Ellen A Robey

Summary

Affiliation: University of California
Country: USA

Publications

  1. ncbi request reprint Visualizing thymocyte motility using 2-photon microscopy
    Ellen A Robey
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Immunol Rev 195:51-7. 2003
  2. pmc Dynamics of T cell, antigen-presenting cell, and pathogen interactions during recall responses in the lymph node
    Tatyana Chtanova
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Immunity 31:342-55. 2009
  3. ncbi request reprint Thymocyte-dendritic cell interactions near sources of CCR7 ligands in the thymic cortex
    Ena Ladi
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Immunol 181:7014-23. 2008
  4. ncbi request reprint CCR7 expression in developing thymocytes is linked to the CD4 versus CD8 lineage decision
    Xinye Yin
    Department of Molecular and Cell Biology, Division of Immunology, University of California, Berkeley, CA 94720, USA
    J Immunol 179:7358-64. 2007
  5. doi request reprint Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii
    Marie Schaeffer
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Immunol 182:6379-93. 2009
  6. pmc Distinct temporal patterns of T cell receptor signaling during positive versus negative selection in situ
    Heather J Melichar
    1Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Sci Signal 6:ra92. 2013
  7. pmc The impact of negative selection on thymocyte migration in the medulla
    Marie Le Borgne
    Department of Molecular and Cell Biology, University of California, Berkeley, USA
    Nat Immunol 10:823-30. 2009
  8. ncbi request reprint Impairment of thymocyte development by dominant-negative Kuzbanian (ADAM-10) is rescued by the Notch ligand, delta-1
    Jennifer O Manilay
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    J Immunol 174:6732-41. 2005
  9. pmc Opposing chemokine gradients control human thymocyte migration in situ
    Joanna Halkias
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, UC Berkeley, Berkeley, California 94720 3200, USA
    J Clin Invest 123:2131-42. 2013
  10. pmc Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread
    Janine L Coombes
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 110:E1913-22. 2013

