Research Topics
Genomes and Genes
| John Y J ShyySummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Mechanotransduction in endothelial responses to shear stress: review of work in Dr. Chien's laboratoryJ Y Shyy
Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
Biorheology 38:109-17. 2001..Evidence is also provided to show that the dynamic and specific interaction between integrin and extracellular matrices is essential for mechanotransduction...- Role of integrins in endothelial mechanosensing of shear stressJohn Y J Shyy
Division of Biomedical Sciences, University of California Riverside, Riverside, Calif 92521 0121, USA
Circ Res 91:769-75. 2002..These studies show that integrins play an important role in mechanosensing in addition to their involvement in cell attachment and migration... - Laminar flow activates peroxisome proliferator-activated receptor-gamma in vascular endothelial cellsYi Liu
Division of Biomedical Sciences, University of California at Riverside, Riverside, CA 92521-0121, USA
Circulation 110:1128-33. 2004..The flow production of PPARgamma ligands is through the PLA2-CYP450 pathway, and the induced PPARgamma ligands exert antiinflammatory effects in several types of cells... - Regulation of endothelial cell cycle by laminar versus oscillatory flow: distinct modes of interactions of AMP-activated protein kinase and Akt pathwaysDeliang Guo
Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
Circ Res 100:564-71. 2007..In contrast, AMPK phosphorylation was higher in the thoracic aorta. These results provide a molecular mechanism by which laminar versus oscillatory flow regulates the endothelial cell cycle... 
Beta-arrestin inhibits CAMKKbeta-dependent AMPK activation downstream of protease-activated-receptor-2Ping Wang
Division of Biomedical Sciences University of California Riverside, California, USA
BMC Biochem 11:36. 2010....- Shear stress, SIRT1, and vascular homeostasisZhen Chen
Division of Biomedical Sciences, Biochemistry and Molecular Biology Graduate Program, University of California, Riverside, CA 92521 0121, USA
Proc Natl Acad Sci U S A 107:10268-73. 2010..These results suggest that atheroprotective flow, via AMPK and SIRT1, increases NO bioavailability in endothelium... - Simvastatin induces heme oxygenase-1: a novel mechanism of vessel protectionTzong Shyuan Lee
Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
Circulation 110:1296-302. 2004..Here, we studied whether heme oxygenase-1 (HO-1), an important cytoprotective molecule, is induced by simvastatin and the role of HO-1 in the pleiotropic effects of simvastatin... - ATF6 modulates SREBP2-mediated lipogenesisLingfang Zeng
Division of Biomedical Sciences, University of California, Riverside, Riverside, CA 92521 0121, USA
EMBO J 23:950-8. 2004..As a functional consequence, the lipogenic effect of SREBP2(N) in liver cells was suppressed by ATF6(N). Our results provide a novel mechanism by which ATF6 antagonizes SREBP2 to regulate the homeostasis of lipid and glucose... - AMP-activated protein kinase functionally phosphorylates endothelial nitric oxide synthase Ser633Zhen Chen
Division of Biomedical Sciences, University of California, Riverside, CA 92521, USA
Circ Res 104:496-505. 2009..Nano-LC/MS/MS confirmed that eNOS purified from AICAR-treated ECs was phosphorylated at both Ser633 and Ser1177. Our results indicate that AMPK phosphorylation of eNOS Ser633 is a functional signaling event for NO bioavailability in ECs... - The role of p53 deacetylation in p21Waf1 regulation by laminar flowLingfang Zeng
Division of Biomedical Sciences, University of California, Riverside 92521, USA
J Biol Chem 278:24594-9. 2003..The regulation of the p53-p21Waf1 pathway by laminar flow was further supported by observations that flow caused an increase of p21Waf1 level in the wild-type HCT116 (p53+/+) cells but not in the p53-null HCT116 cells... - Flow-Dependent Regulation of Kruppel-Like Factor 2 Is Mediated by MicroRNA-92aWei Wu
Division of Biomedical Sciences, University of California Riverside, 92521, USA
Circulation 124:633-41. 2011..MicroRNAs (miRNAs) are noncoding small RNAs that regulate gene expression at the posttranscriptional level. We examined the role of miRNAs, particularly miR-92a, in the atheroprotective flow-regulated KLF2... - Glucagon regulates ACC activity in adipocytes through the CAMKKβ/AMPK pathwayI Chen Peng
