Genomes and Genes
Affiliation: University of Florida
- Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injuryGerry Shaw
Department of Neuroscience, University of Florida College of Medicine, Gainesville, USA
Biochem Biophys Res Commun 336:1268-77. 2005..These findings suggest that serum levels of pNF-H immunoreactivity may be used to conveniently monitor neuronal damage and degeneration in experimental and presumably clinical situations...
- Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cellsGerry Shaw
University of Florida College of Medicine, Department of Neuroscience, McKnight Brain Institute, Gainesville, Florida, USA
FASEB J 16:869-71. 2002....
- Effects of acute ethanol exposure on regulatory mechanisms of Bcl-2-associated apoptosis promoter, bad, in neonatal rat cerebellum: differential effects during vulnerable and resistant developmental periodsKendra I Siler-Marsiglio
Department of Neuroscience, Center for Alcohol Research, McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA
Alcohol Clin Exp Res 30:1031-8. 2006..This dimerization prevents the neutralizing association of Bcl-xL with Bax, freeing Bax to perform in a prodeath manner. Caspase-dependent cleavage of Bad to a 15-kDa fragment increases its proapoptogenic capacity...
- Hematopoietic- and neurologic-expressed sequence 1 expression in the murine GL261 and high-grade human gliomasKatharine M Laughlin
Department of Pharmacology and Therapeutics, University of Florida College of Medicine, P O Box 100267, 1600 SW Archer Rd, Gainesville, FL 32610 0267, USA
Pathol Oncol Res 15:437-44. 2009..However, tumors established from Hn1-depleted GL261 cells formed significantly smaller volumes than those established from control-treated cells. These data suggest a role for Hn1 in the biology of malignant brain tumors...
- Identification and preliminary characterization of ubiquitin C terminal hydrolase 1 (UCHL1) as a biomarker of neuronal loss in aneurysmal subarachnoid hemorrhageStephen B Lewis
Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA
J Neurosci Res 88:1475-84. 2010..We conclude that measurement of the CSF levels of these proteins reveals details of ASAH progression and recovery and predicts patient outcome...
- Effect of bax deletion on ethanol sensitivity in the neonatal rat cerebellumMarieta Barrow Heaton
Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, 32610 0244, USA
J Neurobiol 66:95-101. 2006..Amelioration of ethanol-mediated increases in ROS production in the knock-outs may contribute to the observed effects...
- Functional mechanisms of apoptosis-related proteins in neonatal rat cerebellum are differentially influenced by ethanol at postnatal days 4 and 7Kendra I Siler-Marsiglio
McKnight Brain Institute, Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA
J Neurosci Res 81:632-43. 2005..Such differential responsiveness likely plays a major role in the disparate ethanol vulnerability at these two postnatal ages...
- Pycnogenol and vitamin E inhibit ethanol-induced apoptosis in rat cerebellar granule cellsKendra I Siler-Marsiglio
University of Florida, McKnight Brain Institute, Department of Neuroscience, Gainesville, Florida 32611, USA
J Neurobiol 59:261-71. 2004..These results suggest that both PYC and VE have the potential to act as therapeutic agents, antagonizing the induction of neuronal cell death by ethanol exposure...
- Ethanol effects on neonatal rat cortex: comparative analyses of neurotrophic factors, apoptosis-related proteins, and oxidative processes during vulnerable and resistant periodsMarieta Barrow Heaton
Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Box 100244, Gainesville, FL 32610 0244, USA
Brain Res Dev Brain Res 145:249-62. 2003....
- Immunostaining evidence for PI(4,5)P2 localization at the leading edge of chemoattractant-stimulated HL-60 cellsVed P Sharma
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA
J Leukoc Biol 84:440-7. 2008..These results imply that complementary regulation of PI3K and phosphate and tensin homolog (PTEN) is not the sole or dominant mechanism of PI(3,4,5)P3 polarization in HL-60 cells...
- The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injuryKevin J Anderson
Department of Physiological Sciences, McKnight Brain Institute, University of Florida, Gainesville, Florida 32610, USA
J Neurotrauma 25:1079-85. 2008..These findings suggest that the measurement of blood levels of pNF-H is a convenient method for assessing neuropathology following TBI...
- Actin-binding proteins coronin-1a and IBA-1 are effective microglial markers for immunohistochemistryZeshan Ahmed
Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida, USA
J Histochem Cytochem 55:687-700. 2007..Because both coronin-1a and IBA-1 are actin-binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins are critical to dynamic properties of microglia...
- Hematopoietic- and neurologic-expressed sequence 1 (Hn1) depletion in B16.F10 melanoma cells promotes a differentiated phenotype that includes increased melanogenesis and cell cycle arrestKatharine M Laughlin
Departments of Pharmacology and Therapeutics, University of Florida, College of Medicine, Gainesville, FL 32610 0267, USA
Differentiation 78:35-44. 2009..These data, coupled with reports on Hn1 expression in regeneration and development, suggest that Hn1 functions as a suppressor of differentiation in cells undergoing repair or proliferation...
