R Scofield

Summary

Affiliation: University of Oklahoma Health Sciences Center
Country: USA

Publications

  1. doi request reprint Juvenile spondyloarthropathies
    R Hal Scofield
    Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation RHS, ALS, Oklahoma City, OK, USA
    Curr Rheumatol Rep 14:395-401. 2012
  2. doi request reprint Mapping susceptibility gene in systemic lupus erythematosus
    R Hal Scofield
    Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Methods Mol Biol 900:11-24. 2012
  3. pmc IL-21 and Sjögren's syndrome
    R Hal Scofield
    Departments of Medicine and Pathology, University of Oklahoma Health Sciences Center, 1000 North Lincoln Blvd, Oklahoma City, OK 73104, USA
    Arthritis Res Ther 13:137. 2011
  4. pmc Klinefelter's syndrome (47,XXY) in male systemic lupus erythematosus patients: support for the notion of a gene-dose effect from the X chromosome
    R Hal Scofield
    Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, and Oklahoma City VAMC, Oklahoma City, OK 73104, USA
    Arthritis Rheum 58:2511-7. 2008
  5. ncbi request reprint Genetic knock out of 60kD Ro (or SSA), a common lupus autoantigen, induces lupus
    R Hal Scofield
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Trends Immunol 25:1-3. 2004
  6. pmc Autoimmune thyroid disease is associated with a diagnosis of secondary Sjögren's syndrome in familial systemic lupus
    R Hal Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Ann Rheum Dis 66:410-3. 2007
  7. ncbi request reprint Autoantibodies as predictors of disease
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Lancet 363:1544-6. 2004
  8. ncbi request reprint Immunization with short peptides from the 60-kDa Ro antigen recapitulates the serological and pathological findings as well as the salivary gland dysfunction of Sjogren's syndrome
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    J Immunol 175:8409-14. 2005
  9. ncbi request reprint Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoprotein
    R H Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, The Veterans Affairs Medical Center, and the W K Warren Medical Research Institute, Oklahoma City, USA
    J Rheumatol 27:128-34. 2000
  10. doi request reprint Genetics of systemic lupus erythematosus and Sjögren's syndrome
    R Hal Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    Curr Opin Rheumatol 21:448-53. 2009

Detail Information

Publications74

  1. doi request reprint Juvenile spondyloarthropathies
    R Hal Scofield
    Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation RHS, ALS, Oklahoma City, OK, USA
    Curr Rheumatol Rep 14:395-401. 2012
    ..These agents also have serious complications, including induction of other autoimmune diseases...
  2. doi request reprint Mapping susceptibility gene in systemic lupus erythematosus
    R Hal Scofield
    Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Methods Mol Biol 900:11-24. 2012
    ..This effort can also be used to identify causal alleles from association intervals not yet otherwise identified...
  3. pmc IL-21 and Sjögren's syndrome
    R Hal Scofield
    Departments of Medicine and Pathology, University of Oklahoma Health Sciences Center, 1000 North Lincoln Blvd, Oklahoma City, OK 73104, USA
    Arthritis Res Ther 13:137. 2011
    ..Demonstration of IL-21 as a fundamental aspect of the pathophysiology of Sjögren's syndrome could lead to the development of anti-IL-21 therapy for this disease...
  4. pmc Klinefelter's syndrome (47,XXY) in male systemic lupus erythematosus patients: support for the notion of a gene-dose effect from the X chromosome
    R Hal Scofield
    Oklahoma Medical Research Foundation, University of Oklahoma Health Sciences Center, and Oklahoma City VAMC, Oklahoma City, OK 73104, USA
    Arthritis Rheum 58:2511-7. 2008
    ..The present study was undertaken to investigate the prevalence of Klinefelter's syndrome in a large population of patients with SLE...
  5. ncbi request reprint Genetic knock out of 60kD Ro (or SSA), a common lupus autoantigen, induces lupus
    R Hal Scofield
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Trends Immunol 25:1-3. 2004
    ..These data add to the expanding cooperation between mouse and human SLE genetic studies and are the first in which ablation of an autoantigen induces an autoimmune disease...
  6. pmc Autoimmune thyroid disease is associated with a diagnosis of secondary Sjögren's syndrome in familial systemic lupus
    R Hal Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Ann Rheum Dis 66:410-3. 2007
    ..Autoimmune thyroid disease is common in systemic lupus erythematosus (SLE). About 20% of patients with SLE have secondary Sjögren's syndrome...
