Research Topics
| Shivendra SinghSummaryAffiliation: University of Pittsburgh Country: USA Publications
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Publications
Cancer chemoprevention with dietary isothiocyanates mature for clinical translational researchShivendra V Singh
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Carcinogenesis 33:1833-42. 2012..Structure-activity relationship and mechanistic complexity in the context of cancer chemoprevention with ITCs is also highlighted...
Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse modelShivendra V Singh
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
J Natl Cancer Inst 104:1228-39. 2012..Phenethyl isothiocyanate (PEITC) is a natural plant compound with chemopreventative potential against some cancers and the ability to induce apoptosis in breast cancer cells...
Structural basis for catalytic differences between alpha class human glutathione transferases hGSTA1-1 and hGSTA2-2 for glutathione conjugation of environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxideShivendra V Singh
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Biochemistry 43:9708-15. 2004..Similar to (+)-anti-BPDE, however, the SIFS mutant was the most efficient enzyme for GSH conjugation of (-)-anti-BPDE...- Guggulsterone-induced apoptosis in human prostate cancer cells is caused by reactive oxygen intermediate dependent activation of c-Jun NH2-terminal kinaseShivendra V Singh
Department of Pharmacology and Urology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Cancer Res 67:7439-49. 2007..In conclusion, the present study reveals that the guggulsterone-induced cell death in human prostate cancer cells is regulated by ROI-dependent activation of JNK and guggulsterone inhibits promoter activity of androgen receptor... - Garlic constituent diallyl trisulfide prevents development of poorly differentiated prostate cancer and pulmonary metastasis multiplicity in TRAMP miceShivendra V Singh
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, PA, USA
Cancer Res 68:9503-11. 2008..In conclusion, the results of the present study show, for the first time, that DATS administration prevents progression to invasive carcinoma and lung metastasis in TRAMP mice... - Sulforaphane inhibits prostate carcinogenesis and pulmonary metastasis in TRAMP mice in association with increased cytotoxicity of natural killer cellsShivendra V Singh
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, USA
Cancer Res 69:2117-25. 2009..In conclusion, the results of the present study indicate that SFN administration inhibits prostate cancer progression and pulmonary metastasis in TRAMP mice by reducing cell proliferation and augmenting NK cell lytic activity... - Caspase-dependent apoptosis induction by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, in PC-3 human prostate cancer cells is mediated by Bax and BakShivendra V Singh
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, Pennsylvania 15213, USA
Mol Cancer Ther 4:1747-54. 2005..In conclusion, the present study indicates that caspase-dependent apoptosis by guggulsterone is mediated in part by Bax and Bak... - Bax and Bak are required for apoptosis induction by sulforaphane, a cruciferous vegetable-derived cancer chemopreventive agentSunga Choi
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Cancer Res 65:2035-43. 2005..In conclusion, the results of the present study indicate that Bax and Bak proteins play a critical role in initiation of cell death by sulforaphane... - Proteasome-mediated degradation of cell division cycle 25C and cyclin-dependent kinase 1 in phenethyl isothiocyanate-induced G2-M-phase cell cycle arrest in PC-3 human prostate cancer cellsDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Cancer Ther 3:567-75. 2004..Furthermore, the results of the present study point toward involvement of both caspase-8- and caspase-9-mediated pathways in apoptosis induction by PEITC... - Honokiol causes G0-G1 phase cell cycle arrest in human prostate cancer cells in association with suppression of retinoblastoma protein level/phosphorylation and inhibition of E2F1 transcriptional activityEun Ryeong Hahm
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Mol Cancer Ther 6:2686-95. 2007.... - D,L-Sulforaphane-induced cell death in human prostate cancer cells is regulated by inhibitor of apoptosis family proteins and Apaf-1Sunga Choi
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Carcinogenesis 28:151-62. 2007..In conclusion, the present study reveals a complex signaling mechanism involving Bax activation, downregulation of IAP family proteins and Apaf-1 induction in regulation of SFN-induced cell death... - Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G2/M arrest and inducing apoptosisDong Xiao
