Patrick Schlievert

Summary

Affiliation: University of Minnesota
Country: USA

Publications

  1. pmc Glycerol monolaurate inhibits virulence factor production in Bacillus anthracis
    Sara M Vetter
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St, SE, Minneapolis, Minnesota 55455, USA
    Antimicrob Agents Chemother 49:1302-5. 2005
  2. pmc Alpha and beta chains of hemoglobin inhibit production of Staphylococcus aureus exotoxins
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 46:14349-58. 2007
  3. pmc Glycerol monolaurate does not alter rhesus macaque (Macaca mulatta) vaginal lactobacilli and is safe for chronic use
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Antimicrob Agents Chemother 52:4448-54. 2008
  4. pmc Superantigen profile of Staphylococcus aureus isolates from patients with steroid-resistant atopic dermatitis
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Clin Infect Dis 46:1562-7. 2008
  5. pmc Vaginal Staphylococcus aureus superantigen profile shift from 1980 and 1981 to 2003, 2004, and 2005
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street S E, Room 960, Minneapolis, MN 55455, USA
    J Clin Microbiol 45:2704-7. 2007
  6. pmc Secreted virulence factor comparison between methicillin-resistant and methicillin-sensitive Staphylococcus aureus, and its relevance to atopic dermatitis
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Allergy Clin Immunol 125:39-49. 2010
  7. pmc Staphylococcus aureus exotoxins are present in vivo in tampons
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Clin Vaccine Immunol 17:722-7. 2010
  8. pmc Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
    Antimicrob Agents Chemother 51:3056-62. 2007
  9. pmc Cytolysins augment superantigen penetration of stratified mucosa
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 182:2364-73. 2009
  10. pmc Glycerol monolaurate and dodecylglycerol effects on Staphylococcus aureus and toxic shock syndrome toxin-1 in vitro and in vivo
    Ying Chi Lin
    Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota, USA
    PLoS ONE 4:e7499. 2009

Research Grants

  1. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick M Schlievert; Fiscal Year: 2010
  2. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2000
  3. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2006
  4. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2009
  5. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXIN
    Patrick Schlievert; Fiscal Year: 1991

Collaborators

Detail Information

Publications68

  1. pmc Glycerol monolaurate inhibits virulence factor production in Bacillus anthracis
    Sara M Vetter
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St, SE, Minneapolis, Minnesota 55455, USA
    Antimicrob Agents Chemother 49:1302-5. 2005
    ..These data will be useful in developing therapeutic tools to treat anthrax disease, whether in animals or humans. These results also suggest that mechanisms of virulence regulation exist independent of atxA...
  2. pmc Alpha and beta chains of hemoglobin inhibit production of Staphylococcus aureus exotoxins
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 46:14349-58. 2007
    ..The alpha and beta globin chains represent promising molecules to interfere with the pathogenesis of serious staphylococcal diseases...
  3. pmc Glycerol monolaurate does not alter rhesus macaque (Macaca mulatta) vaginal lactobacilli and is safe for chronic use
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Antimicrob Agents Chemother 52:4448-54. 2008
    ..The studies suggest that GML is safe for chronic use in monkeys when applied vaginally; it does not alter either mucosal microflora or integrity...
  4. pmc Superantigen profile of Staphylococcus aureus isolates from patients with steroid-resistant atopic dermatitis
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Clin Infect Dis 46:1562-7. 2008
    ..T cells activated in vitro by superantigens become steroid resistant. The objective was to assess the superantigen profiles of S. aureus isolates from patients with steroid-resistant atopic dermatitis...
  5. pmc Vaginal Staphylococcus aureus superantigen profile shift from 1980 and 1981 to 2003, 2004, and 2005
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street S E, Room 960, Minneapolis, MN 55455, USA
    J Clin Microbiol 45:2704-7. 2007
    ..In 2003 to 2005, more women were colonized than in 1980 and 1981 (23 versus 12%). Enterotoxins G and I and enterotoxin-like superantigens M and N declined, but enterotoxin-like superantigens K, L, and Q increased...
