Ishwar S Singh

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. doi request reprint Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis
    Tapas K Makar
    VA Maryland Healthcare System, Baltimore, MD 21201, United States
    J Neuroimmunol 196:67-81. 2008
  2. doi request reprint Fever, hyperthermia and the heat shock response
    Ishwar S Singh
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA
    Int J Hyperthermia 29:423-35. 2013
  3. ncbi request reprint A high affinity HSF-1 binding site in the 5'-untranslated region of the murine tumor necrosis factor-alpha gene is a transcriptional repressor
    Ishwar S Singh
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 277:4981-8. 2002
  4. pmc Febrile-range temperature modifies cytokine gene expression in LPS-stimulated macrophages by differentially modifying NF-{kappa}B recruitment to cytokine gene promoters
    Zachary A Cooper
    Univ of Maryland School of Medicine, Health Science Facility II, Rm S311, 20 Penn St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 298:C171-81. 2010
  5. pmc Toll-like receptor agonists and febrile range hyperthermia synergize to induce heat shock protein 70 expression and extracellular release
    Aditi Gupta
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 288:2756-66. 2013
  6. pmc Hyperthermia promotes and prevents respiratory epithelial apoptosis through distinct mechanisms
    Ashish Nagarsekar
    Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Respir Cell Mol Biol 47:824-33. 2012
  7. ncbi request reprint Bacterial endotoxin modifies heat shock factor-1 activity in RAW 264.7 cells: implications for TNF-alpha regulation during exposure to febrile range temperatures
    Ishwar S Singh
    Department of Medicine, University of Maryland and Medical and Research Services of the Baltimore VA Medical Center, Baltimore, Maryland 21201, USA
    J Endotoxin Res 10:175-84. 2004
  8. ncbi request reprint Inhibitor-kappaB kinase-beta regulates LPS-induced TNF-alpha production in cardiac myocytes through modulation of NF-kappaB p65 subunit phosphorylation
    Gentzon Hall
    Dept of Biochemistry and Molecular Biology, Univ of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 289:H2103-11. 2005
  9. pmc Distinct, gene-specific effect of heat shock on heat shock factor-1 recruitment and gene expression of CXC chemokine genes
    Tapan K Maity
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, USA
    Cytokine 54:61-7. 2011
  10. pmc Heat shock co-activates interleukin-8 transcription
    Ishwar S Singh
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Am J Respir Cell Mol Biol 39:235-42. 2008

