Affiliation: University of Pittsburgh
Ko S, Shin D. Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration. Methods Mol Biol. 2019;1905:83-90 pubmed publisher
..Here, we describe a chemical screening method using the zebrafish model for identifying small molecules that can modulate LPC-driven liver regeneration. ..
Ko S, Russell J, Tian J, Gao C, Kobayashi M, Feng R, et al
. Hdac1 Regulates Differentiation of Bipotent Liver Progenitor Cells During Regeneration via Sox9b and Cdk8. Gastroenterology. 2019;156:187-202.e14 pubmed publisher
..These pathways might be manipulated to induce LPC differentiation for treatment of patients with advanced liver diseases. ..
Russell J, Ko S, Saggi H, Singh S, Poddar M, Shin D, et al
. Bromodomain and Extraterminal (BET) Proteins Regulate Hepatocyte Proliferation in Hepatocyte-Driven Liver Regeneration. Am J Pathol. 2018;188:1389-1405 pubmed publisher
..In both models, Wnt signaling was prominently suppressed by JQ1. Our results show that BET proteins regulate hepatocyte proliferation-driven liver regeneration, and Wnt signaling is particularly sensitive to BET protein inhibition. ..
Choi T, Khaliq M, Ko S, So J, Shin D. Hepatocyte-specific ablation in zebrafish to study biliary-driven liver regeneration. J Vis Exp. 2015;:e52785 pubmed publisher
..Moreover, these methods can be applied to chemical screens to identify small molecules that augment or suppress liver regeneration. ..
Shin D, Weidinger G, Moon R, Stainier D. Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mech Dev. 2012;128:525-35 pubmed publisher
..Altogether, these studies reveal that there is more than one way to form a liver, and provide molecular insights into the phenomenon of tissue plasticity. ..
Choi T, Ninov N, Stainier D, Shin D. Extensive conversion of hepatic biliary epithelial cells to hepatocytes after near total loss of hepatocytes in zebrafish. Gastroenterology. 2014;146:776-88 pubmed publisher
..To investigate this issue, we established a zebrafish model of liver regeneration in which the extent of hepatocyte ablation can be controlled...
Khaliq M, Choi T, So J, Shin D. Id2a is required for hepatic outgrowth during liver development in zebrafish. Mech Dev. 2015;138 Pt 3:399-414 pubmed publisher
..Altogether, these data support the role of Id2a as an important regulator of hepatic outgrowth via modulation of hepatoblast proliferation and survival during liver development in zebrafish. ..
Ko S, Choi T, Russell J, So J, Monga S, Shin D. Bromodomain and extraterminal (BET) proteins regulate biliary-driven liver regeneration. J Hepatol. 2016;64:316-325 pubmed publisher
..BET proteins regulate BEC-driven liver regeneration at multiple steps: BEC dedifferentiation, HB-LC proliferation, the proliferation of newly generated hepatocytes, and hepatocyte maturation. ..