Peter Setlow

Summary

Affiliation: University of Connecticut Health Center
Country: USA

Publications

  1. ncbi request reprint I will survive: DNA protection in bacterial spores
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Trends Microbiol 15:172-80. 2007
  2. pmc Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores
    Arturo Ramirez-Peralta
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    Appl Environ Microbiol 78:2689-97. 2012
  3. doi request reprint Dynamics of the assembly of a complex macromolecular structure--the coat of spores of the bacterium Bacillus subtilis
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Microbiol 83:241-4. 2012
  4. pmc Investigating the role of small, acid-soluble spore proteins (SASPs) in the resistance of Clostridium perfringens spores to heat
    Deepa Raju
    Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 97331, USA
    BMC Microbiol 6:50. 2006
  5. ncbi request reprint Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals
    P Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    J Appl Microbiol 101:514-25. 2006
  6. ncbi request reprint Characterization of the germination of Bacillus megaterium spores lacking enzymes that degrade the spore cortex
    B Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Appl Microbiol 107:318-28. 2009
  7. doi request reprint Dormant spores receive an unexpected wake-up call
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Cell 135:410-2. 2008
  8. pmc Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination
    Pengfei Zhang
    Department of Physics, East Carolina University, Greenville, North Carolina, USA
    J Bacteriol 194:646-52. 2012
  9. ncbi request reprint Germination of spores of Bacillus subtilis with dodecylamine
    B Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 95:637-48. 2003
  10. ncbi request reprint Spore germination
    Peter Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Curr Opin Microbiol 6:550-6. 2003

Collaborators

Detail Information

Publications154 found, 100 shown here

  1. ncbi request reprint I will survive: DNA protection in bacterial spores
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Trends Microbiol 15:172-80. 2007
    ..The structure of both DNA and SASP alters upon their association and this causes major changes in the chemical and photochemical reactivity of DNA...
  2. pmc Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores
    Arturo Ramirez-Peralta
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    Appl Environ Microbiol 78:2689-97. 2012
    ....
  3. doi request reprint Dynamics of the assembly of a complex macromolecular structure--the coat of spores of the bacterium Bacillus subtilis
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Microbiol 83:241-4. 2012
    ..This study provides new understanding of the mechanism of the assembly of a multi-protein, multi-lamellar structure...
  4. pmc Investigating the role of small, acid-soluble spore proteins (SASPs) in the resistance of Clostridium perfringens spores to heat
    Deepa Raju
    Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon 97331, USA
    BMC Microbiol 6:50. 2006
    ..perfringens spore heat-resistance remains unknown. In the current study, we investigated the role of small, acid-soluble spore proteins (SASPs) in heat-resistance of spores produced by C. perfringens food poisoning isolates...
  5. ncbi request reprint Spores of Bacillus subtilis: their resistance to and killing by radiation, heat and chemicals
    P Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06030 3305, USA
    J Appl Microbiol 101:514-25. 2006
    ..Given the importance of the killing of spores of Bacillus species in the food and medical products industry, a deeper understanding of the mechanisms of spore resistance and killing may lead to improved methods for spore destruction...
  6. ncbi request reprint Characterization of the germination of Bacillus megaterium spores lacking enzymes that degrade the spore cortex
    B Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Appl Microbiol 107:318-28. 2009
    ..To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination...
  7. doi request reprint Dormant spores receive an unexpected wake-up call
    Peter Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Cell 135:410-2. 2008
    ..Shah et al. (2008) now report that cell wall peptidoglycan fragments can also trigger spore germination by binding to an inner membrane-bound protein kinase...
  8. pmc Effects of cortex peptidoglycan structure and cortex hydrolysis on the kinetics of Ca(2+)-dipicolinic acid release during Bacillus subtilis spore germination
    Pengfei Zhang
    Department of Physics, East Carolina University, Greenville, North Carolina, USA
    J Bacteriol 194:646-52. 2012
    ..subtilis spore germination and, further, that this effect is independent of effects of GRs...
