WILLIAM ROYER

Summary

Affiliation: University of Massachusetts Medical School
Country: USA

Publications

  1. pmc Structural hierarchy in erythrocruorin, the giant respiratory assemblage of annelids
    W E Royer
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 97:7107-11. 2000
  2. pmc Low resolution crystal structure of Arenicola erythrocruorin: influence of coiled coils on the architecture of a megadalton respiratory protein
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Mol Biol 365:226-36. 2007
  3. ncbi request reprint Lumbricus erythrocruorin at 3.5 A resolution: architecture of a megadalton respiratory complex
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Structure 14:1167-77. 2006
  4. ncbi request reprint Allosteric hemoglobin assembly: diversity and similarity
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 280:27477-80. 2005
  5. ncbi request reprint Cooperative hemoglobins: conserved fold, diverse quaternary assemblies and allosteric mechanisms
    W E Royer
    Dept of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Trends Biochem Sci 26:297-304. 2001
  6. ncbi request reprint Ligand-linked structural transitions in crystals of a cooperative dimeric hemoglobin
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Biochemistry 42:4640-7. 2003
  7. ncbi request reprint Crystal structure of the hemoglobin dodecamer from Lumbricus erythrocruorin: allosteric core of giant annelid respiratory complexes
    Kristen Strand
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, LRB 921, 364 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 344:119-34. 2004
  8. pmc Insights into interferon regulatory factor activation from the crystal structure of dimeric IRF5
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Struct Mol Biol 15:1213-20. 2008
  9. pmc Contribution of Ser386 and Ser396 to activation of interferon regulatory factor 3
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 379:251-60. 2008
  10. pmc Structural insights into interferon regulatory factor activation
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Cell Signal 22:883-7. 2010

