Gerry Shaw

Summary

Affiliation: University of Florida
Country: USA

Publications

  1. ncbi request reprint Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury
    Gerry Shaw
    Department of Neuroscience, University of Florida College of Medicine, Gainesville, USA
    Biochem Biophys Res Commun 336:1268-77. 2005
  2. ncbi request reprint Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells
    Gerry Shaw
    University of Florida College of Medicine, Department of Neuroscience, McKnight Brain Institute, Gainesville, Florida, USA
    FASEB J 16:869-71. 2002
  3. ncbi request reprint Effects of acute ethanol exposure on regulatory mechanisms of Bcl-2-associated apoptosis promoter, bad, in neonatal rat cerebellum: differential effects during vulnerable and resistant developmental periods
    Kendra I Siler-Marsiglio
    Department of Neuroscience, Center for Alcohol Research, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA
    Alcohol Clin Exp Res 30:1031-8. 2006
  4. doi request reprint Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage
    Stephen B Lewis
    Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA
    J Neurosci Res 88:1475-84. 2010
  5. pmc Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomas
    Katharine M Laughlin
    Department of Pharmacology and Therapeutics, University of Florida College of Medicine, P O Box 100267, 1600 SW Archer Rd, Gainesville, FL 32610 0267, USA
    Pathol Oncol Res 15:437-44. 2009
  6. ncbi request reprint Effect of bax deletion on ethanol sensitivity in the neonatal rat cerebellum
    Marieta Barrow Heaton
    Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, 32610 0244, USA
    J Neurobiol 66:95-101. 2006
  7. ncbi request reprint Functional mechanisms of apoptosis-related proteins in neonatal rat cerebellum are differentially influenced by ethanol at postnatal days 4 and 7
    Kendra I Siler-Marsiglio
    McKnight Brain Institute, Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA
    J Neurosci Res 81:632-43. 2005
  8. ncbi request reprint Pycnogenol and vitamin E inhibit ethanol-induced apoptosis in rat cerebellar granule cells
    Kendra I Siler-Marsiglio
    University of Florida, McKnight Brain Institute, Department of Neuroscience, Gainesville, Florida 32611, USA
    J Neurobiol 59:261-71. 2004
  9. ncbi request reprint Ethanol effects on neonatal rat cortex: comparative analyses of neurotrophic factors, apoptosis-related proteins, and oxidative processes during vulnerable and resistant periods
    Marieta Barrow Heaton
    Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Box 100244, Gainesville, FL 32610 0244, USA
    Brain Res Dev Brain Res 145:249-62. 2003
  10. pmc Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells
    Ved P Sharma
    Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA
    J Leukoc Biol 84:440-7. 2008

