Olaf Schneewind

Summary

Affiliation: University of Chicago
Country: USA

Publications

  1. pmc Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria
    Olaf Schneewind
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA Electronic address
    Biochim Biophys Acta 1843:1687-97. 2014
  2. pmc Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood
    Molly McAdow
    Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America
    PLoS Pathog 7:e1002307. 2011
  3. pmc Vaccine protection against Staphylococcus aureus pneumonia
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Exp Med 205:287-94. 2008
  4. pmc Contribution of coagulases towards Staphylococcus aureus disease and protective immunity
    Alice G Cheng
    Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America
    PLoS Pathog 6:e1001036. 2010
  5. pmc Role of protein A in the evasion of host adaptive immune responses by Staphylococcus aureus
    Fabiana Falugi
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    MBio 4:e00575-13. 2013
  6. pmc Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus
    Chen Chen
    From the Department of Microbiology, University of Chicago, Chicago, Illinois 60637
    J Biol Chem 288:29440-52. 2013
  7. pmc Protein secretion and surface display in Gram-positive bacteria
    Olaf Schneewind
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Philos Trans R Soc Lond B Biol Sci 367:1123-39. 2012
  8. pmc Yersinia pestis IS1541 transposition provides for escape from plague immunity
    Claire A Cornelius
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Infect Immun 77:1807-16. 2009
  9. ncbi request reprint Iron-source preference of Staphylococcus aureus infections
    Eric P Skaar
    Committee on Microbiology, 920 East 58th Street, University of Chicago, Chicago, IL 60637, USA
    Science 305:1626-8. 2004
  10. pmc Cell wall anchor structure of BcpA pili in Bacillus anthracis
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 283:36676-86. 2008

Research Grants

Detail Information

Publications100

  1. pmc Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria
    Olaf Schneewind
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA Electronic address
    Biochim Biophys Acta 1843:1687-97. 2014
    ..This article is part of a Special Issue entitled: Protein trafficking and secretion in bacteria. Guest Editors: Anastassios Economou and Ross Dalbey. ..
  2. pmc Preventing Staphylococcus aureus sepsis through the inhibition of its agglutination in blood
    Molly McAdow
    Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America
    PLoS Pathog 7:e1002307. 2011
    ..Together these results suggest that the combined administration of direct thrombin inhibitors and ClfA-antibodies that block S. aureus agglutination with fibrin may be useful for the prevention of staphylococcal sepsis in humans...
  3. pmc Vaccine protection against Staphylococcus aureus pneumonia
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Exp Med 205:287-94. 2008
    ..aureus challenge and prevents the injury of human lung epithelial cells during infection. Thus, Hla vaccination or immunotherapy may prevent S. aureus pneumonia in humans...
  4. pmc Contribution of coagulases towards Staphylococcus aureus disease and protective immunity
    Alice G Cheng
    Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America
    PLoS Pathog 6:e1001036. 2010
    ..Coa and vWbp specific antibodies confer protection against abscess formation and S. aureus lethal bacteremia, suggesting that coagulases function as protective antigens for a staphylococcal vaccine...
  5. pmc Role of protein A in the evasion of host adaptive immune responses by Staphylococcus aureus
    Fabiana Falugi
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    MBio 4:e00575-13. 2013
    ..aureus SpA were abolished in µMT mice lacking mature B cells and antibodies. Thus, while wild-type S. aureus escapes host immune surveillance, the spaKKAA variant elicits adaptive responses that protect against recurrent infection...
  6. pmc Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus
    Chen Chen
    From the Department of Microbiology, University of Chicago, Chicago, Illinois 60637
    J Biol Chem 288:29440-52. 2013
    ..Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis. ..
  7. pmc Protein secretion and surface display in Gram-positive bacteria
    Olaf Schneewind
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Philos Trans R Soc Lond B Biol Sci 367:1123-39. 2012
    ..Gram-positive bacteria also secrete WXG100 proteins and carry unique genes that either contribute to discrete steps in secretion or represent distinctive substrates for protein transport reactions...
  8. pmc Yersinia pestis IS1541 transposition provides for escape from plague immunity
    Claire A Cornelius
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Infect Immun 77:1807-16. 2009
    ..These findings suggest a model in which IS1541 insertion into caf1A provides for reversible changes in envelope structure, enabling Y. pestis to escape from adaptive immune responses and plague immunity...
  9. ncbi request reprint Iron-source preference of Staphylococcus aureus infections
    Eric P Skaar
    Committee on Microbiology, 920 East 58th Street, University of Chicago, Chicago, IL 60637, USA
    Science 305:1626-8. 2004
    ..aureus. Heme iron scavenging by means of hts is required for staphylococcal pathogenesis in animal hosts, indicating that heme iron is the preferred iron source during the initiation of infection...
  10. pmc Cell wall anchor structure of BcpA pili in Bacillus anthracis
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 283:36676-86. 2008
    ..These results represent the first demonstration of a cell wall anchor structure for pili, which are deposited by sortase A into the envelope of many different microbes...
  11. pmc Yersinia pestis caf1 variants and the limits of plague vaccine protection
    Lauriane E Quenee
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    Infect Immun 76:2025-36. 2008
    ....
  12. pmc IsdA and IsdB antibodies protect mice against Staphylococcus aureus abscess formation and lethal challenge
    Hwan Keun Kim
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL, USA
    Vaccine 28:6382-92. 2010
    ....
  13. pmc Immunization with recombinant V10 protects cynomolgus macaques from lethal pneumonic plague
    Claire A Cornelius
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    Infect Immun 76:5588-97. 2008
    ....
