William G Scott

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Visualizing the structure and mechanism of a small nucleolytic ribozyme
    William G Scott
    Department of Chemistry and Biochemistry, Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
    Methods 28:302-6. 2002
  2. pmc The structure of a rigorously conserved RNA element within the SARS virus genome
    Michael P Robertson
    The Center for the Molecular Biology of RNA, University of California, Santa Cruz, USA
    PLoS Biol 3:e5. 2005
  3. ncbi request reprint Morphing the minimal and full-length hammerhead ribozymes: implications for the cleavage mechanism
    William G Scott
    The Center for the Molecular Biology of RNA, 228 Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Biol Chem 388:727-35. 2007
  4. doi request reprint Structure and function of regulatory RNA elements: ribozymes that regulate gene expression
    William G Scott
    Center for the Molecular Biology of RNA, Sinsheimer Laboratory, University of California at Santa Cruz, Santa Cruz, CA, USA
    Biochim Biophys Acta 1789:634-41. 2009
  5. ncbi request reprint Ribozyme catalysis via orbital steering
    W G Scott
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 311:989-99. 2001
  6. ncbi request reprint Ribozymes
    William G Scott
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Curr Opin Struct Biol 17:280-6. 2007
  7. ncbi request reprint The structural basis of ribozyme-catalyzed RNA assembly
    Michael P Robertson
    Center for the Molecular Biology of RNA and Department of Chemistry and Biochemistry, Robert L Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Science 315:1549-53. 2007
  8. ncbi request reprint Tertiary contacts distant from the active site prime a ribozyme for catalysis
    Monika Martick
    Department of Molecular, Cellular and Developmental Biology, Robert L Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Cell 126:309-20. 2006
  9. ncbi request reprint A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction
    James B Murray
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 315:121-30. 2002
  10. pmc Solvent structure and hammerhead ribozyme catalysis
    Monika Martick
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Chem Biol 15:332-42. 2008

Research Grants

  1. STRUCTURE AND CATALYSIS OF AN RNA ENZYME
    William Scott; Fiscal Year: 2000
  2. STRUCTURE AND CATALYSIS OF AN RNA ENZYME
    William Scott; Fiscal Year: 2007