Collaborators

Detail Information

Publications34

  1. doi request reprint Stem cell based delivery of IFN-beta reduces relapses in experimental autoimmune encephalomyelitis
    Tapas K Makar
    VA Maryland Healthcare System, Baltimore, MD 21201, United States
    J Neuroimmunol 196:67-81. 2008
    ..These results suggest that BMSCs can be used as vehicles to deliver the IFN-beta into the CNS. This is a potentially novel therapeutic approach which might be used in MS and other diseases of the CNS in which drug access is limited...
  2. doi request reprint Fever, hyperthermia and the heat shock response
    Ishwar S Singh
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA
    Int J Hyperthermia 29:423-35. 2013
    ....
  3. ncbi request reprint A high affinity HSF-1 binding site in the 5'-untranslated region of the murine tumor necrosis factor-alpha gene is a transcriptional repressor
    Ishwar S Singh
    Department of Medicine, Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 277:4981-8. 2002
    ..5 degrees C. This suggests that HSF-1 might repress TNFalpha transcription through redundant mechanisms, some of which might not require high affinity binding of HSF-1...
  4. pmc Febrile-range temperature modifies cytokine gene expression in LPS-stimulated macrophages by differentially modifying NF-{kappa}B recruitment to cytokine gene promoters
    Zachary A Cooper
    Univ of Maryland School of Medicine, Health Science Facility II, Rm S311, 20 Penn St, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 298:C171-81. 2010
    ..These data suggest that FRT exerts its effects on cytokine gene expression in a gene-specific manner through distal effects on promoter activation rather than proximal receptor activation and signal transduction...
  5. pmc Toll-like receptor agonists and febrile range hyperthermia synergize to induce heat shock protein 70 expression and extracellular release
    Aditi Gupta
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    J Biol Chem 288:2756-66. 2013
    ..We propose a model of how enhanced Hsp70 expression and extracellular release in patients concurrently exposed to fever and TLR agonists may contribute to the pathogenesis of sepsis...
  6. pmc Hyperthermia promotes and prevents respiratory epithelial apoptosis through distinct mechanisms
    Ashish Nagarsekar
    Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Am J Respir Cell Mol Biol 47:824-33. 2012
    ..Thus, depending on the temperature and timing relative to death receptor activation, hyperthermia can exert pro- and antiapoptotic effects through distinct mechanisms...
  7. ncbi request reprint Bacterial endotoxin modifies heat shock factor-1 activity in RAW 264.7 cells: implications for TNF-alpha regulation during exposure to febrile range temperatures
    Ishwar S Singh
    Department of Medicine, University of Maryland and Medical and Research Services of the Baltimore VA Medical Center, Baltimore, Maryland 21201, USA
    J Endotoxin Res 10:175-84. 2004
    ....
  8. ncbi request reprint Inhibitor-kappaB kinase-beta regulates LPS-induced TNF-alpha production in cardiac myocytes through modulation of NF-kappaB p65 subunit phosphorylation
    Gentzon Hall
    Dept of Biochemistry and Molecular Biology, Univ of Maryland School of Medicine, 108 N Greene St, Baltimore, MD 21201, USA
    Am J Physiol Heart Circ Physiol 289:H2103-11. 2005
    ....
  9. pmc Distinct, gene-specific effect of heat shock on heat shock factor-1 recruitment and gene expression of CXC chemokine genes
    Tapan K Maity
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, USA
    Cytokine 54:61-7. 2011
    ..We speculate, the physical proximity and interactions of other transcription factors and co-regulators with HSF1 could be critical to determining the effects of HS on the expression of these genes...
  10. pmc Heat shock co-activates interleukin-8 transcription
    Ishwar S Singh
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Am J Respir Cell Mol Biol 39:235-42. 2008
    ..We speculate that during evolution the CXC chemokine genes may have co-opted elements of the HS response to amplify their expression and enhance neutrophil delivery during febrile illnesses...
  11. doi request reprint Febrile-range hyperthermia augments reversible TNF-α-induced hyperpermeability in human microvascular lung endothelial cells
    Nirav G Shah
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Int J Hyperthermia 28:627-35. 2012
    ..A better understanding of this mechanism may provide new understanding about how fever may contribute to the pathogenesis of ALI and provide new therapeutic targets to improve clinical outcomes...
  12. pmc Febrile-range hyperthermia modifies endothelial and neutrophilic functions to promote extravasation
    Mohan E Tulapurkar
    Division of Pulmonary and Critical Care, Department of Medicine, School of Medicine, University of Maryland, 20 Penn St, Baltimore, MD 21201, USA
    Am J Respir Cell Mol Biol 46:807-14. 2012
    ....
  13. pmc Core temperature correlates with expression of selected stress and immunomodulatory genes in febrile patients with sepsis and noninfectious SIRS
