Research Topics
Genomes and Genes | William G ScottSummaryAffiliation: University of California Country: USA Publications
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Publications
Visualizing the structure and mechanism of a small nucleolytic ribozymeWilliam G Scott
Department of Chemistry and Biochemistry, Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
Methods 28:302-6. 2002..The application of monochromatic time-resolved X-ray crystallographic freeze-trapping experiments to an RNA enzyme, the hammerhead ribozyme, is outlined here...
The structure of a rigorously conserved RNA element within the SARS virus genomeMichael P Robertson
The Center for the Molecular Biology of RNA, University of California, Santa Cruz, USA
PLoS Biol 3:e5. 2005....- Morphing the minimal and full-length hammerhead ribozymes: implications for the cleavage mechanismWilliam G Scott
The Center for the Molecular Biology of RNA, 228 Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
Biol Chem 388:727-35. 2007.... 
Structure and function of regulatory RNA elements: ribozymes that regulate gene expressionWilliam G Scott
Center for the Molecular Biology of RNA, Sinsheimer Laboratory, University of California at Santa Cruz, Santa Cruz, CA, USA
Biochim Biophys Acta 1789:634-41. 2009..With the discovery of a mammalian riboswitch ribozyme, the question of how an embedded hammerhead ribozyme's switching mechanism works becomes a compelling question. Recent structural results suggest several possibilities...- Ribozyme catalysis via orbital steeringW G Scott
Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
J Mol Biol 311:989-99. 2001..Orbital steering therefore appears to be a general phenomenon that may help to explain catalysis in both ribozymes and protein enzymes in a unified manner... - RibozymesWilliam G Scott
Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
Curr Opin Struct Biol 17:280-6. 2007..Although not all ribozymes are metalloenzymes, acid-base catalysis appears to be a universal property shared by all ribozymes as well as many of their protein cousins... - The structural basis of ribozyme-catalyzed RNA assemblyMichael P Robertson
Center for the Molecular Biology of RNA and Department of Chemistry and Biochemistry, Robert L Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Science 315:1549-53. 2007..The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly... - Tertiary contacts distant from the active site prime a ribozyme for catalysisMonika Martick
Department of Molecular, Cellular and Developmental Biology, Robert L. Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Cell 126:309-20. 2006..These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner... - A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reactionJames B Murray
Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
J Mol Biol 315:121-30. 2002..We further hypothesize that deprotonation of the cleavage-site 2'-oxygen drives this conformational change... - Solvent structure and hammerhead ribozyme catalysisMonika Martick
Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
Chem Biol 15:332-42. 2008..Phosphate-bridging metal interactions and other mechanistic hypotheses are also tested with this approach... - A helical twist-induced conformational switch activates cleavage in the hammerhead ribozymeChristine M Dunham
Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
J Mol Biol 332:327-36. 2003..Taken together, these results permit us to suggest that switching between ligase and nuclease activity is determined by the helical orientation of Stem I relative to Stem II... - A general method for phasing novel complex RNA crystal structures without heavy-atom derivativesMichael P Robertson
Department of Chemistry and Biochemistry and The Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
Acta Crystallogr D Biol Crystallogr . 2008..This approach has also proven successful with other ribozymes, structured RNAs and RNA-protein complexes... - Solving novel RNA structures using only secondary structural fragmentsMichael P Robertson
Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
Methods 52:168-72. 2010.... - The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations aloneJ B Murray
Department of Chemistry and Biochemistry, University of California, Santa Cruz, USA
Chem Biol 5:587-95. 1998..The catalytic activity of RNA enzymes is thought to require divalent metal ions, which are believed to facilitate RNA folding and to play a direct chemical role in the reaction... - RNA catalysisW G Scott
Department of Chemistry and Biochemistry The Center for Molecular Biology of RNA Sinsheimer Laboratories University of California Santa Cruz Santa Cruz CA 95064 USA
Curr Opin Struct Biol 8:720-6. 1998.... - A distal arginine in oxygen-sensing heme-PAS domains is essential to ligand binding, signal transduction, and structureChristine M Dunham
Chemistry and Biochemistry Department, University of California, 236 Sinsheimer Labs, Santa Cruz, California 95064, USA
Biochemistry 42:7701-8. 2003..These findings support a regulatory model in which the heme-PAS domain operates as an ensemble that couples to the kinase rather than a mechanism driven by a single central switch... - A discontinuous hammerhead ribozyme embedded in a mammalian messenger RNAMonika Martick
Center for Molecular Biology of RNA, University of California, Santa Cruz, California 95064, USA
Nature 454:899-902. 2008.... - Crystal structure of a translation termination complex formed with release factor RF2Andrei Korostelev
Center for Molecular Biology of RNA and Departments of Molecular, Cell and Developmental Biology and Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
Proc Natl Acad Sci U S A 105:19684-9. 2008..We show that when the H-bonding capability of the main-chain N-H of the conserved glutamine is eliminated by substitution with proline, peptidyl-tRNA esterase activity is abolished, consistent with its proposed role in catalysis... - Molecular features of the copper binding sites in the octarepeat domain of the prion proteinColin S Burns
Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
Biochemistry 41:3991-4001. 2002.... - Novel ring structure in the gp41 trimer of human immunodeficiency virus type 1 that modulates sensitivity and resistance to broadly neutralizing antibodiesSara M O'Rourke
Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, USA
J Virol 83:7728-38. 2009.... - Challenges and surprises that arise with nucleic acids during model building and refinementWilliam G Scott
Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
Acta Crystallogr D Biol Crystallogr 68:441-5. 2012.... - RNA structure, metal ions, and catalysisW G Scott
Department of Chemistry and Biochemistry, Sinsheimer Laboratories, The Center for the Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, 95064, USA edu
Curr Opin Chem Biol 3:705-9. 1999..From a mechanistic point of view, the NMR and crystal structures of a small Pb(2+)-dependent ribozyme have been particularly revealing... - Capturing hammerhead ribozyme structures in action by modulating general base catalysisYoung In Chi
Department of Molecular and Cellular Biochemistry, Center for Structural Biology, University of Kentucky, Lexington, Kentucky, United States of America
PLoS Biol 6:e234. 2008..The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures... - Structure of Escherichia coli RNase E catalytic domain and implications for RNA turnoverAnastasia J Callaghan
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
Nature 437:1187-91. 2005..The structure explains how the recognition of the 5' terminus of the substrate may trigger catalysis and also sheds light on the question of how RNase E might selectively process, rather than destroy, specific RNA precursors... - Role of Mg2+ in hammerhead ribozyme catalysis from molecular simulationTai Sung Lee
Consortium for Bioinformatics and Computational Biology, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
J Am Chem Soc 130:3053-64. 2008.... - Catalysis, evolution and lifeAbraham Szoke
ICM Molecular Biophysics, Uppsala University, Sweden
FEBS Lett 553:18-20. 2003.... - The crystal structure of the Escherichia coli RNase E apoprotein and a mechanism for RNA degradationDaniel J Koslover
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
Structure 16:1238-44. 2008....
Research Grants
- STRUCTURE AND CATALYSIS OF AN RNA ENZYMEWilliam Scott; Fiscal Year: 2000....
- STRUCTURE AND CATALYSIS OF AN RNA ENZYMEWilliam Scott; Fiscal Year: 2007..abstract_text> ..