B L Shacklett

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Dendritic cell amplification of HIV type 1-specific CD8+ T cell responses in exposed, seronegative heterosexual women
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, PO Box 419100, San Francisco, CA 94110 9100, USA
    AIDS Res Hum Retroviruses 18:805-15. 2002
  2. pmc Live, attenuated simian immunodeficiency virus SIVmac-M4, with point mutations in the Env transmembrane protein intracytoplasmic domain, provides partial protection from mucosal challenge with pathogenic SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco 94141 9100, USA
    J Virol 76:11365-78. 2002
  3. pmc Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues
    B L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, 94103, USA
    Clin Exp Immunol 130:172-82. 2002
  4. pmc Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
    J Virol 77:5621-31. 2003
  5. pmc Mucosal T-cell responses to HIV: responding at the front lines
    B L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA
    J Intern Med 265:58-66. 2009
  6. ncbi request reprint Limited magnitude and breadth in the HLA-A2-restricted CD8 T-Cell response to Nef in children with vertically acquired HIV-1 infection
    R Chandwani
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    Scand J Immunol 59:109-14. 2004
  7. pmc Perforin expression in the gastrointestinal mucosa is limited to acute simian immunodeficiency virus infection
    Maire F Quigley
    Center for Infection Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    J Virol 80:3083-7. 2006
  8. ncbi request reprint Detection of macaque perforin expression and release by flow cytometry, immunohistochemistry, ELISA, and ELISpot
    Bartek Zuber
    Mabtech, Box 1233, SE 131 28 Nacka, Sweden
    J Immunol Methods 312:45-53. 2006
  9. pmc Viral suppression and immune restoration in the gastrointestinal mucosa of human immunodeficiency virus type 1-infected patients initiating therapy during primary or chronic infection
    Moraima Guadalupe
    Dept of Medical Microbiology and Immunology, GBSF, Room 5511, University of California, Davis, CA 95616, USA
    J Virol 80:8236-47. 2006
  10. pmc Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infection
    J William Critchfield
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
    J Virol 81:5460-71. 2007

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Dendritic cell amplification of HIV type 1-specific CD8+ T cell responses in exposed, seronegative heterosexual women
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, PO Box 419100, San Francisco, CA 94110 9100, USA
    AIDS Res Hum Retroviruses 18:805-15. 2002
    ..This report also demonstrates the ability of dendritic cells to reveal T cell responses that might be overlooked by other methods...
  2. pmc Live, attenuated simian immunodeficiency virus SIVmac-M4, with point mutations in the Env transmembrane protein intracytoplasmic domain, provides partial protection from mucosal challenge with pathogenic SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco 94141 9100, USA
    J Virol 76:11365-78. 2002
    ..These findings demonstrate that SIVmac-M4, which contains a full-length Nef coding region and multiple point mutations in the TM, can provide substantial protection from mucosal challenge with pathogenic SIVmac251...
  3. pmc Beyond 51Cr release: New methods for assessing HIV-1-specific CD8+ T cell responses in peripheral blood and mucosal tissues
    B L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, 94103, USA
    Clin Exp Immunol 130:172-82. 2002
    ..This review focuses on recent developments in the assessment of HIV-1-specific T cell responses in peripheral blood and tissues, with a particular emphasis on flow cytometry-based approaches...
  4. pmc Trafficking of human immunodeficiency virus type 1-specific CD8+ T cells to gut-associated lymphoid tissue during chronic infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California 94141 9100, USA
    J Virol 77:5621-31. 2003
    ..These studies demonstrate the feasibility of using tetramers to assess HIV-specific T cells in GALT and reveal that GALT is the site of an active CD8(+) T-cell response during chronic infection...
  5. pmc Mucosal T-cell responses to HIV: responding at the front lines
    B L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA, USA
    J Intern Med 265:58-66. 2009
    ..This review summarizes recent literature on HIV-specific T-cell responses in mucosal tissues, with an emphasis on the gastrointestinal tract...
  6. ncbi request reprint Limited magnitude and breadth in the HLA-A2-restricted CD8 T-Cell response to Nef in children with vertically acquired HIV-1 infection
    R Chandwani
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    Scand J Immunol 59:109-14. 2004
    ..These findings indicate significant weakness in strength and breadth of the CD8 T-cell response to the target protein Nef in infected children and prompt renewed efforts into the immunology of vertically acquired HIV-1 infection...
  7. pmc Perforin expression in the gastrointestinal mucosa is limited to acute simian immunodeficiency virus infection
    Maire F Quigley
    Center for Infection Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
    J Virol 80:3083-7. 2006
    ..These findings demonstrate the presence of a robust perforin-positive response in gastrointestinal CD8 T cells during acute, but not chronic, SIV infection...
  8. ncbi request reprint Detection of macaque perforin expression and release by flow cytometry, immunohistochemistry, ELISA, and ELISpot
    Bartek Zuber
    Mabtech, Box 1233, SE 131 28 Nacka, Sweden
    J Immunol Methods 312:45-53. 2006
    ..These macaque PFN-reactive mAbs and immunoassays will be valuable new tools for investigation of cytotoxic T lymphocyte (CTL) responses in non-human primate models of infectious diseases as well as for vaccine development...
  9. pmc Viral suppression and immune restoration in the gastrointestinal mucosa of human immunodeficiency virus type 1-infected patients initiating therapy during primary or chronic infection
    Moraima Guadalupe
    Dept of Medical Microbiology and Immunology, GBSF, Room 5511, University of California, Davis, CA 95616, USA
    J Virol 80:8236-47. 2006
    ....
