Research Topics
| Edward RubySummaryAffiliation: University of Wisconsin Country: USA Publications
Research Grants
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Detail Information
Publications
Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual expansionsMark J Mandel
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison WI 53706 1521, USA
BMC Genomics 9:138. 2008..Building on the genome project of Vibrio fischeri ES114, we used a comparative approach to identify and investigate genes that had a high likelihood of sequence error...
Symbiotic conversations are revealed under genetic interrogationEdward G Ruby
Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Room 5203 Microbial Sciences Building, 1550 Linden Drive, Madison, Wisconsin 53706 1521, USA
Nat Rev Microbiol 6:752-62. 2008..In this Review, I discuss many of these developments, focusing on what has been discovered in five well-understood model systems...- Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congenersE G Ruby
Pacific Biomedical Research Center, Kewalo Marine Laboratory, and W M Keck Microbial Communities and Cell Signaling Program, University of Hawaii, Honolulu, HI 96813, USA
Proc Natl Acad Sci U S A 102:3004-9. 2005..fischeri ES114, which enters into a mutualistic symbiosis in the light organ of the bobtail squid, Euprymna scolopes. Analysis of this sequence has revealed surprising parallels with V. cholerae and other pathogens... - A single regulatory gene is sufficient to alter bacterial host rangeMark J Mandel
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, Wisconsin 53706, USA
Nature 458:215-8. 2009.... - A single qrr gene is necessary and sufficient for LuxO-mediated regulation in Vibrio fischeriTim Miyashiro
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, WI 53706 1521, USA
Mol Microbiol 77:1556-67. 2010..Our studies contribute to a better understanding of the ancestral state of these pathways in vibrios, as well as to the evolution and divergence of other sRNAs within different bacterial lineages... 
LitR, a new transcriptional activator in Vibrio fischeri, regulates luminescence and symbiotic light organ colonizationPat M Fidopiastis
Pacific Biomedical Research Center, University of Hawai i, Honolulu, 96813, USA
Mol Microbiol 45:131-43. 2002..Thus, the litR mutation appears to represent a novel class of mutations in which the loss of a regulatory gene function enhances the bacterium's competence in initiating a benign infection...- The Vibrio fischeri quorum-sensing systems ain and lux sequentially induce luminescence gene expression and are important for persistence in the squid hostClaudia Lupp
Pacific Biomedical Research Center, University of Hawai i, Manoa, 41 Ahui Street, Honolulu, HI 96813, USA
Mol Microbiol 50:319-31. 2003..Taken together, our results suggest that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expression of luminescence genes and possibly other colonization factors... - AinS quorum sensing regulates the Vibrio fischeri acetate switchSarah V Studer
Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, 5203 Microbial Sciences Building, Madison, WI 53706, USA
J Bacteriol 190:5915-23. 2008..This is the first report of quorum-sensing control of the acetate switch, and it indicates a metabolic connection between acetate utilization and cell density... - Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio associationCarlene K Chun
Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 105:11323-8. 2008.... - Contribution of pilA to competitive colonization of the squid Euprymna scolopes by Vibrio fischeriEric V Stabb
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
Appl Environ Microbiol 69:820-6. 2003..The competitive defect of pilA mutants suggests that cell surface molecules may play important roles in the initiation of beneficial symbioses in which animals must acquire symbionts from a mixed community of environmental bacteria... - Vibrio fischeri LuxS and AinS: comparative study of two signal synthasesClaudia Lupp
Pacific Biomedical Research Center, University of Hawaii at Manoa, 41 Ahui St, Honolulu, HI 96813, USA
J Bacteriol 186:3873-81. 2004..Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation and colonization competence; however, its quantitative contribution is small when compared to that of the AinS signal... - Vibrio fischeri uses two quorum-sensing systems for the regulation of early and late colonization factorsClaudia Lupp
Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, USA
J Bacteriol 187:3620-9. 2005..g., motility) by ain quorum sensing, whereas late colonization factors (e.g., luminescence) are preferentially regulated by lux quorum sensing... - Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosisAndrew M Wier
Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 107:2259-64. 2010..Thus, the persistent maintenance of the squid-vibrio symbiosis is tied to a dynamic diel rhythm that involves both partners... - Confocal microscopy of the light organ crypts in juvenile Euprymna scolopes reveals their morphological complexity and dynamic function in symbiosisLaura K Sycuro
Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin 53706, USA
J Morphol 267:555-68. 2006.... - H-NOX-mediated nitric oxide sensing modulates symbiotic colonization by Vibrio fischeriYanling Wang
Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
Proc Natl Acad Sci U S A 107:8375-80. 2010..Our data suggest that V. fischeri normally senses a host-generated NO signal through H-NOX(Vf) and modulates the expression of its iron uptake capacity during the early stages of the light-organ symbiosis... - Recognition between symbiotic Vibrio fischeri and the haemocytes of Euprymna scolopesSpencer V Nyholm
Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA
Environ Microbiol 11:483-93. 2009..These data suggest that regulation of host haemocyte binding by the symbiont may be one of many factors that contribute to specificity in this association... - GacA regulates symbiotic colonization traits of Vibrio fischeri and facilitates a beneficial association with an animal hostCheryl A Whistler
