John D Scott

Summary

Affiliation: University of Washington
Country: USA

Publications

  1. pmc Cell signaling in space and time: where proteins come together and when they're apart
    John D Scott
    Department of Pharmacology, Howard Hughes Medical Institute, Box 357750, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 326:1220-4. 2009
  2. pmc AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency
    Matthew G Gold
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
    EMBO Mol Med 4:15-26. 2012
  3. pmc AKAP220 protein organizes signaling elements that impact cell migration
    Jeremy S Logue
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 286:39269-81. 2011
  4. pmc Anchored protein kinase A recruitment of active Rac GTPase
    Jeremy S Logue
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 286:22113-21. 2011
  5. pmc Shedding light on local kinase activation
    John D Scott
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, 1959 Pacific Ave, NE, Seattle, WA 98195 USA
    BMC Biol 10:61. 2012
  6. pmc Anchored phosphatases modulate glucose homeostasis
    Simon A Hinke
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    EMBO J 31:3991-4004. 2012
  7. pmc AKAP-Lbc mobilizes a cardiac hypertrophy signaling pathway
    Graeme K Carnegie
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Cell 32:169-79. 2008
  8. pmc Role for A kinase-anchoring proteins (AKAPS) in glutamate receptor trafficking and long term synaptic depression
    Eric M Snyder
    Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:16962-8. 2005
  9. pmc Ubiquitination regulates PSD-95 degradation and AMPA receptor surface expression
    Marcie Colledge
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Neuron 40:595-607. 2003
  10. ncbi request reprint AKAP-Lbc nucleates a protein kinase D activation scaffold
    Graeme K Carnegie
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, Portland, OR 97239, USA
    Mol Cell 15:889-99. 2004

Collaborators

Detail Information

Publications70

  1. pmc Cell signaling in space and time: where proteins come together and when they're apart
    John D Scott
    Department of Pharmacology, Howard Hughes Medical Institute, Box 357750, University of Washington School of Medicine, Seattle, WA 98195, USA
    Science 326:1220-4. 2009
    ..This requires that the activators, effectors, enzymes, and substrates that respond to cellular signals come together when they need to...
  2. pmc AKAP2 anchors PKA with aquaporin-0 to support ocular lens transparency
    Matthew G Gold
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA, USA
    EMBO Mol Med 4:15-26. 2012
    ..Thus, anchored PKA modulation of AQP0 is a homeostatic mechanism that must be physically intact to preserve lens transparency...
  3. pmc AKAP220 protein organizes signaling elements that impact cell migration
    Jeremy S Logue
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 286:39269-81. 2011
    ..This reveals an unappreciated role for this anchored kinase/microtubule effector protein network in the propagation of cell motility...
  4. pmc Anchored protein kinase A recruitment of active Rac GTPase
    Jeremy S Logue
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 286:22113-21. 2011
    ..These findings imply that IQGAP2 and AKAP220 act synergistically to sustain PKA-mediated recruitment of effectors such as Rac GTPases that impact the actin cytoskeleton...
  5. pmc Shedding light on local kinase activation
    John D Scott
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, 1959 Pacific Ave, NE, Seattle, WA 98195 USA
    BMC Biol 10:61. 2012
    ..We discuss here how biosensors are being used to decode this language, affording an unprecedented glimpse into spatio-temporal patterns of protein phosphorylation events within the cell...
  6. pmc Anchored phosphatases modulate glucose homeostasis
    Simon A Hinke
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    EMBO J 31:3991-4004. 2012
    ..Thus anchored signalling events that facilitate insulin secretion and glucose homeostasis may be set by AKAP150 associated phosphatase activity...
  7. pmc AKAP-Lbc mobilizes a cardiac hypertrophy signaling pathway
    Graeme K Carnegie
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Mol Cell 32:169-79. 2008
    ..These studies uncover a role for AKAP-Lbc in which increased expression of the anchoring protein selectively amplifies a signaling pathway that drives cardiac myocytes toward a pathophysiological outcome...
