Tracey Ruhlman

Summary

Affiliation: University of Central Florida
Country: USA

Publications

  1. pmc Complete plastid genome sequence of the chickpea (Cicer arietinum) and the phylogenetic distribution of rps12 and clpP intron losses among legumes (Leguminosae)
    Robert K Jansen
    Section of Integrative Biology and Institute of Cellular and Molecular Biology, Biological Laboratories 404, University of Texas, Austin, TX 78712, USA
    Mol Phylogenet Evol 48:1204-17. 2008
  2. pmc Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids
    Robert K Jansen
    Section of Integrative Biology and Institute of Cellular and Molecular Biology, Patterson Laboratories 141, University of Texas, Austin, TX 78712, USA
    BMC Evol Biol 6:32. 2006
  3. ncbi request reprint Chloroplast genetic engineering via organogenesis or somatic embryogenesis
    Amit Dhingra
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, USA
    Methods Mol Biol 323:245-62. 2006
  4. ncbi request reprint Henry Daniell: chloroplast genetic engineering
    Henry Daniell
    Biotechnol J 1:31-3. 2006
  5. ncbi request reprint Production of biopharmaceuticals and vaccines in plants via the chloroplast genome
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Orlando, FL 32816 2364, USA
    Biotechnol J 1:1071-9. 2006
  6. pmc Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae
    Fu Hui Wu
    Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
    BMC Plant Biol 10:68. 2010
  7. pmc The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms
    Michael G Bausher
    USDA ARS, Horticultural Research Laboratory, Fort Pierce, FL 34945 3030, USA
    BMC Plant Biol 6:21. 2006
  8. pmc The complete chloroplast genome sequence of Gossypium hirsutum: organization and phylogenetic relationships to other angiosperms
    Seung Bum Lee
    Dept of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Building 20, Orlando, FL 32816 2364, USA
    BMC Genomics 7:61. 2006
  9. pmc Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques
    Jixiang Xia
    Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL 32827, USA
    BMC Biotechnol 11:62. 2011
  10. pmc Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms
    Tracey Ruhlman
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Building 20, Room 336, Orlando, FL 32816 2364, USA
    BMC Genomics 7:222. 2006

Collaborators

Detail Information

Publications58

  1. pmc Complete plastid genome sequence of the chickpea (Cicer arietinum) and the phylogenetic distribution of rps12 and clpP intron losses among legumes (Leguminosae)
    Robert K Jansen
    Section of Integrative Biology and Institute of Cellular and Molecular Biology, Biological Laboratories 404, University of Texas, Austin, TX 78712, USA
    Mol Phylogenet Evol 48:1204-17. 2008
    ..The availability of the complete chickpea plastid genome sequence also provides valuable information on intergenic spacer regions among legumes and endogenous regulatory sequences for plastid genetic engineering...
  2. pmc Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids
    Robert K Jansen
    Section of Integrative Biology and Institute of Cellular and Molecular Biology, Patterson Laboratories 141, University of Texas, Austin, TX 78712, USA
    BMC Evol Biol 6:32. 2006
    ..We sequenced the complete chloroplast genome of Vitis vinifera and used these data to assess relationships among 27 angiosperms, including nine taxa of rosids...
  3. ncbi request reprint Chloroplast genetic engineering via organogenesis or somatic embryogenesis
    Amit Dhingra
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, USA
    Methods Mol Biol 323:245-62. 2006
    ..Therefore, protocols of recent improvements in tissue culture, DNA delivery, and the novel vector designs are provided here in order to achieve highly efficient plastid transformation in Arabidopsis...
  4. ncbi request reprint Henry Daniell: chloroplast genetic engineering
    Henry Daniell
    Biotechnol J 1:31-3. 2006
  5. ncbi request reprint Production of biopharmaceuticals and vaccines in plants via the chloroplast genome
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Orlando, FL 32816 2364, USA
    Biotechnol J 1:1071-9. 2006
    ..Thus, transgenic chloroplasts are ideal bioreactors for production of functional human and animal therapeutic proteins in an environmentally friendly manner...