Collaborators

Detail Information

Publications26

  1. ncbi request reprint Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury
    Gerry Shaw
    Department of Neuroscience, University of Florida College of Medicine, Gainesville, USA
    Biochem Biophys Res Commun 336:1268-77. 2005
    ..These findings suggest that serum levels of pNF-H immunoreactivity may be used to conveniently monitor neuronal damage and degeneration in experimental and presumably clinical situations...
  2. ncbi request reprint Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells
    Gerry Shaw
    University of Florida College of Medicine, Department of Neuroscience, McKnight Brain Institute, Gainesville, Florida, USA
    FASEB J 16:869-71. 2002
    ....
  3. ncbi request reprint Effects of acute ethanol exposure on regulatory mechanisms of Bcl-2-associated apoptosis promoter, bad, in neonatal rat cerebellum: differential effects during vulnerable and resistant developmental periods
    Kendra I Siler-Marsiglio
    Department of Neuroscience, Center for Alcohol Research, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA
    Alcohol Clin Exp Res 30:1031-8. 2006
    ..This dimerization prevents the neutralizing association of Bcl-xL with Bax, freeing Bax to perform in a prodeath manner. Caspase-dependent cleavage of Bad to a 15-kDa fragment increases its proapoptogenic capacity...
  4. doi request reprint Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage
    Stephen B Lewis
    Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA
    J Neurosci Res 88:1475-84. 2010
    ..We conclude that measurement of the CSF levels of these proteins reveals details of ASAH progression and recovery and predicts patient outcome...
  5. pmc Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomas
    Katharine M Laughlin
    Department of Pharmacology and Therapeutics, University of Florida College of Medicine, P O Box 100267, 1600 SW Archer Rd, Gainesville, FL 32610 0267, USA
    Pathol Oncol Res 15:437-44. 2009
    ..However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors...
  6. ncbi request reprint Effect of bax deletion on ethanol sensitivity in the neonatal rat cerebellum
    Marieta Barrow Heaton
    Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, 32610 0244, USA
    J Neurobiol 66:95-101. 2006
    ..Amelioration of ethanol-mediated increases in ROS production in the knock-outs may contribute to the observed effects...
  7. ncbi request reprint Functional mechanisms of apoptosis-related proteins in neonatal rat cerebellum are differentially influenced by ethanol at postnatal days 4 and 7
    Kendra I Siler-Marsiglio
    McKnight Brain Institute, Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA
    J Neurosci Res 81:632-43. 2005
    ..Such differential responsiveness likely plays a major role in the disparate ethanol vulnerability at these two postnatal ages...
  8. ncbi request reprint Pycnogenol and vitamin E inhibit ethanol-induced apoptosis in rat cerebellar granule cells
    Kendra I Siler-Marsiglio
    University of Florida, McKnight Brain Institute, Department of Neuroscience, Gainesville, Florida 32611, USA
    J Neurobiol 59:261-71. 2004
    ..These results suggest that both PYC and VE have the potential to act as therapeutic agents, antagonizing the induction of neuronal cell death by ethanol exposure...
  9. ncbi request reprint Ethanol effects on neonatal rat cortex: comparative analyses of neurotrophic factors, apoptosis-related proteins, and oxidative processes during vulnerable and resistant periods
    Marieta Barrow Heaton
    Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Box 100244, Gainesville, FL 32610 0244, USA
    Brain Res Dev Brain Res 145:249-62. 2003
    ....
  10. pmc Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cells
    Ved P Sharma
    Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA
    J Leukoc Biol 84:440-7. 2008
    ..These results imply that complementary regulation of PI3K and phosphate and tensin homolog (PTEN) is not the sole or dominant mechanism of PI(3,4,5)P3 polarization in HL-60 cells...
  11. pmc The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury
    Kevin J Anderson
    Department of Physiological Sciences, McKnight Brain Institute, University of Florida, Gainesville, Florida 32610, USA
    J Neurotrauma 25:1079-85. 2008
    ..These findings suggest that the measurement of blood levels of pNF-H is a convenient method for assessing neuropathology following TBI...
  12. ncbi request reprint Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistry
    Zeshan Ahmed
    Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida, USA
    J Histochem Cytochem 55:687-700. 2007
    ..Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia...
  13. pmc Hematopoietic- and neurologic-expressed sequence 1 (Hn1) depletion in B16.F10 melanoma cells promotes a differentiated phenotype that includes increased melanogenesis and cell cycle arrest
    Katharine M Laughlin
    Departments of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, FL 32610 0267, USA
    Differentiation 78:35-44. 2009
