Randy W Schekman

Summary

Affiliation: University of California
Country: USA

Publications

  1. pmc Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast
    Chao Wen Wang
    Department of Molecular Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 174:973-83. 2006
  2. pmc Biogenesis of gamma-secretase early in the secretory pathway
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 179:951-63. 2007
  3. pmc Role of Sec61p in the ER-associated degradation of short-lived transmembrane proteins
    Daniel C Scott
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 181:1095-105. 2008
  4. pmc A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network
    Yusong Guo
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California Berkeley, Berkeley, United States
    elife 2:e00160. 2013
  5. pmc Sorting signals that mediate traffic of chitin synthase III between the TGN/endosomes and to the plasma membrane in yeast
    Trevor L Starr
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    PLoS ONE 7:e46386. 2012
  6. pmc Multibudded tubules formed by COPII on artificial liposomes
    Kirsten Bacia
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA HALOmem, University of Halle, Kurt Mothes Str 3, 06120 Halle, Germany
    Sci Rep 1:17. 2011
  7. pmc How sterols regulate protein sorting and traffic
    Randy Schekman
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:6496-7. 2007
  8. pmc Charting the secretory pathway in a simple eukaryote
    Randy Schekman
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 21:3781-4. 2010
  9. ncbi request reprint The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins
    Raphael H Valdivia
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Dev Cell 2:283-94. 2002
  10. pmc Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants
    Olivier Deloche
    Howard Hughes Medical Institute, University of California, Berkeley, California 94720 3206, USA
    Mol Biol Cell 13:4296-307. 2002