  6. pmc Complete chloroplast genome of Oncidium Gower Ramsey and evaluation of molecular markers for identification and breeding in Oncidiinae
    Fu Hui Wu
    Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
    BMC Plant Biol 10:68. 2010
    ..However, they are amenable to intergeneric and inter-specific crossing making phylogenetic identification very difficult. Molecular markers derived from the chloroplast genome can provide useful tools for phylogenetic resolution...
  7. pmc The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck var 'Ridge Pineapple': organization and phylogenetic relationships to other angiosperms
    Michael G Bausher
    USDA ARS, Horticultural Research Laboratory, Fort Pierce, FL 34945 3030, USA
    BMC Plant Biol 6:21. 2006
    ..We have sequenced the Citrus chloroplast genome to facilitate genetic improvement of this crop and to assess phylogenetic relationships among major lineages of angiosperms...
  8. pmc The complete chloroplast genome sequence of Gossypium hirsutum: organization and phylogenetic relationships to other angiosperms
    Seung Bum Lee
    Dept of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Building 20, Orlando, FL 32816 2364, USA
    BMC Genomics 7:61. 2006
    ..In addition, the sequence data were used to assess phylogenetic relationships among the major clades of rosids using cotton and 25 other completely sequenced angiosperm chloroplast genomes...
  9. pmc Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques
    Jixiang Xia
    Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, FL 32827, USA
    BMC Biotechnol 11:62. 2011
    ..In the present study, we evaluated the efficacy of biolistic gene transfer techniques in vivo using non-invasive bioluminescent imaging (BLI) methods...
  10. pmc Complete plastid genome sequence of Daucus carota: implications for biotechnology and phylogeny of angiosperms
    Tracey Ruhlman
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Building 20, Room 336, Orlando, FL 32816 2364, USA
    BMC Genomics 7:222. 2006
    ..Additionally, the sequence data add to the rapidly growing database of plastid genomes for assessing phylogenetic relationships among angiosperms...
  11. ncbi request reprint Chloroplast-derived vaccine antigens and other therapeutic proteins
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Bldg 20, Room 336, Orlando, FL 32816 2364, USA
    Vaccine 23:1779-83. 2005
    ..1% total leaf protein) are attractive features for therapeutic protein production. Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography...
  12. pmc Breakthrough in chloroplast genetic engineering of agronomically important crops
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Orlando, FL 32816 2364, USA
    Trends Biotechnol 23:238-45. 2005
    ..This review focuses on recent exciting developments in this field and offers directions for further research and development...
  13. ncbi request reprint Complete chloroplast genome sequences of Solanum bulbocastanum, Solanum lycopersicum and comparative analyses with other Solanaceae genomes
    Henry Daniell
    Department of Molecular Biology and Microbiology, Biomolecular Science, University of Central Florida, 4000 Central Florida Blvd, Bldg 20, Room 336, Orlando, FL 32816 2364, USA
    Theor Appl Genet 112:1503-18. 2006
    ..It is possible that there has been a loss of conserved editing sites in potato and tomato...
  14. pmc Molecular strategies for gene containment in transgenic crops
    Henry Daniell
    University of Central Florida, Department of Molecular Biology and Microbiology, 12722 Research Parkway, Orlando FL 32826 3227, USA
    Nat Biotechnol 20:581-6. 2002
    ..As yet, however, no strategy has proved broadly applicable to all crop species, and a combination of approaches may prove most effective for engineering the next generation of GM crops...
  15. pmc Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, Orlando, FL 32816 2364, USA
    BMC Biotechnol 9:33. 2009
    ..Therefore, in this study, human Insulin like Growth Factor-1 is expressed in transgenic chloroplasts for evaluation of structural identity and function...
