N P Singh

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. ncbi request reprint A simple method for accurate estimation of apoptotic cells
    N P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Exp Cell Res 256:328-37. 2000
  2. ncbi request reprint Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis
    N P Singh
    Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
    Mutat Res 455:111-27. 2000
  3. doi request reprint Cytotoxicity of ethanolic extracts of Artemisia annua to Molt-4 human leukemia cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Planta Med 77:1788-93. 2011
  4. ncbi request reprint Case report of a pituitary macroadenoma treated with artemether
    Narendra P Singh
    Department of Bioengineering, Box 355061, University of Washington, Seattle, WA 98195, USA
    Integr Cancer Ther 5:391-4. 2006
  5. ncbi request reprint Synergistic cytotoxicity of artemisinin and sodium butyrate on human cancer cells
    Narendra P Singh
    Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
    Anticancer Res 25:4325-31. 2005
  6. ncbi request reprint Artemisinin induces apoptosis in human cancer cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
    Anticancer Res 24:2277-80. 2004
  7. ncbi request reprint Effects of age on DNA double-strand breaks and apoptosis in human sperm
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
    Fertil Steril 80:1420-30. 2003
  8. ncbi request reprint White light-mediated DNA strand breaks in lens epithelial cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Exp Eye Res 75:555-60. 2002
  9. ncbi request reprint Microgel electrophoresis: sensitivity, mechanisms, and DNA electrostretching
    N P Singh
    Bioelectromagnetics Research Laboratory, University of Washington, Seattle 98195 7962, USA
    Mutat Res 383:167-75. 1997
  10. ncbi request reprint DNA double-strand breaks in mouse kidney cells with age
    N P Singh
    Department of Bioengineering, University of Washington, Seattle 98195, USA
    Biogerontology 2:261-70. 2001

