Barbara Shacklett

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Amplification of low-frequency antiviral CD8 T cell responses using autologous dendritic cells
    Marie Larsson
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY, USA
    AIDS 16:171-80. 2002
  2. ncbi request reprint Detection of macaque perforin expression and release by flow cytometry, immunohistochemistry, ELISA, and ELISpot
    Bartek Zuber
    Mabtech, Box 1233, SE 131 28 Nacka, Sweden
    J Immunol Methods 312:45-53. 2006
  3. pmc Can the new humanized mouse model give HIV research a boost
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, Division of Infectious Diseases, School of Medicine, University of California, Davis, Davis, California, United States of America
    PLoS Med 5:e13. 2008
  4. pmc Randomized pilot trial of a synbiotic dietary supplement in chronic HIV-1 infection
    Marco Schunter
    Department of Medical Microbiology and Immunology, University of California, Davis, CA, USA
    BMC Complement Altern Med 12:84. 2012
  5. ncbi request reprint Understanding the "lucky few": the conundrum of HIV-exposed, seronegative individuals
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Curr HIV/AIDS Rep 3:26-31. 2006
  6. pmc Cell-mediated immunity to HIV in the female reproductive tract
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
    J Reprod Immunol 83:190-5. 2009
  7. ncbi request reprint Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology GIVI, Department of Medicine, University of California San Francisco, San Francisco, CA 94141, USA
    J Immunol 173:641-8. 2004
  8. ncbi request reprint Increased adhesion molecule and chemokine receptor expression on CD8+ T cells trafficking to cerebrospinal fluid in HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, USA
    J Infect Dis 189:2202-12. 2004
  9. pmc Immune responses to HIV and SIV in mucosal tissues: 'location, location, location'
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Curr Opin HIV AIDS 5:128-34. 2010
  10. pmc Mucosal T-cell responses to HIV: responding at the front lines
    B L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA
    J Intern Med 265:58-66. 2009

Research Grants

  1. Frequency and Function of HIV-specific T-cells in GALT
    Barbara Shacklett; Fiscal Year: 2003
  2. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara L Shacklett; Fiscal Year: 2010
  3. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara Shacklett; Fiscal Year: 2009
  4. Cell-mediated immune responses to HIV-1 in GALT
    Barbara Shacklett; Fiscal Year: 2007
  5. HIV-Specific T-cells in cerebrospinal fluid (CSF)
    Barbara Shacklett; Fiscal Year: 2003
  6. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara L Shacklett; Fiscal Year: 2010

