A Sancar

Summary

Affiliation: University of North Carolina
Country: USA

Publications

  1. ncbi request reprint Nucleotide excision repair
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Prog Nucleic Acid Res Mol Biol 79:183-235. 2005
  2. pmc Comparative photochemistry of animal type 1 and type 4 cryptochromes
    Nuri Ozturk
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 48:8585-93. 2009
  3. pmc Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA
    Jun Hyuk Choi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Nucleic Acids Res 37:1501-9. 2009
  4. pmc Biochemical analysis of the canonical model for the mammalian circadian clock
    Rui Ye
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:25891-902. 2011
  5. pmc Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein
    Tae Hong Kang
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 39:3176-87. 2011
  6. pmc Highly specific and sensitive method for measuring nucleotide excision repair kinetics of ultraviolet photoproducts in human cells
    Jun Hyuk Choi
    Department of Metrology for Quality of Life, Center for Bioanalysis, Korea Research Institute of Standards and Sciences, Daejeon 305 340, South Korea and Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Nucleic Acids Res 42:e29. 2014
  7. pmc A role for cryptochromes in sleep regulation
    Jonathan P Wisor
    Dept of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA
    BMC Neurosci 3:20. 2002
  8. ncbi request reprint Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Annu Rev Biochem 73:39-85. 2004
  9. ncbi request reprint Structure and function of DNA photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB 7260, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Chem Rev 103:2203-37. 2003
  10. ncbi request reprint Regulation of the mammalian circadian clock by cryptochrome
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    J Biol Chem 279:34079-82. 2004

Research Grants

  1. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2007
  2. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2010
  3. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2001
  4. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2000
  5. NUCLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 1993
  6. STRUCTURE AND FUNCTION OF UVRABC EXCISION NUCLEASE
    Aziz Sancar; Fiscal Year: 1991
  7. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2002
  8. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2003
  9. Photolyase/cryptochrome Family, DNA Repair/Circadian Clock
    Aziz Sancar; Fiscal Year: 2009
  10. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2009

Collaborators

Detail Information

Publications125 found, 100 shown here

  1. ncbi request reprint Nucleotide excision repair
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Prog Nucleic Acid Res Mol Biol 79:183-235. 2005
  2. pmc Comparative photochemistry of animal type 1 and type 4 cryptochromes
    Nuri Ozturk
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 48:8585-93. 2009
    ..Finally, we demonstrate that in contrast to animal type 2 CRYs and Arabidopsis CRY1 neither insect type 1 nor type 4 CRYs have autokinase activities...
  3. pmc Cooperative activation of the ATR checkpoint kinase by TopBP1 and damaged DNA
    Jun Hyuk Choi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Nucleic Acids Res 37:1501-9. 2009
    ..Taken together, these findings suggest a cooperative activation mechanism for the ATR checkpoint kinase by TopBP1 and damaged DNA...
  4. pmc Biochemical analysis of the canonical model for the mammalian circadian clock
    Rui Ye
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 286:25891-902. 2011
    ..These findings support some aspects of the canonical model, but also suggest that some key features of the model need to be revised...
  5. pmc Regulation of nucleotide excision repair activity by transcriptional and post-transcriptional control of the XPA protein
    Tae Hong Kang
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 39:3176-87. 2011
    ..Collectively, the data reveal that XPA is a limiting factor in excision repair and that its level is coordinately regulated by the circadian clock, the ubiquitin-proteasome system and DNA damage...
  6. pmc Highly specific and sensitive method for measuring nucleotide excision repair kinetics of ultraviolet photoproducts in human cells
    Jun Hyuk Choi
    Department of Metrology for Quality of Life, Center for Bioanalysis, Korea Research Institute of Standards and Sciences, Daejeon 305 340, South Korea and Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Nucleic Acids Res 42:e29. 2014
    ..We suggest that the new techniques presented here will be a useful and powerful approach for studying the mechanism of human nucleotide excision repair in vivo. ..
  7. pmc A role for cryptochromes in sleep regulation
    Jonathan P Wisor
    Dept of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA, USA
    BMC Neurosci 3:20. 2002
    ..We studied sleep in cry1,2-/- mice under baseline conditions as well as under conditions of constant darkness and enforced wakefulness to determine whether cryptochromes influence sleep regulatory processes...
  8. ncbi request reprint Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Annu Rev Biochem 73:39-85. 2004
    ..In this review the molecular mechanisms of DNA repair and the DNA damage checkpoints in mammalian cells are analyzed...
