Steven D Rosen

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Measuring the activities of the Sulfs: two novel heparin/heparan sulfate endosulfatases
    Kenji Uchimura
    Department of Anatomy, Program in Immunology, UCSF, USA
    Methods Enzymol 416:243-53. 2006
  2. pmc Sulf-2: an extracellular modulator of cell signaling and a cancer target candidate
    Steven D Rosen
    University of California, Department of Anatomy and Comprehensive Cancer Center, San Francisco, 94143, USA
    Expert Opin Ther Targets 14:935-49. 2010
  3. ncbi request reprint Homing in on L-selectin
    Steven D Rosen
    Department of Anatomy and Program in Immunology, University of California San Francisco, San Francisco, CA 94143, USA
    J Immunol 177:3-4. 2006
  4. pmc Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue
    Michael L Patnode
    From the Department of Anatomy and Program in Biomedical Sciences, University of California, San Francisco, California 94143 0452
    J Biol Chem 288:26533-45. 2013
  5. ncbi request reprint A major class of L-selectin ligands is eliminated in mice deficient in two sulfotransferases expressed in high endothelial venules
    Kenji Uchimura
    Department of Anatomy, Program in Immunology, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    Nat Immunol 6:1105-13. 2005
  6. pmc Therapeutic targeting of endothelial ligands for L-selectin (PNAd) in a sheep model of asthma
    Steven D Rosen
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    Am J Pathol 166:935-44. 2005
  7. pmc HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1
    Kenji Uchimura
    Department of Anatomy, Comprehensive Cancer Center, University of California, San Francisco, CA 94143 0452, USA
    BMC Biochem 7:2. 2006
  8. pmc Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins
    Sheena C Kerr
    Department of Anatomy, University of California, San Francisco, CA 94143, USA
    J Immunol 181:1480-90. 2008
  9. ncbi request reprint Sulfated L-selectin ligands as a therapeutic target in chronic inflammation
    Kenji Uchimura
    Department of Anatomy and Program in Immunology, University of California, San Francisco, CA 94143 0452, USA
    Trends Immunol 27:559-65. 2006
  10. pmc Detection of a sulfotransferase (HEC-GlcNAc6ST) in high endothelial venules of lymph nodes and in high endothelial venule-like vessels within ectopic lymphoid aggregates: relationship to the MECA-79 epitope
    Annette Bistrup
    Department of Anatomy, University of California, San Francisco, California 94143 0452, USA
    Am J Pathol 164:1635-44. 2004

