Patricia Y Scaraffia

Summary

Affiliation: University of Arizona
Country: USA

Publications

  1. ncbi request reprint Analysis of whole body ammonia metabolism in Aedes aegypti using [15N]-labeled compounds and mass spectrometry
    Patricia Y Scaraffia
    Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721 0088, USA
    Insect Biochem Mol Biol 36:614-22. 2006
  2. pmc Discovery of an alternate metabolic pathway for urea synthesis in adult Aedes aegypti mosquitoes
    Patricia Y Scaraffia
    Department of Chemistry, University of Arizona, Tucson, AZ 85721 0088, USA
    Proc Natl Acad Sci U S A 105:518-23. 2008
  3. pmc Differential ammonia metabolism in Aedes aegypti fat body and midgut tissues
    Patricia Y Scaraffia
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721 0088, USA
    J Insect Physiol 56:1040-9. 2010
  4. pmc Urea synthesis and excretion in Aedes aegypti mosquitoes are regulated by a unique cross-talk mechanism
    Jun Isoe
    Department of Chemistry and Biochemistry, The Center for Insect Science, The University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 8:e65393. 2013
  5. ncbi request reprint Ammonia metabolism in Aedes aegypti
    Patricia Y Scaraffia
    Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721 0088, USA
    Insect Biochem Mol Biol 35:491-503. 2005
  6. ncbi request reprint Fragmentation pathway for glutamine identification: loss of 73 Da from dimethylformamidine glutamine isobutyl ester
    Qingfen Zhang
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
    J Am Soc Mass Spectrom 16:1192-203. 2005
  7. pmc Low mass MS/MS fragments of protonated amino acids used for distinction of their 13C-isotopomers in metabolic studies
    Xin Ma
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, USA
    J Am Soc Mass Spectrom 24:622-31. 2013
  8. pmc Study of the fragmentation of arginine isobutyl ester applied to arginine quantification in Aedes aegypti mosquito excreta
    David R Bush
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, United States
    J Mass Spectrom 47:1364-71. 2012
  9. pmc Differentiation and quantification of C1 and C2 (13)C-labeled glucose by tandem mass spectrometry
    Wei Jiang
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, 85721, USA
    Anal Biochem 404:40-4. 2010

Collaborators

Detail Information

Publications9

  1. ncbi request reprint Analysis of whole body ammonia metabolism in Aedes aegypti using [15N]-labeled compounds and mass spectrometry
    Patricia Y Scaraffia
    Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721 0088, USA
    Insect Biochem Mol Biol 36:614-22. 2006
    ..Moreover, a complete deduced amino acid sequence for GltS of Ae. aegypti was determined. The sequence analysis revealed that mosquito glutamate synthase belongs to the category of NADH-dependent GltS...
  2. pmc Discovery of an alternate metabolic pathway for urea synthesis in adult Aedes aegypti mosquitoes
    Patricia Y Scaraffia
    Department of Chemistry, University of Arizona, Tucson, AZ 85721 0088, USA
    Proc Natl Acad Sci U S A 105:518-23. 2008
    ..These unexpected findings could lead to the development of metabolism-based strategies for mosquito control...
  3. pmc Differential ammonia metabolism in Aedes aegypti fat body and midgut tissues
    Patricia Y Scaraffia
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721 0088, USA
    J Insect Physiol 56:1040-9. 2010
    ..Instead, midgut cells detoxify ammonia by glutamate dehydrogenase, alanine aminotransferase and GS. These data provide new insights into ammonia metabolism in A. aegypti mosquitoes...
  4. pmc Urea synthesis and excretion in Aedes aegypti mosquitoes are regulated by a unique cross-talk mechanism
    Jun Isoe
    Department of Chemistry and Biochemistry, The Center for Insect Science, The University of Arizona, Tucson, Arizona, United States of America
    PLoS ONE 8:e65393. 2013
    ..This process allows blood-fed mosquitoes to regulate the synthesis and/or excretion of nitrogen waste products, and avoid toxic effects that could result from a lethal concentration of ammonia in their tissues...
  5. ncbi request reprint Ammonia metabolism in Aedes aegypti
    Patricia Y Scaraffia
    Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721 0088, USA
    Insect Biochem Mol Biol 35:491-503. 2005
    ..The mRNA expression patterns of these genes were examined by a real-time RT-PCR in fat body and midgut. The results show that female mosquitoes have evolved efficient mechanisms to detoxify large loads of ammonium/ammonia...
  6. ncbi request reprint Fragmentation pathway for glutamine identification: loss of 73 Da from dimethylformamidine glutamine isobutyl ester
    Qingfen Zhang
    Department of Chemistry, University of Arizona, Tucson, Arizona 85721 0041, USA
    J Am Soc Mass Spectrom 16:1192-203. 2005
    ..It is found that the same method can be used to distinguish aspartic acid from asparagine. This study will improve the application of MS/MS for amino acid quantification and stable isotope labeling metabolism studies...
  7. pmc Low mass MS/MS fragments of protonated amino acids used for distinction of their 13C-isotopomers in metabolic studies
    Xin Ma
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, USA
    J Am Soc Mass Spectrom 24:622-31. 2013
    ..Taken together, the new approach of utilizing low m/z fragments can be applied to distinguish, identify, and quantify (13)C-amino acids labeled at various positions, either in the backbone or side chain...
  8. pmc Study of the fragmentation of arginine isobutyl ester applied to arginine quantification in Aedes aegypti mosquito excreta
    David R Bush
    Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, United States
    J Mass Spectrom 47:1364-71. 2012
    ..This method provides an efficient and rapid tool to quantify Arg in individual blood-fed mosquitoes, and can be applied to other organisms, whose small size severally limits the use of conventional biochemical analysis...
  9. pmc Differentiation and quantification of C1 and C2 (13)C-labeled glucose by tandem mass spectrometry
    Wei Jiang
    Department of Chemistry and Biochemistry, University of Arizona, Tucson, 85721, USA
    Anal Biochem 404:40-4. 2010
    ..The current approach can be applied to individually monitor the metabolic origin and fate of C1 and C2 atoms in (13)C-labeled glucose. This method provides a new means of quantifying glucose isotopomers in metabolic studies...