  9. ncbi request reprint Structure and function of DNA photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building, CB 7260, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Chem Rev 103:2203-37. 2003
  10. ncbi request reprint Regulation of the mammalian circadian clock by cryptochrome
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    J Biol Chem 279:34079-82. 2004
  11. ncbi request reprint Cryptochrome: the second photoactive pigment in the eye and its role in circadian photoreception
    A Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Annu Rev Biochem 69:31-67. 2000
    ..Research on cryptochromes may provide new understanding of human diseases such as seasonal affective disorder and delayed sleep phase syndrome...
  12. pmc Circadian clock control of the cellular response to DNA damage
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    FEBS Lett 584:2618-25. 2010
    ..These new insights are expected to guide development of novel mechanism-based chemotherapeutic regimens...
  13. pmc Purification and characterization of human DNA damage checkpoint Rad complexes
    L A Lindsey-Boltz
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 98:11236-41. 2001
    ..These findings constitute biochemical support for models regarding the roles of checkpoint Rads as damage sensors in the DNA damage checkpoint response of human cells...
  14. pmc Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins
    C H Park
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Proc Natl Acad Sci U S A 91:5017-21. 1994
    ..We conclude that the XPA damage recognition protein makes a ternary complex with the ERCC1/ERCC4(XPF) heterodimer with a potential nuclease function...
  15. ncbi request reprint Role of mouse cryptochrome blue-light photoreceptor in circadian photoresponses
    R J Thresher
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Science 282:1490-4. 1998
    ..These data are consistent with the hypothesis that CRY2 protein modulates circadian responses in mice and suggest that cryptochromes have a role in circadian photoreception in mammals...
  16. ncbi request reprint Human DNA repair excision nuclease. Analysis of the roles of the subunits involved in dual incisions by using anti-XPG and anti-ERCC1 antibodies
    T Matsunaga
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    J Biol Chem 270:20862-9. 1995
    ..Using a defined system for excision repair we also demonstrate that the 3' incision can occur without the 5' incision, leading us to conclude that under certain conditions the two incisions can occur independently...
  17. ncbi request reprint Putative human blue-light photoreceptors hCRY1 and hCRY2 are flavoproteins
    D S Hsu
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Biochemistry 35:13871-7. 1996
    ..We conclude that these newly discovered members of the photolyase/photoreceptor family are not photolyases and instead may function as blue-light photoreceptors in humans...
  18. ncbi request reprint Molecular mechanism of transcription-repair coupling
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Science 260:53-8. 1993
    ..Thus, Mfd appears to target the transcribed strand for repair by recognizing a stalled RNAP and actively recruiting the repair enzyme to the transcription blocking lesion as it dissociates the stalled RNAP...
  19. pmc Transcription-repair coupling and mutation frequency decline
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Bacteriol 175:7509-14. 1993
  20. ncbi request reprint The other function of DNA photolyase: stimulation of excision repair of chemical damage to DNA
    Z Ozer
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Biochemistry 34:15886-9. 1995
    ..coli excision nuclease in vitro. In agreement with the in vitro data, in vivo experiments revealed that photolyase makes cells more resistant to cisplatin killing...
  21. ncbi request reprint Structure and function of transcription-repair coupling factor. II. Catalytic properties
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Biol Chem 270:4890-5. 1995
    ..In vitro, strand-specific repair is not dependent on the MutL and MutS proteins which have recently been implicated in preferential repair in vivo...
  22. ncbi request reprint Circadian regulation of cryptochrome genes in the mouse
    Y Miyamoto
    Department of Biochemistry and Biophysics, Mary Ellen Jones Bldg, CB 7260, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Brain Res Mol Brain Res 71:238-43. 1999
    ..Third, mutation in Cry2 causes a phase delay in Cry1 and mPer1 expression both in the SCN and internal organs relative to wild-type animals. Finally, no obvious periodicity in mCry2 expression was seen in all tissues tested...
  23. ncbi request reprint No "End of History" for photolyases
    A Sancar
    Department of Biochemistry and Biophysics, University of Nor Carolina School of Medicine, Chapel Hill, 27599, USA
    Science 272:48-9. 1996
  24. ncbi request reprint Reaction mechanism of (6-4) photolyase
    X Zhao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 272:32580-90. 1997
    ..Taken together, the data are consistent with photoinduced electron transfer from reduced FAD to substrate, in a manner analogous to the cyclobutane pyrimidine dimer photolyase...