  14. pmc Staphylococcus aureus synthesizes adenosine to escape host immune responses
    Vilasack Thammavongsa
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Exp Med 206:2417-27. 2009
    ..Collectively, these results suggest that staphylococci and other bacterial pathogens exploit the immunomodulatory attributes of adenosine to escape host immune responses...
  15. pmc Sortase D forms the covalent bond that links BcpB to the tip of Bacillus cereus pili
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Biol Chem 284:12989-97. 2009
    ..Thus, unique attributes of the sorting signals of minor pilins provide Gram-positive bacteria with a universal mechanism ordering assembly of pili...
  16. pmc Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope
    Andrew H Gaspar
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Bacteriol 187:4646-55. 2005
    ..These results suggest that B. anthracis SrtA anchors surface proteins bearing LPXTG motif sorting signals to the cell wall envelope of vegetative bacilli...
  17. pmc Immunogenicity and protective immunity against bubonic plague and pneumonic plague by immunization of mice with the recombinant V10 antigen, a variant of LcrV
    Kristin L Debord
    Department of Microbiology, University of Chicago, 920 E 58th Street, Chicago, Illinois 60637, USA
    Infect Immun 74:4910-4. 2006
    ..Immunization with rV10 generates robust antibody responses that protect mice against bubonic plague and pneumonic plague, suggesting that rV10 may serve as an improved plague vaccine...
  18. pmc Signal peptides direct surface proteins to two distinct envelope locations of Staphylococcus aureus
    Andrea DeDent
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    EMBO J 27:2656-68. 2008
    ....
  19. pmc Hereditary hemochromatosis restores the virulence of plague vaccine strains
    Lauriane E Quenee
    Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, University of Chicago, Illinois 60637, USA
    J Infect Dis 206:1050-8. 2012
    ..pestis type III secretion generated protection against plague. Thus, individuals with hereditary hemochromatosis may be protected with subunit vaccines but should not be exposed to live-attenuated plague vaccines...
  20. pmc A synonymous mutation in Yersinia enterocolitica yopE affects the function of the YopE type III secretion signal
    Kumaran S Ramamurthi
    Department of Microbiology, University of Chicago, 920 East 58th St, Chicago, IL 60637, USA
    J Bacteriol 187:707-15. 2005
    ..Thus, yopE mRNA appears to encode a property that mediates the type III injection of YopE...
  21. pmc Rejection of impassable substrates by Yersinia type III secretion machines
    Joseph A Sorg
    Department of Microbiology, University of Chicago, Illinois 60637, USA
    J Bacteriol 187:7090-102. 2005
    ..Genetic suppression of the type III blockade does not, however, promote YopE-DHFR secretion. A model is proposed whereby rejection of YopE-DHFR from the secretion pathway inhibits type III gene expression...
  22. pmc Plague in Guinea pigs and its prevention by subunit vaccines
    Lauriane E Quenee
    Department of Microbiology, The University of Chicago, Chicago, Illinois Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, IL 60637, USA
    Am J Pathol 178:1689-700. 2011
    ..pestis CO92, immunization with recombinant F1 did not. rV10, a vaccine variant lacking LcrV residues 271-300, elicited protection against pneumonic plague, which seemed to be based on conformational antibodies directed against LcrV...
  23. pmc Intramolecular amide bonds stabilize pili on the surface of bacilli
    Jonathan M Budzik
    Departments of Microbiology and Chemistry, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 106:19992-7. 2009
    ..We report that the domains of pilin precursors have evolved to synthesize a discrete sequence of intramolecular amide bonds, thereby conferring structural stability and protease resistance to pili...
  24. pmc Polymorphisms in the lcrV gene of Yersinia enterocolitica and their effect on plague protective immunity
    Nathan C Miller
    Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Argonne, Illinois, USA
    Infect Immun 80:1572-82. 2012
    ..enterocolitica strains, expression of lcrV(W22703) or lcrV(WA-314) in Y. pestis did not allow these strains to escape LcrV-mediated plague protective immunity in the intravenous challenge model...
  25. ncbi request reprint Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring
    Adrienne M Perry
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 277:16241-8. 2002
    ....
  26. pmc Vaccine assembly from surface proteins of Staphylococcus aureus
    Yukiko K Stranger-Jones
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 103:16942-7. 2006
    ..When assembled into a combined vaccine, the four surface proteins afforded high levels of protection against invasive disease or lethal challenge with human clinical S. aureus isolates...
  27. pmc Amino acid residues 196-225 of LcrV represent a plague protective epitope
    Lauriane E Quenee
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Vaccine 28:1870-6. 2010
    ..These results identify LcrV residues 196-225 as a linear epitope that is recognized by the murine immune system to confer plague protection...
  28. pmc Amide bonds assemble pili on the surface of bacilli
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 105:10215-20. 2008
    ..Conservation of catalysts and structural elements in pilin precursors in Gram-positive bacteria suggests a universal mechanism of fiber assembly...
  29. doi request reprint Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis
    Taeok Bae
    Department of Microbiology, The University of Chicago, Chicago, IL, USA
    Methods Mol Biol 416:103-16. 2008
    ..aureus. This chapter describes a procedure for isolating mutants and mapping the insertion sites on the chromosome...
  30. pmc Activation of inhibitors by sortase triggers irreversible modification of the active site
    Anthony W Maresso
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 282:23129-39. 2007
    ..Analysis of the three-dimensional structure of Bacillus anthracis sortase B with and without inhibitor provides insights into the mechanism of inhibition and reveals binding pockets that can be exploited for drug discovery...