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, PA 15213, USA
Carcinogenesis 24:891-7. 2003..In conclusion, it seems reasonable to hypothesize that AITC, and possibly other ITCs, may find use in the treatment of human prostate cancers... - Caspase-dependent apoptosis induction by phenethyl isothiocyanate, a cruciferous vegetable-derived cancer chemopreventive agent, is mediated by Bak and BaxDong Xiao
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Clin Cancer Res 11:2670-9. 2005..CONCLUSION: The results of the present study indicate that caspase-dependent apoptosis by PEITC is mediated by Bak and Bax proteins... - Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1-dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlicAnna Herman-Antosiewicz
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Mol Cancer Ther 6:1249-61. 2007..In conclusion, the present study describes a novel signaling pathway involving ATR/Chk1 in the regulation of DATS-induced prometaphase arrest... - p66Shc is indispensable for phenethyl isothiocyanate-induced apoptosis in human prostate cancer cellsDong Xiao
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Cancer Res 70:3150-8. 2010..Collectively, these results provide novel insight into the critical role of p66(Shc) in regulation of PEITC-induced apoptotic cell death in human prostate cancer cells... - Diallyl trisulfide-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by reactive oxygen species-dependent destruction and hyperphosphorylation of Cdc 25 CDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Oncogene 24:6256-68. 2005..In conclusion, the results of the present study indicate that DATS-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by ROS-mediated destruction and hyperphosphorylation of Cdc 25 C... - c-Jun NH(2)-terminal kinase signaling axis regulates diallyl trisulfide-induced generation of reactive oxygen species and cell cycle arrest in human prostate cancer cellsJedrzej Antosiewicz
Department of Pharmacology and Urology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Cancer Res 66:5379-86. 2006..In conclusion, the present study provides experimental evidence to indicate existence of a novel pathway involving JNK signaling axis in regulation of DATS-induced ROS generation... - Diallyl trisulfide, a constituent of processed garlic, inactivates Akt to trigger mitochondrial translocation of BAD and caspase-mediated apoptosis in human prostate cancer cellsDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
Carcinogenesis 27:533-40. 2006..In conclusion, the present study demonstrates that DATS-induced apoptosis in human prostate cancer cells is mediated, at least in part, by inactivation of Akt signaling axis... - Plumbagin-induced apoptosis in human prostate cancer cells is associated with modulation of cellular redox status and generation of reactive oxygen speciesAnna A Powolny
Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Pharm Res 25:2171-80. 2008..To investigate the mechanism of human prostate cancer cell growth inhibition by plumbagin, a constituent of the widely used medicinal herb Plumbago zeylanica L... - Diallyl trisulfide selectively causes Bax- and Bak-mediated apoptosis in human lung cancer cellsDong Xiao
Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Environ Mol Mutagen 50:201-12. 2009..On the other hand, BID protein was dispensable for DATS-induced apoptosis. In conclusion, the present study indicates that Bax and Bak proteins are critical targets of DATS-induced apoptosis in human lung cancer cells... - Orphan nuclear receptor pregnane X receptor sensitizes oxidative stress responses in transgenic mice and cancerous cellsHaibiao Gong
Center for Pharmacogenetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
Mol Endocrinol 20:279-90. 2006..The current study reveals a novel function of PXR in the mammalian oxidative stress response, and this regulatory pathway may be implicated in carcinogenesis by sensitizing normal and cancerous tissues to oxidative cellular damage... - Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak inductionDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
Clin Cancer Res 12:6836-43. 2006..CONCLUSIONS: The present study indicates that DATS administration inhibits growth of PC-3 xenografts in vivo in association with induction of Bax and Bak... - Mitochondria-mediated apoptosis by diallyl trisulfide in human prostate cancer cells is associated with generation of reactive oxygen species and regulated by Bax/BakYoung Ae Kim
Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Cancer Ther 6:1599-609. 2007..In conclusion, the present study reveals that the mitochondria-mediated cell death by DATS is associated with ROS generation and regulated by Bax/Bak but independent of Bcl-2 or Bcl-xL... - Checkpoint kinase 1 regulates diallyl trisulfide-induced mitotic arrest in human prostate cancer cellsAnna Herman-Antosiewicz
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 280:28519-28. 2005..In conclusion, the results of the present study suggest existence of a checkpoint kinase 1-dependent mechanism for diallyl trisulfide-induced mitotic arrest in human prostate cancer cells... - Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cellsEun Ryeong Hahm
2 32A Hillman Cancer Center Research Pavilion, University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
Cancer Prev Res (Phila) 3:484-94. 2010..Suppression of STAT3 activation was also observed in cells treated with naturally occurring analogues of SFN. In conclusion, the present study indicates that inhibition of STAT3 partially contributes to the proapoptotic effect of SFN... - Cellular responses to cancer chemopreventive agent D,L-sulforaphane in human prostate cancer cells are initiated by mitochondrial reactive oxygen speciesDong Xiao
Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
Pharm Res 26:1729-38. 2009..Present study was undertaken to elucidate the mechanism of cellular responses to D,L-sulforaphane (SFN), a highly promising cancer chemopreventive agent... - Phenethyl isothiocyanate-induced apoptosis in p53-deficient PC-3 human prostate cancer cell line is mediated by extracellular signal-regulated kinasesDong Xiao
Department of Pharmacology and the University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
Cancer Res 62:3615-9. 2002..Thus, it seems reasonable to postulate that PEITC may be effective against tumors with normal as well as mutant p53... - Transcriptional repression and inhibition of nuclear translocation of androgen receptor by diallyl trisulfide in human prostate cancer cellsSilvia D Stan
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Clin Cancer Res 15:4895-903. 2009..The present study was undertaken to determine the effect of diallyl trisulfide (DATS), a promising cancer chemopreventive constituent of garlic, on androgen receptor (AR) protein expression and function using prostate cancer cells... - Diallyl trisulfide-induced G2/M phase cell cycle arrest in DU145 cells is associated with delayed nuclear translocation of cyclin-dependent kinase 1Anna Herman-Antosiewicz
Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
Pharm Res 27:1072-9. 2010..The present study was undertaken to gain insight into the molecular mechanism of G2/M phase cell cycle arrest resulting from treatment of DU145 cells with diallyl trisulfide (DATS), a promising cancer chemopreventive constituent of garlic... - Phenethyl isothiocyanate-induced apoptosis in PC-3 human prostate cancer cells is mediated by reactive oxygen species-dependent disruption of the mitochondrial membrane potentialDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine Pittsburgh, PA, USA
Carcinogenesis 27:2223-34. 2006..In conclusion, the present study indicates that the PEITC-induced apoptosis in PC-3 cells is mediated by ROS-dependent disruption of the mitochondrial membrane potential and regulated by Bax and Bid... - Ayurvedic medicine constituent withaferin a causes G2 and M phase cell cycle arrest in human breast cancer cellsSilvia D Stan
University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Nutr Cancer 60:51-60. 2008..In conclusion, the results of this study suggest that G2-M phase cell cycle arrest may be an important mechanism in antiproliferative effect of WA against human breast cancer cells... - Benzyl isothiocyanate-induced apoptosis in human breast cancer cells is initiated by reactive oxygen species and regulated by Bax and BakDong Xiao
2.32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
Mol Cancer Ther 5:2931-45. 2006..In conclusion, the present study reveals a complex signaling leading to growth arrest and apoptosis induction by BITC... - Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits growth of PC-3 human prostate cancer xenografts in vivoSanjay K Srivastava
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Carcinogenesis 24:1665-70. 2003..In conclusion, the results of the present study indicate that AITC administration inhibits growth of PC-3 xenografts in vivo by inducing apoptosis and reducing mitotic activity... - p53 regulates cellular responses to environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxide in human lung cancer cellsHui Xiao
Department of Pharmacology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
