Sanjiv Sam Gambhir

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint AMIDE: a free software tool for multimodality medical image analysis
    Andreas Markus Loening
    Stanford University, Department of Radiology and the Bio X Program, 318 Campus Drive, East Wing, 1st Floor, Stanford, CA 94305 542, USA
    Mol Imaging 2:131-7. 2003
  2. pmc Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects: applications to breast cancer
    F Berger
    Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, California, USA
    Breast Cancer Res 3:28-35. 2001
  3. ncbi request reprint Analytical decision model for the cost-effective management of solitary pulmonary nodules
    S S Gambhir
    Crump Institute for Biological Imaging, University of California Los Angeles School of Medicine 90095 1770, USA
    J Clin Oncol 16:2113-25. 1998
  4. ncbi request reprint A new method to estimate parameters of linear compartmental models using artificial neural networks
    S S Gambhir
    The Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095 1770, USA
    Phys Med Biol 43:1659-78. 1998
  5. ncbi request reprint Assays for noninvasive imaging of reporter gene expression
    S S Gambhir
    The Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095 1770, USA
    Nucl Med Biol 26:481-90. 1999
  6. pmc A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography
    S S Gambhir
    The Crump Institute for Biological Imaging, University of California Department of Energy Laboratory of Structural Biology and Molecular Medicine, University of California School of Medicine, Los Angeles, CA 90095 1770, USA
    Proc Natl Acad Sci U S A 97:2785-90. 2000
  7. pmc Imaging transgene expression with radionuclide imaging technologies
    S S Gambhir
    Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095 1770, USA
    Neoplasia 2:118-38. 2000
  8. ncbi request reprint Economic evaluation studies in nuclear medicine: a methodological review of the literature
    S S Gambhir
    Crump Institute for Biological Imaging, UCLA School of Medicine 90095 1770, USA
    Q J Nucl Med 44:121-37. 2000
  9. ncbi request reprint Molecular imaging of cancer with positron emission tomography
    Sanjiv Sam Gambhir
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, 700 Westwood Boulevard, Los Angeles, California 90095 1770, USA
    Nat Rev Cancer 2:683-93. 2002
  10. ncbi request reprint Long-term monitoring of transplanted islets using positron emission tomography
    Yuxin Lu
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Mol Ther 14:851-6. 2006

Collaborators

Detail Information

Publications81

  1. ncbi request reprint AMIDE: a free software tool for multimodality medical image analysis
    Andreas Markus Loening
    Stanford University, Department of Radiology and the Bio X Program, 318 Campus Drive, East Wing, 1st Floor, Stanford, CA 94305 542, USA
    Mol Imaging 2:131-7. 2003
    ..AMIDE runs on UNIX, Macintosh OS X, and Microsoft Windows platforms, and it is freely available with source code under the terms of the GNU General Public License...
  2. pmc Recent advances in imaging endogenous or transferred gene expression utilizing radionuclide technologies in living subjects: applications to breast cancer
    F Berger
    Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, California, USA
    Breast Cancer Res 3:28-35. 2001
    ..Continued development of new technology, probes and assays should help in the better understanding of basic breast cancer biology and in the improved management of breast cancer patients...
  3. ncbi request reprint Analytical decision model for the cost-effective management of solitary pulmonary nodules
    S S Gambhir
    Crump Institute for Biological Imaging, University of California Los Angeles School of Medicine 90095 1770, USA
    J Clin Oncol 16:2113-25. 1998
    ..An incremental cost-effectiveness ratio (ICER) was used to compare all strategies to the wait and watch strategy...
  4. ncbi request reprint A new method to estimate parameters of linear compartmental models using artificial neural networks
    S S Gambhir
    The Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095 1770, USA
    Phys Med Biol 43:1659-78. 1998
    ..These results are primarily due to the inability of weighted nonlinear regression to converge. These results establish that artificial neural networks are powerful tools for estimating parameters for simple compartmental models...
  5. ncbi request reprint Assays for noninvasive imaging of reporter gene expression
    S S Gambhir
    The Crump Institute for Biological Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095 1770, USA
    Nucl Med Biol 26:481-90. 1999
    ..Specific examples utilizing adenoviral-mediated delivery of a reporter gene as well as tumors expressing reporter genes are discussed...
