Shivendra Singh

Summary

Affiliation: University of Pittsburgh
Country: USA

Publications

  1. pmc Benzyl isothiocyanate causes FoxO1-mediated autophagic death in human breast cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e32597. 2012
  2. pmc Notch activation by phenethyl isothiocyanate attenuates its inhibitory effect on prostate cancer cell migration
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e26615. 2011
  3. pmc Critical role of p53 upregulated modulator of apoptosis in benzyl isothiocyanate-induced apoptotic cell death
    Marie Lue Antony
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e32267. 2012
  4. pmc Withaferin A-induced apoptosis in human breast cancer cells is mediated by reactive oxygen species
    Eun Ryeong Hahm
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e23354. 2011
  5. pmc Notch activation is dispensable for D, L-sulforaphane-mediated inhibition of human prostate cancer cell migration
    Eun Ryeong Hahm
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e44957. 2012
  6. pmc Autophagy fails to alter withaferin A-mediated lethality in human breast cancer cells
    Eun Ryeong Hahm
    2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Curr Cancer Drug Targets 13:640-50. 2013
  7. pmc Dietary chemopreventative benzyl isothiocyanate inhibits breast cancer stem cells in vitro and in vivo
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Prev Res (Phila) 6:782-90. 2013
  8. pmc Cancer chemoprevention with dietary isothiocyanates mature for clinical translational research
    Shivendra V Singh
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Carcinogenesis 33:1833-42. 2012
  9. pmc Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model
    Shivendra V Singh
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Natl Cancer Inst 104:1228-39. 2012
  10. ncbi request reprint Caspase-dependent apoptosis induction by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, in PC-3 human prostate cancer cells is mediated by Bax and Bak
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, Pennsylvania 15213, USA
    Mol Cancer Ther 4:1747-54. 2005

Research Grants

  1. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2009
  2. Breast Cancer Prevention by Ayurvedic Medicine Constituents
    Shivendra Singh; Fiscal Year: 2011
  3. Breast Cancer Prevention by Dietary Phytochemicals
    Shivendra Singh; Fiscal Year: 2007
  4. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2007
  5. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2007
  6. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2007
  7. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2006
  8. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2006
  9. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2006
  10. NOVEL GST AND DETOXICATION OF DIOL EPOXIDES
    Shivendra Singh; Fiscal Year: 2006

Collaborators

Detail Information

Publications89

  1. pmc Benzyl isothiocyanate causes FoxO1-mediated autophagic death in human breast cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e32597. 2012
    ..In conclusion, the present study provides novel insights into the molecular circuitry of BITC-induced cell death involving FoxO1-mediated autophagy...
  2. pmc Notch activation by phenethyl isothiocyanate attenuates its inhibitory effect on prostate cancer cell migration
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e26615. 2011
    ..Because Notch signaling is implicated in epithelial-mesenchymal transition and metastasis, the present study suggests that anti-metastatic effect of PEITC may be augmented by a combination regimen involving a Notch inhibitor...
  3. pmc Critical role of p53 upregulated modulator of apoptosis in benzyl isothiocyanate-induced apoptotic cell death
    Marie Lue Antony
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e32267. 2012
    ..In conclusion, the results of the present study indicate that Bim-independent apoptosis by BITC in cancer cells is mediated by PUMA...
  4. pmc Withaferin A-induced apoptosis in human breast cancer cells is mediated by reactive oxygen species
    Eun Ryeong Hahm
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 6:e23354. 2011
    ..In conclusion, the present study provides novel insight into the molecular circuitry of WA-induced apoptosis involving ROS production and activation of Bax/Bak...
  5. pmc Notch activation is dispensable for D, L-sulforaphane-mediated inhibition of human prostate cancer cell migration
    Eun Ryeong Hahm
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America
    PLoS ONE 7:e44957. 2012
    ..These results indicate that Notch activation is largely dispensable for SFN-mediated inhibition of cell migration, which should be viewed as a therapeutic advantage as Notch activation is frequent in human prostate cancers...
  6. pmc Autophagy fails to alter withaferin A-mediated lethality in human breast cancer cells
    Eun Ryeong Hahm
    2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Curr Cancer Drug Targets 13:640-50. 2013
    ....
  7. pmc Dietary chemopreventative benzyl isothiocyanate inhibits breast cancer stem cells in vitro and in vivo
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Prev Res (Phila) 6:782-90. 2013
    ..Ron overexpression resulted in upregulation of bCSC-associated genes Oct-4, SOX-2, and Nanog. In conclusion, the present study indicates that BITC treatment eliminates bCSCs in vitro and in vivo...
  8. pmc Cancer chemoprevention with dietary isothiocyanates mature for clinical translational research
    Shivendra V Singh
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Carcinogenesis 33:1833-42. 2012
    ..Structure-activity relationship and mechanistic complexity in the context of cancer chemoprevention with ITCs is also highlighted...
  9. pmc Biomarkers of phenethyl isothiocyanate-mediated mammary cancer chemoprevention in a clinically relevant mouse model
    Shivendra V Singh
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    J Natl Cancer Inst 104:1228-39. 2012
    ..Phenethyl isothiocyanate (PEITC) is a natural plant compound with chemopreventative potential against some cancers and the ability to induce apoptosis in breast cancer cells...
  10. ncbi request reprint Caspase-dependent apoptosis induction by guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, in PC-3 human prostate cancer cells is mediated by Bax and Bak
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, Pennsylvania 15213, USA
    Mol Cancer Ther 4:1747-54. 2005
    ..In conclusion, the present study indicates that caspase-dependent apoptosis by guggulsterone is mediated in part by Bax and Bak...