Research Grants

Collaborators

Detail Information

Publications37

  1. ncbi request reprint Measuring the activities of the Sulfs: two novel heparin/heparan sulfate endosulfatases
    Kenji Uchimura
    Department of Anatomy, Program in Immunology, UCSF, USA
    Methods Enzymol 416:243-53. 2006
    ..The example illustrated is vascular endothelial growth factor (VEGF). This assay is projected to be very useful in the investigation of the biological functions of the Sulfs...
  2. pmc Sulf-2: an extracellular modulator of cell signaling and a cancer target candidate
    Steven D Rosen
    University of California, Department of Anatomy and Comprehensive Cancer Center, San Francisco, 94143, USA
    Expert Opin Ther Targets 14:935-49. 2010
    ..The Sulfs are dysregulated in many cancers with Sulf-2 in particular implicated as a driver of carcinogenesis in NSCLC, pancreatic cancer and hepatocellular carcinoma...
  3. ncbi request reprint Homing in on L-selectin
    Steven D Rosen
    Department of Anatomy and Program in Immunology, University of California San Francisco, San Francisco, CA 94143, USA
    J Immunol 177:3-4. 2006
  4. pmc Galactose 6-O-sulfotransferases are not required for the generation of Siglec-F ligands in leukocytes or lung tissue
    Michael L Patnode
    From the Department of Anatomy and Program in Biomedical Sciences, University of California, San Francisco, California 94143 0452
    J Biol Chem 288:26533-45. 2013
    ....
  5. ncbi request reprint A major class of L-selectin ligands is eliminated in mice deficient in two sulfotransferases expressed in high endothelial venules
    Kenji Uchimura
    Department of Anatomy, Program in Immunology, Cardiovascular Research Institute, University of California, San Francisco, California 94143, USA
    Nat Immunol 6:1105-13. 2005
    ..Our results establish an essential function for the sulfotransferases in L-selectin ligand synthesis and may have relevance for therapy of inflammatory diseases...
  6. pmc Therapeutic targeting of endothelial ligands for L-selectin (PNAd) in a sheep model of asthma
    Steven D Rosen
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    Am J Pathol 166:935-44. 2005
    ..Furthermore, MECA-79 inhibited the accumulation of all classes of leukocytes in bronchoalveolar lavage fluid. These findings represent the first demonstration that targeting of PNAd has therapeutic efficacy in an inflammatory disease...
  7. pmc HSulf-2, an extracellular endoglucosamine-6-sulfatase, selectively mobilizes heparin-bound growth factors and chemokines: effects on VEGF, FGF-1, and SDF-1
    Kenji Uchimura
    Department of Anatomy, Comprehensive Cancer Center, University of California, San Francisco, CA 94143 0452, USA
    BMC Biochem 7:2. 2006
    ..To determine whether HSulf-2 modulates the interactions between heparin-binding factors and heparin, we developed an ELISA, in which soluble factors were allowed to bind to immobilized heparin...
  8. pmc Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins
    Sheena C Kerr
    Department of Anatomy, University of California, San Francisco, CA 94143, USA
    J Immunol 181:1480-90. 2008
    ..These in vitro findings indicate that EG can function as a ligand for the vascular selectins...
  9. ncbi request reprint Sulfated L-selectin ligands as a therapeutic target in chronic inflammation
    Kenji Uchimura
    Department of Anatomy and Program in Immunology, University of California, San Francisco, CA 94143 0452, USA
    Trends Immunol 27:559-65. 2006
    ..Experiments in a sheep model of asthma demonstrate that a chronic inflammatory disease can be ameliorated by targeting PNAd...
  10. pmc Detection of a sulfotransferase (HEC-GlcNAc6ST) in high endothelial venules of lymph nodes and in high endothelial venule-like vessels within ectopic lymphoid aggregates: relationship to the MECA-79 epitope
    Annette Bistrup
    Department of Anatomy, University of California, San Francisco, California 94143 0452, USA
    Am J Pathol 164:1635-44. 2004
    ..Analysis of the progeny of RIP-BLC by HEC-GlcNAc6ST-null mice establishes that the enzyme is responsible for the MECA-79 defined luminal ligands...
  11. pmc Functional contributions of N- and O-glycans to L-selectin ligands in murine and human lymphoid organs
    Hanayo Arata-Kawai
    Department of Anatomy, University of California, San Francisco, California 94143 0452, USA
    Am J Pathol 178:423-33. 2011
    ..These findings extend the evidence for the importance of N-glycans in lymphocyte homing in mouse and indicate that this dependency also applies to human lymphoid organs...
  12. pmc KSGal6ST generates galactose-6-O-sulfate in high endothelial venules but does not contribute to L-selectin-dependent lymphocyte homing
    Michael L Patnode
    Department of Anatomy and Program in Biomedical Sciences, University of California, San Francisco, CA 94143 0452, USA
    Glycobiology 23:381-94. 2013
    ..These results refine our understanding of the biological ligands for L-selectin and introduce a mouse model for investigating the functions of Gal6S in other contexts...
  13. pmc Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer
    Megumi Morimoto-Tomita
    Department of Anatomy and the UCSF Comprehensive Cancer Center, University of California, San Francisco, 94143 0452, USA
    Neoplasia 7:1001-10. 2005