  10. pmc Multifunctional human immunodeficiency virus (HIV) gag-specific CD8+ T-cell responses in rectal mucosa and peripheral blood mononuclear cells during chronic HIV type 1 infection
    J William Critchfield
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, CA 95616, USA
    J Virol 81:5460-71. 2007
    ..These findings demonstrate that rectal CD8(+) T cells are capable of robust and varied HIV-1-specific responses and therefore likely play an active role in eliminating infected cells during chronic infection...
  11. pmc Can the new humanized mouse model give HIV research a boost
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, Division of Infectious Diseases, School of Medicine, University of California, Davis, Davis, California, United States of America
    PLoS Med 5:e13. 2008
  12. pmc Vaccination of rhesus macaques with a vif-deleted simian immunodeficiency virus proviral DNA vaccine
    Ellen E Sparger
    Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA
    Virology 374:261-72. 2008
    ..Thus, a vif-deleted SIVmac239 proviral DNA vaccine is immunogenic and capable of inducing a transient suppression of pathogenic challenge virus, despite severe attenuation of the vaccine virus...
  13. ncbi request reprint Seroconversion following nonoccupational postexposure prophylaxis against HIV
    Michelle E Roland
    Positive Health Program at San Francisco General Hospital, California, USA
    Clin Infect Dis 41:1507-13. 2005
    ..We sought to describe the occurrence and circumstances of HIV seroconversion following nonoccupational PEP...
  14. ncbi request reprint Amplification of low-frequency antiviral CD8 T cell responses using autologous dendritic cells
    Marie Larsson
    Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, NY, USA
    AIDS 16:171-80. 2002
    ..To utilize the potent antigen-presenting capacity of mature dendritic cells (MDC) in order to develop a rapid, sensitive method for quantifying antigen-specific CD8 T cells present at low frequency in peripheral blood...
  15. pmc Correlates of nontransmission in US women at high risk of human immunodeficiency virus type 1 infection through sexual exposure
    Joan H Skurnick
    Department of Preventive Medicine and Community Health, New Jersey Medical School, Newark, NJ 07103 2714, USA
    J Infect Dis 185:428-38. 2002
    ..These observations suggest that the primary protective factor is CD8 cell activity in both the HIV-positive donor and the HIV-negative partner. These findings have substantial implications for vaccine development...
  16. ncbi request reprint Enhanced ELISPOT detection of antigen-specific T cell responses from cryopreserved specimens with addition of both IL-7 and IL-15--the Amplispot assay
    Wim Jennes
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, CA, USA
    J Immunol Methods 270:99-108. 2002
    ..This method will be particularly useful for the assessment of antigen-stimulated cytokine production by T cells in cryopreserved biological specimens...
  17. ncbi request reprint Boosting of SIV-specific T cell responses in rhesus macaques that resist repeated intravaginal challenge with SIVmac251
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California San Francisco, San Francisco, California 94103, USA
    AIDS Res Hum Retroviruses 18:1081-8. 2002
    ..These results support a protective role for SIVmac-specific T cells in repeatedly exposed, persistently seronegative rhesus macaques...
  18. ncbi request reprint Optimization of methods to assess human mucosal T-cell responses to HIV infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA, USA
    J Immunol Methods 279:17-31. 2003
    ..Taken together, these methods will be useful for studies of mucosal immunity to HIV-1 and other pathogens during natural infection and following vaccination...
  19. ncbi request reprint Poorly soluble peptides can mimic authentic ELISPOT responses
    R Karl Karlsson
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, CA 94141, USA
    J Immunol Methods 285:89-92. 2004
    ..Filtering the peptide prevented non-specific spot formation. These findings underscore the need for appropriate controls and proper peptide preparation in order to reduce the risk of false-positive ELISPOT responses...
  20. ncbi request reprint Increased adhesion molecule and chemokine receptor expression on CD8+ T cells trafficking to cerebrospinal fluid in HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology, University of California, San Francisco, California, USA
    J Infect Dis 189:2202-12. 2004
    ..The central nervous system (CNS) is a recognized target for human immunodeficiency virus type 1 (HIV-1). CD8(+) T cells may mediate viral clearance from the CNS but also may contribute to immune-mediated neuronal damage...
  21. ncbi request reprint Abundant expression of granzyme A, but not perforin, in granules of CD8+ T cells in GALT: implications for immune control of HIV-1 infection
    Barbara L Shacklett
    Gladstone Institute of Virology and Immunology GIVI, Department of Medicine, University of California San Francisco, San Francisco, CA 94141, USA
    J Immunol 173:641-8. 2004
    ..Furthermore, the relative absence of armed effector cells may serve to protect the integrity of rectal mucosa under normal conditions, but might also provide an early advantage to HIV-1 and other sexually transmitted viruses...
  22. ncbi request reprint Understanding the "lucky few": the conundrum of HIV-exposed, seronegative individuals
    Barbara L Shacklett
    Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
    Curr HIV/AIDS Rep 3:26-31. 2006
    ..This review describes the major findings on this important topic, with a focus on immunologic studies...
  23. doi request reprint Nine-color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part II: Panel performance across different instrument platforms
    Bridget E McLaughlin
    University of California Davis, Internal Medicine, Infectious Diseases, Davis, CA, USA
    Cytometry A 73:411-20. 2008
    ....
  24. ncbi request reprint Multidrug-resistant, dual-tropic HIV-1 and rapid progression
    Douglas F Nixon
    Lancet 365:1924-5. 2005