Elliott M Ross

Summary

Affiliation: University of Texas Southwestern Medical Center
Country: USA

Publications

  1. pmc Activation biosensor for G protein-coupled receptors: a FRET-based m1 muscarinic activation sensor that regulates G(q)
    Seungwoo Chang
    Department of Pharmacology, Molecular Biophysics Graduate Program, Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS ONE 7:e45651. 2012
  2. doi request reprint Galpha(q) and phospholipase C-beta: turn on, turn off, and do it fast
    Elliott M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Sci Signal 4:pe5. 2011
  3. pmc Coordinating speed and amplitude in G-protein signaling
    Elliott M Ross
    Department of Pharmacology, Graduate Programs in Molecular Biophysics and Cell Regulation, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390 9041, USA
    Curr Biol 18:R777-R783. 2008
  4. ncbi request reprint GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins
    E M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Annu Rev Biochem 69:795-827. 2000
  5. ncbi request reprint Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptor
    Wei Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 281:4746-53. 2006
  6. pmc Coordinate regulation of G protein signaling via dynamic interactions of receptor and GAP
    MARC TURCOTTE
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS Comput Biol 4:e1000148. 2008
  7. pmc Synergistic activation of phospholipase C-beta3 by Galpha(q) and Gbetagamma describes a simple two-state coincidence detector
    Finly Philip
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Curr Biol 20:1327-35. 2010
  8. ncbi request reprint Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin I
    Yaping Tu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 278:52273-81. 2003
  9. ncbi request reprint RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamily
    J Wang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 273:26014-25. 1998
  10. pmc Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit
    Celestine J Thomas
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 101:7560-5. 2004