  11. ncbi request reprint Structural basis for catalytic differences between alpha class human glutathione transferases hGSTA1-1 and hGSTA2-2 for glutathione conjugation of environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxide
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Biochemistry 43:9708-15. 2004
    ..Similar to (+)-anti-BPDE, however, the SIFS mutant was the most efficient enzyme for GSH conjugation of (-)-anti-BPDE...
  12. ncbi request reprint Guggulsterone-induced apoptosis in human prostate cancer cells is caused by reactive oxygen intermediate dependent activation of c-Jun NH2-terminal kinase
    Shivendra V Singh
    Department of Pharmacology and Urology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 67:7439-49. 2007
    ..In conclusion, the present study reveals that the guggulsterone-induced cell death in human prostate cancer cells is regulated by ROI-dependent activation of JNK and guggulsterone inhibits promoter activity of androgen receptor...
  13. pmc Sulforaphane inhibits prostate carcinogenesis and pulmonary metastasis in TRAMP mice in association with increased cytotoxicity of natural killer cells
    Shivendra V Singh
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, USA
    Cancer Res 69:2117-25. 2009
    ..In conclusion, the results of the present study indicate that SFN administration inhibits prostate cancer progression and pulmonary metastasis in TRAMP mice by reducing cell proliferation and augmenting NK cell lytic activity...
  14. pmc Garlic constituent diallyl trisulfide prevents development of poorly differentiated prostate cancer and pulmonary metastasis multiplicity in TRAMP mice
    Shivendra V Singh
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, PA, USA
    Cancer Res 68:9503-11. 2008
    ..In conclusion, the results of the present study show, for the first time, that DATS administration prevents progression to invasive carcinoma and lung metastasis in TRAMP mice...
  15. ncbi request reprint Bax and Bak are required for apoptosis induction by sulforaphane, a cruciferous vegetable-derived cancer chemopreventive agent
    Sunga Choi
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 65:2035-43. 2005
    ..In conclusion, the results of the present study indicate that Bax and Bak proteins play a critical role in initiation of cell death by sulforaphane...
  16. ncbi request reprint Proteasome-mediated degradation of cell division cycle 25C and cyclin-dependent kinase 1 in phenethyl isothiocyanate-induced G2-M-phase cell cycle arrest in PC-3 human prostate cancer cells
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Cancer Ther 3:567-75. 2004
    ..Furthermore, the results of the present study point toward involvement of both caspase-8- and caspase-9-mediated pathways in apoptosis induction by PEITC...
  17. ncbi request reprint D,L-Sulforaphane-induced cell death in human prostate cancer cells is regulated by inhibitor of apoptosis family proteins and Apaf-1
    Sunga Choi
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Carcinogenesis 28:151-62. 2007
    ..In conclusion, the present study reveals a complex signaling mechanism involving Bax activation, downregulation of IAP family proteins and Apaf-1 induction in regulation of SFN-induced cell death...
  18. ncbi request reprint Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits proliferation of human prostate cancer cells by causing G2/M arrest and inducing apoptosis
    Dong Xiao
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, PA 15213, USA
    Carcinogenesis 24:891-7. 2003
    ..In conclusion, it seems reasonable to hypothesize that AITC, and possibly other ITCs, may find use in the treatment of human prostate cancers...
  19. ncbi request reprint Caspase-dependent apoptosis induction by phenethyl isothiocyanate, a cruciferous vegetable-derived cancer chemopreventive agent, is mediated by Bak and Bax
    Dong Xiao
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Clin Cancer Res 11:2670-9. 2005
    ....
  20. ncbi request reprint Honokiol causes G0-G1 phase cell cycle arrest in human prostate cancer cells in association with suppression of retinoblastoma protein level/phosphorylation and inhibition of E2F1 transcriptional activity
    Eun Ryeong Hahm
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 6:2686-95. 2007
    ....
  21. ncbi request reprint Activation of a novel ataxia-telangiectasia mutated and Rad3 related/checkpoint kinase 1-dependent prometaphase checkpoint in cancer cells by diallyl trisulfide, a promising cancer chemopreventive constituent of processed garlic
    Anna Herman-Antosiewicz
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Cancer Ther 6:1249-61. 2007
    ..In conclusion, the present study describes a novel signaling pathway involving ATR/Chk1 in the regulation of DATS-induced prometaphase arrest...
  22. pmc p66Shc is indispensable for phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 70:3150-8. 2010
    ..Collectively, these results provide novel insight into the critical role of p66(Shc) in regulation of PEITC-induced apoptotic cell death in human prostate cancer cells...
  23. ncbi request reprint Diallyl trisulfide-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by reactive oxygen species-dependent destruction and hyperphosphorylation of Cdc 25 C
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Oncogene 24:6256-68. 2005
    ..In conclusion, the results of the present study indicate that DATS-induced G(2)-M phase cell cycle arrest in human prostate cancer cells is caused by ROS-mediated destruction and hyperphosphorylation of Cdc 25 C...
  24. ncbi request reprint c-Jun NH(2)-terminal kinase signaling axis regulates diallyl trisulfide-induced generation of reactive oxygen species and cell cycle arrest in human prostate cancer cells
    Jedrzej Antosiewicz
    Department of Pharmacology and Urology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Cancer Res 66:5379-86. 2006
    ..In conclusion, the present study provides experimental evidence to indicate existence of a novel pathway involving JNK signaling axis in regulation of DATS-induced ROS generation...