  6. pmc Secreted virulence factor comparison between methicillin-resistant and methicillin-sensitive Staphylococcus aureus, and its relevance to atopic dermatitis
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Allergy Clin Immunol 125:39-49. 2010
    ....
  7. pmc Staphylococcus aureus exotoxins are present in vivo in tampons
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Clin Vaccine Immunol 17:722-7. 2010
    ..The data show that S. aureus exotoxins can be identified in tampons ex vivo in sites with low hemoglobin density...
  8. pmc Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
    Antimicrob Agents Chemother 51:3056-62. 2007
    ..01). The data suggest that use of this growth- and toxin-inhibitory compound may be able to reduce the severity of S. aureus and group A streptococcal mucous membrane and trauma-associated skin infections...
  9. pmc Cytolysins augment superantigen penetration of stratified mucosa
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 182:2364-73. 2009
    ....
  10. pmc Glycerol monolaurate and dodecylglycerol effects on Staphylococcus aureus and toxic shock syndrome toxin-1 in vitro and in vivo
    Ying Chi Lin
    Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, Minnesota, USA
    PLoS ONE 4:e7499. 2009
    ..aureus lipase. Therefore, dodecylglycerol (DDG), a 12 carbon fatty acid monoether, was compared in vitro and in vivo to GML for its effects on S. aureus growth, exotoxin production, and stability...
  11. pmc Characterization of virulence factor regulation by SrrAB, a two-component system in Staphylococcus aureus
    Alexa A Pragman
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Bacteriol 186:2430-8. 2004
    ..Repression of these virulence factors is likely due to a direct interaction between SrrA and the agr, tst, and spa promoters...
  12. pmc Glycerol monolaurate inhibits the effects of Gram-positive select agents on eukaryotic cells
    Marnie L Peterson
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455 0312, USA
    Biochemistry 45:2387-97. 2006
    ..GML may be useful in the management of Gram-positive exotoxin illnesses; its action appears to be membrane stabilization with inhibition of signal transduction...
  13. doi request reprint Reduction in Staphylococcus aureus growth and exotoxin production and in vaginal interleukin 8 levels due to glycerol monolaurate in tampons
    Kristi L Strandberg
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Clin Infect Dis 49:1711-7. 2009
    ..The purpose of this study was to determine whether GML, as a tampon fiber finish, inhibits production of exotoxins and the cytokine interleukin 8 (IL-8) during normal tampon use...
  14. ncbi request reprint Effects of total body irradiation and cyclosporin a on the lethality of toxic shock syndrome toxin-1 in a rabbit model of toxic shock syndrome
    Martin M Dinges
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Infect Dis 188:1142-5. 2003
    ..Nevertheless, these rabbits remained completely susceptible to TSST-1, indicating that TSS can occur in the setting of marked immunosuppression...
  15. ncbi request reprint Functional analysis of the TCR binding domain of toxic shock syndrome toxin-1 predicts further diversity in MHC class II/superantigen/TCR ternary complexes
    John K McCormick
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Immunol 171:1385-92. 2003
    ..1 is probably involved in direct SAG-TCR molecular interactions, possibly contributing to the TCR Vbeta specificity of TSST-1...
  16. ncbi request reprint Molecular analysis of staphylococcal superantigens
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN, USA
    Methods Mol Biol 391:113-26. 2007
    ..We provide a Western immunoblot procedure that allows in vitro quantification of these SAgs...
  17. pmc The innate immune system is activated by stimulation of vaginal epithelial cells with Staphylococcus aureus and toxic shock syndrome toxin 1
    Marnie L Peterson
    Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
    Infect Immun 73:2164-74. 2005
    ..These data suggest innate immune system activation through epithelial cells, reflected in chemokine/cytokine production and influx of lymphocytes, may cause changes in vaginal mucosa permeability, facilitating TSST-1 penetration...
