Jay Shendure

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Exome-wide DNA capture and next generation sequencing in domestic and wild species
    Ted Cosart
    Department of Computer Science, University of Montana, Missoula, MT, USA
    BMC Genomics 12:347. 2011
  2. doi request reprint Next-generation DNA sequencing
    Jay Shendure
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195 5065, USA
    Nat Biotechnol 26:1135-45. 2008
  3. pmc Spectrum of MLL2 (ALR) mutations in 110 cases of Kabuki syndrome
    Mark C Hannibal
    Department of Pediatrics, University of Washington, Seattle, 98195, USA
    Am J Med Genet A 155:1511-6. 2011
  4. pmc A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, USA
    Methods 58:277-88. 2012
  5. pmc Estimating the human mutation rate using autozygosity in a founder population
    Catarina D Campbell
    Department of Genome Sciences, University of Washington, Seattle, USA
    Nat Genet 44:1277-81. 2012
  6. pmc Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome
    Sarah B Ng
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Genet 42:790-3. 2010
  7. doi request reprint Exome sequencing as a tool for Mendelian disease gene discovery
    Michael J Bamshad
    Department of Pediatrics, University of Washington, Health Sciences Building RR349, 1959 NE Pacific Street, Seattle, Washington 98195 6320, USA
    Nat Rev Genet 12:745-55. 2011
  8. ncbi request reprint Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions
    Joshua N Burton
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Biotechnol 31:1119-25. 2013
  9. pmc Noninvasive fetal genome sequencing: a primer
    Matthew W Snyder
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Prenat Diagn 33:547-54. 2013
  10. pmc The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, Washington 98115, USA
    Nature 500:207-11. 2013

Detail Information

Publications62

  1. pmc Exome-wide DNA capture and next generation sequencing in domestic and wild species
    Ted Cosart
    Department of Computer Science, University of Montana, Missoula, MT, USA
    BMC Genomics 12:347. 2011
    ..indicus, and Bison bison (wild bison). Our capture array has probes for 16,131 exons in 2,570 genes, including 203 candidate genes with known function and of interest for their association with disease and other fitness traits...
  2. doi request reprint Next-generation DNA sequencing
    Jay Shendure
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195 5065, USA
    Nat Biotechnol 26:1135-45. 2008
    ....
  3. pmc Spectrum of MLL2 (ALR) mutations in 110 cases of Kabuki syndrome
    Mark C Hannibal
    Department of Pediatrics, University of Washington, Seattle, 98195, USA
    Am J Med Genet A 155:1511-6. 2011
    ..These results are important for understanding the phenotypic consequences of MLL2 mutations for individuals and their families as well as for providing a basis for the identification of additional genes for Kabuki syndrome...
  4. pmc A genome-wide 3C-method for characterizing the three-dimensional architectures of genomes
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, USA
    Methods 58:277-88. 2012
    ..Hence, our method can be applied to decipher the 3D architectures of genomes. Here, we provide a detailed protocol for this method...
  5. pmc Estimating the human mutation rate using autozygosity in a founder population
    Catarina D Campbell
    Department of Genome Sciences, University of Washington, Seattle, USA
    Nat Genet 44:1277-81. 2012
    ..We observed a non-uniform distribution of heterozygous SNVs (both newly identified and known) in the autozygous segments (P = 0.001), which is suggestive of mutational hotspots or sites of long-range gene conversion...
  6. pmc Exome sequencing identifies MLL2 mutations as a cause of Kabuki syndrome
    Sarah B Ng
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Genet 42:790-3. 2010
    ..Our results strongly suggest that mutations in MLL2 are a major cause of Kabuki syndrome...
  7. doi request reprint Exome sequencing as a tool for Mendelian disease gene discovery
    Michael J Bamshad
    Department of Pediatrics, University of Washington, Health Sciences Building RR349, 1959 NE Pacific Street, Seattle, Washington 98195 6320, USA
    Nat Rev Genet 12:745-55. 2011
    ..These advances also set the stage for applying exome and whole-genome sequencing to facilitate clinical diagnosis and personalized disease-risk profiling...
  8. ncbi request reprint Chromosome-scale scaffolding of de novo genome assemblies based on chromatin interactions
    Joshua N Burton
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Biotechnol 31:1119-25. 2013
    ..Hi-C data can also be used to validate chromosomal translocations in cancer genomes...