Collaborators

Detail Information

Publications36

  1. ncbi request reprint Amplification of low-frequency antiviral CD8 T cell responses using autologous dendritic cells
    Marie Larsson
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY, USA
    AIDS 16:171-80. 2002
    ..To utilize the potent antigen-presenting capacity of mature dendritic cells (MDC) in order to develop a rapid, sensitive method for quantifying antigen-specific CD8 T cells present at low frequency in peripheral blood...
  2. ncbi request reprint Detection of macaque perforin expression and release by flow cytometry, immunohistochemistry, ELISA, and ELISpot
    Bartek Zuber
    Mabtech, Box 1233, SE 131 28 Nacka, Sweden
    J Immunol Methods 312:45-53. 2006
    ..These macaque PFN-reactive mAbs and immunoassays will be valuable new tools for investigation of cytotoxic T lymphocyte (CTL) responses in non-human primate models of infectious diseases as well as for vaccine development...
  3. pmc Can the new humanized mouse model give HIV research a boost
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, Division of Infectious Diseases, School of Medicine, University of California, Davis, Davis, California, United States of America
    PLoS Med 5:e13. 2008
  4. pmc Randomized pilot trial of a synbiotic dietary supplement in chronic HIV-1 infection
    Marco Schunter
    Department of Medical Microbiology and Immunology, University of California, Davis, CA, USA
    BMC Complement Altern Med 12:84. 2012
    ..Known properties of pre-, pro-, and synbiotics suggest that they may be useful tools in achieving this goal...
  5. ncbi request reprint Understanding the "lucky few": the conundrum of HIV-exposed, seronegative individuals
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Curr HIV/AIDS Rep 3:26-31. 2006
    ..This review describes the major findings on this important topic, with a focus on immunologic studies...
  6. pmc Cell-mediated immunity to HIV in the female reproductive tract
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
    J Reprod Immunol 83:190-5. 2009
    ..This review provides an overview of recent findings on cell-mediated immunity to HIV in the female reproductive tract...
  7. ncbi request reprint Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology GIVI, Department of Medicine, University of California San Francisco, San Francisco, CA 94141, USA
    J Immunol 173:641-8. 2004
    ..Furthermore, the relative absence of armed effector cells may serve to protect the integrity of rectal mucosa under normal conditions, but might also provide an early advantage to HIV-1 and other sexually transmitted viruses...
  8. ncbi request reprint Increased adhesion molecule and chemokine receptor expression on CD8+ T cells trafficking to cerebrospinal fluid in HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, USA
    J Infect Dis 189:2202-12. 2004
    ..The central nervous system (CNS) is a recognized target for human immunodeficiency virus type 1 (HIV-1). CD8(+) T cells may mediate viral clearance from the CNS but also may contribute to immune-mediated neuronal damage...
  9. pmc Immune responses to HIV and SIV in mucosal tissues: 'location, location, location'
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Curr Opin HIV AIDS 5:128-34. 2010
    ..This review summarizes research literature regarding mucosal immunity to HIV and simian immunodeficiency virus (SIV), with an emphasis on work published within the past 18 months...
  10. pmc Mucosal T-cell responses to HIV: responding at the front lines
    B L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA
    J Intern Med 265:58-66. 2009
    ..This review summarizes recent literature on HIV-specific T-cell responses in mucosal tissues, with an emphasis on the gastrointestinal tract...
  11. pmc Mucosal immunity in HIV controllers: the right place at the right time
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, University of California at Davis, Davis, California, USA
    Curr Opin HIV AIDS 6:202-7. 2011
    ..This review focuses on studies of HIV-specific immune responses in mucosal tissues as a potential correlate of immune control, with an emphasis on recently published work...
  12. pmc Immune responses to HIV in the female reproductive tract, immunologic parallels with the gastrointestinal tract, and research implications
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, and Division of Infectious Diseases, Department of Internal Medicine, School of Medicine, University of California Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Am J Reprod Immunol 65:230-41. 2011
    ..The susceptibility of the female reproductive tract to HIV infection and immunopathogenesis suggests important new avenues for further research...
  13. pmc Live, attenuated simian immunodeficiency virus SIVmac-M4, with point mutations in the Env transmembrane protein intracytoplasmic domain, provides partial protection from mucosal challenge with pathogenic SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco 94141 9100, USA
    J Virol 76:11365-78. 2002
    ..These findings demonstrate that SIVmac-M4, which contains a full-length Nef coding region and multiple point mutations in the TM, can provide substantial protection from mucosal challenge with pathogenic SIVmac251...