  18. pmc Novel toxic shock syndrome toxin-1 amino acids required for biological activity
    Amanda J Brosnahan
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 47:12995-3003. 2008
    ..Toxic shock syndrome toxin-1 residue D130 may contribute to binding an epithelial receptor, which allows it to penetrate the vaginal mucosa, induce interleukin-8, and cause toxic shock syndrome...
  19. pmc Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence
    Olivia N Chuang
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Infect Immun 77:539-48. 2009
    ....
  20. ncbi request reprint The two-component system Bacillus respiratory response A and B (BrrA-BrrB) is a virulence factor regulator in Bacillus anthracis
    Sara M Vetter
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Biochemistry 46:7343-52. 2007
    ..BrrA-BrrB has sequence similarity with the virulence regulator SrrA-SrrB in Staphylococcus aureus and the aerobic/anaerobic regulator, ResD-ResE, in B. subtilis, and appears to share regulatory mechanisms with ResD-ResE...
  21. pmc Quorum sensing in Staphylococcus infections
    Jeremy M Yarwood
    Department of Microbiology, University of Iowa Roy J and Lucille A Carver College of Medicine, 540 Eckstein Medical Research Building, 500 Newton Road, Iowa City, Iowa 52242, USA
    J Clin Invest 112:1620-5. 2003
    ..There is increasing evidence that the agr phenotype may influence the behavior and pathogenesis of biofilm-associated S. aureus and S. epidermidis and may contribute to the chronic nature of some biofilm-associated infections...
  22. pmc Sequence analysis of the Staphylococcus aureus srrAB loci reveals that truncation of srrA affects growth and virulence factor expression
    Alexa A Pragman
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Bacteriol 189:7515-9. 2007
    ..Two strains (N2 and N19) with N-terminal truncations in SrrA displayed defects in growth and abnormally upregulated virulence factor expression under low-oxygen conditions...
  23. pmc Staphylococcal superantigens cause lethal pulmonary disease in rabbits
    Kristi L Strandberg
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    J Infect Dis 202:1690-7. 2010
    ..aureus (MRSA) are significant causes of serious human infections, including pulmonary illnesses. We investigated the role played by superantigens in lung-associated lethal illness in rabbits...
  24. pmc Reemergence of staphylococcal toxic shock syndrome in Minneapolis-St. Paul, Minnesota, during the 2000-2003 surveillance period
    Patrick M Schlievert
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    J Clin Microbiol 42:2875-6. 2004
  25. pmc Beta toxin catalyzes formation of nucleoprotein matrix in staphylococcal biofilms
    Medora J Huseby
    Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA
    Proc Natl Acad Sci U S A 107:14407-12. 2010
    ..Together, these results suggest that beta toxin cross-linking in the presence of eDNA assists in forming the skeletal framework upon which staphylococcal biofilms are established...
  26. pmc Glycerol monolaurate inhibits Candida and Gardnerella vaginalis in vitro and in vivo but not Lactobacillus
    Kristi L Strandberg
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    Antimicrob Agents Chemother 54:597-601. 2010
    ..No adverse events were reported by participating women. GML is antimicrobial for Candida and G. vaginalis in vitro. Vaginal GML gels in women do not affect Lactobacillus negatively but significantly reduce Candida and G. vaginalis...
  27. ncbi request reprint Staphylococcal alpha-toxin causes increased tracheal epithelial permeability
    James R Phillips
    Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Pediatr Pulmonol 41:1146-52. 2006
    ..Increased P and separation of the tracheal epithelium from the basement membrane occurred after S. aureus alpha-toxin exposure. We conclude that mammalian airway epithelium is a target of S. aureus alpha-toxin...
  28. ncbi request reprint A possible association of recurrent streptococcal infections and acute onset of obsessive-compulsive disorder
    Suck Won Kim
    Department of Psychiatry, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    J Neuropsychiatry Clin Neurosci 16:252-60. 2004
    ..In this article, we review the microbial characteristics of GABHS and the subsequent immune responses to GABHS as a possible etiology of PANDAS...
