Trevor J Shuttleworth

Summary

Affiliation: University of Rochester
Country: USA

Publications

  1. pmc STIM and Orai proteins and the non-capacitative ARC channels
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Front Biosci (Landmark Ed) 17:847-60. 2012
  2. ncbi request reprint Calcium entry and the control of calcium oscillations
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Biochem Soc Trans 31:916-9. 2003
  3. pmc Orai3--the 'exceptional' Orai?
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 590:241-57. 2012
  4. pmc The molecular architecture of the arachidonate-regulated Ca2+-selective ARC channel is a pentameric assembly of Orai1 and Orai3 subunits
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 587:4181-97. 2009
  5. pmc Arachidonic acid, ARC channels, and Orai proteins
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Cell Calcium 45:602-10. 2009
  6. ncbi request reprint ARC channels: a novel pathway for receptor-activated calcium entry
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Physiology (Bethesda) 19:355-61. 2004
  7. pmc STIM1 and the noncapacitative ARC channels
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Cell Calcium 42:183-91. 2007
  8. ncbi request reprint Receptor-activated calcium entry channels--who does what, and when?
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 711, Rochester, NY 14642, USA
    Sci STKE 2004:pe40. 2004
  9. pmc STIM1 regulates Ca2+ entry via arachidonate-regulated Ca2+-selective (ARC) channels without store depletion or translocation to the plasma membrane
    Olivier Mignen
    Department of Pharmacology and Physiology, Box 711, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 579:703-15. 2007
  10. pmc Both Orai1 and Orai3 are essential components of the arachidonate-regulated Ca2+-selective (ARC) channels
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 586:185-95. 2008

Research Grants

  1. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2004
  2. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2007
  3. Signaling Pathways in Salivary Gland Fluid Secretion
    Trevor Shuttleworth; Fiscal Year: 2009
  4. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1991
  5. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1990
  6. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1993
  7. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2000
  8. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor J Shuttleworth; Fiscal Year: 2010

