BRUCE SACHAIS

Summary

Affiliation: University of Pennsylvania
Country: USA

Publications

  1. pmc Dynamic antibody-binding properties in the pathogenesis of HIT
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 120:1137-42. 2012
  2. pmc Rational design and characterization of platelet factor 4 antagonists for the study of heparin-induced thrombocytopenia
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Pearlman School of Medicine, University of Pennsylvania, 605A Stellar Chance Labs, 422 Curie Blvd, Philadelphia, PA 19104, USA
    Blood 119:5955-62. 2012
  3. ncbi request reprint Elimination of platelet factor 4 (PF4) from platelets reduces atherosclerosis in C57Bl/6 and apoE-/- mice
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Thromb Haemost 98:1108-13. 2007
  4. ncbi request reprint Platelet-endothelial interactions in atherosclerosis
    B S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3620 Hamilton Walk, 207A John Morgan Building, Philadelphia, PA 19104, USA
    Curr Atheroscler Rep 3:412-6. 2001
  5. ncbi request reprint Long-term effects of LDL apheresis in patients with severe hypercholesterolemia
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Clin Apher 20:252-5. 2005
  6. ncbi request reprint Platelet factor 4 binds to low-density lipoprotein receptors and disrupts the endocytic machinery, resulting in retention of low-density lipoprotein on the cell surface
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Blood 99:3613-22. 2002
  7. ncbi request reprint The costs of disease
    D S Young
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 4283, USA
    Clin Chem 46:955-66. 2000
  8. ncbi request reprint The alpha-defensins stimulate proteoglycan-dependent catabolism of low-density lipoprotein by vascular cells: a new class of inflammatory apolipoprotein and a possible contributor to atherogenesis
    A A Higazi
    Departments of Clinical Biochemistry, Surgery, and Medicine and the Center for Research, Prevention and Treatment of Atherosclerosis, Hebrew University Hadassah Medical Centers, Jerusalem, Israel
    Blood 96:1393-8. 2000
  9. ncbi request reprint Blood transfusion costs by diagnosis-related groups in 60 university hospitals in 1995
    L C Jefferies
    Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Transfusion 41:522-9. 2001
  10. doi request reprint Cryopreservation of canine platelets
    E H Appleman
    Schools of Veterinary Medicine and Medicine, University of Pennsylvania, Philadelphia, PA, USA
    J Vet Intern Med 23:138-45. 2009

Research Grants

  1. EFFECT OF PF4 ON LIPOPROTEIN METABOLISM/ATHEROSCLEROSIS
    BRUCE SACHAIS; Fiscal Year: 2004
  2. Proatherogenic properties of platelet fator 4
    BRUCE SACHAIS; Fiscal Year: 2009
  3. Drugs targeting intact lipoprotein receptors
    BRUCE SACHAIS; Fiscal Year: 2004
  4. Proatherogenic properties of platelet factor 4
    BRUCE SACHAIS; Fiscal Year: 2007
  5. Proatherogenic properties of platelet fator 4
    Bruce S Sachais; Fiscal Year: 2010