  29. pmc In vivo induction of virulence and antibiotic resistance transfer in Enterococcus faecalis mediated by the sex pheromone-sensing system of pCF10
    Helmut Hirt
    Department of Microbiology, University of Minnesota, Medical School, Minneapolis, Minnesota 55455, USA
    Infect Immun 70:716-23. 2002
    ..The data suggested that the mechanism of in vivo induction may involve interference of plasma with the normal function of the pheromone peptide and its inhibitor...
  30. ncbi request reprint Virulence regulation in Staphylococcus aureus: the need for in vivo analysis of virulence factor regulation
    Alexa A Pragman
    Department of Microbiology, University of Minnesota Medical School, 960 Mayo Building, MMC 196, 420 Delaware St SE, Minneapolis, MN 55455, USA
    FEMS Immunol Med Microbiol 42:147-54. 2004
    ..In describing the differences between in vitro and in vivo transcription of staphylococcal virulence regulators, we hope to encourage investigators to study virulence regulators using infection models whenever possible...
  31. pmc Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo
    Jeremy M Yarwood
    Department of Microbiology, Medical School, University of Minnesota, Minneapolis, Minnesota, USA
    J Bacteriol 184:1095-101. 2002
    ..These results suggest that agr activation is not necessary for development of staphylococcal TSS and that regulatory circuits responding to the in vivo environment override agr activity...
  32. pmc Characterization of Staphylococcus aureus enterotoxin L
    Paul M Orwin
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Infect Immun 71:2916-9. 2003
    ..T cells bearing the T-cell receptor beta chain variable regions 5.1, 5.2, 6.7, 16, and 22 were significantly stimulated by recombinant SEL...
  33. ncbi request reprint Repression of Staphylococcus aureus SrrAB using inducible antisense srrA alters growth and virulence factor transcript levels
    Alexa A Pragman
    Department of Microbiology, University of Minnesota, Minneapolis, Minnesota 55455, USA
    Biochemistry 46:314-21. 2007
    ..We propose that, under aerobic conditions, SrrAB enhances the levels of tst, spa, and icaR, while under low-oxygen conditions, SrrAB decreases the levels of these three transcripts...
  34. pmc The zinc-dependent major histocompatibility complex class II binding site of streptococcal pyrogenic exotoxin C is critical for maximal superantigen function and toxic activity
    Timothy J Tripp
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA
    Infect Immun 71:1548-50. 2003
    ..Mutation of these SPE C residues resulted in dramatically reduced biological activities. Thus, the zinc-dependent major histocompatibility complex II binding site is critical for maximal biological function of SPE C...
  35. pmc Glycerol monolaurate prevents mucosal SIV transmission
    Qingsheng Li
    Department of Microbiology, Medical School, University of Minnesota, MMC 196, 420 Delaware Street S E, Minneapolis, Minnesota 55455, USA
    Nature 458:1034-8. 2009
    ....
  36. ncbi request reprint Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins
    Paul M Orwin
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, Minnesota 55455, USA
    Biochemistry 41:14033-40. 2002
    ..Recombinant protein stimulated human CD4 and CD8 T cells in a T cell receptor variable region, beta chain (TCRVbeta) specific manner. T cells bearing TCRVbeta 2, 5.1, and 21.3 were significantly stimulated...
  37. pmc Staphylococcal toxic shock syndrome erythroderma is associated with superantigenicity and hypersensitivity
    Chandy C John
    Department of 1Pediatrics, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Clin Infect Dis 49:1893-6. 2009
    ..The associated staphylococcal superantigen was enterotoxin B. We demonstrate that erythroderma depends on preexisting T cell hypersensitivity amplified by superantigenicity...
  38. ncbi request reprint Formation of vegetations during infective endocarditis excludes binding of bacterial-specific host antibodies to Enterococcus faecalis
    John K McCormick
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota, USA
    J Infect Dis 185:994-7. 2002
    ....