  8. pmc Role for A kinase-anchoring proteins (AKAPS) in glutamate receptor trafficking and long term synaptic depression
    Eric M Snyder
    Howard Hughes Medical Institute, The Picower Center for Learning and Memory and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:16962-8. 2005
    ..These data indicate that synaptic anchoring of PKA through association with AKAPs plays an important role in the regulation of AMPA receptor surface expression and synaptic plasticity...
  9. pmc Ubiquitination regulates PSD-95 degradation and AMPA receptor surface expression
    Marcie Colledge
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Neuron 40:595-607. 2003
    ..These data suggest that ubiquitination of PSD-95 through an Mdm2-mediated pathway is critical in regulating AMPA receptor surface expression during synaptic plasticity...
  10. ncbi request reprint AKAP-Lbc nucleates a protein kinase D activation scaffold
    Graeme K Carnegie
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, Portland, OR 97239, USA
    Mol Cell 15:889-99. 2004
    ..This configuration illustrates the utility of kinase anchoring as a mechanism to constrain the action of broad-spectrum enzymes...
  11. pmc Loss of AKAP150 perturbs distinct neuronal processes in mice
    Brian J Tunquist
    Howard Hughes Medical Institute, Oregon Health and Science University, 3181 South West Sam Jackson Park Road, Portland, Oregon 97239, USA
    Proc Natl Acad Sci U S A 105:12557-62. 2008
    ..These studies argue that distinct AKAP150-enzyme complexes regulate context-dependent neuronal signaling events in vivo...
  12. pmc Increased coupled gating of L-type Ca2+ channels during hypertension and Timothy syndrome
    Manuel F Navedo
    Department of Physiology and Biophysics, University of Washington Seattle, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Circ Res 106:748-56. 2010
    ..L-Type (Cav1.2) Ca(2+) channels are critical regulators of muscle and neural function. Although Cav1.2 channel activity varies regionally, little is known about the mechanisms underlying this heterogeneity...
  13. pmc Engineering A-kinase anchoring protein (AKAP)-selective regulatory subunits of protein kinase A (PKA) through structure-based phage selection
    Matthew G Gold
    Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195, USA
    J Biol Chem 288:17111-21. 2013
    ..We propose that this structure-directed evolution strategy might be generally applicable for the investigation of other protein interaction surfaces...
  14. pmc Bioinformatic design of A-kinase anchoring protein-in silico: a potent and selective peptide antagonist of type II protein kinase A anchoring
    Neal M Alto
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland OR 97239, USA
    Proc Natl Acad Sci U S A 100:4445-50. 2003
    ..Thus, computer-based and peptide array screening approaches have generated a reagent that binds PKA with higher affinity than previously described AKAPs...
  15. pmc Dynamic regulation of cAMP synthesis through anchored PKA-adenylyl cyclase V/VI complexes
    Andrea L Bauman
    Howard Hughes Medical Institute, Vollum Institute, L 474, Oregon Health and Science University, 3181 Southwest Sam Jackson Park Road, Portland, Oregon 97239, USA
    Mol Cell 23:925-31. 2006
    ..This protein configuration permits the formation of a negative feedback loop that temporally regulates cAMP production...
  16. ncbi request reprint Regulation of GluR1 by the A-kinase anchoring protein 79 (AKAP79) signaling complex shares properties with long-term depression
    Steven J Tavalin
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health Sciences University, Portland, Oregon 97201, USA
    J Neurosci 22:3044-51. 2002
    ..Thus, the integration of intracellular signals relevant for LTD may be transduced to GluR1 by the AKAP79 signaling complex...
  17. ncbi request reprint AKAP79-mediated targeting of the cyclic AMP-dependent protein kinase to the beta1-adrenergic receptor promotes recycling and functional resensitization of the receptor
    Lidia A Gardner
    Department of Pharmacology, University of Tennessee Health Sciences Center, Memphis, Tennessee 38163, USA
    J Biol Chem 281:33537-53. 2006
    ..Thus, AKAP79 provides PKA to phosphorylate the beta(1)-AR and thereby dictate the recycling and resensitization itineraries of the beta(1)-AR...
  18. pmc Restoration of normal L-type Ca2+ channel function during Timothy syndrome by ablation of an anchoring protein
    Edward P Cheng
    Department of Physiology and Biophysics, University of Washington, 1705 NE Pacific Street, Seattle, WA 98195, USA
    Circ Res 109:255-61. 2011
    ..2 channels causes long QT syndrome 8 (LQT8), a disease also known as Timothy syndrome. However, the mechanisms by which this mutation enhances Ca(V)1.2-LQT8 currents and generates lethal arrhythmias are unclear...
