Research Topics
Genomes and Genes | E RozengurtSummaryAffiliation: University of California Country: USA Publications
Research Grants
| Collaborators
|
Detail Information
Publications
Taste receptors in the gastrointestinal tract. I. Bitter taste receptors and alpha-gustducin in the mammalian gutEnrique Rozengurt
Division of Digestive Diseases and CURE Digestive Diseases Research Center, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, CA 90095 1786, USA
Am J Physiol Gastrointest Liver Physiol 291:G171-7. 2006....- Gastrin, CCK, signaling, and cancerE Rozengurt
Department of Medicine, School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
Annu Rev Physiol 63:49-76. 2001.... - Neuropeptides as growth factors for normal and cancerous cellsEnrique Rozengurt
Department Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Trends Endocrinol Metab 13:128-34. 2002..As our understanding of the role of neuropeptides in cancer increases, novel possibilities for translational research are emerging for improving the diagnosis and treatment of the disease... - Protein kinase D signalingEnrique Rozengurt
Department of Medicine, UCLA-CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
J Biol Chem 280:13205-8. 2005 - Colocalization of the alpha-subunit of gustducin with PYY and GLP-1 in L cells of human colonNora Rozengurt
Department of Pathology, David Geffen School of Medicine, University of California Los Angeles, 900 Veteran Ave, Warren Hall Rm 11 115, Los Angeles, CA 90095 1786, USA
Am J Physiol Gastrointest Liver Physiol 291:G792-802. 2006.... 
Rapid protein kinase D1 signaling promotes migration of intestinal epithelial cellsSteven H Young
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE, University of California at Los Angeles, Los Angeles, CA 90095 1786, USA
Am J Physiol Gastrointest Liver Physiol 303:G356-66. 2012..These results demonstrate that PKD1 activation is one of the early events initiated by wounding a monolayer of intestinal epithelial cells and indicate that PKD1 signaling promotes the migration of these cells in vitro and in vivo...
Protein kinase D signaling: multiple biological functions in health and diseaseEnrique Rozengurt
Department of Medicine, Division of Digestive Diseases, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California, USA
Physiology (Bethesda) 26:23-33. 2011....- Mitogenic signaling pathways induced by G protein-coupled receptorsEnrique Rozengurt
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095 1786, USA
J Cell Physiol 213:589-602. 2007..The purpose of this article is to review recent advances in defining the pathways that play a role in transducing mitogenic responses induced by GPCR agonists... - Taste receptor signaling in the mammalian gutEnrique Rozengurt
Division of Digestive Diseases and CURE, Digestive Diseases Research Center, David Geffen School of Medicine, University of California at Los Angeles, 900 Veteran Avenue, Los Angeles, CA 90095, USA
Curr Opin Pharmacol 7:557-62. 2007.... - Crosstalk between insulin/insulin-like growth factor-1 receptors and G protein-coupled receptor signaling systems: a novel target for the antidiabetic drug metformin in pancreatic cancerEnrique Rozengurt
Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, California 90095 1786, USA
Clin Cancer Res 16:2505-11. 2010..We posit that crosstalk between insulin/IGF-1 receptor and GPCR signaling is a mechanism for promoting the development of certain types of cancer and a target for the prevention and therapy of these diseases via metformin administration... - EGF receptor function is required in late G(1) for cell cycle progression induced by bombesin and bradykininC Santiskulvong
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095-1786, USA
Am J Physiol Cell Physiol 281:C886-98. 2001..Our results indicate that EGFR kinase activity is necessary in mid-late G(1) for promoting the accumulation of cyclins D1 and E and implicate EGFR function in the coupling of GPCR signaling to the activation of the cell cycle... - Rapid protein kinase D translocation in response to G protein-coupled receptor activation. Dependence on protein kinase CO Rey
Unit of Signal Transduction and Gastrointestinal Cancer, Department of Medicine, UCLA School of Medicine, Los Angeles, California 90095, USA
J Biol Chem 276:32616-26. 2001..These findings provide evidence for a novel mechanism by which PKC coordinates the translocation and activation of PKD in response to bombesin-induced GPCR activation... - Activation loop Ser744 and Ser748 in protein kinase D are transphosphorylated in vivoR T Waldron