  6. pmc A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography
    S S Gambhir
    The Crump Institute for Biological Imaging, University of California Department of Energy Laboratory of Structural Biology and Molecular Medicine, University of California School of Medicine, Los Angeles, CA 90095 1770, USA
    Proc Natl Acad Sci U S A 97:2785-90. 2000
    ..The use of HSV1-sr39tk as a PET reporter gene and FPCV as a PET reporter probe results in significantly enhanced sensitivity for imaging reporter gene expression in vivo...
  7. pmc Imaging transgene expression with radionuclide imaging technologies
    S S Gambhir
    Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095 1770, USA
    Neoplasia 2:118-38. 2000
    ..We also describe the advantages/disadvantages of each of the assays developed and discuss future animal and human applications...
  8. ncbi request reprint Economic evaluation studies in nuclear medicine: a methodological review of the literature
    S S Gambhir
    Crump Institute for Biological Imaging, UCLA School of Medicine 90095 1770, USA
    Q J Nucl Med 44:121-37. 2000
    ....
  9. ncbi request reprint Molecular imaging of cancer with positron emission tomography
    Sanjiv Sam Gambhir
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, 700 Westwood Boulevard, Los Angeles, California 90095 1770, USA
    Nat Rev Cancer 2:683-93. 2002
    ..PET should become increasingly important in cancer imaging in the next decade...
  10. ncbi request reprint Long-term monitoring of transplanted islets using positron emission tomography
    Yuxin Lu
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Mol Ther 14:851-6. 2006
    ..These studies suggest that PET imaging of lentivirus-transduced islets could provide a safe and feasible method for long-term monitoring of islet graft survival...
  11. pmc Noninvasive imaging of islet grafts using positron-emission tomography
    Yuxin Lu
    Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1735, USA
    Proc Natl Acad Sci U S A 103:11294-9. 2006
    ..These studies lay a foundation for noninvasive quantitative assessments of islet graft survival using PET...
  12. ncbi request reprint Noninvasive imaging of lentiviral-mediated reporter gene expression in living mice
    Abhijit De
    The Crump Institute for Molecular Imaging, Los Angeles, California 90095 1770, USA
    Mol Ther 7:681-91. 2003
    ....
  13. pmc Multimodality imaging of β-cells in mouse models of type 1 and 2 diabetes
    Jing Yong
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles, Los Angeles, California, USA
    Diabetes 60:1383-92. 2011
    ..This should enable the noninvasive imaging of β-cells by charge-coupled device (CCD) and micro-positron emission tomography (PET), as well as the identification of β-cells at the cellular level by fluorescent microscopy...
  14. pmc Optimization of adenoviral vectors to direct highly amplified prostate-specific expression for imaging and gene therapy
    Makoto Sato
    Department of Urology, University of California at Los Angeles School of Medicine, Los Angeles, California 90095, USA
    Mol Ther 8:726-37. 2003
    ..Based on these findings, we hope to refine the TSTA Ads further to improve the efficacy and safety of prostate cancer gene therapy...
  15. ncbi request reprint 124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice
    Gobalakrishnan Sundaresan
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095, USA
    J Nucl Med 44:1962-9. 2003
    ..The present work evaluated the minibody or diabody labeled with (124)I, for imaging tumor-bearing mice using a high-resolution small-animal PET system...
  16. ncbi request reprint Bioluminescent monitoring of islet graft survival after transplantation
    Yuxin Lu
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles School of Medicine, Los Angeles, CA 90095 1735, USA
    Mol Ther 9:428-35. 2004
    ..Such imaging technologies may allow earlier detection of graft rejection and the adjustment of therapies to prolong graft survival posttransplantation...
  17. pmc Functionality of androgen receptor-based gene expression imaging in hormone refractory prostate cancer
    Makoto Sato
    Department of Urology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California, USA
    Clin Cancer Res 11:3743-9. 2005
    ....
