JONATHAN M SLACK

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. ncbi request reprint In vitro transdifferentiation of hepatoma cells into functional pancreatic cells
    Wan Chun Li
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Mech Dev 122:835-47. 2005
  2. ncbi request reprint Copper deprivation in rats induces islet hyperplasia and hepatic metaplasia in the pancreas
    David Tosh
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Biol Cell 99:37-44. 2007
  3. ncbi request reprint Cell lineage tracing during Xenopus tail regeneration
    Cesare Gargioli
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Development 131:2669-79. 2004
  4. pmc Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors
    Wan Chun Li
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Int J Biochem Cell Biol 39:541-54. 2007
  5. ncbi request reprint Alternative cells for regeneration
    Jonathan M W Slack
    Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Cell 22:689-90. 2012
  6. pmc Regeneration of neural crest derivatives in the Xenopus tadpole tail
    Gufa Lin
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath, UK
    BMC Dev Biol 7:56. 2007
  7. ncbi request reprint Metaplasia and transdifferentiation: from pure biology to the clinic
    Jonathan M W Slack
    Centre for Regenerative Medicine, University of Bath, Bath BA27AY, UK
    Nat Rev Mol Cell Biol 8:369-78. 2007
  8. doi request reprint Metaplasia and somatic cell reprogramming
    J M W Slack
    Stem Cell Institute, University of Minnesota, MTRF, 2001 6th Street SE, Minneapolis, MN 55455, USA
    J Pathol 217:161-8. 2009
  9. ncbi request reprint The Xenopus tadpole: a new model for regeneration research
    J M W Slack
    Centre for Regenerative Medicine, University of Bath, Bath, BA2 7AY, United Kingdom
    Cell Mol Life Sci 65:54-63. 2008
  10. ncbi request reprint The spark of life: electricity and regeneration
    Jonathan M W Slack
    Stem Cell Institute, University of Minnesota, McGuire Translational Research Facility, 2001 6th Street SE, Minneapolis, MN 55455, USA
    Sci STKE 2007:pe54. 2007

Research Grants

  1. Making Beta Cells from Hepatocytes
    JONATHAN M SLACK; Fiscal Year: 2010
  2. Reprogramming Cells to Enable Limb Regeneration
    JONATHAN M SLACK; Fiscal Year: 2010

