Edward Ruby

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Genetic determinants of swimming motility in the squid light-organ symbiont Vibrio fischeri
    Caitlin A Brennan
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin
    Microbiologyopen 2:576-94. 2013
  2. pmc Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual expansions
    Mark J Mandel
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison WI 53706 1521, USA
    BMC Genomics 9:138. 2008
  3. pmc Symbiotic conversations are revealed under genetic interrogation
    Edward G Ruby
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Room 5203 Microbial Sciences Building, 1550 Linden Drive, Madison, Wisconsin 53706 1521, USA
    Nat Rev Microbiol 6:752-62. 2008
  4. pmc Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners
    E G Ruby
    Pacific Biomedical Research Center, Kewalo Marine Laboratory, and W M Keck Microbial Communities and Cell Signaling Program, University of Hawaii, Honolulu, HI 96813, USA
    Proc Natl Acad Sci U S A 102:3004-9. 2005
  5. ncbi request reprint LitR, a new transcriptional activator in Vibrio fischeri, regulates luminescence and symbiotic light organ colonization
    Pat M Fidopiastis
    Pacific Biomedical Research Center, University of Hawai i, Honolulu, 96813, USA
    Mol Microbiol 45:131-43. 2002
  6. pmc A single qrr gene is necessary and sufficient for LuxO-mediated regulation in Vibrio fischeri
    Tim Miyashiro
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, WI 53706 1521, USA
    Mol Microbiol 77:1556-67. 2010
  7. pmc A single regulatory gene is sufficient to alter bacterial host range
    Mark J Mandel
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, Wisconsin 53706, USA
    Nature 458:215-8. 2009
  8. ncbi request reprint The Vibrio fischeri quorum-sensing systems ain and lux sequentially induce luminescence gene expression and are important for persistence in the squid host
    Claudia Lupp
    Pacific Biomedical Research Center, University of Hawai i, Manoa, 41 Ahui Street, Honolulu, HI 96813, USA
    Mol Microbiol 50:319-31. 2003
  9. pmc Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association
    Carlene K Chun
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:11323-8. 2008
  10. pmc Contribution of pilA to competitive colonization of the squid Euprymna scolopes by Vibrio fischeri
    Eric V Stabb
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    Appl Environ Microbiol 69:820-6. 2003

Collaborators

  • Eric V Stabb
  • Margaret J McFall-Ngai
  • Todd E Scheetz
  • Mark J Mandel
  • Tim Miyashiro
  • Henning Sørum
  • Timothy J Donohue
  • Cheryl A Whistler
  • Carol M Miyamoto
  • Carlene K Chun
  • KAREN VISICK
  • John Cronan
  • Claudia Lupp
  • Deborah S Millikan
  • Spencer V Nyholm
  • Caitlin A Brennan
  • Pat M Fidopiastis
  • Andrew M Wier
  • Yanling Wang
  • Amy L Schaefer
  • Dawn M Adin
  • Sarah V Studer
  • Eric J Nelson
  • Elizabeth A Hussa
  • Luis Caetano M Antunes
  • Laura K Sycuro
  • E Peter Greenberg
  • Jessica McCann
  • Krista A Thomasgard
  • Mattias C Gyllborg
  • Hans K Carlson
  • M Bento Soares
  • Sandra Splinter-Bondurant
  • Maria de Fatima Bonaldo
  • Irina Koroleva
  • Liliana Manzella
  • Jennifer L Reed
  • R Prisca Massengo-Tiassé
  • Hakeem Almabrazi
  • Einat Snir
  • Thomas L Casavant
  • Michael A Marletta
  • Bartley Brown
  • Yann S Dufour
  • Jennifer J Stewart
  • Bradford W Gibson
  • Daniel B Hall
  • Nancy J Phillips
  • Michael A Apicella
  • Ann M Stevens
  • Nan Qin
  • Cynthia L Darnell
  • Hege S Tunsjø
  • Rosana B R Ferreira
  • Mark Urbanowski
  • Edward A Meighen
  • Michael G Jobling
  • Robert E W Hancock

