Research Topics
| F SachsSummaryAffiliation: University at Buffalo Country: USA Publications
Research Grants
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Detail Information
Publications
Evidence for non-independent gating of P2X2 receptors expressed in Xenopus oocytesShinghua Ding
Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, NY 14214, USA
BMC Neurosci 3:17. 2002..P2X2 receptor is an ATP-activated ion channel which is widely expressed in the nervous system, and mediates synaptic transmission...- Stretch-activated ion channels: what are they?Frederick Sachs
State University of New York, Buffalo, New York, USA
Physiology (Bethesda) 25:50-6. 2010..Understanding MSC function requires under standing the stimulus... - Practical limits on the maximal speed of solution exchange for patch clamp experimentsF Sachs
Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
Biophys J 77:682-90. 1999..Increasing the perfusate viscosity improves speed by slowing dissipation of the concentration gradients. A flow switcher can also be used for bipolar temperature jumps with a rise time of approximately 100 microseconds... - Hidden Markov modeling for single channel kinetics with filtering and correlated noiseF Qin
Department of Physiology and Biophysical Sciences, State University of New York at Buffalo, Buffalo, New York 14214, USA
Biophys J 79:1928-44. 2000..Examples are presented that demonstrate the applicability and effectiveness of the algorithm. Practical issues such as the selection of appropriate noise AR orders are also discussed through examples... - Estimating single-channel kinetic parameters from idealized patch-clamp data containing missed eventsF Qin
Department of Biophysical Sciences, State University of New York at Buffalo, New York 14214, USA
Biophys J 70:264-80. 1996..It is very efficient and robust; rate constants for a multistate model can often be extracted in a processing time of approximately 1 min, largely independent of the starting values... 
Bilayer-dependent inhibition of mechanosensitive channels by neuroactive peptide enantiomersThomas M Suchyna
Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, New York 14214, USA
Nature 430:235-40. 2004..The surprising efficacy of the d form of GsMTx4 peptide has important therapeutic implications, because d peptides are not hydrolysed by endogenous proteases and may be administered orally...- Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channelsT M Suchyna
Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
J Gen Physiol 115:583-98. 2000..This is the first report of a peptide toxin that specifically blocks stretch-activated currents. The toxin affect on swelling-activated whole-cell currents implicates SACs in volume regulation... - cDNA sequence and in vitro folding of GsMTx4, a specific peptide inhibitor of mechanosensitive channelsKimberly Laskie Ostrow
Center for Single Molecule Biophysics, SUNY at Buffalo, 320 Cary Hall, Buffalo, NY 14214, USA
Toxicon 42:263-74. 2003..The retention time in HPLC analysis and the CD spectrum was similar to wild type. Both the synthetic and cloned peptides were active in the patch clamp assay... - Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacologyCharles L Bowman
Center for Single Molecule Biophysics and the Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, NY 14214, USA
Toxicon 49:249-70. 2007..In this review, we summarize the current knowledge of GsMTx-4, and discuss the inevitable crosstalk between the MSC behavior and the mechanical properties of the cell cortex... - Mechanosensitive ion channels as drug targetsPhilip A Gottlieb
Center for Molecular Biophysics, 301 Cary Hall, SUNY, Buffalo, NY 14214, USA
Curr Drug Targets CNS Neurol Disord 3:287-95. 2004..By itself, GsMTx4 can serve as a lead compound or as a potential drug. Its availability opens clinical horizons in the diagnosis and treatment of pathologies including cardiac arrhythmia, muscular dystrophy and glioma... - Dynamic regulation of mechanosensitive channels: capacitance used to monitor patch tension in real timeThomas M Suchyna
Department of Physiology and Biophysics, SUNY at Buffalo, Buffalo, NY 14214, USA