  9. ncbi request reprint Germination of spores of Bacillus subtilis with dodecylamine
    B Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 95:637-48. 2003
    ..To determine the properties of Bacillus subtilis spores germinated with the alkylamine dodecylamine, and the mechanism of dodecylamine-induced spore germination...
  10. ncbi request reprint Spore germination
    Peter Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Curr Opin Microbiol 6:550-6. 2003
    ..subtilis, and completion of cortex hydrolysis and subsequent germ cell wall expansion allows full spore core hydration and resumption of spore metabolism and macromolecular synthesis...
  11. pmc Interaction between individual protein components of the GerA and GerB nutrient receptors that trigger germination of Bacillus subtilis spores
    Takao Igarashi
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 187:2513-8. 2005
    ..These findings suggest that GerAC and GerBC interact with their respective A and B proteins in GerA or GerB receptors and that GerBC potentially interacts with GerAA-GerAB. However, GerAC did not appear to interact with GerBA-GerBB...
  12. pmc Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins
    Barbara Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 188:3740-7. 2006
    ..Specific roles for DPA in protecting spore DNA against damage may well have been a major driving force for the spore's accumulation of the high levels of this small molecule...
  13. pmc Characterization of spores of Bacillus subtilis that lack most coat layers
    Sonali Ghosh
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 190:6741-8. 2008
    ..These results provide new insight into the role of the coat layer in spore properties...
  14. pmc Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination
    Yunfeng Li
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 195:2530-40. 2013
    ....
  15. doi request reprint Levels and localization of mechanosensitive channel proteins in Bacillus subtilis
    Paul G Wahome
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    Arch Microbiol 191:403-14. 2009
    ....
  16. pmc Superdormant spores of Bacillus species have elevated wet-heat resistance and temperature requirements for heat activation
    Sonali Ghosh
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Bacteriol 191:5584-91. 2009
    ..These results provide new information about the germination, heat activation optima, and wet-heat resistance of superdormant spores and the heterogeneity in these properties between individual members of dormant spore populations...
  17. ncbi request reprint Structural and Functional Analysis of the GerD Spore Germination Protein of Bacillus Species
    Yunfeng Li
    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Mol Biol 426:1995-2008. 2014
    ..subtilis. These results and those of others lead us to propose that GerD mediates clustering of germination proteins in the inner membrane of dormant spores and thus promotes the rapid and cooperative germination response to nutrients. ..
  18. ncbi request reprint Function of the SpoVAEa and SpoVAF proteins of Bacillus subtilis spores
    Abigail Pérez-Valdespino
    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 196:2077-88. 2014
    ....
  19. pmc Role of dipicolinic acid in the germination, stability, and viability of spores of Bacillus subtilis
    Anil Magge
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 190:4798-807. 2008
    ..vii) At least the great majority of spores of several spoVF strains that contained no DPA also lacked other major spore small molecules and had gone through some of the early reactions in spore germination...
  20. pmc Analysis of the effects of a gerP mutation on the germination of spores of Bacillus subtilis
    Xuan Yi Butzin
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:5749-58. 2012
    ..All these results support the suggestion that GerP proteins facilitate access of nutrient germinants to their cognate GRs in spores' inner membrane...
  21. pmc Role of a SpoVA protein in dipicolinic acid uptake into developing spores of Bacillus subtilis
    Yunfeng Li
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:1875-84. 2012
    ..Our results provide direct evidence that SpoVA proteins, in particular SpoVAD, are directly involved in DPA(2,6) movement into developing B. subtilis spores...
  22. pmc Effects of the SpoVT regulatory protein on the germination and germination protein levels of spores of Bacillus subtilis
    Arturo Ramirez-Peralta
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:3417-25. 2012
    ..Overall, these results indicate that SpoVT levels during spore formation have a major impact on the germination and the resistance of the resultant spores...