Detail Information

Publications32

  1. pmc Genetic determinants of swimming motility in the squid light-organ symbiont Vibrio fischeri
    Caitlin A Brennan
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin
    Microbiologyopen 2:576-94. 2013
    ..fischeri and furthered our understanding of the roles of flagellar motility and chemotaxis in colonization of the juvenile squid, including identifying 11 novel mutants unable to enter into a productive light-organ symbiosis. ..
  2. pmc Comparative genomics-based investigation of resequencing targets in Vibrio fischeri: focus on point miscalls and artefactual expansions
    Mark J Mandel
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison WI 53706 1521, USA
    BMC Genomics 9:138. 2008
    ..Building on the genome project of Vibrio fischeri ES114, we used a comparative approach to identify and investigate genes that had a high likelihood of sequence error...
  3. pmc Symbiotic conversations are revealed under genetic interrogation
    Edward G Ruby
    Department of Medical Microbiology and Immunology, University of Wisconsin Madison, Room 5203 Microbial Sciences Building, 1550 Linden Drive, Madison, Wisconsin 53706 1521, USA
    Nat Rev Microbiol 6:752-62. 2008
    ..In this Review, I discuss many of these developments, focusing on what has been discovered in five well-understood model systems...
  4. pmc Complete genome sequence of Vibrio fischeri: a symbiotic bacterium with pathogenic congeners
    E G Ruby
    Pacific Biomedical Research Center, Kewalo Marine Laboratory, and W M Keck Microbial Communities and Cell Signaling Program, University of Hawaii, Honolulu, HI 96813, USA
    Proc Natl Acad Sci U S A 102:3004-9. 2005
    ..fischeri ES114, which enters into a mutualistic symbiosis in the light organ of the bobtail squid, Euprymna scolopes. Analysis of this sequence has revealed surprising parallels with V. cholerae and other pathogens...
  5. ncbi request reprint LitR, a new transcriptional activator in Vibrio fischeri, regulates luminescence and symbiotic light organ colonization
    Pat M Fidopiastis
    Pacific Biomedical Research Center, University of Hawai i, Honolulu, 96813, USA
    Mol Microbiol 45:131-43. 2002
    ..Thus, the litR mutation appears to represent a novel class of mutations in which the loss of a regulatory gene function enhances the bacterium's competence in initiating a benign infection...
  6. pmc A single qrr gene is necessary and sufficient for LuxO-mediated regulation in Vibrio fischeri
    Tim Miyashiro
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, WI 53706 1521, USA
    Mol Microbiol 77:1556-67. 2010
    ..Our studies contribute to a better understanding of the ancestral state of these pathways in vibrios, as well as to the evolution and divergence of other sRNAs within different bacterial lineages...
  7. pmc A single regulatory gene is sufficient to alter bacterial host range
    Mark J Mandel
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, Madison, Wisconsin 53706, USA
    Nature 458:215-8. 2009
    ....
  8. ncbi request reprint The Vibrio fischeri quorum-sensing systems ain and lux sequentially induce luminescence gene expression and are important for persistence in the squid host
    Claudia Lupp
    Pacific Biomedical Research Center, University of Hawai i, Manoa, 41 Ahui Street, Honolulu, HI 96813, USA
    Mol Microbiol 50:319-31. 2003
    ..Taken together, our results suggest that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expression of luminescence genes and possibly other colonization factors...
  9. pmc Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association
    Carlene K Chun
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:11323-8. 2008
    ....
  10. pmc Contribution of pilA to competitive colonization of the squid Euprymna scolopes by Vibrio fischeri
    Eric V Stabb
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    Appl Environ Microbiol 69:820-6. 2003
    ..The competitive defect of pilA mutants suggests that cell surface molecules may play important roles in the initiation of beneficial symbioses in which animals must acquire symbionts from a mixed community of environmental bacteria...
  11. pmc AinS quorum sensing regulates the Vibrio fischeri acetate switch
    Sarah V Studer
    Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, 1550 Linden Drive, 5203 Microbial Sciences Building, Madison, WI 53706, USA
    J Bacteriol 190:5915-23. 2008
    ..This is the first report of quorum-sensing control of the acetate switch, and it indicates a metabolic connection between acetate utilization and cell density...
  12. pmc Vibrio fischeri uses two quorum-sensing systems for the regulation of early and late colonization factors
    Claudia Lupp
    Pacific Biomedical Research Center, University of Hawaii at Manoa, Honolulu, USA
    J Bacteriol 187:3620-9. 2005
    ..g., motility) by ain quorum sensing, whereas late colonization factors (e.g., luminescence) are preferentially regulated by lux quorum sensing...
  13. pmc Transcriptional patterns in both host and bacterium underlie a daily rhythm of anatomical and metabolic change in a beneficial symbiosis
    Andrew M Wier
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 107:2259-64. 2010
    ..Thus, the persistent maintenance of the squid-vibrio symbiosis is tied to a dynamic diel rhythm that involves both partners...
  14. pmc Vibrio fischeri LuxS and AinS: comparative study of two signal synthases
    Claudia Lupp
    Pacific Biomedical Research Center, University of Hawaii at Manoa, 41 Ahui St, Honolulu, HI 96813, USA
    J Bacteriol 186:3873-81. 2004
    ..Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation and colonization competence; however, its quantitative contribution is small when compared to that of the AinS signal...
  15. ncbi request reprint Confocal microscopy of the light organ crypts in juvenile Euprymna scolopes reveals their morphological complexity and dynamic function in symbiosis
    Laura K Sycuro
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Morphol 267:555-68. 2006
    ....
  16. pmc Recognition between symbiotic Vibrio fischeri and the haemocytes of Euprymna scolopes