Cell Cycle 6:1753-61. 2007..In conclusion, the present study points towards an important role of p53 in regulation of cellular responses to BPDE in human lung cancer cells... - Diallyl trisulfide-induced apoptosis in human cancer cells is linked to checkpoint kinase 1-mediated mitotic arrestDong Xiao
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Mol Carcinog 48:1018-29. 2009.... - p53-Independent apoptosis by benzyl isothiocyanate in human breast cancer cells is mediated by suppression of XIAP expressionSu Hyeong Kim
Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pennsylvania, USA
Cancer Prev Res (Phila) 3:718-26. 2010..In conclusion, the present study provides novel insight into the molecular circuitry of BITC-induced apoptosis to indicate suppression of XIAP expression as a critical mediator of this process... - Multitargeted prevention and therapy of cancer by diallyl trisulfide and related Allium vegetable-derived organosulfur compoundsAnna A Powolny
Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Cancer Lett 269:305-14. 2008..This article summarizes preclinical and limited clinical data to warrant further clinical evaluation of Allium vegetable constituents for prevention and therapy of human cancers... - Atg5 regulates phenethyl isothiocyanate-induced autophagic and apoptotic cell death in human prostate cancer cellsAjay Bommareddy
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, Pennsylvania, USA
Cancer Res 69:3704-12. 2009..In conclusion, the present study indicates that Atg5 plays an important role in regulation of PEITC-induced autophagic and apoptotic cell death... - Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2Dong Xiao
Department of Pharmacology and Medicine, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Oncogene 23:5594-606. 2004..In conclusion, our data point towards important roles for Bcl-2, JNK and ERK in DATS-induced apoptosis in human prostate cancer cells... - D,L-Sulforaphane causes transcriptional repression of androgen receptor in human prostate cancer cellsSu Hyeong Kim
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Mol Cancer Ther 8:1946-54. 2009..In conclusion, the present study shows for the first time that SFN treatment causes transcriptional repression of AR and inhibition of its nuclear localization in human prostate cancer cells... - Guggulsterone enhances head and neck cancer therapies via inhibition of signal transducer and activator of transcription-3Rebecca J Leeman-Neill
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
Carcinogenesis 30:1848-56. 2009..Our results suggest that guggulsterone-mediated inhibition of STAT3 and HIF-1alpha provide a biologic rationale for further clinical investigation of this compound in the treatment of HNSCC... - Prevention of mammary carcinogenesis in MMTV-neu mice by cruciferous vegetable constituent benzyl isothiocyanateRenaud Warin
Department of Pharmacology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cancer Res 69:9473-80. 2009..The present study demonstrating efficacy of BITC against mammary cancer in an animal model provides impetus to determine its activity in a clinical setting... - Phenethyl isothiocyanate inhibits oxidative phosphorylation to trigger reactive oxygen species-mediated death of human prostate cancer cellsDong Xiao
Department of Pharmacology and Chemical Biology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 285:26558-69. 2010..In conclusion, the present study provides novel insight into the molecular circuitry of PEITC-induced cell death involving ROS production due to inhibition of complex III and OXPHOS... - Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2Dong Xiao
University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Nutr Cancer 55:94-107. 2006..The present study demonstrates, for the first time, that DATS has the ability to inhibit angiogenic features of human endothelial cells... - Benzyl isothiocyanate targets mitochondrial respiratory chain to trigger reactive oxygen species-dependent apoptosis in human breast cancer cellsDong Xiao
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
J Biol Chem 283:30151-63. 2008..In conclusion, the present study indicates that the BITC-induced apoptosis in human breast cancer cells is initiated by mitochondria-derived ROS... - Inhibition of EGFR-STAT3 signaling with erlotinib prevents carcinogenesis in a chemically-induced mouse model of oral squamous cell carcinomaRebecca J Leeman-Neill
Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
Cancer Prev Res (Phila) 4:230-7. 2011..The results of the present study provide the basis for exploring the efficacy of erlotinib for prevention of HNSCC in a clinical setting... - Reactive oxygen species-dependent apoptosis by gugulipid extract of Ayurvedic medicine plant Commiphora mukul in human prostate cancer cells is regulated by c-Jun N-terminal kinaseDong Xiao