Collaborators

Detail Information

Publications26

  1. ncbi request reprint A simple method for accurate estimation of apoptotic cells
    N P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
    Exp Cell Res 256:328-37. 2000
    ..Validation of the technique was done using some known inducers of apoptosis and necrosis (hyperthermia, hydrogen peroxide, mitoxantrone, novobiocin, and sodium ascorbate)...
  2. ncbi request reprint Microgels for estimation of DNA strand breaks, DNA protein crosslinks and apoptosis
    N P Singh
    Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
    Mutat Res 455:111-27. 2000
    ..Some experiments based on each methodology are included here. A new protocol for rapid and efficient precipitation of DNA in microgel is included. A step by step description of laboratory protocol is also included...
  3. doi request reprint Cytotoxicity of ethanolic extracts of Artemisia annua to Molt-4 human leukemia cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, WA, USA
    Planta Med 77:1788-93. 2011
    ..However, at 48 and 72 hr, the toxicity in leukocytes for any of the treatment groups was not significantly different. These experiments suggest that these extracts may have potential application in cancer treatment...
  4. ncbi request reprint Case report of a pituitary macroadenoma treated with artemether
    Narendra P Singh
    Department of Bioengineering, Box 355061, University of Washington, Seattle, WA 98195, USA
    Integr Cancer Ther 5:391-4. 2006
    ..This report describes the case of a male patient with pituitary macroadenoma treated with the artemisinin analog artemether...
  5. ncbi request reprint Synergistic cytotoxicity of artemisinin and sodium butyrate on human cancer cells
    Narendra P Singh
    Department of Bioengineering, Box 357962, University of Washington, Seattle, WA 98195 7962, USA
    Anticancer Res 25:4325-31. 2005
    ..In the present study, the efficacy of a combined treatment of DHA and butyric acid at low doses in killing cancer cells was investigated...
  6. ncbi request reprint Artemisinin induces apoptosis in human cancer cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
    Anticancer Res 24:2277-80. 2004
    ..It has been shown to selectively kill cancer cells in vitro and retard the growth of implanted fibrosarcoma tumors in rats. In the present research, we investigated its mechanism of cytotoxicity to cancer cells...
  7. ncbi request reprint Effects of age on DNA double-strand breaks and apoptosis in human sperm
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, Washington 98195 7962, USA
    Fertil Steril 80:1420-30. 2003
    ..This study was designed to explore the relationship between men's age and DNA damage and apoptosis in human spermatozoa...
  8. ncbi request reprint White light-mediated DNA strand breaks in lens epithelial cells
    Narendra P Singh
    Department of Bioengineering, University of Washington, Seattle, WA 98195, USA
    Exp Eye Res 75:555-60. 2002
    ..Results of these experiments confirm our conclusion that visible light induces DNA damage in cells. These findings may be relevant in a genotoxicity study on cells ex vivo...
  9. ncbi request reprint Microgel electrophoresis: sensitivity, mechanisms, and DNA electrostretching
    N P Singh
    Bioelectromagnetics Research Laboratory, University of Washington, Seattle 98195 7962, USA
    Mutat Res 383:167-75. 1997
    ..We capitalize on this behavior by developing a neutral microgel method to stretch chromosomes...
  10. ncbi request reprint DNA double-strand breaks in mouse kidney cells with age
    N P Singh
    Department of Bioengineering, University of Washington, Seattle 98195, USA
    Biogerontology 2:261-70. 2001
    ..1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys...
  11. ncbi request reprint Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells
    N P Singh
    Department of Bioengineering, University of Washington, Seattle 98195 7962, USA
    Life Sci 70:49-56. 2001
    ..Since it is relatively easy to increase the iron content inside cancer cells in vivo, administration of artemisinin-like drugs and intracellular iron-enhancing compounds may be a simple, effective, and economical treatment for cancer...
  12. ncbi request reprint A rapid method for the preparation of single-cell suspensions from solid tissues
    N P Singh
    Department of Bioengineering, University of Washington, Seattle 98195 7962, USA
    Cytometry 31:229-32. 1998
    ..The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described...
  13. ncbi request reprint Visual quantification of DNA double-strand breaks in bacteria
    N P Singh
    Department of Bioengineering, University of Washington, Bioelectromagnetics Research Laboratory, Box 357962, Seattle, WA, USA
    Mutat Res 429:159-68. 1999
    ..5 rad and an increase in the number of DNA breaks induced by a dose as low as 25 rad. The extent of DNA migration and number of breaks were directly correlated to X-ray dosage...
  14. ncbi request reprint 60 Hz magnetic field exposure induces DNA crosslinks in rat brain cells
    N Singh
    Bioelectromagnetics Research Laboratory, Department of Bioengineering, University of Washington, Seattle, WA, USA
    Mutat Res 400:313-20. 1998
    ..The pattern of effects is similar between the two agents. These data suggest that both DNA-protein and DNA-DNA crosslinks are formed in brain cells of rats after acute exposure to a 60 Hz magnetic field...
  15. ncbi request reprint X-ray induced DNA double-strand breaks in human sperm
    N P Singh
    Department of Bioengineering, School of Medicine, University of Washington, Seattle 98195, USA
    Mutagenesis 13:75-9. 1998
    ..An increase in DNA migration was observed with as low as 12.5 cGy, but damage was observed in all samples at 25 cGy. No evidence of repair of these X-ray-induced DNA double-strand breaks was observed during a 2 h period...
  16. ncbi request reprint Melatonin and N-tert-butyl-alpha-phenylnitrone block 60-Hz magnetic field-induced DNA single and double strand breaks in rat brain cells
    H Lai
    Bioelectromagnetics Research Laboratory, Center for Bioengineering, University of Washington, Seattle 98195, USA
    J Pineal Res 22:152-62. 1997
    ..Since melatonin and PBN are efficient free radical scavengers, these data suggest that free radicals may play a role in magnetic field-induced DNA damage...
  17. ncbi request reprint Lack of an association between environmental exposure to polychlorinated biphenyls and p,p'-DDE and DNA damage in human sperm measured using the neutral comet assay
    R Hauser
    Department of Environmental Health, Occupational Health Program, Harvard School of Public Health, Boston, MA 02115, USA
    Hum Reprod 18:2525-33. 2003
    ....
  18. ncbi request reprint DNA damage in human sperm is related to urinary levels of phthalate monoester and oxidative metabolites
    R Hauser
    Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA
    Hum Reprod 22:688-95. 2007
    ....
  19. ncbi request reprint Influence of p53 zygosity on select sperm parameters of the mouse
    M S Marty
    Health and Environmental Research Laboratories, The Dow Chemical Company, 1803 Building, Midland, MI 48674, USA
    Mutat Res 427:39-45. 1999
    ....
  20. ncbi request reprint Reliability of the comet assay in cryopreserved human sperm
    S M Duty
    Environmental Health Department, Occupational Health Program, Harvard School of Public Health, Boston, MA 02115 9957, USA
    Hum Reprod 17:1274-80. 2002
    ..Therefore, before its use in epidemiological studies, the reliability of the comet assay in measuring DNA damage in cryopreserved sperm should be compared with that in fresh human sperm...
  21. ncbi request reprint An uncommon cause of scleroderma
    S Sikdar
    Department of Medicine, Maulana Azad Medical College and Associated Hospitals, New Delhi, India
    Scand J Rheumatol 34:242-5. 2005
    ..To the best of our knowledge, the association of HIV with scleroderma has not previously been reported...
  22. ncbi request reprint Stroke: a rare presentation of cardiac hydatidosis
    N P Singh
    Department of Medicine, Maulana Azad Medical College and Lok Navak Hospital, MR Centre, New Delhi, India
    Neurol India 51:120-1. 2003
    ..Echocardiography showed a hydatid cyst in the right atrial chamber extending into the left atrium. A single hepatic hydatid was also seen...
  23. ncbi request reprint Staphylococcal pericarditis in a chronic renal failure patient
    N P Singh
    Department of Medicine, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi, India
    Ren Fail 25:493-8. 2003
    ..After toxemia improved, paradox decreased and fever subsided, the pericardial catheter was removed and antibiotics continued for a period of four weeks. Maintenance hemodialysis was continued during hospital stay and after discharge...
  24. ncbi request reprint Mediastinitis presenting as pyrexia of unknown origin
    A Prakash
    Department of Medicine, Maulana Azad Medical College and Lok Nayak Hospital, New Delhi 110 002
    J Assoc Physicians India 51:405-7. 2003
    ..Patient responded to parenteral antibiotics (ceftriaxone and metronidazole) and hydrocortisone with complete resolution in 10 days...
  25. ncbi request reprint Chromatin structure in globozoospermia: a case report
    K L Larson
    Department of Chemistry and Biochemistry, South Dakota State University, Brookings, USA
    J Androl 22:424-31. 2001
    ..Lower success rates seen in some patients may be due to unrelated factors...
  26. ncbi request reprint A clinico-mycological study of dermatophytoses in Nepal
    A Agarwalla
    Department of Dermatology and Venereology, B.P. Koirala Institute of Health Sciences, Dharan, Napal
    J Dermatol 28:16-21. 2001
    ..rubrum (45.74%) as the most common species followed by T. mentagrophytes (26.6%), T. tonsurans (11.7%), M. audouinii (8.36%), E. floccosum (4.26%), T. schoenlenii (2.13%), T. violaceum (2.13%) and T. verrucosum (1.06%)...