D V Schaffer

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture
    Randolph S Ashton
    The Howard P Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Biomaterials 28:5518-25. 2007
  2. ncbi request reprint Neurogenesis and neuroadaptation
    David V Schaffer
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Neuromolecular Med 5:1-9. 2004
  3. ncbi request reprint Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery
    D V Schaffer
    Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, MA, USA
    Biotechnol Bioeng 67:598-606. 2000
  4. ncbi request reprint Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery
    D V Schaffer
    Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 273:28004-9. 1998
  5. ncbi request reprint Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles
    James T Koerber
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, California 94720 1462, USA
    Nat Protoc 1:701-6. 2006
  6. pmc Targeted transgene expression in muller glia of normal and diseased retinas using lentiviral vectors
    Kenneth P Greenberg
    Department of Vision Science, University of California Berkeley, Berkeley, CA 94720, USA
    Invest Ophthalmol Vis Sci 48:1844-52. 2007
  7. pmc Retinoschisin gene therapy in photoreceptors, Müller glia or all retinal cells in the Rs1h-/- mouse
    L C Byrne
    1 Department of Molecular and Cellular Biology, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA 2 Department of Chemical and Biomolecular Engineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA 3 Department of Bioengineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA
    Gene Ther 21:585-92. 2014
  8. doi request reprint Enhanced gene delivery to the neonatal retina through systemic administration of tyrosine-mutated AAV9
    D Dalkara
    Department of Chemical Engineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA 94720 3190, USA
    Gene Ther 19:176-81. 2012
  9. doi request reprint Directed evolution of novel adeno-associated viruses for therapeutic gene delivery
    M A Bartel
    Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA
    Gene Ther 19:694-700. 2012
  10. ncbi request reprint Antiviral RNAi therapy: emerging approaches for hitting a moving target
    J N Leonard
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Gene Ther 13:532-40. 2006

Collaborators

Detail Information

Publications57

  1. ncbi request reprint Scaffolds based on degradable alginate hydrogels and poly(lactide-co-glycolide) microspheres for stem cell culture
    Randolph S Ashton
    The Howard P Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
    Biomaterials 28:5518-25. 2007
    ..e. non-degrading, alginate hydrogels. Degradable alginate hydrogels encapsulating stem cells may be widely applied to develop novel therapies for tissue regeneration...
  2. ncbi request reprint Neurogenesis and neuroadaptation
    David V Schaffer
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Neuromolecular Med 5:1-9. 2004
    ....
  3. ncbi request reprint Vector unpacking as a potential barrier for receptor-mediated polyplex gene delivery
    D V Schaffer
    Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, MA, USA
    Biotechnol Bioeng 67:598-606. 2000
    ..This work demonstrates that vector unpackaging should be added to the list of barriers to receptor-mediated polyplex gene delivery, thus providing an additional design principle for targeted synthetic delivery vehicles...
  4. ncbi request reprint Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery
    D V Schaffer
    Department of Chemical Engineering and Biotechnology Process Engineering Center, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 273:28004-9. 1998
    ..Optimizing parameters that affect surface binding therefore improves the efficiency and specificity of molecular conjugate gene delivery...
  5. ncbi request reprint Construction of diverse adeno-associated viral libraries for directed evolution of enhanced gene delivery vehicles
    James T Koerber
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, California 94720 1462, USA
    Nat Protoc 1:701-6. 2006
    ..This procedure can be performed in approximately 2 weeks...
  6. pmc Targeted transgene expression in muller glia of normal and diseased retinas using lentiviral vectors
    Kenneth P Greenberg
    Department of Vision Science, University of California Berkeley, Berkeley, CA 94720, USA
    Invest Ophthalmol Vis Sci 48:1844-52. 2007
    ....
  7. pmc Retinoschisin gene therapy in photoreceptors, Müller glia or all retinal cells in the Rs1h-/- mouse
    L C Byrne
    1 Department of Molecular and Cellular Biology, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA 2 Department of Chemical and Biomolecular Engineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA 3 Department of Bioengineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA
    Gene Ther 21:585-92. 2014
    ..These results collectively point to the importance of cell targeting and appropriate vector choice in the success of retinal gene therapies. ..
  8. doi request reprint Enhanced gene delivery to the neonatal retina through systemic administration of tyrosine-mutated AAV9
    D Dalkara
    Department of Chemical Engineering, The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA 94720 3190, USA
    Gene Ther 19:176-81. 2012
    ..This approach provides a new methodology for the development of retinal gene therapies or creation of animal models of neurodegenerative disease...
