Heidi L Schubert

Summary

Affiliation: University of Utah
Country: USA

Publications

  1. pmc Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and G
    Colten D Bracken
    Department of Physical Science, Southern Utah University, Cedar City, UT 84720 2470, USA
    BMC Struct Biol 11:23. 2011
  2. pmc The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    EMBO J 21:2068-75. 2002
  3. pmc Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
    Biochem J 415:257-63. 2008
  4. pmc Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex
    Heidi L Schubert
    Departments of Biochemistry and Internal Medicine, School of Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 47:8648-55. 2008
  5. pmc Structure of ATP-bound human ATP:cobalamin adenosyltransferase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112 5650, USA
    Biochemistry 45:15188-96. 2006
  6. pmc Many paths to methyltransfer: a chronicle of convergence
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City 84132 3201, USA
    Trends Biochem Sci 28:329-35. 2003
  7. ncbi request reprint Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132 3201, USA
    Biochemistry 42:5592-9. 2003
  8. ncbi request reprint Structural diversity in metal ion chelation and the structure of uroporphyrinogen III synthase
    H L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132 3201, USA
    Biochem Soc Trans 30:595-600. 2002
  9. ncbi request reprint Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    J Biol Chem 283:5058-68. 2008
  10. pmc Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Mol Cell 27:817-28. 2007

