Wilma Ross

Summary

Affiliation: University of Wisconsin
Country: USA

Publications

  1. pmc Analysis of RNA polymerase-promoter complex formation
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, USA
    Methods 47:13-24. 2009
  2. pmc Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase
    Steven T Rutherford
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    J Mol Biol 366:1243-57. 2007
  3. pmc Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Mol Biol 377:551-64. 2008
  4. ncbi request reprint rRNA promoter regulation by nonoptimal binding of sigma region 1.2: an additional recognition element for RNA polymerase
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, 53706, USA
    Cell 125:1069-82. 2006
  5. pmc Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 19:2378-87. 2005
  6. ncbi request reprint rRNA transcription in Escherichia coli
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Annu Rev Genet 38:749-70. 2004
  7. pmc The magic spot: a ppGpp binding site on E. coli RNA polymerase responsible for regulation of transcription initiation
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    Mol Cell 50:420-9. 2013
  8. pmc Direct interactions between the coiled-coil tip of DksA and the trigger loop of RNA polymerase mediate transcriptional regulation
    Christopher W Lennon
    Department of Bacteriology, University of Wisconsin at Madison, Madison, WI 53706, USA
    Genes Dev 26:2634-46. 2012
  9. pmc Escherichia coli DksA binds to Free RNA polymerase with higher affinity than to RNA polymerase in an open complex
    Christopher W Lennon
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 191:5854-8. 2009
  10. pmc Fine structure of the promoter-sigma region 1.2 interaction
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:3292-7. 2008