  39. ncbi request reprint Characterization and expression analysis of Staphylococcus aureus pathogenicity island 3. Implications for the evolution of staphylococcal pathogenicity islands
    Jeremy M Yarwood
    Department of Microbiology, University of Minnesota Medical School, 420 Delaware Street SE, Minneapolis, MN 55455, USA
    J Biol Chem 277:13138-47. 2002
    ....
  40. pmc Structure and biological activities of beta toxin from Staphylococcus aureus
    Medora Huseby
    Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA
    J Bacteriol 189:8719-26. 2007
    ..Structure-directed active site mutations show that biological activities, including hemolysis and lymphotoxicity, are due to the sphingomyelinase activity of the enzyme...
  41. ncbi request reprint In vivo assessment of human vaginal oxygen and carbon dioxide levels during and post menses
    Donna R Hill
    The FemCare Product Development, Minneapolis, Minnesota, USA
    J Appl Physiol 99:1582-91. 2005
    ....
  42. ncbi request reprint An amino-terminal domain of Enterococcus faecalis aggregation substance is required for aggregation, bacterial internalization by epithelial cells and binding to lipoteichoic acid
    Christopher M Waters
    Department of Microbiology, University of Minnesota Medical School, Minneapolis, MN 55455, USA
    Mol Microbiol 52:1159-71. 2004
    ..Thus, Asc10 possess two distinct domains required for aggregation and eukaryotic cell internalization: an N-terminal domain that promotes binding to LTA and a second domain located near the middle of the protein...
  43. ncbi request reprint Toxic shock-like syndrome associated with staphylococcal enterocolitis in an HIV-infected man
    Donald P Kotler
    Division of Gastroenterology, Department of Medicine and Pathology, St Luke s Roosevelt Hospital Center, New York, NY 10025, USA
    Clin Infect Dis 44:e121-3. 2007
    ..The isolate was methicillin susceptible and produced enterotoxins B and C. This is, to our knowledge, the first reported case of toxic shock syndrome to develop secondary to staphylococcal enterocolitis in an adult...
  44. ncbi request reprint Pyrogenic, lethal, and emetic properties of superantigens in rabbits and primates
    John K McCormick
    Lawson Health Research Institute, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
    Methods Mol Biol 214:245-53. 2003
  45. ncbi request reprint Expression, purification, and detection of novel streptococcal superantigens
    John K McCormick
    Lawson Health Research Institute, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada
    Methods Mol Biol 214:33-43. 2003
  46. pmc Characterization of two novel pyrogenic toxin superantigens made by an acute rheumatic fever clone of Streptococcus pyogenes associated with multiple disease outbreaks
    Laura M Smoot
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 70:7095-104. 2002
    ..The data demonstrate that SpeL and SpeM are pyrogenic toxin superantigens and suggest that they may participate in the host-pathogen interactions in some ARF patients...
  47. ncbi request reprint Penetration of toxic shock syndrome toxin-1 across porcine vaginal mucosa ex vivo: permeability characteristics, toxin distribution, and tissue damage
    Catherine C Davis
    Procter and Gamble Company, Cincinnati, Ohio, USA
    Am J Obstet Gynecol 189:1785-91. 2003
    ..The purpose of this study was to evaluate transvaginal penetration of toxic shock syndrome toxin-1 and its effects on permeability and tissue integrity in vitro with the use of excised porcine vaginal mucosa...
  48. pmc Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence
    Stephen B Beres
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Disease, National Institutes of Health, Hamilton, MT 59840, USA
    Proc Natl Acad Sci U S A 99:10078-83. 2002
    ..Taken together, the results show that phage-mediated recombination has played a critical role in the emergence of a new, unusually virulent clone of serotype M3 GAS...
  49. ncbi request reprint A superantigen hypothesis for the pathogenesis of chronic hyperplastic sinusitis with massive nasal polyposis
    Joel M Bernstein
    Department of Otolaryngology, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, USA
    Am J Rhinol 17:321-6. 2003
    ..However, the initial trigger that causes inflammation of the lateral wall of the nose to up-regulate lymphocytes and eosinophils is still unknown...