    ..These data, coupled with reports on Hn1 expression in regeneration and development, suggest that Hn1 functions as a suppressor of differentiation in cells undergoing repair or proliferation...
  14. pmc Noninvasive assessments of optic nerve neurodegeneration in transgenic mice with isolated optic neuritis
    Venu Talla
    Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA
    Invest Ophthalmol Vis Sci 54:4440-50. 2013
    ..To determine if phosphorylated neurofilament heavy chain (pNF-H) released into the bloodstream and the pattern ERG are noninvasive indicators of neurodegeneration in experimental optic neuritis...
  15. ncbi request reprint Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistry
    Hirotake Uchikado
    Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA
    Neurology 64:1658-9. 2005
  16. ncbi request reprint Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites
    Gerry Shaw
    Department of Neuroscience, University of Florida College of Medicine, McKnight Brain Institute, Gainesville, FL 32610, USA
    Biochem Biophys Res Commun 325:619-25. 2004
    ..Finally, we show biochemically that the more abundant and stable of these calpain fragments can also be detected in vivo...
  17. doi request reprint Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients
    Stephen B Lewis
    Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, Florida, USA
    J Cereb Blood Flow Metab 28:1261-71. 2008
    ..We conclude that the analysis of pNF-H levels in blood and CSF differentiates between patients with poor and favorable outcomes and also reveals several novel features of ASAH progression and recovery...
  18. ncbi request reprint Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem
    Jenafer Evans
    Department of Physiology, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA
    J Neurophysiol 87:1076-85. 2002
    ..These cells therefore have the immunological and electrophysiological properties of immature mitotic neurons and should be useful in a variety of future studies of neuronal differentiation and function...
  19. ncbi request reprint MARCKS is a natively unfolded protein with an inaccessible actin-binding site: evidence for long-range intramolecular interactions
    Hazel Tapp
    Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA
    J Biol Chem 280:9946-56. 2005
    ....
  20. ncbi request reprint The role of neurotrophic factors, apoptosis-related proteins, and endogenous antioxidants in the differential temporal vulnerability of neonatal cerebellum to ethanol
    Marieta Barrow Heaton
    Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, USA
    Alcohol Clin Exp Res 27:657-69. 2003
    ..We assessed basal levels of several substances which may be involved in differential temporal ethanol vulnerability in neonatal cerebellum, and analyzed alterations in these substances after early ethanol exposure...
  21. ncbi request reprint Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons
    Tanya S McGraw
    Department of Neuroscience, University of Florida College of Medicine and McKnight Brain Institute, Gainesville, Florida 32611, USA
    J Neurosci 22:4955-63. 2002
    ..We conclude that alpha-internexin upregulation in injured motoneurons suggests a role for this IF protein in neuronal regeneration...
  22. ncbi request reprint ANG II stimulation of neuritogenesis involves protein kinase B in brain neurons
    Hong Yang
    Department of Physiology and Functional Genomics, College of Medicine, University of Florida McKnight Brain Institute, Gainesville, FL 32610, USA
    Am J Physiol Regul Integr Comp Physiol 283:R107-14. 2002
    ..These observations demonstrate that PKB is directly involved in ANG II-mediated effects and may recruit both nuclear and cytoplasmic signaling systems for this action...
  23. ncbi request reprint Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kit
    Silas M J Morse
    University of Florida, Gainesville, FL 32610, USA
    Biotechniques 40:54, 56, 58. 2006
  24. ncbi request reprint Comparison of two ELISA methods for measuring levels of the phosphorylated neurofilament heavy chain
    Axel Petzold
    Department of Neuroimmunology, Institute of Neurology, University College London, Queen Square, London WC1N 3BG, United Kingdom
    J Immunol Methods 319:34-40. 2007
    ..The present study aims to cross-validate two different independently developed ELISA techniques for the quantification of the phosphorylated axonal forms of the neurofilament heavy chain (pNfH)...
  25. ncbi request reprint Extending the clinicopathological spectrum of neurofilament inclusion disease
    Keith A Josephs
    Department of Neurology, Mayo Clinic Rochester, Rochester, Minnesota, USA
    Acta Neuropathol 109:427-32. 2005
    ..This case extends the clinical and pathological spectrum of NFID to include cases with predominant parkinsonian and pyramidal features...
  26. pmc A neurotoxic peripherin splice variant in a mouse model of ALS
    Janice Robertson
    Centre for Research in Neurosciences, Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, H3G 1A4, Canada
    J Cell Biol 160:939-49. 2003
    ..This evidence suggests that expression of neurotoxic splice variants of peripherin may contribute to the neurodegenerative mechanism in ALS...