F Sachs

Summary

Affiliation: University at Buffalo
Country: USA

Publications

  1. pmc Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytes
    Shinghua Ding
    Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, NY 14214, USA
    BMC Neurosci 3:17. 2002
  2. pmc Stretch-activated ion channels: what are they?
    Frederick Sachs
    State University of New York, Buffalo, New York, USA
    Physiology (Bethesda) 25:50-6. 2010
  3. pmc Practical limits on the maximal speed of solution exchange for patch clamp experiments
    F Sachs
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 77:682-90. 1999
  4. pmc Hidden Markov modeling for single channel kinetics with filtering and correlated noise
    F Qin
    Department of Physiology and Biophysical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 79:1928-44. 2000
  5. pmc Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed events
    F Qin
    Department of Biophysical Sciences, State University of New York at Buffalo, New York 14214, USA
    Biophys J 70:264-80. 1996
  6. ncbi request reprint Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers
    Thomas M Suchyna
    Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, New York 14214, USA
    Nature 430:235-40. 2004
  7. pmc Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels
    T M Suchyna
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Gen Physiol 115:583-98. 2000
  8. ncbi request reprint cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of mechanosensitive channels
    Kimberly Laskie Ostrow
    Center for Single Molecule Biophysics, SUNY at Buffalo, 320 Cary Hall, Buffalo, NY 14214, USA
    Toxicon 42:263-74. 2003
  9. pmc Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology
    Charles L Bowman
    Center for Single Molecule Biophysics and the Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, NY 14214, USA
    Toxicon 49:249-70. 2007
  10. ncbi request reprint Mechanosensitive ion channels as drug targets
    Philip A Gottlieb
    Center for Molecular Biophysics, 301 Cary Hall, SUNY, Buffalo, NY 14214, USA
    Curr Drug Targets CNS Neurol Disord 3:287-95. 2004

Research Grants

  1. AFM Of Voltage And Mechanically Gated Channels
    Frederick Sachs; Fiscal Year: 2004
  2. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2006
  3. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2007
  4. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2007
  5. Cell mechanics and mechanical transduction by ion channels
    Frederick Sachs; Fiscal Year: 2009
  6. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 1999
  7. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2003
  8. Cell mechanics and mechanical transduction by ion channels
    Frederick Sachs; Fiscal Year: 2010