  23. pmc Effects of a gerF (lgt) mutation on the germination of spores of Bacillus subtilis
    Takao Igarashi
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 186:2984-91. 2004
    ....
  24. ncbi request reprint Mechanisms of Bacillus subtilis spore resistance to and killing by aqueous ozone
    S B Young
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    J Appl Microbiol 96:1133-42. 2004
    ..To determine the mechanisms of Bacillus subtilis spore killing by and resistance to aqueous ozone...
  25. pmc Role of SpoVA proteins in release of dipicolinic acid during germination of Bacillus subtilis spores triggered by dodecylamine or lysozyme
    Venkata Ramana Vepachedu
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 189:1565-72. 2007
    ....
  26. pmc Release of small molecules during germination of spores of Bacillus Species
    Barbara Setlow
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 190:4759-63. 2008
    ..These results indicate that early in germination there is a major selective change in the permeability of the spore's inner membrane...
  27. pmc Factors influencing germination of Bacillus subtilis spores via activation of nutrient receptors by high pressure
    Elaine P Black
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Appl Environ Microbiol 71:5879-87. 2005
    ....
  28. ncbi request reprint Mechanisms of Bacillus subtilis spore killing by and resistance to an acidic Fe-EDTA-iodide-ethanol formulation
    M P Shapiro
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, 06032, USA
    J Appl Microbiol 100:746-53. 2006
    ..To determine the mechanisms of Bacillus subtilis spore killing by and resistance to an acidic solution containing Fe(3+), EDTA, KI and ethanol termed the KMT reagent...
  29. ncbi request reprint Comparison of the properties of Bacillus subtilis spores made in liquid or on agar plates
    R Rose
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Appl Microbiol 103:691-9. 2007
    ..To compare the properties of the spores of Bacillus subtilis prepared in liquid and on plates...
  30. ncbi request reprint Mechanisms of killing of Bacillus subtilis spores by Decon and Oxone, two general decontaminants for biological agents
    S B Young
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    J Appl Microbiol 96:289-301. 2004
    ..To determine the mechanisms of Bacillus subtilis spore killing by and resistance to the general biological decontamination agents, Decon and Oxone...
  31. pmc Identification of new proteins that modulate the germination of spores of bacillus species
    Arturo Ramirez-Peralta
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 195:3009-21. 2013
    ..subtilis gerKD [gerKDbs]) adjacent to the gerK operon encoding the GerK GR or ectopic expression or overexpression of gerKDbs had no major effect on the levels of GR subunits or of two other germination proteins...
  32. pmc Transcription of the Bacillus subtilis gerK operon, which encodes a spore germinant receptor, and comparison with that of operons encoding other germinant receptors
    Takao Igarashi
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 188:4131-6. 2006
    ..The gerK promoter showed sequence similarities to sigma(G)-dependent promoters, and deletion of elements of this putative promoter abolished gerK expression in sporulation...
  33. pmc Crystal structure of the catalytic domain of the Bacillus cereus SleB protein, important in cortex peptidoglycan degradation during spore germination
    Yunfeng Li
    Department of Molecular, Microbial and Structural Biology University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:4537-45. 2012
    ..SleB and CwlJ may offer novel targets for the development of anti-spore agents...
  34. ncbi request reprint Analysis of the germination of spores of Bacillus subtilis with temperature sensitive spo mutations in the spoVA operon
    Venkata Ramana Vepachedu
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06032, USA
    FEMS Microbiol Lett 239:71-7. 2004
    ..These findings are discussed in light of the proposal that SpoVA proteins are involved not only in DPA uptake during sporulation, but also in DPA release during nutrient-mediated spore germination...
  35. pmc Superdormant spores of bacillus species germinate normally with high pressure, peptidoglycan fragments, and bryostatin
    Jie Wei
    Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 192:1455-8. 2010
    ..Superdormant B. subtilis spores also germinated as well as dormant spores with peptidoglycan fragments or bryostatin, a Ser/Thr protein kinase activator...