  25. ncbi request reprint Human transcription release factor 2 dissociates RNA polymerases I and II stalled at a cyclobutane thymine dimer
    R Hara
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 274:24779-86. 1999
    ....
  26. doi request reprint Structure and function of animal cryptochromes
    N Ozturk
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Cold Spring Harb Symp Quant Biol 72:119-31. 2007
    ..In mammals, CRYs participate in cell cycle regulation and the cellular response to DNA damage by controlling the expression of some cell cycle genes and by directly interacting with checkpoint proteins...
  27. pmc Cockayne syndrome group B protein enhances elongation by RNA polymerase II
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 94:11205-9. 1997
    ..Thus a deficiency in transcription elongation may contribute to the CS phenotype...
  28. ncbi request reprint Purification and characterization of Drosophila melanogaster photolyase
    S T Kim
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Mutat Res 363:97-104. 1996
    ..We overproduced the Drosophila melanogaster photolyase in Escherichia coli using the cloned gene. The enzyme contains FAD and folate and thus belongs in the folate class of enzymes but with an action spectrum peak at 420 nm...
  29. ncbi request reprint Human transcription-repair coupling factor CSB/ERCC6 is a DNA-stimulated ATPase but is not a helicase and does not disrupt the ternary transcription complex of stalled RNA polymerase II
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 272:1885-90. 1997
    ..CSB is a DNA-binding protein, and it also binds to XPA, TFIIH, and the p34 subunit of TFIIE. These interactions are likely to play a role in recruiting repair proteins to ternary complexes formed at damage sites...
  30. pmc Vitamin B2-based blue-light photoreceptors in the retinohypothalamic tract as the photoactive pigments for setting the circadian clock in mammals
    Y Miyamoto
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 95:6097-102. 1998
    ....
  31. ncbi request reprint Human blue-light photoreceptor hCRY2 specifically interacts with protein serine/threonine phosphatase 5 and modulates its activity
    S Zhao
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599 7260, USA
    Photochem Photobiol 66:727-31. 1997
    ..We found that hCRY2, but not the highly homologous (6-4) photolyase, inhibits the phosphatase activity of PP5. This inhibition may be on the pathway of blue-light signal transduction reaction in humans...
  32. ncbi request reprint The general transcription-repair factor TFIIH is recruited to the excision repair complex by the XPA protein independent of the TFIIE transcription factor
    C H Park
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Biol Chem 270:4896-902. 1995
    ..This latter interaction has no apparent role in general excision repair but may be relevant in the transcription-coupled repair reaction...
  33. pmc Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Proc Natl Acad Sci U S A 88:11574-8. 1991
    ..We conclude that mfd is, most likely, the gene encoding the transcription-repair coupling factor...
  34. pmc Coupling of human circadian and cell cycles by the timeless protein
    Keziban Unsal-Kaçmaz
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building CB 7260, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Mol Cell Biol 25:3109-16. 2005
    ....
  35. pmc Functional redundancy of cryptochromes and classical photoreceptors for nonvisual ocular photoreception in mice
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 97:14697-702. 2000
    ....
  36. ncbi request reprint Post-incision steps of nucleotide excision repair in Escherichia coli. Disassembly of the UvrBC-DNA complex by helicase II and DNA polymerase I
    D K Orren
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Biol Chem 267:780-8. 1992
    ..The discovery of psoralen-UvrB photocross-linking offers the potential of active-site labeling...
  37. ncbi request reprint Characterization of (6-4) photoproduct DNA photolyase
    S T Kim
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599 7260
    J Biol Chem 269:8535-40. 1994
    ..Furthermore, we provide evidence that the (6-4) photoproduct photolyase converts the photoproduct to unmodified bases probably through an oxetane intermediate...
  38. pmc Mechanisms of transcription-repair coupling and mutation frequency decline
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Microbiol Rev 58:317-29. 1994
    ..Thus, the mechanism of strand-specific repair is well understood, but some questions remain regarding the precise mechanism of mutation frequency decline...
  39. pmc Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAK and TFIIH
    J T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Proc Natl Acad Sci U S A 93:6482-7. 1996
    ..These data suggest that TFIIH* and ERCC2/CAK interact to form the TFIIH holoenzyme capable of efficiently assembling the pol II transcription initiation complex and directly participating in excision repair reactions...