  18. ncbi request reprint Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applications
    Tove Olafsen
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095
    Protein Eng Des Sel 17:21-7. 2004
    ..This format will provide a versatile platform for targeting a variety of agents to CEA-positive tumors...
  19. ncbi request reprint Molecular engineering of a two-step transcription amplification (TSTA) system for transgene delivery in prostate cancer
    Liqun Zhang
    Department of Biological Chemistry, University of California, Los Angeles School of Medicine, Los Angeles, California 90095 1737, USA
    Mol Ther 5:223-32. 2002
    ..We discuss the implications of this strategy and its application to molecular imaging and therapy...
  20. ncbi request reprint Positron-emission tomography reporter gene expression imaging in rat myocardium
    Masayuki Inubushi
    Department of Molecular and Medical Pharmacology, The Crump Institute for Molecular Imaging, University of California, Los Angeles 90095 1735, USA
    Circulation 107:326-32. 2003
    ....
  21. ncbi request reprint Positron emission tomography imaging of cardiac reporter gene expression in living rats
    Joseph C Wu
    Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, Calif 90095 1770, USA
    Circulation 106:180-3. 2002
    ..Imaging reporter gene expression is useful for noninvasive monitoring of gene therapy. In this study, we imaged cardiac reporter gene expression in living rats using micro positron emission tomography (microPET)...
  22. pmc A potent, imaging adenoviral vector driven by the cancer-selective mucin-1 promoter that targets breast cancer metastasis
    Steven T Huyn
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles School of Medicine, Los Angeles, California, USA
    Clin Cancer Res 15:3126-34. 2009
    ..Improvements in these areas will play a profound role in reducing mortality from breast cancer...
  23. ncbi request reprint Micro-positron emission tomography imaging of cardiac gene expression in rats using bicistronic adenoviral vector-mediated gene delivery
    Ian Y Chen
    Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, Calif 94305 5427, USA
    Circulation 109:1415-20. 2004
    ....
  24. ncbi request reprint Noninvasive imaging of enhanced prostate-specific gene expression using a two-step transcriptional amplification-based lentivirus vector
    Meera Iyer
    The Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095, USA
    Mol Ther 10:545-52. 2004
    ....
  25. ncbi request reprint CL1-SR39: A noninvasive molecular imaging model of prostate cancer suicide gene therapy using positron emission tomography
    Allan J Pantuck
    Department of Urology, Pharmacology and Crump Institute for Molecular Imaging, University of California School of Medicine, Los Angeles, California, USA
    J Urol 168:1193-8. 2002
    ..We developed a prostate cancer tumor model capable of being noninvasively imaged using positron emission tomography (PET) based on expression of the herpes simplex virus thymidine kinase (HSV1-tk) reporter gene...
  26. ncbi request reprint Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging
    Katy M Venisnik
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Mol Imaging Biol 9:267-77. 2007
    ..4% ID/g) at 21 hours. Although further optimization of this fusion protein may be needed to improve in vivo performance, the diabody-GLDelta15 is a promising optical imaging probe for tumor detection in vivo...
  27. ncbi request reprint Imaging progress of herpes simplex virus type 1 thymidine kinase suicide gene therapy in living subjects with positron emission tomography
    Shahriar S Yaghoubi
    Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, California 90095 1770, USA
    Cancer Gene Ther 12:329-39. 2005
    ..18F]FHBG and [18F]FDG imaging data indicate that exposure of C6sr39 tumors to GCV causes the elimination of [18F]FHBG-accumulating C6sr39 cells and selects for re-growth of tumors unable to accumulate [18F]FHBG...
  28. ncbi request reprint Bioluminescence imaging of systemic tumor targeting using a prostate-specific lentiviral vector
    Meera Iyer
    Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, University of California, Los Angeles, Los Angeles, CA 90095 1770, USA
    Hum Gene Ther 17:125-32. 2006
    ..Noninvasive imaging using such vectors should be useful for monitoring long-term gene expression in gene therapy applications...