  25. ncbi request reprint Plumbagin-induced apoptosis in human prostate cancer cells is associated with modulation of cellular redox status and generation of reactive oxygen species
    Anna A Powolny
    Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Pharm Res 25:2171-80. 2008
    ..To investigate the mechanism of human prostate cancer cell growth inhibition by plumbagin, a constituent of the widely used medicinal herb Plumbago zeylanica L...
  26. ncbi request reprint Diallyl trisulfide, a constituent of processed garlic, inactivates Akt to trigger mitochondrial translocation of BAD and caspase-mediated apoptosis in human prostate cancer cells
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA
    Carcinogenesis 27:533-40. 2006
    ..In conclusion, the present study demonstrates that DATS-induced apoptosis in human prostate cancer cells is mediated, at least in part, by inactivation of Akt signaling axis...
  27. ncbi request reprint Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with Bax and Bak induction
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    Clin Cancer Res 12:6836-43. 2006
    ..The present study was undertaken to determine the effect of garlic constituent diallyl trisulfide (DATS) on growth of PC-3 human prostate cancer xenograft in vivo...
  28. pmc Diallyl trisulfide selectively causes Bax- and Bak-mediated apoptosis in human lung cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Environ Mol Mutagen 50:201-12. 2009
    ..On the other hand, BID protein was dispensable for DATS-induced apoptosis. In conclusion, the present study indicates that Bax and Bak proteins are critical targets of DATS-induced apoptosis in human lung cancer cells...
  29. pmc Transcriptional repression and inhibition of nuclear translocation of androgen receptor by diallyl trisulfide in human prostate cancer cells
    Silvia D Stan
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Clin Cancer Res 15:4895-903. 2009
    ..The present study was undertaken to determine the effect of diallyl trisulfide (DATS), a promising cancer chemopreventive constituent of garlic, on androgen receptor (AR) protein expression and function using prostate cancer cells...
  30. pmc Cellular responses to cancer chemopreventive agent D,L-sulforaphane in human prostate cancer cells are initiated by mitochondrial reactive oxygen species
    Dong Xiao
    Department of Pharmacology and Chemical Biology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA
    Pharm Res 26:1729-38. 2009
    ..Present study was undertaken to elucidate the mechanism of cellular responses to D,L-sulforaphane (SFN), a highly promising cancer chemopreventive agent...
  31. pmc Mitochondria-mediated apoptosis by diallyl trisulfide in human prostate cancer cells is associated with generation of reactive oxygen species and regulated by Bax/Bak
    Young Ae Kim
    Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Cancer Ther 6:1599-609. 2007
    ..In conclusion, the present study reveals that the mitochondria-mediated cell death by DATS is associated with ROS generation and regulated by Bax/Bak but independent of Bcl-2 or Bcl-xL...
  32. ncbi request reprint Orphan nuclear receptor pregnane X receptor sensitizes oxidative stress responses in transgenic mice and cancerous cells
    Haibiao Gong
    Center for Pharmacogenetics, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Mol Endocrinol 20:279-90. 2006
    ..The current study reveals a novel function of PXR in the mammalian oxidative stress response, and this regulatory pathway may be implicated in carcinogenesis by sensitizing normal and cancerous tissues to oxidative cellular damage...
  33. ncbi request reprint Checkpoint kinase 1 regulates diallyl trisulfide-induced mitotic arrest in human prostate cancer cells
    Anna Herman-Antosiewicz
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 280:28519-28. 2005
    ..In conclusion, the results of the present study suggest existence of a checkpoint kinase 1-dependent mechanism for diallyl trisulfide-induced mitotic arrest in human prostate cancer cells...
  34. ncbi request reprint Phenethyl isothiocyanate-induced apoptosis in p53-deficient PC-3 human prostate cancer cell line is mediated by extracellular signal-regulated kinases
    Dong Xiao
    Department of Pharmacology and the University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Cancer Res 62:3615-9. 2002
    ..Thus, it seems reasonable to postulate that PEITC may be effective against tumors with normal as well as mutant p53...
  35. pmc Sulforaphane inhibits constitutive and interleukin-6-induced activation of signal transducer and activator of transcription 3 in prostate cancer cells
    Eun Ryeong Hahm
    2 32A Hillman Cancer Center Research Pavilion, University of Pittsburgh Cancer Institute, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Cancer Prev Res (Phila) 3:484-94. 2010
    ..Suppression of STAT3 activation was also observed in cells treated with naturally occurring analogues of SFN. In conclusion, the present study indicates that inhibition of STAT3 partially contributes to the proapoptotic effect of SFN...
  36. pmc Diallyl trisulfide-induced G2/M phase cell cycle arrest in DU145 cells is associated with delayed nuclear translocation of cyclin-dependent kinase 1
    Anna Herman-Antosiewicz
    Department of Pharmacology and Chemical Biology University of Pittsburgh School of Medicine, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    Pharm Res 27:1072-9. 2010
    ..The present study was undertaken to gain insight into the molecular mechanism of G2/M phase cell cycle arrest resulting from treatment of DU145 cells with diallyl trisulfide (DATS), a promising cancer chemopreventive constituent of garlic...
  37. pmc Ayurvedic medicine constituent withaferin a causes G2 and M phase cell cycle arrest in human breast cancer cells
    Silvia D Stan
    University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Nutr Cancer 60:51-60. 2008
    ..In conclusion, the results of this study suggest that G2-M phase cell cycle arrest may be an important mechanism in antiproliferative effect of WA against human breast cancer cells...