  40. ncbi request reprint Structure and function of transcription-repair coupling factor. I. Structural domains and binding properties
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Biol Chem 270:4882-9. 1995
    ..Dissociation of the ternary complex upon hydrolysis of ATP also requires the carboxyl terminus of TRCF. Finally, residues 1-378 bind to UvrA and deliver the damage recognition component of the excision nuclease to the lesion...
  41. ncbi request reprint DNA excision repair
    A Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    Annu Rev Biochem 65:43-81. 1996
    ..Individuals defective in excision repair exhibit a high incidence of cancer while individuals with a defect in coupling transcription to repair suffer from neurological and skeletal abnormalities...
  42. doi request reprint The human ATR-mediated DNA damage checkpoint in a reconstituted system
    Jun Hyuk Choi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, United States
    Methods 48:3-7. 2009
    ..This system provides a powerful tool to gain insight into the molecular mechanism of the ATR pathway. Here we describe preparation of the checkpoint components and our specific kinase assay in more detail...
  43. pmc Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling
    Jun Hyuk Choi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 107:13660-5. 2010
    ..We also find that TopBP1 is recruited to RPA-ssDNA in a manner dependent on ATRIP and that the N terminus of TopBP1 is required for efficient recruitment and activation of ATR kinase...
  44. ncbi request reprint Active site of Escherichia coli DNA photolyase: mutations at Trp277 alter the selectivity of the enzyme without affecting the quantum yield of photorepair
    Y F Li
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Biochemistry 29:5698-706. 1990
    ....
  45. pmc In vitro repair of oxidative DNA damage by human nucleotide excision repair system: possible explanation for neurodegeneration in xeroderma pigmentosum patients
    J T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 94:9463-8. 1997
    ..Our results suggest that XP neurological disease may be caused by defective repair of lesions that are produced in nerve cells by reactive oxygen species generated as by-products of an active oxidative metabolism...
  46. ncbi request reprint Reconstitution of human excision nuclease with recombinant XPF-ERCC1 complex
    T Bessho
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 272:3833-7. 1997
    ....
  47. ncbi request reprint Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease
    T Bessho
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 275:7451-4. 2000
    ..Our results suggest that exonucleolytic processing of primary DNA lesion by hRAD9 may contribute to DNA damage checkpoint response in humans...
  48. ncbi request reprint Characterization of transcription-repair coupling factors in E. coli and humans
    C P Selby
    Department of Biochemistry and Biophysics, University of North Carolina, School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Methods Enzymol 371:300-24. 2003
  49. pmc Reconstitution of a human ATR-mediated checkpoint response to damaged DNA
    Jun Hyuk Choi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 104:13301-6. 2007
    ..This in vitro system provides a useful platform for mechanistic studies of the human DNA damage checkpoint response...
  50. pmc Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress
    Michael G Kemp
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 285:16562-71. 2010
    ..Our results therefore indicate that RPA-covered ssDNA not only supports recruitment and activation of ATR but also, through Tipin and Claspin, it plays an important role in the action of ATR on its critical downstream target Chk1...
  51. ncbi request reprint Purification and characterization of the XPF-ERCC1 complex of human DNA repair excision nuclease
    C H Park
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599, USA
    J Biol Chem 270:22657-60. 1995
    ..This complex also nicks supercoiled DNA weakly, and this nicking activity is stimulated by human replication protein A when the DNA contains UV damage...
  52. ncbi request reprint DNA repair excision nuclease attacks undamaged DNA. A potential source of spontaneous mutations
    M E Branum
    Department of Biochemistry and Biophysics, Mary Ellen Jones Bldg, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    J Biol Chem 276:25421-6. 2001
    ..As resynthesis is invariably associated with mutations, we propose that gratuitous repair may be an important source of spontaneous mutations...
  53. ncbi request reprint Isolation and characterization of functional domains of UvrA
    G M Myles
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Biochemistry 30:3834-40. 1991
    ....
  54. pmc Molecular anatomy of the human excision nuclease assembled at sites of DNA damage
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 22:5938-45. 2002
    ..Based on these results, we propose that RPA70 makes the initial contact with psoralen-damaged DNA but that within preincision complexes, it is RPA32 and XPD that are in close contact with the lesion...
  55. pmc Posttranslational regulation of the mammalian circadian clock by cryptochrome and protein phosphatase 5
    Carrie L Partch
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 103:10467-72. 2006
    ..Collectively, these findings indicate that PP5, CKIepsilon, and cryptochrome dynamically regulate the mammalian circadian clock...