  36. pmc Localization of SpoVAD to the inner membrane of spores of Bacillus subtilis
    Venkata Ramana Vepachedu
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Bacteriol 187:5677-82. 2005
    ..SpoVAD also persisted in outgrowing spores...
  37. pmc Maturation of released spores is necessary for acquisition of full spore heat resistance during Bacillus subtilis sporulation
    Jose Luis Sanchez-Salas
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Appl Environ Microbiol 77:6746-54. 2011
    ..Nonetheless, it appears to be most likely that it is changes in coat structure that are responsible for the increased resistance to wet heat and hypochlorite upon early spore maturation...
  38. pmc Levels of glycine betaine in growing cells and spores of Bacillus species and lack of effect of glycine betaine on dormant spore resistance
    Charles A Loshon
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 188:3153-8. 2006
    ..GB levels were similar in wild-type B. subtilis spores and spores that lacked major small, acid-soluble spore proteins but were much lower in spores that lacked dipicolinic acid...
  39. pmc A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy
    Ann E Cowan
    Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Proc Natl Acad Sci U S A 100:4209-14. 2003
    ....
  40. pmc Cooperativity between different nutrient receptors in germination of spores of Bacillus subtilis and reduction of this cooperativity by alterations in the GerB receptor
    Swaroopa Atluri
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 188:28-36. 2006
    ..vii) The amino acid binding sites in GerA and GerB are different, even though both respond to L-alanine. These new conclusions are integrated into models for the signal transduction pathways that initiate spore germination...
  41. pmc Ultraviolet irradiation of DNA complexed with alpha/beta-type small, acid-soluble proteins from spores of Bacillus or Clostridium species makes spore photoproduct but not thymine dimers
    W L Nicholson
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 88:8288-92. 1991
    ..It is suggested that as these factors diffuse out in the first minutes of spore germination, spore photoproduct yields become similar to those observed for irradiation of SASP/DNA complexes in vitro...
  42. pmc Expression level of Bacillus subtilis germinant receptors determines the average rate but not the heterogeneity of spore germination
    Jing Qiao Zhang
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT, USA
    J Bacteriol 195:1735-40. 2013
    ..Our results indicated that the variation in the expression levels of GRs was not the primary factor that controls spore germination heterogeneity. Two alternative hypotheses are discussed in light of this experimental discovery...
  43. pmc Structure-based functional studies of the effects of amino acid substitutions in GerBC, the C subunit of the Bacillus subtilis GerB spore germinant receptor
    Yunfeng Li
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 193:4143-52. 2011
    ....
  44. pmc Effects of overexpression of nutrient receptors on germination of spores of Bacillus subtilis
    Rosa Martha Cabrera-Martinez
    Department of Biochemistry University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:2457-64. 2003
    ....
  45. pmc Effects of carboxy-terminal modifications and pH on binding of a Bacillus subtilis small, acid-soluble spore protein to DNA
    Jeffrey Kosman
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:6095-103. 2003
    ....
  46. pmc Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile
    Ann E Cowan
    Department of Molecular, Microbial, and Structural Biology and Center for Biomedical Imaging Technology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Proc Natl Acad Sci U S A 101:7733-8. 2004
    ..3- to 1.6-fold) apparent reduction of the membrane surface area in the dormant spore relative to that in the germinated spore, but is not due to the dormant spore's high levels of dipicolinic acid and divalent cations...
  47. ncbi request reprint Growth, osmotic downshock resistance and differentiation of Bacillus subtilis strains lacking mechanosensitive channels
    Paul G Wahome
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    Arch Microbiol 189:49-58. 2008
    ..Sporulation of all MS channel mutant strains was normal, and the mutant spores germinated normally with L-alanine or dodecylamine...
  48. pmc Killing of Bacillus subtilis spores by a modified Fenton reagent containing CuCl2 and ascorbic acid
    Michael P Shapiro
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    Appl Environ Microbiol 70:2535-9. 2004
    ..CuCl(2)-ascorbic acid did not damage spore DNA, and spores killed by this reagent initiated germination. However, spores killed by CuCl(2)-ascorbic acid may have damage to their inner membrane...