  9. doi request reprint Directed evolution of novel adeno-associated viruses for therapeutic gene delivery
    M A Bartel
    Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA
    Gene Ther 19:694-700. 2012
    ..Here we outline progress in creating novel classes of AAV variant libraries and highlight the successful isolation of variants with novel and advantageous in vitro and in vivo gene delivery properties...
  10. ncbi request reprint Antiviral RNAi therapy: emerging approaches for hitting a moving target
    J N Leonard
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Gene Ther 13:532-40. 2006
    ..Here, we review the substantial progress that has been made towards identifying effective antiviral RNAi targets and discuss strategies for translating these findings into effective clinical therapies...
  11. doi request reprint Transposon-based mutagenesis generates diverse adeno-associated viral libraries with novel gene delivery properties
    James T Koerber
    The Department of Chemical Engineering and The Helen Wills Neuroscience Institute, The University of California, Berkeley, CA, USA
    Methods Mol Biol 434:161-70. 2008
    ..The selectable marker is then replaced with a defined oligonucleotide, and the final AAV library is used to package a diverse pool of AAV virions, which can used for functional selection...
  12. pmc Molecular engineering of viral gene delivery vehicles
    David V Schaffer
    The Department of Chemical Engineering, The Department of Bioengineering, and The Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 3220, USA
    Annu Rev Biomed Eng 10:169-94. 2008
    ..Parallel and integrated efforts in rational and library-based design promise to aid the translation of engineered viral vectors toward the clinic...
  13. pmc Molecular evolution of adeno-associated virus for enhanced glial gene delivery
    James T Koerber
    Department of Chemical Engineering, Helen Wills Neuroscience Institute, The University of California, Berkeley, California 94720 1462, USA
    Mol Ther 17:2088-95. 2009
    ....
  14. pmc Inner limiting membrane barriers to AAV-mediated retinal transduction from the vitreous
    Deniz Dalkara
    Department of Chemical Engineering, The University of California at Berkeley, Berkeley, California 94720 3190, USA
    Mol Ther 17:2096-102. 2009
    ..These findings may help avoid limitations, risks, and damage associated with subretinal injections currently necessary for clinical gene therapy...
  15. pmc Relative roles of TGF-beta1 and Wnt in the systemic regulation and aging of satellite cell responses
    Morgan E Carlson
    Department of Bioengineering, University of California Berkeley, Berkeley, CA 94720, USA
    Aging Cell 8:676-89. 2009
    ....
  16. pmc Changes in adeno-associated virus-mediated gene delivery in retinal degeneration
    Kathleen D Kolstad
    Department of Molecular and Cellular Biology and Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, CA 94720 3190, USA
    Hum Gene Ther 21:571-8. 2010
    ..Furthermore, these results indicate that gene delivery to the outer nuclear layer may be achieved by noninvasive intravitreal AAV administration in the diseased state...
  17. ncbi request reprint Gene delivery to the retina using lentiviral vectors
    Kenneth P Greenberg
    Vision Science, University of California, Berkeley, CA 94720, USA
    Adv Exp Med Biol 572:255-66. 2006
  18. pmc Engineering a serum-resistant and thermostable vesicular stomatitis virus G glycoprotein for pseudotyping retroviral and lentiviral vectors
    B Y Hwang
    Chemical and Biomolecular Engineering, Bioengineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 3220, USA
    Gene Ther 20:807-15. 2013
    ..These serum-resistant and thermostable VSV-G variants may aid the application of retroviral and lentiviral vectors to gene therapy. ..
  19. pmc Surface immobilization of hexa-histidine-tagged adeno-associated viral vectors for localized gene delivery
    J H Jang
    Department of Chemical Engineering, The Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Gene Ther 17:1384-9. 2010
    ..This immobilized AAV vector system mediates high-efficiency delivery to cells that contact the surface and thus may have promise for localized gene delivery, which may aid numerous applications of AAV delivery to gene therapy...
  20. ncbi request reprint Directed evolution of adeno-associated virus yields enhanced gene delivery vectors
    Narendra Maheshri
    The Department of Chemical Engineering and The Helen Wills Neuroscience Institute, The University of California, Berkeley, California 94720 1462, USA
    Nat Biotechnol 24:198-204. 2006
    ..This approach, which can be extended to additional gene delivery challenges and serotypes, directs viral evolution to generate 'designer' gene delivery vectors with specified, enhanced properties...