Collaborators

Detail Information

Publications21

  1. pmc Analysis of RNA polymerase-promoter complex formation
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Dr, Madison, WI 53706, USA
    Methods 47:13-24. 2009
    ..Utilization of these methods can result in determination of not only basal promoter strength but also the rates of transcription initiation complex formation and decay...
  2. pmc Effects of DksA, GreA, and GreB on transcription initiation: insights into the mechanisms of factors that bind in the secondary channel of RNA polymerase
    Steven T Rutherford
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, Madison, WI 53706, USA
    J Mol Biol 366:1243-57. 2007
    ..Our results provide important clues to the mechanisms of both negative and positive control of transcription initiation by DksA...
  3. pmc Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Mol Biol 377:551-64. 2008
    ..coli ribosomal RNA transcription initiation and highlight the importance of inclusion of omega in bacterial RNAP preparations...
  4. ncbi request reprint rRNA promoter regulation by nonoptimal binding of sigma region 1.2: an additional recognition element for RNA polymerase
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin Madison, 420 Henry Mall, 53706, USA
    Cell 125:1069-82. 2006
    ..2-discriminator interaction, rRNA promoters create the short-lived complex required for specific responses to the RNAP binding factors ppGpp and DksA, ultimately accounting for regulation of ribosome synthesis...
  5. pmc Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA
    Catherine E Vrentas
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 19:2378-87. 2005
    ..Furthermore, these results suggest that either one or both of two short segments in the beta' subunit that physically link omega to the ppGpp-binding region of the enzyme may play crucial roles in ppGpp and DksA function...
  6. ncbi request reprint rRNA transcription in Escherichia coli
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    Annu Rev Genet 38:749-70. 2004
    ..Together, these advances provide clues important for our molecular understanding not only of rRNA transcription, but also of transcription in general...
  7. pmc The magic spot: a ppGpp binding site on E. coli RNA polymerase responsible for regulation of transcription initiation
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    Mol Cell 50:420-9. 2013
    ..Identification of the binding site allows prediction of bacterial species in which ppGpp exerts its effects by targeting RNAP...
  8. pmc Direct interactions between the coiled-coil tip of DksA and the trigger loop of RNA polymerase mediate transcriptional regulation
    Christopher W Lennon
    Department of Bacteriology, University of Wisconsin at Madison, Madison, WI 53706, USA
    Genes Dev 26:2634-46. 2012
    ..We suggest that DksA traps the TL in a conformation that destabilizes promoter complexes, an interaction explaining the requirement for the DksA tip and its effects on transcription...
  9. pmc Escherichia coli DksA binds to Free RNA polymerase with higher affinity than to RNA polymerase in an open complex
    Christopher W Lennon
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, WI 53706, USA
    J Bacteriol 191:5854-8. 2009
    ..These results suggest that the conformation of RNAP present in an open complex is not optimal for DksA binding and that DNA directly or indirectly alters the interface between the two proteins...
  10. pmc Fine structure of the promoter-sigma region 1.2 interaction
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 105:3292-7. 2008
    ..2. Our results refine models of RNAP-DNA interactions in the promoter complex that are crucial for regulation of transcription initiation...
  11. ncbi request reprint Binding modes of the initiator and inhibitor forms of the replication protein pi to the gamma ori iteron of plasmid R6K
    Selvi Kunnimalaiyaan
    Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706, USA
    J Biol Chem 279:41058-66. 2004
    ..These results support a model in which Rep protein dimerization disturbs one of two DNA binding domains important for monomer/iteron interaction; the dimer/iteron interaction utilizes only one DNA binding domain...
  12. pmc Allosteric control of Escherichia coli rRNA promoter complexes by DksA
    Steven T Rutherford
    Department of Bacteriology, University of Wisconsin at Madison, Madison, Wisconsin 53706, USA
    Genes Dev 23:236-48. 2009
    ..DksA thus inhibits the transition to RP(I). Our results illustrate in mechanistic terms how transcription factors can regulate initiation promoter-specifically without interacting directly with DNA...
  13. pmc Advances in bacterial promoter recognition and its control by factors that do not bind DNA
    Shanil P Haugen
    Department of Bacteriology, University of Wisconsin Madison, 1550 Linden Drive, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 6:507-19. 2008
    ..These factors can target promoters by affecting specific kinetic steps on the pathway to open complex formation, thereby regulating RNA output from specific promoters...
  14. pmc Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA
    Justin J Lemke
    Department of Bacteriology, University of Wisconsin, Madison, WI 53719, USA
    Proc Natl Acad Sci U S A 108:5712-7. 2011
    ..We conclude that direct control of r-protein promoters and rRNA promoters by the same signal, ppGpp/DksA, makes a major contribution to the balanced and coordinated synthesis rates of all of the ribosomal components...
  15. pmc General pathway for turning on promoters transcribed by RNA polymerases containing alternative sigma factors
    Richard L Gourse
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    J Bacteriol 188:4589-91. 2006
  16. pmc Role of the coiled-coil tip of Escherichia coli DksA in promoter control
    Jeong Hyun Lee
    Department of Bacteriology, University of Wisconsin Madison, Madison, WI 53706 1521, USA
    J Mol Biol 416:503-17. 2012
    ..That a single methylene group has such an extraordinary effect on these transcription factors highlights the critical nature of the identity of coiled-coil tip interactions with RNAP for open complex formation...
  17. pmc An intersubunit contact stimulating transcription initiation by E coli RNA polymerase: interaction of the alpha C-terminal domain and sigma region 4
    Wilma Ross
    Department of Bacteriology, University of Wisconsin Madison, Madison, Wisconsin 53706, USA
    Genes Dev 17:1293-307. 2003
    ..Previous data from the literature suggest that this same sigma-alphaCTD interaction also plays a role in transcription factor-mediated activation...
  18. ncbi request reprint DksA: a critical component of the transcription initiation machinery that potentiates the regulation of rRNA promoters by ppGpp and the initiating NTP
    Brian J Paul
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison 53706, USA
    Cell 118:311-22. 2004
    ....
  19. pmc Sequence-independent upstream DNA-alphaCTD interactions strongly stimulate Escherichia coli RNA polymerase-lacUV5 promoter association
    Wilma Ross
    Department of Bacteriology, University of Wisconsin, 420 Henry Mall, Madison, WI 53706, USA
    Proc Natl Acad Sci U S A 102:291-6. 2005
    ....
  20. ncbi request reprint Architecture of Fis-activated transcription complexes at the Escherichia coli rrnB P1 and rrnE P1 promoters
    Sarah E Aiyar
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706 1567, USA
    J Mol Biol 316:501-16. 2002
    ..Thus, similar Fis-alphaCTD interactions are used for activation of transcription when the activator is bound at very different positions on the DNA...
  21. ncbi request reprint Control of rRNA expression in Escherichia coli
    David A Schneider
    Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA
    Curr Opin Microbiol 6:151-6. 2003
    ..Recent work has dissected many of the molecular mechanisms responsible for the strength and regulation of rRNA promoters...