Phys Biol 1:1-18. 2004..Surprisingly, multivalent ions such as Gd(+3) and Ca(+2) that bind to the lipids and affect channel gating, do not affect the strain-induced increase in membrane capacitance; contrary to expectations, membrane elasticity is unchanged... - Mechanosensitive channel properties and membrane mechanics in mouse dystrophic myotubesThomas M Suchyna
Department of Physiology and Biophysics, Center for Single Molecule Biophysics, State University New York SUNY at Buffalo, Buffalo, NY 14214, USA
J Physiol 581:369-87. 2007..Spontaneous Ca2+ transients in mdx mouse cells are sensitive to depolarization and are inhibited by the specific MSC inhibitor GsMTx4, in both the D and L forms... - Microfluidic actuation using electrochemically generated bubblesSusan Z Hua
Center for Bio MEMS, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14260, USA
Anal Chem 74:6392-6. 2002..Using such bubble valves, an eight-way multiplexer was fabricated and tested... - Neurite outgrowth from PC12 cells is enhanced by an inhibitor of mechanical channelsPhilip A Gottlieb
Physiology and Biophysics, 301 Cary Hall, SUNY at Buffalo, Buffalo, NY 14214, USA
Neurosci Lett 481:115-9. 2010..The inhibition of mechanosensitive channels by GsMTx4 may be a useful approach to accelerate regeneration of neurons in neurodegenerative diseases and spinal cord injury... - High-speed pressure clampStephen R Besch
Center for Single Molecule Biophysics, 320 Cary Hall, SUNY at Buffalo, Buffalo, NY 14214, USA
Pflugers Arch 445:161-6. 2002..5 ms for a 100-mmHg step, and the closed-loop settling time is 500-600 micros. Valve actuation latency is 120 micros. The system performance is illustrated for mechanically induced changes in patch capacitance... - Biophysics and structure of the patch and the gigasealThomas M Suchyna
State University of New York at Buffalo, Department of Physiology and Biophysics, Buffalo, New York 14214, USA
Biophys J 97:738-47. 2009..Voltage-dependent creep seems to arise from electroosmosis in the seal. Neutralization of negative charges on the seal membrane with low pH decreased the creep rate and reversed the direction of creep at positive pipette potentials... - A mechanosensitive ion channel regulating cell volumeSusan Z Hua
Department of Physiology and Biophysics, SUNY, Buffalo, NY 14214, USA
Am J Physiol Cell Physiol 298:C1424-30. 2010..The NRK cells form a stable platform from which to screen drugs that affect volume regulation via mechanosensory channels and as a sensitive system to clone the channel... - Dynamic effects of Hg2+-induced changes in cell volumeJinseok Heo
Department of Mechanical and Aerospace Engineering, SUNY Buffalo, 340 Jarvis Hall, Buffalo, NY 14260, USA
Cell Biochem Biophys 51:21-32. 2008.... - Mechanosensation and endothelin in astrocytes--hypothetical roles in CNS pathophysiologyLyle W Ostrow
Department of Physiology and Biophysics, S.U.N.Y. at Buffalo, School of Medicine and Biomedical Sciences, Buffalo, NY 14214, USA
Brain Res Brain Res Rev 48:488-508. 2005.... - Volume cytometry: microfluidic sensor for high-throughput screening in real timeDaniel A Ateya
Bio-MEMS and Bio-Materials Laboratory, Department of Mechanical and Aerospace Engineering, SUNY-Buffalo, Buffalo, New York 14260, USA
Anal Chem 77:1290-4. 2005..The prototype chip made in silicon is inexpensive, reusable, and runs on low-voltage electrical power. The technology can be readily transferred to large arrays in plastic... - Connexins are mechanosensitiveLi Bao
Department of Physiology and Biophysics, University of Miami School of Medicine, Miami, Florida 33136, USA
Am J Physiol Cell Physiol 287:C1389-95. 2004..Physiologically, Cx46 hemichannels may assist accommodation of the ocular lens by providing a transient path for volume flow as the lens changes shape... - Flexoelectricity and elasticity of asymmetric biomembranesAlexander G Petrov
Department of Physiology and Biophysics, SUNY Buffalo, 320 Cary Hall, Buffalo, New York 14214, USA
Phys Rev E Stat Nonlin Soft Matter Phys 65:021905. 2002..The results are discussed with respect to data from atomic force microscopy studies of electromotility in biomembranes... - Maximum likelihood estimation of ion channel kinetics from macroscopic currentsLorin S Milescu
Department of Physiology and Biophysics, State University of New York, Buffalo, New York, USA
Biophys J 88:2494-515. 2005..Given appropriate stimulation protocols, our method chose a reasonable model size and topology... 
