I Schmid

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Biosafety concerns for shared flow cytometry core facilities
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Department of Hematology Oncology, Los Angeles, California 90095, USA
    Cytometry A 56:113-9. 2003
  2. doi request reprint Assessment of telomere length, phenotype, and DNA content
    Ingrid Schmid
    David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
    Curr Protoc Cytom . 2004
  3. pmc How to develop a standard operating procedure for sorting unfixed cells
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Department of Hematology Oncology, Los Angeles, USA
    Methods 57:392-7. 2012
  4. ncbi request reprint Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D
    Ingrid Schmid
    Department of Hematology Oncology, Los Angeles, California 90095, USA
    Nat Protoc 2:187-90. 2007
  5. ncbi request reprint International Society for Analytical Cytology biosafety standard for sorting of unfixed cells
    Ingrid Schmid
    Department of Hematology Oncology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Cytometry A 71:414-37. 2007
  6. ncbi request reprint Simultaneous flow cytometric analysis of two cell surface markers, telomere length, and DNA content
    Ingrid Schmid
    Department of Hematology Oncology, David Geffen School of Medicine, University of California, Los Angeles, California
    Cytometry 49:96-105. 2002
  7. ncbi request reprint Simultaneous flow cytometric measurement of viability and lymphocyte subset proliferation
    I Schmid
    Department of Hematology Oncology, UCLA School of Medicine, 12 236 Factor Building, Los Angeles, CA 90095, USA
    J Immunol Methods 247:175-86. 2001
  8. ncbi request reprint Detection of cell cycle subcompartments by flow cytometric estimation of DNA-RNA content in combination with dual-color immunofluorescence
    I Schmid
    Department of Hematology Oncology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095, USA
    Cytometry 39:108-16. 2000
  9. ncbi request reprint Measurement of lymphocyte subset proliferation by three-color immunofluorescence and DNA flow cytometry
    I Schmid
    UCLA School of Medicine, Department of Hematology Oncology, 12 236 Factor Building, Los Angeles, CA 90095, USA
    J Immunol Methods 235:121-31. 2000
  10. ncbi request reprint Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability
    I Schmid
    Department of Medicine, UCLA, Los Angeles, California, USA
    Cytometry 35:64-74. 1999

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Biosafety concerns for shared flow cytometry core facilities
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Department of Hematology Oncology, Los Angeles, California 90095, USA
    Cytometry A 56:113-9. 2003
    ..In this report we review safety issues that are pertinent to flow cytometry core facilities by discussing the individual components of this biosafety questionnaire...
  2. doi request reprint Assessment of telomere length, phenotype, and DNA content
    Ingrid Schmid
    David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA
    Curr Protoc Cytom . 2004
    ....
  3. pmc How to develop a standard operating procedure for sorting unfixed cells
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Department of Hematology Oncology, Los Angeles, USA
    Methods 57:392-7. 2012
    ....
  4. ncbi request reprint Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D
    Ingrid Schmid
    Department of Hematology Oncology, Los Angeles, California 90095, USA
    Nat Protoc 2:187-90. 2007
    ..Surface antigen staining is carried out according to standard methods before staining for apoptosis. The basic assay can be completed in 30 min, and extra time is needed for cell surface antigen staining...
  5. ncbi request reprint International Society for Analytical Cytology biosafety standard for sorting of unfixed cells
    Ingrid Schmid
    Department of Hematology Oncology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    Cytometry A 71:414-37. 2007
    ..However, the field of cytometry has progressed since 1997, and the document requires an update...
  6. ncbi request reprint Simultaneous flow cytometric analysis of two cell surface markers, telomere length, and DNA content
    Ingrid Schmid
    Department of Hematology Oncology, David Geffen School of Medicine, University of California, Los Angeles, California
    Cytometry 49:96-105. 2002
    ..We overcame these problems and developed a method for measuring telomere length in cell subsets characterized by the expression of two surface antigens...