  38. ncbi request reprint Allyl isothiocyanate, a constituent of cruciferous vegetables, inhibits growth of PC-3 human prostate cancer xenografts in vivo
    Sanjay K Srivastava
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Carcinogenesis 24:1665-70. 2003
    ..In conclusion, the results of the present study indicate that AITC administration inhibits growth of PC-3 xenografts in vivo by inducing apoptosis and reducing mitotic activity...
  39. pmc Diallyl trisulfide-induced apoptosis in human cancer cells is linked to checkpoint kinase 1-mediated mitotic arrest
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Carcinog 48:1018-29. 2009
    ....
  40. pmc p53-Independent apoptosis by benzyl isothiocyanate in human breast cancer cells is mediated by suppression of XIAP expression
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Cancer Prev Res (Phila) 3:718-26. 2010
    ..In conclusion, the present study provides novel insight into the molecular circuitry of BITC-induced apoptosis to indicate suppression of XIAP expression as a critical mediator of this process...
  41. ncbi request reprint p53 regulates cellular responses to environmental carcinogen benzo[a]pyrene-7,8-diol-9,10-epoxide in human lung cancer cells
    Hui Xiao
    Department of Pharmacology, University of Pittsburgh Cancer Institute, Pittsburgh, Pennsylvania 15213, USA
    Cell Cycle 6:1753-61. 2007
    ..In conclusion, the present study points towards an important role of p53 in regulation of cellular responses to BPDE in human lung cancer cells...
  42. ncbi request reprint Benzyl isothiocyanate-induced apoptosis in human breast cancer cells is initiated by reactive oxygen species and regulated by Bax and Bak
    Dong Xiao
    2 32A Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Mol Cancer Ther 5:2931-45. 2006
    ..In conclusion, the present study reveals a complex signaling leading to growth arrest and apoptosis induction by BITC...
  43. ncbi request reprint Phenethyl isothiocyanate-induced apoptosis in PC-3 human prostate cancer cells is mediated by reactive oxygen species-dependent disruption of the mitochondrial membrane potential
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine Pittsburgh, PA, USA
    Carcinogenesis 27:2223-34. 2006
    ..In conclusion, the present study indicates that the PEITC-induced apoptosis in PC-3 cells is mediated by ROS-dependent disruption of the mitochondrial membrane potential and regulated by Bax and Bid...
  44. ncbi request reprint Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-Jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2
    Dong Xiao
    Department of Pharmacology and Medicine, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Oncogene 23:5594-606. 2004
    ..In conclusion, our data point towards important roles for Bcl-2, JNK and ERK in DATS-induced apoptosis in human prostate cancer cells...
  45. pmc Prevention of mammary carcinogenesis in MMTV-neu mice by cruciferous vegetable constituent benzyl isothiocyanate
    Renaud Warin
    Department of Pharmacology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Res 69:9473-80. 2009
    ..The present study demonstrating efficacy of BITC against mammary cancer in an animal model provides impetus to determine its activity in a clinical setting...
  46. ncbi request reprint Diallyl trisulfide inhibits angiogenic features of human umbilical vein endothelial cells by causing Akt inactivation and down-regulation of VEGF and VEGF-R2
    Dong Xiao
    University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Nutr Cancer 55:94-107. 2006
    ..The present study demonstrates, for the first time, that DATS has the ability to inhibit angiogenic features of human endothelial cells...
  47. pmc D,L-Sulforaphane causes transcriptional repression of androgen receptor in human prostate cancer cells
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Mol Cancer Ther 8:1946-54. 2009
    ..In conclusion, the present study shows for the first time that SFN treatment causes transcriptional repression of AR and inhibition of its nuclear localization in human prostate cancer cells...
  48. pmc Atg5 regulates phenethyl isothiocyanate-induced autophagic and apoptotic cell death in human prostate cancer cells
    Ajay Bommareddy
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, Pennsylvania, USA
    Cancer Res 69:3704-12. 2009
    ..In conclusion, the present study indicates that Atg5 plays an important role in regulation of PEITC-induced autophagic and apoptotic cell death...
  49. pmc Phenethyl isothiocyanate inhibits oxidative phosphorylation to trigger reactive oxygen species-mediated death of human prostate cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 285:26558-69. 2010
    ..In conclusion, the present study provides novel insight into the molecular circuitry of PEITC-induced cell death involving ROS production due to inhibition of complex III and OXPHOS...
  50. pmc Guggulsterone enhances head and neck cancer therapies via inhibition of signal transducer and activator of transcription-3
    Rebecca J Leeman-Neill
    Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15261, USA
    Carcinogenesis 30:1848-56. 2009
    ..Our results suggest that guggulsterone-mediated inhibition of STAT3 and HIF-1alpha provide a biologic rationale for further clinical investigation of this compound in the treatment of HNSCC...
  51. pmc Multitargeted prevention and therapy of cancer by diallyl trisulfide and related Allium vegetable-derived organosulfur compounds
    Anna A Powolny
    Department of Pharmacology and Urology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Cancer Lett 269:305-14. 2008
    ..This article summarizes preclinical and limited clinical data to warrant further clinical evaluation of Allium vegetable constituents for prevention and therapy of human cancers...
  52. pmc Reactive oxygen species-dependent apoptosis by gugulipid extract of Ayurvedic medicine plant Commiphora mukul in human prostate cancer cells is regulated by c-Jun N-terminal kinase
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Mol Pharmacol 79:499-507. 2011
    ..In conclusion, the present study indicates that ROS-dependent apoptosis by GL is regulated by JNK signaling axis...