Microfluidic chip to produce temperature jumps for electrophysiologyThomas Pennell
Department of Mechanical and Aerospace Engineering, State University of New York Buffalo, Buffalo, New York 14260, USA
Anal Chem 80:2447-51. 2008..The heat-stimulated currents were observed using whole-cell and cell-attached patch configurations. The results demonstrated that the chip can provide rapid temperature jumps at the resolution of single-ion channels...- Revisiting TRPC1 and TRPC6 mechanosensitivityPhilip Gottlieb
Single Molecule Biophysics, SUNY, 301 Cary Hall, Buffalo, NY 14214, USA
Pflugers Arch 455:1097-103. 2008..In conclusion, although several TRPC channel members, including TRPC1 and TRPC6, have been recently proposed to form MscCa in vertebrate cells, the functional expression of these TRPC subunits in heterologous systems remains problematic... - A direct optimization approach to hidden Markov modeling for single channel kineticsF Qin
Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
Biophys J 79:1915-27. 2000.... - Voltage-induced membrane movementP C Zhang
HHMI Center for Single Molecule Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA
Nature 413:428-32. 2001..As salicylate blocks electromotility in cochlear outer hair cells at the same concentration, the role of prestin as a motor protein may need to be reassessed... - A fluorescence energy transfer-based mechanical stress sensor for specific proteins in situFanjie Meng
Center for Single Molecule Biophysics, Department of Physiology and Biophysics, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY 14214, USA
FEBS J 275:3072-87. 2008..When collagen-19 was labeled near its middle with stFRET, it expressed well in Caenorhabditis elegans, distributing similarly to hosts labeled with a terminal green fluorescent protein, and the worms behaved normally... - Drift-free atomic force microscopy measurements of cell height and mechanical propertiesChiara Spagnoli
Department of Physiology and Biophysics, University at Buffalo, State University of New York, 3435 Main Street, 301 Cary Hall, Buffalo, NY 14214, USA
Rev Sci Instrum 78:036111. 2007..1 Hz. As a proof of concept, we applied the SaM to study the response of rat astrocytes to osmotic stress, observing dimensional and constitutive changes during volume regulation... - Desensitization of mechano-gated K2P channelsEric Honore
Single Molecule Biophysics, 301 Cary Hall, University at Buffalo, State University of New York, Buffalo, NY 14214, USA
Proc Natl Acad Sci U S A 103:6859-64. 2006..Desensitization and its regulation by chemical messengers is predicted to condition the physiological role of K2P channels... - Adapting the Quesant Nomad atomic force microscope for biology and patch-clamp atomic force microscopyS Besch
Huges Center for Single Molecule Biophysics, Physiology and Biophysical Sciences, SUNY at Buffalo, Buffalo, NY 14214, USA
Cell Biochem Biophys 39:195-210. 2003..The correlation of electrical and mechanical information allows signal averaging and measures sub-Angstrom, sub-millisecond electromotile responses from cells... - Extracting dwell time sequences from processive molecular motor dataLorin S Milescu
Department of Physiology and Biophysics, State University of New York, Buffalo, New York, USA
Biophys J 91:3135-50. 2006..The kinetic consequences of the extracted dwell sequence can be further analyzed in detail. We show results from analyzing simulated and experimental kinesin and myosin motor data. The algorithm is implemented in the free QuB software... - Maximum likelihood estimation of molecular motor kinetics from staircase dwell-time sequencesLorin S Milescu
Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York, USA
Biophys J 91:1156-68. 2006..The algorithm was tested with simulated data, and implemented in the free QuB software... - Dynamic properties of stretch-activated K+ channels in adult rat atrial myocytesWeizhen Niu
Department of Physiology, Capital University of Medical Sciences, 100054, Beijing, People's Republic of China
Prog Biophys Mol Biol 82:121-35. 2003..The time dependent behavior of these SACs is generally consistent with whole-cell currents observed in chick and rat ventricular cells, although the net current was outward rather than inward... - Effects of stretch-activated channel blockers on [Ca2+]i and muscle damage in the mdx mouseElla W Yeung
School of Medical Sciences and Institute for Biomedical Research, University of Sydney F13, NSW 2006, Australia