Collaborators

Detail Information

Publications33

  1. pmc Crystal structures of a halophilic archaeal malate synthase from Haloferax volcanii and comparisons with isoforms A and G
    Colten D Bracken
    Department of Physical Science, Southern Utah University, Cedar City, UT 84720 2470, USA
    BMC Struct Biol 11:23. 2011
    ..Both isoforms A and G have been structurally characterized in detail, but no structures have been reported for the H isoform which has been found thus far only in members of the halophilic Archaea...
  2. pmc The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    EMBO J 21:2068-75. 2002
    ..Analysis of mutant proteins suggests that both catalytic activities share a single active site, and that Asp141 plays an essential role in both dehydrogenase and chelatase processes...
  3. pmc Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
    Biochem J 415:257-63. 2008
    ....
  4. pmc Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex
    Heidi L Schubert
    Departments of Biochemistry and Internal Medicine, School of Medicine, University of Utah, Salt Lake City, Utah 84112, USA
    Biochemistry 47:8648-55. 2008
    ..A conserved tyrosine residue is potentially positioned to facilitate loss of a hydroxyl from the substrate to initiate the catalytic reaction...
  5. pmc Structure of ATP-bound human ATP:cobalamin adenosyltransferase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84112 5650, USA
    Biochemistry 45:15188-96. 2006
    ..Ten of the hydrogen bonds are required for structural stability, and four are in positions to interact with cobalamin. The structure also reveals how the point mutations that cause MMA are deficient in these functions...
  6. pmc Many paths to methyltransfer: a chronicle of convergence
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City 84132 3201, USA
    Trends Biochem Sci 28:329-35. 2003
    ..Even within a particular MTase class the amino-acid sequence similarity can be as low as 10%. Thus, the structural and catalytic requirements for methyltransfer from AdoMet appear to be remarkably flexible...
  7. ncbi request reprint Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, Utah 84132 3201, USA
    Biochemistry 42:5592-9. 2003
    ..These structures, therefore, represent intermediates along the catalytic pathway of PrmC and show how the (D/N)PPY motif can be used to select a wide variety substrates...
  8. ncbi request reprint Structural diversity in metal ion chelation and the structure of uroporphyrinogen III synthase
    H L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132 3201, USA
    Biochem Soc Trans 30:595-600. 2002
    ..The recently derived structure of uroporphyrinogen III synthase reveals a bi-lobed structure in which the active site lies between the domains...
  9. ncbi request reprint Structural and functional analysis of the Spt16p N-terminal domain reveals overlapping roles of yFACT subunits
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    J Biol Chem 283:5058-68. 2008
    ..The docking domain of H2A is identified as an important participant in maintaining stability during yFACT-mediated nucleosome reorganization, suggesting new models for the mechanism of this activity...
  10. pmc Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA
    Mol Cell 27:817-28. 2007
    ..Additional regulatory mechanisms are indicated as H3S10 phosphorylation inhibits Rsc4 binding to H3K14ac peptides...
  11. pmc Biochemical and structural studies of yeast Vps4 oligomerization
    Malgorzata D Gonciarz
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112 5650, USA
    J Mol Biol 384:878-95. 2008
    ..Our mutational analyses demonstrate that pore loop 2 residues Arg241 and Arg251 are required for efficient HIV-1 budding, thereby supporting a role for this "arginine collar" in Vps4 function...
  12. pmc X-ray structures of isopentenyl phosphate kinase
    Mark F Mabanglo
    Department of Chemistry, University of Utah, Salt Lake City, 84112, USA
    ACS Chem Biol 5:517-27. 2010
    ..Moreover, His50, Lys5, Lys14, and Lys205 are conserved in all IPKs and can therefore serve as fingerprints for identifying new homologues...
  13. pmc Structural models for interactions between the 20S proteasome and its PAN/19S activators
    Beth M Stadtmueller
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, Utah 84112, USA
    J Biol Chem 285:13-7. 2010
    ..In the case of the PAN and 19S activators, a penultimate tyrosine/phenylalanine residue contacts the proteasome Gly-19 carbonyl oxygen to stabilize the open conformation...
  14. pmc Structure of an actin-related subcomplex of the SWI/SNF chromatin remodeler
    Heidi L Schubert
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
    Proc Natl Acad Sci U S A 110:3345-50. 2013
    ..Thus, our structure provides a foundation for developing models of remodeler function, including mechanisms of coupling between ARPs and the ATPase translocation activity...
  15. pmc Molecular architecture of a dynamin adaptor: implications for assembly of mitochondrial fission complexes
    Sajjan Koirala
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84112, USA
    J Cell Biol 191:1127-39. 2010
    ..Our results provide a framework for understanding how adaptors act as scaffolds to orient and stabilize the assembly of dynamins on membranes...
  16. pmc Structural and functional studies on the extracellular domain of BST2/tetherin in reduced and oxidized conformations
    Heidi L Schubert
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 107:17951-6. 2010
    ....
  17. ncbi request reprint The structure of the yFACT Pob3-M domain, its interaction with the DNA replication factor RPA, and a potential role in nucleosome deposition
    Andrew P VanDemark
    Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, 84132, USA
    Mol Cell 22:363-74. 2006
