Research Topics
Species | Barbara ShacklettSummaryAffiliation: University of California Country: USA Publications
Research Grants
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Detail Information
Publications
Amplification of low-frequency antiviral CD8 T cell responses using autologous dendritic cellsMarie Larsson
Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY, USA
AIDS 16:171-80. 2002....- Detection of macaque perforin expression and release by flow cytometry, immunohistochemistry, ELISA, and ELISpotBartek Zuber
Mabtech, Box 1233, SE 131 28 Nacka, Sweden
J Immunol Methods 312:45-53. 2006..These macaque PFN-reactive mAbs and immunoassays will be valuable new tools for investigation of cytotoxic T lymphocyte (CTL) responses in non-human primate models of infectious diseases as well as for vaccine development... 
Can the new humanized mouse model give HIV research a boostBarbara L Shacklett
Department of Medical Microbiology and Immunology, Division of Infectious Diseases, School of Medicine, University of California, Davis, Davis, California, United States of America
PLoS Med 5:e13. 2008- Understanding the "lucky few": the conundrum of HIV-exposed, seronegative individualsBarbara L Shacklett
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
Curr HIV/AIDS Rep 3:26-31. 2006..This review describes the major findings on this important topic, with a focus on immunologic studies... - Immune responses to HIV in the female reproductive tract, immunologic parallels with the gastrointestinal tract, and research implicationsBarbara L Shacklett
Department of Medical Microbiology and Immunology, and Division of Infectious Diseases, Department of Internal Medicine, School of Medicine, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA
Am J Reprod Immunol 65:230-41. 2011..The susceptibility of the female reproductive tract to HIV infection and immunopathogenesis suggests important new avenues for further research... - Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infectionBarbara L Shacklett
Gladstone Institute of Virology and Immunology GIVI, Department of Medicine, University of California San Francisco, San Francisco, CA 94141, USA
J Immunol 173:641-8. 2004..Furthermore, the relative absence of armed effector cells may serve to protect the integrity of rectal mucosa under normal conditions, but might also provide an early advantage to HIV-1 and other sexually transmitted viruses... - Increased adhesion molecule and chemokine receptor expression on CD8+ T cells trafficking to cerebrospinal fluid in HIV-1 infectionBarbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, USA
J Infect Dis 189:2202-12. 2004..The central nervous system (CNS) is a recognized target for human immunodeficiency virus type 1 (HIV-1). CD8(+) T cells may mediate viral clearance from the CNS but also may contribute to immune-mediated neuronal damage... - Mucosal T-cell responses to HIV: responding at the front linesB L Shacklett
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA
J Intern Med 265:58-66. 2009..This review summarizes recent literature on HIV-specific T-cell responses in mucosal tissues, with an emphasis on the gastrointestinal tract... - Immune responses to HIV and SIV in mucosal tissues: 'location, location, location'Barbara L Shacklett
Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA
Curr Opin HIV AIDS 5:128-34. 2010..This review summarizes research literature regarding mucosal immunity to HIV and simian immunodeficiency virus (SIV), with an emphasis on work published within the past 18 months... - Mucosal immunity in HIV controllers: the right place at the right timeBarbara L Shacklett
Department of Medical Microbiology and Immunology, University of California at Davis, Davis, California, USA
Curr Opin HIV AIDS 6:202-7. 2011..This review focuses on studies of HIV-specific immune responses in mucosal tissues as a potential correlate of immune control, with an emphasis on recently published work... - Cell-mediated immunity to HIV in the female reproductive tractBarbara L Shacklett
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
J Reprod Immunol 83:190-5. 2009..This review provides an overview of recent findings on cell-mediated immunity to HIV in the female reproductive tract... 
