John Y J Shyy

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Mechanotransduction in endothelial responses to shear stress: review of work in Dr. Chien's laboratory
    J Y Shyy
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Biorheology 38:109-17. 2001
  2. ncbi request reprint Role of integrins in endothelial mechanosensing of shear stress
    John Y J Shyy
    Division of Biomedical Sciences, University of California Riverside, Riverside, Calif 92521 0121, USA
    Circ Res 91:769-75. 2002
  3. ncbi request reprint Laminar flow activates peroxisome proliferator-activated receptor-gamma in vascular endothelial cells
    Yi Liu
    Division of Biomedical Sciences, University of California at Riverside, Riverside, CA 92521 0121, USA
    Circulation 110:1128-33. 2004
  4. ncbi request reprint Regulation of endothelial cell cycle by laminar versus oscillatory flow: distinct modes of interactions of AMP-activated protein kinase and Akt pathways
    Deliang Guo
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Circ Res 100:564-71. 2007
  5. pmc Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2
    Ping Wang
    Division of Biomedical Sciences University of California Riverside, California, USA
    BMC Biochem 11:36. 2010
  6. ncbi request reprint Rho-ROCK-LIMK-cofilin pathway regulates shear stress activation of sterol regulatory element binding proteins
    Tong Lin
    Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521 0121, USA
    Circ Res 92:1296-304. 2003
  7. pmc Shear stress, SIRT1, and vascular homeostasis
    Zhen Chen
    Division of Biomedical Sciences, Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, CA 92521 0121, USA
    Proc Natl Acad Sci U S A 107:10268-73. 2010
  8. ncbi request reprint Statins activate AMP-activated protein kinase in vitro and in vivo
    Wei Sun
    Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521 0121, USA
    Circulation 114:2655-62. 2006
  9. pmc Mechanosensitive microRNAs-role in endothelial responses to shear stress and redox state
    Traci Marin
    Biomedical Sciences, School of Medicine, University of California at Riverside, Riverside, CA 92521, USA
    Free Radic Biol Med 64:61-8. 2013
  10. ncbi request reprint Vascular endothelial growth factor activation of sterol regulatory element binding protein: a potential role in angiogenesis
    Rui hai Zhou
    Division of Biomedical Sciences, University of California, Riverside Riverside 92521 0121, USA
    Circ Res 95:471-8. 2004

Collaborators

Detail Information

Publications39

  1. ncbi request reprint Mechanotransduction in endothelial responses to shear stress: review of work in Dr. Chien's laboratory
    J Y Shyy
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Biorheology 38:109-17. 2001
    ..Evidence is also provided to show that the dynamic and specific interaction between integrin and extracellular matrices is essential for mechanotransduction...
  2. ncbi request reprint Role of integrins in endothelial mechanosensing of shear stress
    John Y J Shyy
    Division of Biomedical Sciences, University of California Riverside, Riverside, Calif 92521 0121, USA
    Circ Res 91:769-75. 2002
    ..These studies show that integrins play an important role in mechanosensing in addition to their involvement in cell attachment and migration...
  3. ncbi request reprint Laminar flow activates peroxisome proliferator-activated receptor-gamma in vascular endothelial cells
    Yi Liu
    Division of Biomedical Sciences, University of California at Riverside, Riverside, CA 92521 0121, USA
    Circulation 110:1128-33. 2004
    ..We studied the activation of peroxisome proliferator-activated receptor-gamma (PPARgamma) in ECs in response to laminar flow and the associated antiinflammatory effect...
  4. ncbi request reprint Regulation of endothelial cell cycle by laminar versus oscillatory flow: distinct modes of interactions of AMP-activated protein kinase and Akt pathways
    Deliang Guo
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Circ Res 100:564-71. 2007
    ..In contrast, AMPK phosphorylation was higher in the thoracic aorta. These results provide a molecular mechanism by which laminar versus oscillatory flow regulates the endothelial cell cycle...
  5. pmc Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2
    Ping Wang
    Division of Biomedical Sciences University of California Riverside, California, USA
    BMC Biochem 11:36. 2010
    ....
