Ramin Shiekhattar

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. ncbi request reprint MicroRNA biogenesis: isolation and characterization of the microprocessor complex
    Richard I Gregory
    Gene Expression and Regulation Program, and Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA, USA
    Methods Mol Biol 342:33-47. 2006
  2. ncbi request reprint Dicer finds a new partner in transcriptional gene silencing
    Ramin Shiekhattar
    Center for Genomic Regulation, c Dr Aiguader, 88, 08003, Barcelona, Spain
    Mol Cell 27:519-20. 2007
  3. ncbi request reprint MicroRNA biogenesis and cancer
    Richard I Gregory
    Gene Expression and Regulation Program, and Molecular and Cellular Oncogenesis Program, Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 65:3509-12. 2005
  4. ncbi request reprint Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cell 128:877-87. 2007
  5. pmc Functional interplay between histone demethylase and deacetylase enzymes
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell Biol 26:6395-402. 2006
  6. ncbi request reprint Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination
    Min Gyu Lee
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Science 318:447-50. 2007
  7. pmc Histone H3 K4 demethylation during activation and attenuation of GAL1 transcription in Saccharomyces cerevisiae
    Kristin Ingvarsdottir
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 27:7856-64. 2007
  8. ncbi request reprint MicroRNA silencing through RISC recruitment of eIF6
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 447:823-8. 2007
  9. ncbi request reprint Isolation and characterization of histone H3 lysine 4 demethylase-containing complexes
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Methods 40:327-30. 2006
  10. ncbi request reprint Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II
    David Baillat
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Cell 123:265-76. 2005