Co-immunization with IL-15 enhances cellular immune responses induced by a vif-deleted simian immunodeficiency virus proviral DNA vaccine and confers partial protection against vaginal challenge with SIVmac251Robert A Dubie
Department of Medicine and Epidemiology, 2108 Tupper Hall, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
Virology 386:109-21. 2009..Taken together, these findings support the use of IL-15 as an adjuvant in prophylactic anti-HIV vaccine strategies...- Live, attenuated simian immunodeficiency virus SIVmac-M4, with point mutations in the Env transmembrane protein intracytoplasmic domain, provides partial protection from mucosal challenge with pathogenic SIVmac251Barbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco 94141 9100, USA
J Virol 76:11365-78. 2002..These findings demonstrate that SIVmac-M4, which contains a full-length Nef coding region and multiple point mutations in the TM, can provide substantial protection from mucosal challenge with pathogenic SIVmac251... - Viral suppression and immune restoration in the gastrointestinal mucosa of human immunodeficiency virus type 1-infected patients initiating therapy during primary or chronic infectionMoraima Guadalupe
Dept. of Medical Microbiology and Immunology, GBSF, Room 5511, University of California, Davis, CA 95616, USA
J Virol 80:8236-47. 2006.... - Magnitude and complexity of rectal mucosa HIV-1-specific CD8+ T-cell responses during chronic infection reflect clinical statusJ William Critchfield
Department of Medical Microbiology and Immunology, University of California Davis, Davis, California, USA
PLoS ONE 3:e3577. 2008..The ability of HIV-specific CD8+ T-cells to modulate disease course has prompted intensive study, yet the significance of virus-specific CD8+ T-cells in mucosal sites remains unclear... - Immunodominant HIV-specific CD8+ T-cell responses are common to blood and gastrointestinal mucosa, and Gag-specific responses dominate in rectal mucosa of HIV controllersApril L Ferre
Dept of Medical Microbiology and Immunology, University of California, Davis, CA 95616, USA
J Virol 84:10354-65. 2010.... - Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251Barbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California-San Francisco, San Francisco, California 94103, USA
AIDS Res Hum Retroviruses 18:1081-8. 2002..These results support a protective role for SIVmac-specific T cells in repeatedly exposed, persistently seronegative rhesus macaques... - HLA class I-restricted T-cell responses may contribute to the control of human immunodeficiency virus infection, but such responses are not always necessary for long-term virus controlBrinda Emu
Positive Health Program, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, California, USA
J Virol 82:5398-407. 2008..Defining mechanisms for virus control in "non-T-cell controllers" might lead to insights into preventing HIV transmission or preventing virus replication... - Mucosal immune responses to HIV-1 in elite controllers: a potential correlate of immune controlApril L Ferre
Department of Medical Microbiology and Immunology, University of California Davis, CA 95616, USA
Blood 113:3978-89. 2009..These responses may play an important role in mucosal immune surveillance, as suggested by their relative enrichment among persons who control HIV in the absence of therapy... - Vaccination of rhesus macaques with a vif-deleted simian immunodeficiency virus proviral DNA vaccineEllen E Sparger
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
Virology 374:261-72. 2008..Thus, a vif-deleted SIVmac239 proviral DNA vaccine is immunogenic and capable of inducing a transient suppression of pathogenic challenge virus, despite severe attenuation of the vaccine virus... - Isolating mucosal lymphocytes from biopsy tissue for cellular immunology assaysBarbara L Shacklett
Department of Medical Microbiology, and Division of Infectious Diseases, School of Medicine, University of California, Davis, CA, USA
Methods Mol Biol 485:347-56. 2009..These methods are also applicable, with minor modifications, to small tissue samples obtained from other lymphoid tissues... - Viral sanctuaries during highly active antiretroviral therapy in a nonhuman primate model for AIDSThomas W North
Center for Comparative Medicine, Department of Veterinary Molecular Biosciences, University of California, Davis, CA 95616, USA
J Virol 84:2913-22. 2010..This study is the first comprehensive analysis of the tissue and organ distribution of a primate AIDS virus during HAART. These data demonstrate widespread persistence of residual virus in tissues during HAART... - Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infectionJ William Critchfield
Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
J Virol 81:5460-71. 2007..These findings demonstrate that rectal CD8(+) T cells are capable of robust and varied HIV-1-specific responses and therefore likely play an active role in eliminating infected cells during chronic infection... - HIV controllers with HLA-DRB1*13 and HLA-DQB1*06 alleles have strong, polyfunctional mucosal CD4+ T-cell responsesApril L Ferre
Department of Medical Microbiology and Immunology, School of Medicine, University of California, 3327 Tupper Hall, 1 Shields Ave, Davis, CA 95616, USA