Collaborators

Detail Information

Publications25

  1. pmc Dynamic antibody-binding properties in the pathogenesis of HIT
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 120:1137-42. 2012
    ..These differences suggest the potential importance of epitope specificity in the pathogenesis of HIT...
  2. pmc Rational design and characterization of platelet factor 4 antagonists for the study of heparin-induced thrombocytopenia
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Pearlman School of Medicine, University of Pennsylvania, 605A Stellar Chance Labs, 422 Curie Blvd, Philadelphia, PA 19104, USA
    Blood 119:5955-62. 2012
    ..PF4As provide new tools to probe the pathophysiology of HIT. They also may provide insight into the development of novel, disease-specific therapeutics for the treatment of thromboembolic complications in HIT...
  3. ncbi request reprint Elimination of platelet factor 4 (PF4) from platelets reduces atherosclerosis in C57Bl/6 and apoE-/- mice
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    Thromb Haemost 98:1108-13. 2007
    ..These data demonstrate, for the first time, that the platelet specific chemokine PF4 promotes atherosclerotic lesion development in vivo...
  4. ncbi request reprint Platelet-endothelial interactions in atherosclerosis
    B S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3620 Hamilton Walk, 207A John Morgan Building, Philadelphia, PA 19104, USA
    Curr Atheroscler Rep 3:412-6. 2001
    ....
  5. ncbi request reprint Long-term effects of LDL apheresis in patients with severe hypercholesterolemia
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, Division of Transfusion Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA
    J Clin Apher 20:252-5. 2005
    ..These data support the use of LDL-apheresis in patients with FH, as well as medication-intolerant patients that have elevated LDL cholesterol despite maximal pharmacological treatment...
  6. ncbi request reprint Platelet factor 4 binds to low-density lipoprotein receptors and disrupts the endocytic machinery, resulting in retention of low-density lipoprotein on the cell surface
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA
    Blood 99:3613-22. 2002
    ..Retention of LDL on cell surfaces may facilitate proatherogenic modifications and support an expanded role for platelets in the pathogenesis of atherosclerosis...
  7. ncbi request reprint The costs of disease
    D S Young
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 4283, USA
    Clin Chem 46:955-66. 2000
    ..To date there have been no studies identifying and comparing the component costs to treat a large number of diseases for hospitalized inpatients...
  8. ncbi request reprint The alpha-defensins stimulate proteoglycan-dependent catabolism of low-density lipoprotein by vascular cells: a new class of inflammatory apolipoprotein and a possible contributor to atherogenesis
    A A Higazi
    Departments of Clinical Biochemistry, Surgery, and Medicine and the Center for Research, Prevention and Treatment of Atherosclerosis, Hebrew University Hadassah Medical Centers, Jerusalem, Israel
    Blood 96:1393-8. 2000
    ..Blood. 2000;96:1393-1398)..
  9. ncbi request reprint Blood transfusion costs by diagnosis-related groups in 60 university hospitals in 1995
    L C Jefferies
    Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, USA
    Transfusion 41:522-9. 2001
    ..Rank ordering of DRGs by transfusion costs and interinstitutional comparisons of these costs may lead to the selection of DRGs for further analysis of the process of blood transfusion...
  10. doi request reprint Cryopreservation of canine platelets
    E H Appleman
    Schools of Veterinary Medicine and Medicine, University of Pennsylvania, Philadelphia, PA, USA
    J Vet Intern Med 23:138-45. 2009
    ..Platelet cryopreservation allows long-term storage and immediate availability of transfusion products...
  11. ncbi request reprint Prothrombotic factors enhance heparin-induced thrombocytopenia and thrombosis in vivo in a mouse model
    M P Reilly
    Cardeza Foundation for Hematologic Research, Thomas Jefferson University, Philadelphia, PA 19107, USA
    J Thromb Haemost 4:2687-94. 2006
    ..However, anti-PF4/heparin antibodies are far more common than is HIT/T and there is less understanding of the factors that contribute to thrombosis in only a subset of patients...
  12. ncbi request reprint Heparin-induced thrombocytopenia/thrombosis in a transgenic mouse model requires human platelet factor 4 and platelet activation through FcgammaRIIA
    M P Reilly
    Cardeza Foundation for Hematologic Research, Department of Medicine, Jefferson Medical College, Philadelphia, PA 19107, USA
    Blood 98:2442-7. 2001
    ..This model should facilitate the identification of factors that modulate disease expression and the testing of novel therapeutic interventions...
  13. ncbi request reprint Comparative costs of treating adults and children within selected diagnosis-related groups
    Donald S Young
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 4283, USA
    Clin Chem 48:150-60. 2002
    ..Likewise, there have been no studies evaluating the costs of the various components of hospitalization (e.g., accommodation, laboratory, radiology, and drugs) among adult and pediatric populations...
  14. ncbi request reprint Heparin-induced thrombocytopenia: an autoimmune disorder regulated through dynamic autoantigen assembly/disassembly
    Douglas B Cines
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, and Division of Hematology, Children s Hospital of Philadelphia 19104, USA
    J Clin Apher 22:31-6. 2007
    ..They also demonstrate that this autoimmune disease can be modulated at the level of autoantigen formation and point to rational means to intervene proximal to thrombin generation...
  15. pmc PF4/heparin complexes are T cell-dependent antigens
    Shayela Suvarna
    Division of Hematology, DUMC Box 3486, Rm 301 Alex H Sands Bldg, Research Dr, Durham, NC 27710, USA
    Blood 106:929-31. 2005
    ..In contrast, athymic mice did not develop HIT-like antibodies. Taken together, these studies establish that PF4/heparin complexes are highly immunogenic and elicit self-reacting anti-PF4/heparin antibodies in a T cell-dependent manner...
  16. pmc Endothelial expression of E-selectin is induced by the platelet-specific chemokine platelet factor 4 through LRP in an NF-kappaB-dependent manner
    Guangyao Yu
    University of Pennsylvania, 207 John Morgan, Philadelphia, PA 19104, USA
    Blood 105:3545-51. 2005
    ..These results demonstrate that PF4 is able to increase expression of E-selectin by endothelial cells and represents another potential mechanism by which platelets may participate in atherosclerotic lesion progression...
  17. ncbi request reprint Ultralarge complexes of PF4 and heparin are central to the pathogenesis of heparin-induced thrombocytopenia
    Lubica Rauova
    Division of Hematology, Children s Hospital of Philadelphia, PA, USA
    Blood 105:131-8. 2005
    ....
  18. ncbi request reprint Effect of disease complications on hospital costs
    Donald S Young
    Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, 3400 Spruce St, Philadelphia, PA 19104 4283, USA
    Clin Chem 48:140-9. 2002
    ..To test the hypothesis that complications increase the use of resources in managing patients in hospitals, we examined the costs of managing patients with the same disease with and without complications...
  19. ncbi request reprint Platelet factor 4 enhances the binding of oxidized low-density lipoprotein to vascular wall cells
    Taher Nassar
    Department of Clinical Biochemistry, The Center for Research, Prevention, and Treatment of Atherosclerosis, Hadassah University Hospital and Hebrew University Hadassah Medical School, Jerusalem 91120, Israel
    J Biol Chem 278:6187-93. 2003
    ....
  20. ncbi request reprint Interactions of platelet factor 4 with the vessel wall
    Bruce S Sachais
    Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
    Semin Thromb Hemost 30:351-8. 2004
    ..Certainly, future studies using in vivo models to test biological relevance of each of these proposed mechanisms by which PF4 interacts with the vasculature are needed, as are studies to define the importance of PF4 binding to CXCR3B...
  21. ncbi request reprint The kringle stabilizes urokinase binding to the urokinase receptor
    Khalil Bdeir
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
    Blood 102:3600-8. 2003
    ..These data demonstrate an important role for the kringle in stabilizing the binding of scuPA to uPAR...
  22. ncbi request reprint Chemokines and thrombogenicity
    Michele P Lambert
    Division of Hematology, Children s Hospital of Philadelphia, 3615 Civic Center Blvd ARC Rm 316I, Philadelphia, PA 19104, USA
    Thromb Haemost 97:722-9. 2007
    ..This review focuses on the role of chemokines in platelet activation and thrombosis, particularly as it relates to atherosclerosis. Further studies to define this complex interaction are underway...
  23. ncbi request reprint Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment
    Helen V Yarovoi
    The Children s Hospital of Philadelphia, 1 Civic Center, ARC, Rm 317, Philadelphia, PA 19104, USA
    Blood 102:4006-13. 2003
    ..Our studies also suggest that platelet-released factor VIII is at least as potent as an equivalent plasma level and perhaps even more potent in an arterial thrombosis model...
  24. ncbi request reprint Antithrombotic thrombocytes: ectopic expression of urokinase-type plasminogen activator in platelets
    Dubravka Kufrin
    Children s Hospital of Philadelphia, 34th Street and Civic Center Boulevard, ARC Rm 317, Philadelphia, PA 19104, USA
    Blood 102:926-33. 2003
    ..These studies show the feasibility of delivering fibrinolytic agents to sites of incipient thrombus formation through selective storage in platelets and offer a new strategy to prevent thrombosis and hemorrhage...
  25. ncbi request reprint Platelet factor 4 localization in carotid atherosclerotic plaques: correlation with clinical parameters
    Stephanie Pitsilos
    Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, 19104, USA
    Thromb Haemost 90:1112-20. 2003
    ..These studies support the rationale for the chronic use of anti-platelet therapy in patients at risk for developing symptomatic atherosclerosis...