  6. ncbi request reprint Rho-ROCK-LIMK-cofilin pathway regulates shear stress activation of sterol regulatory element binding proteins
    Tong Lin
    Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521 0121, USA
    Circ Res 92:1296-304. 2003
    ..By enhancing the SREBP-mediated cholesterol metabolism, this unique mechanism may contribute to endothelial cell functions under flow...
  7. pmc Shear stress, SIRT1, and vascular homeostasis
    Zhen Chen
    Division of Biomedical Sciences, Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, CA 92521 0121, USA
    Proc Natl Acad Sci U S A 107:10268-73. 2010
    ..These results suggest that atheroprotective flow, via AMPK and SIRT1, increases NO bioavailability in endothelium...
  8. ncbi request reprint Statins activate AMP-activated protein kinase in vitro and in vivo
    Wei Sun
    Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521 0121, USA
    Circulation 114:2655-62. 2006
    ..We therefore studied whether statins can activate AMPK, and if so, whether the activated AMPK regulates nitric oxide (NO) production and angiogenesis mediated by endothelial NO synthase, a substrate of AMPK in vascular endothelial cells...
  9. pmc Mechanosensitive microRNAs-role in endothelial responses to shear stress and redox state
    Traci Marin
    Biomedical Sciences, School of Medicine, University of California at Riverside, Riverside, CA 92521, USA
    Free Radic Biol Med 64:61-8. 2013
    ..Furthermore, we discuss the use of algorithms in deciphering signaling networks differentially regulated by PS and OS. ..
  10. ncbi request reprint Vascular endothelial growth factor activation of sterol regulatory element binding protein: a potential role in angiogenesis
    Rui hai Zhou
    Division of Biomedical Sciences, University of California, Riverside Riverside 92521 0121, USA
    Circ Res 95:471-8. 2004
    ..SREBP inhibition also markedly suppressed VEGF-induced angiogenesis in chick embryos. In summary, this study identifies SREBPs as the key molecules in regulating angiogenesis in response to VEGF...
  11. ncbi request reprint Stent implantation activates Akt in the vessel wall: role of mechanical stretch in vascular smooth muscle cells
    Rui hai Zhou
    Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521 0121, USA
    Arterioscler Thromb Vasc Biol 23:2015-20. 2003
    ..Multiple factors can contribute to ISR, and the underlying mechanism remains elusive. We investigated the possible role of mechanical stretch and the associated molecular signaling in ISR...
  12. pmc The antiinflammatory effect of laminar flow: the role of PPARgamma, epoxyeicosatrienoic acids, and soluble epoxide hydrolase
    Yi Liu
    Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
    Proc Natl Acad Sci U S A 102:16747-52. 2005
    ..Blocking PPARgamma by GW9662 abolished the EET/AUDA-mediated antiinflammatory effect, which indicates that PPARgamma is an effector of EETs...
  13. pmc Sterol regulatory element binding protein 2 activation of NLRP3 inflammasome in endothelium mediates hemodynamic-induced atherosclerosis susceptibility
    Han Xiao
    Division of Biomedical Sciences, University of California, Riverside, USA
    Circulation 128:632-42. 2013
    ..Here, we explored whether disturbed flow patterns activate an innate immune response to form the NLRP3 inflammasome scaffold in vascular endothelial cells via sterol regulatory element binding protein 2 (SREBP2)...
  14. pmc ATF6 modulates SREBP2-mediated lipogenesis
    Lingfang Zeng
    Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521 0121, USA
    EMBO J 23:950-8. 2004
    ..As a functional consequence, the lipogenic effect of SREBP2(N) in liver cells was suppressed by ATF6(N). Our results provide a novel mechanism by which ATF6 antagonizes SREBP2 to regulate the homeostasis of lipid and glucose...
  15. pmc AMP-activated protein kinase functionally phosphorylates endothelial nitric oxide synthase Ser633
    Zhen Chen
    Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
    Circ Res 104:496-505. 2009
    ..Nano-LC/MS/MS confirmed that eNOS purified from AICAR-treated ECs was phosphorylated at both Ser633 and Ser1177. Our results indicate that AMPK phosphorylation of eNOS Ser633 is a functional signaling event for NO bioavailability in ECs...