  14. pmc Co-immunization with IL-15 enhances cellular immune responses induced by a vif-deleted simian immunodeficiency virus proviral DNA vaccine and confers partial protection against vaginal challenge with SIVmac251
    Robert A Dubie
    Department of Medicine and Epidemiology, 2108 Tupper Hall, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Virology 386:109-21. 2009
    ..Taken together, these findings support the use of IL-15 as an adjuvant in prophylactic anti-HIV vaccine strategies...
  15. pmc Viral suppression and immune restoration in the gastrointestinal mucosa of human immunodeficiency virus type 1-infected patients initiating therapy during primary or chronic infection
    Moraima Guadalupe
    Dept of Medical Microbiology and Immunology, GBSF, Room 5511, University of California, Davis, CA 95616, USA
    J Virol 80:8236-47. 2006
    ....
  16. pmc Magnitude and complexity of rectal mucosa HIV-1-specific CD8+ T-cell responses during chronic infection reflect clinical status
    J William Critchfield
    Department of Medical Microbiology and Immunology, University of California Davis, Davis, California, USA
    PLoS ONE 3:e3577. 2008
    ..The ability of HIV-specific CD8+ T-cells to modulate disease course has prompted intensive study, yet the significance of virus-specific CD8+ T-cells in mucosal sites remains unclear...
  17. pmc Immunodominant HIV-specific CD8+ T-cell responses are common to blood and gastrointestinal mucosa, and Gag-specific responses dominate in rectal mucosa of HIV controllers
    April L Ferre
    Dept of Medical Microbiology and Immunology, University of California, Davis, CA 95616, USA
    J Virol 84:10354-65. 2010
    ....
  18. ncbi request reprint Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California 94103, USA
    AIDS Res Hum Retroviruses 18:1081-8. 2002
    ..These results support a protective role for SIVmac-specific T cells in repeatedly exposed, persistently seronegative rhesus macaques...
  19. pmc HLA class I-restricted T-cell responses may contribute to the control of human immunodeficiency virus infection, but such responses are not always necessary for long-term virus control
    Brinda Emu
    Positive Health Program, Department of Medicine, San Francisco General Hospital, University of California, San Francisco, California, USA
    J Virol 82:5398-407. 2008
    ..Defining mechanisms for virus control in "non-T-cell controllers" might lead to insights into preventing HIV transmission or preventing virus replication...
  20. pmc Mucosal immune responses to HIV-1 in elite controllers: a potential correlate of immune control
    April L Ferre
    Department of Medical Microbiology and Immunology, University of California Davis, CA 95616, USA
    Blood 113:3978-89. 2009
    ..These responses may play an important role in mucosal immune surveillance, as suggested by their relative enrichment among persons who control HIV in the absence of therapy...
  21. pmc Vaccination of rhesus macaques with a vif-deleted simian immunodeficiency virus proviral DNA vaccine
    Ellen E Sparger
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Virology 374:261-72. 2008
    ..Thus, a vif-deleted SIVmac239 proviral DNA vaccine is immunogenic and capable of inducing a transient suppression of pathogenic challenge virus, despite severe attenuation of the vaccine virus...
  22. pmc Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infection
    J William Critchfield
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
    J Virol 81:5460-71. 2007
    ..These findings demonstrate that rectal CD8(+) T cells are capable of robust and varied HIV-1-specific responses and therefore likely play an active role in eliminating infected cells during chronic infection...
  23. pmc Viral sanctuaries during highly active antiretroviral therapy in a nonhuman primate model for AIDS
    Thomas W North
    Center for Comparative Medicine, Department of Veterinary Molecular Biosciences, University of California, Davis, CA 95616, USA
    J Virol 84:2913-22. 2010
    ..This study is the first comprehensive analysis of the tissue and organ distribution of a primate AIDS virus during HAART. These data demonstrate widespread persistence of residual virus in tissues during HAART...
  24. pmc Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
    J Virol 77:5621-31. 2003
    ..These studies demonstrate the feasibility of using tetramers to assess HIV-specific T cells in GALT and reveal that GALT is the site of an active CD8(+) T-cell response during chronic infection...
  25. pmc HIV controllers with HLA-DRB1*13 and HLA-DQB1*06 alleles have strong, polyfunctional mucosal CD4+ T-cell responses
    April L Ferre
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, 3327 Tupper Hall, 1 Shields Ave, Davis, CA 95616, USA
    J Virol 84:11020-9. 2010
    ..43, P=0.005), suggesting that increased CD4+ T-cell "help" may be important in maintaining strong CD8+ T-cell responses in the gut of HIV controllers...
  26. doi request reprint Isolating mucosal lymphocytes from biopsy tissue for cellular immunology assays
    Barbara L Shacklett
    Department of Medical Microbiology, and Division of Infectious Diseases, School of Medicine, University of California, Davis, CA, USA
    Methods Mol Biol 485:347-56. 2009