Division of Biomedical Sciences, University of California, Riverside, 92521, USA
Am J Physiol Endocrinol Metab 302:E1560-8. 2012..These results indicate that CaMKKβ/AMPK signaling is an important molecular component in regulating lipid metabolism in adipocytes responding to glucagon and could be a therapeutic target for the dysregulation of energy storage... - Role of microRNA-23b in flow-regulation of Rb phosphorylation and endothelial cell growthKuei Chun Wang
Department of Bioengineering and Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA
Proc Natl Acad Sci U S A 107:3234-9. 2010..Our findings indicate that PS suppresses EC proliferation through the regulation of miR-23b and provide insights into the role of miRs in mechanotransduction... - AMP-activated protein kinase is involved in endothelial NO synthase activation in response to shear stressYingjia Zhang
Division of Biomedical Sciences, University of California, Riverside, CA 92521 0121, USA
Arterioscler Thromb Vasc Biol 26:1281-7. 2006..In this study, we investigate the regulation of the AMPK-eNOS pathway in vascular endothelial cells (ECs) by shear stress and the activation of aortic AMPK in a mouse model with a high level of voluntary running (High-Runner)... - Intervertebral disc degeneration: the role of the mitochondrial pathway in annulus fibrosus cell apoptosis induced by overloadFrancois Rannou
Division of Biomedical Sciences, University of California, Riverside, California 92521 0121, USA
Am J Pathol 164:915-24. 2004..Our results from human samples, mouse models, and annulus fibrosus culture experiments demonstrate that the mechanical overload-induced IVD degeneration is mediated through the mitochondrial apoptotic pathway in IVD cells... - Sterol-responsive element-binding protein (SREBP) 2 down-regulates ATP-binding cassette transporter A1 in vascular endothelial cells: a novel role of SREBP in regulating cholesterol metabolismLingfang Zeng
Division of Biomedical Sciences, University of California, Riverside, Riverside, California 92521, USA
J Biol Chem 279:48801-7. 2004..Thus, this SREBP-regulated mechanism may control the efflux of cholesterol, which is a newly defined function of SREBP2 in ECs in addition to its role in cholesterol uptake and biosynthesis... 
microRNA: a master regulator of cellular processes for bioengineering systemsWei Sun
Division of Biomedical Sciences, University of California, Riverside, California 92521, USA
Annu Rev Biomed Eng 12:1-27. 2010....- Rho-ROCK-LIMK-cofilin pathway regulates shear stress activation of sterol regulatory element binding proteinsTong Lin
Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521-0121, USA
Circ Res 92:1296-304. 2003..By enhancing the SREBP-mediated cholesterol metabolism, this unique mechanism may contribute to endothelial cell functions under flow... - Vascular endothelial growth factor activation of sterol regulatory element binding protein: a potential role in angiogenesisRui-Hai Zhou
Division of Biomedical Sciences, University of California, Riverside Riverside 92521-0121, USA
Circ Res 95:471-8. 2004..SREBP inhibition also markedly suppressed VEGF-induced angiogenesis in chick embryos. In summary, this study identifies SREBPs as the key molecules in regulating angiogenesis in response to VEGF... - Stent implantation activates Akt in the vessel wall: role of mechanical stretch in vascular smooth muscle cellsRui-Hai Zhou
Division of Biomedical Sciences, University of California, Riverside, Riverside, Calif 92521-0121, USA
Arterioscler Thromb Vasc Biol 23:2015-20. 2003..CONCLUSIONS: Mechanical stretch of the vascular wall during stent deployment may contribute to ISR by activating the Akt pathway... - Interplay between integrins and FLK-1 in shear stress-induced signalingYingxiao Wang
Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, California 92093, USA
Am J Physiol Cell Physiol 283:C1540-7. 2002..Integrins are essential for the mechanical activation of Flk-1 by shear stress but not for the chemical activation of Flk-1 by VEGF... - Shear stress activation of SREBP1 in endothelial cells is mediated by integrinsYi Liu
Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, USA
Arterioscler Thromb Vasc Biol 22:76-81. 2002..These results demonstrate that integrin signaling plays a critical role in the modulation of SREBP in ECs in response to shear stress... - Fluorescence proteins, live-cell imaging, and mechanobiology: seeing is believingYingxiao Wang