  31. pmc Plague bacteria target immune cells during infection
    Melanie M Marketon
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Science 309:1739-41. 2005
    ..Thus, it appears that Y. pestis disables these cell populations to annihilate host immune responses during plague...
  32. pmc Nontoxigenic protein A vaccine for methicillin-resistant Staphylococcus aureus infections in mice
    Hwan Keun Kim
    Department of Microbiology, University of Chicago, Chicago, IL 60637
    J Exp Med 207:1863-70. 2010
    ..Furthermore, SpA(KKAA) immunization enabled MRSA-challenged mice to mount antibody responses to many different staphylococcal antigens...
  33. pmc Genetic requirements for Staphylococcus aureus abscess formation and persistence in host tissues
    Alice G Cheng
    Department of Microbiology, University of Chicago, 920 East 58th St, Chicago, IL 60637, USA
    FASEB J 23:3393-404. 2009
    ..Thus, staphylococci employ envelope proteins at discrete stages of a developmental program that enables abscess formation and bacterial persistence in host tissues...
  34. pmc N-Acetylglucosaminylation of Serine-Aspartate Repeat Proteins Promotes Staphylococcus aureus Bloodstream Infection
    Lena Thomer
    From the Department of Microbiology, The University of Chicago, Chicago, Illinois 60637 and
    J Biol Chem 289:3478-86. 2014
    ..However, only sdgB is essential for GlcNAc modification, and an sdgB mutant is defective in the pathogenesis of sepsis in mice. Thus, GlcNAc modification of proteins promotes S. aureus replication in the bloodstream of mammalian hosts. ..
  35. pmc Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria
    Luciano A Marraffini
    Department of Microbiology, Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Microbiol Mol Biol Rev 70:192-221. 2006
    ..This review focuses on the mechanisms of surface protein anchoring to the cell wall envelope by sortases and the role that these enzymes play in bacterial physiology and pathogenesis...
  36. pmc Coagulases as determinants of protective immune responses against Staphylococcus aureus
    Molly McAdow
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    Infect Immun 80:3389-98. 2012
    ..aureus strains in mice was derived...
  37. pmc An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis
    Sarkis K Mazmanian
    Committee on Microbiology, Department of Molecular Genetics and Cell Biology, University of Chicago, 920 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 99:2293-8. 2002
    ..Cell wall-anchored surface proteins and the isd locus seem involved in a novel mechanism of iron acquisition that is important for bacterial pathogenesis...
  38. pmc Secretion signal recognition by YscN, the Yersinia type III secretion ATPase
    Joseph A Sorg
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 103:16490-5. 2006
    ....
  39. pmc Two capsular polysaccharides enable Bacillus cereus G9241 to cause anthrax-like disease
    So Young Oh
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 80:455-70. 2011
    ..Thus, multiple different gene clusters endow pathogenic bacilli with capsular material, provide for escape from innate host immune responses and aid in establishing the pathogenesis of anthrax-like disease...
  40. pmc Capsule anchoring in Bacillus anthracis occurs by a transpeptidation reaction that is inhibited by capsidin
    Stefan Richter
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 71:404-20. 2009
    ..Capsidin treatment blocked capsular assembly by B. anthracis and enabled phagocytic killing of non-encapsulated vegetative forms...
  41. pmc Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus
    ANGELIKA GRUNDLING
    Department of Microbiology, 920 East 58th Street, Chicago, IL 60637, USA
    J Bacteriol 189:2521-30. 2007
    ..Glycolipid anchoring of LTA appears to play an important role during infection, as S. aureus variants lacking ltaA display defects in the pathogenesis of animal infections...
  42. pmc Yersinia enterocolitica type III secretion of YopR requires a structure in its mRNA
    Bill Blaylock
    Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 70:1210-22. 2008
    ..Silent mutations in the second signal abrogate function and the phenotype of other mutations can be suppressed by secondary mutations predicted to restore base complementary in a 3' stem-loop structure of the yopR mRNA...
  43. pmc YfbA, a Yersinia pestis regulator required for colonization and biofilm formation in the gut of cat fleas
    Christina Tam
    Howard Taylor Ricketts Laboratory, Argonne National Laboratory, Lemont, Illinois, USA Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    J Bacteriol 196:1165-73. 2014
    ..Examining a library of transposon insertion mutants, we identified the LysR-type transcriptional regulator YfbA, which is essential for Y. pestis colonization and biofilm formation in cat fleas. ..
  44. ncbi request reprint Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A
    Luciano A Marraffini
    Committee on Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Biol Chem 279:37763-70. 2004
    ....
  45. ncbi request reprint Allelic replacement in Staphylococcus aureus with inducible counter-selection
    Taeok Bae
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Plasmid 55:58-63. 2006
    ..Using this strategy, allelic replacements in S. aureus rocA were generated at frequencies that obviated the need for antibiotic marker selection...
  46. ncbi request reprint Passage of heme-iron across the envelope of Staphylococcus aureus
    Sarkis K Mazmanian
    Committee on Microbiology, Department of Molecular Genetics and Cell Biology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Science 299:906-9. 2003
    ..Thus, Isd appears to act as an import apparatus that uses cell wall-anchored proteins to relay heme-iron across the bacterial envelope...
