Research Topics
| CHRISTOPHER SILLIMANSummaryAffiliation: University of Colorado Health Sciences Center Country: USA Publications
Research Grants
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Detail Information
Publications
The role of endothelial activation in the pathogenesis of transfusion-related acute lung injuryChristopher C Silliman
Bonfils Blood Center and the Department of Pediatrics, University of Colorado School of Medicine, Denver, Colorado 80230, USA
Transfusion 45:109S-116S. 2005- Transfusion-related acute lung injuryChristopher C Silliman
Bonfils Blood Center, University of Colorado School of Medicine, 717 Yosemite Circle, Denver, CO 80230, USA
Blood Rev 20:139-59. 2006..The definition of TRALI, patient predisposition, treatment, prevention and reporting guidelines are also examined... - Lysophosphatidylcholines prime the NADPH oxidase and stimulate multiple neutrophil functions through changes in cytosolic calciumChristopher C Silliman
Bonfils Blood Center, Denver, Colorado 80230, USA
J Leukoc Biol 73:511-24. 2003..In conclusion, lyso-PCs affect multiple PMN functions in a Ca(2)(+)-dependent manner that involves the activation of a pertussis toxin-sensitive G-protein... - Plasma and lipids from stored platelets cause acute lung injury in an animal modelChristopher C Silliman
Bonfils Blood Center, 717 Yosemite Circle, Denver, CO 80123, USA
Transfusion 43:633-40. 2003..We hypothesize that lipids generated during storage of PLTs cause TRALI in a two-event animal model... - Acute Philadelphia chromosome-positive leukemia in an adolescent boy after liver transplantationChristopher C Silliman
Bonfils Blood Center, 717 Yosemite Circle, Denver, CO 80220, U S A
J Pediatr Hematol Oncol 25:565-8. 2003..The authors conclude that this patient may have presented in a lymphoid blast crisis of chronic myelogenous leukemia with an acute T-cell leukemia/lymphoma syndrome... 
Donor antibodies to HNA-3a implicated in TRALI reactions prime neutrophils and cause PMN-mediated damage to human pulmonary microvascular endothelial cells in a two-event in vitro modelChristopher C Silliman
Bonfils Blood Center and the Department of Pediatrics, University of Colorado at Denver School of Medicine 80230, USA
Blood 109:1752-5. 2007..Thus, antibodies to HNA-3a primed PMNs and caused PMN-mediated HMVEC cytotoxicity in a two-event model identical to biologic response modifiers implicated in TRALI...- Transfusion-related acute lung injuryChristopher C Silliman
Bonfils Blood Center, 717 Yosemite St, Denver, CO 80230, USA
Blood 105:2266-73. 2005..Prevention, treatment, and a proposed definition of TRALI, especially in the context of ALI, are also examined... - Proteomic analysis of human mesenteric lymphMonika Dzieciatkowska
Department of Biochemistry and Molecular Genetics, University of Colorado, Denver, CO, USA
Shock 35:331-8. 2011..Further studies will be required to determine if the proteins identified participate in the pathogenesis of multiple organ failure and if they can be used as diagnostic markers... - Transfusion-related acute lung injury: definition and reviewPearl Toy
School of Medicine, University of California-San Francisco, San Francisco, CA 94143-0100, USA
Crit Care Med 33:721-6. 2005.... - Systemic neutrophil priming by lipid mediators in post-shock mesenteric lymph exists across speciesEric L Sarin
Departments of Surgery, Denver Health Medical Center and University of Colorado Health Sciences Center, Denver, Colorado 80204, USA
J Trauma 57:950-4. 2004..Further, the causal agent(s) exist in the lipid fraction of the lymph and are active on both human and animal PMNs... - Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activationForest R Sheppard
Department of Surgery, Denver Health Medical Center, Colorado, USA
J Leukoc Biol 78:1025-42. 2005..This review will discuss the individual components associated with the NADPH oxidase complex and the function of each of these units in each physiologic stage of the PMN: rested, primed, and activated... - The two-event model of transfusion-related acute lung injuryChristopher C Silliman
Bonfils Blood Center and Departments of Pediatrics and Surgery, University of Colorado School of Medicine, Denver, CO, USA
Crit Care Med 34:S124-31. 2006..Laboratory studies, both in vitro and in vivo, are discussed as well as human studies of TRALI. Methods to avoid patient exposure to blood components that may cause TRALI are also discussed... - Platelet-activating factor-induced clathrin-mediated endocytosis requires beta-arrestin-1 recruitment and activation of the p38 MAPK signalosome at the plasma membrane for actin bundle formationNathan J D McLaughlin
Department of Pediatrics, University of Colorado School of Medicine, Denver, CO 80262, USA