  50. ncbi request reprint Prevalence of superantigen-secreting bacteria in patients with Kawasaki disease
    Donald Y M Leung
    Department of Pediatrics, The National Jewish Medical and Research Center and University of Colorado Health Sciences Center, Denver, USA
    J Pediatr 140:742-6. 2002
    ..To assess the prevalence of superantigen secreting bacteria in children with acute Kawasaki disease (KD) relative to control patients...
  51. ncbi request reprint Structures of two streptococcal superantigens bound to TCR beta chains reveal diversity in the architecture of T cell signaling complexes
    Eric J Sundberg
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Structure 10:687-99. 2002
    ..Thus, highly efficient T cell activation may be achieved through structurally diverse strategies of TCR ligation...
  52. ncbi request reprint Purpura fulminans due to Staphylococcus aureus
    Gary R Kravitz
    St Paul Infectious Disease Associates, St Paul, Minnesota, USA
    Clin Infect Dis 40:941-7. 2005
    ....
  53. ncbi request reprint An early favorable outcome of streptococcal toxic shock syndrome may require a combination of antimicrobial and intravenous gamma globulin therapy together with activated protein C
    Lawrence A Cone
    Department of Medicine, Eisenhower Medical Center, Rancho Mirage, CA 92270, USA
    Scand J Infect Dis 38:960-3. 2006
    ..Further studies with this combined approach appear warranted...
  54. pmc Neutralization of multiple staphylococcal superantigens by a single-chain protein consisting of affinity-matured, variable domain repeats
    Xi Yang
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    J Infect Dis 198:344-8. 2008
    ..In the present study, we expressed Vbeta domains in tandem as a single-chain protein and neutralized the clinically important superantigens staphylococcal enterotoxin B and TSS toxin-1 with a single agent...
  55. ncbi request reprint Staphylococcal toxic shock syndrome: still a problem
    Patrick M Schlievert
    Med J Aust 182:651-2. 2005
  56. ncbi request reprint Hospital transmission of community-acquired methicillin-resistant Staphylococcus aureus among postpartum women
    Lisa Saiman
    Department of Pediatrics, Columbia University, New York, New York 10032, USA
    Clin Infect Dis 37:1313-9. 2003
    ..We report that MW2, which was previously limited to the midwestern United States, has spread to the northeastern United States and has become a health care-associated pathogen...
  57. ncbi request reprint The staphylococcal respiratory response regulator SrrAB induces ica gene transcription and polysaccharide intercellular adhesin expression, protecting Staphylococcus aureus from neutrophil killing under anaerobic growth conditions
    Martina Ulrich
    Institute of Medical Microbiology and Hygiene, Universitatsklinikum Tubingen, Germany
    Mol Microbiol 65:1276-87. 2007
    ..These results demonstrate that SrrAB is a major activator of ica expression and PIA production in anaerobic environments, where it contributes to the protection of S. aureus against non-oxidative defence mechanisms...
  58. ncbi request reprint Intravitreally injected human immunoglobulin attenuates the effects of Staphylococcus aureus culture supernatant in a rabbit model of toxin-mediated endophthalmitis
    Stephen L Perkins
    Eye Institute, Medical College of Wisconsin, Milwaukee 53226 4812, USA
    Arch Ophthalmol 122:1499-506. 2004
    ..To determine whether human immunoglobulin attenuates the toxic effects of Staphylococcus aureus culture supernatant in a rabbit model of endophthalmitis...
  59. ncbi request reprint Crystal structure of the streptococcal superantigen SpeI and functional role of a novel loop domain in T cell activation by group V superantigens
    Jean Nicholas P Brouillard
    Department of Microbiology and Immunology, The University of Western Ontario, London, ON, Canada N6A 5B8
    J Mol Biol 367:925-34. 2007
    ....