    ..These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process...
  18. ncbi request reprint Ubiquitin recognition by the human TSG101 protein
    Wesley I Sundquist
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Mol Cell 13:783-9. 2004
    ....
  19. pmc Structural basis for ESCRT-III protein autoinhibition
    Monika Bajorek
    Department of Biochemistry, University of Utah, Salt Lake City, Utah, USA
    Nat Struct Mol Biol 16:754-62. 2009
    ....
  20. pmc Potent D-peptide inhibitors of HIV-1 entry
    Brett D Welch
    Department of Biochemistry, University of Utah, Emma Eccles Jones Medical Research Building, 15 North Medical Drive East, Salt Lake City, UT 84112 5650, USA
    Proc Natl Acad Sci U S A 104:16828-33. 2007
    ..The D-peptides described here address limitations associated with current L-peptide entry inhibitors and are promising leads for the prevention and treatment of HIV/AIDS...
  21. ncbi request reprint The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin
    Francisca E Reyes-Turcu
    Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA
    Cell 124:1197-208. 2006
    ..These data suggest that binding the ubiquitin C terminus may be necessary for the function of other proteins...
  22. ncbi request reprint Ubiquitin-binding domains
    Linda Hicke
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, Illinois 60208 3500, USA
    Nat Rev Mol Cell Biol 6:610-21. 2005
    ..Such knowledge will be key to our understanding of how ubiquitin regulates cellular proteins and processes...
  23. pmc Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing
    Mark R Macbeth
    Department of Biochemistry, University of Utah, Salt Lake City, UT 84132, USA
    Science 309:1534-9. 2005
    ..Indeed, IP6 is also essential for in vivo and in vitro deamination of adenosine 37 of tRNAala by ADAT1...
  24. pmc Structure of a 6-pyruvoyltetrahydropterin synthase homolog from Streptomyces coelicolor
    James E Spoonamore
    Department of Biochemistry, University of Arizona, Tucson, AZ 85721, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:875-9. 2008
    ..However, SCO 6650 maintains active-site residues consistent with binding a pterin-like substrate...
  25. ncbi request reprint Structure of C73G putidaredoxin from Pseudomonas putida
    Natasha Smith
    Biotechnology Division of the National Institute of Standards and Technology, Gaithersburg, MD 20899 8312, USA
    Acta Crystallogr D Biol Crystallogr 60:816-22. 2004
    ..In addition, the Cys45 amide group donates a hydrogen bond to cluster sulfur S1, with Ala46 adopting an Lalpha conformation, in all three molecules in the crystal...
  26. ncbi request reprint Crystal structure of the N-terminal domain of the group B streptococcus alpha C protein
    Thierry C Auperin
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:18245-52. 2005
    ..This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level...
  27. ncbi request reprint Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis
    Paul A Davison
    Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK
    Biochemistry 44:7603-12. 2005
    ..This mechanism could allow Gun4 to mediate magnesium protoporphyrin levels both for chlorophyll biosynthesis and for signaling to the nucleus...
  28. pmc Crystal structure of the beta-finger domain of Prp8 reveals analogy to ribosomal proteins
    Kui Yang
    Department of Biochemistry and Molecular Genetics, Anschutz Medical Campus, University of Colorado Denver, Aurora, CO 80045, USA
    Proc Natl Acad Sci U S A 105:13817-22. 2008
    ..These results also demonstrate an analogy between a spliceosomal protein and ribosomal proteins that insert extensions into folded rRNAs and stabilize the ribosome...
  29. pmc CD94-NKG2A recognition of human leukocyte antigen (HLA)-E bound to an HLA class I leader sequence
    Emma J Petrie
    The Protein Crystallography Unit, ARC Centre of Excellence in Structural and Functional Microbial Genomics, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
    J Exp Med 205:725-35. 2008
    ..Differences in the docking strategies used by the NKG2D and CD94-NKG2A receptors provided a basis for understanding the promiscuous nature of ligand recognition by NKG2D compared with the fidelity of the CD94-NKG2 receptors...
  30. ncbi request reprint Two structures of a lambda Cro variant highlight dimer flexibility but disfavor major dimer distortions upon specific binding of cognate DNA
    Branwen M Hall
    Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
    J Mol Biol 375:802-11. 2008
    ..These results highlight the remarkable flexibility of lambda Cro but also suggest that sequence-specific DNA binding may not induce large changes in the protein structure...
  31. ncbi request reprint Structure of the class IV adenylyl cyclase reveals a novel fold
    D Travis Gallagher
    Biochemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 8310, USA
    J Mol Biol 362:114-22. 2006
    ..Homologs of Y. pestis AC-IV, including human thiamine triphosphatase, span the three kingdoms of life and delineate an ancient family of phosphonucleotide processing enzymes...
  32. pmc Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants
    Lingdi Zhang
    Department of Biochemistry and Molecular Genetics, University of Colorado at Denver and Health Sciences Center, Aurora, Colorado 80045, USA
    Protein Sci 16:1024-31. 2007
    ..Mapping of RP13 mutants on the Prp8 structure suggests that these residues constitute a binding surface between Prp8 and other partner(s), and the disruption of this interaction provides a plausible molecular mechanism for RP13...
  33. doi request reprint Polymerization-defective fibrinogen variant gammaD364A binds knob "A" peptide mimic
    Sheryl R Bowley
    Department of Chemistry, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:8607-13. 2008
    ....