  16. pmc Flow-Dependent Regulation of Kruppel-Like Factor 2 Is Mediated by MicroRNA-92a
    Wei Wu
    Division of Biomedical Sciences, University of California Riverside, 92521, USA
    Circulation 124:633-41. 2011
    ..MicroRNAs (miRNAs) are noncoding small RNAs that regulate gene expression at the posttranscriptional level. We examined the role of miRNAs, particularly miR-92a, in the atheroprotective flow-regulated KLF2...
  17. ncbi request reprint Oxidative stress is involved in the development of experimental abdominal aortic aneurysm: a study of the transcription profile with complementary DNA microarray
    Noriyuki Yajima
    Division of Biomedical Sciences, University of California Riverside, CA 92521, USA
    J Vasc Surg 36:379-85. 2002
    ..We used the complementary DNA (cDNA) microarray technique to determine the transcription profile in the development of elastase-induced abdominal aortic aneurysm in rat models, with an emphasis on the oxidative stress-related genes...
  18. ncbi request reprint Differential regulation of Rho GTPases by beta1 and beta3 integrins: the role of an extracellular domain of integrin in intracellular signaling
    Hui Miao
    Department of Bioengineering and The Whitaker Institute of Biomedical Engineering, University of California, San Diego, La Jolla 92093 0427, USA
    J Cell Sci 115:2199-206. 2002
    ....
  19. ncbi request reprint The role of p53 deacetylation in p21Waf1 regulation by laminar flow
    Lingfang Zeng
    Division of Biomedical Sciences, University of California, Riverside 92521, USA
    J Biol Chem 278:24594-9. 2003
    ..The regulation of the p53-p21Waf1 pathway by laminar flow was further supported by observations that flow caused an increase of p21Waf1 level in the wild-type HCT116 (p53+/+) cells but not in the p53-null HCT116 cells...
  20. ncbi request reprint Simvastatin induces heme oxygenase-1: a novel mechanism of vessel protection
    Tzong Shyuan Lee
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Circulation 110:1296-302. 2004
    ..Here, we studied whether heme oxygenase-1 (HO-1), an important cytoprotective molecule, is induced by simvastatin and the role of HO-1 in the pleiotropic effects of simvastatin...
  21. pmc Hypoxia-responsive miRNAs target argonaute 1 to promote angiogenesis
    Zhen Chen
    Division of Biomedical Sciences, University of California, Riverside, California, USA
    J Clin Invest 123:1057-67. 2013
    ..These findings provide evidence for an angiogenic pathway involving HRMs that target AGO1 and suggest that this pathway may be a suitable target for anti- or proangiogenesis strategies...
  22. ncbi request reprint Sterol regulatory element-binding protein 1 is negatively modulated by PKA phosphorylation
    Min Lu
    Division of Biomedical Sciences, Univ of California, Riverside, Riverside, CA 92521 0121, USA
    Am J Physiol Cell Physiol 290:C1477-86. 2006
    ..Accordingly, the adenovirus-mediated expression of SREBP-1c(N)-S314D in HepG2 cells retarded lipogenesis. Our results indicate that the cAMP-PKA pathway, by phosphorylating SREBP-1, may modulate lipid metabolism in liver cell lines...
  23. pmc AMPKα2 exerts its anti-inflammatory effects through PARP-1 and Bcl-6
    Brendan Gongol
    Biochemistry and Molecular Biology Graduate Program, and Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Proc Natl Acad Sci U S A 110:3161-6. 2013
    ..Conversely, AMPKα suppression or knockdown produces the opposite effects. The results demonstrate an anti-infamatory pathway linking AMPK, PARP-1, and Bcl-6 in endothelial cells...