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Mol Pharmacol 79:499-507. 2011..In conclusion, the present study indicates that ROS-dependent apoptosis by GL is regulated by JNK signaling axis... - Honokiol, a constituent of oriental medicinal herb magnolia officinalis, inhibits growth of PC-3 xenografts in vivo in association with apoptosis inductionEun Ryeong Hahm
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Clin Cancer Res 14:1248-57. 2008..This study was undertaken to determine the efficacy of honokiol, a constituent of oriental medicinal herb Magnolia officinalis, against human prostate cancer cells in culture and in vivo... - Chemoprevention strategies for pancreatic cancerSilvia D Stan
Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Pittsburgh School of Medicine, 5117 Centre Avenue, 2 31 Hillman Cancer Center, Pittsburgh, PA 15213, USA
Nat Rev Gastroenterol Hepatol 7:347-56. 2010..This Review discusses the current state of the pancreatic cancer chemoprevention field and highlights the challenges ahead... - Sulforaphane-induced G2/M phase cell cycle arrest involves checkpoint kinase 2-mediated phosphorylation of cell division cycle 25CShivendra V Singh
Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
J Biol Chem 279:25813-22. 2004..Activation of Chk2 in response to DNA damage is well documented, but the present study is the first published report to link Chk2 activation to cell cycle arrest by an isothiocyanate... - Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivoAjita V Singh
Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Carcinogenesis 25:83-90. 2004..To the best of our knowledge, the present study is the first published report to document in vivo anticancer activity of SFN in a tumor xenograft model... - Requirement of a carbon spacer in benzyl isothiocyanate-mediated cytotoxicity and MAPK activation in head and neck squamous cell carcinomaVivian W Y Lui
Department of Otolaryngology and 2Pharmacology, University of Pittsburgh School of Medicine and the University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
Carcinogenesis 24:1705-12. 2003..Our results show that minor structural differences in ITCs can be crucial for the antiproliferative activity of ITCs and that BITC may be a promising chemopreventive as well as therapeutic agent in HNSCC... - Sulforaphane-induced cell death in human prostate cancer cells is initiated by reactive oxygen speciesShivendra V Singh
Department of Pharmacology and University of Pittsburgh Cancer Institute, Pennsylvania 15213, USA
J Biol Chem 280:19911-24. 2005.... - The carcinogen (7R,8S)-dihydroxy-(9S,10R)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induces Cdc25B expression in human bronchial and lung cancer cellsTetsuya Oguri
Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
Cancer Res 63:771-5. 2003..We postulate that the induction of Cdc25B expression in lung cancer cells by the ultimate carcinogen anti-BPDE accelerates the further development of lung carcinogenesis... - Sulforaphane causes autophagy to inhibit release of cytochrome C and apoptosis in human prostate cancer cellsAnna Herman-Antosiewicz
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cancer Res 66:5828-35. 2006..To the best of our knowledge, the present study is the first published report to convincingly document induction of autophagy by an isothiocyanate class of dietary chemopreventive agent... - Cell cycle arrest, apoptosis induction and inhibition of nuclear factor kappa B activation in anti-proliferative activity of benzyl isothiocyanate against human pancreatic cancer cellsSanjay K Srivastava
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Carcinogenesis 25:1701-9. 2004..To the best of our knowledge, this study is the first published report to implicate suppression of NF-kappaB activation as a potential mechanism for anti-proliferative activity of BITC against human pancreatic cancer cells... - Phenethyl isothiocyanate, a cancer chemopreventive constituent of cruciferous vegetables, inhibits cap-dependent translation by regulating the level and phosphorylation of 4E-BP1Jing Hu
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
Cancer Res 67:3569-73. 2007..The present study also suggests that inhibition of cap-dependent translation may be an important mechanism in PEITC-induced apoptosis... - Cell division cycle 25B phosphatase is essential for benzo(a)pyrene-7,8-Diol-9,10-epoxide induced neoplastic transformationSanjay K Srivastava
Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
