Research Topics
Genomes and Genes | Heidi L SchubertSummaryAffiliation: University of Utah Country: USA Publications
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Publications
Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and GColten D Bracken
Department of Physical Science, Southern Utah University, Cedar City, UT 84720 2470, USA
BMC Struct Biol 11:23. 2011..Both isoforms A and G have been structurally characterized in detail, but no structures have been reported for the H isoform which has been found thus far only in members of the halophilic Archaea...- The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelataseHeidi L Schubert
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
EMBO J 21:2068-75. 2002..Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes... - Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenaseHeidi L Schubert
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
Biochem J 415:257-63. 2008.... - Structure and mechanistic implications of a uroporphyrinogen III synthase-product complexHeidi L Schubert
Departments of Biochemistry and Internal Medicine, School of Medicine, University of Utah, Salt Lake City, Utah 84112, USA
Biochemistry 47:8648-55. 2008..A conserved tyrosine residue is potentially positioned to facilitate loss of a hydroxyl from the substrate to initiate the catalytic reaction... - Structure of ATP-bound human ATP:cobalamin adenosyltransferaseHeidi L Schubert
Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112 5650, USA
Biochemistry 45:15188-96. 2006..Ten of the hydrogen bonds are required for structural stability, and four are in positions to interact with cobalamin. The structure also reveals how the point mutations that cause MMA are deficient in these functions... - Many paths to methyltransfer: a chronicle of convergenceHeidi L Schubert
Department of Biochemistry, University of Utah, Salt Lake City 84132 3201, USA
Trends Biochem Sci 28:329-35. 2003..Even within a particular MTase class the amino-acid sequence similarity can be as low as 10%. Thus, the structural and catalytic requirements for methyltransfer from AdoMet appear to be remarkably flexible... 
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferaseHeidi L Schubert
Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132 3201, USA
Biochemistry 42:5592-9. 2003..These structures, therefore, represent intermediates along the catalytic pathway of PrmC and show how the (D/N)PPY motif can be used to select a wide variety substrates...- Structural diversity in metal ion chelation and the structure of uroporphyrinogen III synthaseH L Schubert
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132 3201, USA
Biochem Soc Trans 30:595-600. 2002..The recently derived structure of uroporphyrinogen III synthase reveals a bi-lobed structure in which the active site lies between the domains... - Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunitsAndrew P VanDemark
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
J Biol Chem 283:5058-68. 2008..The docking domain of H2A is identified as an important participant in maintaining stability during yFACT-mediated nucleosome reorganization, suggesting new models for the mechanism of this activity... - Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylationAndrew P VanDemark
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
Mol Cell 27:817-28. 2007..Additional regulatory mechanisms are indicated as H3S10 phosphorylation inhibits Rsc4 binding to H3K14ac peptides... - Biochemical and structural studies of yeast Vps4 oligomerizationMalgorzata D Gonciarz
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
J Mol Biol 384:878-95. 2008..Our mutational analyses demonstrate that pore loop 2 residues Arg241 and Arg251 are required for efficient HIV-1 budding, thereby supporting a role for this "arginine collar" in Vps4 function... - X-ray structures of isopentenyl phosphate kinaseMark F Mabanglo
Department of Chemistry, University of Utah, Salt Lake City, 84112, USA
ACS Chem Biol 5:517-27. 2010..Moreover, His50, Lys5, Lys14, and Lys205 are conserved in all IPKs and can therefore serve as fingerprints for identifying new homologues... - Structural models for interactions between the 20S proteasome and its PAN/19S activatorsBeth M Stadtmueller
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
J Biol Chem 285:13-7. 2010..In the case of the PAN and 19S activators, a penultimate tyrosine/phenylalanine residue contacts the proteasome Gly-19 carbonyl oxygen to stabilize the open conformation... - Structure of an actin-related subcomplex of the SWI/SNF chromatin remodelerHeidi L Schubert
Department of Biochemistry, Department of Oncological Sciences, Huntsman Cancer Institute, and Howard Hughes Medical Institute, University of Utah, Salt Lake City, UT 84112
Proc Natl Acad Sci U S A 110:3345-50. 2013..Thus, our structure provides a foundation for developing models of remodeler function, including mechanisms of coupling between ARPs and the ATPase translocation activity... - Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexesSajjan Koirala
Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