  24. pmc Glucagon regulates ACC activity in adipocytes through the CAMKKβ/AMPK pathway
    I Chen Peng
    Division of Biomedical Sciences, University of California, Riverside, 92521, USA
    Am J Physiol Endocrinol Metab 302:E1560-8. 2012
    ..These results indicate that CaMKKβ/AMPK signaling is an important molecular component in regulating lipid metabolism in adipocytes responding to glucagon and could be a therapeutic target for the dysregulation of energy storage...
  25. pmc Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growth
    Kuei Chun Wang
    Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA
    Proc Natl Acad Sci U S A 107:3234-9. 2010
    ..Our findings indicate that PS suppresses EC proliferation through the regulation of miR-23b and provide insights into the role of miRs in mechanotransduction...
  26. ncbi request reprint AMP-activated protein kinase is involved in endothelial NO synthase activation in response to shear stress
    Yingjia Zhang
    Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
    Arterioscler Thromb Vasc Biol 26:1281-7. 2006
    ..In this study, we investigate the regulation of the AMPK-eNOS pathway in vascular endothelial cells (ECs) by shear stress and the activation of aortic AMPK in a mouse model with a high level of voluntary running (High-Runner)...
  27. pmc Regulation of SIRT1 by oxidative stress-responsive miRNAs and a systematic approach to identify its role in the endothelium
    Zhen Chen
    1 Division of Biomedical Sciences, University of California, Riverside, California
    Antioxid Redox Signal 19:1522-38. 2013
    ..Accumulated evidence suggests that sirtuin 1 (SIRT1) expression and/or activity is impaired by supraphysiological levels of oxidative stress, which in turn disrupts endothelial homeostasis...
  28. pmc Intervertebral disc degeneration: the role of the mitochondrial pathway in annulus fibrosus cell apoptosis induced by overload
    Francois Rannou
    Division of Biomedical Sciences, University of California, Riverside, California 92521 0121, USA
    Am J Pathol 164:915-24. 2004
    ..Our results from human samples, mouse models, and annulus fibrosus culture experiments demonstrate that the mechanical overload-induced IVD degeneration is mediated through the mitochondrial apoptotic pathway in IVD cells...
  29. doi request reprint microRNA: a master regulator of cellular processes for bioengineering systems
    Wei Sun
    Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
    Annu Rev Biomed Eng 12:1-27. 2010
    ....
  30. ncbi request reprint Sterol-responsive element-binding protein (SREBP) 2 down-regulates ATP-binding cassette transporter A1 in vascular endothelial cells: a novel role of SREBP in regulating cholesterol metabolism
    Lingfang Zeng
    Division of Biomedical Sciences, University of California, Riverside, Riverside, California 92521, USA
    J Biol Chem 279:48801-7. 2004
    ..Thus, this SREBP-regulated mechanism may control the efflux of cholesterol, which is a newly defined function of SREBP2 in ECs in addition to its role in cholesterol uptake and biosynthesis...
  31. doi request reprint Fluorescence proteins, live-cell imaging, and mechanobiology: seeing is believing
    Yingxiao Wang
    Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
    Annu Rev Biomed Eng 10:1-38. 2008
    ..These live-cell imaging technologies, which represent a frontier area in biomedical engineering, can shed new light on the mechanisms regulating mechanobiology at cellular and molecular levels in normal and pathophysiological conditions...
  32. ncbi request reprint Shear stress activation of SREBP1 in endothelial cells is mediated by integrins
    Yi Liu
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, USA
    Arterioscler Thromb Vasc Biol 22:76-81. 2002
    ..These results demonstrate that integrin signaling plays a critical role in the modulation of SREBP in ECs in response to shear stress...
  33. ncbi request reprint Role for sterol regulatory element-binding protein in activation of endothelial cells by phospholipid oxidation products
    Michael Yeh
    Department of Medicine, Geffen School of Medicine at University of California Los Angeles, USA
    Circ Res 95:780-8. 2004
    ..The current studies suggest a novel role for endothelial cholesterol depletion and subsequent SREBP activation in inflammatory processes in which phospholipid oxidation products accumulate...