J Immunol 176:7039-50. 2006..We conclude that PAF signaling requires CME, beta-arrestin-1 recruitment of a p38 MAPK signalosome, and specific actin bundle formation at the PAFR for transduction before endosomal scission... - Soluble CD40 ligand accumulates in stored blood components, primes neutrophils through CD40, and is a potential cofactor in the development of transfusion-related acute lung injurySamina Yasmin Khan
Bonfils Blood Center, 717 Yosemite St, Denver, CO 80230, USA
Blood 108:2455-62. 2006..We concluded that sCD40L, which accumulates during blood component storage, has the capacity to activate adherent PMNs, causing endothelial damage and possibly TRALI in predisposed patients... - High-volume transfusion from male-only versus female donor plasma and hypoxemia in the critically illChristopher C Silliman
Crit Care Med 35:1775. 2007 
Arachidonic acid in postshock mesenteric lymph induces pulmonary synthesis of leukotriene B4Janeen R Jordan
Department of Surgery, University of Colorado Denver, Denver, CO, USA
J Appl Physiol 104:1161-6. 2008..Collectively, these data indicate that splanchnic ischemia/reperfusion activates gut PLA(2)-mediated release of AA into the lymph where it is delivered to the lungs, provoking LTB(4) production and subsequent PMN-mediated lung injury...- Platelet-activating factor-mediated endosome formation causes membrane translocation of p67phox and p40phox that requires recruitment and activation of p38 MAPK, Rab5a, and phosphatidylinositol 3-kinase in human neutrophilsNathan J D McLaughlin
Bonfils Blood Center, University of Colorado Denver School of Medicine, Children s Hospital, Denver, CO 80230, USA
J Immunol 180:8192-203. 2008..In conclusion, PAF transduces signals through CME, and such GPCR signaling may allow for pharmacological manipulation of these cells to decrease PMN-mediated acute organ injury... - Transfusion-induced leukocyte IL-8 gene expression is avoided by the use of human polymerized hemoglobinForest R Sheppard
Department of Surgery, University of Colorado Health Sciences Center, Denver, Colorado 80204, USA
J Trauma 57:720-4; discussion 724-5. 2004..These experimental results corroborate our previous clinical studies and further encourage the study of PolyHb as a resuscitation strategy to decrease postinjury MOF... - Acute hypoxemia in humans enhances the neutrophil inflammatory responseDouglas Y Tamura
Department of Surgery, Denver Health Medical Center, Colorado, USA
Shock 17:269-73. 2002..These results indicate that relevant acute hypoxemic events observed in the clinical setting enhance several PMN cytotoxic functions and suggest that a transient hypoxemic insult may promote hyperinflammation... - Hyperosmolarity abrogates neutrophil cytotoxicity provoked by post-shock mesenteric lymphRicardo J Gonzalez
Denver Health Medical Center, Department of Surgery, Colorado 80204, USA
Shock 18:29-32. 2002..These studies suggest inhibition of PMN signal transduction is a mechanism whereby HTS resuscitation abrogates acute lung injury... - Physiological levels of interleukin-18 stimulate multiple neutrophil functions through p38 MAP kinase activationTravis H Wyman
Bonfils Blood Center, Denver, CO 80230, USA
J Leukoc Biol 72:401-9. 2002..We conclude that IL-18, at physiologic concentrations, is an effective PMN priming agent that requires p38 MAP kinase activity... - Presence of the M-type sPLA(2) receptor on neutrophils and its role in elastase release and adhesionChristopher C Silliman
Bonfils Blood Center, Department of Pediatrics, University of Colorado School of Medicine, Denver, CO 80262, USA
Am J Physiol Cell Physiol 283:C1102-13. 2002..sPLA(2)-IB and sPLA(2)-IIA caused PMN adhesion. We conclude that PMNs contain a membrane M-type sPLA(2)-R that activates p38 MAPK... - A two-insult in vitro model of PMN-mediated pulmonary endothelial damage: requirements for adherence and chemokine releaseTravis H Wyman
Bonfils Blood Center and Department of Pediatrics, University of Colorado School of Medicine, Denver, Colorado 80230, USA
Am J Physiol Cell Physiol 283:C1592-603. 2002..In conclusion, sequential exposure of PMNs to priming agents activates the microbicidal arsenal, and PMN-mediated HMVEC damage was the result of two insults: HMVEC activation and PMN oxidase assembly... - Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factorsChristopher C Silliman
Bonfils Blood Center and the Department of Pediatrics, University of Colorado School of Medicine, Denver, USA
Blood 101:454-62. 2003..TRALI, like the acute respiratory distress syndrome, may be a 2-event phenomenon with both recipient predisposition and factors in the stored units playing major roles... - "Primed" human neutrophils on a standard peripheral blood smearorest R Sheppard
University of Colorado Health Sciences Center, Denver, USA