  47. pmc Recurrent infections and immune evasion strategies of Staphylococcus aureus
    Hwan Keun Kim
    Department of Microbiology, University of Chicago, Chicago, IL 60637, United States
    Curr Opin Microbiol 15:92-9. 2012
    ..We discuss strategies how the three secreted products of staphylococci may be exploited for the development of vaccines and therapeutics...
  48. ncbi request reprint Protein sorting to the cell wall envelope of Gram-positive bacteria
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Biochim Biophys Acta 1694:269-78. 2004
    ....
  49. pmc A play in four acts: Staphylococcus aureus abscess formation
    Alice G Cheng
    Department of Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    Trends Microbiol 19:225-32. 2011
    ..Protein A and coagulases are distinctive virulence attributes for S. aureus, and humoral immune responses specific for these polypeptides provide protection against abscess formation in animal models of staphylococcal disease...
  50. pmc Surface protein IsdC and Sortase B are required for heme-iron scavenging of Bacillus anthracis
    Anthony W Maresso
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Bacteriol 188:8145-52. 2006
    ..B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme...
  51. pmc ABI domain-containing proteins contribute to surface protein display and cell division in Staphylococcus aureus
    Matthew B Frankel
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 78:238-52. 2010
    ....
  52. pmc Toll-like receptor 6 drives differentiation of tolerogenic dendritic cells and contributes to LcrV-mediated plague pathogenesis
    R William DePaolo
    Department of Medicine, University of Chicago, Chicago, IL 60637, USA
    Cell Host Microbe 4:350-61. 2008
    ..These results explain why TLR2 can mediate both pro- and anti-inflammatory responses and identify TLR6 as a distinct receptor driving regulatory IL-10 responses...
  53. pmc YopR impacts type III needle polymerization in Yersinia species
    Bill Blaylock
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 75:221-9. 2010
    ..An alternative possibility, which YopR participates directly in the polymerization of YscF, seems less likely as YopR is not associated with purified needles...
  54. pmc Impassable YscP substrates and their impact on the Yersinia enterocolitica type III secretion pathway
    Kelly E Riordan
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    J Bacteriol 190:6204-16. 2008
    ..YscP-GST copurified with the type III ATPase complex (YscN, YscL, and YscQ) and with YscO, suggesting that the association of specific machine components with the impassable substrate may cause the block in type III secretion...
  55. pmc Distribution of protein A on the surface of Staphylococcus aureus
    Andrea C Dedent
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Bacteriol 189:4473-84. 2007
    ..A model is presented whereby protein A deposition at discrete sites coupled with cell wall synthesis enables distribution of protein A on the staphylococcal surface...
  56. pmc Multiple ligands of von Willebrand factor-binding protein (vWbp) promote Staphylococcus aureus clot formation in human plasma
    Lena Thomer
    From the Department of Microbiology, University of Chicago, Chicago, Illinois 60637
    J Biol Chem 288:28283-92. 2013
    ..FXIII activity in staphylococcal clots could be attributed to thrombin-dependent proteolytic activation as well as vWbp-mediated non-proteolytic activation of FXIII zymogen. ..
  57. pmc Staphylococcus aureus mutants lacking the LytR-CpsA-Psr family of enzymes release cell wall teichoic acids into the extracellular medium
    Yvonne G Y Chan
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    J Bacteriol 195:4650-9. 2013
    ..We propose a model whereby the S. aureus Δlcp mutant, defective in tethering WTA to the cell wall, cleaves WTA synthesis intermediates, releasing ribitol phosphate into the medium and recycling bactoprenol for peptidoglycan synthesis. ..
  58. pmc The secretion signal of YopN, a regulatory protein of the Yersinia enterocolitica type III secretion pathway
    John W Goss
    Committee on Microbiology, The University of Chicago, 920 E 58th St, Chicago, IL 60637, USA
    J Bacteriol 186:6320-4. 2004
    ..yopN codons 1 to 12 were identified as critical for signal function. Several synonymous mutations that abolish secretion of hybrid proteins without altering the codon specificity of yopN mRNA were identified...
  59. ncbi request reprint Ubiquitin-Yop hybrids as probes for post-translational transport by the Yersinia type III secretion pathway
    Lauriane E Quenee
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 65:386-400. 2007
    ..These results suggest that recognition and secretion of Yop substrates by the type III machinery can occur by a post-translational mechanism...
  60. ncbi request reprint Assembly of pili in Gram-positive bacteria
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Trends Microbiol 12:228-34. 2004
  61. pmc The ttsA gene is required for low-calcium-induced type III secretion of Yop proteins and virulence of Yersinia enterocolitica W22703
    Kristin L Debord
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Bacteriol 185:3499-507. 2003
    ..The ttsA insertion caused a greater-than-3-log-unit reduction in virulence compared to that of the parental strain...
  62. ncbi request reprint The Bacillus subtilis quorum-sensing molecule CSF contributes to intestinal homeostasis via OCTN2, a host cell membrane transporter
    Mikihiro Fujiya
    Martin Boyer Laboratories, University of Chicago, Chicago, IL 60637, USA
    Cell Host Microbe 1:299-308. 2007
    ..Thus, OCTN2-mediated CSF transport serves as an example of a host-bacterial interaction that allows the host to monitor and respond to changes in the behavior or composition of colonic flora...
  63. ncbi request reprint Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Mol Microbiol 53:251-61. 2004
    ..Thus, pilus assembly in Gram-positive bacteria seems to occur by a universal mechanism of ordered cross-linking of precursor proteins, the multiple conserved features of which are recognized by designated sortase enzymes...