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, and Molecular Biology Institute, UCLA School of Medicine and UCLA-CURE Digestive Diseases Research Center, Los Angeles, California 90095, USA
J Biol Chem 276:32606-15. 2001..Our results provide new experimental evidence indicating that activation loop phosphorylation at Ser(744) and Ser(748) occurs during PKD activation in vivo and support the notion of a PKC-PKD phosphorylation cascade... - Amino acid-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor are mediated by a phospholipase C/inositol 1,4,5-trisphosphate-independent pathway that requires G12, Rho, filamin-A, and the actin cytoskeletonOsvaldo Rey
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center, David Geffen School of Medicine, University of California at Los Angeles, 90095, USA
J Biol Chem 280:22875-82. 2005..Our model envisages that Ca(2+) or amino acids stabilize unique CaR conformations that favor coupling to different G proteins and subsequent activation of distinct downstream signaling pathways... - Activation of protein kinase D by signaling through Rho and the alpha subunit of the heterotrimeric G protein G13J Yuan
Department of Medicine, School of Medicine and Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
J Biol Chem 276:38619-27. 2001.... - Y-27632, an inhibitor of Rho-associated kinases, prevents tyrosine phosphorylation of focal adhesion kinase and paxillin induced by bombesin: dissociation from tyrosine phosphorylation of p130(CAS)J Sinnett-Smith
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, 90095-1786, USA
Exp Cell Res 266:292-302. 2001..The results demonstrate that ROCK-dependent tyrosine phosphorylation of FAK and paxillin can be dissociated from a ROCK-independent pathway leading to tyrosine phosphorylation of CAS... - Calyculin-A induces focal adhesion assembly and tyrosine phosphorylation of p125(Fak), p130(Cas), and paxillin in Swiss 3T3 cellsD Leopoldt
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
J Cell Physiol 188:106-19. 2001..Thus, calyculin-A induces transient focal adhesion assembly and tyrosine phosphorylation of p125(Fak), p130(Cas), and paxillin, acting downstream of ROK... - G protein-coupled receptor-mediated phosphorylation of the activation loop of protein kinase D: dependence on plasma membrane translocation and protein kinase CepsilonOsvaldo Rey
Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California Los Angeles, CA 90095, USA
J Biol Chem 279:34361-72. 2004..Based on these and previous results, we propose a model of GPCR-mediated PKD regulation that integrates its changes in distribution, catalytic activity, and multisite phosphorylation... - Vasopressin-mediated mitogenic signaling in intestinal epithelial cellsTerence Chiu
Department of Medicine, School of Medicine, University of California-Los Angeles, 900 Veteran Ave, Los Angeles, CA 90095, USA
Am J Physiol Cell Physiol 282:C434-50. 2002..Furthermore, AVP acts as a potent growth factor for IEC-18 cells, inducing DNA synthesis and cell proliferation through ERK-, Ca(2+)-, PKC-, EGFR tyrosine kinase-, and Src-dependent pathways... - Agonist-dependent immobilization of chimeric bombesin/GRP receptors: dependence on c-Src activity and dissociation from internalizationS H Young
Division of Digestive Diseases, University of California, Los Angeles, California 90095, USA
Exp Cell Res 267:37-44. 2001..These data suggest that agonist-bound GPCR have increased plasma membrane diffusion rates but an increased affinity for immobilization into a multiprotein complex that is mediated by Src activity... - Protein kinase D potentiates DNA synthesis and cell proliferation induced by bombesin, vasopressin, or phorbol esters in Swiss 3T3 cellsE Zhukova
Department of Medicine, School of Medicine and Molecular Biology Institute, UCLA, Los Angeles, CA 90095, USA
J Biol Chem 276:40298-305. 2001..Our results demonstrate that overexpression of PKD selectively potentiates mitogenesis induced by bombesin, vasopressin, or PDBu in Swiss 3T3 cells... - Galpha(13) stimulates Rho-dependent activation of the cyclooxygenase-2 promoterL W Slice
Division of Digestive Diseases, Department of Medicine, CURE, Digestive Diseases Research Center, University of California, Los Angeles, California 90095, USA
J Biol Chem 274:27562-6. 1999.... - Protein kinase D complexes with C-Jun N-terminal kinase via activation loop phosphorylation and phosphorylates the C-Jun N-terminusCliff Hurd
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California, CA 90095 1786, USA
Oncogene 21:2154-60. 2002.... - Neurotensin stimulates protein kinase C-dependent mitogenic signaling in human pancreatic carcinoma cell line PANC-1Sushovan Guha
Department of Medicine, Division of Digestive Diseases, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