Collaborators

Detail Information

Publications43

  1. pmc Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytes
    Shinghua Ding
    Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, NY 14214, USA
    BMC Neurosci 3:17. 2002
    ..P2X2 receptor is an ATP-activated ion channel which is widely expressed in the nervous system, and mediates synaptic transmission...
  2. pmc Stretch-activated ion channels: what are they?
    Frederick Sachs
    State University of New York, Buffalo, New York, USA
    Physiology (Bethesda) 25:50-6. 2010
    ..Understanding MSC function requires under standing the stimulus...
  3. pmc Practical limits on the maximal speed of solution exchange for patch clamp experiments
    F Sachs
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 77:682-90. 1999
    ..Increasing the perfusate viscosity improves speed by slowing dissipation of the concentration gradients. A flow switcher can also be used for bipolar temperature jumps with a rise time of approximately 100 microseconds...
  4. pmc Hidden Markov modeling for single channel kinetics with filtering and correlated noise
    F Qin
    Department of Physiology and Biophysical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 79:1928-44. 2000
    ..Examples are presented that demonstrate the applicability and effectiveness of the algorithm. Practical issues such as the selection of appropriate noise AR orders are also discussed through examples...
  5. pmc Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed events
    F Qin
    Department of Biophysical Sciences, State University of New York at Buffalo, New York 14214, USA
    Biophys J 70:264-80. 1996
    ..It is very efficient and robust; rate constants for a multistate model can often be extracted in a processing time of approximately 1 min, largely independent of the starting values...
  6. ncbi request reprint Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomers
    Thomas M Suchyna
    Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, New York 14214, USA
    Nature 430:235-40. 2004
    ..The surprising efficacy of the d form of GsMTx4 peptide has important therapeutic implications, because d peptides are not hydrolysed by endogenous proteases and may be administered orally...
  7. pmc Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels
    T M Suchyna
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    J Gen Physiol 115:583-98. 2000
    ..This is the first report of a peptide toxin that specifically blocks stretch-activated currents. The toxin affect on swelling-activated whole-cell currents implicates SACs in volume regulation...
  8. ncbi request reprint cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of mechanosensitive channels
    Kimberly Laskie Ostrow
    Center for Single Molecule Biophysics, SUNY at Buffalo, 320 Cary Hall, Buffalo, NY 14214, USA
    Toxicon 42:263-74. 2003
    ..The retention time in HPLC analysis and the CD spectrum was similar to wild type. Both the synthetic and cloned peptides were active in the patch clamp assay...
  9. pmc Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology
    Charles L Bowman
    Center for Single Molecule Biophysics and the Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, NY 14214, USA
    Toxicon 49:249-70. 2007
    ..In this review, we summarize the current knowledge of GsMTx-4, and discuss the inevitable crosstalk between the MSC behavior and the mechanical properties of the cell cortex...
  10. ncbi request reprint Mechanosensitive ion channels as drug targets
    Philip A Gottlieb
    Center for Molecular Biophysics, 301 Cary Hall, SUNY, Buffalo, NY 14214, USA
    Curr Drug Targets CNS Neurol Disord 3:287-95. 2004
    ..By itself, GsMTx4 can serve as a lead compound or as a potential drug. Its availability opens clinical horizons in the diagnosis and treatment of pathologies including cardiac arrhythmia, muscular dystrophy and glioma...
  11. ncbi request reprint Dynamic regulation of mechanosensitive channels: capacitance used to monitor patch tension in real time
    Thomas M Suchyna
    Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, NY 14214, USA
    Phys Biol 1:1-18. 2004
    ..Surprisingly, multivalent ions such as Gd(+3) and Ca(+2) that bind to the lipids and affect channel gating, do not affect the strain-induced increase in membrane capacitance; contrary to expectations, membrane elasticity is unchanged...
  12. pmc Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubes
    Thomas M Suchyna
    Department of Physiology and Biophysics, Center for Single Molecule Biophysics, State University New York SUNY at Buffalo, Buffalo, NY 14214, USA
    J Physiol 581:369-87. 2007
    ..Spontaneous Ca2+ transients in mdx mouse cells are sensitive to depolarization and are inhibited by the specific MSC inhibitor GsMTx4, in both the D and L forms...
  13. ncbi request reprint Microfluidic actuation using electrochemically generated bubbles
    Susan Z Hua
    Center for Bio MEMS, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14260, USA
    Anal Chem 74:6392-6. 2002
    ..Using such bubble valves, an eight-way multiplexer was fabricated and tested...
  14. pmc Neurite outgrowth from PC12 cells is enhanced by an inhibitor of mechanical channels
    Philip A Gottlieb
    Physiology and Biophysics, 301 Cary Hall, SUNY at Buffalo, Buffalo, NY 14214, USA
    Neurosci Lett 481:115-9. 2010
    ..The inhibition of mechanosensitive channels by GsMTx4 may be a useful approach to accelerate regeneration of neurons in neurodegenerative diseases and spinal cord injury...