  19. pmc Association of an A-kinase-anchoring protein signaling scaffold with cadherin adhesion molecules in neurons and epithelial cells
    Jessica A Gorski
    Department of Pharmacology, University of Colorado at Denver and Health Sciences Center, Aurora, CO 80045, USA
    Mol Biol Cell 16:3574-90. 2005
    ..This neuronal regulation of AKAP79/150 targeting to cadherins may be important in functional and structural synaptic modifications underlying plasticity...
  20. pmc Interaction with AKAP79 modifies the cellular pharmacology of PKC
    Naoto Hoshi
    Department of Pharmacology, 360 Med Surge II, University of California, Irvine, Irvine, CA 92697, USA
    Mol Cell 37:541-50. 2010
    ..This implies that intracellular binding partners not only couple individual molecular events in a cell signaling process but can also change the pharmacological profile of certain protein kinases...
  21. pmc A WAVE-1 and WRP signaling complex regulates spine density, synaptic plasticity, and memory
    Scott H Soderling
    Howard Hughes Medical Institute, Oregon Health and Science University, Portland, Oregon 97239, USA
    J Neurosci 27:355-65. 2007
    ..This implies that signaling through WAVE-1 complexes is essential for neural plasticity and cognitive behavior...
  22. pmc Architecture and dynamics of an A-kinase anchoring protein 79 (AKAP79) signaling complex
    Matthew G Gold
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, 1959 Pacific Avenue NE, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 108:6426-31. 2011
    ..This leads to activation of the anchored phosphatases. Our architectural model reveals how dimeric AKAP79 concentrates pockets of second messenger responsive enzyme activities at the plasma membrane...
  23. pmc Distinct enzyme combinations in AKAP signalling complexes permit functional diversity
    Naoto Hoshi
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97239, USA
    Nat Cell Biol 7:1066-73. 2005
    ..Utilization of distinct enzyme combinations in this manner provides a means to expand the repertoire of cellular events that the same AKAP modulates...
  24. pmc AKAP-Lbc enhances cyclic AMP control of the ERK1/2 cascade
    F Donelson Smith
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, 1959 Pacific Avenue NE, Seattle, WA 98195, USA
    Nat Cell Biol 12:1242-9. 2010
    ..This offers mechanistic insight into cAMP-responsive control of ERK signalling events...
  25. pmc An entirely specific type I A-kinase anchoring protein that can sequester two molecules of protein kinase A at mitochondria
    Christopher K Means
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington School of Medicine, 1959 Pacific Avenue NE, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 108:E1227-35. 2011
    ..Imaging, proteomic analysis, and subcellular fractionation experiments reveal that SKIP is enriched at the inner mitochondrial membrane where it associates with a prominent PKA substrate, the coiled-coil helix protein ChChd3...
  26. ncbi request reprint Intracellular targeting of protein kinases and phosphatases
    Neal Alto
    Howard Hughes Medical Institute, Vollum Institute, Portland, Oregon 97201 3098, USA
    Diabetes 51:S385-8. 2002
    ....
  27. pmc A-kinase anchoring proteins: from protein complexes to physiology and disease
    Graeme K Carnegie
    Department of Pharmacology, Howard Hughes Medical Institute, University of Washington, School of Medicine, Seattle, Washington 98195, USA
    IUBMB Life 61:394-406. 2009
    ..These anchoring proteins form multi-protein complexes that integrate cAMP signaling with other pathways and signaling events. In this review, we focus on recent advances in the elucidation of AKAP function...
  28. pmc Gravin is a transitory effector of polo-like kinase 1 during cell division
    David A Canton
    Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA
    Mol Cell 48:547-59. 2012
    ..In situ detection of phospho-T766 Gravin in biopsy sections of human glioblastomas suggests that this phosphorylation event might identify malignant neoplasms...