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
J Bacteriol 185:7202-12. 2003..This study establishes the role of GacA as a regulator of a beneficial animal-microbe association and indicates that GacA regulates utilization of growth substrates as well as other colonization traits... - Population dynamics of Vibrio fischeri during infection of Euprymna scolopesJessica McCann
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
Appl Environ Microbiol 69:5928-34. 2003..Further, these results indicated that, when exposed to low numbers of V. fischeri, the host may become colonized by only one or a few bacterial cells, suggesting that symbiotic infection is highly efficient... - FlrA, a sigma54-dependent transcriptional activator in Vibrio fischeri, is required for motility and symbiotic light-organ colonizationDeborah S Millikan
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
J Bacteriol 185:3547-57. 2003..Thus, the transcriptional activator FlrA plays an essential role in regulating motility, and apparently in modulating other symbiotic functions, in V. fischeri... - Alterations in Vibrio fischeri motility correlate with a delay in symbiosis initiation and are associated with additional symbiotic colonization defectsDeborah S Millikan
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
Appl Environ Microbiol 68:2519-28. 2002..Furthermore, the pleiotropic nature of class III HS strains provides evidence that motility is coregulated with other symbiotic determinants in V. fischeri... - The Vibrio fischeri sapABCDF locus is required for normal growth, both in culture and in symbiosisClaudia Lupp
University of Hawaii, Kewalo Marine Laboratory, 41 Ahui Street, Honolulu, HI 96813, USA
Arch Microbiol 179:57-65. 2002..Thus, while the sap locus is important to the normal growth of V. fischeri, it plays different physiological roles in growth and tissue colonization than it does in enteric pathogens... - A novel lux operon in the cryptically bioluminescent fish pathogen Vibrio salmonicida is associated with virulenceEric J Nelson
Department of Medical Microbiology and Immunology, 1300 University Ave, University of Wisconsin, Madison, WI 53706, USA
Appl Environ Microbiol 73:1825-33. 2007..Nevertheless, these findings document for the first time an association between a mutation in a structural lux gene and virulence, as well as provide a new molecular system to study Vibrio pathogenesis in a natural host... - Vibrio fischeri flagellin A is essential for normal motility and for symbiotic competence during initial squid light organ colonizationDeborah S Millikan
Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
J Bacteriol 186:4315-25. 2004..fischeri is a complex structure consisting of multiple flagellin subunits, including FlaA, which is essential both for normal flagellation and for motility, as well as for effective symbiotic colonization... - RP4-based plasmids for conjugation between Escherichia coli and members of the VibrionaceaeEric V Stabb
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
Methods Enzymol 358:413-26. 2002 - Characterization of htrB and msbB mutants of the light organ symbiont Vibrio fischeriDawn M Adin
University of Georgia, Department of Microbiology, 1000 Cedar Street, Athens, GA 30602, USA
Appl Environ Microbiol 74:633-44. 2008..7-fold during colonization when mixed with the parent. These data suggest that htrB2 and msbB play conserved general roles in vibrio biology, whereas htrB1 plays a more symbiosis-specific role in V. fischeri... - The GacA global regulator of Vibrio fischeri is required for normal host tissue responses that limit subsequent bacterial colonizationCheryl A Whistler
Department of Microbiology, University of New Hampshire, Durham, NH 03824 3587, USA
Cell Microbiol 9:766-78. 2007.... - Two-component response regulators of Vibrio fischeri: identification, mutagenesis, and characterizationElizabeth A Hussa
Department of Microbiology and Immunology, Loyola University Chicago, 2160 S First Avenue, Maywood, IL 60153, USA
J Bacteriol 189:5825-38. 2007..This work represents the first large-scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the importance of two-component signal transduction in bacterium-host interactions... - Transcriptome analysis of the Vibrio fischeri LuxR-LuxI regulonLuis Caetano M Antunes
Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
J Bacteriol 189:8387-91. 2007..This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production... - Vibrio fischeri and its host: it takes two to tangoKaren L Visick
Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA
Curr Opin Microbiol 9:632-8. 2006..fischeri, an expressed sequence tagged library for E. scolopes and the availability of dual-fluorescence markers and confocal microscopy to probe symbiotic structures and the dynamics of colonization...
Research Grants
- VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATIONEdward Ruby; Fiscal Year: 2007..The work proposed here will also provide new informationabout how specific, stable associationswith aquatic invertebrates may serve as environmentalreservoirs for human pathogenic Vibrio species. ..
- VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATIONEdward Ruby; Fiscal Year: 2006..The work proposed here will also provide new information about how specific, stable associations with aquatic invertebrates may serve as environmental reservoirs for human pathogenic Vibrio species. ..
- VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATIONEdward Ruby; Fiscal Year: 2004..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..
- COLONIZATION OF EPITHELIUM BY SYMBIOTIC VIBRIO FISCHERIEdward Ruby; Fiscal Year: 1999....
- VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATIONEdward G Ruby; Fiscal Year: 2010..We will investigate a natural bacterial association to define the signaling events that underlie a symbiont-induced daily remodeling of the host's tissues that leads to a healthy persistent interaction. ..