  56. pmc Recruitment of DNA damage checkpoint proteins to damage in transcribed and nontranscribed sequences
    Guochun Jiang
    Department of Biochemistry and Biophysics, Mary Ellen Jones Building CB 7260, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Mol Cell Biol 26:39-49. 2006
    ....
  57. ncbi request reprint Order of assembly of human DNA repair excision nuclease
    M Wakasugi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 274:18759-68. 1999
    ..We conclude that RPA and XPA are the initial damage sensing factors of human excision nuclease...
  58. pmc Preservation of light signaling to the suprachiasmatic nucleus in vitamin A-deficient mice
    C L Thompson
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 98:11708-13. 2001
    ..The data suggest that a non-opsin pigment is the primary circadian photoreceptor in the mouse...
  59. ncbi request reprint Purification and properties of human blue-light photoreceptor cryptochrome 2
    Sezgin Ozgur
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 42:2926-32. 2003
    ..These findings reveal new properties of this protein already known to function as a circadian photoreceptor and a light-independent negative transcriptional regulator of the clock genes...
  60. pmc Repair of DNA-polypeptide crosslinks by human excision nuclease
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 103:4056-61. 2006
    ..Our data suggest that, if coupled with proteolytic degradation of the crosslinked protein, the human excision nuclease may be the major enzyme system for eliminating protein-DNA crosslinks from the genome...
  61. pmc Circadian control of XPA and excision repair of cisplatin-DNA damage by cryptochrome and HERC2 ubiquitin ligase
    Tae Hong Kang
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 107:4890-5. 2010
    ..These findings may be used as a guide for timing of cisplatin chemotherapy...
  62. pmc Reconstitution of human claspin-mediated phosphorylation of Chk1 by the ATR (ataxia telangiectasia-mutated and rad3-related) checkpoint kinase
    Laura A Lindsey-Boltz
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    J Biol Chem 284:33107-14. 2009
    ..This in vitro system recapitulates essential components of the genetically defined ATR-signaling pathway...
  63. ncbi request reprint Overproduction, purification, and characterization of the XPC subunit of the human DNA repair excision nuclease
    J T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 271:19451-6. 1996
    ..Surprisingly, we find that the XPC subunit alone is sufficient for reconstitution of the human excision nuclease and that the HHR23B subunit has no detectable effect on the excision activity of the reconstituted system...
  64. ncbi request reprint Circadian regulation of DNA excision repair: implications for chrono-chemotherapy
    Tae Hong Kang
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Cell Cycle 8:1665-7. 2009
    ..Here we review the significance of the connection linking the circadian clock with nucleotide excision repair and discuss potential implications for chemotherapy...
  65. pmc Preferential binding of ATR protein to UV-damaged DNA
    Keziban Unsal-Kaçmaz
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 99:6673-8. 2002
    ..In addition, damaged DNA stimulates the kinase activity of ATR to a significantly higher level than undamaged DNA. Our data suggest that ATR may function as an initial sensor in the DNA damage checkpoint response...
  66. pmc A mathematical model for human nucleotide excision repair: damage recognition by random order assembly and kinetic proofreading
    Kevin J Kesseler
    Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 7255, USA
    J Theor Biol 249:361-75. 2007
    ..Finally, a comparison between the random order assembly with kinetic proofreading model and a sequential assembly model is made. This investigation reveals the advantages of the random order assembly/kinetic proofreading model...
  67. pmc RNA polymerase: the most specific damage recognition protein in cellular responses to DNA damage?
    Laura A Lindsey-Boltz
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 104:13213-4. 2007
  68. pmc The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement
    Keziban Unsal-Kaçmaz
    Lineberger Comprehensive Cancer Center, CB 7295, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
    Mol Cell Biol 27:3131-42. 2007
    ....
  69. pmc Quaternary structure of ATR and effects of ATRIP and replication protein A on its DNA binding and kinase activities
    Keziban Unsal-Kaçmaz
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 24:1292-300. 2004
    ..These findings suggest that the regulation and mechanism of action of ATR are fundamentally different from those of the other PIKK proteins...
  70. pmc Cdc7-Dbf4 and the human S checkpoint response to UVC
    Timothy P Heffernan
    Department of Pathology and Laboratory Medicine, University of North Carolina Chapel Hill, NC 27599, USA
    J Biol Chem 282:9458-68. 2007
    ..These findings implicate a Dbf4-dependent kinase as a possible target of the ATR- and Chk1-dependent S checkpoint response to UVC...