  9. pmc Noninvasive fetal genome sequencing: a primer
    Matthew W Snyder
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Prenat Diagn 33:547-54. 2013
    ....
  10. pmc The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, Washington 98115, USA
    Nature 500:207-11. 2013
    ..These data provide an extensively phased, high-quality reference genome for past and future experiments relying on HeLa, and demonstrate the value of haplotype resolution for characterizing cancer genomes and epigenomes...
  11. pmc Sporadic autism exomes reveal a highly interconnected protein network of de novo mutations
    Brian J O'Roak
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Nature 485:246-50. 2012
    ..Combined with copy number variant (CNV) data, these results indicate extreme locus heterogeneity but also provide a target for future discovery, diagnostics and therapeutics...
  12. pmc Analysis of genetic inheritance in a family quartet by whole-genome sequencing
    Jared C Roach
    Institute for Systems Biology, Seattle, WA 98103, USA
    Science 328:636-9. 2010
    ..Family-based genome analysis enabled us to narrow the candidate genes for both of these Mendelian disorders to only four. Our results demonstrate the value of complete genome sequencing in families...
  13. pmc Haplotype-resolved genome sequencing of a Gujarati Indian individual
    Jacob O Kitzman
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Biotechnol 29:59-63. 2011
    ..This method also facilitates the analysis of structural variation, for example, to anchor novel insertions to specific locations and haplotypes...
  14. pmc Mutations in PIEZO2 cause Gordon syndrome, Marden-Walker syndrome, and distal arthrogryposis type 5
    Margaret J McMillin
    Department of Pediatrics, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 94:734-44. 2014
    ..0001). Collectively, although GS, DA5, and MWS have traditionally been considered separate disorders, our findings indicate that they are etiologically related and perhaps represent variable expressivity of the same condition. ..
  15. pmc GRIN2A mutations cause epilepsy-aphasia spectrum disorders
    Gemma L Carvill
    Department of Pediatrics, Division of Genetic Medicine, University of Washington, Seattle, Washington, USA
    Nat Genet 45:1073-6. 2013
    ..GRIN2A mutations are restricted to this group of cases, which has important ramifications for diagnostic testing and treatment and provides new insights into the pathogenesis of this debilitating group of conditions. ..
  16. pmc Capturing native long-range contiguity by in situ library construction and optical sequencing
    Jerrod J Schwartz
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 109:18749-54. 2012
    ....
  17. pmc Multiplex targeted sequencing identifies recurrently mutated genes in autism spectrum disorders
    Brian J O'Roak
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 338:1619-22. 2012
    ..Our data support associations between specific genes and reciprocal subphenotypes (CHD8-macrocephaly and DYRK1A-microcephaly) and replicate the importance of a β-catenin-chromatin-remodeling network to ASD etiology...
  18. pmc Single molecule molecular inversion probes for targeted, high-accuracy detection of low-frequency variation
    Joseph B Hiatt
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Genome Res 23:843-54. 2013
    ..3%-1.4%), and NRAS p.Q61R (colon, 4.7%). We anticipate that smMIP will be broadly adoptable as a practical and effective method for accurately detecting low-frequency mutations in both research and clinical settings...
  19. pmc Rapid and accurate large-scale genotyping of duplicated genes and discovery of interlocus gene conversions
    Xander Nuttle
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Methods 10:903-9. 2013
    ....
  20. pmc Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis
    Lea M Starita
    Howard Hughes Medical Institute, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 110:E1263-72. 2013
    ..Our results highlight the general utility of high-throughput mutagenesis in delineating the molecular basis of enzyme activity...
  21. pmc Informed consent for whole genome sequencing: a qualitative analysis of participant expectations and perceptions of risks, benefits, and harms
    Holly K Tabor
    Department of Pediatrics, University of Washington, Seattle, Washington, USA
    Am J Med Genet A 158:1310-9. 2012
    ..Web-based tools that facilitate participant management of their individual research results could accommodate such a framework...
  22. pmc Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Biol 11:R119. 2010
    ..We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing...