Collaborators

Detail Information

Publications35

  1. pmc STIM and Orai proteins and the non-capacitative ARC channels
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Front Biosci (Landmark Ed) 17:847-60. 2012
    ..There is increasing evidence that the activity of these channels plays a critical role in a variety of different cellular activities...
  2. ncbi request reprint Calcium entry and the control of calcium oscillations
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Biochem Soc Trans 31:916-9. 2003
    ..At the same time the ARC channels are turned off, resulting in what we have described as a reciprocal regulation of these two distinct Ca(2+) entry pathways...
  3. pmc Orai3--the 'exceptional' Orai?
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 590:241-57. 2012
    ....
  4. pmc The molecular architecture of the arachidonate-regulated Ca2+-selective ARC channel is a pentameric assembly of Orai1 and Orai3 subunits
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 587:4181-97. 2009
    ....
  5. pmc Arachidonic acid, ARC channels, and Orai proteins
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Cell Calcium 45:602-10. 2009
    ....
  6. ncbi request reprint ARC channels: a novel pathway for receptor-activated calcium entry
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    Physiology (Bethesda) 19:355-61. 2004
    ..These signals activate calcineurin, which in turn inhibits the ARC channels, resulting in a "reciprocal regulation" of these two distinct Ca2+-entry pathways that may have important functional implications for the cell...
  7. pmc STIM1 and the noncapacitative ARC channels
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Cell Calcium 42:183-91. 2007
    ..Precisely how this same protein can act in such separate and specific ways on these different pathways of agonist-activated Ca(2+)entry remains an intriguing, yet currently unresolved, question...
  8. ncbi request reprint Receptor-activated calcium entry channels--who does what, and when?
    Trevor J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 711, Rochester, NY 14642, USA
    Sci STKE 2004:pe40. 2004
    ..These different channels are activated in distinct ways and operate under different conditions of stimulation...
  9. pmc STIM1 regulates Ca2+ entry via arachidonate-regulated Ca2+-selective (ARC) channels without store depletion or translocation to the plasma membrane
    Olivier Mignen
    Department of Pharmacology and Physiology, Box 711, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 579:703-15. 2007
    ..These data demonstrate that STIM1 is a more universal regulator of Ca2+ entry pathways than previously thought, and appears to have multiple modes of action...
  10. pmc Both Orai1 and Orai3 are essential components of the arachidonate-regulated Ca2+-selective (ARC) channels
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 586:185-95. 2008
    ....
  11. pmc Agonist activation of arachidonate-regulated Ca2+-selective (ARC) channels in murine parotid and pancreatic acinar cells
    Olivier Mignen
    Department of Pharmacology and Physiology, Box 711, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 564:791-801. 2005
    ..This is consistent with previous reports indicating that carbachol-induced [Ca(2+)](i) signals in these cells are much more dependent on Ca(2+) entry than are the cholecystokinin-induced responses...
  12. ncbi request reprint Modulation of [Ca2+]i signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells
    Jason I E Bruce
    Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Biol Chem 279:12909-17. 2004
    ..These effects may profoundly affect a variety of nuclear processes in parotid acinar cells while facilitating efficient fluid secretion...
  13. pmc Arachidonate-regulated Ca2+-selective (ARC) channel activity is modulated by phosphorylation and involves an A-kinase anchoring protein
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 567:787-98. 2005
    ....
  14. ncbi request reprint Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells
    Jason I E Bruce
    Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, New York 14642, USA
    J Biol Chem 277:48172-81. 2002
    ..Tight regulation of both Ca(2+) release and Ca(2+) efflux may represent a general feature of the mechanism whereby cAMP improves the fidelity and specificity of Ca(2+) signaling...
  15. pmc Orai1 subunit stoichiometry of the mammalian CRAC channel pore
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 586:419-25. 2008
    ..In this way we were able to demonstrate, for the first time, that the functional CRAC channel pore is formed by a tetrameric assembly of Orai1 subunits...
  16. pmc Cytosolic Ca(2+) and Ca(2+)-activated Cl(-) current dynamics: insights from two functionally distinct mouse exocrine cells
    David R Giovannucci
    Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Physiol 540:469-84. 2002
    ..These data reveal specializations of common modules of Ca(2+)-release machinery and subsequent effector activation that are specifically suited to the distinct functional roles of these two related cell types...
  17. doi request reprint The ARC channel--an endogenous store-independent Orai channel
    Jill L Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York, USA
    Curr Top Membr 71:125-48. 2013
    ....
  18. pmc A plasma membrane-targeted cytosolic domain of STIM1 selectively activates ARC channels, an arachidonate-regulated store-independent Orai channel
    Jill L Thompson
    Department of Pharmacology, University of Rochester Medical Center, Rochester, NY, USA
    Channels (Austin) 6:370-8. 2012
    ....
  19. pmc Molecular basis of activation of the arachidonate-regulated Ca2+ (ARC) channel, a store-independent Orai channel, by plasma membrane STIM1
    Jill L Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 591:3507-23. 2013
    ..Based on these findings, we propose that the likely role of arachidonic acid lies in inducing the actual gating of the channel...
  20. pmc Orai channel-dependent activation of phospholipase C-δ: a novel mechanism for the effects of calcium entry on calcium oscillations
    Jill L Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    J Physiol 589:5057-69. 2011
    ....
  21. ncbi request reprint Calcineurin directs the reciprocal regulation of calcium entry pathways in nonexcitable cells
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 278:40088-96. 2003
    ..Thus, calcineurin is the key mediator of the reciprocal regulation of these co-existing channels, allowing each to play a unique and non-overlapping role in Ca2+ signaling...