  29. pmc Coordinated signal integration at the M-type potassium channel upon muscarinic stimulation
    Anastasia Kosenko
    Department of Pharmacology, University of California, Irvine, CA 92697, USA
    EMBO J 31:3147-56. 2012
    ..This pathway enhances the effect of concomitant reduction of PIP2, which leads to disruption of the M-channel function. These findings clarify how a common lipid cofactor, such as PIP2, can selectively regulate ion channels...
  30. ncbi request reprint AKAP signaling complexes: getting to the heart of the matter
    George McConnachie
    Howard Hughes Medical Institute, Vollum Institute, L 474, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97239, USA
    Trends Mol Med 12:317-23. 2006
    ..These are only two examples of how AKAPs contribute to specificity in cAMP signaling. This review will explore recent development that illustrates the role of multiprotein complexes in the regulation of cAMP signaling...
  31. ncbi request reprint Contextual utilization of enzymes in discrete AKAP79/150 signalling complexes
    Naoto Hoshi
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, 3181 S W Sam Jackson Park Road, Portland, OR 97239, USA
    Eur J Cell Biol 85:621-2. 2006
    ..Utilization of distinct enzyme combinations in this manner provides a means to expand the repertoire of cellular events that the same AKAP modulates...
  32. pmc Mapping the protein phosphatase-2B anchoring site on AKAP79. Binding and inhibition of phosphatase activity are mediated by residues 315-360
    MARK L DELL'ACQUA
    Howard Hughes Medical Institute, Oregon Health Sciences University, Portland 97201, USA
    J Biol Chem 277:48796-802. 2002
    ..We propose that the structural features of this AKAP79-PP2B-binding domain may share similarities with other proteins that serve to coordinate PP2B localization and activity...
  33. pmc Organizing signal transduction through A-kinase anchoring proteins (AKAPs)
    Jeremy S Logue
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    FEBS J 277:4370-5. 2010
    ..Selected A-kinase anchoring protein signaling complexes are highlighted in this minireview...
  34. ncbi request reprint A-kinase anchoring proteins and neuronal signaling mechanisms
    Graeme K Carnegie
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, Portland, Oregon 97201, USA
    Genes Dev 17:1557-68. 2003
  35. pmc The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways
    Kimberly L Dodge-Kafka
    Howard Hughes Medical Institute, Oregon Health and Sciences University, Portland, Oregon 97239, USA
    Nature 437:574-8. 2005
    ..Thus, two coupled cAMP-dependent feedback loops are coordinated within the context of the mAKAP complex, suggesting that local control of cAMP signalling by AKAP proteins is more intricate than previously appreciated...
  36. pmc Cardiomyocytes from AKAP7 knockout mice respond normally to adrenergic stimulation
    Brian W Jones
    Department of Pharmacology, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 109:17099-104. 2012
    ..Immunoblot and RT-PCR revealed that only the long isoforms of AKAP7 were detectable in ventricular cardiomyocytes. The results indicate that AKAP7 is not required for regulation of Ca(2+) handling in mouse cardiomyocytes...
  37. pmc MyRIP anchors protein kinase A to the exocyst complex
    April S Goehring
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    J Biol Chem 282:33155-67. 2007
    ..These data indicate that MyRIP functions as a scaffolding protein that links PKA to components of the exocytosis machinery...
  38. ncbi request reprint Orchestration of synaptic plasticity through AKAP signaling complexes
    Andrea L Bauman
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Neuropharmacology 46:299-310. 2004
    ..In this review we will focus on the role of PKA in synaptic plasticity and discuss how the compartmentalization of PKA through its association with A-Kinase Anchoring Proteins (AKAPs) affect PKA function in this process...
  39. pmc AKAP150 Contributes to Enhanced Vascular Tone by Facilitating Large-Conductance Ca2+-Activated K+ Channel Remodeling in Hyperglycemia and Diabetes Mellitus
    Matthew A Nystoriak
    From the Department of Pharmacology, University of California, Davis M A N, M N C, C B N, C Y C, J L P, L T I, D M B, M F N Department of Pharmacology, University of Colorado, Denver M L D Department of Pharmacology, Howard Hughes Medical Institute, University of Washington, Seattle, WA P J N, S A H, J D S and Department of Physiology and Biophysics, University of Washington, Seattle L F S
    Circ Res 114:607-15. 2014
    ..Whether AKAP150 mediates BKCa channel suppression during hyperglycemia and diabetes mellitus is unknown...