  16. ncbi request reprint Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants
    H Daniell
    Dept Molecular Biology and Microbiology and Center for Discovery of Drugs and Diagnostics, University of Central Florida, 12 722 Research Parkway, Orlando, FL 32826, USA
    Trends Plant Sci 6:219-26. 2001
    ..Plant-derived biopharmaceuticals are cheap to produce and store, easy to scale up for mass production, and safer than those derived from animals. Here, we discuss recent developments in this field and possible environmental concerns...
  17. ncbi request reprint Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection
    H Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826 3227, USA
    Curr Genet 39:109-16. 2001
    ..The use of naturally occurring genes in spinach for selection, in addition to gene containment, should ease public concerns regarding GM crops...
  18. pmc Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts
    H Daniell
    Department of Molecular Biology and Microbiology and Center for Discovery of Drugs and Diagnostics, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826 3227, USA
    J Mol Biol 311:1001-9. 2001
    ....
  19. pmc Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology
    Henry Daniell
    Dept of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32826 3227, USA
    Trends Plant Sci 7:84-91. 2002
    ....
  20. pmc Multigene engineering: dawn of an exciting new era in biotechnology
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, 12722 Research Parkway, Orlando, Florida 32826 3227, USA
    Curr Opin Biotechnol 13:136-41. 2002
    ..These achievements illustrate the potential of multigene engineering to realize benefits of the post-genomic revolution...
  21. pmc The complete nucleotide sequence of the cassava (Manihot esculenta) chloroplast genome and the evolution of atpF in Malpighiales: RNA editing and multiple losses of a group II intron
    Henry Daniell
    Department Molecular Biology and Microbiology, College of Medicine, University of Central Florida, 4000 Central Florida Blvd, Biomolecular Science Bldg 20, Room 336, Orlando, FL 32816 2364, USA
    Theor Appl Genet 116:723-37. 2008
    ....
  22. pmc Plant-made vaccine antigens and biopharmaceuticals
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, College of Medicine, 336 Biomolecular Science Building, Orlando, FL 32816 2364, USA
    Trends Plant Sci 14:669-79. 2009
    ....
  23. pmc Transgene containment by maternal inheritance: effective or elusive?
    Henry Daniell
    Department of Molecular Biology and Microbiology, Biomolecular Science Building 20, Room 336, University of Central Florida, Orlando, FL 32816 2364, USA
    Proc Natl Acad Sci U S A 104:6879-80. 2007
  24. pmc The complete nucleotide sequence of the coffee (Coffea arabica L.) chloroplast genome: organization and implications for biotechnology and phylogenetic relationships amongst angiosperms
    Nalapalli Samson
    University of Central Florida, Department of Molecular Biology and Microbiology, Biomolecular Science, Building 20, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 5:339-53. 2007
    ..The availability of the complete chloroplast genome of coffee provides regulatory and intergenic spacer sequences for utilization in chloroplast genetic engineering to improve this important crop...
  25. pmc The role of heterologous chloroplast sequence elements in transgene integration and expression
    Tracey Ruhlman
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
    Plant Physiol 152:2088-104. 2010
    ....
  26. ncbi request reprint Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals
    B De Cosa
    Department of Molecular Biology and Microbiology, University of Central Florida, 12722 Research Parkway, Orlando, FL 32826-3227, USA
    Nat Biotechnol 19:71-4. 2001
    ..Demonstration of expression of an operon in transgenic plants paves the way to engineering new pathways in plants in a single transformation event...
  27. pmc Chloroplast vector systems for biotechnology applications
    Dheeraj Verma
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
    Plant Physiol 145:1129-43. 2007
  28. pmc Stable transformation of the cotton plastid genome and maternal inheritance of transgenes
    Shashi Kumar
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Bldg 20, Room 336, Orlando, FL 32816 2364, USA
    Plant Mol Biol 56:203-16. 2004
    ..Successful transformation of the chloroplast genome should address concerns about transgene escape, insects developing resistance, inadequate insect control and promote public acceptance of genetically modified cotton...