- Unbiased screen reveals ubiquilin-1 and -2 highly associated with huntingtin inclusionsNicola J Rutherford
Department of Neuroscience and Center for Translational Research in Neurodegenerative Disease, University of Florida, Gainesville, FL 32610, USA
Brain Res 1524:62-73. 2013..Thus, the co-localization of ubiquilin-2 with the huntingtin aggregates does not appear to facilitate aggregate removal. ..
- Noninvasive assessments of optic nerve neurodegeneration in transgenic mice with isolated optic neuritisVenu Talla
Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, Florida, USA
Invest Ophthalmol Vis Sci 54:4440-50. 2013..To determine if phosphorylated neurofilament heavy chain (pNF-H) released into the bloodstream and the pattern ERG are noninvasive indicators of neurodegeneration in experimental optic neuritis...
- Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patientsStephen B Lewis
Department of Neurological Surgery, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, Florida, USA
J Cereb Blood Flow Metab 28:1261-71. 2008..We conclude that the analysis of pNF-H levels in blood and CSF differentiates between patients with poor and favorable outcomes and also reveals several novel features of ASAH progression and recovery...
- Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sitesGerry Shaw
Department of Neuroscience, University of Florida College of Medicine, McKnight Brain Institute, Gainesville, FL 32610, USA
Biochem Biophys Res Commun 325:619-25. 2004..Finally, we show biochemically that the more abundant and stable of these calpain fragments can also be detected in vivo...
- Characterization of mitotic neurons derived from adult rat hypothalamus and brain stemJenafer Evans
Department of Physiology, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, FL 32610, USA
J Neurophysiol 87:1076-85. 2002..These cells therefore have the immunological and electrophysiological properties of immature mitotic neurons and should be useful in a variety of future studies of neuronal differentiation and function...
- Screening for neurofilament inclusion disease using alpha-internexin immunohistochemistryHirotake Uchikado
Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA
Neurology 64:1658-9. 2005
- ANG II stimulation of neuritogenesis involves protein kinase B in brain neuronsHong Yang
Department of Physiology and Functional Genomics, College of Medicine, University of Florida McKnight Brain Institute, Gainesville, FL 32610, USA
Am J Physiol Regul Integr Comp Physiol 283:R107-14. 2002..These observations demonstrate that PKB is directly involved in ANG II-mediated effects and may recruit both nuclear and cytoplasmic signaling systems for this action...
- Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneuronsTanya S McGraw
Department of Neuroscience, University of Florida College of Medicine and McKnight Brain Institute, Gainesville, Florida 32611, USA
J Neurosci 22:4955-63. 2002..We conclude that alpha-internexin upregulation in injured motoneurons suggests a role for this IF protein in neuronal regeneration...
- MARCKS is a natively unfolded protein with an inaccessible actin-binding site: evidence for long-range intramolecular interactionsHazel Tapp
Department of Medicine, University of Florida College of Medicine, Gainesville, Florida 32610, USA
J Biol Chem 280:9946-56. 2005....
- The role of neurotrophic factors, apoptosis-related proteins, and endogenous antioxidants in the differential temporal vulnerability of neonatal cerebellum to ethanolMarieta Barrow Heaton
Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine, Gainesville, USA
Alcohol Clin Exp Res 27:657-69. 2003..We assessed basal levels of several substances which may be involved in differential temporal ethanol vulnerability in neonatal cerebellum, and analyzed alterations in these substances after early ethanol exposure...
- Physiologically relevant factors influence tau phosphorylation by leucine-rich repeat kinase 2Matthew Hamm
Center for Translational Research in Neurodegenerative Disease, College of Medicine, University of Florida, Gainesville, Florida
J Neurosci Res 93:1567-80. 2015..These results suggest that cofactors such as MTs and cations in the cellular milieu have an important impact on LRRK2-tau interactions and resultant tau phosphorylation...
- Concurrent mRNA and protein extraction from the same experimental sample using a commercially available column-based RNA preparation kitSilas M J Morse
University of Florida, Gainesville, FL 32610, USA
Biotechniques 40:54, 56, 58. 2006
- A neurotoxic peripherin splice variant in a mouse model of ALSJanice Robertson
Centre for Research in Neurosciences, Research Institute of the McGill University Health Centre, McGill University, Montreal, Quebec, H3G 1A4, Canada
J Cell Biol 160:939-49. 2003..This evidence suggests that expression of neurotoxic splice variants of peripherin may contribute to the neurodegenerative mechanism in ALS...
- Extending the clinicopathological spectrum of neurofilament inclusion diseaseKeith A Josephs
Department of Neurology, Mayo Clinic Rochester, Rochester, Minnesota, USA
Acta Neuropathol 109:427-32. 2005..This case extends the clinical and pathological spectrum of NFID to include cases with predominant parkinsonian and pyramidal features...
- Comparison of two ELISA methods for measuring levels of the phosphorylated neurofilament heavy chainAxel Petzold
Department of Neuroimmunology, Institute of Neurology, University College London, Queen Square, London WC1N 3BG, United Kingdom
J Immunol Methods 319:34-40. 2007..The present study aims to cross-validate two different independently developed ELISA techniques for the quantification of the phosphorylated axonal forms of the neurofilament heavy chain (pNfH)...