  40. ncbi request reprint Calcineurin anchoring and cell signaling
    Kimberly L Dodge
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, Portland, OR, USA
    Biochem Biophys Res Commun 311:1111-5. 2003
    ..This review emphasizes how the targeting of calcineurin through interaction with various anchoring proteins facilitates phosphatase regulation of T-cell activation, neuronal excitability and cardiac hypertrophy...
  41. pmc PP2B/calcineurin-mediated desensitization of TRPV1 does not require AKAP150
    Elaine D Por
    Department of Pharmacology, Center for Biomedical Neuroscience, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229 3900, USA
    Biochem J 432:549-56. 2010
    ..These findings allow us to conclude that pharmacological desensitization of TRPV1 by PP2B may involve additional regulatory components...
  42. pmc Discovery of cellular substrates for protein kinase A using a peptide array screening protocol
    F Donelson Smith
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, 1959 Pacific Avenue NE, Seattle, WA 98195, USA
    Biochem J 438:103-10. 2011
    ....
  43. ncbi request reprint The where's and when's of kinase anchoring
    F Donelson Smith
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, MRB322, 3181 SW Sam Jackson Park Road, Portland, OR 97239, USA
    Trends Biochem Sci 31:316-23. 2006
    ....
  44. pmc A-kinase anchoring proteins take shape
    Darren L Beene
    Howard Hughes Medical Institute, Oregon Health and Science University, 3181 SW Sam Jackson Park Rd, Portland, OR 97239, USA
    Curr Opin Cell Biol 19:192-8. 2007
    ..For example, molecular and genetic approaches have been used to show that the neuronal anchoring protein WAVE1 integrates signaling from PKA and Cdk5 to regulate actin polymerization and cytoskeletal events...
  45. pmc E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis
    Jonathan N Pruneda
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    EMBO J 33:437-49. 2014
    ..Recognizing conserved E2 features, OspG can interact with at least ten distinct human E2s~Ub. Mouse oral infection studies indicate that E2~Ub conjugates act as novel regulators of OspG effector kinase function in eukaryotic host cells. ..
  46. pmc Loss of WAVE-1 causes sensorimotor retardation and reduced learning and memory in mice
    Scott H Soderling
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA
    Proc Natl Acad Sci U S A 100:1723-8. 2003
    ..Thus WAVE-1 is required for normal neural functioning...
  47. pmc Chronic liver disease in Aboriginal North Americans
    John D Scott
    Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Harborview Medical Center, 325 Ninth Avenue, Seattle, WA 98144, USA
    World J Gastroenterol 14:4607-15. 2008
    ..In addition, more research is needed on the treatment of hepatitis C virus (HCV) infection and non-alcoholic fatty liver disease (NAFLD) in this population...
  48. ncbi request reprint Influenza A pneumonia presenting as progressive focal infiltrates in a stem cell transplant recipient
    John D Scott
    Department of Medicine, University of Washington, Harborview Medical Center, 325 Ninth Avenue, Box 359938, Seattle, WA 98104 2499, USA
    J Clin Virol 31:96-9. 2004
    ..Stem cell transplant recipients are susceptible to pulmonary infections, including influenza A. Typically, isolated influenza pneumonia has a diffuse, interstitial infiltrate pattern...
  49. ncbi request reprint High rate of spontaneous negativity for hepatitis C virus RNA after establishment of chronic infection in Alaska Natives
    John D Scott
    Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA
    Clin Infect Dis 42:945-52. 2006
    ..Hepatitis C virus (HCV) leads to chronic infection in 70%-85% of exposed patients. Spontaneous clearance of the virus after chronic infection is believed to occur rarely...
  50. doi request reprint Diagnosis of depression in former injection drug users with chronic hepatitis C
    John D Scott
    Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, WA 98104, USA
    J Clin Gastroenterol 45:462-7. 2011
    ..This study seeks to define the performance characteristics of 2 common depression screening tests and how psychiatric diagnoses affect adherence to treatment...