  7. ncbi request reprint Autoantibodies as predictors of disease
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Lancet 363:1544-6. 2004
    ..Furthermore, for many diseases these autoantibodies are found in serum samples many years before disease onset...
  8. ncbi request reprint Immunization with short peptides from the 60-kDa Ro antigen recapitulates the serological and pathological findings as well as the salivary gland dysfunction of Sjogren's syndrome
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    J Immunol 175:8409-14. 2005
    ..BALB/c mice immunized with 60-kDa Ro peptides develop anti-Ro, salivary gland lymphocyte infiltrates, and salivary dysfunction that is highly reminiscent of human Sjögren's syndrome...
  9. ncbi request reprint Anti-calreticulin segregates anti-Ro sera in systemic lupus erythematosus: anti-calreticulin is present in sera with anti-Ro alone but not in anti-Ro sera with anti-La or anti-ribonucleoprotein
    R H Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, The Veterans Affairs Medical Center, and the W K Warren Medical Research Institute, Oklahoma City, USA
    J Rheumatol 27:128-34. 2000
    ..To examine a well characterized group of patients with systemic lupus erythematosus (SLE) for anti-calreticulin...
  10. doi request reprint Genetics of systemic lupus erythematosus and Sjögren's syndrome
    R Hal Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    Curr Opin Rheumatol 21:448-53. 2009
    ..To determine the advances made in the genetics of systemic lupus erythematosus (SLE) or Sjögren's syndrome as the era of genome-wide association and high-throughput single nucleotide typing begins...
  11. ncbi request reprint Immunization with peptides from 60 kDa Ro in diverse mouse strains
    R H Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Department of Medicine, University of Oklahoma Health Sciences Center and the Department of Veterans Affairs Medical Center, Oklahoma City, OK 73105, USA
    Scand J Immunol 56:477-83. 2002
    ..Furthermore, these data suggest that genetic variation is important with regard to responding towards short peptide immunization by epitope spreading...
  12. ncbi request reprint Thrombocytopenia identifies a severe familial phenotype of systemic lupus erythematosus and reveals genetic linkages at 1q22 and 11p13
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, USA
    Blood 101:992-7. 2003
    ....
  13. doi request reprint Vasculitis in Sjögren's Syndrome
    R Hal Scofield
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, USA
    Curr Rheumatol Rep 13:482-8. 2011
    ..Devic disease patients have salivary lymphocytic infiltration similar to that found among Sjögren's syndrome patients...
  14. ncbi request reprint Can B cell epitopes of 60 kDa Ro distinguish systemic lupus erythematosus from Sjögren's syndrome?
    A N Scofield
    Oklahoma Medical Research Program, University of Oklahoma Health Sciences Center, USA
    Lupus 10:547-53. 2001
    ..Thus, the fine specificity of antibody binding to 60 kDa Ro varies according to the presence of anti-La, not to the diagnosis of either SLE or SS...
  15. ncbi request reprint Protein-protein interaction of the Ro-ribonucleoprotein particle using multiple antigenic peptides
    R H Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    Mol Immunol 36:1093-106. 1999
    ..The present data indicate that there are protein-protein interactions between Ro and La...
  16. ncbi request reprint Modification of lupus-associated 60-kDa Ro protein with the lipid oxidation product 4-hydroxy-2-nonenal increases antigenicity and facilitates epitope spreading
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Free Radic Biol Med 38:719-28. 2005
    ..These data may suggest a hitherto unappreciated mechanism by which oxidative stress facilitates epitope spreading in SLE...
  17. pmc Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: a possible therapy to alleviate autoimmune disorders
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Mol Nutr Food Res 54:1202-9. 2010
    ..In addition, the non-toxic curcumin could serve as a new protein stain in SDS-PAGE even though it is less sensitive than the Coomassie system which involves toxic chemicals...
  18. ncbi request reprint Interaction of calcium and Ro60: increase of antigenicity
    R Hal Scofield
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation OMRF, 825 NE 13th Street, MS 38, Oklahoma City, OK 73104, USA
    Mol Immunol 41:809-16. 2004
    ..Thus, the observed intramolecular and intermolecular interactions and antigen-antibody interactions could be Ca2+ ion mediated conformational interactions, and we propose that 60 kDa Ro is a calcium binding protein...