  53. pmc Inhibition of EGFR-STAT3 signaling with erlotinib prevents carcinogenesis in a chemically-induced mouse model of oral squamous cell carcinoma
    Rebecca J Leeman-Neill
    Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Cancer Prev Res (Phila) 4:230-7. 2011
    ..The results of the present study provide the basis for exploring the efficacy of erlotinib for prevention of HNSCC in a clinical setting...
  54. pmc Chemoprevention strategies for pancreatic cancer
    Silvia D Stan
    Division of Gastroenterology, Hepatology and Nutrition, Department of Medicine, University of Pittsburgh School of Medicine, 5117 Centre Avenue, 2 31 Hillman Cancer Center, Pittsburgh, PA 15213, USA
    Nat Rev Gastroenterol Hepatol 7:347-56. 2010
    ..This Review discusses the current state of the pancreatic cancer chemoprevention field and highlights the challenges ahead...
  55. pmc Benzyl isothiocyanate targets mitochondrial respiratory chain to trigger reactive oxygen species-dependent apoptosis in human breast cancer cells
    Dong Xiao
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    J Biol Chem 283:30151-63. 2008
    ..In conclusion, the present study indicates that the BITC-induced apoptosis in human breast cancer cells is initiated by mitochondria-derived ROS...
  56. ncbi request reprint Sulforaphane-induced G2/M phase cell cycle arrest involves checkpoint kinase 2-mediated phosphorylation of cell division cycle 25C
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    J Biol Chem 279:25813-22. 2004
    ..Activation of Chk2 in response to DNA damage is well documented, but the present study is the first published report to link Chk2 activation to cell cycle arrest by an isothiocyanate...
  57. ncbi request reprint Sulforaphane-induced cell death in human prostate cancer cells is initiated by reactive oxygen species
    Shivendra V Singh
    Department of Pharmacology and University of Pittsburgh Cancer Institute, Pennsylvania 15213, USA
    J Biol Chem 280:19911-24. 2005
    ....
  58. ncbi request reprint Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo
    Ajita V Singh
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    Carcinogenesis 25:83-90. 2004
    ..To the best of our knowledge, the present study is the first published report to document in vivo anticancer activity of SFN in a tumor xenograft model...
  59. ncbi request reprint Phenethyl isothiocyanate, a cancer chemopreventive constituent of cruciferous vegetables, inhibits cap-dependent translation by regulating the level and phosphorylation of 4E-BP1
    Jing Hu
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Hillman Cancer Center Research Pavilion, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Cancer Res 67:3569-73. 2007
    ..The present study also suggests that inhibition of cap-dependent translation may be an important mechanism in PEITC-induced apoptosis...
  60. ncbi request reprint Cell cycle arrest, apoptosis induction and inhibition of nuclear factor kappa B activation in anti-proliferative activity of benzyl isothiocyanate against human pancreatic cancer cells
    Sanjay K Srivastava
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Carcinogenesis 25:1701-9. 2004
    ..To the best of our knowledge, this study is the first published report to implicate suppression of NF-kappaB activation as a potential mechanism for anti-proliferative activity of BITC against human pancreatic cancer cells...
  61. ncbi request reprint Sulforaphane causes autophagy to inhibit release of cytochrome C and apoptosis in human prostate cancer cells
    Anna Herman-Antosiewicz
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Res 66:5828-35. 2006
    ..To the best of our knowledge, the present study is the first published report to convincingly document induction of autophagy by an isothiocyanate class of dietary chemopreventive agent...
  62. ncbi request reprint Cell division cycle 25B phosphatase is essential for benzo(a)pyrene-7,8-Diol-9,10-epoxide induced neoplastic transformation
    Sanjay K Srivastava
    Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15260, USA
    Cancer Res 67:9150-7. 2007
    ..We conclude that Cdc25B has an essential role in anti-BPDE-induced neoplastic transformation, including regulation of cell cycle resumption in the presence of DNA damage...
  63. ncbi request reprint Requirement of a carbon spacer in benzyl isothiocyanate-mediated cytotoxicity and MAPK activation in head and neck squamous cell carcinoma
    Vivian W Y Lui
    Department of Otolaryngology and 2Pharmacology, University of Pittsburgh School of Medicine and the University of Pittsburgh Cancer Institute, Pittsburgh, PA 15213, USA
    Carcinogenesis 24:1705-12. 2003
    ..Our results show that minor structural differences in ITCs can be crucial for the antiproliferative activity of ITCs and that BITC may be a promising chemopreventive as well as therapeutic agent in HNSCC...
  64. ncbi request reprint The carcinogen (7R,8S)-dihydroxy-(9S,10R)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induces Cdc25B expression in human bronchial and lung cancer cells
    Tetsuya Oguri
    Department of Pharmacology, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA
    Cancer Res 63:771-5. 2003
    ..We postulate that the induction of Cdc25B expression in lung cancer cells by the ultimate carcinogen anti-BPDE accelerates the further development of lung carcinogenesis...
  65. doi request reprint Honokiol, a constituent of oriental medicinal herb magnolia officinalis, inhibits growth of PC-3 xenografts in vivo in association with apoptosis induction
    Eun Ryeong Hahm
    Department of Pharmacology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Clin Cancer Res 14:1248-57. 2008
    ..This study was undertaken to determine the efficacy of honokiol, a constituent of oriental medicinal herb Magnolia officinalis, against human prostate cancer cells in culture and in vivo...
  66. pmc Withaferin A causes FOXO3a- and Bim-dependent apoptosis and inhibits growth of human breast cancer cells in vivo
    Silvia D Stan
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Res 68:7661-9. 2008
    ..These results point toward an important role of FOXO3a and Bim in regulation of WA-mediated apoptosis in human breast cancer cells...