J Physiol 562:367-80. 2005..This result suggests that blockers of stretch-activated channels may protect against muscle damage in the intact mdx mouse... - Solution structure of peptide toxins that block mechanosensitive ion channelsRobert E Oswald
Department of Molecular Medicine, Cornell University, C3 167 Veterinary Medical Center, Ithaca, NY 14853, USA
J Biol Chem 277:34443-50. 2002..The spatial arrangement of charged residues that are unique to GsMTx-4 and GsMTx-2 may underlie the selectivity of these peptides... - Angiotensin II and myosin light-chain phosphorylation contribute to the stretch-induced slow force response in human atrial myocardiumJens Kockskamper
Division of Cardiology, Medical University of Graz, Auenbruggerplatz 15, A 8036 Graz, Austria
Cardiovasc Res 79:642-51. 2008..The functional effects of stretch on human atrium, however, are poorly understood. Thus, we characterized the stretch-induced force response in human atrium and evaluated the underlying cellular mechanisms... - Is lipid bilayer binding a common property of inhibitor cysteine knot ion-channel blockers?Yevgen O Posokhov
Biophys J 93:L20-2. 2007..The high variability of 5 kcal/mole in observed DeltaG shows that bilayer partitioning is not a universal property of the ICK peptides interacting with ion channels... - Ca2+ influx through mechanosensitive channels inhibits neurite outgrowth in opposition to other influx pathways and release from intracellular storesBridget T Jacques-Fricke
Department of Anatomy, University of Wisconsin, Madison, Wisconsin 53706, USA
J Neurosci 26:5656-64. 2006.... - Streptomycin reduces stretch-induced membrane permeability in muscles from mdx miceNicholas P Whitehead
School of Medical Sciences and Institute for Biomedical Research, University of Sydney F13, Sydney, NSW, Australia
Neuromuscul Disord 16:845-54. 2006..The results suggest that Ca(2+) entry through SACs activates Ca(2+) -dependent pathways, which are the main cause of the increased membrane permeability in mdx muscle... - Up- and down-regulation of the mechano-gated K(2P) channel TREK-1 by PIP (2) and other membrane phospholipidsJean Chemin
Institut de Genomique Fonctionnelle, UPR 2580 CNRS, 141 rue de la Cardonille, 34094, Montpellier Cedex 05, France
Pflugers Arch 455:97-103. 2007..This is, to our knowledge, the first report of an ion channel dually regulated by membrane phospholipids... - Retaining your identity under stressFrederick Sachs
U MD, College Park
Nat Struct Biol 9:636-7. 2002 - The slow force response to stretch in atrial and ventricular myocardium from human heart: functional relevance and subcellular mechanismsJens Kockskamper
Department of Cardiology, Medical University of Graz, Auenbruggerplatz 15, Graz, Austria
Prog Biophys Mol Biol 97:250-67. 2008..Increased myofilament Ca2+ sensitivity through MLCK-induced phosphorylation of MLC2 is a novel mechanism contributing to the SFR in both atrium and ventricle...
Research Grants
- AFM Of Voltage And Mechanically Gated ChannelsFrederick Sachs; Fiscal Year: 2004..The local electrical field around S4 will be measured by the electrically generated force as a function of the valence at known positions of S4. ..
- MECHANICAL TRANSDUCTION BY CARDIOCYTESFrederick Sachs; Fiscal Year: 2006..Aim 4. Measure the binding of peptides to lipid membranes using tryptophan fluorescence. Aim 5. Solve the 3D NMR structure of selected peptides. ..
- MECHANICAL TRANSDUCTION BY CARDIOCYTESFrederick Sachs; Fiscal Year: 2007..This knowledge will help pave the way for channel isolation by affinity chromatography and rational drug design. ..
- MECHANICAL TRANSDUCTION BY CARDIOCYTESFrederick Sachs; Fiscal Year: 2007..This knowledge will help pave the way for channel isolation by affinity chromatography and rational drug design. ..
- Cell mechanics and mechanical transduction by ion channelsFrederick Sachs; Fiscal Year: 2009..This proposal analyses how stresses are distributed in molecules, membranes and cells and how that leads to signal transduction by ion channels. ..
- MECHANICAL TRANSDUCTION BY CARDIOCYTESFrederick Sachs; Fiscal Year: 1999....
- MECHANICAL TRANSDUCTION BY CARDIOCYTESFrederick Sachs; Fiscal Year: 2003..Using the patch clamp and imaging of the patch we will measure the membrane mechanics of patches and the activation of endogenous and cloned MSCs as a function of the status of the cytoskeleton and extracellular matrix. ..
- Cell mechanics and mechanical transduction by ion channelsFrederick Sachs; Fiscal Year: 2010..This proposal analyses how stresses are distributed in molecules, membranes and cells and how that leads to signal transduction by ion channels. ..