Collaborators

Detail Information

Publications38

  1. ncbi request reprint Visualizing thymocyte motility using 2-photon microscopy
    Ellen A Robey
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Immunol Rev 195:51-7. 2003
    ..In this review, we discuss the implications of thymocyte motility for T-cell repertoire selection and for the mechanisms that determine the spatial organization of thymocyte subsets within the thymus...
  2. pmc Dynamics of T cell, antigen-presenting cell, and pathogen interactions during recall responses in the lymph node
    Tatyana Chtanova
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Immunity 31:342-55. 2009
    ..Our results provide insight into cellular interactions during recall responses and suggest a mechanism of pathogen subversion of the immune response...
  3. ncbi request reprint Thymocyte-dendritic cell interactions near sources of CCR7 ligands in the thymic cortex
    Ena Ladi
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Immunol 181:7014-23. 2008
    ..These observations identify a vasculature-associated microenvironment within the thymic cortex that promotes interactions between DCs and thymocytes that are receiving positive selection signals...
  4. ncbi request reprint CCR7 expression in developing thymocytes is linked to the CD4 versus CD8 lineage decision
    Xinye Yin
    Department of Molecular and Cell Biology, Division of Immunology, University of California, Berkeley, CA 94720, USA
    J Immunol 179:7358-64. 2007
    ..These findings suggest that the timing of CCR7 expression relative to coreceptor down-regulation is regulated by lineage commitment signals...
  5. doi request reprint Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii
    Marie Schaeffer
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Immunol 182:6379-93. 2009
    ..Our data represent the first direct observation of the dynamics of T cell-parasite interactions within living tissue and provide a new perspective for understanding immune responses to persistent pathogens in the brain...
  6. pmc Distinct temporal patterns of T cell receptor signaling during positive versus negative selection in situ
    Heather J Melichar
    1Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA
    Sci Signal 6:ra92. 2013
    ..Thus, we provide a comparison of positive and negative selection signals in situ and suggest that the absence of strong stop signals distinguishes between positive and negative selection. ..
  7. pmc The impact of negative selection on thymocyte migration in the medulla
    Marie Le Borgne
    Department of Molecular and Cell Biology, University of California, Berkeley, USA
    Nat Immunol 10:823-30. 2009
    ..Our data suggest that many autoreactive thymocytes do not undergo immediate arrest and death after encountering a negative selecting ligand but instead adopt an altered migration program while remaining in the medullary microenvironment...
  8. ncbi request reprint Impairment of thymocyte development by dominant-negative Kuzbanian (ADAM-10) is rescued by the Notch ligand, delta-1
    Jennifer O Manilay
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    J Immunol 174:6732-41. 2005
    ..Our data suggest that multiple Notch-dependent steps in early thymocyte development require Kuzbanian, but differ in the involvement of other Notch signaling components...
  9. pmc Opposing chemokine gradients control human thymocyte migration in situ
    Joanna Halkias
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, UC Berkeley, Berkeley, California 94720 3200, USA
    J Clin Invest 123:2131-42. 2013
    ....
  10. pmc Motile invaded neutrophils in the small intestine of Toxoplasma gondii-infected mice reveal a potential mechanism for parasite spread
    Janine L Coombes
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 110:E1913-22. 2013
    ..gondii infection through the lumen of the intestine. Our data identify neutrophils as motile reservoirs of T. gondii infection and suggest a surprising retrograde pathway for parasite spread in the intestine...
  11. pmc Distinct phases in the positive selection of CD8+ T cells distinguished by intrathymic migration and T-cell receptor signaling patterns
    Jenny O Ross
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3200 and
    Proc Natl Acad Sci U S A 111:E2550-8. 2014
    ....
  12. pmc Infection-induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus
    Janine L Coombes
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USA
    Cell Rep 2:124-35. 2012
    ..Our data reveal pathways through which NK cell migration and function are regulated after infection and identify an important accessory cell population for activation of NK cell responses in lymph nodes...
  13. doi request reprint Quantifying subcellular distribution of fluorescent fusion proteins in cells migrating within tissues
    Heather J Melichar
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
    Immunol Cell Biol 89:549-57. 2011
    ..These approaches could be readily adapted to other fluorescent fusion proteins, tissues and biological questions...
  14. ncbi request reprint Immunodominant, protective response to the parasite Toxoplasma gondii requires antigen processing in the endoplasmic reticulum
    Nicolas Blanchard
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Immunol 9:937-44. 2008
    ..gondii-infected ERAAP-deficient mice, which were more susceptible to toxoplasmosis. Thus, endoplasmic reticulum proteolysis is critical for eliciting protective immunity to a vacuolar parasite...
  15. pmc The Toxoplasma gondii peptide AS15 elicits CD4 T cells that can control parasite burden
    Harshita Satija Grover
    Division of Immunology and Pathogenesis, Department of Molecular and Cellular Biology, University of California, Berkeley, California, USA
    Infect Immun 80:3279-88. 2012
    ....
  16. pmc Directed migration of positively selected thymocytes visualized in real time
    Colleen M Witt
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    PLoS Biol 3:e160. 2005
    ..These studies provide our first glimpse into the dynamics of developmentally programmed, long-range cell migration in the mammalian thymus...
  17. pmc Fas-associated death domain (FADD) is a negative regulator of T-cell receptor-mediated necroptosis
    Stephanie L Osborn
    Cancer Research Laboratory and Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 107:13034-9. 2010
    ..We conclude that FADD constitutes a mechanism to keep TCR-induced programmed necrotic signaling in check during early phases of T-cell clonal expansion...
  18. ncbi request reprint Thymic microenvironments for T cell differentiation and selection
    Ena Ladi
    Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Nat Immunol 7:338-43. 2006
    ..In addition, we discuss the diverse developmental signals provided by these microenvironments that contribute to the generation of divergent T cell lineages...
  19. pmc Quantitative and temporal requirements revealed for Zap70 catalytic activity during T cell development
    Byron B Au-Yeung
    1 Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, California, USA 2 Rosalind Russell Ephraim P Engleman Rheumatology Research Center, University of California, San Francisco, San Francisco, California, USA 3 Department of Medicine, University of California, San Francisco, San Francisco, California, USA 4 Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California, USA 5