  49. pmc Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ
    Alicia Monroe
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, 06030, USA
    J Bacteriol 188:7609-16. 2006
    ..However, while the presence of any of these three lysine residues is essential for GerQ cross-linking, they are not essential for the function of GerQ in CwlJ localization...
  50. pmc Role of GerD in germination of Bacillus subtilis spores
    Patricia L Pelczar
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 189:1090-8. 2007
    ....
  51. pmc Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate
    Katerina Ragkousi
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 185:2315-29. 2003
    ..However, other than lacking CwlJ, ywdL spores have no obvious defect in their spore coat. Because of the role for YwdL in a part of the spore germination process, we propose renaming ywdL as a spore germination gene, gerQ...
  52. ncbi request reprint Germination proteins in the inner membrane of dormant Bacillus subtilis spores colocalize in a discrete cluster
    Keren K Griffiths
    Department of Molecular, Microbial and Structural Biology Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Mol Microbiol 81:1061-77. 2011
    ....
  53. pmc Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores
    Katerina Ragkousi
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Bacteriol 186:5567-75. 2004
    ....
  54. pmc An alpha/beta-type, small, acid-soluble spore protein which has very high affinity for DNA prevents outgrowth of Bacillus subtilis spores
    C S Hayes
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Bacteriol 183:2662-6. 2001
    ..The persistence of this protein on spore DNA then interferes with transcription during spore outgrowth...
  55. ncbi request reprint Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis
    Barbara Setlow
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Arch Microbiol 181:60-7. 2004
    ..However, a strain lacking bglA, bglH and yckE grew poorly if at all on aryl-beta- d-glucosides as the sole carbon source...
  56. pmc Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane
    George Korza
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT, USA
    J Bacteriol 195:1484-91. 2013
    ....
  57. pmc Efficient inhibition of germination of coat-deficient bacterial spores by multivalent metal cations, including terbium (Tb³+)
    Xuan Yi
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Appl Environ Microbiol 77:5536-9. 2011
    ..However, germination of coat-deficient Bacillus spores was strongly inhibited by Tb(3+) and some other multivalent cations. Tb(3+) also inhibited germination of coat-deficient Clostridium perfringens spores...
  58. ncbi request reprint Killing of spores of Bacillus subtilis by peroxynitrite appears to be caused by membrane damage
    Peter C Genest
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Microbiology 148:307-14. 2002
    ..Together these data suggest that spore killing by PON is through some type of damage to the spore's inner membrane...
  59. ncbi request reprint Analysis of interactions between nutrient germinant receptors and SpoVA proteins of Bacillus subtilis spores
    Venkata Ramana Vepachedu
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    FEMS Microbiol Lett 274:42-7. 2007
    ..Consequently, these new findings suggest that nutrient germinant receptors physically contact SpoVA proteins, and presumably this is a route for signal transduction during spore germination...
  60. pmc Effects of Mn and Fe levels on Bacillus subtilis spore resistance and effects of Mn2+, other divalent cations, orthophosphate, and dipicolinic acid on protein resistance to ionizing radiation
    Amanda C Granger
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Appl Environ Microbiol 77:32-40. 2011
    ....
  61. pmc Studies of the commitment step in the germination of spores of bacillus species
    Xuan Yi
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 192:3424-33. 2010
    ....
  62. ncbi request reprint Base-change mutations induced by various treatments of Bacillus subtilis spores with and without DNA protective small, acid-soluble spore proteins
    Luz del Carmen Huesca Espitia
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Mutat Res 503:77-84. 2002
    ....
  63. ncbi request reprint The synthesis and role of the mechanosensitive channel of large conductance in growth and differentiation of Bacillus subtilis
    Paul G Wahome
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030 3305, USA
    Arch Microbiol 186:377-83. 2006
    ..However, by the early stationary phase of growth, DeltamscL cells had become resistant to a 0.9 M osmotic downshift...