Research Grants

Collaborators

Detail Information

Publications39

  1. pmc Exomer: A coat complex for transport of select membrane proteins from the trans-Golgi network to the plasma membrane in yeast
    Chao Wen Wang
    Department of Molecular Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 174:973-83. 2006
    ..Unlike other coats (e.g., coat protein complex I, II, and clathrin/adaptor protein complex), the exomer does not form buds or vesicles on liposomes...
  2. pmc Biogenesis of gamma-secretase early in the secretory pathway
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 179:951-63. 2007
    ..Two familial AD (FAD)-linked PS1 variants are inefficiently packaged into transport vesicles generated from the ER. Our results suggest that aberrant trafficking of PS1 may contribute to disease pathology...
  3. pmc Role of Sec61p in the ER-associated degradation of short-lived transmembrane proteins
    Daniel C Scott
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 181:1095-105. 2008
    ..We show that the substrate protein forms a disulfide-linked complex to Sec61p, suggesting that at least part of the retrotranslocation process involves Sec61p...
  4. pmc A novel GTP-binding protein-adaptor protein complex responsible for export of Vangl2 from the trans Golgi network
    Yusong Guo
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California Berkeley, Berkeley, United States
    elife 2:e00160. 2013
    ..DOI:http://dx.doi.org/10.7554/eLife.00160.001...
  5. pmc Sorting signals that mediate traffic of chitin synthase III between the TGN/endosomes and to the plasma membrane in yeast
    Trevor L Starr
    Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    PLoS ONE 7:e46386. 2012
    ..These results establish a role for active protein sorting at the trans-Golgi en route to the plasma membrane (PM) and suggest a possible mechanism to regulate protein trafficking...
  6. pmc Multibudded tubules formed by COPII on artificial liposomes
    Kirsten Bacia
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA HALOmem, University of Halle, Kurt Mothes Str 3, 06120 Halle, Germany
    Sci Rep 1:17. 2011
    ....
  7. pmc How sterols regulate protein sorting and traffic
    Randy Schekman
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 104:6496-7. 2007
  8. pmc Charting the secretory pathway in a simple eukaryote
    Randy Schekman
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 21:3781-4. 2010
    ....
  9. ncbi request reprint The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins
    Raphael H Valdivia
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Dev Cell 2:283-94. 2002
    ..Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN...
  10. pmc Vps10p cycles between the TGN and the late endosome via the plasma membrane in clathrin mutants
    Olivier Deloche
    Howard Hughes Medical Institute, University of California, Berkeley, California 94720 3206, USA
    Mol Biol Cell 13:4296-307. 2002
    ..Once relieved of cargo protein, Vps10p would be recycled to the trans-Golgi and then to the cell surface for further rounds of sorting...
  11. ncbi request reprint Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle
    Marcus C S Lee
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94702, USA
    Cell 122:605-17. 2005
    ..Thus, the initiation of vesicle budding by Sar1p couples the generation of membrane curvature with coat-protein assembly and cargo capture...
  12. ncbi request reprint Bi-directional protein transport between the ER and Golgi
    Marcus C S Lee
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Annu Rev Cell Dev Biol 20:87-123. 2004
    ..We review the mechanisms that govern coat recruitment to the membrane, cargo capture into a transport vesicle, and accurate delivery to the target organelle...
  13. ncbi request reprint Merging cultures in the study of membrane traffic
    Randy Schekman
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, California 94720 3202, USA
    Nat Cell Biol 6:483-6. 2004
    ..The confluence of morphologic, genetic and biochemical approaches laid the foundations for study in this area, and they continue to strengthen our understanding of this essential aspect of cell structure and function...
  14. ncbi request reprint 23 genes, 23 years later
    Randy Schekman
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, California 94720, USA
    Cell 116:S13-5, 1 p following S19. 2004
  15. pmc The exomer coat complex transports Fus1p to the plasma membrane via a novel plasma membrane sorting signal in yeast
    Robyn M Barfield
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA, 94720, USA
    Mol Biol Cell 20:4985-96. 2009
    ..These defects are suppressed by disruption of AP-1. We suggest that IXTPK comprises a novel sorting signal that is recognized and bound by exomer leading to the capture of Fus1p into coated vesicles en route to the cell surface...
  16. ncbi request reprint Lasker Basic Medical Research Award. SEC mutants and the secretory apparatus
    Randy Schekman
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California Berkeley, California, USA
    Nat Med 8:1055-8. 2002
  17. ncbi request reprint Ceramide biosynthesis is required for the formation of the oligomeric H+-ATPase Pma1p in the yeast endoplasmic reticulum
    Marcus C S Lee
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    J Biol Chem 277:22395-401. 2002
    ..We suggest that Lst1p directly conveys Pma1p into a COPII vesicle and that the larger size of mixed Sec24pLst1p COPII vesicles is not essential to the packaging of large oligomeric complexes...
  18. pmc ER-Golgi transport defects are associated with mutations in the Sed5p-binding domain of the COPII coat subunit, Sec24p
    Elizabeth A Miller
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94702, USA
    Mol Biol Cell 16:3719-26. 2005
    ..We propose that the A-site of Sec24p is a multipurpose cargo-binding site that must recognize additional unidentified cargo proteins, at least one of which is essential at a late stage of vesicle fusion...
  19. pmc Concentrative sorting of secretory cargo proteins into COPII-coated vesicles
    Per Malkus
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 159:915-21. 2002
    ..Mutation of a diacidic sequence present in the COOH-terminal cytosolic domain of Gap1p eliminated concentrative sorting of this protein...
  20. ncbi request reprint Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles
    Elizabeth A Miller
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell 114:497-509. 2003
    ..Together, our data support a model whereby Sec24p proteins contain multiple independent cargo binding domains that allow for recognition of a diverse set of sorting signals...
  21. ncbi request reprint Peroxisomes: another branch of the secretory pathway?