Collaborators

Detail Information

Publications33

  1. ncbi request reprint In vitro transdifferentiation of hepatoma cells into functional pancreatic cells
    Wan Chun Li
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Mech Dev 122:835-47. 2005
    ..The transdifferentiation of hepatic to pancreatic cells represents one possible source of beta-cells for human islet transplantation and this study shows that such a transdifferentiation can be achieved in vitro...
  2. ncbi request reprint Copper deprivation in rats induces islet hyperplasia and hepatic metaplasia in the pancreas
    David Tosh
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Biol Cell 99:37-44. 2007
    ..Prolonged copper deprivation in rats followed by refeeding with a normal diet has previously been used to induce the appearance of hepatocyte-like cells in the pancreas, but the effects on islet size and morphology have not been determined...
  3. ncbi request reprint Cell lineage tracing during Xenopus tail regeneration
    Cesare Gargioli
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Development 131:2669-79. 2004
    ..This shows that metaplasia between differentiated cell types does not occur, and that the process of Xenopus tail regeneration is more akin to tissue renewal in mammals than to urodele tail regeneration...
  4. pmc Keratinocyte serum-free medium maintains long-term liver gene expression and function in cultured rat hepatocytes by preventing the loss of liver-enriched transcription factors
    Wan Chun Li
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Int J Biochem Cell Biol 39:541-54. 2007
    ....
  5. ncbi request reprint Alternative cells for regeneration
    Jonathan M W Slack
    Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USA
    Dev Cell 22:689-90. 2012
    ..Normally, in fish fin regeneration, bone regenerates from bone. But what happens when there is no bone? Singh et al. (2012) show in this issue of Developmental Cell that the bony rays still regenerate from an alternative cell source...
  6. pmc Regeneration of neural crest derivatives in the Xenopus tadpole tail
    Gufa Lin
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath, UK
    BMC Dev Biol 7:56. 2007
    ..The regeneration of the neural crest derivatives has not previously been examined and is described in this paper...
  7. ncbi request reprint Metaplasia and transdifferentiation: from pure biology to the clinic
    Jonathan M W Slack
    Centre for Regenerative Medicine, University of Bath, Bath BA27AY, UK
    Nat Rev Mol Cell Biol 8:369-78. 2007
    ..This approach could generate new therapies for many human diseases...
  8. doi request reprint Metaplasia and somatic cell reprogramming
    J M W Slack
    Stem Cell Institute, University of Minnesota, MTRF, 2001 6th Street SE, Minneapolis, MN 55455, USA
    J Pathol 217:161-8. 2009
    ..It is proposed that analogous technology, using the appropriate transcription factors, could be used to bring about transformation to cell types other than embryonic stem cells...
  9. ncbi request reprint The Xenopus tadpole: a new model for regeneration research
    J M W Slack
    Centre for Regenerative Medicine, University of Bath, Bath, BA2 7AY, United Kingdom
    Cell Mol Life Sci 65:54-63. 2008
    ..The limb buds will regenerate completely at early stages, but not once they are fully differentiated. This provides a good opportunity to study the loss of regenerative ability using transgenic methods...
  10. ncbi request reprint The spark of life: electricity and regeneration
    Jonathan M W Slack
    Stem Cell Institute, University of Minnesota, McGuire Translational Research Facility, 2001 6th Street SE, Minneapolis, MN 55455, USA
    Sci STKE 2007:pe54. 2007
    ..This work could reopen a consideration of the role of electric fields in regeneration, an issue that has caused much controversy in the past...
  11. doi request reprint Origin of stem cells in organogenesis
    J M W Slack
    Stem Cell Institute, University of Minnesota, McGuire Translational Research Facility, 2001 6th Street SE, Minneapolis, MN 55455, USA
    Science 322:1498-501. 2008
    ..Possible explanations for the existence of such cells, and the issue of whether they also exist in vivo, are discussed...
  12. ncbi request reprint How cells change their phenotype
    David Tosh
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Nat Rev Mol Cell Biol 3:187-94. 2002
    ..This review examines recently discovered cases of metaplasia, and speculates on the potential molecular and cellular mechanisms that underlie the switches, and their significance to developmental biology and medicine...
  13. pmc All-trans retinoic acid suppresses exocrine differentiation and branching morphogenesis in the embryonic pancreas
    Chia Ning Shen
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Differentiation 75:62-74. 2007
    ....
  14. ncbi request reprint Amphibian muscle regeneration--dedifferentiation or satellite cells?
    Jonathan M W Slack
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Trends Cell Biol 16:273-5. 2006
    ..But recent work shows that muscle satellite cells are also involved in amphibian regeneration and the controversy about the reality of muscle dedifferentiation is heating up again...
  15. pmc Intestine in the lung
    Jonathan M W Slack
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    J Biol 3:10. 2004
    ..The transformation of lung to intestinal tissue has not previously been described, but it is now reported that it can be brought about by prolonged Wnt signaling in late development...
  16. ncbi request reprint Differentiated properties of hepatocytes induced from pancreatic cells
    David Tosh
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath, England
    Hepatology 36:534-43. 2002
    ..In conclusion, these results suggest that pancreatic hepatocytes produced by this method may offer an alternative model to primary cultures of hepatocytes for the study of liver function...
  17. pmc Glucocorticoids suppress beta-cell development and induce hepatic metaplasia in embryonic pancreas
    Chia Ning Shen
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Biochem J 375:41-50. 2003
    ..This mechanism may operate in combination with other changes during fetal programming, leading to type 2 diabetes in later life...
  18. doi request reprint The role of Cdx2 in Barrett's metaplasia