  15. ncbi request reprint High-speed pressure clamp
    Stephen R Besch
    Center for Single Molecule Biophysics, 320 Cary Hall, SUNY at Buffalo, Buffalo, NY 14214, USA
    Pflugers Arch 445:161-6. 2002
    ..5 ms for a 100-mmHg step, and the closed-loop settling time is 500-600 micros. Valve actuation latency is 120 micros. The system performance is illustrated for mechanically induced changes in patch capacitance...
  16. pmc Biophysics and structure of the patch and the gigaseal
    Thomas M Suchyna
    State University of New York at Buffalo, Department of Physiology and Biophysics, Buffalo, New York 14214, USA
    Biophys J 97:738-47. 2009
    ..Voltage-dependent creep seems to arise from electroosmosis in the seal. Neutralization of negative charges on the seal membrane with low pH decreased the creep rate and reversed the direction of creep at positive pipette potentials...
  17. pmc A mechanosensitive ion channel regulating cell volume
    Susan Z Hua
    Department of Physiology and Biophysics, SUNY, Buffalo, NY 14214, USA
    Am J Physiol Cell Physiol 298:C1424-30. 2010
    ..The NRK cells form a stable platform from which to screen drugs that affect volume regulation via mechanosensory channels and as a sensitive system to clone the channel...
  18. pmc Dynamic effects of Hg2+-induced changes in cell volume
    Jinseok Heo
    Department of Mechanical and Aerospace Engineering, SUNY Buffalo, 340 Jarvis Hall, Buffalo, NY 14260, USA
    Cell Biochem Biophys 51:21-32. 2008
    ....
  19. ncbi request reprint Mechanosensation and endothelin in astrocytes--hypothetical roles in CNS pathophysiology
    Lyle W Ostrow
    Department of Physiology and Biophysics, S U N Y at Buffalo, School of Medicine and Biomedical Sciences, Buffalo, NY 14214, USA
    Brain Res Brain Res Rev 48:488-508. 2005
    ....
  20. ncbi request reprint Volume cytometry: microfluidic sensor for high-throughput screening in real time
    Daniel A Ateya
    Bio MEMS and Bio Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY Buffalo, Buffalo, New York 14260, USA
    Anal Chem 77:1290-4. 2005
    ..The prototype chip made in silicon is inexpensive, reusable, and runs on low-voltage electrical power. The technology can be readily transferred to large arrays in plastic...
  21. ncbi request reprint Connexins are mechanosensitive
    Li Bao
    Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33136, USA
    Am J Physiol Cell Physiol 287:C1389-95. 2004
    ..Physiologically, Cx46 hemichannels may assist accommodation of the ocular lens by providing a transient path for volume flow as the lens changes shape...
  22. ncbi request reprint Flexoelectricity and elasticity of asymmetric biomembranes
    Alexander G Petrov
    Department of Physiology and Biophysics, SUNY Buffalo, 320 Cary Hall, Buffalo, New York 14214, USA
    Phys Rev E Stat Nonlin Soft Matter Phys 65:021905. 2002
    ..The results are discussed with respect to data from atomic force microscopy studies of electromotility in biomembranes...
  23. pmc Maximum likelihood estimation of ion channel kinetics from macroscopic currents
    Lorin S Milescu
    Department of Physiology and Biophysics, State University of New York, Buffalo, New York, USA
    Biophys J 88:2494-515. 2005
    ..Given appropriate stimulation protocols, our method chose a reasonable model size and topology...
  24. doi request reprint Microfluidic chip to produce temperature jumps for electrophysiology
    Thomas Pennell
    Department of Mechanical and Aerospace Engineering, State University of New York Buffalo, Buffalo, New York 14260, USA
    Anal Chem 80:2447-51. 2008
    ..The heat-stimulated currents were observed using whole-cell and cell-attached patch configurations. The results demonstrated that the chip can provide rapid temperature jumps at the resolution of single-ion channels...
  25. ncbi request reprint Revisiting TRPC1 and TRPC6 mechanosensitivity
    Philip Gottlieb
    Single Molecule Biophysics, SUNY, 301 Cary Hall, Buffalo, NY 14214, USA
    Pflugers Arch 455:1097-103. 2008
    ..In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic...
  26. pmc A direct optimization approach to hidden Markov modeling for single channel kinetics
    F Qin
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Biophys J 79:1915-27. 2000
    ....
  27. ncbi request reprint Voltage-induced membrane movement
    P C Zhang
    HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
    Nature 413:428-32. 2001
    ..As salicylate blocks electromotility in cochlear outer hair cells at the same concentration, the role of prestin as a motor protein may need to be reassessed...
  28. pmc A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situ
    Fanjie Meng
    Center for Single Molecule Biophysics, Department of Physiology and Biophysics, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY 14214, USA
    FEBS J 275:3072-87. 2008
    ..When collagen-19 was labeled near its middle with stFRET, it expressed well in Caenorhabditis elegans, distributing similarly to hosts labeled with a terminal green fluorescent protein, and the worms behaved normally...
  29. ncbi request reprint Drift-free atomic force microscopy measurements of cell height and mechanical properties
    Chiara Spagnoli
    Department of Physiology and Biophysics, University at Buffalo, State University of New York, 3435 Main Street, 301 Cary Hall, Buffalo, NY 14214, USA
    Rev Sci Instrum 78:036111. 2007
    ..1 Hz. As a proof of concept, we applied the SaM to study the response of rat astrocytes to osmotic stress, observing dimensional and constitutive changes during volume regulation...