    Randy Schekman
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    Cell 122:1-2. 2005
    ..In this issue of Cell, Hoepfner at al. (2005) present the most compelling evidence to date in support of a role for the endoplasmic reticulum in the creation of peroxisomes...
  22. pmc Cargo selection into COPII vesicles is driven by the Sec24p subunit
    Elizabeth Miller
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720 3202, USA
    EMBO J 21:6105-13. 2002
    ..Our data suggest that the principle role of Sec24p is to discriminate cargo molecules for incorporation into COPII vesicles...
  23. pmc A subset of yeast vacuolar protein sorting mutants is blocked in one branch of the exocytic pathway
    Edina Harsay
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    J Cell Biol 156:271-85. 2002
    ..Consistent with this possibility, we show that immunoisolated clathrin-coated vesicles contain invertase...
  24. ncbi request reprint Uncoupled packaging of amyloid precursor protein and presenilin 1 into coat protein complex II vesicles
    Jinoh Kim
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, California 94720, USA
    J Biol Chem 280:7758-68. 2005
    ..Titration of Sarla distinguished the packaging requirements of APP and PS1. Furthermore, APP packaging was not affected by deletion of PS1 or PS1 and 2, suggesting APP and PS1 trafficking from the ER are normally uncoupled...
  25. pmc Role of Vma21p in assembly and transport of the yeast vacuolar ATPase
    Per Malkus
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Mol Biol Cell 15:5075-91. 2004
    ....
  26. pmc Sec16p potentiates the action of COPII proteins to bud transport vesicles
    Frantisek Supek
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California Berkeley, Berkeley, CA 94720, USA
    J Cell Biol 158:1029-38. 2002
    ..We propose that Sec16p nucleates a Sar1-GTP-dependent initiation of COPII assembly and serves to stabilize the coat to premature disassembly after Sar1p hydrolyzes GTP...
  27. pmc The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane
    Raphael H Valdivia
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 100:10287-92. 2003
    ..The mobilization of an intracellular reservoir of Chs3p presents a novel opportunity to investigate the genetic basis of regulated vesicular traffic...
  28. pmc Cargo sorting into multivesicular bodies in vitro
    John H Tran
    Department of Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 106:17395-400. 2009
    ..Biotinylated CPS internalization requires ATP and functional forms of Vps27p and Vps4p and depends on the availability of an exposed lysine residue critical for CPS ubiquitylation...
  29. pmc GTP/GDP exchange by Sec12p enables COPII vesicle bud formation on synthetic liposomes
    Eugene Futai
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    EMBO J 23:4146-55. 2004
    ..We suggest that Sec12p participates actively in the growth of COPII vesicles by charging new Sar1p-GTP molecules that insert at the boundary between a bud and the surrounding endoplasmic reticulum membrane...
  30. doi request reprint Coordination of COPII vesicle trafficking by Sec23
    J Christopher Fromme
    Department of Molecular and Cell Biology, and Howard Hughes Medical Institute, University of California, Berkeley, CA 94720, USA
    Trends Cell Biol 18:330-6. 2008
    ....
  31. pmc The genetic basis of a craniofacial disease provides insight into COPII coat assembly
    J Christopher Fromme
    Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Dev Cell 13:623-34. 2007
    ..Our results indicate that the Sar1-Sec23-Sec24 prebudding complex is sufficient to form cargo-containing tubules in vivo, whereas the Sec13-Sec31 complex is required for membrane fission...
  32. ncbi request reprint COPII-coated vesicles: flexible enough for large cargo?
    J Christopher Fromme
    Department of Molecular and Cell Biology, University of California Berkeley, 628 Barker Hall 3202, Berkeley, CA 94720 3202, USA
    Curr Opin Cell Biol 17:345-52. 2005
    ..COPII is no doubt essential for such transport in vivo, but it remains unclear whether COPII envelops the membrane surrounding large cargo or instead plays a more indirect role in transport carrier biogenesis...
  33. pmc Interaction of Sla2p's ANTH domain with PtdIns(4,5)P2 is important for actin-dependent endocytic internalization
    Yidi Sun
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Mol Biol Cell 16:717-30. 2005
    ..These results demonstrate that the interaction of Sla2p's ANTH domain with PtdIns(4,5)P2 plays a key role in regulation of the dynamics of actin-dependent endocytic internalization...
  34. pmc In vitro reconstitution of ER-stress induced ATF6 transport in COPII vesicles
    Adam J Schindler
    Department of Molecular and Cell Biology and Howard Hughes Medical Institute, Barker Hall, University of California, Berkeley, CA 94720 3202, USA
    Proc Natl Acad Sci U S A 106:17775-80. 2009
    ..These results suggest that unfolded proteins or an ER luminal -SH reactive bond controls BiP-ATF6 stability and access of ATF6 to the COPII budding machinery...
  35. pmc The ins and outs of presenilin 1 membrane topology
    Jinoh Kim
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3203, USA
    Proc Natl Acad Sci U S A 101:905-6. 2004
  36. pmc Ent3p and Ent5p exhibit cargo-specific functions in trafficking proteins between the trans-Golgi network and the endosomes in yeast
    Alenka Copic
    Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California at Berkeley, CA 94720, USA
    Mol Biol Cell 18:1803-15. 2007
    ..We conclude that the clathrin adaptors AP-1, Ent3p, Ent5p, and the Ggas cooperate in different ways to sort proteins between the TGN and the endosomes...
  37. pmc Chs5/6 complex: a multiprotein complex that interacts with and conveys chitin synthase III from the trans-Golgi network to the cell surface
    Siraprapha Sanchatjate
    Department of Molecular and Cell Biology, Howard Hughes Medical Institute, Barker Hall, University of California, Berkeley, Berkeley, CA 94720, USA
    Mol Biol Cell 17:4157-66. 2006
    ..Our results suggest a model of protein sorting at the TGN that involves a peripheral, possibly coat, complex that includes multiple related copies of a specificity determining subunit...
  38. ncbi request reprint Purification and functional properties of yeast Sec12 GEF
    Eugene Futai
    Department of Molecular and Cell Biology, University of California, Berkeley, USA
    Methods Enzymol 404:74-82. 2005
    ..As a result, the entire COPII coat remains stable in the presence of GTP. Here, we describe methods to purify Sec12p, real-time fluorescence assays to evaluate COPII coat formation, and the relevant kinetic analyses...

