C H Sibley
Affiliation: University of Washington
- Yeast as a model system to study drugs effective against apicomplexan proteinsC H Sibley
Department of Genetics, University of Washington, Seattle, Washington, 98195 7360, USA
Methods 13:190-207. 1997..Basic protocols and methods are included. We detail some problems that might be encountered in the engineering of these yeast strains and suggest possible solutions...
- Pyrimethamine-sulfadoxine resistance in Plasmodium falciparum: what next?C H Sibley
Department of Genetics, Box 357360, University of Washington, Seattle, WA 98195 7360, USA
Trends Parasitol 17:582-8. 2001..This article reviews the molecular mechanisms of this resistance and discusses how to extend the therapeutic life of antifolate drugs...
- Mutagenesis of dihydrofolate reductase from Plasmodium falciparum: analysis in Saccharomyces cerevisiae of triple mutant alleles resistant to pyrimethamine or WR99210J T Ferlan
Department of Genetics, University of Washington, P.O. Box 357360, Seattle, WA 98195-7360, USA
Mol Biochem Parasitol 113:139-50. 2001..This collateral hypersensitivity raises the possibility that opposing selection for resistance/sensitivity to PM and WR might be used to slow selection of populations of P. falciparum resistant to antifolate treatment...
- Novel alleles of the Plasmodium falciparum dhfr highly resistant to pyrimethamine and chlorcycloguanil, but not WR99210E G Hankins
Department of Genetics, University of Washington, Seattle, WA 98195-7360, USA
Mol Biochem Parasitol 117:91-102. 2001....
- Novel approaches to tackling malarial drug resistance using yeastC H Sibley
Department of Genetics, University of Washington, Seattle, USA
IUBMB Life 52:285-9. 2001..Yeast can now be used to study antifolate drug resistance patterns that depend on the dihydrofolate reductase enzyme (DHFR) from the malaria parasite...