  21. ncbi request reprint PI3K/Akt and CREB regulate adult neural hippocampal progenitor proliferation and differentiation
    Joseph Peltier
    Department of Chemical Engineering, University of California, Berkeley, California 94720, USA
    Dev Neurobiol 67:1348-61. 2007
    ..These results indicate that PI3K/Akt signaling integrates extracellular signaling information to promote cellular proliferation and inhibit differentiation in adult neural progenitors...
  22. pmc Designer gene delivery vectors: molecular engineering and evolution of adeno-associated viral vectors for enhanced gene transfer
    Inchan Kwon
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, 201 Gilman Hall, Berkeley, California 94720 1462, USA
    Pharm Res 25:489-99. 2008
    ..Molecular engineering and directed evolution of AAV vectors therefore offer promise for generating 'designer' gene delivery vectors with enhanced properties...
  23. ncbi request reprint Sonic hedgehog regulates adult neural progenitor proliferation in vitro and in vivo
    Karen Lai
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, 201 Gilman Hall, University of California, Berkeley, California 94720 1462, USA
    Nat Neurosci 6:21-7. 2003
    ..Finally, the pharmacological inhibitor of Shh signaling cyclopamine reduced hippocampal neural progenitor proliferation in vivo. This work identifies Shh as a regulator of adult hippocampal neural stem cells...
  24. ncbi request reprint PEG conjugation moderately protects adeno-associated viral vectors against antibody neutralization
    Gary K Lee
    The Department of Chemical Engineering and, The Helen Wills Neuroscience Institute, The University of California, Berkeley, California 94720, USA
    Biotechnol Bioeng 92:24-34. 2005
    ..However, this first study analyzing the potential of PEG to protect AAV from serum neutralization shows that the approach has promise, which can be further enhanced if the locations of PEG attachment can be more finely controlled...
  25. ncbi request reprint Library selection and directed evolution approaches to engineering targeted viral vectors
    Jae Hyung Jang
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, 201 Gilman Hall, Berkeley, California 94720, USA
    Biotechnol Bioeng 98:515-24. 2007
    ..In addition to creating biomedically useful products, these approaches have future potential to yield novel insights into viral structure-function relationships...
  26. doi request reprint Viral packaging and transduction of adult hippocampal neural progenitors
    Joseph Peltier
    Department of Chemical Engineering, University of California, Berkeley, CA, USA
    Methods Mol Biol 621:103-16. 2010
    ..In this chapter, we describe protocols for retroviral and lentiviral packaging and transduction of progenitors. These techniques are important for studying the role of various genes in progenitor fate choice...
  27. doi request reprint Systems biology approaches to understanding stem cell fate choice
    J Peltier
    University of California, Department of Chemical Engineering, Berkeley, CA 94720 3220, USA
    IET Syst Biol 4:1-11. 2010
    ..Collectively, integrating modelling with experimentation has strong potential for enabling a deeper understanding of stem cell fate choice and thereby aiding the development of therapies to harness stem cells' therapeutic potential...
  28. ncbi request reprint Targeted synthetic gene delivery vectors
    D V Schaffer
    Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA 94720, USA
    Curr Opin Mol Ther 2:155-61. 2000
    ....
  29. pmc DNA shuffling of adeno-associated virus yields functionally diverse viral progeny
    James T Koerber
    Department of Chemical Engineering, University of California at Berkeley, Berkeley, California 94720 1462, USA
    Mol Ther 16:1703-9. 2008
    ..Thus, directed evolution can potentially yield unlimited numbers of new AAV variants with novel gene-delivery properties, and subsequent analysis of these variants can further extend basic knowledge of AAV biology...
  30. pmc Substrate modulus directs neural stem cell behavior
    Krishanu Saha
    Department of Chemical Engineering, Department of Bioengineering, Helen Wills Neuroscience Institute, University of California at Berkeley, Berkeley, California 94720, USA
    Biophys J 95:4426-38. 2008
    ..This work demonstrates that the mechanical and biochemical properties of an aNSC microenvironment can be tuned to regulate the self-renewal and differentiation of aNSCs...
  31. ncbi request reprint Library selection approaches to engineering enhanced retroviral and lentiviral vectors
    Kwang il Lim
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California 94720 3220, USA
    Comb Chem High Throughput Screen 11:111-7. 2008
    ..These high throughput, library-based approaches provide a strong complement to rational engineering of viral components for the rapid development of efficient and safe retroviral and lentiviral vector systems for gene therapy...