  29. pmc Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi
    G DeGray
    Department of Molecular Biology and Microbiology, 12722 Research Parkway, University of Central Florida, Orlando, FL 32826-3227, USA
    Plant Physiol 127:852-62. 2001
    ..Genetically engineering crop plants for disease resistance via the chloroplast genome instead of the nuclear genome is desirable to achieve high levels of expression and to prevent pollen-mediated escape of transgenes...
  30. pmc Plastid-expressed betaine aldehyde dehydrogenase gene in carrot cultured cells, roots, and leaves confers enhanced salt tolerance
    Shashi Kumar
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32816 2364, USA
    Plant Physiol 136:2843-54. 2004
    ..Demonstration of plastid transformation via somatic embryogenesis utilizing non-green tissues as recipients of foreign DNA for the first time overcomes two of the major obstacles in extending this technology to important crop plants...
  31. ncbi request reprint Chloroplast genetic engineering to improve agronomic traits
    Henry Daniell
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL, USA
    Methods Mol Biol 286:111-38. 2005
    ..This review provides protocols for engineering agronomic traits like insect, herbicide and disease resistance; salt and drought tolerance; and phyto-remediation via chloroplast genome...
  32. pmc Expression of cholera toxin B-proinsulin fusion protein in lettuce and tobacco chloroplasts--oral administration protects against development of insulitis in non-obese diabetic mice
    Tracey Ruhlman
    University of Central Florida, Department of Molecular Biology and Microbiology, Biomolecular Science, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 5:495-510. 2007
    ..This opens up the possibility for the low-cost production and delivery of human therapeutic proteins, and a strategy for the treatment of various other autoimmune diseases...
  33. pmc Chloroplast-derived vaccine antigens and biopharmaceuticals: expression, folding, assembly and functionality
    S Chebolu
    Department of Molecular Biology and Microbiology, University of Central Florida, 336 Biomolecular Science Bldg 20, Orlando, FL 32816 2360, USA
    Curr Top Microbiol Immunol 332:33-54. 2009
    ..Thus, transgenic chloroplasts are ideal bio-reactors for production of functional human and animal therapeutic proteins in an environmentally friendly manner...
  34. doi request reprint A protocol for expression of foreign genes in chloroplasts
    Dheeraj Verma
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Biomolecular Science, Building 20, Room 336, Orlando, Florida 32816 2364, USA
    Nat Protoc 3:739-58. 2008
    ..CTT is eco-friendly because transgenes are maternally inherited in most crop plants...
  35. pmc Chloroplast-derived enzyme cocktails hydrolyse lignocellulosic biomass and release fermentable sugars
    Dheeraj Verma
    Department of Molecular Biology and Microbiology, Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 8:332-50. 2010
    ..Limitations of higher cost and lower production capacity of fermentation systems are addressed by chloroplast-derived enzyme cocktails...
  36. pmc Phytoremediation of organomercurial compounds via chloroplast genetic engineering
    Oscar N Ruiz
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida 32816 2360, USA
    Plant Physiol 132:1344-52. 2003
    ..Chloroplast transformation may also have application to other metals that affect chloroplast function...
  37. ncbi request reprint Engineering the chloroplast genome for hyperexpression of human therapeutic proteins and vaccine antigens
    Shashi Kumar
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, USA
    Methods Mol Biol 267:365-83. 2004
    ..Suitable examples for application of chloroplast genetic engineering in human medicine are provided...
  38. doi request reprint Chloroplast-derived vaccine antigens and biopharmaceuticals: protocols for expression, purification, or oral delivery and functional evaluation
    N Dolendro Singh
    N Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Orlando, FL, USA
    Methods Mol Biol 483:163-92. 2009
    ....