  29. ncbi request reprint Imaging androgen receptor function during flutamide treatment in the LAPC9 xenograft model
    Romyla Ilagan
    Department of Biological Chemistry, School of Medicine, University of California at Los Angeles, 10833 Le Conte Avenue, CHS 33 142, Los Angeles, California 90095 1737, USA
    Mol Cancer Ther 4:1662-9. 2005
    ..The application of imaging technology to study animal models of cancer provides mechanistic insight into antiandrogen targeting of androgen receptor during disease progression...
  30. pmc Small animal imaging center design: the facility at the UCLA Crump Institute for Molecular Imaging
    David B Stout
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, The David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Mol Imaging Biol 7:393-402. 2005
    ....
  31. pmc Noninvasive imaging of therapeutic gene expression using a bidirectional transcriptional amplification strategy
    Sunetra Ray
    Department of Molecular and Medical Pharmacology, University of California at Los Angeles UCLA School of Medicine, Los Angeles, California, USA
    Mol Ther 16:1848-56. 2008
    ..These studies demonstrate the potential of the bidirectional TSTA system to achieve high levels of gene expression from a weak promoter, while preserving specificity and the ability to image expression of the TG noninvasively...
  32. ncbi request reprint Optimizing radiolabeled engineered anti-p185HER2 antibody fragments for in vivo imaging
    Tove Olafsen
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at University of California at Los Angeles, 90095, USA
    Cancer Res 65:5907-16. 2005
    ..1 +/- 1.5% ID/g). Thus, by manipulating the size and format of anti-p185(HER2) antibody fragments, the kidney activity was reduced and high or low expression of p185HER2 in xenografts could be distinguished by microPET imaging...
  33. ncbi request reprint Molecular imaging of the kinetics of vascular endothelial growth factor gene expression in ischemic myocardium
    Joseph C Wu
    Department of Molecular and Medical Pharmacology, the Crump Institute of Molecular Imaging, UCLA School of Medicine, Los Angeles, Calif, USA
    Circulation 110:685-91. 2004
    ..In this study, we used micro-positron emission tomography (microPET) to monitor the transgene expression, function, and effects in a whole-body system...
  34. ncbi request reprint Noninvasive imaging of ex vivo intracoronarily delivered nonviral therapeutic transgene expression in heart
    Luyi Sen
    Division of Cardiothoracic Surgery, Department of Surgery, UCLA Medical Center, David Geffen School of Medicine, University of California at Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA
    Mol Ther 12:49-57. 2005
    ..Our results demonstrate for the first time that PET reporter-therapeutic linked gene imaging is applicable for noninvasively monitoring ex vivo intracoronarily delivered therapeutic transgene expression in the whole heart...
  35. ncbi request reprint A tracer kinetic model for 18F-FHBG for quantitating herpes simplex virus type 1 thymidine kinase reporter gene expression in living animals using PET
    Leeta Alison Green
    The Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Nucl Med 45:1560-70. 2004
    ..We therefore investigated tracer kinetic models for 18F-FHBG dynamic microPET data and noninvasive methods for determining blood time-activity curves in an adenoviral gene delivery model in mice...
  36. ncbi request reprint Evaluating early dementia with and without assessment of regional cerebral metabolism by PET: a comparison of predicted costs and benefits
    Daniel H S Silverman
    Department of Molecular and Medical Pharmacology and Ahmanson Biological Imaging Center, UCLA School of Medicine, University of California, Los Angeles 90095 6942, USA
    J Nucl Med 43:253-66. 2002
    ..The precise diagnostic role of PET and its economic impact in this context, however, have not been systematically examined previously...
  37. ncbi request reprint Synthesis of a new heterobifunctional linker, N-[4-(aminooxy)butyl]maleimide, for facile access to a thiol-reactive 18F-labeling agent
    Tatsushi Toyokuni
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, California 90095 1770, USA
    Bioconjug Chem 14:1253-9. 2003
    ....
  38. ncbi request reprint Optimizing prostate cancer suicide gene therapy using herpes simplex virus thymidine kinase active site variants
    Allan J Pantuck
    Department of Urology, School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
    Hum Gene Ther 13:777-89. 2002
    ..Both in vitro and in vivo studies suggest improved killing with the sr39tk variant. Thus, the results suggest that the use of sr39tk in future trials of prostate cancer tk suicide gene therapy may be beneficial...