Cancer Res 63:2379-87. 2003..Collectively our results suggest that PKC-mediated stimulation of ERK-1 and ERK-2 play a pivotal role in NT-induced growth of PANC-1 cells harboring activating K-Ras mutation... - PKD in intestinal epithelial cells: rapid activation by phorbol esters, LPA, and angiotensin through PKCT Chiu
Department of Medicine, UCLA School of Medicine and Molecular Biology Institute, Los Angeles, CA 90095, USA
Am J Physiol Cell Physiol 280:C929-42. 2001..PKD activation was tightly associated with an increase in PKD autophosphorylation at serine 916. Our results identify PKD as a novel early point of convergence and integration of G(i) and G(q) signaling in intestinal epithelial cells... - Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cellsS Vincent Wu
Department of Medicine, School of Medicine, Center for Ulcer Research and Education Digestive Diseases Research Center, Veterans Administration Greater Los Angeles Healthcare System, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 99:2392-7. 2002..These results demonstrate the expression of bitter taste receptors of the T2R family in the mouse and rat gastrointestinal tract... - Insulin potentiates Ca2+ signaling and phosphatidylinositol 4,5-bisphosphate hydrolysis induced by Gq protein-coupled receptor agonists through an mTOR-dependent pathwayKrisztina Kisfalvi
Unit of Signal Transduction and Gastrointestinal Cancer, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles Center for Ulcer Research and Education, Los Angeles, California 90095 1786, USA
Endocrinology 148:3246-57. 2007.... - Protein kinase D mediates mitogenic signaling by Gq-coupled receptors through protein kinase C-independent regulation of activation loop Ser744 and Ser748 phosphorylationJames Sinnett-Smith
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Biol Chem 284:13434-45. 2009..Thus, our results identify a novel mechanism of G(q)-coupled receptor-induced mitogenesis mediated by sustained PKD activation through a PKC-independent pathway... - ANG II stimulates PKC-dependent ERK activation, DNA synthesis, and cell division in intestinal epithelial cellsTerence Chiu
Department of Medicine, School of Medicine, CURE: Digestive Diseases Research Center and Molecular Biology Institute, University of California-Los Angeles, Los Angeles, CA 90095-1786, USA
Am J Physiol Gastrointest Liver Physiol 285:G1-11. 2003..Taken together, our results indicate that receptor-mediated PKC activation, as induced by ANG II, transduces mitogenic signals leading to DNA synthesis and cell proliferation in IEC-18 cells... - Induced overexpression of protein kinase D1 stimulates mitogenic signaling in human pancreatic carcinoma PANC-1 cellsKrisztina Kisfalvi
Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine, Molecular Biology Institute, University of California, Los Angeles, California 90095 1786, USA
J Cell Physiol 223:309-16. 2010..These results indicate that PKD1 mediates mitogenic signaling in PANC-1 and suggests that this enzyme could be a novel target for the development of therapeutic drugs that restrict the proliferation of these cells... - Protein kinase D3 activation and phosphorylation by signaling through G alpha qJingzhen Yuan
Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA
Biochem Biophys Res Commun 335:270-6. 2005..Our results indicate that G alpha q activation is sufficient to stimulate sustained PKD3 activation and show that the endogenous G alpha q is a major component in the signaling pathway that mediates bombesin-induced PKD3 activation... - G protein-coupled receptor signaling in human ductal pancreatic cancer cells: neurotensin responsiveness and mitogenic stimulationN M Ryder
Department of Surgery School of Medicine, University of California, Los Angeles 90095-1786, USA
J Cell Physiol 186:53-64. 2001..The signaling complexity of GPCRs uncovered by these studies reveals a new aspect in the biology of human pancreatic cancer and could offer the basis for new approaches to the treatment of this disease... - CCK2 (CCK(B)/gastrin) receptor mediates rapid protein kinase D (PKD) activation through a protein kinase C-dependent pathwayT Chiu
Department of Medicine, UCLA School of Medicine and Molecular Biology Institute, 900 Veteran Avenue, Warren Hall, Room 11-124, University of California, Los Angeles, CA 90095-1786, USA
FEBS Lett 489:101-6. 2001..Our results indicate that PKC-dependent PKD activation is a novel early event in the action of gastrin and CCK-8 via CCK2 receptors... - Differential FAK phosphorylation at Ser-910, Ser-843 and Tyr-397 induced by angiotensin II, LPA and EGF in intestinal epithelial cellsXiaohua Jiang