Research Grants28

  1. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2003
    ..A mechanistic appreciation of the details of vesicle traffic in yeast will also illuminate the pathology of human diseases that impinge on the secretory pathway. ..
  2. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2004
    ..A mechanistic appreciation of the details of vesicle traffic in yeast will also illuminate the pathology of human diseases that impinge on the secretory pathway. ..
  3. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2003
    ..A mechanistic appreciation of the details of vesicle traffic in yeast will also illuminate the pathology of human diseases that impinge on the secretory pathway. ..
  4. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 1999
    ....
  5. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2002
    ..A mechanistic appreciation of the details of vesicle traffic in yeast will also illuminate the pathology of human diseases that impinge on the secretory pathway. ..
  6. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 1991
    ..The secretory mutants that accumulate endoplasmic reticulum at a nonpermissive temperature produce an incompletely glycosylated form of invertase. ..
  7. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 1990
    ..The secretory mutants that accumulate endoplasmic reticulum at a nonpermissive temperature produce an incompletely glycosylated form of invertase. ..
  8. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2001
    ....
  9. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2000
    ....
  10. BIOLOGICAL STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 1980
    ..5. Evaluate the role of the secretory pathway in plasma membrane assembly. Determine which macromolecules continue or fail to be incorporated into the plasma membrane during incubation of sec mutants at 37 degrees C. ..
  11. MOLECULAR STUDIES OF EUKARYOTIC CELL SURFACE GROWTH
    Randy Schekman; Fiscal Year: 2005
    ..A mechanistic appreciation of the details of vesicle traffic in yeast will also illuminate the pathology of human diseases that impinge on the secretory pathway. ..