  34. ncbi request reprint Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting liver X receptor (LXR)
    Yi Zhu
    Department of Physiology, Key Laboratory of Molecular Cardiovascular Sciences of Education Ministry, Health Science Center, Peking University, Beijing 100083, China
    Cardiovasc Res 68:425-32. 2005
    ..This report describes the effect of copper-catalyzed oxLDL on the regulation of ABCA1 in human endothelial cells (ECs)...
  35. ncbi request reprint Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathway
    Yingxiao Wang
    Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla 92093 0427, USA
    Am J Physiol Heart Circ Physiol 286:H685-92. 2004
    ..Therefore, our results indicate that the signaling events induced by shear stress and VEGF converge at the membrane receptor Flk-1 and that these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation...
  36. ncbi request reprint Cholesterol loading increases the translocation of ATP synthase beta chain into membrane caveolae in vascular endothelial cells
    Tengke Wang
    Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100083, China
    Biochim Biophys Acta 1761:1182-90. 2006
    ..Thus, the ectopic localization of ATPS-beta may participate in the energy balance of cells in response to the change in intracellular cholesterol levels...
  37. ncbi request reprint Interplay between integrins and FLK-1 in shear stress-induced signaling
    Yingxiao Wang
    Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, California 92093, USA
    Am J Physiol Cell Physiol 283:C1540-7. 2002
    ..Integrins are essential for the mechanical activation of Flk-1 by shear stress but not for the chemical activation of Flk-1 by VEGF...
  38. ncbi request reprint Laminar shear stress regulates liver X receptor in vascular endothelial cells
    Minjia Zhu
    Department of Physiology and Pathophysiology, Peking University, Health Sciences Center, Beijing, China 100083
    Arterioscler Thromb Vasc Biol 28:527-33. 2008
    ..The liver X receptors (LXRs) regulate a set of genes involved in lipid metabolism and reverse cholesterol transport. We investigated the mechanism by which shear stress regulates LXR in vascular endothelial cells (ECs)...
  39. pmc Visualization of polarized membrane type 1 matrix metalloproteinase activity in live cells by fluorescence resonance energy transfer imaging
    Mingxing Ouyang
    Department of Bioengineering and the Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Illinois 61801, USA
    J Biol Chem 283:17740-8. 2008
    ..Hence, the MT1-MMP biosensor provides a powerful tool for characterizing the molecular processes underlying the spatiotemporal regulation of this critical class of enzymes...

Research Grants16

  1. Shear stress, SIRT1, and Arterial Stiffening
    John Y J Shyy; Fiscal Year: 2010
    ..Results from the proposed experiments are likely to contribute to novel therapies for its prevention and/or treatment. ..
  2. Shear Stress, AMPK, and Endothelial Functions
    John Shyy; Fiscal Year: 2009
    ..The proposal will significantly increase our understanding of mechanisms by which blood flow patterns interplay with hyperlipidemia in atherogenesis, which are likely to contribute to novel therapies for its prevention and/or treatment. ..
  3. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 2002
    ....
  4. Flow Regulation of SREBP in Atherosclerosis
    John Shyy; Fiscal Year: 2007
    ..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
  5. Shear Stress, AMPK, and Endothelial Functions
    John Y J Shyy; Fiscal Year: 2010
    ....
  6. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 1999
    ....
  7. Shear Stress, AMPK, and Endothelial Functions
    John Shyy; Fiscal Year: 2009
    ....
  8. Flow Regulation of SREBP in Atherosclerosis
    John Shyy; Fiscal Year: 2006
    ..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
  9. Flow Regulation of SREBP in Atherosclerosis
    John Shyy; Fiscal Year: 2005
    ..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
  10. Flow Regulation of SREBP in Atherosclerosis
    John Shyy; Fiscal Year: 2004
    ..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
  11. SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATION
    John Shyy; Fiscal Year: 2003
    ....
  12. SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATION
    John Shyy; Fiscal Year: 2002
    ....
  13. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 2001
    ....
  14. SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATION
    John Shyy; Fiscal Year: 2001
    ....
  15. SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATION
    John Shyy; Fiscal Year: 2000
    ....
  16. STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSIS
    John Shyy; Fiscal Year: 2000
    ....