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095 178622, USA
Cell Signal 19:1000-10. 2007..Our results indicate that Ser-910 and Ser-843, rather than Tyr-397, are prominent sites differentially phosphorylated in response to neurotransmitters, bioactive lipids, tumor promoters and growth factors in intestinal epithelial cells... - PKD, PKD2, and p38 MAPK mediate Hsp27 serine-82 phosphorylation induced by neurotensin in pancreatic cancer PANC-1 cellsJingzhen Yuan
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Cell Biochem 103:648-62. 2008..Our results demonstrate, for the first time, that neurotensin induces a striking increase in Hsp27 phosphorylation on Ser-82 in PANC-1 cells through convergent p38 MAPK, PKD, and PKD2 signaling... - Activation of protein kinase D3 by signaling through Rac and the alpha subunits of the heterotrimeric G proteins G12 and G13Jingzhen Yuan
Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, 900 Veteran Ave, Warren Hall, Rm. 11-124, Los Angeles, CA 90095-1786, USA
Cell Signal 18:1051-62. 2006..Our results indicate that Galpha12/13 and Rac are important components in the signal transduction pathways that mediate bombesin receptor-induced PKD3 activation... - Protein kinase D is sufficient to suppress EGF-induced c-Jun Ser 63 phosphorylationC Hurd
Department of Medicine, University of California, Los Angeles, California 90095, USA
Biochem Biophys Res Commun 282:404-8. 2001..This is the first demonstration that PKD modulates phosphorylation of the proto-oncogene c-Jun at a site critical for its ability to mediate cell proliferation and differentiation... - Regulated nucleocytoplasmic transport of protein kinase D in response to G protein-coupled receptor activationO Rey
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, UCLA School of Medicine, Los Angeles, California 900095 1786, USA
J Biol Chem 276:49228-35. 2001..This study thus characterizes the regulated nuclear transport of a signaling molecule in response to mitogenic GPCR agonists and positions PKD as a serine kinase whose kinase activity and intracellular localization is coordinated by PKC... - Protein kinase D2 potentiates MEK/ERK/RSK signaling, c-Fos accumulation and DNA synthesis induced by bombesin in Swiss 3T3 cellsJames Sinnett-Smith
Division of Digestive Diseases, Department of Medicine, School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Cell Physiol 211:781-90. 2007.... - Metformin disrupts crosstalk between G protein-coupled receptor and insulin receptor signaling systems and inhibits pancreatic cancer growthKrisztina Kisfalvi
Departments of Medicine, CURE, Digestive Diseases Research Center, Molecular Biology Institute, University of California at Los Angeles, 90095 1786, USA
Cancer Res 69:6539-45. 2009..These results raise the possibility that metformin could be a potential candidate in novel treatment strategies for human pancreatic cancer... - Protein kinase D2 mediates lysophosphatidic acid-induced interleukin 8 production in nontransformed human colonic epithelial cells through NF-kappaBTerence T Chiu
Dept of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095 1786, USA
Am J Physiol Cell Physiol 292:C767-77. 2007..Our results demonstrate, for the first time, the involvement of a member of the PKD family in the production of IL-8, a potent proinflammatory chemokine, by epithelial cells... - Sequential protein kinase C (PKC)-dependent and PKC-independent protein kinase D catalytic activation via Gq-coupled receptors: differential regulation of activation loop Ser(744) and Ser(748) phosphorylationRodrigo Jacamo
Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California 90095, USA
J Biol Chem 283:12877-87. 2008..The present studies identify a novel mechanism induced by GPCR activation that leads to late, PKC-independent PKD activation... - Hyperosmotic stress induces rapid focal adhesion kinase phosphorylation at tyrosines 397 and 577. Role of Src family kinases and Rho family GTPasesJ Adrian Lunn
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, UCLA-CURE, Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Biol Chem 279:45266-78. 2004.... - ANG II and LPA induce Pyk2 tyrosine phosphorylation in intestinal epithelial cells: role of Ca2+, PKC, and Rho kinaseSteven S Wu
Department of Pediatrics, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095-1786, USA
Am J Physiol Cell Physiol 282:C1432-44. 2002..Together, these results show that ANG II and LPA rapidly induce Pyk2 tyrosine phosphorylation in intestinal epithelial cells via separate Ca2+-, PKC-, and Rho-mediated pathways... - Protein kinase C phosphorylates protein kinase D activation loop Ser744 and Ser748 and releases autoinhibition by the pleckstrin homology domainRichard T Waldron