  30. pmc Desensitization of mechano-gated K2P channels
    Eric Honore
    Single Molecule Biophysics, 301 Cary Hall, University at Buffalo, State University of New York, Buffalo, NY 14214, USA
    Proc Natl Acad Sci U S A 103:6859-64. 2006
    ..Desensitization and its regulation by chemical messengers is predicted to condition the physiological role of K2P channels...
  31. ncbi request reprint Adapting the Quesant Nomad atomic force microscope for biology and patch-clamp atomic force microscopy
    S Besch
    Huges Center for Single Molecule Biophysics, Physiology and Biophysical Sciences, SUNY at Buffalo, Buffalo, NY 14214, USA
    Cell Biochem Biophys 39:195-210. 2003
    ..The correlation of electrical and mechanical information allows signal averaging and measures sub-Angstrom, sub-millisecond electromotile responses from cells...
  32. pmc Extracting dwell time sequences from processive molecular motor data
    Lorin S Milescu
    Department of Physiology and Biophysics, State University of New York, Buffalo, New York, USA
    Biophys J 91:3135-50. 2006
    ..The kinetic consequences of the extracted dwell sequence can be further analyzed in detail. We show results from analyzing simulated and experimental kinesin and myosin motor data. The algorithm is implemented in the free QuB software...
  33. pmc Maximum likelihood estimation of molecular motor kinetics from staircase dwell-time sequences
    Lorin S Milescu
    Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York, USA
    Biophys J 91:1156-68. 2006
    ..The algorithm was tested with simulated data, and implemented in the free QuB software...
  34. ncbi request reprint Dynamic properties of stretch-activated K+ channels in adult rat atrial myocytes
    Weizhen Niu
    Department of Physiology, Capital University of Medical Sciences, 100054, Beijing, People s Republic of China
    Prog Biophys Mol Biol 82:121-35. 2003
    ..The time dependent behavior of these SACs is generally consistent with whole-cell currents observed in chick and rat ventricular cells, although the net current was outward rather than inward...
  35. pmc Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouse
    Ella W Yeung
    School of Medical Sciences and Institute for Biomedical Research, University of Sydney F13, NSW 2006, Australia
    J Physiol 562:367-80. 2005
    ..This result suggests that blockers of stretch-activated channels may protect against muscle damage in the intact mdx mouse...
  36. ncbi request reprint Solution structure of peptide toxins that block mechanosensitive ion channels
    Robert E Oswald
    Department of Molecular Medicine, Cornell University, C3 167 Veterinary Medical Center, Ithaca, NY 14853, USA
    J Biol Chem 277:34443-50. 2002
    ..The spatial arrangement of charged residues that are unique to GsMTx-4 and GsMTx-2 may underlie the selectivity of these peptides...
  37. pmc Angiotensin II and myosin light-chain phosphorylation contribute to the stretch-induced slow force response in human atrial myocardium
    Jens Kockskamper
    Division of Cardiology, Medical University of Graz, Auenbruggerplatz 15, A 8036 Graz, Austria
    Cardiovasc Res 79:642-51. 2008
    ..The functional effects of stretch on human atrium, however, are poorly understood. Thus, we characterized the stretch-induced force response in human atrium and evaluated the underlying cellular mechanisms...
  38. pmc Is lipid bilayer binding a common property of inhibitor cysteine knot ion-channel blockers?
    Yevgen O Posokhov
    Biophys J 93:L20-2. 2007
    ..The high variability of 5 kcal/mole in observed DeltaG shows that bilayer partitioning is not a universal property of the ICK peptides interacting with ion channels...
  39. ncbi request reprint Ca2+ influx through mechanosensitive channels inhibits neurite outgrowth in opposition to other influx pathways and release from intracellular stores
    Bridget T Jacques-Fricke
    Department of Anatomy, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Neurosci 26:5656-64. 2006
    ....
  40. ncbi request reprint Streptomycin reduces stretch-induced membrane permeability in muscles from mdx mice
    Nicholas P Whitehead
    School of Medical Sciences and Institute for Biomedical Research, University of Sydney F13, Sydney, NSW, Australia
    Neuromuscul Disord 16:845-54. 2006
    ..The results suggest that Ca(2+) entry through SACs activates Ca(2+) -dependent pathways, which are the main cause of the increased membrane permeability in mdx muscle...
  41. ncbi request reprint Up- and down-regulation of the mechano-gated K(2P) channel TREK-1 by PIP (2) and other membrane phospholipids
    Jean Chemin
    Institut de Genomique Fonctionnelle, UPR 2580 CNRS, 141 rue de la Cardonille, 34094, Montpellier Cedex 05, France
    Pflugers Arch 455:97-103. 2007
    ..This is, to our knowledge, the first report of an ion channel dually regulated by membrane phospholipids...
  42. ncbi request reprint Retaining your identity under stress
    Frederick Sachs
    Nat Struct Biol 9:636-7. 2002
  43. pmc The slow force response to stretch in atrial and ventricular myocardium from human heart: functional relevance and subcellular mechanisms
    Jens Kockskamper
    Department of Cardiology, Medical University of Graz, Auenbruggerplatz 15, Graz, Austria
    Prog Biophys Mol Biol 97:250-67. 2008
    ..Increased myofilament Ca2+ sensitivity through MLCK-induced phosphorylation of MLC2 is a novel mechanism contributing to the SFR in both atrium and ventricle...