  39. ncbi request reprint MicroPET imaging of prostate cancer in LNCAP-SR39TK-GFP mouse xenografts
    Honghao Yang
    Department of Medicine, Division of Hematology Oncology, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90095, USA
    Prostate 55:39-47. 2003
    ..The aim of this study was to develop models that allow serial, noninvasive imaging of human prostate cancer cells in immunodeficient mice using a dedicated small animal positron emission tomography scanner (microPET)...
  40. pmc Ex vivo cell labeling with 64Cu-pyruvaldehyde-bis(N4-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
    Nona Adonai
    The Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, CA 90095 1770, USA
    Proc Natl Acad Sci U S A 99:3030-5. 2002
    ..Given the longer t(1/2) of 64Cu (12.7 h) relative to 18F (110 min), longer cell-tracking periods (up to 24-36 h) should be possible now with PET...
  41. ncbi request reprint Imaging tri-fusion multimodality reporter gene expression in living subjects
    Pritha Ray
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
    Cancer Res 64:1323-30. 2004
    ..Imaging of reporter gene expression from single cells to living animals with the help of a single tri-fusion reporter gene will have the potential to accelerate translational cancer research...
  42. ncbi request reprint Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors
    Katy M Venisnik
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA
    Protein Eng Des Sel 19:453-60. 2006
    ....
  43. ncbi request reprint Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene
    Young J Kim
    Division of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Mol Imaging Biol 6:331-40. 2004
    ..Using the adoptive transfer model, tumor-specific lymphocytic migration was detected by both microPET scan and bioluminescence imaging...
  44. ncbi request reprint Optical imaging of cardiac reporter gene expression in living rats
    Joseph C Wu
    Crump Institute for Molecular Imaging and Department of Medicine, Division of Cardiology, UCLA School of Medicine, Los Angeles, Calif, USA
    Circulation 105:1631-4. 2002
    ..Noninvasive imaging of the temporal and spatial characteristics of cardiac gene expression in the same subject offers significant advantages...
  45. ncbi request reprint Interrogating androgen receptor function in recurrent prostate cancer
    Liqun Zhang
    Departments of Biological Chemistry, University of California Los Angeles School of Medicine, Los Angeles, CA 90095, USA
    Cancer Res 63:4552-60. 2003
    ..Our data support the concept that AR is fully functional in recurrent cancer and suggest a model by which a poised but largely inactive transcription complex facilitates reactivation by AR at castrate levels of ligand...
  46. ncbi request reprint Applications of molecular imaging in cancer gene therapy
    Meera Iyer
    Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles 90095, USA
    Curr Gene Ther 5:607-18. 2005
    ..Coupling PET to gene therapy of cancer has already been implemented in several clinical studies. This approach would help to improve the efficacy and safety of future gene therapy clinical trials...
  47. ncbi request reprint Comparison of [18F]FHBG and [14C]FIAU for imaging of HSV1-tk reporter gene expression: adenoviral infection vs stable transfection
    Jung Jun Min
    The Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095 1770, USA
    Eur J Nucl Med Mol Imaging 30:1547-60. 2003
    ..g., thymidine) when cells are infected with adenovirus. For adenoviral studies, the [(18)F]FHBG/ HSV1-sr39tk combination was shown to be more sensitive than the [(14)C]FIAU/ HSV1-tk combination HSV1-tk...
  48. ncbi request reprint Molecular imaging of cardiac cell transplantation in living animals using optical bioluminescence and positron emission tomography
    Joseph C Wu
    Department of Molecular and Medical Pharmacology and Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, Calif, USA
    Circulation 108:1302-5. 2003
    ..The current method of analyzing myocardial cell transplantation relies on postmortem histology. We sought to demonstrate the feasibility of monitoring transplanted cell survival in living animals using molecular imaging techniques...