  60. pmc Genome diversification in Staphylococcus aureus: Molecular evolution of a highly variable chromosomal region encoding the Staphylococcal exotoxin-like family of proteins
    J Ross Fitzgerald
    Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA
    Infect Immun 71:2827-38. 2003
    ..The coexpression of many set genes and the production of multiple SET proteins during human infection suggests an important role in host-pathogen interactions...
  61. ncbi request reprint Toxic shock syndrome in a horse with Staphylococcus aureus pneumonia
    Todd C Holbrook
    Broad Forks Equine Clinic, 230 Sh Rd, Comer, GA 30629, USA
    J Am Vet Med Assoc 222:620-3, 601-2. 2003
    ..The horse's clinical signs were attributed to toxic shock syndrome secondary to pulmonary S. aureus infection...
  62. doi request reprint ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand
    Starchild Weivoda
    Department of Microbiology and Center for Immunology, University of Minnesota, MN 55455, United States
    J Immunol Methods 336:22-9. 2008
    ..Thus, LRG and CRP measurements are non-redundant and indicate different physiological contexts. The ELISA described in this report should be useful to further assess serum LRG as a biomarker for clinical diagnostics...
  63. ncbi request reprint Temperature regulates bacterial protein production: possible role in rosacea
    Mark V Dahl
    Department of Dermatology, Mayo Clinic Scottsdale, Scottsdale, Arizona, USA
    J Am Acad Dermatol 50:266-72. 2004
    ..In conclusion, bacteria isolated from patients with rosacea secrete different proteins and different amounts of protein at different temperatures...
  64. ncbi request reprint Neutralization of staphylococcal enterotoxin B by soluble, high-affinity receptor antagonists
    Rebecca A Buonpane
    Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA
    Nat Med 13:725-9. 2007
    ..These Vbeta proteins represent an easily produced potential treatment for diseases mediated by bacterial superantigens...
  65. ncbi request reprint Recurrent nonmenstrual toxic shock
    Lawrence A Cone
    Clin Infect Dis 34:289. 2002
  66. ncbi request reprint Structural, energetic, and functional analysis of a protein-protein interface at distinct stages of affinity maturation
    Eric J Sundberg
    Center for Advanced Research in Biotechnology, W M Keck Laboratory for Structural Biology, University of Maryland Biotechnology Institute, 9600 Gudelsky Drive, Rockville, MD 20850, USA
    Structure 11:1151-61. 2003
    ..Functional assays reveal how affinity maturation of the SAG-MHC interface corresponds to T cell activation by SAGs...
  67. ncbi request reprint Porcine vagina ex vivo as a model for studying permeability and pathogenesis in mucosa
    Christopher A Squier
    Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, IA 54222, USA
    J Pharm Sci 97:9-21. 2008
    ....

Research Grants21

  1. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick M Schlievert; Fiscal Year: 2010
    ..In vitro characterization of emerging MRSA strains. Specific aim 2b. In vivo characterization of SAg contribution to CA-MRSA pulmonary infections. Specific aim 2c. Characterization of SAg contribution to MRSA endocarditis. ..
  2. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2000
    ..It is hoped in addition to localizing domains required for toxicity, that these studies will clarify important mechanisms of T cell activation and lead to useful toxoid vaccines. ..
  3. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2006
    ....
  4. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXINS
    Patrick Schlievert; Fiscal Year: 2009
    ..In vitro characterization of emerging MRSA strains. Specific aim 2b. In vivo characterization of SAg contribution to CA-MRSA pulmonary infections. Specific aim 2c. Characterization of SAg contribution to MRSA endocarditis. ..
  5. CARDIOTOXICITY OF STREPTOCOCCAL PYROGENIC EXOTOXIN
    Patrick Schlievert; Fiscal Year: 1991
    ..Proteolytic enzymes, other cleavage agents, and genetic techniques will be used to generate toxin fragments with partial biological activities, capacities to bind cells and antibodies...