These results suggested that apoB mRNA editing should be re-evaluated as a LDL-lowering therapeutic target in the new context of protein transduction therapy...