  71. ncbi request reprint Cryptochrome, circadian cycle, cell cycle checkpoints, and cancer
    Michele A Gauger
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Cancer Res 65:6828-34. 2005
    ..We conclude that the effect of circadian clock disruption on cellular response to DNA damage and cancer predisposition in mice may depend on the mechanism by which the clock is disrupted...
  72. ncbi request reprint Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues
    J J Lin
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    J Biol Chem 267:17688-92. 1992
    ..Our data suggest that UvrC makes the 5' incision by employing a mechanism whereby the three carboxylates acting in concert with H538 and a Mg2+ ion facilitate nucleophilic attack by an active site water molecule...
  73. pmc Cloning, sequencing, expression and characterization of DNA photolyase from Salmonella typhimurium
    Y F Li
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill 27599
    Nucleic Acids Res 19:4885-90. 1991
    ..The binding constant of S.typhimurium photolyase to thymine dimer in DNA is kD = 1.6 x 10(-9) M, and the quantum yield of photorepair at 384 nm is 0.5...
  74. pmc A cryptochrome/photolyase class of enzymes with single-stranded DNA-specific photolyase activity
    Christopher P Selby
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Proc Natl Acad Sci U S A 103:17696-700. 2006
    ..Here, we show that Cry-DASH proteins from bacterial, plant, and animal sources actually are photolyases with high degree of specificity for cyclobutane pyrimidine dimers in ssDNA...
  75. ncbi request reprint Purification and characterization of DNA photolyases
    Gwendolyn B Sancar
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, USA
    Methods Enzymol 408:121-56. 2006
    ....
  76. ncbi request reprint Purification and characterization of Escherichia coli and human nucleotide excision repair enzyme systems
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, USA
    Methods Enzymol 408:189-213. 2006
    ..This chapter outlines methods for expression and purification of these essential repair factors and provides protocols for performing each of the in vitro repair assays with either the E. coli or the human excision nuclease...
  77. ncbi request reprint Repair of DNA-protein cross-links in mammalian cells
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Cell Cycle 5:1366-70. 2006
    ..Repair mechanisms are reasonably well-defined for groups 1 and 3, and suggested for groups 2 and 4. Our work is focused on the recognition and removal of DNA-protein cross-links in duplex DNA (group 4)...
  78. ncbi request reprint Effect of vitamin A depletion on nonvisual phototransduction pathways in cryptochromeless mice
    Carol L Thompson
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA
    J Biol Rhythms 19:504-17. 2004
    ..These data demonstrate that both cryptochromes and opsins regulate nonvisual photoresponses...
  79. pmc The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1
    Wensheng Wang
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA
    Proc Natl Acad Sci U S A 101:16762-7. 2004
    ..This suggests that 9-1-1 is a damage-specific activator of FEN1...
  80. pmc Similar nucleotide excision repair capacity in melanocytes and melanoma cells
    Shobhan Gaddameedhi
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Cancer Res 70:4922-30. 2010
    ..We concluded that melanoma cells retain capacity for nucleotide excision repair, the loss of which probably does not commonly contribute to melanoma progression...
  81. ncbi request reprint Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures
    Funda Sar
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 279:39289-95. 2004
    ....
  82. ncbi request reprint Further evidence for the role of cryptochromes in retinohypothalamic photoreception/phototransduction
    Carol L Thompson
    Department of Biochemistry and Biophysics CB 7260, School of Medicine, University of North Carolina, Chapel Hill, NC 27599 7260, USA
    Brain Res Mol Brain Res 122:158-66. 2004
    ....
  83. ncbi request reprint Photolyase/cryptochrome blue-light photoreceptors use photon energy to repair DNA and reset the circadian clock
    Carol L Thompson
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, NC 27599 7260, USA
    Oncogene 21:9043-56. 2002
    ..Cryptochrome, which has a high degree of sequence identity to photolyase, works as the main circadian photoreceptor and as a component of the molecular clock in animals, including mammals, and regulates growth and development in plants...
  84. pmc The SWI/SNF chromatin-remodeling factor stimulates repair by human excision nuclease in the mononucleosome core particle
    Ryujiro Hara
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, 27599, USA
    Mol Cell Biol 22:6779-87. 2002
    ....
  85. ncbi request reprint Role of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor
    Carrie L Partch
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27599, USA
    Biochemistry 44:3795-805. 2005
    ..Collectively, these findings provide the first biochemical evidence for the proposed conformational rearrangement of cryptochromes upon light exposure...