  64. pmc Localization of the germination protein GerD to the inner membrane in Bacillus subtilis spores
    Patricia L Pelczar
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 190:5635-41. 2008
    ..All of these results suggest that GerD is a lipoprotein associated with the dormant spore's inner membrane that may be released in some fashion from this membrane upon spore germination...
  65. pmc The Bacillus subtilis spore coat provides "eat resistance" during phagocytic predation by the protozoan Tetrahymena thermophila
    Lawrence A Klobutcher
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    Proc Natl Acad Sci U S A 103:165-70. 2006
    ..We also found that indigestible rinds were generated even from spores in which cross-linking of coat proteins was greatly reduced, implying the existence of a coat structure that is highly resistant to degradative enzymes...
  66. pmc Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor
    Yunfeng Li
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Mol Biol 402:8-16. 2010
    ..Our findings provide the first structural view of the nGR subunits and a molecular framework for understanding the architecture, conservation, and function of nGRs...
  67. ncbi request reprint Mechanisms of killing of Bacillus subtilis spores by hypochlorite and chlorine dioxide
    S B Young
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032 3305, USA
    J Appl Microbiol 95:54-67. 2003
    ..To determine the mechanisms of Bacillus subtilis spore killing by hypochlorite and chlorine dioxide, and its resistance against them...
  68. pmc The Bacillus subtilis dacB gene, encoding penicillin-binding protein 5*, is part of a three-gene operon required for proper spore cortex synthesis and spore core dehydration
    D L Popham
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305, USA
    J Bacteriol 177:4721-9. 1995
    ..The idea that loosely cross-linked, flexible cortex peptidoglycan has a mechanical activity involved in achieving spore core dehydration is not consistent with normal core dehydration in spores lacking only dacB...
  69. pmc New small, acid-soluble proteins unique to spores of Bacillus subtilis: identification of the coding genes and regulation and function of two of these genes
    I Bagyan
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 180:6704-12. 1998
    ..A mutation eliminating SspG had no effect on sporulation or spore properties, while loss of SspJ caused a slight decrease in the rate of spore outgrowth in an otherwise wild-type background...
  70. ncbi request reprint Analysis of the loss in heat and acid resistance during germination of spores of Bacillus species
    Stephanie Luu
    Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 196:1733-40. 2014
    ..Presumably, this altered membrane cannot retain DPA during heat or acid treatments innocuous for dormant spores, resulting in DPA-less spores that are rapidly killed. ..
  71. pmc Germination protein levels and rates of germination of spores of Bacillus subtilis with overexpressed or deleted genes encoding germination proteins
    Kerry Ann V Stewart
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:3156-64. 2012
    ..However, levels of GerB's C subunit, also almost certainly a lipoprotein, and GerK's A subunit were normal in gerF spores. These results with gerF spores were consistent with effects of loss of GerF on spore germination by different GRs...
  72. pmc Levels of germination proteins in dormant and superdormant spores of Bacillus subtilis
    Sonali Ghosh
    Department of Molecular, Microbial, and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut, USA
    J Bacteriol 194:2221-7. 2012
    ....
  73. pmc How moist heat kills spores of Bacillus subtilis
    William H Coleman
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 189:8458-66. 2007
    ....
  74. pmc The products of the spoVA operon are involved in dipicolinic acid uptake into developing spores of Bacillus subtilis
    Federico Tovar-Rojo
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 184:584-7. 2002
    ..These data indicate that one or more products of the spoVA operon are involved in DPA transport into the developing forespore during sporulation...
  75. pmc Effect of a small, acid-soluble spore protein from Clostridium perfringens on the resistance properties of Bacillus subtilis spores
    Juan Francisco Leyva-Illades
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 189:7927-31. 2007
    ..These results further indicate the interchangeability of alpha/beta-type SASP in DNA protection in spores...