    Spencer V Nyholm
    Kewalo Marine Laboratory, University of Hawaii, Honolulu, HI 96813, USA
    Environ Microbiol 11:483-93. 2009
    ..These data suggest that regulation of host haemocyte binding by the symbiont may be one of many factors that contribute to specificity in this association...
  17. pmc FlrA, a sigma54-dependent transcriptional activator in Vibrio fischeri, is required for motility and symbiotic light-organ colonization
    Deborah S Millikan
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    J Bacteriol 185:3547-57. 2003
    ..Thus, the transcriptional activator FlrA plays an essential role in regulating motility, and apparently in modulating other symbiotic functions, in V. fischeri...
  18. pmc GacA regulates symbiotic colonization traits of Vibrio fischeri and facilitates a beneficial association with an animal host
    Cheryl A Whistler
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    J Bacteriol 185:7202-12. 2003
    ..This study establishes the role of GacA as a regulator of a beneficial animal-microbe association and indicates that GacA regulates utilization of growth substrates as well as other colonization traits...
  19. pmc Population dynamics of Vibrio fischeri during infection of Euprymna scolopes
    Jessica McCann
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    Appl Environ Microbiol 69:5928-34. 2003
    ..Further, these results indicated that, when exposed to low numbers of V. fischeri, the host may become colonized by only one or a few bacterial cells, suggesting that symbiotic infection is highly efficient...
  20. pmc H-NOX-mediated nitric oxide sensing modulates symbiotic colonization by Vibrio fischeri
    Yanling Wang
    Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 107:8375-80. 2010
    ..Our data suggest that V. fischeri normally senses a host-generated NO signal through H-NOX(Vf) and modulates the expression of its iron uptake capacity during the early stages of the light-organ symbiosis...
  21. pmc Alterations in Vibrio fischeri motility correlate with a delay in symbiosis initiation and are associated with additional symbiotic colonization defects
    Deborah S Millikan
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    Appl Environ Microbiol 68:2519-28. 2002
    ..Furthermore, the pleiotropic nature of class III HS strains provides evidence that motility is coregulated with other symbiotic determinants in V. fischeri...
  22. ncbi request reprint The Vibrio fischeri sapABCDF locus is required for normal growth, both in culture and in symbiosis
    Claudia Lupp
    University of Hawaii, Kewalo Marine Laboratory, 41 Ahui Street, Honolulu, HI 96813, USA
    Arch Microbiol 179:57-65. 2002
    ..Thus, while the sap locus is important to the normal growth of V. fischeri, it plays different physiological roles in growth and tissue colonization than it does in enteric pathogens...
  23. pmc A novel lux operon in the cryptically bioluminescent fish pathogen Vibrio salmonicida is associated with virulence
    Eric J Nelson
    Department of Medical Microbiology and Immunology, 1300 University Ave, University of Wisconsin, Madison, WI 53706, USA
    Appl Environ Microbiol 73:1825-33. 2007
    ..Nevertheless, these findings document for the first time an association between a mutation in a structural lux gene and virulence, as well as provide a new molecular system to study Vibrio pathogenesis in a natural host...
  24. pmc Vibrio fischeri flagellin A is essential for normal motility and for symbiotic competence during initial squid light organ colonization
    Deborah S Millikan
    Pacific Biomedical Research Center, University of Hawaii, Honolulu, Hawaii 96813, USA
    J Bacteriol 186:4315-25. 2004
    ..fischeri is a complex structure consisting of multiple flagellin subunits, including FlaA, which is essential both for normal flagellation and for motility, as well as for effective symbiotic colonization...
  25. ncbi request reprint RP4-based plasmids for conjugation between Escherichia coli and members of the Vibrionaceae
    Eric V Stabb
    Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA
    Methods Enzymol 358:413-26. 2002
  26. ncbi request reprint Vibrio fischeri and its host: it takes two to tango
    Karen L Visick
    Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL 60153, USA
    Curr Opin Microbiol 9:632-8. 2006
    ..fischeri, an expressed sequence tagged library for E. scolopes and the availability of dual-fluorescence markers and confocal microscopy to probe symbiotic structures and the dynamics of colonization...
  27. ncbi request reprint The GacA global regulator of Vibrio fischeri is required for normal host tissue responses that limit subsequent bacterial colonization
    Cheryl A Whistler
    Department of Microbiology, University of New Hampshire, Durham, NH 03824 3587, USA
    Cell Microbiol 9:766-78. 2007
    ....
  28. pmc Two-component response regulators of Vibrio fischeri: identification, mutagenesis, and characterization
    Elizabeth A Hussa
    Department of Microbiology and Immunology, Loyola University Chicago, 2160 S First Avenue, Maywood, IL 60153, USA
    J Bacteriol 189:5825-38. 2007
    ..This work represents the first large-scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the importance of two-component signal transduction in bacterium-host interactions...
  29. pmc Transcriptome analysis of the Vibrio fischeri LuxR-LuxI regulon
    Luis Caetano M Antunes
    Department of Microbiology, University of Iowa, Iowa City, Iowa 52242, USA
    J Bacteriol 189:8387-91. 2007
    ..This demonstrates that V. fischeri quorum sensing regulates a substantial number of genes other than those involved in light production...
  30. pmc Characterization of htrB and msbB mutants of the light organ symbiont Vibrio fischeri
    Dawn M Adin
    University of Georgia, Department of Microbiology, 1000 Cedar Street, Athens, GA 30602, USA
    Appl Environ Microbiol 74:633-44. 2008
    ..7-fold during colonization when mixed with the parent. These data suggest that htrB2 and msbB play conserved general roles in vibrio biology, whereas htrB1 plays a more symbiosis-specific role in V. fischeri...