    ..In support of the possible mechanistic relevance of Sulf-2 upregulation in tumors, purified recombinant Sulf-2 promoted angiogenesis in the chick chorioallantoic membrane assay...
  14. pmc Heparan sulfate sulfatase SULF2 regulates PDGFRα signaling and growth in human and mouse malignant glioma
    Joanna J Phillips
    Department of Neurological Surgery, UCSF, San Francisco, California 94158, USA
    J Clin Invest 122:911-22. 2012
    ..Our results suggest the bioavailability of growth factors from the microenvironment is a significant contributor to tumor growth in a major subset of human GBM...
  15. pmc Extracellular sulfatases, elements of the Wnt signaling pathway, positively regulate growth and tumorigenicity of human pancreatic cancer cells
    Roman Nawroth
    Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 2:e392. 2007
    ..Emerging evidence indicates the importance of reactivated Wnt signaling in a number of cancers, including pancreatic adenocarcinoma...
  16. pmc Functional consequences of the subdomain organization of the sulfs
    Renhong Tang
    Department of Anatomy, University of California, San Francisco, California 94143, USA
    J Biol Chem 284:21505-14. 2009
    ..The uncleavable Sulfs retained enzymatic activity. However, they were unable to potentiate Wnt signaling, which may be due to their defective localization into lipid rafts on the plasma membrane...
  17. pmc Gene trap disruption of the mouse heparan sulfate 6-O-endosulfatase gene, Sulf2
    David H Lum
    Department of Anatomy, HSW 1323, University of California San Francisco, CA 94143 0452, USA
    Mol Cell Biol 27:678-88. 2007
    ..These data suggest that Sulf2 may have roles in several tissues but that there is compensation by and/or redundancy with Sulf1...
  18. ncbi request reprint Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x
    Claudia B Fieger
    Department of Anatomy, Program in Immunology, University of California, San Francisco, California 94143 0452, USA
    J Biol Chem 278:27390-8. 2003
    ..Since the distribution of endoglycan includes hematopoietic precursors and leukocyte subpopulations, in addition to endothelial cells, our findings suggest several potential settings for endoglycan-mediated adhesion events...
  19. ncbi request reprint Ligands for L-selectin: homing, inflammation, and beyond
    Steven D Rosen
    Department of Anatomy and Program in Immunology, University of California, San Francisco, San Francisco, California 94143 0452, USA
    Annu Rev Immunol 22:129-56. 2004
    ..The rapid "homing" of labeled cells into the lymph nodes presumably had its basis in the special affinity of small lymphocytes for the endothelium of the postcapillary venules...
  20. pmc Lymphocyte-HEV interactions in lymph nodes of a sulfotransferase-deficient mouse
    Annemieke van Zante
    Department of Anatomy, Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA
    J Exp Med 198:1289-300. 2003
    ..Our results demonstrate that HEC-GlcNAc6ST contributes predominantly, but not exclusively, to the sulfation of HEV ligands for L-selectin and that alternative, non-MECA79-reactive ligands are present in the absence of HEC-GlcNAc6ST...
  21. pmc The SULFs, extracellular sulfatases for heparan sulfate, promote the migration of corneal epithelial cells during wound repair
    Inna Maltseva
    Department of Anatomy, University of California San Francisco, San Francisco, California, United States of America
    PLoS ONE 8:e69642. 2013
    ..Further understanding of the mechanistic action of the SULFs in promoting corneal repair may lead to new therapeutic approaches for the treatment of corneal injuries...
  22. pmc Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells
    Yafeng Zhang
    Department of Anatomy, University of California San Francisco, San Francisco, CA 94143, USA
    J Immunol 189:3914-24. 2012
    ..This entry role for LPA complements the efflux function of sphingosine 1-phosphate...
  23. pmc Cloning and characterization of two extracellular heparin-degrading endosulfatases in mice and humans
    Megumi Morimoto-Tomita
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    J Biol Chem 277:49175-85. 2002
    ..Taken together, our results establish that the mammalian Sulfs are extracellular endosulfatases with strong potential for modulating the interactions of heparan sulfate proteoglycans in the extracellular microenvironment...
  24. pmc Leukotriene B4 amplifies eosinophil accumulation in response to nematodes
    Michael L Patnode
    Department of Anatomy, Program in Biomedical Sciences, Howard Hughes Medical Institute, Department of Medicine, and Department of Pathology, University of California, San Francisco, San Francisco, CA 94143Department of Anatomy, Program in Biomedical Sciences, Howard Hughes Medical Institute, Department of Medicine, and Department of Pathology, University of California, San Francisco, San Francisco, CA 94143
    J Exp Med 211:1281-8. 2014
    ..The similarity of the eosinophil responses to diverse nematode species suggests that conserved features of nematodes are recognized during parasite infection...
  25. pmc SULF2 expression by immunohistochemistry and overall survival in oesophageal cancer: a cohort study
    Natalie Shaubie Lui
    Thoracic Oncology Laboratory, Department of Surgery, University of California San Francisco, San Francisco, California, USA
    BMJ Open 2:. 2012