Research Grants

  1. Functional elucidation of BRCA1-containing complexes
    Ramin Shiekhattar; Fiscal Year: 2007

Collaborators

Detail Information

Publications35

  1. ncbi request reprint MicroRNA biogenesis: isolation and characterization of the microprocessor complex
    Richard I Gregory
    Gene Expression and Regulation Program, and Molecular and Cellular Oncogenesis Program, The Wistar Institute, Philadelphia, PA, USA
    Methods Mol Biol 342:33-47. 2006
    ..We include a protocol for the in vitro analysis of pri-miRNA processing activity of the purified Microprocessor complex...
  2. ncbi request reprint Dicer finds a new partner in transcriptional gene silencing
    Ramin Shiekhattar
    Center for Genomic Regulation, c Dr Aiguader, 88, 08003, Barcelona, Spain
    Mol Cell 27:519-20. 2007
    ..2007) showed that fission yeast Dicer is physically and functionally associated with RNA-dependent RNA polymerase complex (RDRC), revealing a concerted mechanism in transcriptional gene silencing...
  3. ncbi request reprint MicroRNA biogenesis and cancer
    Richard I Gregory
    Gene Expression and Regulation Program, and Molecular and Cellular Oncogenesis Program, Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cancer Res 65:3509-12. 2005
    ..Microprocessor contains Drosha, an RNase III endonuclease, and DGCR8, a gene deleted in DiGeorge syndrome. We consider recent findings that link miRNA perturbation to cancer...
  4. ncbi request reprint Physical and functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR, a polycomb-like protein
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cell 128:877-87. 2007
    ....
  5. pmc Functional interplay between histone demethylase and deacetylase enzymes
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell Biol 26:6395-402. 2006
    ..Our studies not only reveal an intimate link between the histone demethylase and deacetylase enzymes but also identify histone demethylation as a secondary target of HDAC inhibitors...
  6. ncbi request reprint Demethylation of H3K27 regulates polycomb recruitment and H2A ubiquitination
    Min Gyu Lee
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Science 318:447-50. 2007
    ..Our results suggest a concerted mechanism for transcriptional activation in which cycles of H3K4 methylation by MLL2/3 are linked with the demethylation of H3K27 through UTX...
  7. pmc Histone H3 K4 demethylation during activation and attenuation of GAL1 transcription in Saccharomyces cerevisiae
    Kristin Ingvarsdottir
    Gene Expression and Regulation Program, The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Mol Cell Biol 27:7856-64. 2007
    ..Thus, analysis of lysine demethylation in yeast provides new insight into the physiological roles of jumonji demethylase enzymes...
  8. ncbi request reprint MicroRNA silencing through RISC recruitment of eIF6
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 447:823-8. 2007
    ..These results uncover an evolutionarily conserved function of the ribosome anti-association factor eIF6 in miRNA-mediated post-transcriptional silencing...
  9. ncbi request reprint Isolation and characterization of histone H3 lysine 4 demethylase-containing complexes
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Methods 40:327-30. 2006
    ..Here, we detail the methods used for the isolation and characterization of these histone demethylase complexes from a human stable cell line...
  10. ncbi request reprint Integrator, a multiprotein mediator of small nuclear RNA processing, associates with the C-terminal repeat of RNA polymerase II
    David Baillat
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Cell 123:265-76. 2005
    ..The Integrator complex is evolutionarily conserved in metazoans and directly interacts with the C-terminal domain of the RNA polymerase II largest subunit...
  11. pmc Functional dissection of the human TNRC6 (GW182-related) family of proteins
    David Baillat
    Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell Biol 29:4144-55. 2009
    ..In contrast, the C-terminal domain containing the RNA recognition motif plays a critical role in the silencing mediated by the TNRC6B protein...
  12. pmc Requirement for SNAPC1 in transcriptional responsiveness to diverse extracellular signals
    David Baillat
    The Wistar Institute, Philadelphia, Pennsylvania, USA
    Mol Cell Biol 32:4642-50. 2012
    ..These results highlight a role for SNAPC1 as a general transcriptional coactivator that functions through elongating RNAPII...
  13. pmc RNA editing of the microRNA-151 precursor blocks cleavage by the Dicer-TRBP complex
    Yukio Kawahara
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    EMBO Rep 8:763-9. 2007
    ....
  14. pmc Structural basis for ubiquitin recognition by the Otu1 ovarian tumor domain protein
    Troy Eugene Messick
    Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    J Biol Chem 283:11038-49. 2008
    ..Together, these studies provide new insights into ubiquitin binding and hydrolysis by yeast Otu1 and other OTU domain-containing proteins...
  15. ncbi request reprint Human RISC couples microRNA biogenesis and posttranscriptional gene silencing
    Richard I Gregory
    The Wistar Institute, Philadelphia, Pennsylvania 19104, USA
    Cell 123:631-40. 2005
    ..Importantly, ATP is not required for miRNA processing, RISC assembly, or multiple rounds of target-RNA cleavage. These results define the composition of RISC and demonstrate that miRNA processing and target-RNA cleavage are coupled...
  16. ncbi request reprint An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 437:432-5. 2005
    ..Together, these results highlight an essential role for CoREST in demethylation of H3K4 both in vitro and in vivo...
  17. pmc Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
    Guoping Da
    The Wistar Institute and Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA
    Proc Natl Acad Sci U S A 103:2057-62. 2006
    ..Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression...
  18. ncbi request reprint p53 is regulated by the lysine demethylase LSD1
    Jing Huang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 449:105-8. 2007
    ..Lysine methylation therefore provides similar regulatory complexity for non-histone proteins and for histones...
  19. ncbi request reprint Histone H3 lysine 4 demethylation is a target of nonselective antidepressive medications
    Min Gyu Lee
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Chem Biol 13:563-7. 2006