Research Grants17

  1. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2004
    ..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  2. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2009
    ..We will investigate a natural bacterial association to define the signaling events that underlie a symbiont-induced daily remodeling of the host's tissues that leads to a healthy persistent interaction. ..
  3. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2007
    ..The work proposed here will also provide new informationabout how specific, stable associationswith aquatic invertebrates may serve as environmentalreservoirs for human pathogenic Vibrio species. ..
  4. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2006
    ..The work proposed here will also provide new information about how specific, stable associations with aquatic invertebrates may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  5. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2006
    ..The work proposed here will also provide new information about how specific, stable associations with aquatic invertebrates may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  6. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2005
    ..The work proposed here will also provide new information about how specific, stable associations with aquatic invertebrates may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  7. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2003
    ..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  8. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2002
    ..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  9. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2001
    ..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..
  10. COLONIZATION OF EPITHELIUM BY SYMBIOTIC VIBRIO FISCHERI
    Edward Ruby; Fiscal Year: 1999
    ....
  11. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward G Ruby; Fiscal Year: 2010
    ..We will investigate a natural bacterial association to define the signaling events that underlie a symbiont-induced daily remodeling of the host's tissues that leads to a healthy persistent interaction. ..
  12. COLONIZATION OF EPITHELIUM BY SYMBIOTIC VIBRIO FISCHERI
    Edward Ruby; Fiscal Year: 1999
    ....
  13. VIBRIO VIRULENCE DETERMINANTS IN A BENIGN COLONIZATION
    Edward Ruby; Fiscal Year: 2000
    ..The work proposed here will also provide new information about the mechanisms by which specific, stable colonizations of clams, snails, squid and their relatives may serve as environmental reservoirs for human pathogenic Vibrio species. ..