    Nat Immunol 15:687-94. 2014
    ....
  20. ncbi request reprint The Notch regulator Numb links the Notch and TCR signaling pathways
    Ana C Anderson
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA
    J Immunol 174:890-7. 2005
    ..Together our data suggest that Notch and TCR signals may be integrated at the cell membrane, and that Numb may be an important adapter in this process...
  21. ncbi request reprint Thymopoiesis in 4 dimensions
    Colleen M Witt
    Division of Immunology, Department of Molecular and Cell Biology, 479 Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Semin Immunol 17:95-102. 2005
    ..We also discuss new imaging technologies and interdisciplinary approaches and discuss how the promise they offer to addressing these questions...
  22. pmc Impact of regulated secretion on antiparasitic CD8 T cell responses
    Harshita Satija Grover
    Division of Immunology and Pathogenesis, Department of Molecular and Cellular Biology, University of California, Berkeley, Berkeley, CA 94720 3200, USA
    Cell Rep 7:1716-28. 2014
    ..CD8 T cell responses to the dense-granule-targeted ROP5 epitope resulted in reduced parasite load in the brain. These data suggest that the mode of secretion affects the efficacy of parasite-specific CD8 T cell responses. ..
  23. pmc Two-photon imaging of the immune system
    Ivan L Dzhagalov
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, USA
    Curr Protoc Cytom . 2012
    ..In this unit, we provide several protocols for preparation of various organs for imaging by two-photon microscopy that are intended to introduce the new user to some basic aspects of this method...
  24. ncbi request reprint Thymocyte motility: mutants, movies and migration patterns
    Xinye Yin
    Division of Immunology, Department of Molecular and Cell Biology, 471 Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Curr Opin Immunol 18:191-7. 2006
    ....
  25. pmc Dynamics of neutrophil migration in lymph nodes during infection
    Tatyana Chtanova
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA
    Immunity 29:487-96. 2008
    ..Our data provide insights into the cellular mechanisms underlying neutrophil swarming and suggest new roles for neutrophils in shaping immune responses...
  26. pmc Comparative study of hematopoietic differentiation between human embryonic stem cell lines
    Heather Melichar
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America
    PLoS ONE 6:e19854. 2011
    ..These data underscore the difficulties in the current strategy of hESC forward differentiation and highlight distinct differences between CD34(+) hematopoietic precursors generated in vitro versus in vivo...
  27. doi request reprint Dynamic imaging of host-pathogen interactions in vivo
    Janine L Coombes
    Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, California 94720, USA
    Nat Rev Immunol 10:353-64. 2010
    ..Here we discuss how dynamic imaging of in vivo infection has revealed fascinating and unexpected details of host-pathogen interactions at a new level of spatial and temporal resolution...
  28. pmc Dynamic repositioning of CD4 and CD8 genes during T cell development
    Stephanie Delaire
    Department of Molecular and Cell Biology, 475 Life Science Additions, University of California, Berkeley, Berkeley, CA 94720, USA
    J Exp Med 200:1427-35. 2004
    ..We discuss our results in terms of our current knowledge of CD4 and CD8 gene regulation and CD4 versus CD8 lineage commitment...
  29. ncbi request reprint Tracking migration during human T cell development
    Joanna Halkias
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, 142 Life Sciences Addition, 3200, Berkeley, CA, 94720 3200, USA
    Cell Mol Life Sci 71:3101-17. 2014
    ..Here we discuss the dynamic changes in behavior that occur throughout thymocyte development, and provide an overview of the cell-intrinsic and extrinsic mechanisms that regulate human thymocyte migration. ..
  30. pmc Elimination of self-reactive T cells in the thymus: a timeline for negative selection
    Ivan Lilyanov Dzhagalov
    Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, University of California, Berkeley, California, United States of America
    PLoS Biol 11:e1001566. 2013
    ..Our data provide a timeline for negative selection and reveal close coupling between cell death and clearance in the thymus...
  31. pmc Class I major histocompatibility complex presentation of antigens that escape from the parasitophorous vacuole of Toxoplasma gondii
    Marc Jan Gubbels
    Department of Molecular and Cell Biology, 471 Life Sciences Addition, University of California Berkeley, Berkeley, CA 94720, USA
    Infect Immun 73:703-11. 2005
    ....
  32. pmc The ins and outs of CCR7 in the thymus
    Colleen M Witt
    Department of Molecular and Cell Biology, 471 Life Sciences Addition, University of California, Berkeley 94720, USA
    J Exp Med 200:405-9. 2004
    ..Together these papers provide a fascinating picture of the complex role of CCR7 in orchestrating thymocyte migration...
  33. ncbi request reprint Notch signaling in lymphocyte development and function
    Ellen A Robey
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA
    Curr Opin Immunol 16:360-6. 2004
    ..Finally, recent studies have provided our first glimpse into the complex and paradoxical roles of Notch signaling in the activation and differentiation of mature T cells...
  34. pmc Distinct transcriptional programs in thymocytes responding to T cell receptor, Notch, and positive selection signals
    Yina H Huang
    Department of Molecular and Cell Biology, Division of Immunology and Cancer Research Laboratory, 475 LSA, University of California, Berkeley, CA 94720, USA
    Proc Natl Acad Sci U S A 101:4936-41. 2004
    ..These results help to identify TCR- and positive-selection-specific transcriptional events and help to clarify the relationship between positive selection and Notch...
  35. pmc 3D quantitative imaging of unprocessed live tissue reveals epithelial defense against bacterial adhesion and subsequent traversal requires MyD88
    Connie Tam
    School of Optometry, University of California, Berkeley, California, United States of America
    PLoS ONE 6:e24008. 2011
    ....
  36. ncbi request reprint Immunology: guide for a cell-fate decision
    Ellen A Robey
    Nature 433:813-4. 2005
  37. ncbi request reprint Dynamic behavior of T cells and thymocytes in lymphoid organs as revealed by two-photon microscopy
    Philippe Bousso
    Unité U277, Departement d Immunologie, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France
    Immunity 21:349-55. 2004
    ..In this review we summarize what we have learned so far and point out areas for future investigation using this approach...
  38. ncbi request reprint A reassessment of the effect of activated Notch1 on CD4 and CD8 T cell development
    B J Fowlkes
    Laboratory of Cellular and Molecular Immunology, National Institutes of Health, Bethesda, MD 20892, USA
    J Immunol 169:1817-21. 2002
    ..We suggest that the discrepancies in previous reports of Notch1IC transgenic mice are due to differences in the propensity of the two different transgenic lines to develop tumors...