John B Sacci

Summary

Affiliation: University of Maryland
Country: USA

Publications

  1. ncbi Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates
    Keith Limbach
    US Military Malaria Vaccine Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, USA
    Malar J 10:65. 2011
  2. ncbi Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals
    Thanh V Nguyen
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Malar J 8:235. 2009
  3. ncbi Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expression
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    Mol Biochem Parasitol 142:177-83. 2005
  4. ncbi Gene expression analysis during liver stage development of Plasmodium
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Int J Parasitol 32:1551-7. 2002
  5. ncbi Plasmodium falciparum infection and exoerythrocytic development in mice with chimeric human livers
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    Int J Parasitol 36:353-60. 2006
  6. ncbi Laser capture microdissection and molecular analysis of Plasmodium yoelii liver-stage parasites
    John B Sacci
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Mol Biochem Parasitol 119:285-9. 2002
  7. ncbi Plasmodium yoelii: axenic development of the parasite mosquito stages
    Johanna M Porter-Kelley
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Exp Parasitol 112:99-108. 2006
  8. ncbi High-throughput generation of P. falciparum functional molecules by recombinational cloning
    João Carlos Aguiar
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Genome Res 14:2076-82. 2004
  9. ncbi CD8+ T lymphocytes protective against malaria liver stages are primed in skin-draining lymph nodes
    Sumana Chakravarty
    Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe Street, Baltimore, Maryland 21205, USA
    Nat Med 13:1035-41. 2007
  10. ncbi Functional immunoassays using an in-vitro malaria liver-stage infection model: where do we go from here?
    Brent L House
    US Military Malaria Vaccine Program, Naval Medical Research Center Walter Reed Army Institute of Research, Silver Spring, MD 21737, USA
    Trends Parasitol 25:525-33. 2009

