E E Scott

Summary

Affiliation: University of Texas Medical Branch
Country: USA

Publications

  1. ncbi request reprint A truncation of 2B subfamily cytochromes P450 yields increased expression levels, increased solubility, and decreased aggregation while retaining function
    E E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, 77555 1031, USA
    Arch Biochem Biophys 395:57-68. 2001
  2. ncbi request reprint Substrate routes to the buried active site may vary among cytochromes P450: mutagenesis of the F-G region in P450 2B1
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555, USA
    Chem Res Toxicol 15:1407-13. 2002
  3. ncbi request reprint Mutagenesis and molecular dynamics suggest structural and functional roles for residues in the N-terminal portion of the cytochrome P450 2B1 I helix
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
    Arch Biochem Biophys 423:266-76. 2004
  4. ncbi request reprint The role of cytochrome 2B1 substrate recognition site residues 115, 294, 297, 298, and 362 in the oxidation of steroids and 7-alkoxycoumarins
    T L Domanski
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Arch Biochem Biophys 394:21-8. 2001
  5. pmc p-dimethylaminocinnamaldehyde derivatization for colorimetric detection and HPLC-UV/vis-MS/MS identification of indoles
    Patrick R Porubsky
    Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Dr, Lawrence, KS 66045, USA
    Arch Biochem Biophys 475:14-7. 2008
  6. ncbi request reprint Structure of the human lung cytochrome P450 2A13
    Brian D Smith
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 282:17306-13. 2007
  7. ncbi request reprint Possible pathway(s) of testosterone egress from the active site of cytochrome P450 2B1: a steered molecular dynamics simulation
    Weihua Li
    Center for Drug Discovery and Design, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
    Drug Metab Dispos 33:910-9. 2005
  8. ncbi request reprint Functional role of residues in the helix B' region of cytochrome P450 2B1
    Wataru Honma
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
    Arch Biochem Biophys 435:157-65. 2005
  9. ncbi request reprint Structures of cytochrome P450 3A4
    Emily E Scott
    Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045 7582, USA
    Trends Biochem Sci 30:5-7. 2005
  10. ncbi request reprint Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9-A resolution: insight into the range of P450 conformations and the coordination of redox partner binding
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555 1031, USA
    J Biol Chem 279:27294-301. 2004

Collaborators

Detail Information

Publications14

  1. ncbi request reprint A truncation of 2B subfamily cytochromes P450 yields increased expression levels, increased solubility, and decreased aggregation while retaining function
    E E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas, 77555 1031, USA
    Arch Biochem Biophys 395:57-68. 2001
    ..These data demonstrate that modification of the N-terminus yields high levels of properly folded P450s 2B with increased solubility, which are suitable for functional and structural analysis...
  2. ncbi request reprint Substrate routes to the buried active site may vary among cytochromes P450: mutagenesis of the F-G region in P450 2B1
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555, USA
    Chem Res Toxicol 15:1407-13. 2002
    ..The minimal changes in 2B1 do not support access via the F-G region of 2B1 and suggest the alternate access route identified in P450 51...
  3. ncbi request reprint Mutagenesis and molecular dynamics suggest structural and functional roles for residues in the N-terminal portion of the cytochrome P450 2B1 I helix
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
    Arch Biochem Biophys 423:266-76. 2004
    ..Sensitivity of holoprotein formation to substitution and effects on substrate binding and metabolism suggest structural and functional roles for residues in the N-terminus of the cytochrome P450 2B1 I helix...
  4. ncbi request reprint The role of cytochrome 2B1 substrate recognition site residues 115, 294, 297, 298, and 362 in the oxidation of steroids and 7-alkoxycoumarins
    T L Domanski
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas 77555, USA
    Arch Biochem Biophys 394:21-8. 2001
    ..The results from this study illustrate the conservation of functionally important residues across P450 subfamilies and families...
  5. pmc p-dimethylaminocinnamaldehyde derivatization for colorimetric detection and HPLC-UV/vis-MS/MS identification of indoles
    Patrick R Porubsky
    Department of Medicinal Chemistry, University of Kansas, 1251 Wescoe Hall Dr, Lawrence, KS 66045, USA
    Arch Biochem Biophys 475:14-7. 2008
    ..The ligand in the crystallized protein was identified as unsubstituted indole, which facilitated refinement of two alternate conformations in the CYP2A13 crystal structure active site...
  6. ncbi request reprint Structure of the human lung cytochrome P450 2A13
    Brian D Smith
    Department of Medicinal Chemistry, University of Kansas, Lawrence, Kansas 66045, USA
    J Biol Chem 282:17306-13. 2007
    ..In addition, docking studies suggest that residues 365 and 366 may also contribute to differences in NNK metabolism...
  7. ncbi request reprint Possible pathway(s) of testosterone egress from the active site of cytochrome P450 2B1: a steered molecular dynamics simulation
    Weihua Li
    Center for Drug Discovery and Design, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
    Drug Metab Dispos 33:910-9. 2005
    ..Phe115 acts as a gatekeeper for channel 2. These results are in agreement with previous site-directed mutagenesis experiments...
  8. ncbi request reprint Functional role of residues in the helix B' region of cytochrome P450 2B1
    Wataru Honma
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555 1031, USA
    Arch Biochem Biophys 435:157-65. 2005
    ..The findings suggest that residues in the helix B' region affect regio- and stereoselective oxidation in P450 family 2 enzymes as well as substrate entry...
  9. ncbi request reprint Structures of cytochrome P450 3A4
    Emily E Scott
    Department of Medicinal Chemistry, University of Kansas, Lawrence, KS 66045 7582, USA
    Trends Biochem Sci 30:5-7. 2005
    ....
  10. ncbi request reprint Structure of mammalian cytochrome P450 2B4 complexed with 4-(4-chlorophenyl)imidazole at 1.9-A resolution: insight into the range of P450 conformations and the coordination of redox partner binding
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston 77555 1031, USA
    J Biol Chem 279:27294-301. 2004
    ..Comparison of the 2B4/CPI complex with the open 2B4 structure yields insights into the dynamics involved in substrate access, tight inhibitor binding, and coordination of substrate and redox partner binding...
  11. pmc An open conformation of mammalian cytochrome P450 2B4 at 1.6-A resolution
    Emily E Scott
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, TX 77555 1031, USA
    Proc Natl Acad Sci U S A 100:13196-201. 2003
    ..This conformational flexibility is likely to facilitate substrate access, metabolic versatility, and product egress...
  12. ncbi request reprint A rational approach to Re-engineer cytochrome P450 2B1 regioselectivity based on the crystal structure of cytochrome P450 2C5
    Santosh Kumar
    Department of Pharmacology and Toxicology, University of Texas Medical Branch, Galveston, Texas 77555 1031, USA
    J Biol Chem 278:17178-84. 2003
    ....
  13. pmc Expression, purification, crystallization and preliminary X-ray studies of histamine dehydrogenase from Nocardioides simplex
    Timothy M Reed
    Department of Chemistry, The University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:785-7. 2008
    ..Diffraction data were collected to 2.7 A resolution at the SSRL synchrotron with 99.7% completeness. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 101.14, b = 107.03, c = 153.35 A...
  14. pmc Expression, purification, crystallization and preliminary X-ray studies of a prolyl-4-hydroxylase protein from Bacillus anthracis
    Megen A Miller
    Department of Chemistry, The University of Kansas, 1251 Wescoe Hall Drive, Lawrence, KS 66045, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:788-91. 2008
    ..X-ray diffraction data of selenomethionine-labeled anthrax-P4H recombinantly expressed in Escherichia coli have been collected to 1.4 A resolution...