  49. ncbi request reprint Whole-body skeletal imaging in mice utilizing microPET: optimization of reproducibility and applications in animal models of bone disease
    Frank Berger
    The Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology University of California School of Medicine, 700 Westwood Blvd, Los Angeles, CA 90095, USA
    Eur J Nucl Med Mol Imaging 29:1225-36. 2002
    ..Morphological imaging with microCAT is useful to display correlative changes in anatomy. Detailed in vivo studies of the murine skeleton in various small animal models of bone diseases should now be possible...
  50. ncbi request reprint Molecular imaging of homodimeric protein-protein interactions in living subjects
    Tarik F Massoud
    The Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA
    FASEB J 18:1105-7. 2004
    ....
  51. ncbi request reprint Imaging mitogen-activated protein kinase function in xenograft models of prostate cancer
    Romyla Ilagan
    Department of Biological Chemistry, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California, USA
    Cancer Res 66:10778-85. 2006
    ..We conclude that a gene expression-based optical imaging system can accurately detect and quantify MAPK activity in live animals...
  52. ncbi request reprint Noninvasive monitoring of target gene expression by imaging reporter gene expression in living animals using improved bicistronic vectors
    Yanling Wang
    The Crump Institute for Molecular Imaging, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Nucl Med 46:667-74. 2005
    ....
  53. ncbi request reprint Optical bioluminescence and positron emission tomography imaging of a novel fusion reporter gene in tumor xenografts of living mice
    Pritha Ray
    Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Gefen School of Medicine, University of California, Los Angeles, California 90095, USA
    Cancer Res 63:1160-5. 2003
    ..Using a single fusion reporter (PET/optical) gene should accelerate the validation of reporter gene approaches developed in cell culture for translation into preclinical and clinical models...
  54. doi request reprint A generalizable strategy for imaging pre-mRNA levels in living subjects using spliceosome-mediated RNA trans-splicing
    Zachary F Walls
    Department of Molecular and Medical Pharmacology, Geffen School of Medicine at UCLA, Los Angeles, California, USA
    J Nucl Med 49:1146-54. 2008
    ..Presented here is a class of generalizable probes that can image pre-mRNA in a sequence-specific manner, using signal amplification and a facile method of delivery...
  55. ncbi request reprint Noninvasive, repetitive, quantitative measurement of gene expression from a bicistronic message by positron emission tomography, following gene transfer with adenovirus
    Qianwa Liang
    The Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, California 90095, USA
    Mol Ther 6:73-82. 2002
    ....
  56. ncbi request reprint Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output
    Andreas Markus Loening
    The Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Geffen School of Medicine at UCLA, Los Angeles, CA, USA
    Protein Eng Des Sel 19:391-400. 2006
    ....
  57. ncbi request reprint Quantitation of cell number by a positron emission tomography reporter gene strategy
    Helen Su
    Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
    Mol Imaging Biol 6:139-48. 2004
    ..In this study, we determined the correlation of PET signal to cell number, and characterized the cellular limit of detection for PET imaging...
  58. ncbi request reprint Monitoring the antitumor response of naive and memory CD8 T cells in RAG1-/- mice by positron-emission tomography
    Helen Su
    Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, 90095, USA
    J Immunol 176:4459-67. 2006
    ..Positron-emission tomography-based immunologic imaging is a noninvasive modality providing unique and meaningful information on the dynamics of the antitumor CTL response...
  59. ncbi request reprint Novel bidirectional vector strategy for amplification of therapeutic and reporter gene expression
    Sunetra Ray
    Crump Institute for Molecular Imaging, and Department of Molecular and Medical Pharmacology, UCLA School of Medicine, Los Angeles, CA 90095, USA
    Hum Gene Ther 15:681-90. 2004
    ..This validated approach should prove useful for the development of novel gene therapy vectors, as well as for transgenic models, allowing noninvasive imaging for indirect monitoring and amplification of target gene expression...
  60. ncbi request reprint Molecular imaging in living subjects: seeing fundamental biological processes in a new light
    Tarik F Massoud
    The Crump Institute for Molecular Imaging, David Geffen School of Medicine at University of California at Los Angeles, Los Angeles, California 90095, USA
    Genes Dev 17:545-80. 2003
  61. ncbi request reprint Spontaneous and controllable activation of suicide gene expression driven by the stress-inducible grp78 promoter resulting in eradication of sizable human tumors
    Dezheng Dong
    Department of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, 1441 Eastlake Avenue, Los Angeles, CA 90089 9176, USA
    Hum Gene Ther 15:553-61. 2004
    ..Thus, transcriptional control through the use of the Grp78 promoter offers multiple novel approaches for human cancer gene therapy...