Division of Digestive Diseases, Department of Medicine, The David Geffen School of Medicine at UCLA, Los Angeles, California 90095-1786, USA
J Biol Chem 278:154-63. 2003..These studies provide evidence of a direct PKCepsilon-PKD phosphorylation cascade and provide additional insight into the activation mechanism... - Galardin (GM 6001), a broad-spectrum matrix metalloproteinase inhibitor, blocks bombesin- and LPA-induced EGF receptor transactivation and DNA synthesis in rat-1 cellsChintda Santiskulvong
Department of Medicine, School of Medicine, University of California, Los Angeles, CA 90095-1786, USA
Exp Cell Res 290:437-46. 2003..Taken together, our results suggest that MMPs act as early as 5 min, and up to around 3 h, to mediate GPCR-induced EGFR transactivation, ERK activation, and stimulation of DNA synthesis... - Carbachol induces p70S6K1 activation through an ERK-dependent but Akt-independent pathway in human colonic epithelial cellsXiaohua Jiang
Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine and Molecular Biology Institute, University of California at Los Angeles, CA 90095, USA
Biochem Biophys Res Commun 387:521-4. 2009..These findings imply that GPCR activation induces p70S6K1 via ERK rather than through the canonical PI 3-kinase/Akt/TSC/mTORC1 pathway in T84 colon carcinoma cells... - CCK(B)/gastrin receptor mediates synergistic stimulation of DNA synthesis and cyclin D1, D3, and E expression in Swiss 3T3 cellsE Zhukova
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1786, USA
J Cell Physiol 189:291-305. 2001..Thus, the CCK(B)/gastrin receptor transfected into Swiss 3T3 cells provides a novel model system to elucidate mitogenic signal transduction pathways and cell cycle events activated via this receptor... - Signal transduction pathways in the mitogenic response to G protein-coupled neuropeptide receptor agonistsE Rozengurt
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles 90095 1786, USA
J Cell Physiol 177:507-17. 1998..The purpose of this article is to review recent advances in unraveling the pathways that play a role in transducing mitogenic and migratory responses induced by G protein-coupled neuropeptide receptor agonists... - Ca2+-stimulated Ca2+ oscillations produced by the Ca2+-sensing receptor require negative feedback by protein kinase CSteven H Young
Department of Medicine, Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, David Geffen School of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, UCLA, 90095 1786, USA
J Biol Chem 277:46871-6. 2002..These results support a model in which phosphorylation of the CaR at the inhibitory threonine 888 by PKC provides the negative feedback needed to cause [Ca(2+)](i) oscillations mediated by this receptor... - FAK phosphorylation at Ser-843 inhibits Tyr-397 phosphorylation, cell spreading and migrationRodrigo Jacamo
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095, USA
J Cell Physiol 210:436-44. 2007.... - Preferential phosphorylation of focal adhesion kinase tyrosine 861 is critical for mediating an anti-apoptotic response to hyperosmotic stressJ Adrian Lunn
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, UCLA CURE, Digestive Diseases Research Center and Molecular Biology Institute, UCLA, Los Angeles, California 90095
J Biol Chem 282:10370-9. 2007.... - Protein kinase D is a downstream target of protein kinase CthetaJingzhen Yuan
Unit of Signal Transduction and Gastrointestinal Cancer, Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA
Biochem Biophys Res Commun 291:444-52. 2002..The regulation of PKD by PKCtheta reveals a new pathway in the signaling network existing between multiple members of the PKC superfamily and PKD... - Protein kinase C nu/protein kinase D3 nuclear localization, catalytic activation, and intracellular redistribution in response to G protein-coupled receptor agonistsOsvaldo Rey
Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095 1786, USA
J Biol Chem 278:23773-85. 2003..Thus, this study demonstrates that the intracellular distribution of PKD isoenzymes are distinct, and suggests that their signaling properties are regulated by differential localization... - The RAS effector RIN1 directly competes with RAF and is regulated by 14-3-3 proteinsYing Wang
Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, California 90095, USA
Mol Cell Biol 22:916-26. 2002..These data suggest that the normal localization and function of RIN1, as well as its ability to compete with RAF, are regulated in part by 14-3-3 binding, which in turn is controlled by PKD phosphorylation... - Crosstalk between insulin receptor and G protein-coupled receptor signaling systems leads to Ca²+ oscillations in pancreatic cancer PANC-1 cellsSteven H Young