    ..This study evaluates the relationship between SULF2 expression by immunohistochemistry and overall survival in patients with oesophageal cancer...
  26. pmc Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs
    Hidenobu Kanda
    Department of Anatomy, Program in Immunology, Cardiovascular Research Institute, University of California San Francisco, San Francisco, California 94143, USA
    Nat Immunol 9:415-23. 2008
    ..Our results support the idea of a new and general step in the homing cascade in which the ectoenzyme ATX facilitates the entry of lymphocytes into lymphoid organs...
  27. ncbi request reprint Trophoblast L-selectin-mediated adhesion at the maternal-fetal interface
    Olga D Genbacev
    Departments of Stomatology, Anatomy, and Pharmaceutical Chemistry, University of California, San Francisco, CA 94143, USA
    Science 299:405-8. 2003
    ..These results suggest that trophoblast L-selectin mediates interactions with the uterus and that this adhesion mechanism may be critical to establishing human pregnancy...
  28. ncbi request reprint Differential gene expression profile of human tonsil high endothelial cells: implications for lymphocyte trafficking
    Diana Palmeri
    Department of Anatomy and Program of Immunology, University of California, San Francisco, USA
    J Leukoc Biol 75:910-27. 2004
    ..The library contains a great diversity of transcripts, and studies of the encoded proteins will provide further insight into the complex biology of these specialized endothelial cells...
  29. ncbi request reprint Compositional profiling of heparin/heparan sulfate using mass spectrometry: assay for specificity of a novel extracellular human endosulfatase
    Ola M Saad
    Department of Chemistry, University of California, Berkeley, CA 94720, USA
    Glycobiology 15:818-26. 2005
    ..Proof of principle was performed by using various heparin/HS samples isolated from bovine and porcine tissues...
  30. ncbi request reprint Activities and expression pattern of the carbohydrate sulfotransferase GlcNAc6ST-3 (I-GlcNAc6ST): functional implications
    Jin Kyu Lee
    Department of Anatomy, University of California, San Francisco, CA 94143 0452, USA
    Glycobiology 13:245-54. 2003
    ..These latter findings argue that GlcNAc6ST-3 is not involved in generating HEV-expressed ligands for L-selectin...
  31. ncbi request reprint The use of flow cytometry to assess neutrophil infiltration in the injured murine spinal cord
    Tjoson Tjoa
    Department of Neurological Surgery, University of California, San Francisco, CA 94143, USA
    J Neurosci Methods 129:49-59. 2003
    ..Together, these findings demonstrate an effective method for the detection and quantification of viable neutrophils in the injured murine spinal cord...
  32. pmc Induction of PNAd and N-acetylglucosamine 6-O-sulfotransferases 1 and 2 in mouse collagen-induced arthritis
    Jiwei Yang
    Thios Pharmaceuticals Inc, P, O, Box 20010, Oakland, CA 94620, USA
    BMC Immunol 7:12. 2006
    ..Induction of PNAd has also been shown to occur in a number of human inflammatory diseases including rheumatoid arthritis (RA)...
  33. ncbi request reprint N-acetylglucosamine 6-O-sulfotransferase-1 regulates expression of L-selectin ligands and lymphocyte homing
    Kenji Uchimura
    Department of Biochemistry, Nagoya University Graduate School of Medicine, 65 Tsurumai Cho, Showa Ku, Nagoya 466 8550, Japan
    J Biol Chem 279:35001-8. 2004
    ..The importance of GlcNAc6ST-1 is particularly high-lighted by its involvement in lymphocyte homing to Peyer's patches where GlcNAc6ST-2 expression is undetectable...
  34. ncbi request reprint Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes
    Jean Marc Gauguet
    CBR Institute for Biomedical Research, Department of Pathology, Harvard Medical School, Boston, MA, USA
    Blood 104:4104-12. 2004
    ..These results demonstrate distinct functions for C2GlcNAcT-I and HEC-GlcNAc6ST in the differential elaboration of HEV glycoproteins that set a threshold for the amount of L-selectin needed for lymphocyte homing...
  35. pmc A HEV-restricted sulfotransferase is expressed in rheumatoid arthritis synovium and is induced by lymphotoxin-alpha/beta and TNF-alpha in cultured endothelial cells
    Jose L Pablos
    Servicio de Reumatología y Unidad de Investigación, Hospital 12 de Octubre, 28041 Madrid, Spain
    BMC Immunol 6:6. 2005
    ....
  36. ncbi request reprint Low force decelerates L-selectin dissociation from P-selectin glycoprotein ligand-1 and endoglycan
    Krishna K Sarangapani
    Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332, USA
    J Biol Chem 279:2291-8. 2004
    ..By contrast, only slip bonds were observed for L-selectin-antibody interactions. These findings suggest that catch bonds contribute to the shear threshold for rolling and are a common characteristic of selectin-ligand interactions...
  37. ncbi request reprint Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs
    Krystle M Veerman
    The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    Nat Immunol 8:532-9. 2007
    ..Our data show involvement of PSGL-1 in facilitating the entry of T cells into secondary lymphoid organs, thereby demonstrating the bifunctional nature of this molecule...

Research Grants1

  1. Sulfotransferases in the Synthesis of L-Selectin Ligands
    Steven D Rosen; Fiscal Year: 2010
    ..Understanding this mechanism may lead to new pharmacologic approaches for dampening eosinophil responses. ..