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Visualizing the structure and mechanism of a small nucleolytic ribozyme
    William G Scott
    Department of Chemistry and Biochemistry, Center for Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
    Methods 28:302-6. 2002
    ..The application of monochromatic time-resolved X-ray crystallographic freeze-trapping experiments to an RNA enzyme, the hammerhead ribozyme, is outlined here...
  2. pmc The structure of a rigorously conserved RNA element within the SARS virus genome
    Michael P Robertson
    The Center for the Molecular Biology of RNA, University of California, Santa Cruz, USA
    PLoS Biol 3:e5. 2005
    ....
  3. ncbi request reprint Morphing the minimal and full-length hammerhead ribozymes: implications for the cleavage mechanism
    William G Scott
    The Center for the Molecular Biology of RNA, 228 Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Biol Chem 388:727-35. 2007
    ....
  4. doi request reprint Structure and function of regulatory RNA elements: ribozymes that regulate gene expression
    William G Scott
    Center for the Molecular Biology of RNA, Sinsheimer Laboratory, University of California at Santa Cruz, Santa Cruz, CA, USA
    Biochim Biophys Acta 1789:634-41. 2009
    ..With the discovery of a mammalian riboswitch ribozyme, the question of how an embedded hammerhead ribozyme's switching mechanism works becomes a compelling question. Recent structural results suggest several possibilities...
  5. ncbi request reprint Ribozyme catalysis via orbital steering
    W G Scott
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 311:989-99. 2001
    ..Orbital steering therefore appears to be a general phenomenon that may help to explain catalysis in both ribozymes and protein enzymes in a unified manner...
  6. ncbi request reprint Ribozymes
    William G Scott
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Curr Opin Struct Biol 17:280-6. 2007
    ..Although not all ribozymes are metalloenzymes, acid-base catalysis appears to be a universal property shared by all ribozymes as well as many of their protein cousins...
  7. ncbi request reprint The structural basis of ribozyme-catalyzed RNA assembly
    Michael P Robertson
    Center for the Molecular Biology of RNA and Department of Chemistry and Biochemistry, Robert L Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Science 315:1549-53. 2007
    ..The structure of the active site permits us to suggest how transition-state stabilization and a general base may catalyze the ligation reaction required for prebiotic RNA assembly...
  8. ncbi request reprint Tertiary contacts distant from the active site prime a ribozyme for catalysis
    Monika Martick
    Department of Molecular, Cellular and Developmental Biology, Robert L Sinsheimer Laboratories, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Cell 126:309-20. 2006
    ..These interactions permit us to explain the previously irreconcilable sets of experimental results in a unified, consistent, and unambiguous manner...
  9. ncbi request reprint A pH-dependent conformational change, rather than the chemical step, appears to be rate-limiting in the hammerhead ribozyme cleavage reaction
    James B Murray
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 315:121-30. 2002
    ..We further hypothesize that deprotonation of the cleavage-site 2'-oxygen drives this conformational change...
  10. pmc Solvent structure and hammerhead ribozyme catalysis
    Monika Martick
    Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, USA
    Chem Biol 15:332-42. 2008
    ..Phosphate-bridging metal interactions and other mechanistic hypotheses are also tested with this approach...
  11. ncbi request reprint A helical twist-induced conformational switch activates cleavage in the hammerhead ribozyme
    Christine M Dunham
    Department of Chemistry and Biochemistry, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    J Mol Biol 332:327-36. 2003
    ..Taken together, these results permit us to suggest that switching between ligase and nuclease activity is determined by the helical orientation of Stem I relative to Stem II...
  12. pmc A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives
    Michael P Robertson
    Department of Chemistry and Biochemistry and The Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Acta Crystallogr D Biol Crystallogr . 2008
    ..This approach has also proven successful with other ribozymes, structured RNAs and RNA-protein complexes...
  13. pmc Solving novel RNA structures using only secondary structural fragments
    Michael P Robertson
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, University of California, Santa Cruz, CA 95064, USA
    Methods 52:168-72. 2010
    ....
  14. ncbi request reprint The hammerhead, hairpin and VS ribozymes are catalytically proficient in monovalent cations alone
    J B Murray
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, USA
    Chem Biol 5:587-95. 1998
    ..The catalytic activity of RNA enzymes is thought to require divalent metal ions, which are believed to facilitate RNA folding and to play a direct chemical role in the reaction...
  15. ncbi request reprint RNA catalysis
    W G Scott
    Department of Chemistry and Biochemistry The Center for Molecular Biology of RNA Sinsheimer Laboratories University of California Santa Cruz Santa Cruz CA 95064 USA
    Curr Opin Struct Biol 8:720-6. 1998
    ....
  16. ncbi request reprint A distal arginine in oxygen-sensing heme-PAS domains is essential to ligand binding, signal transduction, and structure
    Christine M Dunham
    Chemistry and Biochemistry Department, University of California, 236 Sinsheimer Labs, Santa Cruz, California 95064, USA
    Biochemistry 42:7701-8. 2003
    ..These findings support a regulatory model in which the heme-PAS domain operates as an ensemble that couples to the kinase rather than a mechanism driven by a single central switch...
  17. pmc A discontinuous hammerhead ribozyme embedded in a mammalian messenger RNA
    Monika Martick
    Center for Molecular Biology of RNA, University of California, Santa Cruz, California 95064, USA
    Nature 454:899-902. 2008
    ....
  18. pmc Crystal structure of a translation termination complex formed with release factor RF2
    Andrei Korostelev
    Center for Molecular Biology of RNA and Departments of Molecular, Cell and Developmental Biology and Chemistry and Biochemistry, University of California, Santa Cruz, CA 95064, USA
    Proc Natl Acad Sci U S A 105:19684-9. 2008
    ..We show that when the H-bonding capability of the main-chain N-H of the conserved glutamine is eliminated by substitution with proline, peptidyl-tRNA esterase activity is abolished, consistent with its proposed role in catalysis...
  19. pmc Molecular features of the copper binding sites in the octarepeat domain of the prion protein
    Colin S Burns
    Department of Chemistry and Biochemistry, University of California, Santa Cruz, California 95064, USA
    Biochemistry 41:3991-4001. 2002
    ....
  20. pmc Novel ring structure in the gp41 trimer of human immunodeficiency virus type 1 that modulates sensitivity and resistance to broadly neutralizing antibodies
    Sara M O'Rourke
    Department of Biomolecular Engineering, University of California, Santa Cruz, CA 95064, USA
    J Virol 83:7728-38. 2009
    ....
  21. pmc Challenges and surprises that arise with nucleic acids during model building and refinement
    William G Scott
    Department of Chemistry and Biochemistry and The Center for the Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA 95064, USA
    Acta Crystallogr D Biol Crystallogr 68:441-5. 2012
    ....
  22. ncbi request reprint RNA structure, metal ions, and catalysis
    W G Scott
    Department of Chemistry and Biochemistry, Sinsheimer Laboratories, The Center for the Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, 95064, USA edu
    Curr Opin Chem Biol 3:705-9. 1999
    ..From a mechanistic point of view, the NMR and crystal structures of a small Pb(2+)-dependent ribozyme have been particularly revealing...
  23. pmc Capturing hammerhead ribozyme structures in action by modulating general base catalysis
    Young In Chi
    Department of Molecular and Cellular Biochemistry, Center for Structural Biology, University of Kentucky, Lexington, Kentucky, United States of America
    PLoS Biol 6:e234. 2008
    ..The predissociation enzyme-product complex structure reveals RNA and metal ion interactions potentially relevant to transition-state stabilization that are absent in precatalytic structures...
  24. ncbi request reprint Structure of Escherichia coli RNase E catalytic domain and implications for RNA turnover
    Anastasia J Callaghan
    Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK
    Nature 437:1187-91. 2005
    ..The structure explains how the recognition of the 5' terminus of the substrate may trigger catalysis and also sheds light on the question of how RNase E might selectively process, rather than destroy, specific RNA precursors...
  25. pmc Role of Mg2+ in hammerhead ribozyme catalysis from molecular simulation
    Tai Sung Lee
    Consortium for Bioinformatics and Computational Biology, University of Minnesota, 207 Pleasant Street SE, Minneapolis, Minnesota 55455, USA
    J Am Chem Soc 130:3053-64. 2008
    ....
  26. ncbi request reprint Catalysis, evolution and life
    Abraham Szoke
    ICM Molecular Biophysics, Uppsala University, Sweden
    FEBS Lett 553:18-20. 2003
    ....
  27. pmc The crystal structure of the Escherichia coli RNase E apoprotein and a mechanism for RNA degradation
    Daniel J Koslover
    Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1GA, United Kingdom
    Structure 16:1238-44. 2008
    ....

Research Grants8

  1. STRUCTURE AND CATALYSIS OF AN RNA ENZYME
    William Scott; Fiscal Year: 2000
    ....
  2. STRUCTURE AND CATALYSIS OF AN RNA ENZYME
    William Scott; Fiscal Year: 2007
    ..abstract_text> ..