Research Grants

  1. Development of a chimeric mouse model for the analysis of Plasmodium liver stages
    John Sacci; Fiscal Year: 2007

Collaborators

Detail Information

Publications19

  1. ncbi Identification of two new protective pre-erythrocytic malaria vaccine antigen candidates
    Keith Limbach
    US Military Malaria Vaccine Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, USA
    Malar J 10:65. 2011
    ..In this study, the efficacy of three pre-erythrocytic stage malaria antigens was evaluated in a Plasmodium yoelii/mouse protection model...
  2. ncbi Characterization of immunoglobulin G antibodies to Plasmodium falciparum sporozoite surface antigen MB2 in malaria exposed individuals
    Thanh V Nguyen
    Department of Molecular Biology and Biochemistry, University of California, Irvine, CA 92697, USA
    Malar J 8:235. 2009
    ..falciparum infected irradiated mosquitoes. The anti-MB2 antibody response may be part of a robust protective response against the sporozoite...
  3. ncbi Transcriptional analysis of in vivo Plasmodium yoelii liver stage gene expression
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    Mol Biochem Parasitol 142:177-83. 2005
    ..This is the first comprehensive analysis of in vivo gene expression undertaken for the liver stage of P. yoelii, and provides insights into the differential expression of P. yoelii genes during this critical stage of development...
  4. ncbi Gene expression analysis during liver stage development of Plasmodium
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Int J Parasitol 32:1551-7. 2002
    ..While significant progress has been made to identify genes that are expressed during liver stage parasite development, a great deal more work remains to be done...
  5. ncbi Plasmodium falciparum infection and exoerythrocytic development in mice with chimeric human livers
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, 655 W Baltimore Street, Baltimore, MD 21201, USA
    Int J Parasitol 36:353-60. 2006
    ..Our results establish a stable murine model for P. falciparum that will have a wide utility for assessing the biology of the parasite, potential anti-malarial chemotherapeutic agents and vaccine design...
  6. ncbi Laser capture microdissection and molecular analysis of Plasmodium yoelii liver-stage parasites
    John B Sacci
    Malaria Program, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA
    Mol Biochem Parasitol 119:285-9. 2002
  7. ncbi Plasmodium yoelii: axenic development of the parasite mosquito stages
    Johanna M Porter-Kelley
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, MD 21201, USA
    Exp Parasitol 112:99-108. 2006
    ..Our cell free system for development of the mosquito stages of P. yoelii provides a simplified approach to generate sporozoites that may be for biological assays and genetic manipulations...
  8. ncbi High-throughput generation of P. falciparum functional molecules by recombinational cloning
    João Carlos Aguiar
    Malaria Program, Naval Medical Research Center, Silver Spring, Maryland 20910, USA
    Genome Res 14:2076-82. 2004
    ..falciparum genome and will provide for a resource for the malaria resource community distributed through public repositories...
  9. ncbi CD8+ T lymphocytes protective against malaria liver stages are primed in skin-draining lymph nodes
    Sumana Chakravarty
    Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N Wolfe Street, Baltimore, Maryland 21205, USA
    Nat Med 13:1035-41. 2007
    ..Therefore, we report an unexpected dichotomy in the tissue restriction of host responses during the development and execution of protective immunity to Plasmodium...
  10. ncbi Functional immunoassays using an in-vitro malaria liver-stage infection model: where do we go from here?
    Brent L House
    US Military Malaria Vaccine Program, Naval Medical Research Center Walter Reed Army Institute of Research, Silver Spring, MD 21737, USA
    Trends Parasitol 25:525-33. 2009
    ....
  11. ncbi Monoclonal antibody MG96 completely blocks Plasmodium yoelii development in Anopheles stephensi
    Rhoel R Dinglasan
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA
    Infect Immun 71:6995-7001. 2003
    ..Future investigation of the carbohydrate epitope as well as gene identification should provide valuable insight into the possible mechanisms of ookinete attachment and invasion of mosquito midgut epithelial cells...
  12. ncbi Plasmodium falciparum liver stage antigen-1 is cross-linked by tissue transglutaminase
    William S Nicoll
    USMMVP, Malaria Department, NMRC, Silver Spring, MD 20910, USA
    Malar J 10:14. 2011
    ..The LSA-1 protein has been described as a flocculent mass, but its role in parasite development has not been determined...
  13. ncbi Subcellular localization of rickettsial invasion protein, InvA
    Jariyanart Gaywee
    Department of Microbiology and Immunology, School of Medicine, University of Maryland, Baltimore, Maryland 21201, USA
    Am J Trop Med Hyg 68:92-6. 2003
    ..Protecting bacteria from a hazardous environment could enhance their viability and allow them to remain metabolically active, which is a necessary step for the rickettsial obligate intracellular lifestyle...
  14. ncbi Inhibition of liver-stage development assay
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA
    Methods Mol Med 72:517-20. 2002
  15. ncbi Hepatocyte perfusion, isolation, and culture
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA
    Methods Mol Med 72:503-5. 2002
  16. ncbi Hepatic portal branch inoculation
    John B Sacci
    Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA
    Methods Mol Med 72:499-501. 2002
  17. ncbi Molecular mechanism of host specificity in Plasmodium falciparum infection: role of circumsporozoite protein
    Dharmendar Rathore
    Growth and Development Section, Laboratory of Malaria and Vector Research, NIAID, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 278:40905-10. 2003
    ..falciparum CSP (PfCSP). We suggest that a difference in the binding affinity for the liver cell receptor is a mechanism involved in maintaining the host specificity by the malaria parasite...
  18. ncbi A proteomic view of the Plasmodium falciparum life cycle
    Laurence Florens
    Department of Cell Biology, The Scripps Research Institute, SR-11, 10550 North Torrey Pines Road, La Jolla, California 92037, USA
    Nature 419:520-6. 2002
    ..The detection of chromosomal clusters encoding co-expressed proteins suggested a potential mechanism for controlling gene expression...
  19. ncbi Binding and invasion of liver cells by Plasmodium falciparum sporozoites. Essential involvement of the amino terminus of circumsporozoite protein
    Dharmendar Rathore
    Growth and Development Section, Laboratory of Malaria and Vector Research, NIAID/National Institutes of Health, 4 Center Drive MSC 0425, Bethesda, MD 20892-0425, USA
    J Biol Chem 277:7092-8. 2002
    ..This indicates that both assays are measuring interrelated phenomenon and points to the essential involvement for the amino-terminal portion of the CS protein in these processes...

Research Grants1

  1. Development of a chimeric mouse model for the analysis of Plasmodium liver stages
    John Sacci; Fiscal Year: 2007
    ..falciparum proteome and transcriptome of sporozoites, merozoites, trophozoites and gametocytes. This will then produce a truly comprehensive picture of gene expression during the lifecycle of human malaria parasites. ..