    Larry A Sonna
    University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell Stress Chaperones 15:55-66. 2010
    ..For some genes, it appears that clinical condition can alter temperature-sensitive gene expression. Collectively, these data underscore the potential importance of body temperature in shaping the immune response to infection and injury...
  14. ncbi request reprint TNF-alpha increases tyrosine phosphorylation of vascular endothelial cadherin and opens the paracellular pathway through fyn activation in human lung endothelia
    Daniel J Angelini
    Division of Infectious Disease and Pulmonary, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Am J Physiol Lung Cell Mol Physiol 291:L1232-45. 2006
    ..These combined data suggest that TNF-alpha regulates the pulmonary vascular endothelial paracellular pathway, in part, through fyn activation...
  15. ncbi request reprint G-CSF, but not corticosterone, mediates circulating neutrophilia induced by febrile-range hyperthermia
    Garrettson S Ellis
    Divisions of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    J Appl Physiol (1985) 98:1799-804. 2005
    ....
  16. doi request reprint Prolonged exposure to hyperthermic stress augments neutrophil recruitment to lung during the post-exposure recovery period
    Mohan E Tulapurkar
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD21201, USA
    Int J Hyperthermia 27:717-25. 2011
    ..These previously unappreciated profound and lasting effects of long-term hyperthermia may have important consequences and may help explain the increased risk of respiratory illnesses in active duty personnel and returning veterans...
  17. ncbi request reprint CXC chemokines: a new family of heat-shock proteins?
    Ashish Nagarsekar
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine, Baltimore, Maryland, USA
    Immunol Invest 34:381-98. 2005
    ....
  18. pmc Febrile range temperature represses TNF-alpha gene expression in LPS-stimulated macrophages by selectively blocking recruitment of Sp1 to the TNF-alpha promoter
    Zachary A Cooper
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell Stress Chaperones 15:665-73. 2010
    ....
  19. doi request reprint Tolerance for chronic heat exposure is greater in female than male mice
    Tiffany M Bridges
    Pulmonary and Critical Care Medicine Division, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA
    Int J Hyperthermia 28:747-55. 2012
    ..The major objective of this study was to compare the tolerance of male and female mice for chronic heat exposure...
  20. pmc Prostaglandin E2 potentiates heat shock-induced heat shock protein 72 expression in A549 cells
    Nirav G Shah
    Division of Pulmonary and Critical Care, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Prostaglandins Other Lipid Mediat 93:1-7. 2010
    ..In summary, we showed that HS stimulates the generation of PGE(2), which augments the generation of HSPs. The clinical consequences of this pathway have yet to be determined...
  21. ncbi request reprint Hypothermia prolongs activation of NF-kappaB and augments generation of inflammatory cytokines
    Karen D Fairchild
    Department of Pediatrics, Division of Neonatology, Rm N5W68, University of Maryland Hospital, 22 S Greene Street, Baltimore, MD 21201, USA
    Am J Physiol Cell Physiol 287:C422-31. 2004
    ....
  22. pmc Hyperthermia in the febrile range induces HSP72 expression proportional to exposure temperature but not to HSF-1 DNA-binding activity in human lung epithelial A549 cells
    Mohan E Tulapurkar
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Cell Stress Chaperones 14:499-508. 2009
    ..Instead, HSF-1-mediated HSP expression following hyperthermia and heat shock appears to be mediated, in addition to HSF-1 activation, by posttranslational modifications of HSF-1 protein...
  23. pmc Febrile-range hyperthermia accelerates caspase-dependent apoptosis in human neutrophils
    Ashish Nagarsekar
    Division of Pulmonary and Critical Care Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 181:2636-43. 2008
    ..5 degrees C) doubled the proportion of bronchoalveolar PMNs undergoing apoptosis compared with euthermic mice. This process may play an important role in limiting inflammation and tissue injury during febrile illnesses...
  24. pmc Response of mice to continuous 5-day passive hyperthermia resembles human heat acclimation
    Houtan Sareh
    Division of Pulmonary and Critical Care, Department of Medicine, Baltimore VA Medical Center, Baltimore, MD 21201, USA
    Cell Stress Chaperones 16:297-307. 2011
    ..This model may be useful for analyzing the molecular mechanisms of HA and its consequences on host responsiveness to subsequent stresses...
  25. doi request reprint Brain-derived neurotrophic factor gene delivery in an animal model of multiple sclerosis using bone marrow stem cells as a vehicle
    Tapas K Makar
    VA Maryland Healthcare System, Baltimore, MD 21201, United States
    J Neuroimmunol 210:40-51. 2009
    ....