  64. pmc Sortase C-mediated anchoring of BasI to the cell wall envelope of Bacillus anthracis
    Luciano A Marraffini
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Bacteriol 189:6425-36. 2007
    ..anthracis peptidoglycan...
  65. doi request reprint Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds
    Antoni P A Hendrickx
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, Illinois 60637, USA
    Nat Rev Microbiol 9:166-76. 2011
    ..Together, these isopeptide bonds endow these sortase products with adhesive properties and resistance to host proteases...
  66. pmc Prevention of pneumonic plague in mice, rats, guinea pigs and non-human primates with clinical grade rV10, rV10-2 or F1-V vaccines
    Lauriane E Quenee
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Vaccine 29:6572-83. 2011
    ..The data support further development of rV10-2 for FDA Investigational New Drug (IND) authorization review and clinical testing...
  67. ncbi request reprint Plague vaccines and the molecular basis of immunity against Yersinia pestis
    Lauriane E Quenee
    Department of Microbiology, University of Chicago, Chicago, IL, USA
    Hum Vaccin 5:817-23. 2009
    ..Several subunit vaccines, for example rF1+rV (rYP002), rF1V or rV10, are being developed to generate plague protection in humans. Efficacy testing and licensure for human use requires the establishment of correlates for plague immunity...
  68. ncbi request reprint Substrate recognition of type III secretion machines--testing the RNA signal hypothesis
    Joseph A Sorg
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Cell Microbiol 7:1217-25. 2005
    ..This review summarizes experimental observations and mechanistic models for substrate recognition in this field...
  69. ncbi request reprint IsdG and IsdI, heme-degrading enzymes in the cytoplasm of Staphylococcus aureus
    Eric P Skaar
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 279:436-43. 2004
    ..Thus, it appears that bacterial pathogens evolved different strategies to retrieve iron from scavenged heme molecules and that staphylococcal IsdG and IsdI represent examples of bacterial heme-oxygenases...
  70. ncbi request reprint Yersinia yopQ mRNA encodes a bipartite type III secretion signal in the first 15 codons
    Kumaran S Ramamurthi
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    Mol Microbiol 50:1189-98. 2003
    ..Thus, elements downstream of the minimal secretion signal of YopQ increase the efficiency of YopQ secretion and suppress mutations elsewhere in the secretion signal...
  71. pmc Binding of SycH chaperone to YscM1 and YscM2 activates effector yop expression in Yersinia enterocolitica
    Eric D Cambronne
    Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA
    J Bacteriol 186:829-41. 2004
    ..Taken together, these results suggest that YscM1- and YscM2-mediated repression may be relieved through binding to the cytoplasmic chaperone SycH prior to their type III injection into host cells...
  72. pmc Characterization of the Yersinia enterocolitica type III secretion ATPase YscN and its regulator, YscL
    Bill Blaylock
    Department of Microbiology, University of Chicago, IL 60627, USA
    J Bacteriol 188:3525-34. 2006
    ..In vivo, YscL and YscN interact with each other, and the overexpression of glutathione S-transferase-YscL abolishes secretion and down-regulates the expression of secretion apparatus components...
  73. pmc YscU cleavage and the assembly of Yersinia type III secretion machine complexes
    Kelly E Riordan
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 68:1485-501. 2008
    ..A model is proposed whereby YscU auto-cleavage promotes interaction with YscL and recruitment of ATPase complexes that initiate type III secretion...
  74. pmc Enzymatic properties of Staphylococcus aureus adenosine synthase (AdsA)
    Vilasack Thammavongsa
    Department of Microbiology, University of Chicago, 920 E, 58th St, Chicago, Illinois 60637, USA
    BMC Biochem 12:56. 2011
    ..NTPDases harbor apyrase conserved regions (ACRs) that are critical for activity...
  75. ncbi request reprint Assembly of pili on the surface of Bacillus cereus vegetative cells
    Jonathan M Budzik
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 66:495-510. 2007
    ..B. anthracis sortase A (srtA), but not sortase B (srtB) or C (srtC), is required for proper anchoring of pili to the bacterial envelope, suggesting that BcpA/BcpB pili are linked to peptidoglycan cross-bridges...
  76. ncbi request reprint Prophages of Staphylococcus aureus Newman and their contribution to virulence
    Taeok Bae
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 62:1035-47. 2006
    ..S. aureus Newman lacking all four prophages was unable to cause disease, thereby revealing essential contributions of prophages to the pathogenesis of staphylococcal infections...
  77. pmc Identifying protective antigens of Staphylococcus aureus, a pathogen that suppresses host immune responses
    Hwan Keun Kim
    Department of Microbiology, University of Chicago, 920 East 58 Street, Chicago, IL 60637, USA
    FASEB J 25:3605-12. 2011
    ..Genetic vaccinology is particularly useful for microbes that do not elicit natural protective immunity during infection...
  78. pmc Panton-Valentine leukocidin is not a virulence determinant in murine models of community-associated methicillin-resistant Staphylococcus aureus disease
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, Chicago, Illinois, USA
    J Infect Dis 198:1166-70. 2008
    ..The data presented in this report support the observation that PVL does not contribute to the pathogenesis of staphylococcal infection of mice...
  79. pmc BslA, the S-layer adhesin of B. anthracis, is a virulence factor for anthrax pathogenesis
    Justin Kern
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Mol Microbiol 75:324-32. 2010
    ..Together these results suggest that BslA functions as the surface adhesin of the anthrax pathogen B. anthracis strain Ames...