  19. doi request reprint Affinity immunoblotting for analysis of antibody clonotype distribution in a lupus patient developing anti-Ro 60 over time
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
    Methods Mol Biol 536:45-53. 2009
    ..The bound IgG clonotypes were detected using alkaline phosphatase conjugated anti-IgG. The patient's sera demonstrated an oligoclonal response to the Ro 60 autoantigen that increased in complexity and affinity over time...
  20. pmc Autoimmunity and oxidatively modified autoantigens
    Biji T Kurien
    Arthritis and Immunology Program, USA
    Autoimmun Rev 7:567-73. 2008
    ..However, this should be viewed in the light of disappointing results obtained with the use of antioxidants in cardiovascular disease...
  21. ncbi request reprint Development of autoantibodies before the clinical onset of systemic lupus erythematosus
    Melissa R Arbuckle
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    N Engl J Med 349:1526-33. 2003
    ..We investigated the onset and progression of autoantibody development before the clinical diagnosis...
  22. doi request reprint Ultrarapid electrophoretic transfer of high and low molecular weight proteins using heat
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, OKC, OK, 73104, USA
    Methods Mol Biol 536:181-90. 2009
    ..In summary, this procedure is particularly useful for the transfer of high molecular weight proteins, very rapid, and avoids the use of methanol...
  23. pmc Detection of catalase as a major protein target of the lipid peroxidation product 4-HNE and the lack of its genetic association as a risk factor in SLE
    Anil D'Souza
    Department of Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA
    BMC Med Genet 9:62. 2008
    ..Hence we investigated the effects of a genetic polymorphism of catalase on risk and severity of SLE in 48 pedigrees with African American ancestry...
  24. pmc High-density genotyping of STAT4 reveals multiple haplotypic associations with systemic lupus erythematosus in different racial groups
    Bahram Namjou
    Oklahoma Medical Research Foundation, Oklahoma City, USA
    Arthritis Rheum 60:1085-95. 2009
    ..STAT-1 and STAT-4 are transcription factors that play key roles in the interferon and Th1 signaling pathways, making them attractive candidates for involvement in SLE susceptibility...
  25. doi request reprint Detection of La/SS-B by western blot using nanogold-tagged antibodies and silver enhancement
    Shannon Maier
    Department of Arthritis and Immunology, Oklahoma Medical Research Foundation, MS 24, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:491-7. 2009
    ....
  26. pmc Barbering in mice: a model for trichotillomania
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    BMJ 331:1503-5. 2005
  27. pmc Common variants within MECP2 confer risk of systemic lupus erythematosus
    Amr H Sawalha
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    PLoS ONE 3:e1727. 2008
    ..These findings are potentially related to the overexpression of methylation-sensitive genes in SLE...
  28. ncbi request reprint Presence of anti-La autoantibody is associated with a lower risk of nephritis and seizures in lupus patients
    S Malik
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA
    Lupus 16:863-6. 2007
    ..3% versus 27.4%, OR = 0.31, P = 0.0075). In conclusion, anti-La autoantibody is associated with less severe lupus. Patients with anti-La have a lower risk of renal involvement and seizures compared with anti-La negative lupus patients...
  29. doi request reprint Nonelectrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple antigens to obtain 12 immunoblots
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:55-65. 2009
    ....
  30. ncbi request reprint Free radical mediated peroxidative damage in systemic lupus erythematosus
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    Life Sci 73:1655-66. 2003
    ..Heat inactivated anti-SOD autoantibodies were able to inhibit the activity of the enzyme. We propose that the inhibition of SOD by autoantibodies is, in part, responsible for the increased free radical damage seen in the disease...
  31. doi request reprint A brief review of other notable protein blotting methods
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:367-84. 2009
    ..All these methods are briefly reviewed in this chapter...
  32. doi request reprint A brief review of other notable protein detection methods on blots
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
    Methods Mol Biol 536:557-71. 2009
    ..These methods are briefly reviewed in this chapter...
  33. pmc Autoimmunity and Klinefelter's syndrome: when men have two X chromosomes
    Amr H Sawalha
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    J Autoimmun 33:31-4. 2009
    ..On the other hand, a gene dose effect could also be mediated by a gene without an SLE-associated polymorphism in that a gene that avoids X inactivation will have a higher level of expression in persons with two X chromosomes...
  34. ncbi request reprint Curcumin/turmeric solubilized in sodium hydroxide inhibits HNE protein modification--an in vitro study
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma City, OK 73104, USA
    J Ethnopharmacol 110:368-73. 2007
    ..The pH at which the inhibition of HNE modification of substrate was observed was close to the physiological pH, making this formulation of curcumin potentially useful practically...