  23. pmc Exome sequencing in sporadic autism spectrum disorders identifies severe de novo mutations
    Brian J O'Roak
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Genet 43:585-9. 2011
    ..Our results show that trio-based exome sequencing is a powerful approach for identifying new candidate genes for ASDs and suggest that de novo mutations may contribute substantially to the genetic etiology of ASDs...
  24. pmc Diversity of human copy number variation and multicopy genes
    Peter H Sudmant
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 330:641-6. 2010
    ..Our approach makes ~1000 genes accessible to genetic studies of disease association...
  25. pmc Mutations in ECEL1 cause distal arthrogryposis type 5D
    Margaret J McMillin
    Department of Pediatrics, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 92:150-6. 2013
    ..Our results distinguish a second developmental pathway that causes congenital-contracture syndromes...
  26. pmc Analysis of 6,515 exomes reveals the recent origin of most human protein-coding variants
    Wenqing Fu
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Nature 493:216-20. 2013
    ....
  27. pmc Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing
    Andrew Adey
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Genome Res 22:1139-43. 2012
    ..We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-quality coverage of each strand...
  28. pmc Accurate gene synthesis with tag-directed retrieval of sequence-verified DNA molecules
    Jerrod J Schwartz
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Methods 9:913-5. 2012
    ..Dial-out PCR enables multiplex in vitro clone screening and is a compelling alternative to in vivo cloning and Sanger sequencing for accurate gene synthesis...
  29. pmc Massively parallel functional dissection of mammalian enhancers in vivo
    Rupali P Patwardhan
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Biotechnol 30:265-70. 2012
    ....
  30. pmc Noninvasive whole-genome sequencing of a human fetus
    Jacob O Kitzman
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Sci Transl Med 4:137ra76. 2012
    ....
  31. pmc Targeted enrichment of specific regions in the human genome by array hybridization
    Catherine Igartua
    University of Washington, Seattle, Washington, USA
    Curr Protoc Hum Genet . 2010
    ....
  32. pmc High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis
    Rupali P Patwardhan
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Biotechnol 27:1173-5. 2009
    ..The method may also serve as a rapid screening tool for regulatory element engineering in synthetic biology...
  33. pmc Extraordinary molecular evolution in the PRDM9 fertility gene
    James H Thomas
    Department of Genome Sciences, University of Washington, Seattle, Washington, United States of America
    PLoS ONE 4:e8505. 2009
    ..We suggest instead that PRDM9 is involved in some aspect of centromere segregation conflict and that rapidly evolving centromeric DNA drives changes in PRDM9 DNA-binding domains...
  34. pmc Targeted capture and massively parallel sequencing of 12 human exomes
    Sarah B Ng
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Nature 461:272-6. 2009
    ..This strategy may be extendable to diseases with more complex genetics through larger sample sizes and appropriate weighting of non-synonymous variants by predicted functional impact...
  35. doi request reprint Methods for genomic partitioning
    Emily H Turner
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195 5065, USA
    Annu Rev Genomics Hum Genet 10:263-84. 2009
    ..The successful development of genomic partitioning strategies will be key to taking full advantage of massively parallel sequencing, at least until resequencing of complete mammalian genomes becomes widely affordable...
  36. pmc IFRD1 is a candidate gene for SMNA on chromosome 7q22-q23
    Zoran Brkanac
    Department of Psychiatry and Behavioral Sciences, University of Washington School of Medicine, Seattle, WA 98104, USA
    Am J Hum Genet 84:692-7. 2009
    ..Mutation analysis of IFRD1 in additional patients with similar phenotypes is needed for demonstration of causality and further evaluation of its importance in neurological diseases...
  37. pmc A de novo convergence of autism genetics and molecular neuroscience
    Niklas Krumm
    Department of Genome Sciences, University of Washington, Seattle, WA, USA
    Trends Neurosci 37:95-105. 2014
    ..These pathways and genes significantly expand the neurobiological targets for study, and suggest a path for future genetic and functional studies...
  38. pmc A general framework for estimating the relative pathogenicity of human genetic variants
    Martin Kircher
    1 Department of Genome Sciences, University of Washington, Seattle, Washington, USA 2
    Nat Genet 46:310-5. 2014
    ..The ability of CADD to prioritize functional, deleterious and pathogenic variants across many functional categories, effect sizes and genetic architectures is unmatched by any current single-annotation method. ..