  80. pmc Bacillus anthracis secretes proteins that mediate heme acquisition from hemoglobin
    Anthony W Maresso
    Department of Microbiology, University of Chicago, Chicago, Illinois, United States of America
    PLoS Pathog 4:e1000132. 2008
    ..anthracis seems to have also evolved NEAT domain proteins in the extracellular milieu and in the bacterial envelope to provide for the passage of heme...
  81. doi request reprint BslA, a pXO1-encoded adhesin of Bacillus anthracis
    Justin W Kern
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL, USA
    Mol Microbiol 68:504-15. 2008
    ..Twenty-three B. anthracis genes, whose products harbour similar SLH domains, may provide additional surface molecules that allow bacilli to engage cells or tissues of specific hosts during anthrax pathogenesis...
  82. pmc Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus
    ANGELIKA GRUNDLING
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 104:8478-83. 2007
    ..Thus, LtaS inhibition can be used as a target to treat human infections caused by antibiotic-resistant S. aureus or other bacterial pathogens...
  83. ncbi request reprint Bacillus anthracis surface-layer proteins assemble by binding to the secondary cell wall polysaccharide in a manner that requires csaB and tagO
    Justin Kern
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    J Mol Biol 401:757-75. 2010
    ..Together, these results suggest that tagO-mediated assembly of linkage units tethers pyruvylated SCWP to the B. anthracis envelope, thereby enabling S-layer assembly and providing for the pathogenesis of anthrax infections...
  84. ncbi request reprint Targeting proteins to the cell wall of sporulating Bacillus anthracis
    Luciano A Marraffini
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Mol Microbiol 62:1402-17. 2006
    ..anthracis strain Ames. Unlike wild-type bacilli, srtC mutants do not readily form spores in guinea pig tissue or sheep blood unless their vegetative forms are exposed to air...
  85. doi request reprint Sortase as a target of anti-infective therapy
    Anthony W Maresso
    Department of Microbiology, University of Chicago, Chicago, IL 60637, USA
    Pharmacol Rev 60:128-41. 2008
    ..Here we review what is known about sortase catalysis and surface protein function, how surface protein anchoring can be inhibited, and what prospects such inhibition may have for anti-infective therapy...
  86. pmc Staphylococcus aureus mutants with increased lysostaphin resistance
    ANGELIKA GRUNDLING
    Department of Microbiology, University of Chicago, 920 E 58th Street, Chicago, IL 60637, USA
    J Bacteriol 188:6286-97. 2006
    ..Other lyr mutants with intermediate lysostaphin resistance carried bursa aurealis insertions in genes specifying GTP pyrophosphokinase or enzymes of the purine biosynthetic pathway...
  87. pmc Surface proteins and exotoxins are required for the pathogenesis of Staphylococcus aureus pneumonia
    Juliane Bubeck Wardenburg
    Department of Microbiology, University of Chicago, 920 E 58th St, Chicago, IL 60637, USA
    Infect Immun 75:1040-4. 2007
    ..This model closely mimics the clinical and pathological features of pneumonia in human patients. Using this system, we defined a role for S. aureus strain Newman surface proteins and secreted exotoxins in pneumonia-related mortality...
  88. ncbi request reprint Anchor structure of staphylococcal surface proteins. V. Anchor structure of the sortase B substrate IsdC
    Luciano A Marraffini
    Departments of Microbiology and Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA
    J Biol Chem 280:16263-71. 2005
    ..A model is proposed whereby sortases A and B recognize unique features of sorting signals and peptidoglycan substrates to deposit proteins with distinct topologies in the cell wall envelope...
  89. ncbi request reprint Iron acquisition and transport in Staphylococcus aureus
    A W Maresso
    Department of Microbiology, University of Chicago, CLSC Room 601, 920 E 58th Street, Chicago, IL, 60637, USA
    Biometals 19:193-203. 2006
    ..aureus. This review focuses on the molecular attributes of this locus, with emphasis placed on the mechanism of iron transport and the role of such a system during infection...
  90. pmc Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing
    Taeok Bae
    Committee on Microbiology and Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 101:12312-7. 2004
    ..By screening for loss-of-function mutants in a Caenorhabditis elegans killing assay, 71 S. aureus virulence genes were identified. Some of these genes are also required for S. aureus abscess formation in a murine infection model...
  91. pmc Racemic crystallography of synthetic protein enantiomers used to determine the X-ray structure of plectasin by direct methods
    Kalyaneswar Mandal
    Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA
    Protein Sci 18:1146-54. 2009
    ..The structure of the plectasin molecule was well defined for all 40 amino acids and was generally similar to the previously determined NMR structure, suggesting minimal impact of the crystal packing on the plectasin conformation...
  92. ncbi request reprint Protective immunity against plague
    Claire Cornelius
    Department of Microbiology, University of Chicago, IL, USA
    Adv Exp Med Biol 603:415-24. 2007
    ..More research is required to examine the molecular mechanisms of vaccine protection afforded by surface protein antigens and to decipher the host mechanisms responsible for vaccine success...
  93. pmc LcrV plague vaccine with altered immunomodulatory properties
    Katie A Overheim
    Department of Microbiology, University of Chicago, CLSC607B, 920 East 58th Street, Chicago, IL 60637, USA
    Infect Immun 73:5152-9. 2005
    ..Thus, it appears that LcrV variants with reduced immune modulatory properties could be used as a human vaccine to generate protective immunity against plague...
  94. ncbi request reprint Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 E 58th Street, Chicago, IL 60637, USA
    J Biol Chem 277:7447-52. 2002
    ..These results suggest a model whereby sortase forms a thiolate-imidazolium ion pair for the catalysis of its transpeptidation reaction and that the position of tryptophan 194 assists in the formation of this ion pair...