  7. ncbi request reprint Simultaneous flow cytometric measurement of viability and lymphocyte subset proliferation
    I Schmid
    Department of Hematology Oncology, UCLA School of Medicine, 12 236 Factor Building, Los Angeles, CA 90095, USA
    J Immunol Methods 247:175-86. 2001
    ..We apply this method to the analysis of differential proliferation of lymphocyte subsets in cultures of human peripheral blood mononuclear cells (PBMC) with low viability...
  8. ncbi request reprint Detection of cell cycle subcompartments by flow cytometric estimation of DNA-RNA content in combination with dual-color immunofluorescence
    I Schmid
    Department of Hematology Oncology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095, USA
    Cytometry 39:108-16. 2000
    ..We have developed a method for flow cytometric analysis of DNA-RNA content that has been optimized for simultaneous measurement of dual-color immunofluorescence...
  9. ncbi request reprint Measurement of lymphocyte subset proliferation by three-color immunofluorescence and DNA flow cytometry
    I Schmid
    UCLA School of Medicine, Department of Hematology Oncology, 12 236 Factor Building, Los Angeles, CA 90095, USA
    J Immunol Methods 235:121-31. 2000
    ..Application of the method to the assessment of the differential proliferative responses of lymphocyte subsets of human peripheral blood mononuclear cells (PBMC) that were costimulated with CD3 and with CD28.2 is presented...
  10. ncbi request reprint Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability
    I Schmid
    Department of Medicine, UCLA, Los Angeles, California, USA
    Cytometry 35:64-74. 1999
    ..We developed a method for identification of nonviable cells by fluorescence in cell preparations that are stained simultaneously for cell surface or intracellular immunofluorescence and DNA content...
  11. ncbi request reprint Analysis of DNA content and green fluorescent protein expression
    I Schmid
    UCLA School of Medicine, Los Angeles, California, USA
    Curr Protoc Cytom . 2001
    ..An Alternate Protocol describes a combined GFP fluorescence and cell cycle analysis using unpermeabilized cells stained with the vital dye Hoechst 33342...
  12. ncbi request reprint Testing the efficiency of aerosol containment during cell sorting
    I Schmid
    UCLA School of Medicine, Los Angeles, California, USA
    Curr Protoc Cytom . 2001
    ..Aerosols are detected by plaque formation on susceptible E. coli lawns. With the continuing increase in the sorting of viable human cells, it is vital for cytometrists to be aware of the potential dangers...
  13. ncbi request reprint Assessment of viability, immunofluorescence, and DNA content
    I Schmid
    UCLA School of Medicine, Los Angeles, California, USA
    Curr Protoc Cytom . 2001
    ..Alternate protocols present methods for DNA content and viability assessment and simultaneous surface or intracellular antigen expression...
  14. ncbi request reprint Biosafety guidelines for sorting of unfixed cells
    I Schmid
    Department of Hematology and Oncology, University of California at Los Angeles
    Cytometry 28:99-117. 1997
    ..Recommendations provided here may also help laboratories to obtain institutional and/or regulatory agency approval for sorting of unfixed and known biohazardous samples...
  15. ncbi request reprint Standard safety practices for sorting of unfixed cells
    Ingrid Schmid
    David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    Curr Protoc Cytom . 2007
    ....
  16. ncbi request reprint Biohazard sorting
    Ingrid Schmid
    David Geffen School of Medicine, University of California Los Angeles, Department of Hematology Oncology, Los Angeles, California 90095, USA
    Methods Cell Biol 75:221-40. 2004
  17. pmc Short communication: enhanced CD8+ T cell apoptosis in HIV-infected adolescents with virologic failure on protease inhibitor-based therapy
    Jun Zuo
    David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    AIDS Res Hum Retroviruses 26:681-4. 2010
    ..However, there was a higher degree of apoptosis among viremic patients receiving PI therapy, suggesting an immunologically adverse effect of continuing PI(+) therapy after virological failure...
  18. pmc CREB is a critical regulator of normal hematopoiesis and leukemogenesis
    Jerry C Cheng
    Division of Hematology Oncology, Department of Pediatrics, Gwynne Hazen Cherry Memorial Laboratories and Mattel Children s Hospital, Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA 90095 1752, USA
    Blood 111:1182-92. 2008
    ..Our results suggest that CREB is critical for normal myelopoiesis and leukemia cell proliferation...