J Am Coll Surg 195:731. 2002 - The injured child is resistant to multiple organ failure: a different inflammatory response?Casey M Calkins
Department of Surgery, University of Colorado Health Sciences Center, Denver 80262, USA
J Trauma 53:1058-63. 2002..The purpose of this study was to describe the incidence, course, and severity of pediatric postinjury MOF. We hypothesized that the incidence and severity of postinjury MOF in children would be less when compared with adults... - Alteration of the postinjury hyperinflammatory response by means of resuscitation with a red cell substituteJeffrey L Johnson
Department of Surgery, Denver Health Medical Center, 777 Bannock Street, Denver, CO 80204, USA
J Trauma 54:133-9; discussion 139-40. 2003..Through the use of a polymerized human hemoglobin (PolyHeme), we have been able to measure the inflammatory response in patients resuscitated with minimal exposure to banked components in the early postinjury period... - Formyl-Met-Leu-Phe induces calcium-dependent tyrosine phosphorylation of Rel-1 in neutrophilsMarguerite R Kelher
Department of Surgery, University of Colorado Health Science Center School of Medicine, Denver, CO 80230, USA
Cell Calcium 34:445-55. 2003..Thus, fMLP concentrations that prime or activate the oxidase cause a rapid Ca2+-dependent tyrosine phosphorylation of Rel-1 involving p38 MAPK activation... - The abdominal compartment syndrome as a second insult during systemic neutrophil priming provokes multiple organ injuryJoao B Rezende-Neto
Department of Surgery, Faculty of Medicine of the Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil 30350-210
Shock 20:303-8. 2003..This clinically relevant two-event animal model of PMN organ injury may be useful in elucidating therapy strategies to prevent postinjury MOF... - Clinically relevant hypertonicity prevents stored blood- and lipid-mediated delayed neutrophil apoptosis independent of p38 MAPK or caspase-3 activationWalter L Biffl
Department of Surgery, Rhode Island Hospital/Brown Medical School, Providence, RI, USA
Surgery 134:86-91. 2003..HTS may activate caspase-3, but alternative signaling pathways appear to be involved in modulating the effects of lipids on PMN apoptosis... - Post-hemorrhagic shock mesenteric lymph activates human pulmonary microvascular endothelium for in vitro neutrophil-mediated injury: the role of intercellular adhesion molecule-1Ricardo J Gonzalez
Department of Surgery, Denver Health Medical Center and University of Colorado, 80204, USA
J Trauma 54:219-23. 2003..These findings further substantiate the key mechanistic role of mesenteric lymph in hemorrhagic shock-induced acute lung injury and suggest that ICAM-1 expression is pivotal in the two-event model of multiple organ failure... - Clinically relevant osmolar stress inhibits priming-induced PMN NADPH oxidase subunit translocationForest R Sheppard
Department of Surgery, Denver Health Medical Center, University of Colorado School of Medicine, Denver, Colorado, USA
J Trauma 58:752-7; discussion 757. 2005..This finding provides new insight into the mechanisms responsible for osmolar control of PMN functional responses... - Hemolytic disease of the newborn caused by a high titer anti-group B IgG from a group A motherMichael Wang
Pediatr Blood Cancer 45:861-2. 2005 - The transfusion of pre storage leukoreduced packed red blood cells to injured patientsChristopher C Silliman
Crit Care Med 36:1661-2. 2008 - Increased rate of infection associated with transfusion of old blood after severe injuryPatrick J Offner
Trauma Service, St Anthony Central Hospital, 4231 W 16th Ave, Denver, CO 80204, USA
Arch Surg 137:711-6; discussion 716-7. 2002..We hypothesized that transfusion of old blood increases infection risk in severely injured patients... - Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentratesJulie Kanter
Department of Pediatrics, Washington University, St Louis, Missouri, USA
Clin Cancer Res 14:3942-7. 2008.... - Transfusion of the injured patient: proceed with cautionChristopher C Silliman
Bonfils Blood Center, Denver Health Medical Center, Denver, Colorado 80230, USA
Shock 21:291-9. 2004..We further summarize studies with hemoglobin substitutes, whose use may obviate many of the untoward events of transfusion and promise to lead to better outcomes for injured patients...
Research Grants
- ANIMAL MODEL OF TRANSFUSION RELATED ACUTE LUNG INJURYCHRISTOPHER SILLIMAN; Fiscal Year: 2002..Completion of these specific aims will provide information to target areas for clinical intervention to make transfusions safer. ..
- Lyso-PCs activate PMNs and endothelium via PKCCHRISTOPHER SILLIMAN; Fiscal Year: 2006..Completion of these specific aims will likely result in targets for in vitro, and possibly clinical, intervention to develop methods to inhibit or to attenuate the effects of these lipids and ultimately make transfusion safer. ..