Division of Digestive Diseases, Department of Medicine, CURE Digestive Diseases Research Center, David Geffen School of Medicine and Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095 1786, USA
Biochem Biophys Res Commun 401:154-8. 2010.... - Interleukin-7 and transforming growth factor-beta play counter-regulatory roles in protein kinase C-delta-dependent control of fibroblast collagen synthesis in pulmonary fibrosisLing Zhang
Department of Pathology, David Geffen School of Medicine at UCLA and Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, CA 90095, USA
J Biol Chem 279:28315-9. 2004..Thus, PKC-delta may play a critical role in the pathogenesis of pulmonary fibrosis and may serve as a molecular target for therapeutic intervention to suppress fibrosis... - PDGF and FGF induce focal adhesion kinase (FAK) phosphorylation at Ser-910: dissociation from Tyr-397 phosphorylation and requirement for ERK activationIsabel Hunger-Glaser
Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, CURE: Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California, USA
J Cell Physiol 200:213-22. 2004..Our results indicate that FAK phosphorylation at Tyr-397 and FAK phosphorylation at Ser-910 are induced in response to PDGF stimulation through different signaling pathways, namely PI 3-kinase and ERK, respectively... - HLA class I signal transduction is dependent on Rho GTPase and ROKEric J Lepin
UCLA Immunogenetics Center, David Geffen School of Medicine, University of California, Los Angeles, 1000 Veteran Avenue, Los Angeles, CA 90095, USA
Biochem Biophys Res Commun 323:213-7. 2004..These results suggest that HLA class I-induced signaling in EC is dependent on Rho GTPase and ROK... - EGF receptor transactivation mediates ANG II-stimulated mitogenesis in intestinal epithelial cells through the PI3-kinase/Akt/mTOR/p70S6K1 signaling pathwayTerence Chiu
Department of Medicine, School of Medicine, CURE, Digestive Diseases Research Center, Molecular Biology Institute, University of California, Los Angeles, California, USA
Am J Physiol Gastrointest Liver Physiol 288:G182-94. 2005..Taken together, our results demonstrate that EGFR transactivation mediates ANG II-stimulated mitogenesis through the PI3-kinase/Akt/mTOR/p70S6K1 signaling pathway in IEC-18 cells... - Neurotensin and EGF induce synergistic stimulation of DNA synthesis by increasing the duration of ERK signaling in ductal pancreatic cancer cellsKrisztina Kisfalvi
Department of Medicine, David Geffen School of Medicine, CURE: Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095-178622, USA
J Cell Physiol 202:880-90. 2005..Collectively, our results suggest that the sustained phase of ERK signaling mediates the synergistic effects of NT and EGF on DNA synthesis in pancreatic cancer cells... - Prostaglandin E2 activates mitogen-activated protein kinase/Erk pathway signaling and cell proliferation in non-small cell lung cancer cells in an epidermal growth factor receptor-independent mannerKostyantyn Krysan
UCLA Lung Cancer Research Program of Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, California 90095-1690, USA
Cancer Res 65:6275-81. 2005..Importantly, these findings suggest that COX-2 overexpression may contribute to EGFR inhibitor resistance in NSCLC... - Src family kinases are required for integrin-mediated but not for G protein-coupled receptor stimulation of focal adhesion kinase autophosphorylation at Tyr-397E P Salazar
Department of Medicine, School of Medicine and Molecular Biology Institute, UCLA, Los Angeles, California 90095, USA
J Biol Chem 276:17788-95. 2001.... - Protein kinase D isozymes activation and localization during mitosisRomeo Papazyan
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California at Los Angeles, 90095 1786, USA
Exp Cell Res 314:3057-68. 2008..Thus, this study reveals a connection between the PKD isozymes and cell division, suggesting a novel role for this family of serine/threonine kinases... - Amino acid sensing by enteroendocrine STC-1 cells: role of the Na+-coupled neutral amino acid transporter 2Steven H Young
Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095 1786, USA
Am J Physiol Cell Physiol 298:C1401-13. 2010.... - Uncoupling of protein kinase D from suppression of EGF-dependent c-Jun phosphorylation in cancer cellsCliff Hurd
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine and Molecular Biology Institute, University of California, Los Angeles, CA 90095 1786, USA
