Research Topics
Genomes and Genes | Elliott M RossSummaryAffiliation: University of Texas Southwestern Medical Center Country: USA Publications
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Publications
Galpha(q) and phospholipase C-beta: turn on, turn off, and do it fastElliott M Ross
Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
Sci Signal 4:pe5. 2011..This cycle allows rapid sampling of the activation state of G(q)-coupled receptors while providing efficient signal transduction...
GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteinsE M Ross
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
Annu Rev Biochem 69:795-827. 2000..GAPs are regulated by various controls of their cellular concentrations, by complex interactions with G¿ or with G¿5 through an endogenous G-like domain, and by interaction with multiple other proteins...
Coordinating speed and amplitude in G-protein signalingElliott M Ross
Department of Pharmacology, Graduate Programs in Molecular Biophysics and Cell Regulation, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390 9041, USA
Curr Biol 18:R777-R783. 2008..Here, the principles of such coordination and the mechanisms by which it is achieved are discussed...- Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptorWei Tang
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
J Biol Chem 281:4746-53. 2006.... - Coordinate regulation of G protein signaling via dynamic interactions of receptor and GAPMARC TURCOTTE
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
PLoS Comput Biol 4:e1000148. 2008..The work also demonstrates the applicability of appropriately data-constrained system-level analysis to signaling networks of this scale... - Synergistic activation of phospholipase C-beta3 by Galpha(q) and Gbetagamma describes a simple two-state coincidence detectorFinly Philip
Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
Curr Biol 20:1327-35. 2010..Such synergism is commonly assumed to be complex, requiring regulatory interaction between components, multiple pathways, or multiple states of the target protein... - Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin IYaping Tu
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
J Biol Chem 278:52273-81. 2003..Synapsin can thus act as a phosphorylation-modulated mediator of feedback regulation of Gz signaling by the synaptic machinery... - RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamilyJ Wang
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
J Biol Chem 273:26014-25. 1998..Phosphorylation of Galphaz by protein kinase C inhibited the GAP activity of RGSZ1 and other RGS proteins, providing a mechanism for potentiation of Gz signaling by protein kinase C... - Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunitCelestine J Thomas
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9050, USA
Proc Natl Acad Sci U S A 101:7560-5. 2004..In (K180P)G alpha(i1), the two events are decoupled kinetically, whereas in the native protein they are concerted... - PDZ domain proteins: scaffolds for signaling complexesR Ranganathan
Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235 9041, USA
Curr Biol 7:R770-3. 1997..Extensive conservation of PDZ domains suggests that these motifs have a general role in organizing diverse signaling complexes... - Activation biosensor for G protein-coupled receptors: a FRET-based m1 muscarinic activation sensor that regulates G(q)Seungwoo Chang
Department of Pharmacology, Molecular Biophysics Graduate Program, Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA
PLoS ONE 7:e45651. 2012..The approach should be generally applicable to other Class I receptors which include numerous important drug targets... - Quantitative assays for GTPase-activating proteinsElliott M Ross
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Methods Enzymol 344:601-17. 2002 - Modulation of the affinity and selectivity of RGS protein interaction with G alpha subunits by a conserved asparagine/serine residueB A Posner
Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9041, USA
Biochemistry 38:7773-9. 1999..Detection of GAP activities of the mutants was enhanced in sensitivity up to 100-fold by assay at steady state in proteoliposomes that contain heterotrimeric G protein and receptor... - Inhibition of brain Gz GAP and other RGS proteins by palmitoylation of G protein alpha subunitsY Tu
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9041, USA
Science 278:1132-5. 1997..Reversible palmitoylation is thus a major determinant of Gz deactivation after its stimulation by receptors, and may be a general mechanism for prolonging or potentiating G-protein signaling... - WNK1 promotes PIP₂ synthesis to coordinate growth factor and GPCR-Gq signalingSung Wan An
Department of Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Curr Biol 21:1979-87. 2011..WNK1 protein kinase is known to regulate ion homeostasis and cause hypertension when expression is increased by gene mutations. However, its signaling functions remain largely elusive... - Spinophilin regulates Ca2+ signalling by binding the N-terminal domain of RGS2 and the third intracellular loop of G-protein-coupled receptorsXinhua Wang
Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
Nat Cell Biol 7:405-11. 2005..These findings provide a general mechanism by which RGS proteins recognize GPCRs to confer signalling specificity... - Palmitoylation of a conserved cysteine in the regulator of G protein signaling (RGS) domain modulates the GTPase-activating activity of RGS4 and RGS10Y Tu
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
J Biol Chem 274:38260-7. 1999..Dual palmitoylation of wild-type RGS4 remained inhibitory. RGS protein palmitoylation is thus multi-site, complex in its control, and either inhibitory or stimulatory depending on the RGS protein and its sites of palmitoylation... - Phospholipase C-beta1 directly accelerates GTP hydrolysis by Galphaq and acceleration is inhibited by Gbeta gamma subunitsP Chidiac
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
J Biol Chem 274:19639-43. 1999.... - Mammalian phospholipase CGanesh Kadamur
Department of Pharmacology, Molecular Biophysics Graduate Program and Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
Annu Rev Physiol 75:127-54. 2013.... - Mutations in the carboxyl-terminal domain of phospholipase C-beta 1 delineate the dimer interface and a potential Galphaq interaction siteOlga Ilkaeva
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041
J Biol Chem 277:4294-300. 2002..Its location in the molecule suggests that moving the attachment point of the catalytic domain can disrupt its ability to be activated by Galpha(q)... - RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20S A Barker
Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390 9041, USA
Genomics 78:223-9. 2001..At least two mRNAs that include the exon that encodes the N-terminal region unique to RGSZ1 were found in brain and a few other tissues, but not retina. RGS20 thus can account for multiple G(z)-selective GAPs in different tissues... - Binding of regulator of G protein signaling (RGS) proteins to phospholipid bilayers. Contribution of location and/or orientation to Gtpase-activating protein activityY Tu
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
J Biol Chem 276:20160-6. 2001..The RGS4 N-terminal domain confers similar membrane binding behavior on the RGS domains of either RGS10 or RGSZ1... - Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11, its physiologic regulatorG Berstein
Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235 9041
Cell 70:411-8. 1992..Such GAP activity by an effector coupled to a trimeric G protein can reconcile slow GTP hydrolysis by pure G proteins in vitro with fast physiologic deactivation of G protein-mediated signaling... - Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cellsE M Parker
Department of Pharmacology, Southwestern Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas 75235 9041
J Biol Chem 266:519-27. 1991..The beta-AR and m2 MAChR were characteristically stimulated by reduction of disulfides. These results demonstrate the general utility of the baculovirus system for production of large quantities of native G protein-coupled receptors...