    Benjamin J Colleypriest
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Biochem Soc Trans 38:364-9. 2010
    ..In addition, Cdx2 is sufficient to provoke intestinal metaplasia in the stomach. In the present paper, we review the evidence for the role of Cdx2 in the development of Barrett's metaplasia...
  19. doi request reprint Barrett's metaplasia: molecular mechanisms and nutritional influences
    Jonathan M W Slack
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath BA2 7AY, U K
    Biochem Soc Trans 38:313-9. 2010
    ..It may also increase circulating levels of TNFalpha (tumour necrosis factor alpha), which activates Cdx2. In addition, there may be effects of diet on the composition of the bile...
  20. doi request reprint Reprogramming of liver to pancreas
    Shifaan Thowfeequ
    Centre for Regenerative Medicine, Department of Biology, University of Bath, Bath, UK
    Methods Mol Biol 482:407-18. 2009
    ..Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into beta-cells by the ectopic expression of critical beta-cell transcription factors...
  21. doi request reprint Requirement for Wnt and FGF signaling in Xenopus tadpole tail regeneration
    Gufa Lin
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Dev Biol 316:323-35. 2008
    ..The results show that the molecular mechanism of Xenopus tadpole tail regeneration is surprisingly similar to that of the Xenopus limb bud and the zebrafish caudal fin, despite the difference of anatomy...
  22. ncbi request reprint Transdifferentiation of pancreas to liver
    Chia Ning Shen
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, BA2 7AY Bath, UK
    Mech Dev 120:107-16. 2003
    ..The AR42J cell system has been used to elucidate the cell lineage and the molecular basis of transdifferentiation of pancreas to liver...
  23. ncbi request reprint Experimental conversion of liver to pancreas
    Marko E Horb
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, United Kingdom
    Curr Biol 13:105-15. 2003
    ..The aim of this study was to determine whether a gene overexpression protocol based on Pdx1 would be able to cause conversion of liver to pancreas...
  24. ncbi request reprint Regeneration-specific expression pattern of three posterior Hox genes
    Bea Christen
    Centre of Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
    Dev Dyn 226:349-55. 2003
    ..XHoxd13 is not expressed until relatively late and appears to be involved only in patterning of the blastema...
  25. ncbi request reprint Molecular pathways needed for regeneration of spinal cord and muscle in a vertebrate
    Caroline W Beck
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, BA2 7AY, Bath, United Kingdom
    Dev Cell 5:429-39. 2003
    ..The results demonstrate that regenerative capability can be enabled by genetic modifications that reactivate specific components of the developmental program...
  26. pmc Fission of pancreatic islets during postnatal growth of the mouse
    Philip A Seymour
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, UK
    J Anat 204:103-16. 2004
    ..This suggests that dumb-bell islets are in a state of fission rather than fusion, and that islet fission is a mode of islet production in the postnatal pancreas...
  27. ncbi request reprint Temporal requirement for bone morphogenetic proteins in regeneration of the tail and limb of Xenopus tadpoles
    Caroline W Beck
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom
    Mech Dev 123:674-88. 2006
    ....
  28. pmc In vitro culture of embryonic mouse intestinal epithelium: cell differentiation and introduction of reporter genes
    Jonathan M Quinlan
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    BMC Dev Biol 6:24. 2006
    ..The criteria for a reliable culture system include the ability to perform real time observations and manipulations in vitro, the preparation of wholemounts for immunostaining and the potential for introducing genes...
  29. ncbi request reprint Conversion of columnar to stratified squamous epithelium in the developing mouse oesophagus
    Wei Yuan Yu
    Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Claverton Down, Bath BA2 7AY, UK
    Dev Biol 284:157-70. 2005
    ..The results show that, in normal development, the squamous epithelium arises from the columnar epithelium by a direct conversion process...
  30. ncbi request reprint Control of muscle regeneration in the Xenopus tadpole tail by Pax7
    Ying Chen
    Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Development 133:2303-13. 2006
    ..This shows that inhibition of pax7 action does not prevent differentiation of satellite cells to myofibres, but it does prevent their maintenance as a stem cell population...
  31. ncbi request reprint Expression of amylase and other pancreatic genes in Xenopus
    Marko E Horb
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK
    Mech Dev 113:153-7. 2002
    ..In the endocrine cell clusters we do not find glucagon-expressing cells surrounding insulin-expressing cells, either in the tadpole or in the mature frog pancreas...
  32. ncbi request reprint Conversion of pancreatic cells to hepatocytes
    David Tosh
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, U K
    Biochem Soc Trans 30:51-5. 2002
    ..We also present evidence for (i) the molecular mechanism which regulates the conversion between pancreas and liver and (ii) the cellular basis of the switch in phenotype...
  33. ncbi request reprint Notch is required for outgrowth of the Xenopus tail bud
    Caroline W Beck
    Developmental Biology Programme, Department of Biology and Biochemistry, University of Bath, UK
    Int J Dev Biol 46:255-8. 2002
    ..This is achieved using inhibitors of the protease that carries out the ligand-induced intramembranous cleavage of Notch. Other protease inhibitors that do not inhibit Notch cleavage do not affect tail outgrowth...

Research Grants3

  1. Making Beta Cells from Hepatocytes
    JONATHAN M SLACK; Fiscal Year: 2010
    ..A new source of beta cells would enable many more transplants to be carried out. ..
  2. Reprogramming Cells to Enable Limb Regeneration
    JONATHAN M SLACK; Fiscal Year: 2010
    ..This innovative proposal will use modern stem cell technology to test a hypothesis about why this is so. If the hypothesis is confirmed, the methods developed should assist in facilitating the regeneration of mammalian limbs. ..