    ..These results attest to the effectiveness of tranylcypromine as a small molecular inhibitor of histone demethylation...
  20. pmc TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
    Thimmaiah P Chendrimada
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 436:740-4. 2005
    ..These results support a role of the Dicer-TRBP complex not only in miRNA processing but also as a platform for RISC assembly...
  21. ncbi request reprint Recruitment of MLL by HMG-domain protein iBRAF promotes neural differentiation
    Christopher Wynder
    The Wistar Institute, 3601 Spruce St, Philadelphia, PA 19104, USA
    Nat Cell Biol 7:1113-7. 2005
    ..Moreover, depletion of iBRAF abrogates recruitment of MLL and enhancement of histone H3K4 trimethylation. Together, these results indicate that the HMG-domain protein iBRAF has a key role in the initiation of neuronal differentiation...
  22. doi request reprint Activating RNAs associate with Mediator to enhance chromatin architecture and transcription
    Fan Lai
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 494:497-501. 2013
    ..Our results identify the human Mediator complex as the transducer of activating ncRNAs and highlight the importance of Mediator and activating ncRNA association in human disease...
  23. pmc Activation of BRCA1/BRCA2-associated helicase BACH1 is required for timely progression through S phase
    Easwari Kumaraswamy
    The Wistar Institute, Philadelphia, PA 19104, USA
    Mol Cell Biol 27:6733-41. 2007
    ..These results point to a critical role for BACH1 helicase activity not only in the timely progression through the S phase but also in maintaining genomic stability...
  24. pmc Human GTPases associate with RNA polymerase II to mediate its nuclear import
    Clément Carré
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Mol Cell Biol 31:3953-62. 2011
    ..Thus, GPN1/GPN3 define a new family of small GTPases that are specialized for the transport of RNA polymerase II into the nucleus...
  25. pmc Modulation of microRNA processing and expression through RNA editing by ADAR deaminases
    Weidong Yang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nat Struct Mol Biol 13:13-21. 2006
    ..Consequently, mature miRNA-142 expression levels increased substantially in ADAR1 null or ADAR2 null mice. Our results demonstrate a new function of RNA editing in the control of miRNA biogenesis...
  26. ncbi request reprint Linking transcriptional elongation and messenger RNA export to metastatic breast cancers
    Shanchun Guo
    Department of Medical Oncology, Fox Chase Cancer Center and Wistar Institute, Philadelphia, PA 19104, USA
    Cancer Res 65:3011-6. 2005
    ..These results not only identify hTREX84 as a prognosticator of breast cancer but also delineate human TREX complex as a target for therapeutic drugs against breast cancer...
  27. doi request reprint An operational definition of epigenetics
    Shelley L Berger
    Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Genes Dev 23:781-3. 2009
    ..It was evident that multiple mechanistic steps lead to the stable heritance of the epigenetic phenotype. Below we provide our view and interpretation of the proceedings at the meeting...
  28. ncbi request reprint The Microprocessor complex mediates the genesis of microRNAs
    Richard I Gregory
    The Wistar Institute, 3601 Spruce Street, Philadelphia, Pennsylvania 19104, USA
    Nature 432:235-40. 2004
    ..In vivo knock-down and in vitro reconstitution studies revealed that both components of this smaller complex, termed Microprocessor, are necessary and sufficient in mediating the genesis of miRNAs from the primary miRNA transcript...
  29. ncbi request reprint Functional analysis of Hes-1 in preadipocytes
    David A Ross
    Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Mol Endocrinol 20:698-705. 2006
    ..We conclude that Notch most likely blocks adipogenesis through the induction of Hes-1 homodimers, which repress transcription of key target genes...
  30. doi request reprint Noncoding RNAs and enhancers: complications of a long-distance relationship
    Ulf Andersson Orom
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Trends Genet 27:433-9. 2011
    ..In this review, we discuss recent findings on the role of long noncoding RNAs in transcriptional regulation, with an emphasis on new developments on the functional links between long noncoding RNAs and enhancers...
  31. ncbi request reprint Analysis of Nedd8-associated polypeptides: a model for deciphering the pathway for ubiquitin-like modifications
    Jessica A Norman
    Department of Cell and Molecular Biology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA
    Biochemistry 45:3014-9. 2006
    ..This purification scheme can be applied to other small ubiquitin-like proteins, especially those with limited protein targets such as the SUMOs (1, 2, and 3), Isg15, or FAT10...
  32. pmc Cell cycle regulation of chromatin at an origin of DNA replication
    Jing Zhou
    The Wistar Institute, Philadelphia, PA 19104, USA
    EMBO J 24:1406-17. 2005
    ..We conclude that an SNF2h-HDAC1/2 complex coordinates G1-specific chromatin remodeling and histone deacetylation with the DNA replication initiation process at OriP...
  33. doi request reprint Long noncoding RNAs with enhancer-like function in human cells
    Ulf Andersson Ørom
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Cell 143:46-58. 2010
    ..These results reveal an unanticipated role for a class of long ncRNAs in activation of critical regulators of development and differentiation...
  34. ncbi request reprint Chromatin modifiers and carcinogenesis
    Richard I Gregory
    Gene Expression and Regulation Program, and Molecular and Cellular Oncogenesis Program, Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Trends Cell Biol 14:695-702. 2004
    ....
  35. pmc Huntington's disease protein contributes to RNA-mediated gene silencing through association with Argonaute and P bodies
    Jeffrey N Savas
    Department of Microbiology and New York University Cancer Institute, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 105:10820-5. 2008
    ..These data suggest that the previously reported transcriptional deregulation in HD may be attributed in part to mutant Htt's role in post-transcriptional processes...

Research Grants3

  1. Functional elucidation of BRCA1-containing complexes
    Ramin Shiekhattar; Fiscal Year: 2007
    ..Aim 3 extends the work to analysis of the effect of BRCC and BACH1-BRCA1 complexes on regulation of late replicating DNA and heterochromatin structure. ..