  35. pmc Male-only systemic lupus
    Rachna Aggarwal
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Rheumatol 37:1480-7. 2010
    ..Systemic lupus erythematosus (SLE) is more common among women than men, a ratio of about 10 to 1. We undertook this study to describe familial male SLE within a large familial SLE cohort...
  36. doi request reprint An overview of Western blotting for determining antibody specificities for immunohistochemistry
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Methods Mol Biol 717:55-67. 2011
    ..Specificity of antibodies used for immunohistochemistry is of critical importance and therefore Western blot is a "must" to address antibodies' specificity...
  37. ncbi request reprint Heat-mediated, ultra-rapid electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    J Immunol Methods 266:127-33. 2002
    ..In summary, this procedure is very rapid, avoids the use of methanol and is particularly useful for the transfer of high molecular weight proteins...
  38. ncbi request reprint Extraction of nucleic acid fragments from gels
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, 73104, USA
    Anal Biochem 302:1-9. 2002
  39. ncbi request reprint Improving the solubility and pharmacological efficacy of curcumin by heat treatment
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Assay Drug Dev Technol 5:567-76. 2007
    ..Heat-solubilized curcumin should be considered in clinical trials involving curcumin, especially in the face of frustrating results obtained regarding curcumin-mediated correction of cystic fibrosis defects...
  40. ncbi request reprint Can low dose diagnostic dental radiation trigger Sjögren's syndrome?
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Med Hypotheses 69:995-1000. 2007
    ..We hypothesize that diagnostic dental radiation could be a factor for the development of SS as a result of either radiation induced apoptosis or through oxidative modification of proteins...
  41. ncbi request reprint Western blotting
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
    Methods 38:283-93. 2006
    ..Finally, a brief description of methods generally used to detect antigens on blots is also described...
  42. ncbi request reprint Determination of prolidase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Anal Biochem 331:224-9. 2004
    ..Using this method we have unambiguously identified subjects with homozygous or heterozygous prolidase deficiency. In addition to the advantage of rapid sample preparation time, this method is highly specific, reproducible, and sensitive...
  43. ncbi request reprint Prolidase deficiency and the biochemical assays used in its diagnosis
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Anal Biochem 349:165-75. 2006
  44. ncbi request reprint Linkage at 5q14.3-15 in multiplex systemic lupus erythematosus pedigrees stratified by autoimmune thyroid disease
    Bahram Namjou
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Arthritis Rheum 52:3646-50. 2005
    ..To identify genetic effects potentially shared between systemic lupus erythematosus (SLE) and autoimmune thyroiditis (AITD)...
  45. ncbi request reprint In vitro modification of solid phase multiple antigenic peptides/autoantigens with 4-hydroxy-2-nonenal (HNE) provide ideal substrates for detection of anti-HNE antibodies and peptide antioxidants
    Biji T Kurien
    Arthritis and Immunology Program, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    J Immunol Methods 303:66-75. 2005
    ..Glycyl-proline dipeptide and a MAP from the Sm autoantigen (PPPGMRPP) showed 0% inhibition of HNE-modification...
  46. ncbi request reprint Protein blotting: a review
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    J Immunol Methods 274:1-15. 2003
  47. ncbi request reprint Experimental animal urine collection: a review
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, OK 73104, USA
    Lab Anim 38:333-61. 2004
    ..A continuous refinement in the procedures for collecting urine from experimental animals will be the most efficient way of proceeding in obtaining pure urine specimens for obtaining reliable research data...
  48. ncbi request reprint Mechanized syringe homogenization of human and animal tissues
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 825 NE 13th Street, OK 73104, USA
    Assay Drug Dev Technol 2:308-12. 2004
    ..Use of the Bio-Mixer 1200 to homogenize animal or human tissue precludes the need to stay in the cold room as is the case with the other hands-on homogenization methods available, in addition to freeing up time for other experiments...
  49. ncbi request reprint Lipid peroxidation in systemic lupus erythematosus
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, USA
    Indian J Exp Biol 44:349-56. 2006
    ..Immunization with an oxidatively modified autoantigen led to the rapid development of autoimmunity compared to immunization with the unmodified autoantigen. Thus, oxidative damage appears to play an important role in SLE pathogenesis...