    ..These methods are also applicable, with minor modifications, to small tissue samples obtained from other lymphoid tissues...
  27. ncbi request reprint Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15--the Amplispot assay
    Wim Jennes
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA, USA
    J Immunol Methods 270:99-108. 2002
    ..This method will be particularly useful for the assessment of antigen-stimulated cytokine production by T cells in cryopreserved biological specimens...
  28. ncbi request reprint Optimization of methods to assess human mucosal T-cell responses to HIV infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    J Immunol Methods 279:17-31. 2003
    ..Taken together, these methods will be useful for studies of mucosal immunity to HIV-1 and other pathogens during natural infection and following vaccination...
  29. doi request reprint Quantifying HIV-1-specific CD8 (+) T-cell responses using ELISPOT and cytokine flow cytometry
    Barbara L Shacklett
    Department of Medical Microbiology, and Division of Infectious Diseases, School of Medicine, University of California, Davis, CA, USA
    Methods Mol Biol 485:359-74. 2009
    ..This chapter provides simple protocols for these two methods...
  30. ncbi request reprint Dendritic cell amplification of HIV type 1-specific CD8+ T cell responses in exposed, seronegative heterosexual women
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, PO Box 419100, San Francisco, CA 94110 9100, USA
    AIDS Res Hum Retroviruses 18:805-15. 2002
    ..This report also demonstrates the ability of dendritic cells to reveal T cell responses that might be overlooked by other methods...
  31. ncbi request reprint Poorly soluble peptides can mimic authentic ELISPOT responses
    R Karl Karlsson
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol Methods 285:89-92. 2004
    ..Filtering the peptide prevented non-specific spot formation. These findings underscore the need for appropriate controls and proper peptide preparation in order to reduce the risk of false-positive ELISPOT responses...
  32. ncbi request reprint Seroconversion following nonoccupational postexposure prophylaxis against HIV
    Michelle E Roland
    Positive Health Program at San Francisco General Hospital, California, USA
    Clin Infect Dis 41:1507-13. 2005
    ..We sought to describe the occurrence and circumstances of HIV seroconversion following nonoccupational PEP...
  33. pmc Increased frequency of regulatory T cells accompanies increased immune activation in rectal mucosae of HIV-positive noncontrollers
    Julia M Shaw
    Department of Medical Microbiology and Immunology, School of Medicine, University of California Davis, 1 Shields Ave, Davis, CA 95616, USA
    J Virol 85:11422-34. 2011
    ..Moreover, mucosal Treg maintain their suppressive ability during chronic HIV infection, potentially contributing to diminished HIV-specific T cell responses and viral persistence...
  34. pmc Perforin expression in the gastrointestinal mucosa is limited to acute simian immunodeficiency virus infection
    Maire F Quigley
    Center for Infection Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    J Virol 80:3083-7. 2006
    ..These findings demonstrate the presence of a robust perforin-positive response in gastrointestinal CD8 T cells during acute, but not chronic, SIV infection...
  35. pmc Correlates of nontransmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposure
    Joan H Skurnick
    Department of Preventive Medicine and Community Health, New Jersey Medical School, Newark, NJ 07103 2714, USA
    J Infect Dis 185:428-38. 2002
    ..These observations suggest that the primary protective factor is CD8 cell activity in both the HIV-positive donor and the HIV-negative partner. These findings have substantial implications for vaccine development...
  36. ncbi request reprint Multidrug-resistant, dual-tropic HIV-1 and rapid progression
    Douglas F Nixon
    Lancet 365:1924-5. 2005

Research Grants11

  1. Frequency and Function of HIV-specific T-cells in GALT
    Barbara Shacklett; Fiscal Year: 2003
    ..We will also test alternate hypotheses to explain the apparent low levels of perforin expression in GALT. ..
  2. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara L Shacklett; Fiscal Year: 2010
    ..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..
  3. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara Shacklett; Fiscal Year: 2009
    ..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..
  4. Cell-mediated immune responses to HIV-1 in GALT
    Barbara Shacklett; Fiscal Year: 2007
    ..We will also test alternate hypotheses to explain the apparent low levels of perforin expression in GALT. ..
  5. HIV-Specific T-cells in cerebrospinal fluid (CSF)
    Barbara Shacklett; Fiscal Year: 2003
    ..We also hypothesize that there may be a correlation between intrathecal HIV viral load and T-cell recruitment to CSF, and that the presence of CTL in CSF may be predictive of neurological disease. ..
  6. HIV-Specific T Cell Responses in Rectal Mucosa
    Barbara L Shacklett; Fiscal Year: 2010
    ..Taken together, these studies should greatly advance our understanding of the HIV-host relationship in mucosal tissues during chronic infection. ..