  86. ncbi request reprint Purification and characterization of three members of the photolyase/cryptochrome family blue-light photoreceptors from Vibrio cholerae
    Erin N Worthington
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 278:39143-54. 2003
    ..In addition, VcCry1 exhibits RNA binding activity and co-purifies with an RNA of 60-70 nucleotides in length...
  87. pmc The human Rad9 checkpoint protein stimulates the carbamoyl phosphate synthetase activity of the multifunctional protein CAD
    Laura A Lindsey-Boltz
    Department of Biochemistry and Biophysics, and Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA
    Nucleic Acids Res 32:4524-30. 2004
    ..Similar results were also obtained with an N-terminal Rad9 fragment. These findings suggest that Rad9 may play a role in ribonucleotide biosynthesis...
  88. pmc Circadian oscillation of nucleotide excision repair in mammalian brain
    Tae Hong Kang
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Proc Natl Acad Sci U S A 106:2864-7. 2009
    ..The circadian oscillation of the repair capacity is caused at least in part by the circadian oscillation of the xeroderma pigmentosum A DNA damage recognition protein...
  89. ncbi request reprint Analysis of the role of intraprotein electron transfer in photoreactivation by DNA photolyase in vivo
    I Halil Kavakli
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 43:15103-10. 2004
    ..We conclude that photoreduction by intraprotein electron transfer is not part of the photolyase photocycle under physiological conditions...
  90. ncbi request reprint Photolyase and cryptochrome blue-light photoreceptors
    Aziz Sancar
    Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599, USA
    Adv Protein Chem 69:73-100. 2004
  91. pmc Purification and characterization of a type III photolyase from Caulobacter crescentus
    Nuri Ozturk
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Biochemistry 47:10255-61. 2008
    ..Using enzyme preparations that contain either both chromophores or only folate, we were able to determine the efficiency and rate of transfer of energy from MTHF to FAD...
  92. ncbi request reprint DNA distress: just ring 9-1-1
    Michael Kemp
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA
    Curr Biol 19:R733-4. 2009
    ....
  93. pmc Effect of damage type on stimulation of human excision nuclease by SWI/SNF chromatin remodeling factor
    Ryujiro Hara
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Mol Cell Biol 23:4121-5. 2003
    ..Equally important, we find that SWI/SNF stimulates the removal of AAF-G and (6-4) photoproduct but not of CPD from nucleosomal DNA. These results shed new light on the low rate of repair of CPDs in human cells in vivo...
  94. ncbi request reprint Photochemistry and photobiology of cryptochrome blue-light photopigments: the search for a photocycle
    Carrie L Partch
    Department of Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA
    Photochem Photobiol 81:1291-304. 2005
    ..In particular, the role of the unique C-terminal domain in cryptochrome phototransduction is discussed...
  95. ncbi request reprint Thermodynamic cooperativity and kinetic proofreading in DNA damage recognition and repair
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599 7260, USA
    Cell Cycle 3:141-4. 2004
    ....
  96. pmc Long patch base excision repair proceeds via coordinated stimulation of the multienzyme DNA repair complex
    Lata Balakrishnan
    Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642, USA
    J Biol Chem 284:15158-72. 2009
    ..Our results imply that this mechanism is still carried out, but in the context of a multienzyme complex that remains structurally intact during the repair process...
  97. pmc Recognition and repair of the cyclobutane thymine dimer, a major cause of skin cancers, by the human excision nuclease
    Joyce T Reardon
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Genes Dev 17:2539-51. 2003
    ....
  98. ncbi request reprint Nucleotide excision repair, oxidative damage, DNA sequence polymorphisms, and cancer treatment
    Stephanie Q Hutsell
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC 27599 7260, USA
    Clin Cancer Res 11:1355-7. 2005
  99. ncbi request reprint Circadian photoreception in humans and mice
    Ibrahim Halil Kavakli
    Department Biochemistry and Biophysics Mary Ellen Jones Building, CB7260 University of North Carolina School of Medicine Chapel Hill, NC 27599, USA
    Mol Interv 2:484-92. 2002
    ..The classical photoreceptors in the outer retina, and melanopsin or other minor opsins in the inner retina, may perform redundant functions in circadian rhythmicity...
  100. ncbi request reprint Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy
    Jack D Griffith
    Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    J Biol Chem 277:15233-6. 2002
    ..Therefore, our results demonstrate structural similarity between the checkpoint Rad complexes and the PCNA and RFC replication factors and thus provide further support for models proposing analogous functions for these complexes...