J Virol 84:11020-9. 2010..43, P=0.005), suggesting that increased CD4+ T-cell "help" may be important in maintaining strong CD8+ T-cell responses in the gut of HIV controllers... - Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infectionBarbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
J Virol 77:5621-31. 2003..These studies demonstrate the feasibility of using tetramers to assess HIV-specific T cells in GALT and reveal that GALT is the site of an active CD8(+) T-cell response during chronic infection... - Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15--the Amplispot assayWim Jennes
Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA, USA
J Immunol Methods 270:99-108. 2002..This method will be particularly useful for the assessment of antigen-stimulated cytokine production by T cells in cryopreserved biological specimens... - Optimization of methods to assess human mucosal T-cell responses to HIV infectionBarbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
J Immunol Methods 279:17-31. 2003..Taken together, these methods will be useful for studies of mucosal immunity to HIV-1 and other pathogens during natural infection and following vaccination... - Poorly soluble peptides can mimic authentic ELISPOT responsesR Karl Karlsson
Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
J Immunol Methods 285:89-92. 2004..Filtering the peptide prevented non-specific spot formation. These findings underscore the need for appropriate controls and proper peptide preparation in order to reduce the risk of false-positive ELISPOT responses... - Dendritic cell amplification of HIV type 1-specific CD8+ T cell responses in exposed, seronegative heterosexual womenBarbara L Shacklett
Gladstone Institute of Virology and Immunology, University of California San Francisco, PO Box 419100, San Francisco, CA 94110 9100, USA
AIDS Res Hum Retroviruses 18:805-15. 2002..This report also demonstrates the ability of dendritic cells to reveal T cell responses that might be overlooked by other methods... - Quantifying HIV-1-specific CD8 (+) T-cell responses using ELISPOT and cytokine flow cytometryBarbara L Shacklett
Department of Medical Microbiology, and Division of Infectious Diseases, School of Medicine, University of California, Davis, CA, USA
Methods Mol Biol 485:359-74. 2009..This chapter provides simple protocols for these two methods... - Seroconversion following nonoccupational postexposure prophylaxis against HIVMichelle E Roland
Positive Health Program at San Francisco General Hospital, California, USA
Clin Infect Dis 41:1507-13. 2005..We sought to describe the occurrence and circumstances of HIV seroconversion following nonoccupational PEP... - Increased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollersJulia M Shaw
Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, 1 Shields Ave, Davis, CA 95616, USA
J Virol 85:11422-34. 2011..Moreover, mucosal Treg maintain their suppressive ability during chronic HIV infection, potentially contributing to diminished HIV-specific T cell responses and viral persistence... - Perforin expression in the gastrointestinal mucosa is limited to acute simian immunodeficiency virus infectionMaire F Quigley
Center for Infection Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
J Virol 80:3083-7. 2006..These findings demonstrate the presence of a robust perforin-positive response in gastrointestinal CD8 T cells during acute, but not chronic, SIV infection... - Correlates of nontransmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposureJoan H Skurnick
Department of Preventive Medicine and Community Health, New Jersey Medical School, Newark, NJ 07103 2714, USA
J Infect Dis 185:428-38. 2002..These observations suggest that the primary protective factor is CD8 cell activity in both the HIV-positive donor and the HIV-negative partner. These findings have substantial implications for vaccine development... - Multidrug-resistant, dual-tropic HIV-1 and rapid progressionDouglas F Nixon
Lancet 365:1924-5. 2005
Research Grants
- Frequency and Function of HIV-specific T-cells in GALTBarbara Shacklett; Fiscal Year: 2003..We will also test alternate hypotheses to explain the apparent low levels of perforin expression in GALT. ..
- HIV-Specific T Cell Responses in Rectal MucosaBarbara L Shacklett; Fiscal Year: 2010..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..
- HIV-Specific T Cell Responses in Rectal MucosaBarbara Shacklett; Fiscal Year: 2009..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..
- Cell-mediated immune responses to HIV-1 in GALTBarbara Shacklett; Fiscal Year: 2007..We will also test alternate hypotheses to explain the apparent low levels of perforin expression in GALT. ..
- HIV-Specific T-cells in cerebrospinal fluid (CSF)Barbara Shacklett; Fiscal Year: 2003..We also hypothesize that there may be a correlation between intrathecal HIV viral load and T-cell recruitment to CSF, and that the presence of CTL in CSF may be predictive of neurological disease. ..
- HIV-Specific T Cell Responses in Rectal MucosaBarbara L Shacklett; Fiscal Year: 2010..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..