  76. pmc Protozoal digestion of coat-defective Bacillus subtilis spores produces "rinds" composed of insoluble coat protein
    Alicia Monroe Carroll
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    Appl Environ Microbiol 74:5875-81. 2008
    ....
  77. pmc Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination
    Guiwen Wang
    Department of Physics, East Carolina University, Greenville, North Carolina 27858 4353, USA
    J Bacteriol 193:2301-11. 2011
    ..iii) Spores release significant CaDPA before commitment. (iv) CaDPA release during T(lag) and ΔT(release) may signal subsequent germination events...
  78. ncbi request reprint Characterization of yhcN, a new forespore-specific gene of Bacillus subtilis
    I Bagyan
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Gene 212:179-88. 1998
    ..A yhcN mutant sporulates normally and yhcN spores have identical resistance properties to wild-type spores. However, the outgrowth of yhcN spores is slower than that of wild-type spores...
  79. ncbi request reprint Analysis of the regulation and function of five genes encoding small, acid-soluble spore proteins of Bacillus subtilis
    A Cabrera-Hernandez
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Gene 248:169-81. 2000
    ..Loss of sspI also had no effect on sporulation, spore properties or spore germination, but DeltasspI spores had a significant defect in spore outgrowth...
  80. pmc Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores
    J L Sanchez-Salas
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06030 3305
    J Bacteriol 175:2568-77. 1993
    ..During spore germination, the GPR from either species expressed in B. subtilis was further processed by removal of one additional amino-terminal amino acid (leucine), generating the mature protease which acts during spore germination...
  81. ncbi request reprint Regulation of four genes encoding small, acid-soluble spore proteins in Bacillus subtilis
    A Cabrera-Hernandez
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    Gene 232:1-10. 1999
    ..Mutations disrupting the sspH, sspL, sspN-tlp, or tlp loci cause a loss of the appropriate SASP from spores, but have no discernible effect on sporulation, spore properties, or spore germination...
  82. pmc Cloning, nucleotide sequence, and regulation of the Bacillus subtilis pbpE operon, which codes for penicillin-binding protein 4* and an apparent amino acid racemase
    D L Popham
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06032
    J Bacteriol 175:2917-25. 1993
    ..This induction depended on the activity of the spo0A product in relieving repression by the abrB repressor. A single transcription start site which was apparently dependent on E sigma A was detected upstream of pbpE...
  83. ncbi request reprint Analysis of factors that influence the sensitivity of spores of Bacillus subtilis to DNA damaging chemicals
    D E Cortezzo
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 98:606-17. 2005
    ..To elucidate factors influencing the sensitivity of Bacillus subtilis spores to DNA damaging chemicals...
  84. pmc Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate
    M Paidhungat
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 183:4886-93. 2001
    ..Our findings thus define a mechanism for Ca(2+)-DPA-induced germination and also provide the first definitive evidence for a signaling pathway that activates cortex hydrolysis in response to nutrients...
  85. pmc Isolation and characterization of superdormant spores of Bacillus species
    Sonali Ghosh
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut 06030 3305, USA
    J Bacteriol 191:1787-97. 2009
    ..A second important factor may be heat activation of spore populations, since yields of superdormant spores from non-heat-activated spore populations were higher than those from optimally activated spores...
  86. pmc Quantitative analysis of spatial-temporal correlations during germination of spores of Bacillus Species
    Jinqiao Zhang
    Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Bacteriol 193:3765-72. 2011
    ..However, two molecules known to be germinants that are released during germination, l-alanine and the 1:1 chelate of Ca²⁺ and dipicolinic acid, did not mediate spore-to-spore communication during germination...
  87. pmc Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis
    Irina Bagyan
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 184:1219-24. 2002
    ....
  88. pmc Synergism between different germinant receptors in the germination of Bacillus subtilis spores
    Xuan Yi
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 193:4664-71. 2011
    ..Direct cooperative action between GRs may further add to the synergism seen in germination triggered by multiple GRs. Further experiments and modeling are required to determine the relative contributions of these different mechanisms...