Cancer Res 67:9150-7. 2007..We conclude that Cdc25B has an essential role in anti-BPDE-induced neoplastic transformation, including regulation of cell cycle resumption in the presence of DNA damage... - Withaferin A causes FOXO3a- and Bim-dependent apoptosis and inhibits growth of human breast cancer cells in vivoSilvia D Stan
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cancer Res 68:7661-9. 2008..These results point toward an important role of FOXO3a and Bim in regulation of WA-mediated apoptosis in human breast cancer cells... - Inhibition of human breast cancer xenograft growth by cruciferous vegetable constituent benzyl isothiocyanateRenaud Warin
Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Mol Carcinog 49:500-7. 2010..These preclinical observations merit clinical investigation to determine efficacy of BITC against human breast cancers... - Withaferin A inhibits activation of signal transducer and activator of transcription 3 in human breast cancer cellsJoomin Lee
Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
Carcinogenesis 31:1991-8. 2010.... - Benzo[a]pyrene-7,8-diol-9,10-epoxide causes caspase-mediated apoptosis in H460 human lung cancer cell lineHui Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cell Cycle 6:2826-34. 2007..In conclusion, the present study reveals that BPDE-induced apoptosis in H460 cells is associated with Bak induction and caspase activation but independent of Bcl-2... - Exceptional activity of murine glutathione transferase A1-1 against (7R,8S)-dihydroxy-(9S,10R)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-induced DNA damage in stably transfected cellsJianxia Guo
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Carcinog 36:67-73. 2003..The results of the present study indicated that mGSTA1-1 was exceptionally effective in affording protection against (+)-anti-BPDE-induced DNA damage in a cellular system... - z-Guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, inhibits angiogenesis in vitro and in vivoDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, School of Medicine, Pittsburgh, PA 15213, USA
Mol Cancer Ther 7:171-80. 2008..Together, our results provide compelling rationale for further preclinical and clinical investigation of z-guggulsterone for its efficacy against prostate cancer... - Phenethyl isothiocyanate inhibits angiogenesis in vitro and ex vivoDong Xiao
Department of Pharmacology and University Cancer Institute, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
Cancer Res 67:2239-46. 2007..In conclusion, the present study suggests that inhibition of angiogenesis may be an important mechanism in cancer chemoprevention by PEITC... - Role of mitogen-activated protein kinases in phenethyl isothiocyanate-induced apoptosis in human prostate cancer cellsDong Xiao
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
Mol Carcinog 43:130-40. 2005..These results indicate that involvement of MAPKs in apoptosis induction by PEITC in human prostate cancer cells is cell line-specific... - Garlic constituent diallyl trisulfide suppresses x-linked inhibitor of apoptosis protein in prostate cancer cells in culture and in vivoSu Hyeong Kim
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pennsylvania, USA
Cancer Prev Res (Phila) 4:897-906. 2011.... - Benzyl isothiocyanate inhibits epithelial-mesenchymal transition in cultured and xenografted human breast cancer cellsAnuradha Sehrawat
Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
Cancer Prev Res (Phila) 4:1107-17. 2011..In conclusion, this study reports a novel anticancer effect of BITC involving inhibition of EMT, a process triggered during progression of cancer to invasive state... - Chemopreventative potential of the cruciferous vegetable constituent phenethyl isothiocyanate in a mouse model of prostate cancerAnna A Powolny
Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
J Natl Cancer Inst 103:571-84. 2011.... - Critical role of allyl groups and disulfide chain in induction of Pi class glutathione transferase in mouse tissues in vivo by diallyl disulfide, a naturally occurring chemopreventive agent in garlicChhanda Bose
Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
Carcinogenesis 23:1661-5. 2002..In conclusion, the results of the present study clearly indicate that the presence of terminal allyl groups as well as the central disulfide chain is required for maximum induction of mGSTP1 in vivo by garlic-derived OSCs... - Role of RLIP76 in lung cancer doxorubicin resistance: I. The ATPase activity of RLIP76 correlates with doxorubicin and 4-hydroxynonenal resistance in lung cancer cellsSharad S Singhal
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019, USA