  50. ncbi request reprint Epstein-Barr virus and systemic lupus erythematosus
    Judith A James
    Arthritis and Immunology, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Curr Opin Rheumatol 18:462-7. 2006
    ..Although many genetic and environmental factors are associated, this review will focus on the evolving evidence for key Epstein-Barr virus specific roles...
  51. ncbi request reprint A candidate region on 11p13 for systemic lupus erythematosus: a linkage identified in African-American families
    Swapan K Nath
    Genetic Epidemiology Unit, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA
    J Investig Dermatol Symp Proc 9:64-7. 2004
    ....
  52. ncbi request reprint Neonatal lupus manifests as isolated neutropenia and mildly abnormal liver functions
    Sivalingam Kanagasegar
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
    J Rheumatol 29:187-91. 2002
    ..Furthermore, although other factors may contribute, these data suggest that anti-60 kDa Ro is directly involved in the pathogenesis of neutropenia...
  53. doi request reprint Tryptic peptide purification using polyvinylidene difluoride membrane for matrix-assisted laser desorption ionization time of flight mass spectrometry
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:39-44. 2009
    ..Using this procedure, we were able to identify a cross-reactive D1 autoantigen on the surface of neutrophils that bound antibodies targeting Ro 60 autoantigen in systemic lupus erythematosus...
  54. pmc Problems with multiple use of transfer buffer in protein electrophoretic transfer
    Yaser Dorri
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma, USA
    J Biomol Tech 21:1-2. 2010
    ..Here, we comment on this report, as we believe this method is not accurate and useful for protein analysis, and it can cause background binding as well as inaccurate protein analysis...
  55. doi request reprint Whole and strip nitrocellulose membrane as well as a new-line-immunoblotting of antigen using the chemiluminescence technique
    Yaser Dorri
    Oklahoma Medical Research Foundation, 825 NE 13th St, Arthritis and Immunology Department, MS 24, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:417-32. 2009
    ....
  56. pmc Evidence for a susceptibility gene (SLEH1) on chromosome 11q14 for systemic lupus erythematosus (SLE) families with hemolytic anemia
    Jennifer A Kelly
    Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 99:11766-71. 2002
    ..Thus, we show strong evidence for an SLE susceptibility gene, SLEH1, near D11S2002 in African-American pedigrees multiplex for SLE that have at least one SLE-affected patient with hemolytic anemia...
  57. ncbi request reprint Epstein-Barr virus and molecular mimicry in systemic lupus erythematosus
    Brian D Poole
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma, OK 73104, USA
    Autoimmunity 39:63-70. 2006
    ..This paper reviews some of the current literature supporting roles for EBV exposure and epitope spreading in SLE...
  58. doi request reprint Protein stains to detect antigen on membranes
    Anil D'Souza
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:433-40. 2009
    ..Detection is often synonymous to staining, i.e., the reversible or irreversible binding by the proteins of a colored organic or inorganic chemical...
  59. pmc Inhalation of environmental stressors & chronic inflammation: autoimmunity and neurodegeneration
    Sandra E Gomez-Mejiba
    Free Radical Biology and Aging Research Program, MS 21, 825 NE 13th Street, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, United States
    Mutat Res 674:62-72. 2009
    ....
  60. doi request reprint Introduction to protein blotting
    Biji T Kurien
    Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA
    Methods Mol Biol 536:9-22. 2009
    ..The scientific community is now confronted with a variety of ways and means to carry out this transfer...
  61. ncbi request reprint Oxidatively modified autoantigens in autoimmune diseases
    Biji T Kurien
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, OK 73104, USA
    Free Radic Biol Med 41:549-56. 2006
    ....
  62. ncbi request reprint Horizons in Sjögren's syndrome genetics
    Pamela H Williams
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA
    Clin Rev Allergy Immunol 32:201-9. 2007
    ..Newly developed genomic capacity will provide, in the future, a much closer approximation of the true picture of the genetic architecture of Sjögren's syndrome...
  63. ncbi request reprint Mapping the systematic lupus erythematosus susceptibility genes
    Swapan K Nath
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA
    Methods Mol Med 102:11-29. 2004
    ..SLE is a complex clinical illness, and investigation of the genetics of the illness based on clinical manifestations revealed linkages not found without consideration of the phenotype of the disease...
  64. doi request reprint Phosphoprotein detection on protein electroblot using a phosphate-specific fluorophore
    Lee Broderick Bockus
    Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:385-93. 2009
    ..It can also be followed by standard chemiluminescent, colorimetric, and fluorogenic detection techniques used in immunoblotting...