Biochem Biophys Res Commun 302:800-4. 2003.... - Bombesin, lysophosphatidic acid, and epidermal growth factor rapidly stimulate focal adhesion kinase phosphorylation at Ser-910: requirement for ERK activationIsabel Hunger-Glaser
Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California 90095, USA
J Biol Chem 278:22631-43. 2003..Our results demonstrate, for the first time, that stimulation with bombesin, lysophosphatidic acid, PDB, or EGF induces phosphorylation of endogenous FAK at Ser-910 via an ERK-dependent pathway in Swiss 3T3 cells... - The pleckstrin homology domain of protein kinase D interacts preferentially with the eta isoform of protein kinase CR T Waldron
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095 1786, USA
J Biol Chem 274:9224-30. 1999..g. novel PKCs epsilon and eta, thereby revealing a previously undetected degree of specificity among protein-protein interactions mediated by PH domains... - RNA interference elucidates the role of focal adhesion kinase in HLA class I-mediated focal adhesion complex formation and proliferation in human endothelial cellsYi Ping Jin
Department of Pathology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
J Immunol 178:7911-22. 2007..This study shows that FAK plays a critical role in class I-induced cell proliferation, cell survival, and focal adhesion assembly in EC and may promote the development of transplant-associated vasculopathy... - Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cellsJingzhen Yuan
Veterans Affairs Greater Los Angeles Healthcare System, West Los Angeles VA Healthcare Center, Los Angeles, CA 90073, USA
Am J Physiol Gastrointest Liver Physiol 295:G1190-201. 2008..Our results identify PKD1 as a novel early convergent point for PKCdelta and epsilon in the signaling pathways mediating NF-kappaB activation in pancreatitis... - Extracellular calcium sensing receptor stimulation in human colonic epithelial cells induces intracellular calcium oscillations and proliferation inhibitionOsvaldo Rey
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, Department of Medicine, CURE, Digestive Diseases Research Center, Molecular Biology Institute and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California at Los Angeles, California 90095 1786, USA
J Cell Physiol 225:73-83. 2010..Thus, these results indicate that the CaR can function in two signaling modes in human colonic epithelial cells offering a potential link between gastrointestinal responses and food/nutrients uptake and metabolism... - Oxidative stress induces protein kinase C-mediated activation loop phosphorylation and nuclear redistribution of protein kinase DRichard T Waldron
Division of Digestive Diseases, Department of Medicine, UCLA David Geffen School of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, UCLA, Los Angeles, California 90095 1786, USA
J Biol Chem 279:27482-93. 2004..Taken together, these results indicate that PKC-mediated PKD Ser(744) and Ser(748) phosphorylation induced by oxidative stress integrates PKD activation with redistribution to the nucleus... - TNF-α potentiates lysophosphatidic acid-induced COX-2 expression via PKD in human colonic myofibroblastsCitlali Ekaterina Rodriguez Perez
Departments of Surgery and Medicine, David Geffen School of Medicine, CURE Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, 90095, USA
Am J Physiol Gastrointest Liver Physiol 300:G637-46. 2011..PKD plays a critical role in the expression of COX-2 in human colonic MFBs and may contribute to an inflammatory microenvironment that promotes tumor growth... - Vasopressin-induced intracellular redistribution of protein kinase D in intestinal epithelial cellsOsvaldo Rey
Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California at Los Angeles, California, USA
J Cell Physiol 196:483-92. 2003..These findings provide evidence for a mechanism by which PKC coordinates in intestinal epithelial cells the translocation and activation of PKD in response to vasopressin-induced GPCR activation... - Bombesin and angiotensin II rapidly stimulate Src phosphorylation at Tyr-418 in fibroblasts and intestinal epithelial cells through a PP2-insensitive pathwaySteven S Wu
Department of Pediatrics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Cell Signal 17:93-102. 2005..These results imply that a distinct, non-Src family kinase may be responsible for phosphorylating Src at Tyr-418 in intact fibroblasts and epithelial cells stimulated by GPCR agonists... - Intracellular redistribution of protein kinase D2 in response to G-protein-coupled receptor agonistsOsvaldo Rey
Division of Digestive Diseases, Department of Medicine, UCLA CURE Digestive Diseases Research Center and Molecular Biology Institute, David Geffen School of Medicine, University of California, Los Angeles, 90095 1786, USA
