ERIC SELKER

Summary

Affiliation: University of Oregon
Country: USA

Publications

  1. pmc DNA methylation and normal chromosome behavior in Neurospora depend on five components of a histone methyltransferase complex, DCDC
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS Genet 6:e1001196. 2010
  2. ncbi request reprint Epigenetic phenomena in filamentous fungi: useful paradigms or repeat-induced confusion?
    E U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Trends Genet 13:296-301. 1997
  3. ncbi request reprint The methylated component of the Neurospora crassa genome
    Eric U Selker
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Nature 422:893-7. 2003
  4. pmc Induction and maintenance of nonsymmetrical DNA methylation in Neurospora
    Eric U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403 1229, USA
    Proc Natl Acad Sci U S A 99:16485-90. 2002
  5. ncbi request reprint Repeat-induced gene silencing in fungi
    Eric U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Adv Genet 46:439-50. 2002
  6. pmc Relics of repeat-induced point mutation direct heterochromatin formation in Neurospora crassa
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, 97403 1229, USA
    Genome Res 19:427-37. 2009
  7. ncbi request reprint Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa
    Hisashi Tamaru
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Nat Genet 34:75-9. 2003
  8. ncbi request reprint DNA methylation is independent of RNA interference in Neurospora
    Michael Freitag
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Science 304:1939. 2004
  9. pmc Synthesis of signals for de novo DNA methylation in Neurospora crassa
    Hisashi Tamaru
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 23:2379-94. 2003
  10. pmc Direct interaction between DNA methyltransferase DIM-2 and HP1 is required for DNA methylation in Neurospora crassa
    Shinji Honda
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Mol Cell Biol 28:6044-55. 2008

Research Grants

  1. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1992
  2. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1993
  3. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2000
  4. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2001
  5. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2001
  6. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2002
  7. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2002
  8. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2007
  9. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1990
  10. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1999

