Fakhri Saida

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi First structural investigation of the restriction ribonuclease RegB: NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau Cedex, France
    Protein Expr Purif 34:158-65. 2004
  2. ncbi Expression of highly toxic genes in E. coli: special strategies and genetic tools
    F Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla CA 92093 0634, USA
    Curr Protein Pept Sci 7:47-56. 2006
  3. ncbi Statistical analysis of 15 dimensions in the crystallization space for protein-DNA complexes
    Fakhri Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Protein Pept Lett 13:929-39. 2006
  4. ncbi RNA recognition and cleavage by sequence-specific endoribonucleases
    Fakhri Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla CA 92093 0634, USA
    Protein Pept Lett 14:103-11. 2007
  5. ncbi Structural characterization of the interaction between TFIIIB components Bdp1 and Brf1
    Fakhri Saida
    Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Biochemistry 47:13197-206. 2008
  6. ncbi New system for positive selection of recombinant plasmids and dual expression in yeast and bacteria based on the restriction ribonuclease RegB
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128, Palaiseau Cedex, France
    Biotechnol Prog 19:727-33. 2003
  7. ncbi The phage T4 restriction endoribonuclease RegB: a cyclizing enzyme that requires two histidines to be fully active
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France
    Nucleic Acids Res 31:2751-8. 2003
  8. ncbi Structural and functional studies of RegB, a new member of a family of sequence-specific ribonucleases involved in mRNA inactivation on the ribosome
    Benoit Odaert
    ICSN RMN, Institut de Chimie des Substances Naturelles, CNRS de Gif sur Yvette, 91190 Gif sur Yvette et Ecole Polytechnique, 91128 Palaiseau, France
    J Biol Chem 282:2019-28. 2007
  9. ncbi Overview on the expression of toxic gene products in Escherichia coli
    Fakhri Saida
    University of California San Diego, La Jolla, California, USA
    Curr Protoc Protein Sci . 2007

Collaborators

Detail Information

Publications9

  1. ncbi First structural investigation of the restriction ribonuclease RegB: NMR spectroscopic conditions, 13C/15N double-isotopic labelling and two-dimensional heteronuclear spectra
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau Cedex, France
    Protein Expr Purif 34:158-65. 2004
    ..Finally, we present the first (1)H/(15)N and (1)H/(13)C two-dimensional NMR spectra of RegB H48A. These spectra show that the protein is folded and that the full structural analysis of RegB by NMR is feasible...
  2. ncbi Expression of highly toxic genes in E. coli: special strategies and genetic tools
    F Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla CA 92093 0634, USA
    Curr Protein Pept Sci 7:47-56. 2006
    ..Expression then occurs efficiently before cells die. Because they do not target a particular toxic effect, these strategies and tools can be used to express a wide variety of highly toxic genes...
  3. ncbi Statistical analysis of 15 dimensions in the crystallization space for protein-DNA complexes
    Fakhri Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0634, USA
    Protein Pept Lett 13:929-39. 2006
    ..These restrictions could be very helpful in the design of sparse-matrix crystallization screens that target exclusively protein-DNA complexes...
  4. ncbi RNA recognition and cleavage by sequence-specific endoribonucleases
    Fakhri Saida
    Center for Molecular Genetics, University of California San Diego, 9500 Gilman Drive, La Jolla CA 92093 0634, USA
    Protein Pept Lett 14:103-11. 2007
    ....
  5. ncbi Structural characterization of the interaction between TFIIIB components Bdp1 and Brf1
    Fakhri Saida
    Center for Molecular Genetics, University of California at San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA
    Biochemistry 47:13197-206. 2008
    ..The main Bdp1 anchorage site is provided by a small set of residues belonging to a Brf1 segment of residues 470-495...
  6. ncbi New system for positive selection of recombinant plasmids and dual expression in yeast and bacteria based on the restriction ribonuclease RegB
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128, Palaiseau Cedex, France
    Biotechnol Prog 19:727-33. 2003
    ..coli and in the yeast Saccharomyces cerevisiae from a single plasmid. Hence, this vector may be a useful time-saving tool for one-step cloning and versatile protein expression in bacteria and yeast...
  7. ncbi The phage T4 restriction endoribonuclease RegB: a cyclizing enzyme that requires two histidines to be fully active
    Fakhri Saida
    Laboratoire ICSN RMN, Institut de Chimie des Substances Naturelles, Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France
    Nucleic Acids Res 31:2751-8. 2003
    ..Altogether, our results define RegB as a new cyclizing RNase and present His48 and His68 as potent catalytic residues. The effect of the in vivo selected R52L mutation is also described and discussed...
  8. ncbi Structural and functional studies of RegB, a new member of a family of sequence-specific ribonucleases involved in mRNA inactivation on the ribosome
    Benoit Odaert
    ICSN RMN, Institut de Chimie des Substances Naturelles, CNRS de Gif sur Yvette, 91190 Gif sur Yvette et Ecole Polytechnique, 91128 Palaiseau, France
    J Biol Chem 282:2019-28. 2007
    ..Although these ribonucleases have different catalytic sites, we propose that RegB is a new member of the RelE/YoeB structural and functional family of ribonucleases specialized in mRNA inactivation within the ribosome...
  9. ncbi Overview on the expression of toxic gene products in Escherichia coli
    Fakhri Saida
    University of California San Diego, La Jolla, California, USA
    Curr Protoc Protein Sci . 2007
    ..Therefore, modified expression vectors, modified E. coli strains, and appropriate cultivation protocols are needed. This overview discusses several special strategies successfully used to express toxic genes in E. coli...