  51. ncbi request reprint Role of combination therapy in chronic hepatitis B
    John D Scott
    Harborview Medical Center, 325 Ninth Avenue, Box 359938, Seattle, WA 98104, USA
    Curr Gastroenterol Rep 11:28-36. 2009
    ....
  52. pmc Hepatitis C virus is infrequently evaluated and treated in an urban HIV clinic population
    John D Scott
    Department of Medicine, Division of Allergy and Infectious Diseases, University of Washington, Seattle, Washington, USA
    AIDS Patient Care STDS 23:925-9. 2009
    ..Further efforts to identify and to overcome barriers to HCV treatment are warranted...
  53. pmc Scaffolding proteins: not such innocent bystanders
    F Donelson Smith
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington, Seattle, WA 98195, USA
    Curr Biol 23:R515-7. 2013
    ..Though frequently considered to be inert organizational elements, two recent reports implicate kinase-scaffolding proteins as active participants in signal relay. ..
  54. pmc Local control of TRPV4 channels by AKAP150-targeted PKC in arterial smooth muscle
    Jose Mercado
    Department of Physiology and Biophysics and 2 Howard Hughes Medical Institute, Department of Pharmacology, University of Washington, Seattle, WA 98195
    J Gen Physiol 143:559-75. 2014
    ..We propose that AKAP150, PKC, and TRPV4 channels form dynamic subcellular signaling domains that control Ca(2+) influx into arterial myocytes...
  55. pmc AKAP-anchored PKA maintains neuronal L-type calcium channel activity and NFAT transcriptional signaling
    Jonathan G Murphy
    Department of Pharmacology, Anschutz Medical Campus, University of Colorado School of Medicine, Aurora, CO 80045, USA Neuroscience Program, Anschutz Medical Campus, University of Colorado School of Medicine, Aurora, CO 80045, USA
    Cell Rep 7:1577-88. 2014
    ..Thus, LTCC-NFAT transcriptional signaling in neurons requires precise organization and balancing of PKA and CaN activities in the channel nanoenvironment, which is only made possible by AKAP79/150 scaffolding...
  56. pmc Creating order from chaos: cellular regulation by kinase anchoring
    John D Scott
    Howard Hughes Medical Institute and Department of Pharmacology, University of Washington School of Medicine, Seattle, Washington 98195, USA
    Annu Rev Pharmacol Toxicol 53:187-210. 2013
    ....
  57. ncbi request reprint Kinase- and phosphatase-anchoring proteins: harnessing the dynamic duo
    Andrea L Bauman
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, 3181 S W Sam Jackson Park Road, Portland, OR 97201 3098, USA
    Nat Cell Biol 4:E203-6. 2002
  58. ncbi request reprint The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling
    Scott H Soderling
    Howard Hughes Medical Institute, Vollum Institute, 3181 Sam Jackson Park Road, Portland, Oregon 97239 3098, USA
    Nat Cell Biol 4:970-5. 2002
    ..WRP binds directly to WAVE-1 through its Src homology domain 3 and specifically inhibits Rac function in vivo. Thus, we propose that WRP is a binding partner of WAVE-1 that functions as a signal termination factor for Rac...
  59. ncbi request reprint A-kinase-anchoring proteins
    Lorene K Langeberg
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, Portland, OR 97239, USA
    J Cell Sci 118:3217-20. 2005
  60. ncbi request reprint AKAP mediated signal transduction
    Jennifer J Carlisle Michel
    Howard Hughes Medical Institute, Vollum Institute, Portland, Oregon 97201 3098, USA
    Annu Rev Pharmacol Toxicol 42:235-57. 2002
    ..This review details the most recent advances in our understanding of the various biological functions dependent upon AKAP-anchored signaling complexes...
  61. ncbi request reprint AKAP150-dependent cooperative TRPV4 channel gating is central to endothelium-dependent vasodilation and is disrupted in hypertension
    Swapnil K Sonkusare
    Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05403, USA
    Sci Signal 7:ra66. 2014
    ..Disruption of this molecular assembly may contribute to altered blood flow in hypertension. ..