Collaborators

Detail Information

Publications40

  1. pmc DNA methylation and normal chromosome behavior in Neurospora depend on five components of a histone methyltransferase complex, DCDC
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS Genet 6:e1001196. 2010
    ..DIM-7 is required for DIM-5 localization and mediates interaction of DIM-5 with DDB1/CUL4 through DIM-9. These data support a two-step mechanism for H3K9 methylation in Neurospora...
  2. ncbi request reprint Epigenetic phenomena in filamentous fungi: useful paradigms or repeat-induced confusion?
    E U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Trends Genet 13:296-301. 1997
    ..Together, these processes should limit the proliferation of transposable elements and serve to preserve the overall structure of the genome...
  3. ncbi request reprint The methylated component of the Neurospora crassa genome
    Eric U Selker
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Nature 422:893-7. 2003
    ..DNA sequence analysis shows that the methylated component of the genome consists almost exclusively of relics of transposons that were subject to repeat-induced point mutation--a genome defence system that mutates duplicated sequences...
  4. pmc Induction and maintenance of nonsymmetrical DNA methylation in Neurospora
    Eric U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403 1229, USA
    Proc Natl Acad Sci U S A 99:16485-90. 2002
    ..DNA methylation indirectly recruits histone deacetylases, providing the framework of a self-reinforcing system that could result in propagation of DNA methylation and the associated silenced chromatin state...
  5. ncbi request reprint Repeat-induced gene silencing in fungi
    Eric U Selker
    Institute of Molecular Biology, University of Oregon, Eugene 97403, USA
    Adv Genet 46:439-50. 2002
  6. pmc Relics of repeat-induced point mutation direct heterochromatin formation in Neurospora crassa
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, 97403 1229, USA
    Genome Res 19:427-37. 2009
    ..These data show that A:T-rich RIP'd DNA efficiently directs methylation of H3K9, which in turn, directs methylation of associated cytosines...
  7. ncbi request reprint Trimethylated lysine 9 of histone H3 is a mark for DNA methylation in Neurospora crassa
    Hisashi Tamaru
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Nat Genet 34:75-9. 2003
    ..crassa. Here we show that trimethylated H3 Lys9, but not dimethylated H3 Lys9, marks chromatin regions for cytosine methylation and that DIM-5 specifically creates this mark...
  8. ncbi request reprint DNA methylation is independent of RNA interference in Neurospora
    Michael Freitag
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Science 304:1939. 2004
  9. pmc Synthesis of signals for de novo DNA methylation in Neurospora crassa
    Hisashi Tamaru
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Mol Cell Biol 23:2379-94. 2003
    ....
  10. pmc Direct interaction between DNA methyltransferase DIM-2 and HP1 is required for DNA methylation in Neurospora crassa
    Shinji Honda
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Mol Cell Biol 28:6044-55. 2008
    ....
  11. pmc Robert L. Metzenberg, June 11, 1930-July 15, 2007: geneticist extraordinaire and "model human"
    Eric U Selker
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 178:611-9. 2008
  12. ncbi request reprint HP1 is essential for DNA methylation in neurospora
    Michael Freitag
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 USA
    Mol Cell 13:427-34. 2004
    ..Unlike mutants that lack DNA methyltransferase, mutants with defects in the HP1 gene hpo exhibit severe growth defects, suggesting that HP1 is required for processes besides DNA methylation...
  13. ncbi request reprint GFP as a tool to analyze the organization, dynamics and function of nuclei and microtubules in Neurospora crassa
    Michael Freitag
    Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Fungal Genet Biol 41:897-910. 2004
    ..H1-GFP was translated in the vicinity of hH1+-sgfp+ nuclei in the common cytoplasm of giant Banana ascospores, but it diffused into all nuclei, another illustration of the utility of GFP fusion proteins...
  14. ncbi request reprint Controlling DNA methylation: many roads to one modification
    Michael Freitag
    Institute of Molecular Biology and Department of Biology, University of Oregon, Eugene, OR 97403, USA
    Curr Opin Genet Dev 15:191-9. 2005
    ..Despite recent progress, classic questions remain, including: What are the signals for DNA methylation? Are "de novo" and "maintenance" methylation truly separate processes? How is DNA methylation regulated?..
  15. pmc Methylation of histone H3 lysine 36 is required for normal development in Neurospora crassa
    Keyur K Adhvaryu
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Eukaryot Cell 4:1455-64. 2005
    ..Taken together, our results suggest that methylation of K36 in Neurospora crassa is essential for normal growth and development...
  16. pmc The DMM complex prevents spreading of DNA methylation from transposons to nearby genes in Neurospora crassa
    Shinji Honda
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, 97403, USA
    Genes Dev 24:443-54. 2010
    ..DMM-1 relies on an associated protein, DMM-2, which bears a DNA-binding motif, for localization and proper function. HP1 is required to recruit the DMM complex to the edges of methylated regions...
  17. pmc Identification of DIM-7, a protein required to target the DIM-5 H3 methyltransferase to chromatin
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA
    Proc Natl Acad Sci U S A 107:8310-5. 2010