  39. pmc Stable expression of Gal/GalNAc lectin of Entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis
    Seethamahalakshmi Chebolu
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Bldg 20, Room 336, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 5:230-9. 2007
    ..Such high levels of expression and immunogenicity should facilitate the development of a less expensive amoebiasis vaccine...
  40. pmc Engineering cytoplasmic male sterility via the chloroplast genome by expression of {beta}-ketothiolase
    Oscar N Ruiz
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida 32816 2364 USA
    Plant Physiol 138:1232-46. 2005
    ....
  41. pmc Characterization of heterologous multigene operons in transgenic chloroplasts: transcription, processing, and translation
    Tania Quesada-Vargas
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, Florida 32816 2364, USA
    Plant Physiol 138:1746-62. 2005
    ..Knowledge of such factors would enable engineering of foreign pathways independent of the chloroplast complex posttranscriptional regulatory machinery...
  42. pmc Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery
    Abdoreza Davoodi-Semiromi
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 8:223-42. 2010
    ....
  43. pmc Receptor-mediated oral delivery of a bioencapsulated green fluorescent protein expressed in transgenic chloroplasts into the mouse circulatory system
    Arati Limaye
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, 4000 Central Florida Blvd, Bldg 20, Rm 336, Orlando, Florida 32816 2364, USA
    FASEB J 20:959-61. 2006
    ..This report of receptor-mediated oral delivery of a foreign protein into the circulatory system opens the door for low-cost production and delivery of human therapeutic proteins...
  44. pmc Arabidopsis Tic40 expression in tobacco chloroplasts results in massive proliferation of the inner envelope membrane and upregulation of associated proteins
    Nameirakpam Dolendro Singh
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, Florida 32816 2364, USA
    Plant Cell 20:3405-17. 2008
    ....
  45. pmc Plant-based vaccine: mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge
    Vijay Koya
    Dept Molecular Biology and Microbiology, Biomolecular Science Bldg 20, Room 336, University of Central Florida, Central Florida Blvd, Orlando, FL 32816 2364, USA
    Infect Immun 73:8266-74. 2005
    ..These results demonstrate the immunogenic and immunoprotective properties of plant-derived anthrax vaccine antigen...
  46. pmc Effective plague vaccination via oral delivery of plant cells expressing F1-V antigens in chloroplasts
    Philip A Arlen
    Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32816 2364, USA
    Infect Immun 76:3640-50. 2008
    ..In addition, this is the first report of a plant-derived oral vaccine that protected animals from live Y. pestis challenge, bringing the likelihood of lower-cost vaccines closer to reality...
  47. pmc Field production and functional evaluation of chloroplast-derived interferon-alpha2b
    Philip A Arlen
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science, Orlando, FL 32816 2364, USA
    Plant Biotechnol J 5:511-25. 2007
    ....
  48. pmc Enhanced translation of a chloroplast-expressed RbcS gene restores small subunit levels and photosynthesis in nuclear RbcS antisense plants
    Amit Dhingra
    Department of Molecular Biology and Microbiology, University of Central Florida, 4000 Central Florida Boulevard, Biomolecular Science, Building 20, Room 336, Orlando, FL 32816 2364, USA
    Proc Natl Acad Sci U S A 101:6315-20. 2004
    ..These results have opened an avenue for using chloroplast engineering for the evaluation of foreign Rubisco genes in planta that eventually can result in achieving efficient photosynthesis and increased crop productivity...
  49. pmc Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop
    Jennifer Watson
    Department of Molecular Biology and Microbiology, University of Central Florida, Biomolecular Science Building 20, Room 336, Orlando, FL 32816 2364, USA
    Vaccine 22:4374-84. 2004
    ....
  50. pmc Oral delivery of bioencapsulated coagulation factor IX prevents inhibitor formation and fatal anaphylaxis in hemophilia B mice
    Dheeraj Verma
    Department of Molecular Biology and Microbiology, College of Medicine, University of Central Florida, Orlando, FL 32816 2364, USA
    Proc Natl Acad Sci U S A 107:7101-6. 2010
    ..This cost-effective and efficient approach of antigen delivery to the gut should be applicable to several genetic diseases that are prone to pathogenic antibody responses during treatment...