  32. ncbi request reprint Neural stem cell engineering: directed differentiation of adult and embryonic stem cells into neurons
    Matthew J Robertson
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA
    Front Biosci 13:21-50. 2008
    ..This review will discuss progress in the identification of signaling mechanisms and culture systems that regulate neural differentiation, neuronal differentiation, and neuronal subtype specification...
  33. pmc Computational and experimental analysis of DNA shuffling
    Narendra Maheshri
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Proc Natl Acad Sci U S A 100:3071-6. 2003
    ..This model will therefore aid in the design of optimal shuffling reaction conditions...
  34. ncbi request reprint Genetic approaches to tissue repair
    David V Schaffer
    University of California Berkeley, 201 Gilman Hall, Mail Stop 1462, Berkeley, CA 94720 1462, USA
    Ann N Y Acad Sci 961:68-70. 2002
  35. ncbi request reprint In situ stem cell therapy: novel targets, familiar challenges
    Smita Agrawal
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, California 94720 1462, USA
    Trends Biotechnol 23:78-83. 2005
    ..Here we review recent progress in the use of genes, proteins and small molecules for in situ stem cell control and manipulation, with a focus on using stem cells of the central nervous system for neuroregeneration...
  36. doi request reprint Isolation of adult hippocampal neural progenitors
    Joseph Peltier
    Department of Chemical Engineering, University of California, Berkeley, CA, USA
    Methods Mol Biol 621:57-63. 2010
    ..Specifically, this chapter details isolation of progenitors from the adult rat for the establishment of in vitro culture...
  37. doi request reprint Transforming growth factor-beta and notch signaling mediate stem cell differentiation into smooth muscle cells
    Kyle Kurpinski
    Joint Graduate Program in Bioengineering, University of California Berkeley, Berkeley, California, USA
    Stem Cells 28:734-42. 2010
    ....
  38. ncbi request reprint The biology and engineering of stem-cell control
    Analeah O'Neill
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 1462, USA
    Biotechnol Appl Biochem 40:5-16. 2004
    ....
  39. doi request reprint In vitro culture and analysis of adult hippocampal neural progenitors
    Joseph Peltier
    Department of Chemical Engineering, University of California, Berkeley, CA, USA
    Methods Mol Biol 621:65-87. 2010
    ..We also describe techniques for analyzing the culture state, such as proliferation and expression of cell fate markers by quantitative RT-PCR and immunofluorescence...
  40. doi request reprint In vivo analysis of engrafted adult hippocampal neural progenitors
    Matthew J Robertson
    Department of Chemical Engineering, University of California, Berkeley, CA, USA
    Methods Mol Biol 621:89-102. 2010
    ..These techniques are important for studying AHNPCs within the physiologically relevant environment of the adult brain rather than in a culture dish...
  41. ncbi request reprint Stochastic gene expression in a lentiviral positive-feedback loop: HIV-1 Tat fluctuations drive phenotypic diversity
    Leor S Weinberger
    Biophysics Graduate Group, The Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA
    Cell 122:169-82. 2005
    ..Thus, Tat stochastics appear sufficient to generate PheB (and potentially proviral latency), illustrating the importance of stochastic fluctuations in gene expression in a mammalian system...
  42. pmc Selection of novel vesicular stomatitis virus glycoprotein variants from a peptide insertion library for enhanced purification of retroviral and lentiviral vectors
    Julie H Yu
    Department of Chemical Engineering, University of California, Berkeley, Berkeley, CA 94720 1462, USA
    J Virol 80:3285-92. 2006
    ..Furthermore, this approach of library generation and selection can readily be extended to other challenges in protein engineering...
  43. ncbi request reprint Advanced targeting strategies for murine retroviral and adeno-associated viral vectors
    Julie H Yu
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, Berkeley 94720, USA
    Adv Biochem Eng Biotechnol 99:147-67. 2005
    ..These two complementary strategies offer the potential to gain enhanced molecular control over vector properties and overcome challenges in generating high titer, stealthy, retargeted vectors...
  44. ncbi request reprint Enhanced preparation of adeno-associated viral vectors by using high hydrostatic pressure to selectively inactivate helper adenovirus
    Joshua N Leonard
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA
    Biotechnol Bioeng 97:1170-9. 2007
    ..Moreover, HHP provides a tool for probing the biophysical properties of AAV, which may facilitate understanding and improving the functions of this important virus...