Research Grants8

  1. EFFECT OF PF4 ON LIPOPROTEIN METABOLISM/ATHEROSCLEROSIS
    BRUCE SACHAIS; Fiscal Year: 2004
    ..This research proposal is part of comprehensive training program designed to prepare the applicant for a career as an independent investigator in the field of vascular biology. ..
  2. Proatherogenic properties of platelet fator 4
    BRUCE SACHAIS; Fiscal Year: 2009
    ..Priority identification will be based on needs and resources assessment. The new curricula will be piloted through current educational programs. ..
  3. Drugs targeting intact lipoprotein receptors
    BRUCE SACHAIS; Fiscal Year: 2004
    ..Specific Aim 2: Create and validate biosensor-based analysis of LDL-R lipoparticles. In this aim, we will use LDL-R lipoparticles on the optical biosensor platform with the goal of measuring ligand-binding kinetics. ..
  4. Proatherogenic properties of platelet factor 4
    BRUCE SACHAIS; Fiscal Year: 2007
    ..into the role of PF4, the most abundant protein released by activated platelets, on the development of atherosclerosis, delineate the pathways by which this occurs in vivo, and suggest potential methods to intervene in atherogenesis ..
  5. Proatherogenic properties of platelet fator 4
    Bruce S Sachais; Fiscal Year: 2010
    ..into the role of PF4, the most abundant protein released by activated platelets, on the development of atherosclerosis, delineate the pathways by which this occurs in vivo, and suggest potential methods to intervene in atherogenesis ..