  39. pmc Germline missense variants in the BTNL2 gene are associated with prostate cancer susceptibility
    Liesel M FitzGerald
    Division of Public Health Sciences, University of Washington, Seattle, Washington, USA
    Cancer Epidemiol Biomarkers Prev 22:1520-8. 2013
    ..Rare, inherited mutations account for 5% to 10% of all prostate cancer cases. However, to date, few causative mutations have been identified...
  40. doi request reprint The expanding scope of DNA sequencing
    Jay Shendure
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Biotechnol 30:1084-94. 2012
    ..However, there remain many potentially high-impact applications of next-generation DNA sequencing that are not yet fully realized...
  41. pmc Trans genomic capture and sequencing of primate exomes reveals new targets of positive selection
    Renee D George
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Genome Res 21:1686-94. 2011
    ..These results demonstrate the power of second-generation sequencing in comparative genomics and greatly expand the repertoire of available primate coding sequences...
  42. pmc Gorilla genome structural variation reveals evolutionary parallelisms with chimpanzee
    Mario Ventura
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Genome Res 21:1640-9. 2011
    ..Our analysis suggests that the chimpanzee and gorilla genomes are structurally more derived than either orangutan or human genomes...
  43. pmc Exome sequencing identifies a spectrum of mutation frequencies in advanced and lethal prostate cancers
    Akash Kumar
    Department of Genome Sciences, University of Washington, Seattle, WA 98105, USA
    Proc Natl Acad Sci U S A 108:17087-92. 2011
    ..Our results also suggest that increasingly deep catalogs of human germline variation may challenge the necessity of sequencing matched tumor-normal pairs...
  44. ncbi request reprint Resolving genomic disorder-associated breakpoints within segmental DNA duplications using massively parallel sequencing
    Xander Nuttle
    Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington, USA
    Nat Protoc 9:1496-513. 2014
    ..Once established for a specific genomic disorder, it is possible to process thousands of DNA samples within as little as 3-4 weeks. ..
  45. pmc megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering
    Sandrine Boissel
    Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA, Center for Immunity and Immunotherapies, Seattle Children s Research Institute, Seattle, WA 98101, USA, Pregenen, Inc, Seattle, WA 98103, USA, Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA, CELLECTIS S A, Paris, 75013, France, Division of Basic Sciences, Fred Hutch Cancer Research Center, Seattle, WA 98109, USA, Department of Biochemistry, University of Washington, Seattle, WA 98195, USA, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA and Department of Immunology, University of Washington, Seattle, WA 98195, USA
    Nucleic Acids Res 42:2591-601. 2014
    ..This architecture allows the generation of extremely active and hyper-specific compact nucleases that are compatible with all current viral and nonviral cell delivery methods. ..
  46. pmc The million mutation project: a new approach to genetics in Caenorhabditis elegans
    Owen Thompson
    Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA
    Genome Res 23:1749-62. 2013
    ..All the strains are available through the Caenorhabditis Genetics Center and all the sequence changes have been deposited in WormBase and are available through an interactive website. ..
  47. doi request reprint Identification of novel HLA class II target epitopes for generation of donor-specific T regulatory cells
    Brad Stone
    Translational Research Program, Benaroya Research Institute, Seattle, Washington, USA
    Clin Immunol 145:153-60. 2012
    ..These data demonstrate the feasibility of identifying potentially therapeutic HLA class II minor antigens for generation of donor-specific T(R)...
  48. pmc Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections
    Stephen J Salipante
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
    PLoS ONE 8:e65226. 2013
    ..The described method is sufficiently rapid (theoretically compatible with same-day turnaround times) and inexpensive for routine clinical use...
  49. pmc Targeted resequencing in epileptic encephalopathies identifies de novo mutations in CHD2 and SYNGAP1
    Gemma L Carvill
    Department of Pediatrics, Division of Genetic Medicine, University of Washington, Seattle, WA, USA
    Nat Genet 45:825-30. 2013
    ..Implementation of this rapid and efficient method will change diagnosis and understanding of the molecular etiologies of these disorders. ..