  65. doi request reprint Grid-immunoblotting
    Olga Yeliosof
    Oklahoma Medical Research Foundation, 825 NE 13th Street Mailstop 24, Oklahoma City, OK, 73104, USA
    Methods Mol Biol 536:289-92. 2009
    ..Grid-Immunoblotting is a fast, simple, and efficient method for simultaneously testing multiple allergens utilizing small amount of antibody...
  66. pmc Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum
    Skyler P Dillon
    Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA
    Biotechnol J 4:1210-4. 2009
    ..We report an average concentration of 113 +/- 40 microg/mL for C1q in non-SLE serum. The assay designed here will be useful in the high-throughput measurement of serum C1q in SLE cases...
  67. ncbi request reprint The genetics of primary Sjögren's syndrome
    Amr H Sawalha
    Arthritis and Immunology Program, Oklahoma Medical Research Foundation, 825 NE 13th Street, Oklahoma City, OK 73104, USA
    Curr Rheumatol Rep 5:324-32. 2003
    ..No doubt a much more comprehensive description of the genetics of this disorder will soon emerge...
  68. doi request reprint Autoantibody prevalence and lupus characteristics in a unique African American population
    Diane L Kamen
    Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA
    Arthritis Rheum 58:1237-47. 2008
    ..Because of the unique homogeneous nature of the Gullah, we sought to define the genetic and environmental factors contributing to systemic lupus erythematosus (SLE) in this population...
  69. ncbi request reprint A nonsense mutation of PEPD in four Amish children with prolidase deficiency
    Heng Wang
    Das Deutsch Center DDC Clinic for Special Needs Children, Middlefield, Ohio 44062, USA
    Am J Med Genet A 140:580-5. 2006
    ..793 T > C in exon 11, resulting in a premature stop-codon at amino acid residue 265 (p.R265X). It is speculated that the severe phenotype in these patients might be associated with the type of the PEPD gene mutation...
  70. ncbi request reprint SOD1 interacts directly with hemoglobin in vitro
    Biji T Kurien
    Thromb Haemost 92:218-20. 2004
  71. ncbi request reprint Anti-Ro and anti-nRNP response in unaffected family members of SLE patients
    Latisha D Heinlen
    Lupus 12:335-7. 2003
  72. ncbi request reprint Milk-alkali syndrome in pre-eclamptic pregnancy: report of a patient and evaluation of albumin-corrected calcium in pre-eclamptic pregnancies
    Stefanie Addington
    J Okla State Med Assoc 99:480-4. 2006
    ..Recommendations for increased oral calcium intake during pregnancy along with frequent use of calcium carbonate for GI symptoms during late pregnancy increase this risk...
  73. ncbi request reprint Four men in a space station - To say nothing of the cow! The quest for finding respite and work in the ultimate frontier
    Biji T Kurien
    Med Hypotheses 68:1195-9. 2007
    ..The anaerobic environment is particularly suitable for biogas generation and if successful they plan to get NASA to launch space vehicles to Mars using this natural fuel with the ISS as the staging area...

Research Grants11

  1. The X chromosome in systemic lupus erythematosus
    R Scofield; Fiscal Year: 2007
    ..This proposal will determine whether the presence of two X chromosomes is a risk factor for women and men with Klinefelter's syndrome (47,XXY). ..
  2. The X chromosome in systemic lupus erythematosus
    R Hal Scofield; Fiscal Year: 2010
    ..This proposal will determine whether the presence of two X chromosomes is a risk factor for women and men with Klinefelter's syndrome (47,XXY). ..
  3. Sjogren's model with anti-Ro/La and Salivary Pathology and Dysfunction
    R Scofield; Fiscal Year: 2007
    ..Improved understanding of the disease at a fundamental level is possible through study of animal models. Such understanding may lead to improved recognition and treatment. ..
  4. Genetics of Severe Systemic Lupus Erythematosus (SLE) defined by thrombocytopenia
    R Scofield; Fiscal Year: 2007
    ..Finally, Specific Aim 4 will assemble a new cohort of families multiplex for SLE so that linkages and associations as well as particular polvmorphisms can be prospectively confirmed. ..
  5. Sjogren's model with anti-Ro/La and Salivary Pathology and Dysfunction
    R Hal Scofield; Fiscal Year: 2010
    ..Improved understanding of the disease at a fundamental level is possible through study of animal models. Such understanding may lead to improved recognition and treatment. ..