Department of Bioengineering and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Urbana, IL 61801, USA
Annu Rev Biomed Eng 10:1-38. 2008..These live-cell imaging technologies, which represent a frontier area in biomedical engineering, can shed new light on the mechanisms regulating mechanobiology at cellular and molecular levels in normal and pathophysiological conditions... - Visualization of polarized membrane type 1 matrix metalloproteinase activity in live cells by fluorescence resonance energy transfer imagingMingxing Ouyang
Department of Bioengineering and the Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana Champaign, Illinois 61801, USA
J Biol Chem 283:17740-8. 2008..Hence, the MT1-MMP biosensor provides a powerful tool for characterizing the molecular processes underlying the spatiotemporal regulation of this critical class of enzymes... - Laminar shear stress regulates liver X receptor in vascular endothelial cellsMinjia Zhu
Department of Physiology and Pathophysiology, Peking University, Health Sciences Center, Beijing, China 100083
Arterioscler Thromb Vasc Biol 28:527-33. 2008..The liver X receptors (LXRs) regulate a set of genes involved in lipid metabolism and reverse cholesterol transport. We investigated the mechanism by which shear stress regulates LXR in vascular endothelial cells (ECs)... - Cholesterol loading increases the translocation of ATP synthase beta chain into membrane caveolae in vascular endothelial cellsTengke Wang
Institute of Vascular Medicine, Peking University Third Hospital, Beijing 100083, China
Biochim Biophys Acta 1761:1182-90. 2006..Thus, the ectopic localization of ATPS-beta may participate in the energy balance of cells in response to the change in intracellular cholesterol levels... - Oxidized LDL downregulates ATP-binding cassette transporter-1 in human vascular endothelial cells via inhibiting liver X receptor (LXR)Yi Zhu
Department of Physiology, Key Laboratory of Molecular Cardiovascular Sciences of Education Ministry, Health Science Center, Peking University, Beijing 100083, China
Cardiovasc Res 68:425-32. 2005..This report describes the effect of copper-catalyzed oxLDL on the regulation of ABCA1 in human endothelial cells (ECs)... - Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathwayYingxiao Wang
Department of Bioengineering, The Whitaker Institute of Biomedical Engineering, University of California San Diego, La Jolla 92093 0427, USA
Am J Physiol Heart Circ Physiol 286:H685-92. 2004..Therefore, our results indicate that the signaling events induced by shear stress and VEGF converge at the membrane receptor Flk-1 and that these stimuli share the Flk-1/Cbl/Akt pathway in activating IKK activation... - Role for sterol regulatory element-binding protein in activation of endothelial cells by phospholipid oxidation productsMichael Yeh
Department of Medicine, Geffen School of Medicine at University of California Los Angeles, USA
Circ Res 95:780-8. 2004..The current studies suggest a novel role for endothelial cholesterol depletion and subsequent SREBP activation in inflammatory processes in which phospholipid oxidation products accumulate...
Research Grants
- Shear Stress, AMPK, and Endothelial FunctionsJohn Shyy; Fiscal Year: 2009..The proposal will significantly increase our understanding of mechanisms by which blood flow patterns interplay with hyperlipidemia in atherogenesis, which are likely to contribute to novel therapies for its prevention and/or treatment. ..
- Shear stress, SIRT1, and Arterial StiffeningJohn Y J Shyy; Fiscal Year: 2010..Results from the proposed experiments are likely to contribute to novel therapies for its prevention and/or treatment. ..
- STRAIN INDUCTION OF VSMC PROLIFERATION AND APOPTOSISJohn Shyy; Fiscal Year: 2002....
- Flow Regulation of SREBP in AtherosclerosisJohn Shyy; Fiscal Year: 2007..By combining bioengineering, vascular biology, and transgenic approaches, this program will generate new insights into the molecular and mechanical bases of local flow patterns in atherogenesis. ..
- Shear Stress, AMPK, and Endothelial FunctionsJohn Y J Shyy; Fiscal Year: 2010....
- SHEAR STRESS ENHANCEMENT OF SMOOTH MUSCLE CELL MIGRATIONJohn Shyy; Fiscal Year: 2003....