Research Topics
| N P SinghSummaryAffiliation: University of Washington Country: USA Publications
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Publications
Cytotoxicity of ethanolic extracts of Artemisia annua to Molt-4 human leukemia cellsNarendra P Singh
Department of Bioengineering, University of Washington, Seattle, WA, USA
Planta Med 77:1788-93. 2011..However, at 48 and 72 hr, the toxicity in leukocytes for any of the treatment groups was not significantly different. These experiments suggest that these extracts may have potential application in cancer treatment...
Case report of a pituitary macroadenoma treated with artemetherNarendra P Singh
Department of Bioengineering, Box 355061, University of Washington, Seattle, WA 98195, USA
Integr Cancer Ther 5:391-4. 2006..This report describes the case of a male patient with pituitary macroadenoma treated with the artemisinin analog artemether...- Synergistic cytotoxicity of artemisinin and sodium butyrate on human cancer cellsNarendra P Singh
Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
Anticancer Res 25:4325-31. 2005..In the present study, the efficacy of a combined treatment of DHA and butyric acid at low doses in killing cancer cells was investigated... - Artemisinin induces apoptosis in human cancer cellsNarendra P Singh
Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
Anticancer Res 24:2277-80. 2004..It has been shown to selectively kill cancer cells in vitro and retard the growth of implanted fibrosarcoma tumors in rats. In the present research, we investigated its mechanism of cytotoxicity to cancer cells... - Effects of age on DNA double-strand breaks and apoptosis in human spermNarendra P Singh
Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
Fertil Steril 80:1420-30. 2003..This study was designed to explore the relationship between men's age and DNA damage and apoptosis in human spermatozoa... - White light-mediated DNA strand breaks in lens epithelial cellsNarendra P Singh
Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
Exp Eye Res 75:555-60. 2002..Results of these experiments confirm our conclusion that visible light induces DNA damage in cells. These findings may be relevant in a genotoxicity study on cells ex vivo... - Microgel electrophoresis: sensitivity, mechanisms, and DNA electrostretchingN P Singh
Bioelectromagnetics Research Laboratory, University of Washington, Seattle 98195 7962, USA
Mutat Res 383:167-75. 1997..We capitalize on this behavior by developing a neutral microgel method to stretch chromosomes... - DNA double-strand breaks in mouse kidney cells with ageN P Singh
Department of Bioengineering, University of Washington, Seattle 98195, USA
Biogerontology 2:261-70. 2001..1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys... - Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cellsN P Singh
Department of Bioengineering, University of Washington, Seattle 98195 7962, USA
Life Sci 70:49-56. 2001..Since it is relatively easy to increase the iron content inside cancer cells in vivo, administration of artemisinin-like drugs and intracellular iron-enhancing compounds may be a simple, effective, and economical treatment for cancer... - A rapid method for the preparation of single-cell suspensions from solid tissuesN P Singh
Department of Bioengineering, University of Washington, Seattle 98195 7962, USA
Cytometry 31:229-32. 1998..The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described... - Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosisN P Singh
Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
Mutat Res 455:111-27. 2000..Some experiments based on each methodology are included here. A new protocol for rapid and efficient precipitation of DNA in microgel is included. A step by step description of laboratory protocol is also included... - A simple method for accurate estimation of apoptotic cellsN P Singh
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
Exp Cell Res 256:328-37. 2000..Validation of the technique was done using some known inducers of apoptosis and necrosis (hyperthermia, hydrogen peroxide, mitoxantrone, novobiocin, and sodium ascorbate)... - Visual quantification of DNA double-strand breaks in bacteriaN P Singh
Department of Bioengineering, University of Washington, Bioelectromagnetics Research Laboratory, Box 357962, Seattle, WA, USA