  45. ncbi request reprint Engineering adeno-associated virus for one-step purification via immobilized metal affinity chromatography
    James T Koerber
    Department of Chemical Engineering and Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, USA
    Hum Gene Ther 18:367-78. 2007
    ..Moreover, a combined rational design and high-throughput library selection approach can aid in the design of enhanced viral gene delivery vectors...
  46. pmc High-throughput, library-based selection of a murine leukemia virus variant to infect nondividing cells
    Julie H Yu
    Department of Chemical Engineering, University of California Berkeley, 201 Gilman Hall, Berkeley, CA 94720 1462, USA
    J Virol 80:8981-8. 2006
    ..Finally, this method of library generation and selection for a desired phenotype directly in a mammalian system can be readily expanded to address other challenges in protein engineering...
  47. pmc Specific insertions of zinc finger domains into Gag-Pol yield engineered retroviral vectors with selective integration properties
    Kwang il Lim
    Department of Chemical Engineering, University of California, 278 Stanley Hall, Berkeley, CA 94720 3220, USA
    Proc Natl Acad Sci U S A 107:12475-80. 2010
    ....
  48. ncbi request reprint Biomimetic interfacial interpenetrating polymer networks control neural stem cell behavior
    Krishanu Saha
    Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA, USA
    J Biomed Mater Res A 81:240-9. 2007
    ..The IPN platform is highly tunable to probe stem cell signal transduction mechanisms and to control stem cell behavior in vitro...
  49. pmc Computational design of antiviral RNA interference strategies that resist human immunodeficiency virus escape
    Joshua N Leonard
    Department of Chemical Engineering and the Helen Wills Neuroscience Institute, University of California, 201 Gilman Hall, Berkeley, CA 94720 1462, USA
    J Virol 79:1645-54. 2005
    ..In addition to providing specific predictions for how to optimize a clinical therapy, this system may also serve as a future tool for investigating more fundamental questions of viral evolution...
  50. ncbi request reprint Immobilized sonic hedgehog N-terminal signaling domain enhances differentiation of bone marrow-derived mesenchymal stem cells
    James E Ho
    Department of Bioengineering, University of California at Berkeley, Berkeley, California 94720 1762, USA
    J Biomed Mater Res A 83:1200-8. 2007
    ....
  51. doi request reprint Engineering strategies to emulate the stem cell niche
    Tandis Vazin
    Department of Chemical Engineering, Department of Bioengineering, and Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720 3220, USA
    Trends Biotechnol 28:117-24. 2010
    ....
  52. pmc Theoretical design of a gene therapy to prevent AIDS but not human immunodeficiency virus type 1 infection
    Leor S Weinberger
    Biophysics Graduate Group Department of Chemical Engineering, University of California Berkeley, Berkeley, CA 94720, USA
    J Virol 77:10028-36. 2003
    ..Thus, the field may benefit by shifting the search for more potent antiviral genes toward engineering optimized therapy viruses that package ultra-efficiently while downregulating viral production moderately...
  53. ncbi request reprint Gene therapy takes a cue from HAART: combinatorial antiviral therapeutics reach the clinic
    Priya S Shah
    Department of Chemical Engineering, University of California, Berkeley, CA 94720 3220, USA
    Sci Transl Med 2:36ps30. 2010
    ....
  54. ncbi request reprint Development of quantitative PCR methods to analyse neural progenitor cell culture state
    Elsa Abranches
    Centro de Engenharia Biologica e Quimica, Instituto Superior Tecnico, Av Rovisco Pais, 1049 001 Lisboa, Portugal
    Biotechnol Appl Biochem 44:1-8. 2006
    ..The present study may aid both basic efforts to study the control of neural stem and progenitor differentiation as well as endeavours to optimize cell culture and expansion conditions for biomedical applications...
  55. pmc Designing synthetic materials to control stem cell phenotype
    Krishanu Saha
    Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA, United States
    Curr Opin Chem Biol 11:381-7. 2007
    ....
  56. ncbi request reprint High-throughput screening of gene function in stem cells using clonal microarrays
    Randolph S Ashton
    The Howard P Isermann Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA
    Stem Cells 25:2928-35. 2007
    ....
  57. pmc Microarraying the cellular microenvironment
    Jae Hyung Jang
    Mol Syst Biol 2:39. 2006