  62. pmc Visualization of a primary anti-tumor immune response by positron emission tomography
    Chengyi J Shu
    Department of Microbiology, Immunology, and Molecular Genetics, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095, USA
    Proc Natl Acad Sci U S A 102:17412-7. 2005
    ..The method we describe can be used to kinetically measure the induction and therapeutic modulations of cell-mediated immune responses...
  63. pmc 18F-FDG uptake in lung, breast, and colon cancers: molecular biology correlates and disease characterization
    Hossein Jadvar
    Department of Radiology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA
    J Nucl Med 50:1820-7. 2009
    ....
  64. ncbi request reprint BRET-based method for detection of specific RNA species
    Zachary F Walls
    Department of Molecular and Medical Pharmacology, Geffen School of Medicine at UCLA, Los Angeles, California, USA
    Bioconjug Chem 19:178-84. 2008
    ..11 x 10 (-6), ANOVA, multiple range test). This assay represents a possibility for a less technically demanding, streamlined alternative to canonical RNA assays...
  65. ncbi request reprint Gene expression tomography
    Vanessa M Brown
    Department of Molecular and Medical Pharmacology, University of California, Los Angeles 90095, USA
    Physiol Genomics 8:159-67. 2002
    ..A Monte-Carlo analysis confirmed the good quality of the GET image reconstruction. By speeding acquisition of gene expression patterns, GET may help improve our understanding of the genomics of the brain in both health and disease...
  66. ncbi request reprint Positron emission tomography imaging analysis of G2A as a negative modifier of lymphoid leukemogenesis initiated by the BCR-ABL oncogene
    Lu Q Le
    Department of Microbiology, Immunology, and Molecular Genetics, Los Angeles, CA 90095, USA
    Cancer Cell 1:381-91. 2002
    ..PET can be successfully applied to the temporal and spatial analysis of Bcr-Abl driven leukemic progression and should have utility for the study of other leukemias and lymphomas...
  67. ncbi request reprint Noninvasive indirect imaging of vascular endothelial growth factor gene expression using bioluminescence imaging in living transgenic mice
    Yanling Wang
    The Crump Institute for Molecular Imaging, Los Angeles, California, USA
    Physiol Genomics 24:173-80. 2006
    ....
  68. ncbi request reprint The impact of PET on the management of lung cancer: the referring physician's perspective
    Marc A Seltzer
    Department of Molecular and Medical Pharmacology, Ahmanson Biological Imaging Clinic Nuclear Medicine, UCLA School of Medicine, Los Angeles, California 90095 6948, USA
    J Nucl Med 43:752-6. 2002
    ..The objective of this study was to determine referring physicians' perspectives on the impact of (18)F-FDG PET on staging and management of lung cancer...
  69. ncbi request reprint Molecular imaging applications for immunology
    Isabel Junie Hildebrandt
    Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA
    Clin Immunol 111:210-24. 2004
    ..In this paper, we will review the current field of molecular imaging assays (especially those utilizing PET and BIm modalities) and examine how they might impact animal models and human disease in the field of clinical immunology...
  70. doi request reprint Perspectives of molecular imaging and radioimmunotherapy in lymphoma
    Andrei Iagaru
    Department of Radiology, Division of Nuclear Medicine, Stanford University Medical Center, 300 Pasteur Drive, Room H 0101, Stanford, CA 94305 5427, USA
    Radiol Clin North Am 46:243-52, viii. 2008
    ..Finally, we discuss advances in molecular imaging that may herald the next generation of PET radiotracers after 18F FDG...
  71. ncbi request reprint Image-guided cardiac cell delivery using high-resolution small-animal ultrasound
    Martin Rodriguez-Porcel
    Department of Internal Medicine, Divisions of Cardiovascular Diseases, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Mol Ther 12:1142-7. 2005
    ..We are confident that the use of these technologies will play a significant role in the future of gene and cell therapy...