Mutat Res 429:159-68. 1999..5 rad and an increase in the number of DNA breaks induced by a dose as low as 25 rad. The extent of DNA migration and number of breaks were directly correlated to X-ray dosage... - 60 Hz magnetic field exposure induces DNA crosslinks in rat brain cellsN Singh
Bioelectromagnetics Research Laboratory, Department of Bioengineering, University of Washington, Seattle, WA, USA
Mutat Res 400:313-20. 1998..The pattern of effects is similar between the two agents. These data suggest that both DNA-protein and DNA-DNA crosslinks are formed in brain cells of rats after acute exposure to a 60 Hz magnetic field... - X-ray induced DNA double-strand breaks in human spermN P Singh
Department of Bioengineering, School of Medicine, University of Washington, Seattle 98195, USA
Mutagenesis 13:75-9. 1998..An increase in DNA migration was observed with as low as 12.5 cGy, but damage was observed in all samples at 25 cGy. No evidence of repair of these X-ray-induced DNA double-strand breaks was observed during a 2 h period... - Melatonin and N-tert-butyl-alpha-phenylnitrone block 60-Hz magnetic field-induced DNA single and double strand breaks in rat brain cellsH Lai
Bioelectromagnetics Research Laboratory, Center for Bioengineering, University of Washington, Seattle 98195, USA
J Pineal Res 22:152-62. 1997..Since melatonin and PBN are efficient free radical scavengers, these data suggest that free radicals may play a role in magnetic field-induced DNA damage... - Lack of an association between environmental exposure to polychlorinated biphenyls and p,p'-DDE and DNA damage in human sperm measured using the neutral comet assayR Hauser
Department of Environmental Health, Occupational Health Program, Harvard School of Public Health, Boston, MA 02115, USA
Hum Reprod 18:2525-33. 2003.... - DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolitesR Hauser
Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA
Hum Reprod 22:688-95. 2007.... - Influence of p53 zygosity on select sperm parameters of the mouseM S Marty
Health and Environmental Research Laboratories, The Dow Chemical Company, 1803 Building, Midland, MI 48674, USA
Mutat Res 427:39-45. 1999.... - Reliability of the comet assay in cryopreserved human spermS M Duty
Environmental Health Department, Occupational Health Program, Harvard School of Public Health, Boston, MA 02115-9957, USA
Hum Reprod 17:1274-80. 2002.... - An uncommon cause of sclerodermaS Sikdar
Department of Medicine, Maulana Azad Medical College and Associated Hospitals, New Delhi, India
Scand J Rheumatol 34:242-5. 2005..To the best of our knowledge, the association of HIV with scleroderma has not previously been reported... - Stroke: a rare presentation of cardiac hydatidosisN P Singh
Department of Medicine, Maulana Azad Medical College and Lok Navak Hospital, MR Centre, New Delhi, India
Neurol India 51:120-1. 2003..Echocardiography showed a hydatid cyst in the right atrial chamber extending into the left atrium. A single hepatic hydatid was also seen... - Staphylococcal pericarditis in a chronic renal failure patientN P Singh
Department of Medicine, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi, India
Ren Fail 25:493-8. 2003..After toxemia improved, paradox decreased and fever subsided, the pericardial catheter was removed and antibiotics continued for a period of four weeks. Maintenance hemodialysis was continued during hospital stay and after discharge... - Mediastinitis presenting as pyrexia of unknown originA Prakash
Department of Medicine, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi-110 002
J Assoc Physicians India 51:405-7. 2003..Patient responded to parenteral antibiotics (ceftriaxone and metronidazole) and hydrocortisone with complete resolution in 10 days... - Chromatin structure in globozoospermia: a case reportK L Larson
Department of Chemistry and Biochemistry, South Dakota State University, Brookings, USA
J Androl 22:424-31. 2001..Lower success rates seen in some patients may be due to unrelated factors... - A clinico-mycological study of dermatophytoses in NepalA Agarwalla
Department of Dermatology and Venereology, B.P. Koirala Institute of Health Sciences, Dharan, Napal
J Dermatol 28:16-21. 2001..rubrum (45.74%) as the most common species followed by T. mentagrophytes (26.6%), T. tonsurans (11.7%), M. audouinii (8.36%), E. floccosum (4.26%), T. schoenlenii (2.13%), T. violaceum (2.13%) and T. verrucosum (1.06%)...