  51. ncbi request reprint Chloroplast-derived anthrax and other vaccine antigens: their immunogenic and immunoprotective properties
    Sushama Kamarajugadda
    Department of Molecular Biology and Microbiology, University of Central Florida, Bimolecular science Building 20, Room 336, Orlando, FL 32816 2364, USA
    Expert Rev Vaccines 5:839-49. 2006
    ..Chloroplast-derived vaccine antigens are efficacious against bacterial, fungal, viral and protozoan pathogens...
  52. ncbi request reprint Jumping genes and containment
    Henry Daniell
    Nat Biotechnol 21:374-5. 2003
  53. pmc Analysis of 81 genes from 64 plastid genomes resolves relationships in angiosperms and identifies genome-scale evolutionary patterns
    Robert K Jansen
    Section of Integrative Biology and Institute of Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA
    Proc Natl Acad Sci U S A 104:19369-74. 2007
    ..Moreover, a lineage-specific correlation was detected between rates of nucleotide substitutions, indels, and genomic rearrangements...
  54. pmc Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera, and comparative analyses with other grass genomes
    Christopher Saski
    Clemson University Genomics Institute, Clemson University, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, USA
    Theor Appl Genet 115:571-90. 2007
    ..Phylogenetic trees based on DNA sequences of 61 protein-coding genes of 38 taxa using both maximum parsimony and likelihood methods provide moderate support for a sister relationship between the subfamilies Erhartoideae and Pooideae...
  55. pmc Metabolic engineering of the chloroplast genome using the Echerichia coli ubiC gene reveals that chorismate is a readily abundant plant precursor for p-hydroxybenzoic acid biosynthesis
    Paul V Viitanen
    DuPont Experimental Station, Wilmington, Delaware 19880 0402, USA
    Plant Physiol 136:4048-60. 2004
    ....
  56. pmc Plastid transformation in the monocotyledonous cereal crop, rice (Oryza sativa) and transmission of transgenes to their progeny
    Sa Mi Lee
    School of Agricultural Biotechnology, Seoul National University, Seoul 151 921, Korea
    Mol Cells 21:401-10. 2006
    ....
  57. ncbi request reprint Complete chloroplast genome sequence of Gycine max and comparative analyses with other legume genomes
    Christopher Saski
    Clemson University Genomics Institute, Clemson University, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, USA
    Plant Mol Biol 59:309-22. 2005
    ..The presence of small repeats of psbA and rbcL in legumes that have lost one copy of the IR indicate that this loss has only occurred once during the evolutionary history of legumes...
  58. pmc Phytoremediation of mercury and organomercurials in chloroplast transgenic plants: enhanced root uptake, translocation to shoots, and volatilization
    Hussein S Hussein
    Department of Plant and Molecular Biology, University of California at Berkeley, 111 Koshland Hall, Berkeley, California 94720 3102, USA
    Environ Sci Technol 41:8439-46. 2007
    ..These investigations provide novel insights for improvement of plant tolerance and detoxification of mercury...

Research Grants8

  1. Expression of Human Therapeutic Proteins in Chloroplasts
    Henry Daniell; Fiscal Year: 2005
    ..Large scale and low cost production via transgenic chloroplasts should provide treatment to patients at an affordable cost and tobacco farmers alternate uses for this hazardous crop. ..
  2. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2006
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  3. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2009
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..
  4. Expression of Human Therapeutic Proteins in Transgenic Tobacco Chloroplasts
    Henry Daniell; Fiscal Year: 2010
    ..Successful completion of these studies would make transgenic chloroplasts ideal bioreactors for the production of safe and less expensive therapeutic proteins and open the door for in depth studies of membrane proteins. ..