  62. pmc Alpha-dystrobrevin-1 recruits alpha-catulin to the alpha1D-adrenergic receptor/dystrophin-associated protein complex signalosome
    John S Lyssand
    Department of Pharmacology and Physiology and Biophysics, University of Washington, Seattle, WA 98195, USA
    Proc Natl Acad Sci U S A 107:21854-9. 2010
    ..Taken together, our study implicates α-catulin as a unique regulator of GPCR signaling and represents a unique expansion of the intricate and continually evolving array of GPCR signaling networks...
  63. pmc Signal integration through blending, bolstering and bifurcating of intracellular information
    Catherine T Pawson
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington, School of Medicine, Seattle, Washington, USA
    Nat Struct Mol Biol 17:653-8. 2010
    ..The ability to organize these enzymes into multiprotein complexes allows for a high degree of fidelity, efficiency and spatial precision in signaling responses...
  64. pmc Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics
    Neal M Alto
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201, USA
    J Cell Biol 158:659-68. 2002
    ..This implicates Rab32 as a participant in synchronization of mitochondrial fission. Thus, Rab32 is a dual function protein that participates in both mitochondrial anchoring of PKA and mitochondrial dynamics...
  65. ncbi request reprint Signaling complexes: junctions on the intracellular information super highway
    F Donelson Smith
    Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Sciences University, 3181 S W Sam Jackson Park Road, Portland, Oregon 97201 3098, USA
    Curr Biol 12:R32-40. 2002
    ..A major objective of the signaling community is to ascertain how signals flow through compartmentalized transduction units that contain transmembrane receptors, protein kinases, phosphatases and their substrates...
  66. pmc Project ECHO: a model for complex, chronic care in the Pacific Northwest region of the United States
    John D Scott
    School of Medicine, University of Washington, Seattle, WA 98104, USA
    J Telemed Telecare 18:481-4. 2012
    ..The Project ECHO model improves access to health care. It may provide a way to bring specialist care to rural areas in developing countries...
  67. pmc AKAP signaling complexes: pointing towards the next generation of therapeutic targets?
    Jessica L Esseltine
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle, WA, USA
    Trends Pharmacol Sci 34:648-55. 2013
    ..This review will highlight recent advances in AKAP research focusing on local signaling events that are perturbed in disease. ..
  68. pmc Allele-specific PCR for determination of IL28B genotype
    Linda Cook
    Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA
    J Clin Microbiol 50:4144-6. 2012
    ..We describe an allele-specific PCR assay for the IL28B genotype. The assay is simple and robust, uses commonly available real-time PCR instrumentation, and is well suited for clinical laboratories offering IL28B genotyping...
  69. pmc Networking with AKAPs: context-dependent regulation of anchored enzymes
    Emily J Welch
    Howard Hughes Medical Institute, Department of Pharmacology, University of Washington School of Medicine, Seattle, WA 98195, USA
    Mol Interv 10:86-97. 2010
    ..This article charts the inception of the PKA-anchoring hypothesis, the characterization of AKAPs and their nomenclature, and the physiological roles of context-specific AKAP signaling complexes...

Research Grants16

  1. Anchored Kinase Signaling Mechanisms in Cardiac Hypertrophy
    John D Scott; Fiscal Year: 2010
    ....
  2. Anchored Kinase Signaling Mechanisms in Cardiac Hypertrophy
    John D Scott; Fiscal Year: 2010
    ....
  3. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 1999
    ....
  4. Anchored Kinase Signaling Mechanisms in Cardiac Hypertrophy
    John Scott; Fiscal Year: 2009
    ....
  5. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 2003
    ....
  6. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 2002
    ....
  7. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 2001
    ....
  8. MOLECULAR ANALYSIS OF ANCHORING PROTEINS
    John Scott; Fiscal Year: 2000
    ....
  9. MOLECULAR ANALYSIS OF PKA-ANCHORING PROTEIN INTERACTION
    John Scott; Fiscal Year: 1993
    ..Therefore, specific aim 2 will use recombinant RIalpha as a probe to screen a pituitary cDNA expression library to isolate clones to this RI-anchoring protein...
  10. MOLECULAR ANALYSIS OF PKA-ANCHORING PROTEIN INTERACTION
    John Scott; Fiscal Year: 1992
    ..Therefore, specific aim 2 will use recombinant RIalpha as a probe to screen a pituitary cDNA expression library to isolate clones to this RI-anchoring protein...