Int J Oncol 22:365-75. 2003..Taken together, these findings suggest that RLIP76 activity is a general determinant of 4HNE and DOX resistance, and that its activity contributes to the drug-resistant phenotype of NSCLC... - Protein kinase D3 (PKD3) contributes to prostate cancer cell growth and survival through a PKCepsilon/PKD3 pathway downstream of Akt and ERK 1/2Jun Chen
Departments of Pharmacology, and Urology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
Cancer Res 68:3844-53. 2008..In summary, our data indicate that PKD3 contributes to growth and survival of prostate cancer cells and may represent a novel therapeutic target for prostate cancer... - Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxideYijun Gu
Macromolecular Crystallographic Laboratory, National Cancer Institute, Frederick, Maryland 21702, USA
Biochemistry 42:917-21. 2003..Comparison of the two structures suggests that residues 207 and 221 are responsible for the different binding mode of GSBpd and therefore contribute to the distinct catalytic efficiency of the two isozymes... - JS-K, a glutathione/glutathione S-transferase-activated nitric oxide donor of the diazeniumdiolate class with potent antineoplastic activityPaul J Shami
Division of Medical Oncology, Department of Internal Medicine, University of Utah and Salt Lake City Veterans Administration Medical Centers, Salt Lake City, Utah 84148, USA
Mol Cancer Ther 2:409-17. 2003..Similar results were seen with s.c. human prostate cancer (PPC-1) xenografts. Our data indicate that JS-K is a promising lead compound for the possible development of a novel class of antineoplastic agents... - Induction of p21 protein protects against sulforaphane-induced mitotic arrest in LNCaP human prostate cancer cell lineAnna Herman-Antosiewicz
Department of Molecular Biology, University of Gdask, Gdask, Poland
Mol Cancer Ther 6:1673-81. 2007..In conclusion, the present study indicates that induction of p21 protects against SFN-induced mitotic arrest in LNCaP cells... - Crystal structure of human glutathione S-transferase A3-3 and mechanistic implications for its high steroid isomerase activityYijun Gu
Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland 21702, USA
Biochemistry 43:15673-9. 2004.... - Role of glutathione conjugate efflux in cellular protection against benzo[a]pyrene-7,8-diol-9,10-epoxide-induced DNA damageSanjay K Srivastava
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
Mol Carcinog 33:156-62. 2002..The results of the present study indicate that in addition to GSH conjugation, the efflux of BPD-SG may be essential for cellular protection against (+)-anti-BPDE-induced DNA damage... - Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cellsRajendra Sharma
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 0647, USA
Eur J Biochem 271:1690-701. 2004..These studies suggest that alterations in 4-HNE homeostasis can profoundly affect cell-cycle signaling events... - Cloning and expression of a novel Mu class murine glutathione transferase isoenzymeJianxia Guo
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, S 871 Scaife Hall Box 130, PA 15261, USA
Biochem J 366:817-24. 2002..Reverse transcription-PCR analysis using mGSTM7-specific primers revealed that mGSTM7 is widely expressed in tissues of female A/J mice, including liver, forestomach, lung, kidney, colon and spleen... - Transport functions and physiological significance of 76 kDa Ral-binding GTPase activating protein (RLIP76)Sanjay Awasthi
Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX, USA
Acta Biochim Pol 49:855-67. 2002..In this article, we discuss the significance of transport functions of RLIP76 highlighting its role in the defense mechanisms against oxidative injury, and modulation of signaling mechanisms... - Signal transduction pathways leading to cell cycle arrest and apoptosis induction in cancer cells by Allium vegetable-derived organosulfur compounds: a reviewAnna Herman-Antosiewicz
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Suite 2.32A Hillman Cancer Center Research Pavilion, 5117 Center Avenue, Pittsburgh, PA 15213, USA
Mutat Res 555:121-31. 2004..This review summarizes current knowledge on signal transduction pathways leading to perturbations in cell cycle progression and apoptosis induction by OSCs... - Differential expression of glutathione S-transferase isoenzymes in murine small intestine and colonJianxia Guo
Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
Comp Biochem Physiol B Biochem Mol Biol 131:443-52. 2002..1 and GST5.5 could not be detected in the colon. The results of the present study indicate that the small intestine of female A/J mice is better equipped for protection against toxic effects of electrophiles than colon...
