- Factors affecting PCR-mediated recombinationSasha Shafikhani
Genencor International, 925 Page Mill Road, Palo Alto, CA 94304, USA
Environ Microbiol 4:482-6. 2002..subtilis as single strands. Combined, these results suggest that using Pfu DNA polymerase for amplification and B. subtilis as the host for transformation may significantly reduce chimera formation...