  67. ncbi request reprint Exceptional activity of murine glutathione transferase A1-1 against (7R,8S)-dihydroxy-(9S,10R)-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene-induced DNA damage in stably transfected cells
    Jianxia Guo
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Carcinog 36:67-73. 2003
    ..The results of the present study indicated that mGSTA1-1 was exceptionally effective in affording protection against (+)-anti-BPDE-induced DNA damage in a cellular system...
  68. pmc Inhibition of human breast cancer xenograft growth by cruciferous vegetable constituent benzyl isothiocyanate
    Renaud Warin
    Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Mol Carcinog 49:500-7. 2010
    ..These preclinical observations merit clinical investigation to determine efficacy of BITC against human breast cancers...
  69. pmc Withaferin A inhibits activation of signal transducer and activator of transcription 3 in human breast cancer cells
    Joomin Lee
    Department of Pharmacology and Chemical Biology, and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Carcinogenesis 31:1991-8. 2010
    ....
  70. ncbi request reprint Benzo[a]pyrene-7,8-diol-9,10-epoxide causes caspase-mediated apoptosis in H460 human lung cancer cell line
    Hui Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cell Cycle 6:2826-34. 2007
    ..In conclusion, the present study reveals that BPDE-induced apoptosis in H460 cells is associated with Bak induction and caspase activation but independent of Bcl-2...
  71. doi request reprint z-Guggulsterone, a constituent of Ayurvedic medicinal plant Commiphora mukul, inhibits angiogenesis in vitro and in vivo
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, School of Medicine, Pittsburgh, PA 15213, USA
    Mol Cancer Ther 7:171-80. 2008
    ..Together, our results provide compelling rationale for further preclinical and clinical investigation of z-guggulsterone for its efficacy against prostate cancer...
  72. ncbi request reprint Phenethyl isothiocyanate inhibits angiogenesis in vitro and ex vivo
    Dong Xiao
    Department of Pharmacology and University Cancer Institute, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA 15213, USA
    Cancer Res 67:2239-46. 2007
    ..In conclusion, the present study suggests that inhibition of angiogenesis may be an important mechanism in cancer chemoprevention by PEITC...
  73. ncbi request reprint Role of mitogen-activated protein kinases in phenethyl isothiocyanate-induced apoptosis in human prostate cancer cells
    Dong Xiao
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15213, USA
    Mol Carcinog 43:130-40. 2005
    ..These results indicate that involvement of MAPKs in apoptosis induction by PEITC in human prostate cancer cells is cell line-specific...
  74. pmc Benzyl isothiocyanate inhibits epithelial-mesenchymal transition in cultured and xenografted human breast cancer cells
    Anuradha Sehrawat
    Department of Pharmacology and Chemical Biology, University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA
    Cancer Prev Res (Phila) 4:1107-17. 2011
    ..In conclusion, this study reports a novel anticancer effect of BITC involving inhibition of EMT, a process triggered during progression of cancer to invasive state...
  75. pmc Garlic constituent diallyl trisulfide suppresses x-linked inhibitor of apoptosis protein in prostate cancer cells in culture and in vivo
    Su Hyeong Kim
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pennsylvania, USA
    Cancer Prev Res (Phila) 4:897-906. 2011
    ....
  76. pmc Chemopreventative potential of the cruciferous vegetable constituent phenethyl isothiocyanate in a mouse model of prostate cancer
    Anna A Powolny
    Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
    J Natl Cancer Inst 103:571-84. 2011
    ....
  77. ncbi request reprint JS-K, a glutathione/glutathione S-transferase-activated nitric oxide donor of the diazeniumdiolate class with potent antineoplastic activity
    Paul J Shami
    Division of Medical Oncology, Department of Internal Medicine, University of Utah and Salt Lake City Veterans Administration Medical Centers, Salt Lake City, Utah 84148, USA
    Mol Cancer Ther 2:409-17. 2003
    ..Similar results were seen with s.c. human prostate cancer (PPC-1) xenografts. Our data indicate that JS-K is a promising lead compound for the possible development of a novel class of antineoplastic agents...
  78. ncbi request reprint Critical role of allyl groups and disulfide chain in induction of Pi class glutathione transferase in mouse tissues in vivo by diallyl disulfide, a naturally occurring chemopreventive agent in garlic
    Chhanda Bose
    Department of Medicine, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA
    Carcinogenesis 23:1661-5. 2002
    ..In conclusion, the results of the present study clearly indicate that the presence of terminal allyl groups as well as the central disulfide chain is required for maximum induction of mGSTP1 in vivo by garlic-derived OSCs...
  79. ncbi request reprint Role of RLIP76 in lung cancer doxorubicin resistance: I. The ATPase activity of RLIP76 correlates with doxorubicin and 4-hydroxynonenal resistance in lung cancer cells
    Sharad S Singhal
    Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX 76019, USA
    Int J Oncol 22:365-75. 2003
    ..Taken together, these findings suggest that RLIP76 activity is a general determinant of 4HNE and DOX resistance, and that its activity contributes to the drug-resistant phenotype of NSCLC...
  80. ncbi request reprint Residues 207, 216, and 221 and the catalytic activity of mGSTA1-1 and mGSTA2-2 toward benzo[a]pyrene-(7R,8S)-diol-(9S,10R)-epoxide
    Yijun Gu
    Macromolecular Crystallographic Laboratory, National Cancer Institute, Frederick, Maryland 21702, USA
    Biochemistry 42:917-21. 2003
    ..Comparison of the two structures suggests that residues 207 and 221 are responsible for the different binding mode of GSBpd and therefore contribute to the distinct catalytic efficiency of the two isozymes...