  26. pmc A central role for transcription factor C/EBP-beta in regulating CD1d gene expression in human keratinocytes
    Hashmat Sikder
    Department of Dermatology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    J Immunol 183:1657-66. 2009
    ..Together, these data show a central role for C/EBP-beta in regulating CD1d transcription...
  27. pmc Febrile-range hyperthermia augments pulmonary neutrophil recruitment and amplifies pulmonary oxygen toxicity
    Jeffrey D Hasday
    Divisions of Pulmonary and Critical Care Medicine, Department of Pathology, University of Maryland, Baltimore, Maryland 21201, USA
    Am J Pathol 162:2005-17. 2003
    ..In certain conditions, such as in the hyperoxic lung, this process may be deleterious...
  28. ncbi request reprint Endotoxin stress-response in cardiomyocytes: NF-kappaB activation and tumor necrosis factor-alpha expression
    Gary Wright
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland 21201, USA
    Am J Physiol Heart Circ Physiol 282:H872-9. 2002
    ..Taken together, these results reveal that NF-kappaB is not only involved in cytokine production but also may be linked to other pathways that subserve a constitutive, protective mechanism for the heart cell...
  29. ncbi request reprint Pulmonary and activation-regulated chemokine stimulates collagen production in lung fibroblasts
    Sergei P Atamas
    Baltimore VA Medical Center, Research Service 151, Room 3C 125, 10 North Greene Street, Baltimore, MD 21201, USA
    Am J Respir Cell Mol Biol 29:743-9. 2003
    ..Inhibition experiments with pertussis toxin suggested that PARC receptor is G protein-coupled. Thus, PARC is a member of the CC chemokine family that acts directly as a profibrotic factor...
  30. ncbi request reprint Fever, immunity, and molecular adaptations
    Jeffrey D Hasday
    Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Maryland School of Medicine and the Baltimore V A Medical Center, Baltimore, Maryland
    Compr Physiol 4:109-48. 2014
    ....
  31. ncbi request reprint Chemokine-cytokine cross-talk. The ELR+ CXC chemokine LIX (CXCL5) amplifies a proinflammatory cytokine response via a phosphatidylinositol 3-kinase-NF-kappa B pathway
    Bysani Chandrasekar
    Department of Medicine, University of Texas Health Science Center, San Antonio, Texas 78229 3900, USA
    J Biol Chem 278:4675-86. 2003
    ..Thus, in addition to attracting and activating neutrophils, the ELR(+) CXC chemokines amplify the inflammatory cascade, stimulating local production of cytokines that have negative inotropic and proapoptotic effects...
  32. ncbi request reprint Hypothermia enhances phosphorylation of I{kappa}B kinase and prolongs nuclear localization of NF-{kappa}B in lipopolysaccharide-activated macrophages
    Karen D Fairchild
    Department of Pediatrics, University of Virginia Health System, Box 800386, Charlottesville, VA 22908, USA
    Am J Physiol Cell Physiol 289:C1114-21. 2005
    ..Enhanced NF-kappaB activation may contribute to the immunomodulatory effects of HT in various clinical settings...
  33. ncbi request reprint Neutrophil elastase induces IL-8 gene transcription and protein release through p38/NF-{kappa}B activation via EGFR transactivation in a lung epithelial cell line
    Ippei Kuwahara
    Respiratory Immunology and Asthma Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA
    Am J Physiol Lung Cell Mol Physiol 291:L407-16. 2006
    ..We conclude that NE increases IL-8 transcription through p38/NF-kappaB activation via EGFR transactivation...
  34. ncbi request reprint Exercise-heat acclimation in humans alters baseline levels and ex vivo heat inducibility of HSP72 and HSP90 in peripheral blood mononuclear cells
    James P McClung
    US Army Research Institute of Environmental Medicine, Natick, MA, USA
    Am J Physiol Regul Integr Comp Physiol 294:R185-91. 2008
    ..These data demonstrate that physiological adaptations in humans undergoing HA are accompanied by both increases in baseline levels and changes in regulation of cytoprotective HSPs...

Research Grants4

  1. Differential Processing/Activation of HSF1 During Fever
    Ishwar Singh; Fiscal Year: 2007
    ..Moreover, these studies may lead to new concepts about fever management and using the heat shock/fever pathway to regulate expression of inflammatory mediators. ..
  2. Differential Processing/Activation of HSF1 During Fever
    Ishwar Singh; Fiscal Year: 2004
    ..Moreover, these studies may lead to new concepts about fever management and using the heat shock/fever pathway to regulate expression of inflammatory mediators. ..
  3. Differential Processing/Activation of HSF1 During Fever
    Ishwar Singh; Fiscal Year: 2005
    ..Moreover, these studies may lead to new concepts about fever management and using the heat shock/fever pathway to regulate expression of inflammatory mediators. ..
  4. Differential Processing/Activation of HSF1 During Fever
    Ishwar Singh; Fiscal Year: 2006
    ..Moreover, these studies may lead to new concepts about fever management and using the heat shock/fever pathway to regulate expression of inflammatory mediators. ..