Collaborators

Detail Information

Publications25

  1. pmc Activation biosensor for G protein-coupled receptors: a FRET-based m1 muscarinic activation sensor that regulates G(q)
    Seungwoo Chang
    Department of Pharmacology, Molecular Biophysics Graduate Program, Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA
    PLoS ONE 7:e45651. 2012
    ..The approach should be generally applicable to other Class I receptors which include numerous important drug targets...
  2. doi request reprint Galpha(q) and phospholipase C-beta: turn on, turn off, and do it fast
    Elliott M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Sci Signal 4:pe5. 2011
    ..This cycle allows rapid sampling of the activation state of G(q)-coupled receptors while providing efficient signal transduction...
  3. pmc Coordinating speed and amplitude in G-protein signaling
    Elliott M Ross
    Department of Pharmacology, Graduate Programs in Molecular Biophysics and Cell Regulation, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, Texas 75390 9041, USA
    Curr Biol 18:R777-R783. 2008
    ..Here, the principles of such coordination and the mechanisms by which it is achieved are discussed...
  4. ncbi request reprint GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins
    E M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    Annu Rev Biochem 69:795-827. 2000
    ..GAPs are regulated by various controls of their cellular concentrations, by complex interactions with G¿ or with G¿5 through an endogenous G-like domain, and by interaction with multiple other proteins...
  5. ncbi request reprint Gbetagamma inhibits Galpha GTPase-activating proteins by inhibition of Galpha-GTP binding during stimulation by receptor
    Wei Tang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 281:4746-53. 2006
    ....
  6. pmc Coordinate regulation of G protein signaling via dynamic interactions of receptor and GAP
    MARC TURCOTTE
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas, USA
    PLoS Comput Biol 4:e1000148. 2008
    ..The work also demonstrates the applicability of appropriately data-constrained system-level analysis to signaling networks of this scale...
  7. pmc Synergistic activation of phospholipase C-beta3 by Galpha(q) and Gbetagamma describes a simple two-state coincidence detector
    Finly Philip
    Department of Pharmacology, University of Texas Southwestern Medical Center, 6001 Forest Park Road, Dallas, TX 75390 9041, USA
    Curr Biol 20:1327-35. 2010
    ..Such synergism is commonly assumed to be complex, requiring regulatory interaction between components, multiple pathways, or multiple states of the target protein...
  8. ncbi request reprint Phosphorylation-regulated inhibition of the Gz GTPase-activating protein activity of RGS proteins by synapsin I
    Yaping Tu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 278:52273-81. 2003
    ..Synapsin can thus act as a phosphorylation-modulated mediator of feedback regulation of Gz signaling by the synaptic machinery...
  9. ncbi request reprint RGSZ1, a Gz-selective RGS protein in brain. Structure, membrane association, regulation by Galphaz phosphorylation, and relationship to a Gz gtpase-activating protein subfamily
    J Wang
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 273:26014-25. 1998
    ..Phosphorylation of Galphaz by protein kinase C inhibited the GAP activity of RGSZ1 and other RGS proteins, providing a mechanism for potentiation of Gz signaling by protein kinase C...
  10. pmc Uncoupling conformational change from GTP hydrolysis in a heterotrimeric G protein alpha-subunit
    Celestine J Thomas
    Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390 9050, USA
    Proc Natl Acad Sci U S A 101:7560-5. 2004
    ..In (K180P)G alpha(i1), the two events are decoupled kinetically, whereas in the native protein they are concerted...
  11. ncbi request reprint PDZ domain proteins: scaffolds for signaling complexes
    R Ranganathan
    Howard Hughes Medical Institute, Department of Pharmacology, The University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, Texas 75235 9041, USA
    Curr Biol 7:R770-3. 1997
    ..Extensive conservation of PDZ domains suggests that these motifs have a general role in organizing diverse signaling complexes...
  12. ncbi request reprint Spinophilin regulates Ca2+ signalling by binding the N-terminal domain of RGS2 and the third intracellular loop of G-protein-coupled receptors
    Xinhua Wang
    Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390 9040, USA
    Nat Cell Biol 7:405-11. 2005
    ..These findings provide a general mechanism by which RGS proteins recognize GPCRs to confer signalling specificity...
  13. pmc WNK1 promotes PIP₂ synthesis to coordinate growth factor and GPCR-Gq signaling
    Sung Wan An
    Department of Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Curr Biol 21:1979-87. 2011
    ..WNK1 protein kinase is known to regulate ion homeostasis and cause hypertension when expression is increased by gene mutations. However, its signaling functions remain largely elusive...
  14. ncbi request reprint Quantitative assays for GTPase-activating proteins
    Elliott M Ross
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Methods Enzymol 344:601-17. 2002
  15. ncbi request reprint Modulation of the affinity and selectivity of RGS protein interaction with G alpha subunits by a conserved asparagine/serine residue
    B A Posner
    Department of Pharmacology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235 9041, USA
    Biochemistry 38:7773-9. 1999
    ..Detection of GAP activities of the mutants was enhanced in sensitivity up to 100-fold by assay at steady state in proteoliposomes that contain heterotrimeric G protein and receptor...
  16. ncbi request reprint Inhibition of brain Gz GAP and other RGS proteins by palmitoylation of G protein alpha subunits
    Y Tu
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235 9041, USA
    Science 278:1132-5. 1997
    ..Reversible palmitoylation is thus a major determinant of Gz deactivation after its stimulation by receptors, and may be a general mechanism for prolonging or potentiating G-protein signaling...
  17. ncbi request reprint Palmitoylation of a conserved cysteine in the regulator of G protein signaling (RGS) domain modulates the GTPase-activating activity of RGS4 and RGS10
    Y Tu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041, USA
    J Biol Chem 274:38260-7. 1999
    ..Dual palmitoylation of wild-type RGS4 remained inhibitory. RGS protein palmitoylation is thus multi-site, complex in its control, and either inhibitory or stimulatory depending on the RGS protein and its sites of palmitoylation...
  18. ncbi request reprint Phospholipase C-beta1 directly accelerates GTP hydrolysis by Galphaq and acceleration is inhibited by Gbeta gamma subunits
    P Chidiac
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75235 9041, USA
    J Biol Chem 274:19639-43. 1999
    ....
  19. pmc Use of a cAMP BRET sensor to characterize a novel regulation of cAMP by the sphingosine 1-phosphate/G13 pathway
    Lily I Jiang
    Department of Pharmacology, Alliance for Cellular Signaling, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9196, USA
    J Biol Chem 282:10576-84. 2007
    ..Thus in these macrophage cells, all four major classes of G proteins can regulate intracellular cAMP...
  20. doi request reprint Mammalian phospholipase C
    Ganesh Kadamur
    Department of Pharmacology, Molecular Biophysics Graduate Program and Green Center for Systems Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA
    Annu Rev Physiol 75:127-54. 2013
    ....
  21. ncbi request reprint Mutations in the carboxyl-terminal domain of phospholipase C-beta 1 delineate the dimer interface and a potential Galphaq interaction site
    Olga Ilkaeva
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9041
    J Biol Chem 277:4294-300. 2002
    ..Its location in the molecule suggests that moving the attachment point of the catalytic domain can disrupt its ability to be activated by Galpha(q)...
  22. ncbi request reprint RGSZ1 and Ret RGS: two of several splice variants from the gene RGS20
    S A Barker
    Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390 9041, USA
    Genomics 78:223-9. 2001
    ..At least two mRNAs that include the exon that encodes the N-terminal region unique to RGSZ1 were found in brain and a few other tissues, but not retina. RGS20 thus can account for multiple G(z)-selective GAPs in different tissues...
  23. ncbi request reprint Binding of regulator of G protein signaling (RGS) proteins to phospholipid bilayers. Contribution of location and/or orientation to Gtpase-activating protein activity
    Y Tu
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9041, USA
    J Biol Chem 276:20160-6. 2001
    ..The RGS4 N-terminal domain confers similar membrane binding behavior on the RGS domains of either RGS10 or RGSZ1...
  24. ncbi request reprint Phospholipase C-beta 1 is a GTPase-activating protein for Gq/11, its physiologic regulator
    G Berstein
    Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas 75235 9041
    Cell 70:411-8. 1992
    ..Such GAP activity by an effector coupled to a trimeric G protein can reconcile slow GTP hydrolysis by pure G proteins in vitro with fast physiologic deactivation of G protein-mediated signaling...
  25. ncbi request reprint Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells
    E M Parker
    Department of Pharmacology, Southwestern Graduate School of Biomedical Sciences, University of Texas Southwestern Medical Center, Dallas 75235 9041
    J Biol Chem 266:519-27. 1991
    ..The beta-AR and m2 MAChR were characteristically stimulated by reduction of disulfides. These results demonstrate the general utility of the baculovirus system for production of large quantities of native G protein-coupled receptors...