M Sahin-Toth

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi The pathobiochemistry of hereditary pancreatitis: studies on recombinant human cationic trypsinogen
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Calif, USA
    Pancreatology 1:461-5. 2001
  2. ncbi High-affinity Ca(2+) binding inhibits autoactivation of rat trypsinogen
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochem Biophys Res Commun 275:668-71. 2000
  3. ncbi Human cationic trypsinogen. Role of Asn-21 in zymogen activation and implications in hereditary pancreatitis
    M Sahin-Toth
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    J Biol Chem 275:22750-5. 2000
  4. ncbi Trypsinogen stabilization by mutation Arg117-->His: a unifying pathomechanism for hereditary pancreatitis?
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California, 90095 1662, USA
    Biochem Biophys Res Commun 264:505-8. 1999
  5. ncbi Hereditary pancreatitis-associated mutation asn(21) --> ile stabilizes rat trypsinogen in vitro
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    J Biol Chem 274:29699-704. 1999
  6. ncbi Cloning, sequencing, and expression of cscA invertase from Escherichia coli B-62
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles 90095 1662, USA
    Can J Microbiol 45:418-22. 1999
  7. ncbi Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins
    S Frillingos
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90024
    FASEB J 12:1281-99. 1998
  8. ncbi Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles 90095 1662, USA
    Biochemistry 38:813-9. 1999
  9. pmc The role of transmembrane domain III in the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024 1662, USA
    Protein Sci 3:2302-10. 1994
  10. pmc Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024 1662
    Proc Natl Acad Sci U S A 91:5421-5. 1994

Detail Information

Publications15

  1. ncbi The pathobiochemistry of hereditary pancreatitis: studies on recombinant human cationic trypsinogen
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Calif, USA
    Pancreatology 1:461-5. 2001
    ..This study attempts to identify the biochemical alterations in human cationic trypsinogen and trypsin caused by the hereditary pancreatitis-associated mutations Arg117-->His and Asn21-->Ile...
  2. ncbi High-affinity Ca(2+) binding inhibits autoactivation of rat trypsinogen
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochem Biophys Res Commun 275:668-71. 2000
    ..7-2.4 mM). The results indicate that rat Tg is subject to dual regulation by Ca(2+), allowing zymogen stabilization in a low-Ca(2+) environment and efficient activation in a high-Ca(2+) milieu...
  3. ncbi Human cationic trypsinogen. Role of Asn-21 in zymogen activation and implications in hereditary pancreatitis
    M Sahin-Toth
    Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    J Biol Chem 275:22750-5. 2000
    ..In the same context, faster autoactivation and increased trypsin stability caused by the Asn-21 --> Thr mutation in human Tg-1 might provide a rationale for the evolutionary divergence from Thr-21 found in other mammalian trypsinogens...
  4. ncbi Trypsinogen stabilization by mutation Arg117-->His: a unifying pathomechanism for hereditary pancreatitis?
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California, 90095 1662, USA
    Biochem Biophys Res Commun 264:505-8. 1999
    ....
  5. ncbi Hereditary pancreatitis-associated mutation asn(21) --> ile stabilizes rat trypsinogen in vitro
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    J Biol Chem 274:29699-704. 1999
    ....
  6. ncbi Cloning, sequencing, and expression of cscA invertase from Escherichia coli B-62
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles 90095 1662, USA
    Can J Microbiol 45:418-22. 1999
    ..coli EC3132. Functional characterization indicates that high-level expression and limited periplasmic release of invertase is responsible for the sucrose-fermenting capacity of transformed E. coli K-12 strains carrying cscA...
  7. ncbi Cys-scanning mutagenesis: a novel approach to structure function relationships in polytopic membrane proteins
    S Frillingos
    Howard Hughes Medical Institute, Departments of Physiology and Microbiology and Molecular Genetics, Molecular Biology Institute, University of California Los Angeles, Los Angeles, California 90024
    FASEB J 12:1281-99. 1998
    ..structure-function relationships in polytopic membrane proteins...
  8. ncbi Characterization of Glu126 and Arg144, two residues that are indispensable for substrate binding in the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles 90095 1662, USA
    Biochemistry 38:813-9. 1999
    ..The results extend the conclusion that a carboxylate at position 126 and a guanidinium group at position 144 are irreplaceable for substrate binding and support the idea that Arg144 plays a major role in substrate specificity...
  9. pmc The role of transmembrane domain III in the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024 1662, USA
    Protein Sci 3:2302-10. 1994
    ..The observations demonstrate that although no residue per se appears to be essential, structural properties of helix III are important for active lactose transport...
  10. pmc Properties of permease dimer, a fusion protein containing two lactose permease molecules from Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024 1662
    Proc Natl Acad Sci U S A 91:5421-5. 1994
    ..The approach is consistent with the contention that the functional unit of lactose permease is a monomer...
  11. pmc Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles 90024 1574
    Proc Natl Acad Sci U S A 89:10547-51. 1992
    ..In any event, the findings suggest that putative transmembrane helix VII lies next to helices X and XI in the tertiary structure of lactose permease...
  12. ncbi The kamikaze approach to membrane transport
    H R Kaback
    Howard Hughes Medical Institute, Department of Physiology, University of California, Los Angeles, California 90095 1662, USA
    Nat Rev Mol Cell Biol 2:610-20. 2001
    ..Novel approaches that have been developed and applied to one membrane transport protein, the lactose permease from Escherichia coli, are now being used to study various other membrane proteins...
  13. pmc Arg-302 facilitates deprotonation of Glu-325 in the transport mechanism of the lactose permease from Escherichiacoli
    M Sahin-Toth
    Howard Hughes Medical Institute, Department of Physiology, Molecular Biology Institute, University of California, Los Angeles, CA 90095-1662, USA
    Proc Natl Acad Sci U S A 98:6068-73. 2001
    ..These observations lend strong support for the argument that Arg-302 interacts with Glu-325 to facilitate deprotonation of the carboxylic acid during turnover...
  14. ncbi [Significance of trypsinogen gene mutations in the etiology of hereditary pancreatitis]
    M Sahin-Toth
    Department of Physiology, University of California Los Angeles, Los Angeles, California, USA
    Orv Hetil 142:603-6. 2001
    ..This notion is strongly supported by the clear correlation between the autoactivation rates of mutant trypsinogens and the severity of clinical symptoms...
  15. ncbi The C-4 hydroxyl group of galactopyranosides is the major determinant for ligand recognition by the lactose permease of Escherichia coli
    M Sahin-Toth
    Howard Hughes Medical Institute, University of California Los Angeles, Los Angeles, California 90095 1662, USA
    Biochemistry 40:13015-9. 2001
    ..Moreover, the C-4 hydroxyl is identified as the major determinant of ligand binding, suggesting that sugar recognition in lactose permease may have evolved to discriminate primarily between gluco- and galactopyranosides...