Research Grants
- Anticarcinogenic Effect of ITCs Against Prostate CancerShivendra Singh; Fiscal Year: 2009..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
- Breast Cancer Prevention by Ayurvedic Medicine ConstituentsShivendra Singh; Fiscal Year: 2011..In summary, the hypothesis-driven and mechanistically-focused studies proposed in this application will provide preclinical data necessary for clinical development of WA as a chemopreventive agent against breast cancer in humans. ..
- Breast Cancer Prevention by Dietary PhytochemicalsShivendra Singh; Fiscal Year: 2007....
- Anticarcinogenic Effect of ITCs Against Prostate CancerShivendra Singh; Fiscal Year: 2007..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
- NOVEL GST AND DETOXICATION OF DIOL EPOXIDESShivendra Singh; Fiscal Year: 2006..In the long-term, this knowledge will guide us in developing strategies for prevention of PAH-induced cancers in humans. ..
- Anticarcinogenic Effect of ITCs Against Prostate CancerShivendra Singh; Fiscal Year: 2011..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
- Prostate Cancer Prevention by Diallyl TrisulfideShivendra Singh; Fiscal Year: 2010..In summary, the immediate and future intrinsic value of the studies proposed in this renewal application resides in optimization of DATS-based regimens for chemoprevention of prostate cancer. ..
- Breast Cancer Prevention by Dietary PhytochemicalsShivendra Singh; Fiscal Year: 2010....
- Breast Cancer Prevention by Ayurvedic Medicine ConstituentsShivendra Singh; Fiscal Year: 2010..In summary, the hypothesis-driven and mechanistically-focused studies proposed in this application will provide preclinical data necessary for clinical development of WA as a chemopreventive agent against breast cancer in humans. ..
- Anticarcinogenic Effect of ITCs Against Prostate CancerShivendra Singh; Fiscal Year: 2010..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
- Prevention of Prostate Cancer by SulforaphaneShivendra Singh; Fiscal Year: 2009..abstract_text> ..
- Prostate Cancer Prevention by Diallyl TrisulfideShivendra Singh; Fiscal Year: 2009..In the long run, the results of the proposed studies could lead to DATS-based strategies for prevention and/or treatment of human prostate cancers. ..
- Toxicological Relevance of Human GST P1-1 PolymorphismShivendra Singh; Fiscal Year: 2005..abstract_text> ..
- NOVEL GST AND DETOXICATION OF DIOL EPOXIDESShivendra Singh; Fiscal Year: 2001..5 and its orthologue(s) in the detoxication of these carcinogens. In the long term, strategies to manipulate this group of GSTs to the advantage of cells/organ should be extremely important for cancer prevention. ..
- TOXICOLOGICAL RELEVANCE OF HUMAN GST PL-L POLYMORPHISMShivendra Singh; Fiscal Year: 2001..The population will consist of members of both genders and will include Caucasians, blacks and other minorities, as available and representative of the patient base at Mercy Hospital. ..
- NOVEL GST AND DETOXICATION OF DIOL EPOXIDESShivendra Singh; Fiscal Year: 2003..In the long-term, this knowledge will guide us in developing strategies for prevention of PAH-induced cancers in humans. ..
- MECHANISM OF ANTICARCINOGENIC ACTIVITY--ORGANOSULFIDESShivendra Singh; Fiscal Year: 2003..Insights gained from these studies should help in devising practical dietary recommendation for humans. ..
- ROLE OF HUMAN GST PI POLYMORPHISM IN DRUG RESISTANCEShivendra Singh; Fiscal Year: 2003..These studies, in the long term, may be valuable in identifying subjects that may be at a higher risk for developing GST-mediated resistance to some alkylating agents. ..