  22. ncbi request reprint Carboxyamidotriazole-induced inhibition of mitochondrial calcium import blocks capacitative calcium entry and cell proliferation in HEK-293 cells
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester, 601 Elmwood Avenue, Rochester, NY 14642, USA
    J Cell Sci 118:5615-23. 2005
    ....
  23. ncbi request reprint Ca2+ selectivity and fatty acid specificity of the noncapacitative, arachidonate-regulated Ca2+ (ARC) channels
    Olivier Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, New York 14642, USA
    J Biol Chem 278:10174-81. 2003
    ..Whether this involves a direct action of arachidonic acid on the channel protein itself or an action on some intermediary molecule is, at present, unclear...
  24. pmc The Orai1 severe combined immune deficiency mutation and calcium release-activated Ca2+ channel function in the heterozygous condition
    Jill L Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 284:6620-6. 2009
    ....
  25. pmc The N-terminal domain of Orai3 determines selectivity for activation of the store-independent ARC channel by arachidonic acid
    Jill Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, NY, USA
    Channels (Austin) 4:398-410. 2010
    ....
  26. ncbi request reprint Vertebrate salt glands: short- and long-term regulation of function
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, New York 14642, USA
    J Exp Zool 283:689-701. 1999
    ..This tuning of the signal involves often rather subtle changes in the overall signaling pathway that are part of the adaptive differentiation process...
  27. ncbi request reprint Muscarinic receptor activation of arachidonate-mediated Ca2+ entry in HEK293 cells is independent of phospholipase C
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 273:32636-43. 1998
    ....
  28. ncbi request reprint What drives calcium entry during [Ca2+]i oscillations?--challenging the capacitative model
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, New York, USA
    Cell Calcium 25:237-46. 1999
    ....
  29. ncbi request reprint Discriminating between capacitative and arachidonate-activated Ca(2+) entry pathways in HEK293 cells
    T J Shuttleworth
    Department of Pharmacology, University of Rochester School of Medicine, Rochester, New York 14642, USA
    J Biol Chem 274:31174-8. 1999
    ..These data demonstrate that the arachidonate-activated entry of Ca(2+) occurs via an entirely distinct influx pathway...
  30. ncbi request reprint Permeation of monovalent cations through the non-capacitative arachidonate-regulated Ca2+ channels in HEK293 cells. Comparison with endogenous store-operated channels
    O Mignen
    Department of Pharmacology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 276:21365-74. 2001
    ..However, the clear differences between the properties of these currents through ARC and SOC channels in the same cell confirm that these represent distinct conductances...
  31. ncbi request reprint Intracellular Ca2+ signalling in secretory cells
    T J Shuttleworth
    Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry, NY 14642, USA
    J Exp Biol 200:303-14. 1997
    ..In this way, the two components responsible for the elevation of [Ca2+]i are intimately related and their dual effects closely coordinated, resulting in the finely tuned control of agonist-induced changes in [Ca2+]i...
  32. ncbi request reprint Phosphorylation of inositol 1,4,5-trisphosphate receptors in parotid acinar cells. A mechanism for the synergistic effects of cAMP on Ca2+ signaling
    Jason I E Bruce
    Department of Pharmacology and Physiology, School of Medicine and Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 277:1340-8. 2002
    ..In addition, this report supports the emerging consensus that phosphorylation at the level of the Ca(2+) release machinery is a broadly important mechanism by which cells can regulate Ca(2+)-mediated processes...
  33. pmc How many Orai's does it take to make a CRAC channel?
    Jill L Thompson
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, USA
    Sci Rep 3:1961. 2013
    ....
  34. ncbi request reprint Reciprocal regulation of capacitative and arachidonate-regulated noncapacitative Ca2+ entry pathways
    O Mignen
    Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, New York 14642, USA
    J Biol Chem 276:35676-83. 2001
    ..Importantly, these data show that at physiologically relevant levels of stimulation, it is the noncapacitative ARC channels that provide the predominant route for the agonist-activated entry of Ca(2+)...
  35. pmc A mathematical model of fluid secretion from a parotid acinar cell
    Elan Gin
    Department of Mathematics, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    J Theor Biol 248:64-80. 2007
    ..We find that [Ca(2+)] oscillations lead to oscillations in fluid flow, and that the rate of fluid flow is regulated by the average calcium concentration and not the frequency of the oscillations...

Research Grants25

  1. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2004
    ....
  2. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2007
    ....
  3. Signaling Pathways in Salivary Gland Fluid Secretion
    Trevor Shuttleworth; Fiscal Year: 2009
    ..abstract_text> ..
  4. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1991
    ..Our aim is to reproduce this expression system in culture, thereby providing a unique system for studying the specific nature of the receptor-activated Ca2+ entry mechanism, its expression and control...
  5. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1990
    ....
  6. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 1993
    ..Our aim is to reproduce this expression system in culture, thereby providing a unique system for studying the specific nature of the receptor-activated Ca2+ entry mechanism, its expression and control...
  7. RECEPTOR REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor Shuttleworth; Fiscal Year: 2000
    ..It may also permit the development of pharmacological agents that can be used to manipulate or modify this funddamental and widely distributed process in clinically relevant ways. ..
  8. RECEPTOR-REGULATED CALCIUM ENTRY IN EXOCRINE SECRETION
    Trevor J Shuttleworth; Fiscal Year: 2010
    ..Defining the molecular bases of the relevant entry pathways and their regulation, will undoubtedly help elucidate the critical mechanisms involved in their activity, and identify potential targets for their clinical manipulation. ..