Research Grants26

  1. AFM Of Voltage And Mechanically Gated Channels
    Frederick Sachs; Fiscal Year: 2004
    ..The local electrical field around S4 will be measured by the electrically generated force as a function of the valence at known positions of S4. ..
  2. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2006
    ..Aim 4. Measure the binding of peptides to lipid membranes using tryptophan fluorescence. Aim 5. Solve the 3D NMR structure of selected peptides. ..
  3. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2007
    ..This knowledge will help pave the way for channel isolation by affinity chromatography and rational drug design. ..
  4. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2007
    ..This knowledge will help pave the way for channel isolation by affinity chromatography and rational drug design. ..
  5. Cell mechanics and mechanical transduction by ion channels
    Frederick Sachs; Fiscal Year: 2009
    ..This proposal analyses how stresses are distributed in molecules, membranes and cells and how that leads to signal transduction by ion channels. ..
  6. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 1999
    ....
  7. MECHANICAL TRANSDUCTION BY CARDIOCYTES
    Frederick Sachs; Fiscal Year: 2003
    ..Using the patch clamp and imaging of the patch we will measure the membrane mechanics of patches and the activation of endogenous and cloned MSCs as a function of the status of the cytoskeleton and extracellular matrix. ..
  8. Cell mechanics and mechanical transduction by ion channels
    Frederick Sachs; Fiscal Year: 2010
    ..This proposal analyses how stresses are distributed in molecules, membranes and cells and how that leads to signal transduction by ion channels. ..