  89. pmc Mechanisms of induction of germination of Bacillus subtilis spores by high pressure
    Madan Paidhungat
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    Appl Environ Microbiol 68:3172-5. 2002
    ....
  90. ncbi request reprint Analysis of the properties of spores of Bacillus subtilis prepared at different temperatures
    E Melly
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 92:1105-15. 2002
    ..To determine the effect of sporulation temperature on Bacillus subtilis spore resistance and spore composition...
  91. ncbi request reprint Treatment with oxidizing agents damages the inner membrane of spores of Bacillus subtilis and sensitizes spores to subsequent stress
    D E Cortezzo
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 97:838-52. 2004
    ..To determine if treatment of Bacillus subtilis spores with a variety of oxidizing agents causes damage to the spore's inner membrane...
  92. pmc Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties
    B Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 182:6906-12. 2000
    ..These results indicate that alpha/beta-type SASP may well have global effects on gene expression during sporulation and spore outgrowth...
  93. ncbi request reprint Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanol
    B Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030, USA
    J Appl Microbiol 92:362-75. 2002
    ..To determine the mechanisms of killing of Bacillus subtilis spores by ethanol or strong acid or alkali...
  94. ncbi request reprint N-terminal amino acid residues mediate protein-protein interactions between DNA-bound alpha /beta -type small, acid-soluble spore proteins from Bacillus species
    C S Hayes
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    J Biol Chem 276:2267-75. 2001
    ..These results are interpreted in the context of a model for DNA-dependent alpha/beta-type SASP protein-protein interaction involving the N-terminal regions of these proteins...
  95. ncbi request reprint Studies on the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide
    E Melly
    Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA
    J Appl Microbiol 93:316-25. 2002
    ..To determine the mechanism of killing of Bacillus subtilis spores by hydrogen peroxide...
  96. ncbi request reprint Resistance of spores of Bacillus species to ultraviolet light
    P Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    Environ Mol Mutagen 38:97-104. 2001
    ..SP-specific repair is also unique to spores and is carried out by a light-independent SP-lyase, an iron-sulfur protein that utilizes S-adenosylmethionine to catalyze SP monomerization without DNA backbone cleavage...
  97. pmc Properties of spores of Bacillus subtilis blocked at an intermediate stage in spore germination
    B Setlow
    Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA
    J Bacteriol 183:4894-9. 2001
    ..We propose that the germinated spores in which the cortex has not been degraded represent an intermediate stage in spore germination, which we term stage I...
  98. ncbi request reprint Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Clostridium perfringens spores
    R M Cabrera-Martinez
    Department of Biochemistry, University of Connecticut Health Center, Farmington 06032
    FEMS Microbiol Lett 61:127-31. 1991
    ..However, the C. perfringens SASP exhibit significant sequence divergence from Bacillus and C. bifermentans SASP. These genes may be useful in taxonomic studies of Clostridium species...
  99. pmc Extremely variable conservation of γ-type small, acid-soluble proteins from spores of some species in the bacterial order Bacillales
    Jay Vyas
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Bacteriol 193:1884-92. 2011
    ..acidocaldarius spores, although several of the DNA-binding α/β-type SASP were present. These findings have elucidated the phylogenetic origin of the sspE gene, and this may help in determining the precise function of γ-type SASP...
  100. doi request reprint The preparation, germination properties and stability of superdormant spores of Bacillus cereus
    S Ghosh
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT 06030 3305, USA
    J Appl Microbiol 108:582-90. 2010
    ..To determine yields, germination and stability of superdormant Bacillus cereus spores...
  101. ncbi request reprint Effects of modification of membrane lipid composition on Bacillus subtilis sporulation and spore properties
    K K Griffiths
    Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, CT, USA
    J Appl Microbiol 106:2064-78. 2009
    ..To determine effects of inner membrane lipid composition on Bacillus subtilis sporulation and spore properties...