  50. pmc Whole-genome analysis reveals that mutations in inositol polyphosphate phosphatase-like 1 cause opsismodysplasia
    Jennifer E Below
    Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA
    Am J Hum Genet 92:137-43. 2013
    ..Evaluation of 12 families with opsismodysplasia revealed that INPPL1 mutations explain ~60% of cases overall, including both of the families in our cohort with more than one affected child and 50% of the simplex cases...
  51. pmc Mammalian X upregulation is associated with enhanced transcription initiation, RNA half-life, and MOF-mediated H4K16 acetylation
    Xinxian Deng
    Department of Pathology, School of Medicine, University of Washington, Seattle, WA 98195, USA
    Dev Cell 25:55-68. 2013
    ....
  52. pmc Poxviruses deploy genomic accordions to adapt rapidly against host antiviral defenses
    Nels C Elde
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA
    Cell 150:831-41. 2012
    ....
  53. pmc De novo germline and postzygotic mutations in AKT3, PIK3R2 and PIK3CA cause a spectrum of related megalencephaly syndromes
    Jean Baptiste Rivière
    Center for Integrative Brain Research, Seattle Children s Hospital, Seattle, Washington, USA
    Nat Genet 44:934-40. 2012
    ....
  54. pmc De novo mutations in the actin genes ACTB and ACTG1 cause Baraitser-Winter syndrome
    Jean Baptiste Rivière
    Center for Integrative Brain Research, Seattle Children s Hospital, Seattle, Washington, USA
    Nat Genet 44:440-4, S1-2. 2012
    ....
  55. pmc Next generation sequence analysis for mitochondrial disorders
    Valeria Vasta
    Seattle Children s Research Institute, 1900 9th Ave, Seattle, WA 98101, USA
    Genome Med 1:100. 2009
    ..The large numbers of potential culprit genes, together with the little guidance offered by most clinical phenotypes as to which gene may be causative, are a great challenge for the molecular diagnosis of these disorders...
  56. pmc Massively parallel sequencing and rare disease
    Sarah B Ng
    Department of Genome Sciences, University of Washington School of Medicine, Seattle WA 98195, USA
    Hum Mol Genet 19:R119-24. 2010
    ..Here, we review the recent literature in the use of high-throughput sequence data and its analysis in the discovery of causal mutations for rare disorders...
  57. pmc Targeted enrichment and high-resolution digital profiling of mitochondrial DNA deletions in human brain
    Sean D Taylor
    Translational Research Program, Public Health Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave, Seattle, WA, 98109, USA
    Aging Cell 13:29-38. 2014
    ..Our data provide support for the hypothesis that expansion of pre-existing mutations is the primary factor contributing to age-related accumulation of mtDNA deletions. ..
  58. pmc Genome interrupted: sequencing of prostate cancer reveals the importance of chromosomal rearrangements
    Akash Kumar
    Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue N, Seattle, WA 98109, USA
    Genome Med 3:23. 2011
    ..Novel candidate prostate cancer genes were also identified, highlighting the importance of genome sequencing to identify oncogenic changes that are otherwise invisible to detection...
  59. pmc A three-dimensional model of the yeast genome
    Zhijun Duan
    Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, Washington 98195 8056, USA
    Nature 465:363-7. 2010
    ..Finally, we constructed a three-dimensional model of the yeast genome. Our findings provide a glimpse of the interface between the form and function of a eukaryotic genome...
  60. pmc Exome sequencing identifies the cause of a mendelian disorder
    Sarah B Ng
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Genet 42:30-5. 2010
    ..Exome sequencing of a small number of unrelated affected individuals is a powerful, efficient strategy for identifying the genes underlying rare mendelian disorders and will likely transform the genetic analysis of monogenic traits...
  61. pmc Parallel, tag-directed assembly of locally derived short sequence reads
    Joseph B Hiatt
    Department of Genome Sciences, University of Washington, Seattle, Washington, USA
    Nat Methods 7:119-22. 2010
    ..Subassembly may facilitate accurate de novo genome assembly and metagenome sequencing...
  62. pmc Global survey of escape from X inactivation by RNA-sequencing in mouse
    Fan Yang
    Department of Pathology, University of Washington, Seattle, Washington 98195, USA
    Genome Res 20:614-22. 2010
    ..Furthermore, this epigenetic mark is developmentally regulated for some mouse genes...