J Cell Biol 191:1127-39. 2010..Our results provide a framework for understanding how adaptors act as scaffolds to orient and stabilize the assembly of dynamins on membranes... - Structural and functional studies on the extracellular domain of BST2/tetherin in reduced and oxidized conformationsHeidi L Schubert
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
Proc Natl Acad Sci U S A 107:17951-6. 2010.... - The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome depositionAndrew P VanDemark
Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
Mol Cell 22:363-74. 2006..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process... - Ubiquitin recognition by the human TSG101 proteinWesley I Sundquist
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
Mol Cell 13:783-9. 2004.... - Structural basis for ESCRT-III protein autoinhibitionMonika Bajorek
Department of Biochemistry, University of Utah, Salt Lake City, Utah, USA
Nat Struct Mol Biol 16:754-62. 2009.... - Potent D-peptide inhibitors of HIV-1 entryBrett D Welch
Department of Biochemistry, University of Utah, Emma Eccles Jones Medical Research Building, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
Proc Natl Acad Sci U S A 104:16828-33. 2007..The D-peptides described here address limitations associated with current L-peptide entry inhibitors and are promising leads for the prevention and treatment of HIV/AIDS... - The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitinFrancisca E Reyes-Turcu
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
Cell 124:1197-208. 2006..These data suggest that binding the ubiquitin C terminus may be necessary for the function of other proteins... - Ubiquitin-binding domainsLinda Hicke
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
Nat Rev Mol Cell Biol 6:610-21. 2005..Such knowledge will be key to our understanding of how ubiquitin regulates cellular proteins and processes... - Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editingMark R Macbeth
Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
Science 309:1534-9. 2005..Indeed, IP6 is also essential for in vivo and in vitro deamination of adenosine 37 of tRNAala by ADAT1... - Structure of a 6-pyruvoyltetrahydropterin synthase homolog from Streptomyces coelicolorJames E Spoonamore
Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:875-9. 2008..However, SCO 6650 maintains active-site residues consistent with binding a pterin-like substrate... - Structure of C73G putidaredoxin from Pseudomonas putidaNatasha Smith
Biotechnology Division of the National Institute of Standards and Technology, Gaithersburg, MD 20899-8312, USA
Acta Crystallogr D Biol Crystallogr 60:816-22. 2004..In addition, the Cys45 amide group donates a hydrogen bond to cluster sulfur S1, with Ala46 adopting an Lalpha conformation, in all three molecules in the crystal... - Crystal structure of the N-terminal domain of the group B streptococcus alpha C proteinThierry C Auperin
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 280:18245-52. 2005..This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level... - Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesisPaul A Davison
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
Biochemistry 44:7603-12. 2005..This mechanism could allow Gun4 to mediate magnesium protoporphyrin levels both for chlorophyll biosynthesis and for signaling to the nucleus... - Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteinsKui Yang
Department of Biochemistry and Molecular Genetics, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045, USA
Proc Natl Acad Sci U S A 105:13817-22. 2008..These results also demonstrate an analogy between a spliceosomal protein and ribosomal proteins that insert extensions into folded rRNAs and stabilize the ribosome... - CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequenceEmma J Petrie
The Protein Crystallography Unit, ARC Centre of Excellence in Structural and Functional Microbial Genomics, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
J Exp Med 205:725-35. 2008..Differences in the docking strategies used by the NKG2D and CD94-NKG2A receptors provided a basis for understanding the promiscuous nature of ligand recognition by NKG2D compared with the fidelity of the CD94-NKG2 receptors... - Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNABranwen M Hall
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
J Mol Biol 375:802-11. 2008..These results highlight the remarkable flexibility of lambda Cro but also suggest that sequence-specific DNA binding may not induce large changes in the protein structure... - Structure of the class IV adenylyl cyclase reveals a novel foldD Travis Gallagher
Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8310, USA
J Mol Biol 362:114-22. 2006..Homologs of Y. pestis AC-IV, including human thiamine triphosphatase, span the three kingdoms of life and delineate an ancient family of phosphonucleotide processing enzymes... - Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutantsLingdi Zhang
Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
Protein Sci 16:1024-31. 2007..Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13... 
Polymerization-defective fibrinogen variant gammaD364A binds knob "A" peptide mimicSheryl R Bowley
Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
Biochemistry 47:8607-13. 2008....