  72. doi request reprint Molecualr imaging of cancer: from molecules to humans. Introduction
    Sanjiv Sam Gambhir
    Department of Radiology, Stanford University School of Medicine, Stanford, California 94305 5427, USA
    J Nucl Med 49:1S-4S. 2008
  73. doi request reprint Small-animal PET imaging of human epidermal growth factor receptor type 2 expression with site-specific 18F-labeled protein scaffold molecules
    Zhen Cheng
    Molecular Imaging Program at Stanford, Department of Radiology, Bio X Program, Stanford University, Stanford, California 94305, USA
    J Nucl Med 49:804-13. 2008
    ....
  74. pmc Reporter gene imaging following percutaneous delivery in swine moving toward clinical applications
    Martin Rodriguez-Porcel
    J Am Coll Cardiol 51:595-7. 2008
  75. ncbi request reprint Multimodality imaging of T-cell hybridoma trafficking in collagen-induced arthritic mice: image-based estimation of the number of cells accumulating in mouse paws
    Shahriar S Yaghoubi
    Stanford University, Department of Radiology, Bio X, Molecular Imaging Program, Stanford, California 94305 5427, USA
    J Biomed Opt 12:064025. 2007
    ..The procedures described in this study can be used to derive equations for cells expressing other bioluminescent RGs and in other animal models...
  76. pmc Near-infrared fluorescent deoxyglucose analogue for tumor optical imaging in cell culture and living mice
    Zhen Cheng
    Molecular Imaging Program at Stanford MIPS, Department of Radiology, Stanford University, Stanford, California, 94305 5344, USA
    Bioconjug Chem 17:662-9. 2006
    ..To develop NIR glucose analogues with the ability to target GLUTs/hexokinase, it is highly important to select NIR dyes with a reasonable molecular size...
  77. ncbi request reprint 123I MIBG mapping with intraoperative gamma probe for recurrent neuroblastoma
    Andrei Iagaru
    Department of Radiology, Division of Nuclear Medicine, Stanford University Medical Center, Stanford, CA 94305 5427, USA
    Mol Imaging Biol 10:19-23. 2008
    ..Intraoperative gamma probe mapping of the liver identified areas with signal above the background, but these were prove to be hemosiderin deposits on histo-pathology examination...
  78. ncbi request reprint Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia
    Ivana Cecic
    Department of Radiation Oncology and Molecular Imaging Program, Stanford, Stanford University School of Medicine, Stanford, CA 94305 5847, USA
    Mol Imaging 6:219-28. 2007
    ..These results demonstrate that combining beta-galactosidase with the DDAOG optical probe may be a robust reporter system for the in vivo study of tumor hypoxia...
  79. ncbi request reprint Positron emission tomography in diagnosis and management of invasive breast cancer: current status and future perspectives
    Dafang Wu
    Department of Radiology, Wayne State University, PET Center, Children s Hospital of Michigan, Detroit, MI, USA
    Clin Breast Cancer 4:S55-63. 2003
    ..role of FDG-PET in breast cancer is increasing and evolving, and this metabolic imaging modality, in conjunction with newer tracers and other anatomic imaging methods, should improve diagnosis and management of patients with breast cancer..
  80. ncbi request reprint Standardized uptake value atlas: characterization of physiological 2-deoxy-2-[18F]fluoro-D-glucose uptake in normal tissues
    Yingbing Wang
    Molecular Imaging Program at Stanford MIPS, Department of Radiology, Division of Nuclear Medicine, Stanford University, Stanford, CA 94305 5427, USA
    Mol Imaging Biol 9:83-90. 2007
    ..The purpose of this study was to map the distribution of 2-deoxy-2-[18F]fluoro-D-glucose (FDG) uptake in organs of patients with no known abnormalities in those tissues...
  81. ncbi request reprint Molecular imaging can accelerate anti-angiogenic drug development and testing
    Andrei Iagaru
    Stanford University School of Medicine, Stanford, CA, USA
    Nat Clin Pract Oncol 4:556-7. 2007