Research Grants45

  1. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2007
    ..These studies will provide biochemical tests of current checkpoint models that are based on genetic and cellular analyses. ..
  2. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2010
    ....
  3. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2001
    ..The biochemical properties of the proteins involved in this checkpoint response will be studied and an in vitro system for the reconstitution of DNA damage checkpoint pathways will be established. ..
  4. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2000
    ..The biochemical properties of the proteins involved in this checkpoint response will be studied and an in vitro system for the reconstitution of DNA damage checkpoint pathways will be established. ..
  5. NUCLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 1993
    ..e) The XP genes of complementation groups XP-A, -B, -C, -D, and -E will be cloned and the gene products will be overproduced, purified and characterized...
  6. STRUCTURE AND FUNCTION OF UVRABC EXCISION NUCLEASE
    Aziz Sancar; Fiscal Year: 1991
    ..coli DNA ligase. This system will be used to investigate the origin of the short and long repair patches observed in vivo and the involvement of recA protein in long patch repair...
  7. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2002
    ..The biochemical properties of the proteins involved in this checkpoint response will be studied and an in vitro system for the reconstitution of DNA damage checkpoint pathways will be established. ..
  8. NECLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 2003
    ..The biochemical properties of the proteins involved in this checkpoint response will be studied and an in vitro system for the reconstitution of DNA damage checkpoint pathways will be established. ..
  9. Photolyase/cryptochrome Family, DNA Repair/Circadian Clock
    Aziz Sancar; Fiscal Year: 2009
    ..3. We will analyze the light-independent function of cryptochrome in the circadian clock and determine the effects of clock disruption on DNA damage checkpoints at the cellular level and on tumorigenesis in mice. ..
  10. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2009
    ....
  11. Photolyase/cryptochrome Family, DNA Repair/Circadian Clock
    Aziz Sancar; Fiscal Year: 2007
    ..3. We will analyze the light-independent function of cryptochrome in the circadian clock and determine the effects of clock disruption on DNA damage checkpoints at the cellular level and on tumorigenesis in mice. ..
  12. Photolyase/cryptochrome, DNA Repair/Circadian Clock
    Aziz Sancar; Fiscal Year: 2006
    ..3. We will analyze the light-independent function of cryptochrome in the circadian clock and determine the effects of clock disruption on DNA damage checkpoints at the cellular level and on tumorigenesis in mice. ..
  13. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2006
    ..These studies will provide biochemical tests of current checkpoint models that are based on genetic and cellular analyses. ..
  14. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2005
    ..These studies will provide biochemical tests of current checkpoint models that are based on genetic and cellular analyses. ..
  15. STRUCTURE AND FUNCTION OF DNA PHOTOLYASE
    Aziz Sancar; Fiscal Year: 1991
    ....
  16. STRUCTURE AND REGULATION OF E. COLI DNA PHOTOLYASE
    Aziz Sancar; Fiscal Year: 1990
    ..The regulatory sites will be investigated by in vitro and in vivo footprinting techniques...
  17. STRUCTURE AND FUNCTION OF DNA PHOTOLYASE
    Aziz Sancar; Fiscal Year: 1993
    ....
  18. NUCLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 1992
    ..e) The XP genes of complementation groups XP-A, -B, -C, -D, and -E will be cloned and the gene products will be overproduced, purified and characterized...
  19. NUCLEOTIDE EXCISION REPAIR ENZYMES
    Aziz Sancar; Fiscal Year: 1999
    ....
  20. DNA Excision Repair and DNA Damage Checkpoints
    Aziz Sancar; Fiscal Year: 2004
    ..These studies will provide biochemical tests of current checkpoint models that are based on genetic and cellular analyses. ..
  21. STRUCTURE AND FUNCTION OF UVRABC EXCISION NUCLEASE
    Aziz Sancar; Fiscal Year: 1990
    ..coli DNA ligase. This system will be used to investigate the origin of the short and long repair patches observed in vivo and the involvement of recA protein in long patch repair...
  22. STRUCTURE AND FUNCTION OF DNA PHOTOLYASE
    Aziz Sancar; Fiscal Year: 1992
    ....
  23. ROLE OF CRYPTOCHROME IN DNA DAMAGE RESPONSES AND THE CIRCADIAN CLOCK
    Aziz Sancar; Fiscal Year: 2010
    ..We will use genetic and biochemical approaches to understand these regulatory mechanisms and establish a rational basis for chronochemotherapy. ..