  95. ncbi request reprint An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus
    Peng R Chen
    Department of Chemistry, The University of Chicago, 5735 South Ellis Avenue, Chicago, Illinois 60637, USA
    Nat Chem Biol 2:591-5. 2006
    ..Our study reveals that in S. aureus, MgrA adopts a similar mechanism but uses it to globally regulate different defensive pathways...
  96. ncbi request reprint Substrate recognition by the Yersinia type III protein secretion machinery
    Kumaran S Ramamurthi
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Mol Microbiol 50:1095-102. 2003
    ..This review summarizes recent observations that have been made in this unusual field and proposes models by which proteins may be initiated into this pathway...
  97. pmc Sortases make pili from three ingredients
    So Young Oh
    Department of Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Proc Natl Acad Sci U S A 105:13703-4. 2008
  98. pmc Yersinia enterocolitica type III secretion: yscM1 and yscM2 regulate yop gene expression by a posttranscriptional mechanism that targets the 5' untranslated region of yop mRNA
    Eric D Cambronne
    Committee on Microbiology, University of Chicago, 920 East 85th Street, Chicago, IL 60637, USA
    J Bacteriol 184:5880-93. 2002
    ....
  99. ncbi request reprint Type iii protein secretion in yersinia species
    Kumaran S Ramamurthi
    Committee on Microbiology, University of Chicago, Illinois 60637, USA
    Annu Rev Cell Dev Biol 18:107-33. 2002
    ....
  100. ncbi request reprint Assembly of pili on the surface of Corynebacterium diphtheriae
    Hung Ton-That
    Committee on Microbiology, University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA
    Mol Microbiol 50:1429-38. 2003
    ..This covalent tethering of adjacent pilin subunits appears to have evolved in many Gram-positive pathogens that encode sortase and pilin subunit genes with sorting signals and pilin motifs...

Research Grants48

  1. Vaccine Assembly from Surface Proteins of Staphylococcus Aureus
    Olaf Schneewind; Fiscal Year: 2011
    ..Our research into the development of a staphylococcal vaccine is designed to raise protective immunity in humans and to lower the morbidity and mortality caused by these infections. ..
  2. LcrV Plague Vaccine with Altered Immune Modulatory Properties
    Olaf Schneewind; Fiscal Year: 2007
    ..Research protocols will be designed in close collaboration with FDA-CBER and the entire body of acquired information will be used to develop rV10 vaccine as an Investigative New Drug, permitting its future use in human clinical trials. ..
  3. Biodefense Training in Host-Pathogen Interactions
    Olaf Schneewind; Fiscal Year: 2007
    ..3 respectively. The ultimate goals of our training program are ambitious and aim at establishing a premier research program in biodefense and host-pathogen interactions. ..
  4. Bioterrorism: Molecular Analysis and Intervention
    Olaf Schneewind; Fiscal Year: 2007
    ..abstract_text> ..
  5. Surface Proteins and Sortases of Bacillus anthracis
    Olaf Schneewind; Fiscal Year: 2007
    ..B. anthracis is an important bioterror threat agent and this research proposal will provide future therapeutic and preventive interventions by revealing the underlying biological phenomena of anthrax pathogenesis. ..
  6. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2007
    ..abstract_text> ..
  7. Targeting of Yop Proteins by Yersinia enterocolitica
    Olaf Schneewind; Fiscal Year: 2007
    ..Together these studies will enhance our understanding of the molecular mechanisms that control the establishment of Yersinia infection and may provide insight into future strategies for prevention or therapy of these bacterial diseases. ..
  8. Sortase B-anchored surface proteins of S.aureus
    Olaf Schneewind; Fiscal Year: 2006
    ..Other experiments examine the physiological role of the isd locus in iron transport...
  9. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2006
    ..abstract_text> ..
  10. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2010
    ..By developing inhibitors that prevent the assembly of proteins in the bacterial envelope we aim to develop antiinfectives that may be useful for the treatment of human infections. ..
  11. Yersinia Type III Transport of Yop Proteins into Immune Cells
    Olaf Schneewind; Fiscal Year: 2010
    ..Such knowledge can then be exploited to search for small molecule inhibitors that block the pathogenesis of microbes that rely on type III secretion systems to establish human infectious diseases. ..
  12. Surface Proteins and Sortases of Bacillus anthracis
    Olaf Schneewind; Fiscal Year: 2010
    ..B. anthracis is an important bioterror threat agent and this research proposal will provide future therapeutic and preventive interventions by revealing the underlying biological phenomena of anthrax pathogenesis. ..
  13. Vaccine Assembly from Surface Proteins of Staphylococcus Aureus
    Olaf Schneewind; Fiscal Year: 2010
    ..Our research into the development of a staphylococcal vaccine is designed to raise protective immunity in humans and to lower the morbidity and mortality caused by these infections. ..
  14. Yersinia Type III Transport of Yop Proteins into Immune Cells
    Olaf Schneewind; Fiscal Year: 2009
    ..Such knowledge can then be exploited to search for small molecule inhibitors that block the pathogenesis of microbes that rely on type III secretion systems to establish human infectious diseases. ..
  15. Vaccine Assembly from Surface Proteins of Staphylococcus Aureus
    Olaf Schneewind; Fiscal Year: 2009
    ..Our research into the development of a staphylococcal vaccine is designed to raise protective immunity in humans and to lower the morbidity and mortality caused by these infections. ..