  81. doi request reprint Protein kinase D3 (PKD3) contributes to prostate cancer cell growth and survival through a PKCepsilon/PKD3 pathway downstream of Akt and ERK 1/2
    Jun Chen
    Departments of Pharmacology, and Urology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Cancer Res 68:3844-53. 2008
    ..In summary, our data indicate that PKD3 contributes to growth and survival of prostate cancer cells and may represent a novel therapeutic target for prostate cancer...
  82. ncbi request reprint Crystal structure of human glutathione S-transferase A3-3 and mechanistic implications for its high steroid isomerase activity
    Yijun Gu
    Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland 21702, USA
    Biochemistry 43:15673-9. 2004
    ....
  83. ncbi request reprint Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells
    Rajendra Sharma
    Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX 77555 0647, USA
    Eur J Biochem 271:1690-701. 2004
    ..These studies suggest that alterations in 4-HNE homeostasis can profoundly affect cell-cycle signaling events...
  84. ncbi request reprint Induction of p21 protein protects against sulforaphane-induced mitotic arrest in LNCaP human prostate cancer cell line
    Anna Herman-Antosiewicz
    Department of Molecular Biology, University of Gdask, Gdask, Poland
    Mol Cancer Ther 6:1673-81. 2007
    ..In conclusion, the present study indicates that induction of p21 protects against SFN-induced mitotic arrest in LNCaP cells...
  85. ncbi request reprint Role of glutathione conjugate efflux in cellular protection against benzo[a]pyrene-7,8-diol-9,10-epoxide-induced DNA damage
    Sanjay K Srivastava
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Carcinog 33:156-62. 2002
    ..The results of the present study indicate that in addition to GSH conjugation, the efflux of BPD-SG may be essential for cellular protection against (+)-anti-BPDE-induced DNA damage...
  86. ncbi request reprint Transport functions and physiological significance of 76 kDa Ral-binding GTPase activating protein (RLIP76)
    Sanjay Awasthi
    Department of Chemistry and Biochemistry, University of Texas at Arlington, Arlington, TX, USA
    Acta Biochim Pol 49:855-67. 2002
    ..In this article, we discuss the significance of transport functions of RLIP76 highlighting its role in the defense mechanisms against oxidative injury, and modulation of signaling mechanisms...
  87. pmc Cloning and expression of a novel Mu class murine glutathione transferase isoenzyme
    Jianxia Guo
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, S 871 Scaife Hall Box 130, PA 15261, USA
    Biochem J 366:817-24. 2002
    ..Reverse transcription-PCR analysis using mGSTM7-specific primers revealed that mGSTM7 is widely expressed in tissues of female A/J mice, including liver, forestomach, lung, kidney, colon and spleen...
  88. ncbi request reprint Differential expression of glutathione S-transferase isoenzymes in murine small intestine and colon
    Jianxia Guo
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Comp Biochem Physiol B Biochem Mol Biol 131:443-52. 2002
    ..1 and GST5.5 could not be detected in the colon. The results of the present study indicate that the small intestine of female A/J mice is better equipped for protection against toxic effects of electrophiles than colon...
  89. ncbi request reprint Signal transduction pathways leading to cell cycle arrest and apoptosis induction in cancer cells by Allium vegetable-derived organosulfur compounds: a review
    Anna Herman-Antosiewicz
    Department of Pharmacology and University of Pittsburgh Cancer Institute, University of Pittsburgh School of Medicine, Suite 2 32A Hillman Cancer Center Research Pavilion, 5117 Center Avenue, Pittsburgh, PA 15213, USA
    Mutat Res 555:121-31. 2004
    ..This review summarizes current knowledge on signal transduction pathways leading to perturbations in cell cycle progression and apoptosis induction by OSCs...

Research Grants45

  1. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2009
    ..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
  2. Breast Cancer Prevention by Ayurvedic Medicine Constituents
    Shivendra Singh; Fiscal Year: 2011
    ..In summary, the hypothesis-driven and mechanistically-focused studies proposed in this application will provide preclinical data necessary for clinical development of WA as a chemopreventive agent against breast cancer in humans. ..
  3. Breast Cancer Prevention by Dietary Phytochemicals
    Shivendra Singh; Fiscal Year: 2007
    ....
  4. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2007
    ..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
  5. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2007
    ..abstract_text> ..
  6. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2007
    ..In the long run, the results of the proposed studies could lead to DATS-based strategies for prevention and/or treatment of human prostate cancers. ..
  7. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2006
    ..abstract_text> ..
  8. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2006
    ..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
  9. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2006
    ..In the long run, the results of the proposed studies could lead to DATS-based strategies for prevention and/or treatment of human prostate cancers. ..
  10. NOVEL GST AND DETOXICATION OF DIOL EPOXIDES
    Shivendra Singh; Fiscal Year: 2006
    ..In the long-term, this knowledge will guide us in developing strategies for prevention of PAH-induced cancers in humans. ..
  11. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2011
    ..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
  12. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2010
    ..In summary, the immediate and future intrinsic value of the studies proposed in this renewal application resides in optimization of DATS-based regimens for chemoprevention of prostate cancer. ..
  13. Breast Cancer Prevention by Dietary Phytochemicals
    Shivendra Singh; Fiscal Year: 2010
    ....
  14. Breast Cancer Prevention by Ayurvedic Medicine Constituents
    Shivendra Singh; Fiscal Year: 2010
    ..In summary, the hypothesis-driven and mechanistically-focused studies proposed in this application will provide preclinical data necessary for clinical development of WA as a chemopreventive agent against breast cancer in humans. ..