    ..In addition, we found that DIM-7 is essential for recruitment of DIM-5 to form heterochromatin...
  18. pmc High-density detection of restriction-site-associated DNA markers for rapid mapping of mutated loci in Neurospora
    Zachary A Lewis
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 177:1163-71. 2007
    ..crassa strains: Mauriceville and Oak Ridge. Furthermore, RAD mapping was used to quickly map a previously unknown gene, defective in methylation-7 (dim-7)...
  19. pmc Protein phosphatase PP1 is required for normal DNA methylation in Neurospora
    Keyur K Adhvaryu
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Genes Dev 22:3391-6. 2008
    ..Similarly, amino acid substitutions of H3S10 abolished methylation of H3K9 and DNA. Thus, H3S10 dephosphorylation by PP1 is required for DNA methylation of some loci...
  20. pmc Tools for fungal proteomics: multifunctional neurospora vectors for gene replacement, protein expression and protein purification
    Shinji Honda
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 182:11-23. 2009
    ..Features of the new vectors allowed for verification of an interaction between HP1 and DIM-2 in vivo by Co-IP assays on proteins expressed either from their native loci or from the his-3 locus...
  21. pmc PARP is involved in replicative aging in Neurospora crassa
    Gregory O Kothe
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, United States
    Fungal Genet Biol 47:297-309. 2010
    ..PARPs may regulate aging by impacting NAD+/NAM availability, thereby influencing Sirtuin activity, or they may function in alternative NAD+-dependent or NAD+-independent aging pathways...
  22. doi request reprint Extensive and varied modifications in histone H2B of wild-type and histone deacetylase 1 mutant Neurospora crassa
    D C Anderson
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA
    Biochemistry 49:5244-57. 2010
    ..Combined with asymmetry-generated diversity in H2B structural states in nucleosome core particles, the extensive modifications identified here can create substantial histone-generated structural diversity in nucleosome core particles...
  23. pmc A cytosine methyltransferase homologue is essential for repeat-induced point mutation in Neurospora crassa
    Michael Freitag
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Proc Natl Acad Sci U S A 99:8802-7. 2002
    ..We isolated homologues of rid from Neurospora tetrasperma and Neurospora intermedia to identify conserved regions. Homologues possess all motifs characteristic of eukaryotic DMTs and have large distinctive C- and N-terminal domains...
  24. pmc Identification and characterization of the genes encoding the core histones and histone variants of Neurospora crassa
    Shan M Hays
    Department of Biology and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 1229, USA
    Genetics 160:961-73. 2002
    ..This may be a reflection of the evolution of the RIP (repeat-induced point mutation) and MIP (methylation induced premeiotically) processes that detect sizable duplications and silence associated genes...
  25. pmc Rapid SNP discovery and genetic mapping using sequenced RAD markers
    Nathan A Baird
    Institute of Molecular Biology, University of Oregon, Eugene, Oregon, United States of America
    PLoS ONE 3:e3376. 2008
    ..Sequencing of RAD markers is an integrated platform for SNP discovery and genotyping. This approach should be widely applicable to genetic mapping in a variety of organisms...
  26. pmc Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase
    Xing Zhang
    Department of Biochemistry, School of Medicine, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA
    Cell 111:117-27. 2002
    ..The structure suggests a mechanism for the methylation reaction and provides the structural basis for functional characterization of the HKMT family and the SET domain...
  27. ncbi request reprint Inhibition of DNA methylation and reactivation of silenced genes by zebularine
    Jonathan C Cheng
    University of Southern California Norris Comprehensive Cancer Center and Hospital, Department of Biochemistry and Molecular Biology, USC Keck School of Medicine, Los Angeles 90089, USA
    J Natl Cancer Inst 95:399-409. 2003
    ..We characterized a new demethylating agent, zebularine [1-(beta-D-ribofuranosyl)-1,2-dihydropyrimidin-2-one], which is a chemically stable cytidine analog...
  28. pmc Histone H1 Is required for proper regulation of pyruvate decarboxylase gene expression in Neurospora crassa
    H Diego Folco
    Instituto de Investigacion Medica Mercedes y Martin Ferreyra, Departamento de Quimica Biologica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, 5016 Cordoba, Argentina
    Eukaryot Cell 2:341-50. 2003
    ..Thus, Neurospora histone H1 is required for the proper regulation of cfp, a gene with a key role in the respiratory-fermentative pathway...
  29. ncbi request reprint The genome sequence of the filamentous fungus Neurospora crassa
    James E Galagan
    Whitehead Institute Center for Genome Research, 320 Charles Street, Cambridge, Massachusetts 02141, USA
    Nature 422:859-68. 2003
    ..Genome analysis suggests that RIP has had a profound impact on genome evolution, greatly slowing the creation of new genes through genomic duplication and resulting in a genome with an unusually low proportion of closely related genes...
  30. ncbi request reprint Molecular biology. A self-help guide for a trim genome
    Eric U Selker
    Science 300:1517-8. 2003
  31. pmc Structural basis for the product specificity of histone lysine methyltransferases
    Xing Zhang
    Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA
    Mol Cell 12:177-85. 2003
    ....
  32. pmc In vitro and in vivo analyses of a Phe/Tyr switch controlling product specificity of histone lysine methyltransferases
    Robert E Collins
    Biochemistry and Chemistry, Emory University, Atlanta, GA 30392, USA
    J Biol Chem 280:5563-70. 2005
    ..The altered DIM-5 rescued the growth defect characteristic of dim-5 N. crassa but did not fully rescue the gross DNA hypomethylation of dim-5 strains...
  33. ncbi request reprint RIP: the evolutionary cost of genome defense
    James E Galagan
    Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 04124, USA
    Trends Genet 20:417-23. 2004
    ..Most if not all paralogs in N. crassa duplicated and diverged before the emergence of RIP. Thus, RIP illustrates the extraordinary extent to which genomes will go to defend themselves against mobile genetic elements...
  34. pmc Recurrent locus-specific mutation resulting from a cryptic ectopic insertion in Neurospora
    David D Perkins
    Department of Biological Sciences, Stanford University, Stanford, California 94305 5020, USA
    Genetics 175:527-44. 2007
    ..These findings support a model in which an ectopic insertion that created a mutation at the target site acts as a locus-specific mutator via RIP...
  35. pmc The Neurospora crassa White Collar-1 dependent blue light response requires acetylation of histone H3 lysine 14 by NGF-1
    Benedetto Grimaldi
    Dipartimento di Genetica e Biologia Molecolare, Universita di Roma La Sapienza, 00185 Rome, Italy
    Mol Biol Cell 17:4576-83. 2006
    ..Together, our findings reveal a direct link between histone modification and light signaling in Neurospora and contribute to the developing understanding of the molecular mechanisms operating in light-inducible gene activation...
  36. pmc Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism
    Katherine A Borkovich
    Department of Plant Pathology, University of California, Riverside, California 92521, USA
    Microbiol Mol Biol Rev 68:1-108. 2004
    ..The group of unshared genes includes potential new targets for antifungals as well as loci implicated in human and plant physiology and disease...
  37. pmc A cytosine methyltransferase homologue is essential for sexual development in Aspergillus nidulans
    Dong W Lee
    Department of Biology, College of Science, Texas A and M University, College Station, Texas, United States of America
    PLoS ONE 3:e2531. 2008
    ..We discovered a close homologue in Aspergillus nidulans, a fungus thought to have no methylation and no genome defense system comparable to RIP or MIP...
  38. pmc A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes
    Richard J Roberts
    New England Biolabs, Beverly, MA 01915, USA
    Nucleic Acids Res 31:1805-12. 2003
    ..It provides explicit categories for the many different Type II enzymes now identified and provides a system for naming the putative genes found by sequence analysis of microbial genomes...
  39. ncbi request reprint Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae
    James E Galagan
    The Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, Massachusetts 02142, USA
    Nature 438:1105-15. 2005
    ..These results enhance our understanding of these widely studied fungi as well as provide new insight into eukaryotic genome evolution and gene regulation...
  40. pmc The 2005 Thomas Hunt Morgan Medal. Robert L. Metzenberg
    Eric U Selker
    Genetics 169:503-5. 2005

Research Grants21

  1. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1992
    ..Mutants defective in other genes necessary for methylation will be isolated using two approaches - one using a selection successful in a preliminary experiment, and the other using "brute force" to obtain all possible mutants...
  2. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1993
    ..Mutants defective in other genes necessary for methylation will be isolated using two approaches - one using a selection successful in a preliminary experiment, and the other using "brute force" to obtain all possible mutants...
  3. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2000
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  4. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2001
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  5. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2001
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  6. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2002
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  7. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2002
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  8. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2007
    ..To test the possible role of chromatin remodeling in DNA methylation; 7. To identify additional components of the DNA methvlation machinerv. ..
  9. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1990
  10. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1999
    ..Methylation mutants and methods that we have developed to study replication in Neurospora will be used to investigate whether either DNA methylation or histone hypoacetylation cause late replication of DNA. ..
  11. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 1991
    ..Mutants defective in other genes necessary for methylation will be isolated using two approaches - one using a selection successful in a preliminary experiment, and the other using "brute force" to obtain all possible mutants...
  12. GENETIC ASPECTS OF DNA METHYLATION
    ERIC SELKER; Fiscal Year: 2008
    ..Finally, we will follow up our observations suggesting that histone demethylases influence DNA methylation. ..