Patricia Y Scaraffia
Affiliation: University of Arizona
- Analysis of whole body ammonia metabolism in Aedes aegypti using [15N]-labeled compounds and mass spectrometryPatricia Y Scaraffia
Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721 0088, USA
Insect Biochem Mol Biol 36:614-22. 2006..Moreover, a complete deduced amino acid sequence for GltS of Ae. aegypti was determined. The sequence analysis revealed that mosquito glutamate synthase belongs to the category of NADH-dependent GltS...
- Discovery of an alternate metabolic pathway for urea synthesis in adult Aedes aegypti mosquitoesPatricia Y Scaraffia
Department of Chemistry, University of Arizona, Tucson, AZ 85721 0088, USA
Proc Natl Acad Sci U S A 105:518-23. 2008..These unexpected findings could lead to the development of metabolism-based strategies for mosquito control...
- Differential ammonia metabolism in Aedes aegypti fat body and midgut tissuesPatricia Y Scaraffia
Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721 0088, USA
J Insect Physiol 56:1040-9. 2010..Instead, midgut cells detoxify ammonia by glutamate dehydrogenase, alanine aminotransferase and GS. These data provide new insights into ammonia metabolism in A. aegypti mosquitoes...
- Ammonia metabolism in Aedes aegyptiPatricia Y Scaraffia
Department of Biochemistry and Molecular Biophysics, The University of Arizona, Tucson, AZ 85721-0088, USA
Insect Biochem Mol Biol 35:491-503. 2005..The mRNA expression patterns of these genes were examined by a real-time RT-PCR in fat body and midgut. The results show that female mosquitoes have evolved efficient mechanisms to detoxify large loads of ammonium/ammonia...
- Fragmentation pathway for glutamine identification: loss of 73 Da from dimethylformamidine glutamine isobutyl esterQingfen Zhang
Department of Chemistry, University of Arizona, Tucson, Arizona 85721-0041, USA
J Am Soc Mass Spectrom 16:1192-203. 2005..It is found that the same method can be used to distinguish aspartic acid from asparagine. This study will improve the application of MS/MS for amino acid quantification and stable isotope labeling metabolism studies...
- Study of the fragmentation of arginine isobutyl ester applied to arginine quantification in Aedes aegypti mosquito excretaDavid R Bush
Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ 85721, United States
J Mass Spectrom 47:1364-71. 2012..This method provides an efficient and rapid tool to quantify Arg in individual blood-fed mosquitoes, and can be applied to other organisms, whose small size severally limits the use of conventional biochemical analysis...
- Differentiation and quantification of C1 and C2 (13)C-labeled glucose by tandem mass spectrometryWei Jiang
Department of Chemistry and Biochemistry, University of Arizona, Tucson, 85721, USA
Anal Biochem 404:40-4. 2010..The current approach can be applied to individually monitor the metabolic origin and fate of C1 and C2 atoms in (13)C-labeled glucose. This method provides a new means of quantifying glucose isotopomers in metabolic studies...
- Low Mass MS/MS Fragments of Protonated Amino Acids Used for Distinction of Their (13)C- Isotopomers in Metabolic StudiesXin Ma
Department of Chemistry and Biochemistry, The University of Arizona, Tucson, AZ, 85721, USA
J Am Soc Mass Spectrom 24:622-31. 2013..Taken together, the new approach of utilizing low m/z fragments can be applied to distinguish, identify, and quantify (13)C-amino acids labeled at various positions, either in the backbone or side chain...