  16. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2009
    ..abstract_text> ..
  17. Surface Proteins and Sortases of Bacillus anthracis
    Olaf Schneewind; Fiscal Year: 2009
    ..B. anthracis is an important bioterror threat agent and this research proposal will provide future therapeutic and preventive interventions by revealing the underlying biological phenomena of anthrax pathogenesis. ..
  18. Targeting of Yop Proteins by Yersinia enterocolitica
    Olaf Schneewind; Fiscal Year: 2006
    ..Together these studies will enhance our understanding of the molecular mechanisms that control the establishment of Yersinia infection and may provide insight into future strategies for prevention or therapy of these bacterial diseases. ..
  19. Sortase B-anchored surface proteins of S.aureus
    Olaf Schneewind; Fiscal Year: 2005
    ..Other experiments examine the physiological role of the isd locus in iron transport...
  20. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2001
    ..aureus, E. faecalis and L. monocytogenes. A genetic screen for S. aureus mutants defective in the retention step of surface protein anchoring will identify missing components of the cell wall sorting machinery. ..
  21. TARGETING OF YOP PROTEIN BY Y ENTEROCOLITICA
    Olaf Schneewind; Fiscal Year: 2001
    ..Furthermore, an in vitro secretion and targeting system will be developed. If successful, the information gained from this research may provide new targets for antimicrobial therapy. ..
  22. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2001
    ..aureus, E. faecalis and L. monocytogenes. A genetic screen for S. aureus mutants defective in the retention step of surface protein anchoring will identify missing components of the cell wall sorting machinery. ..
  23. TARGETING OF YOP PROTEIN BY Y ENTEROCOLITICA
    Olaf Schneewind; Fiscal Year: 2000
    ..Furthermore, an in vitro secretion and targeting system will be developed. If successful, the information gained from this research may provide new targets for antimicrobial therapy. ..
  24. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2000
    ..These in vitro assays will also be used to screen for substances that may inhibit surface protein anchoring at the LPXTG motif and thereby disrupt the pathogenesis of infections caused by Gram- positive bacteria. ..
  25. TARGETING OF YOP PROTEIN BY Y ENTEROCOLITICA
    Olaf Schneewind; Fiscal Year: 1999
    ..Furthermore, an in vitro secretion and targeting system will be developed. If successful, the information gained from this research may provide new targets for antimicrobial therapy. ..
  26. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 1999
    ..These in vitro assays will also be used to screen for substances that may inhibit surface protein anchoring at the LPXTG motif and thereby disrupt the pathogenesis of infections caused by Gram- positive bacteria. ..
  27. TARGETING OF YOP PROTEIN BY Y ENTEROCOLITICA
    Olaf Schneewind; Fiscal Year: 2001
    ..Furthermore, an in vitro secretion and targeting system will be developed. If successful, the information gained from this research may provide new targets for antimicrobial therapy. ..
  28. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2002
    ..aureus, E. faecalis and L. monocytogenes. A genetic screen for S. aureus mutants defective in the retention step of surface protein anchoring will identify missing components of the cell wall sorting machinery. ..
  29. SURFACE PROTEIN ANCHORING IN GRAM POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2005
    ..abstract_text> ..
  30. Targeting of Yop Proteins by Yersinia enterocolitica
    Olaf Schneewind; Fiscal Year: 2005
    ..Together these studies will enhance our understanding of the molecular mechanisms that control the establishment of Yersinia infection and may provide insight into future strategies for prevention or therapy of these bacterial diseases. ..
  31. The Type III Pathway of Yersinia Pestis
    Olaf Schneewind; Fiscal Year: 2004
    ..Together these studies are also aimed at identifying molecular targets for drug therapies, an important area of research to aid in the defense against biological warfare and bioterrorist deployment of the select agent Y. pestis. ..
  32. Sortase B-anchored surface proteins of S.aureus
    Olaf Schneewind; Fiscal Year: 2004
    ..Other experiments examine the physiological role of the isd locus in iron transport...
  33. Targeting of Yop Proteins by Yersinia enterocolitica
    Olaf Schneewind; Fiscal Year: 2004
    ..Together these studies will enhance our understanding of the molecular mechanisms that control the establishment of Yersinia infection and may provide insight into future strategies for prevention or therapy of these bacterial diseases. ..
  34. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2004
    ..aureus, E. faecalis and L. monocytogenes. A genetic screen for S. aureus mutants defective in the retention step of surface protein anchoring will identify missing components of the cell wall sorting machinery. ..
  35. Sortase B-anchored surface proteins of S.aureus
    Olaf Schneewind; Fiscal Year: 2003
    ..Other experiments examine the physiological role of the isd locus in iron transport...
  36. SURFACE PROTEIN ANCHORING IN GRAM-POSITIVE BACTERIA
    Olaf Schneewind; Fiscal Year: 2003
    ..aureus, E. faecalis and L. monocytogenes. A genetic screen for S. aureus mutants defective in the retention step of surface protein anchoring will identify missing components of the cell wall sorting machinery. ..
  37. Sortase B-anchored surface proteins of S.aureus
    Olaf Schneewind; Fiscal Year: 2002
    ..Other experiments examine the physiological role of the isd locus in iron transport...
  38. TARGETING OF YOP PROTEIN BY Y ENTEROCOLITICA
    Olaf Schneewind; Fiscal Year: 2002
    ..Furthermore, an in vitro secretion and targeting system will be developed. If successful, the information gained from this research may provide new targets for antimicrobial therapy. ..