  15. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2010
    ..Positive outcome of these studies will justify future clinical trials to determine efficacy of PEITC for prevention of prostate cancer in humans. ..
  16. Breast Cancer Prevention by Dietary Phytochemicals
    Shivendra Singh; Fiscal Year: 2009
    ....
  17. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2009
    ..abstract_text> ..
  18. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2009
    ..In the long run, the results of the proposed studies could lead to DATS-based strategies for prevention and/or treatment of human prostate cancers. ..
  19. Prevention of Prostate Cancer by Sulforaphane
    Shivendra Singh; Fiscal Year: 2005
    ..abstract_text> ..
  20. Toxicological Relevance of Human GST P1-1 Polymorphism
    Shivendra Singh; Fiscal Year: 2005
    ..abstract_text> ..
  21. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2005
    ..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
  22. MECHANISM OF ANTICARCINOGENIC ACTIVITY--ORGANOSULFIDES
    Shivendra Singh; Fiscal Year: 2002
    ..Insights gained from these studies should help in devising practical dietary recommendation for humans. ..
  23. ROLE OF HUMAN GST PI POLYMORPHISM IN DRUG RESISTANCE
    Shivendra Singh; Fiscal Year: 2002
    ..These studies, in the long term, may be valuable in identifying subjects that may be at a higher risk for developing GST-mediated resistance to some alkylating agents. ..
  24. ROLE OF HUMAN GST PI POLYMORPHISM IN DRUG RESISTANCE
    Shivendra Singh; Fiscal Year: 2001
    ..These studies, in the long term, may be valuable in identifying subjects that may be at a higher risk for developing GST-mediated resistance to some alkylating agents. ..
  25. NOVEL GST AND DETOXICATION OF DIOL EPOXIDES
    Shivendra Singh; Fiscal Year: 2001
    ..5 and its orthologue(s) in the detoxication of these carcinogens. In the long term, strategies to manipulate this group of GSTs to the advantage of cells/organ should be extremely important for cancer prevention. ..
  26. MECHANISM OF ANTICARCINOGENIC ACTIVITY--ORGANOSULFIDES
    Shivendra Singh; Fiscal Year: 2001
    ..Insights gained from these studies should help in devising practical dietary recommendation for humans. ..
  27. TOXICOLOGICAL RELEVANCE OF HUMAN GST PL-L POLYMORPHISM
    Shivendra Singh; Fiscal Year: 2001
    ..The population will consist of members of both genders and will include Caucasians, blacks and other minorities, as available and representative of the patient base at Mercy Hospital. ..
  28. NOVEL GST AND DETOXICATION OF DIOL EPOXIDES
    Shivendra Singh; Fiscal Year: 2000
    ..5 and its orthologue(s) in the detoxication of these carcinogens. In the long term, strategies to manipulate this group of GSTs to the advantage of cells/organ should be extremely important for cancer prevention. ..
  29. TOXICOLOGICAL RELEVANCE OF HUMAN GST PL-L POLYMORPHISM
    Shivendra Singh; Fiscal Year: 2000
    ..The population will consist of members of both genders and will include Caucasians, blacks and other minorities, as available and representative of the patient base at Mercy Hospital. ..
  30. ROLE OF HUMAN GST PI POLYMORPHISM IN DRUG RESISTANCE
    Shivendra Singh; Fiscal Year: 2000
    ..These studies, in the long term, may be valuable in identifying subjects that may be at a higher risk for developing GST-mediated resistance to some alkylating agents. ..
  31. Toxicological Relevance of Human GST P1-1 Polymorphism
    Shivendra Singh; Fiscal Year: 2002
    ..abstract_text> ..
  32. NOVEL GST AND DETOXICATION OF DIOL EPOXIDES
    Shivendra Singh; Fiscal Year: 2003
    ..In the long-term, this knowledge will guide us in developing strategies for prevention of PAH-induced cancers in humans. ..
  33. Prostate Cancer Prevention by Diallyl Trisulfide
    Shivendra Singh; Fiscal Year: 2005
    ..In the long run, the results of the proposed studies could lead to DATS-based strategies for prevention and/or treatment of human prostate cancers. ..
  34. Toxicological Relevance of Human GST P1-1 Polymorphism
    Shivendra Singh; Fiscal Year: 2004
    ..abstract_text> ..
  35. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2004
    ..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
  36. Toxicological Relevance of Human GST P1-1 Polymorphism
    Shivendra Singh; Fiscal Year: 2003
    ..abstract_text> ..
  37. Anticarcinogenic Effect of ITCs Against Prostate Cancer
    Shivendra Singh; Fiscal Year: 2003
    ..Studies proposed in this application may lead to identification of an ITC analogue that can be used to delay onset and/or progression of human prostate cancer. ..
  38. MECHANISM OF ANTICARCINOGENIC ACTIVITY--ORGANOSULFIDES
    Shivendra Singh; Fiscal Year: 2003
    ..Insights gained from these studies should help in devising practical dietary recommendation for humans. ..
  39. ROLE OF HUMAN GST PI POLYMORPHISM IN DRUG RESISTANCE
    Shivendra Singh; Fiscal Year: 2003
    ..These studies, in the long term, may be valuable in identifying subjects that may be at a higher risk for developing GST-mediated resistance to some alkylating agents. ..
  40. MECHANISM OF ANTICARCINOGENIC ACTIVITY--ORGANOSULFIDES
    Shivendra Singh; Fiscal Year: 2000
    ..Insights gained from these studies should help in devising practical dietary recommendation for humans. ..