Research Grants

Collaborators

Detail Information

Publications21

  1. pmc Structural hierarchy in erythrocruorin, the giant respiratory assemblage of annelids
    W E Royer
    Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 97:7107-11. 2000
    ..The resulting complex of linker chains forms a scaffold on which twelve hemoglobin dodecamers assemble. This structure specifies the unique, self-limited assemblage of a highly cooperative single molecule...
  2. pmc Low resolution crystal structure of Arenicola erythrocruorin: influence of coiled coils on the architecture of a megadalton respiratory protein
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Mol Biol 365:226-36. 2007
    ....
  3. ncbi request reprint Lumbricus erythrocruorin at 3.5 A resolution: architecture of a megadalton respiratory complex
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Structure 14:1167-77. 2006
    ..The observed diversity includes design features that have been incorporated into the linker subunits and may be critical for efficient assembly of large quantities of this complex respiratory protein...
  4. ncbi request reprint Allosteric hemoglobin assembly: diversity and similarity
    William E Royer
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 280:27477-80. 2005
  5. ncbi request reprint Cooperative hemoglobins: conserved fold, diverse quaternary assemblies and allosteric mechanisms
    W E Royer
    Dept of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA
    Trends Biochem Sci 26:297-304. 2001
    ..This mechanistic diversity, which occurs between assemblies of subunits that have the same fold, provides insight into the range of regulatory strategies that are available to protein molecules...
  6. ncbi request reprint Ligand-linked structural transitions in crystals of a cooperative dimeric hemoglobin
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    Biochemistry 42:4640-7. 2003
    ..These results show both the effects of a crystal lattice in limiting quaternary structural transitions and provide the first example of complete allosteric transitions within another crystal lattice...
  7. ncbi request reprint Crystal structure of the hemoglobin dodecamer from Lumbricus erythrocruorin: allosteric core of giant annelid respiratory complexes
    Kristen Strand
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, LRB 921, 364 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 344:119-34. 2004
    ..Thus, these results provide the first step towards elucidating the structural basis for the strong allosteric properties of Lumbricus erythrocruorin...
  8. pmc Insights into interferon regulatory factor activation from the crystal structure of dimeric IRF5
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, 364 Plantation Street, Worcester, Massachusetts 01605, USA
    Nat Struct Mol Biol 15:1213-20. 2008
    ..Thus, phosphorylation is likely to activate IRF5 and other family members by triggering conformational rearrangements that switch the C-terminal segment from an autoinihibitory to a dimerization role...
  9. pmc Contribution of Ser386 and Ser396 to activation of interferon regulatory factor 3
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    J Mol Biol 379:251-60. 2008
    ..These results indicate that Ser386 and Ser396 are critical for IRF-3 activation, and support a phosphorylation-oligomerization model for IRF-3 activation...
  10. pmc Structural insights into interferon regulatory factor activation
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605, USA
    Cell Signal 22:883-7. 2010
    ..The advances made in understanding the release of inhibition after IRF dimerization have generated a detailed structural model of how IRFs signaling pathways are activated...
  11. ncbi request reprint Competition between Ski and CREB-binding protein for binding to Smad proteins in transforming growth factor-beta signaling
    Weijun Chen
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA
    J Biol Chem 282:11365-76. 2007
    ..Our results suggest that Ski negatively regulates TGF-beta signaling by replacing CBP in R-Smad complexes. Our working model suggests that Smad protein activity is delicately balanced by Ski and CBP in the TGF-beta pathway...
  12. ncbi request reprint Residue F4 plays a key role in modulating oxygen affinity and cooperativity in Scapharca dimeric hemoglobin
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Biochemistry 44:14419-30. 2005
    ..These results highlight very different strategies used by cooperative hemoglobins in molluscs and mammals to control ligand affinity by modulation of the stereochemistry on the proximal side of the heme...
  13. pmc Allosteric action in real time: time-resolved crystallographic studies of a cooperative dimeric hemoglobin
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    Proc Natl Acad Sci U S A 103:7649-54. 2006
    ..Reversal of these changes shows a considerable lag as a T-like structure persists well after ligand rebinding, suggesting a slow T-to-R transition...
  14. pmc Ligand migration and cavities within Scapharca Dimeric HbI: studies by time-resolved crystallo-graphy, Xe binding, and computational analysis
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, The University of Massachusetts Medical School, Worcester, MA 01605, USA
    Structure 17:1494-504. 2009
    ..Taken together, these results are consistent with a distal histidine gate as one important ligand entry and exit route, despite its participation in the dimeric interface...
  15. ncbi request reprint Immobilization of Scapharca HbI crystals improves data quality in time-resolved crystallographic experiments
    James E Knapp
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Micron 35:107-8. 2004
    ..This leads to an increase in the signal to noise present in the electron density maps...
  16. pmc An optical signal correlated with the allosteric transition in Scapharca inaequivalvis HbI
    Jeffry C Nichols
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA
    Biochemistry 45:15748-55. 2006
    ..Unique values of allosteric parameters for several mutants have been obtained by combining kinetic and equilibrium data. The effect of mutation on function thus may be assigned to allostery or to a change in intrinsic heme reactivity...
  17. pmc Crystal structure of the APOBEC3G catalytic domain reveals potential oligomerization interfaces
    Shivender M D Shandilya
    Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    Structure 18:28-38. 2010
    ..NMR solution data provides evidence that another interface, which coordinates a novel zinc site, also exists. Thus, the observed crystallographic interfaces of APOBEC3G may be important for both oligomerization and function...
  18. pmc Time-resolved x-ray crystallography of heme proteins
    Vukica Srajer
    Center for Advanced Radiation Sources, The University of Chicago, Chicago, Illinois, USA
    Methods Enzymol 437:379-95. 2008
    ..These techniques have recently permitted observing for the first time allosteric transitions in real time for a cooperative dimeric hemoglobin...
  19. pmc Ligand migration and binding in the dimeric hemoglobin of Scapharca inaequivalvis
    Karin Nienhaus
    Institute of Biophysics, University of Ulm, 89069 Ulm, Germany
    Biochemistry 46:14018-31. 2007
    ..For HbI, the so-called histidine gate mechanism proposed for other globins appears as a plausible entry/exit route as well...
  20. ncbi request reprint Catalytic implications from the Drosophila protein L-isoaspartyl methyltransferase structure and site-directed mutagenesis
    Eric J Bennett
    Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, USA
    Biochemistry 42:12844-53. 2003
    ..Catalysis by dPIMT requires the side chain of the conserved, active site residue Ser-60, since substitution of this residue with Thr, Gln, or Ala reduces or abolishes the methylation of both protein and isoaspartyl peptide substrates...
  21. pmc Sulfide binding is mediated by zinc ions discovered in the crystal structure of a hydrothermal vent tubeworm hemoglobin
    Jason F Flores
    Department of Biology, Pennsylvania State University, University Park, PA 16802, USA
    Proc Natl Acad Sci U S A 102:2713-8. 2005
    ..pachyptila hemoglobins by addition of a thiol inhibitor were also unsuccessful. These findings challenge the currently accepted paradigm of annelid hemoglobin evolution and adaptation to reducing environments...

Research Grants21

  1. STRUCTURAL BASIS FOR ASSEMBLY OF COOPERATIVE HEMOGLOBINS
    WILLIAM ROYER; Fiscal Year: 2005
    ..Our structural analysis suggests a number of hypotheses for the regulation of function, which we will be testing by mutagenesis, kinetic and structural experiments. ..
  2. Ultrafast Time-Resolved Crystallography on Scapharca Hb
    WILLIAM ROYER; Fiscal Year: 2007
    ..Understanding such principles will aid in the rational manipulation of a number of biological processes. ..
  3. STRUCTURAL BASIS FOR ASSEMBLY OF COOPERATIVE HEMOGLOBINS
    WILLIAM ROYER; Fiscal Year: 2001
    ....
  4. STRUCTURAL BASIS FOR COOPERATIVITY IN CLAM HEMOGLOBINS
    WILLIAM ROYER; Fiscal Year: 1993
    ..Crystallization of a 43OkDa hemoglobin from a related arcid clam, Barbatia reeveana, will be pursued to determine the manner by which a tandem repeat of globin folds can be accommodated while still maintaining cooperative oxygen binding...
  5. Structural basis of regulation of IRF
    WILLIAM ROYER; Fiscal Year: 2007
    ..These studies will provide insight into the molecular mechanism of IRF signaling, providing possible targets for rational drug design to combat viral pathogenesis. ..