Biochem Biophys Res Commun 302:817-24. 2003.... - Dissociation of focal adhesion kinase and paxillin tyrosine phosphorylation induced by bombesin and lysophosphatidic acid from epidermal growth factor receptor transactivation in Swiss 3T3 cellsEduardo Perez Salazar
Department of Medicine, School of Medicine and Molecular Biology Institute, University of California, Los Angeles, California 90095 178622, USA
J Cell Physiol 194:314-24. 2003..Collectively, our results clearly dissociate EGFR transactivation from the tyrosine phosphorylation of FAK and paxillin induced by multiple GPCR agonists... - RNA interference reveals a differential role of FAK and Pyk2 in cell migration, leading edge formation and increase in focal adhesions induced by LPA in intestinal epithelial cellsXiaohua Jiang
Department of Medicine, Division of Digestive Diseases, David Geffen School of Medicine, CURE: Digestive Diseases Research Center and Molecular Biology Institute, University of California, Los Angeles, California 90095-178622, USA
J Cell Physiol 207:816-28. 2006.... - Secretin regulates paracellular permeability in canine gastric monolayers by a Src kinase-dependent pathwayMonica C Chen
CURE/Division of Digestive Diseases, Department of Medicine, School of Medicine, University of California Los Angeles and The Medical and Research Services, Greater Los Angeles Veterans Affairs Health Care System, Los Angeles, California 90073, USA
Am J Physiol Gastrointest Liver Physiol 283:G893-9. 2002..Src appears to mediate the physiological effects of this G(s)-coupled receptor in primary epithelial cells... - The lipid-associated 3D structure of SPA, a broad-spectrum neuropeptide antagonist with anticancer propertiesDavid A Keire
CURE Digestive Diseases Research Center, VA Greater Los Angeles Healthcare System, Los Angeles, CA 90073, USA
Biophys J 91:4478-89. 2006..These turns juxtapose the N- and C-termini of SPA and may form the basis of this peptide's unique ability to inhibit peptide receptor interactions at multiple receptor types... - Enteroendocrine cells: a site of 'taste' in gastrointestinal chemosensingCatia Sternini
Division of Digestive Diseases and CURE Digestive Diseases Research Center, and Departments of Medicine, USA
Curr Opin Endocrinol Diabetes Obes 15:73-8. 2008.... - Differential regulation of Pyk2 phosphorylation at Tyr-402 and Tyr-580 in intestinal epithelial cells: roles of calcium, Src, Rho kinase, and the cytoskeletonSteven S Wu
Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, 900 Veteran Avenue, Los Angeles, CA 90095, USA
Cell Signal 18:1932-40. 2006..This contrasts with the tight sequential coupling of phosphorylation seen in FAK activation, and further underlines the differences between these closely related kinases... - Protein kinase D isoforms are expressed in rat and mouse primary sensory neurons and are activated by agonists of protease-activated receptor 2Silvia Amadesi
Center for Neurobiology of Digestive Diseases, University of California, San Francisco, San Francisco, California 94143 0660, USA
J Comp Neurol 516:141-56. 2009..PKD may be a novel component of a signal transduction pathway for protease-induced activation of nociceptive neurons and an important new target for antiinflammatory and analgesic therapies... - Protein kinase D interacts with Golgi via its cysteine-rich domainO Rey
Unit of Signal Transduction and Gastrointestinal Cancer, Division of Digestive Diseases, UCLA School of Medicine, Los Angeles, CA 90095 1786, USA
Biochem Biophys Res Commun 287:21-6. 2001..The CRD of PKD also mediates its interaction with the plasma membrane, further supporting the conclusion that the CRD of PKD may act as a subcellular localization signal...
Research Grants
- GASTROINTESTINAL PEPTIDE SIGNALING THROUGH PKC/PKDJUAN ROZENGURT; Fiscal Year: 2007..abstract_text> ..
- GI PEPTIDE SIGNALING THROUGH TYROSINE PHOSPHORYLATIONJUAN ROZENGURT; Fiscal Year: 2007....
- GASTROINTESTINAL PEPTIDE SIGNALING THROUGH PKC/PKDJUAN ROZENGURT; Fiscal Year: 2009....
- GI PEPTIDE SIGNALING THROUGH TYROSINE PHOSPHORYLATIONJUAN ENRIQUE ROZENGURT; Fiscal Year: 2010....
- GASTROINTESTINAL PEPTIDE SIGNALING THROUGH PKC/PKDJUAN ENRIQUE ROZENGURT; Fiscal Year: 2010....
- GI PEPTIDE SIGNALING THROUGH TYROSINE PHOSPHORYLATIONJUAN ROZENGURT; Fiscal Year: 2004..2b) Determine the role of EGFR transactivation in signaling GI peptide-mediated mitogenesis. 2c) Characterize GPCR-induced transactivation of EGFR in IEC-6 cells. ..
- GASTROINTESTINAL PEPTIDE SIGNALING THROUGH PKC/PKDJUAN ROZENGURT; Fiscal Year: 2002....
- GASTROINTESTINAL PEPTIDE SIGNALING THROUGH PKC/PKDJUAN ENRIQUE ROZENGURT; Fiscal Year: 2010....